ContentsNo 1 JANUARY 1988 Page

Gerard lvi:Slavin:rnl f liicEditor,y 97J98ou........al...of....Cl.......cal....Pa....hology.......1978.....1988......1Topographical and histological presentation of mammographic pathology in breast cancer NM GIBBS.......................... 3Use ofimmunohistochemical staining panel forcharacterisation ofovarian neoplasms P ASHORN, MHELLE, HHELIN, RASHORN, KKROHN 12Ultrastructural and immunocytochemical study of a case of invasive cribriform breast carcinoma CA WELLS, DiP FERGUSON........ 17Retrospective study of prognostic importance of DNA flow cytometry of urinary bladder carcinoma CEM BLOMJOUS, NW SCHIPPER,JPA BAAK, EM VAN GALEN, Hi DE VOOGT, CJLM MEYER................................................................. 21Histopathology of benign non-palpable breast lesions identified by mammography NICOLA J BARNARD, BD GEORGE, AK TUCKER,OJJA M R ......... G.............................LM ORE..........................................26............ 2Nucleolar organiser regions in fibrous proliferation's of childhood and infantile fibrosarcoma MI EGAN, F RAAFAT, I CROCKER, K SMITH 31Brain damage in fatal non-missile head injury without high intracranial pressure DI GRAHAM, AUDREY E LAWRENCE, J HUME ADAMS,D O L , R M LLDOYLE,............................DR............M........LE..................AN............34...... 3Application of radioimmunoassay to monitor treatment of human cerebral gliomas with bleomycin entrapped within liposomesGB FIRTH, MARIA FIRTH, RO MCKERAN, J REES, P WALTER, D UTTLEY, V MARKS ............................................... 38Serum C reactive protein in infective endocarditiS A CHRISTINE MCCARTNEY, GILLIAN V ORANGE, SD PRINGLE, G WILLS, U REECE ....... 44Characterisation ofconnective tissuecellscontaining factorXlll subunit a R6ZA ADANY, MARINA AGLUKHOVA, AY KABAKOV, LMUSZBEK 49Comparison of immunoperoxidase staining with indirect immunofluorescence, ELISA, and Western blotting assays for detectinganti-HTLV-1 antibodies in systemic lupus erythematosus K YAMAGUCHI, ESTELA MATUTES, T KIYOKAWA, YOKO NISHIMURA, T ISHII,K A A SU I C T VKAKAT.................UK...................D........CAT..............V.......KY...........57..... 5Fifty cases ofhuman immunodeficiency virus (HIV) infection: immunoultrastructural study ofcirculating lymphocytes wWWFEREMANS,K HUYGEN, R MENU, CM FARBER, JP DE CALUWE, JP VAN VOOREN, L MARCELIS, L ANDRE, H BRASSEUR, H BONDUEF, B LEBON, N CLUMECK 62What is colitis? Statistical approach to distinguishing clinically important inflammatory change in rectal biopsy specimenS D JENKINS,A O DA LGE ,BBS OTODALL,...................K.......DREW,.................BB.........SCOTT.................72....7Haemorrhagic colitis: detection of verotoxin producing Escherichia coli 0157 in a clinical microbiology laboratory CW WALKER,REBECCA N,R AR E ..................UPS.............N............RE..........WARREN......................80..... 8Campylobacter associated gastritis in patients with non-ulcer dyspepsia RJLF LOFFELD, HVPJ POr-ERS, JW ARENDS, E ST-OBBERINGH,JAF EN RG,J AFLENDRIG,..........................JP........VAN...........SPRE..............UWEL.............85....8Comparison between cell culture and serology for detecting Chlamydia trachomatis in women seeking abortion PA CSANG6, B SAROV,H C I T , A O .....CH...................T.....................I..............AR..............V..........89....... 8Tuberculous lymphadenitis associated with human immunodeficiency virus (HIV) in Uganda A NAMBUYA, N SEWANKAMBO,MUGERWA, G O GA E SL C S .............R.......GO........DGAME,...................S......LUCAS................93....9

Antimicrobial susceptibility testing of Neisseria gonorrhoeae: a trial organised as part of the United Kingdom national external qualityassessment mefo mcrbiloysJSheme DJ ROfor.....microbiology................1.....SNELL,........DF......BROWN.......97 9Technical methodsImmunoblot fingerprinting of coagulase negative staphylococci JP BURNIE, W LEE, RC MATTHEWS, R BAYSTON .................... 103fi-glucuronidase (PGUA) and fi-xylosidase (ONPX) disc tests for rapid identification of urinary coliformS NADIA AL-DERZI,RG MITC ...........................................................LL....................108................. 0LetrLethtetorsto........................................e....................t........................ll...... IInsCtuuct a t o s .....................................s.............................................116............ 1Boorvesk ..................................e...................ews.........................118............... 1Noteices ........................................................................1...................0....... 2

No 2 FEBRUARY 1988

Antisera against epitopes resistant to denaturation on T3 (CD3) antigen can detect reactive and neoplastic T cells in paraffin embeddedtissue biopsy specimenS DY MASON, GW KRISSANSEN, FR DAVEY, MI CRUMPTON, KC GATTER ................................... 121Immunoglobulin gene rearrangement and antigenic profile confirm B cell origin of primary cerebral lymphoma and indicate a maturephenotype WI SMIlTH, JA GARSON, SP BOURNE, JT KEMSHEAD, HB COAKHAM ................................................ 128Gonadal function in patients with fi thalassaemia major V DE SANCTIS, C VULLO, H KATZ, B WONKE, R TANAS, B BAGNI.............. 133Association between necropsy evidence of disseminated intravascular coagulation and coagulation variables before death in patients inintensiver uit JTWcareKMROEunitsGEAESJTPRSWNILDE......KM...ROBERTS,........H...GREAVES.........FE...PRESTON....... 13813Clinical application of new technique that measures C4d for assessment of activation of classical complement pathway ET DAVIES,BA NASARUDDIN, A ALHAQ, G SENALDI, D VERGANI.................................................................... 143Histological features of skin and rectal biopsy specimens after autologous and allogeneic bone marrow transplantationL SVILAND, ADJ PEARSON, EJ EASTHAM, PJ HAMILTON, Si PROCTOR, AJ MALCOLM AND THE NEWCASTLE UPON TYNE BONE MARROW TRANSPLANTGROUP.148.................................................................................................. 4An unusual interpodocyte cell junction and its appearance in a transplant graft kidney DJ HARRISON, D JENKINS, J DICK ............ 155Osteoarticular amyloidosis associated with haemodialysis: an immunoultrastructural study H DEPIERREUX, H GOLDMAN, ISABELLE FAYT,C ICARICHARD, , DAEE,JL ANHEWEHUINT........N...M...DHAENE............J-L......VAN......HERWEGHEM............. 158.5Routine and rapid enzyme linked immunosorbent assays for circulating anti-glomerular basement membrane antibodiesJANICE WHEELER, JUDY SIMPSON, AR MORLEY.163..................................................................... 6Breast lumps: rare presentation of oat cell carcinoma of lung C KELLY, 0 HENDERSON, P CORRIS............................... 171Morphometric analysis of breast carcinoma association with survival DM PARHAM, Al ROBERtTSON, RA BROWN.................... 173

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ClontentsWidespread expression of intestinal markers in gastric carcinoma: a light and electron microscopic study using BD-5 monoclonalantibody R FIOCCA, LAURA VILLANI, PATRIZIA TENTI, M CORNAGGIA, GIOVANNA FTNZI, C CAPELLA, MARIA PRAT, G BUSSOLATI, E SOLCIA.. 178Autosomal recessive distal myopathy H ISAACS, MARGARET E BADENHORST, T WHISTLER ..................................... 188Vitamin D deficiency and low osteocalcin concentrations in anorexia nervosa VA FONSECA, V D'SOUZA, SHARON HOULDER, M THOMAS,A WAKELING, P DANDONA...................195Reduced concentrations of selenium in mild Crohn's disease LESLEY J MINKS, KAREN D INWARDS, BARBARA LLOYD, BARBARA CLAYTON 198Quantitative method for determining serum alkaline phosphatase isoenzyme activity: estimation of intestinal component MJ PEAKE,M EA OVC HPEJAKOV.............................C............GH............WH...........TE...........202........ 0Prevention of nitroimidazole resistance in Campylobacterpylori by coadministration of colloidal bismuth subcitrate: clinical and in vitrostudies CS GOODWIN, BJ MARSHALL, ED BLINCOW, DH WILSON, S BLACKBOURN, M PHILLIPS .................................... 207Incidence of vaginal Weeksella virosa (formerly group Ilf) C MARDY, B HOLMES ........................................... 211Quality assessment in cervical cytology: a pilot study GDH THOMAS, C HEAD, J THOROGOOD .................................. 215Use of microcomputer for histopathology: system using IBM PC and dBase III WM SUEN, KW CHICK.......................... 220Comparison of hospital staff performance when using desk top analysers for "near patient" testing AA NANJI, R POON, I HINBERG 223Technical methodsSimplified screening procedure for detecting lupus inhibitor J GIBSN, ELIZABETH STARLING, LEONIE DATE, KA RICKARD, H KRONENBERG. 226Immunoturbidimetric assay for rheumatoid factor using an enzyme immunoassay microplate reader DJ DAMSN, NG CARTER........ 228Simple latex agglutination method for typing pneumococci AC LAFONG, E CROTH-ERS....................................... 230Letters h E io ........................to............the...............Editor..........................232........ 3Book v e s ...........................review s................................................236............... 3Notices.24................................................................................................. 04

No 3 MARCH 1988 Page

Polymorphonuclear leucocytes in Crohn's disease and ulcerative proctocolitis: association between enhanced adherence to nylon fibreand disease variables J CASON, CC AINLEY, RA WOLSTENCROFT, RPH THOMPSON ............................................ 241Clinical and biochemical assessments of damage due to perinatal asphyxia: a double blind trial of a quantitative method I LAING,JK B O N,R AR N S .BROWN,.........................RA................ARK..............ESS...............247........ 4Expression of major histocompatibility class II antigens by Langerhans' cells in cervical intraepithelial neoplasia RG HUGHES,H N V L,S M H WTORVAL,.....................................EM............HOW................E.........253......... 5Expression ofCEA, CAl125, CA19-9 and human milk fat globule membrane antigen in ovarian tumours FIONA MACDONALD, ROSALINDBIRD, HAZEL STOKES, BARBARA RUSSELL, J CROCKER .................................................................. 260Comparison of immunological detection of 5-hydroxytryptamine by monoclonal antibodies with standard silver stains as an aid todiagnosing odcarcinoid ......................................tumours...................AV............AW...... 265Detection of small cell lung cancer metastases in bone marrow aspirates using monoclonal antibody directed against neuroendocrinedifferentiation antigen HtH BERENDSEN, L DE LEIJ, PE POSTMUS, JG TER HAAR, S POPPEMA, TH THE................................ 273Nature of mononuclear cells positive for acid phosphatase activity in bone marrow of patients with renal osteodystrophy H KAYE,JANET DE S N ..........................HENDERSON.................................................277............ 7Malignant progression of laryngeal papilloma associated with human papilloma virus type 6 (HPV-6) DNA AP ZAROD,JD R T ER O D GCRRUTHERFORD,.....................................G.......C.......RB...................280.......... 8Human papillomavirus (HPV) genotypes associated with laryngeal papilloma G CORBIr, AP ZAROD, JR ARRAND, H LONGSON,WT F RGFARR .................................................GT..............................284.............. 8Detection of human papillomavirus DNA sequences by in situ DNA-DNA hybridisation in cervical intraepithelial neoplasia andinvasive carcinoma: a retrospective study JE COLLINS, D JENKINS, DJ MCCANCE ............................................ 289DNA hybridisation of cervical scrapes: comparison with cytological findings in Papanicolaou smears ANNE R MORSE, C wiCKENDEN,MICHELINE BYRNE, D TAYLOR-ROBINSON, JENNEFER SMITH, MC ANDERSON, CONAMORE SMITH, ADB MALCOLM, DULCIE V COLEMAN.......... 296Pure and mixed mucinous breast carcinomas: DNA stemline and prognosis S TOIKKANEN, E EEROLA, TO EKFORS .................. 300Immunohistological determination of proliferative activity in seminomas W DOJE, K-P DEECKMANN, V LOY....................... 304LectinndngtosebindingntuouS OtoRSROserous........ovarian.........tumour..........K.........DER.....TR....M... 308.30Immunocytochemical study of the cellular immune response in meningiomas ML ROSSI, F CRUZ SANCHEZ, rr HUGHES, MM ESIRI,MB C A H M..................COAK...........................................M..............314.................. 1Distribution of vitamin B12 R-binder in carcinomas of the digestive tract H KUTDO, G OHSHIO, K OGAWA, YC KIM, Y WAKATSUKI,Y A ASIANAKAAESHIADIMA......................H......YAMABE....................H......INADA...............320....2Use of lectin histochemistry in pancreatic cancer CK CHING, R BLACK, T HELLIWELL, A SAVAGE, H BARR, JM RHODES ................ 324Immunoglobulin G (IgG) serological response to Branhamella calarrhalis in patients with acute bronchopulmonary infectionsAJB A K,T I SO ...BLACK,............................T..............WILSON............................329......... 2Risk of hepatitis B infection among medical and paramedical workers in a general hospital in Zimbabwe JC EMMANUEL, MT BASSETT,HMSI H .........................M...................T.............................334........................ 3Technical methodsMonoclonal antibodies to human factor VII: a one step immunoradiometric assay for VII: Ag T TAKASE, EGD TUDDENHtAM, S CHAND,ALS N H GO DLISON..........................H..........G...............DALL........................337........... 3Monoclonal antibodies to human factor VII: production of immunodepleted plasma for VII: C assayS T TAKASE, EGD TUDDENHtAM,S H ND LSCHANDAL,...................AL..........SON....................G...........DALL...............342...... 4Comparison of two dimensional immunoelectrophoresis and multimer analysis in the study of von Willebrand factor MARGARET AHOWARD, TE,B FR IN..............A.......OATES.....................BG.........F.......RKIN..............346...... 4Letters h E io ........................to............the...............Editor..........................353........ 5Other r sp n e c .............................................................................357.............. 5Book v e s ...........................re..................iew s...............................358............... 5Noiet .............................................e.............................360......................... 6

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ContentsNo 4 APRIL 1988 Page

Survey of prothrombin time in National External Quality Assessment Scheme exercises (1980-87) L POLLER, DA TABERNER,JM T O SO ,K AR Y ..THOMSON,.................................KV............DARBY.......................361........ 6Hodgkin's disease: a flow cytometric study KATE G MORGAN, P QUIRKE, CJ O'BRIEN, CC BIRD.................................. 365Use of morphometry in cytological preparations for diagnosing follicular non-Hodgkin's lymphomas MW STEVENS,KS CROWLEY, NL FAZZALARI, AE WOODS.370......................................................................... 7Dicentric chromosome in the bone marrow of a child with megakaryoblastic leukaemia and Down's syndrome AOM WILKIE,CLARE KITCHEN, A OAKHILL, RT HOWELL, PJ BERRY................................................................... 378Parvovirus as a cause of hydrops fetalis: detection by in situ DNA hybridisation HI PORTER, TY KHONG, MF EVANS, VT-W CHAN,KA F E I G ..................FLEM ING.......................................................381.................. 8Antimicrobial susceptibility testing of Streptococcus pneumoniae: quality assessment results JJS SNELL, RC GEORGE, SF PERRY, YJ ERDMAN. 384Basis of sialic acid heterogeneity in ulcerative colitis JR JASS, K SUGIHARA, SB LOVE......................................... 388Definition of gastroduodenal junction in healthy subjects HH LAWSON.................................................. 393Laboratory assessment of three reflectance meters designed for self monitoring of blood glucose concentrations IF BEGLEY,ARW O R S .......................FORREST...................................................397................ 9Clinicopathological study of myoepithelial sialadenitis and chronic sialadenitis (sialolithiasiS) GM KONDRAT'OWICZ, LESLEY A SMALLMAN,DW M R A ...................MORGAN.....................................................403................... 0Skeletal muscle expression of class II histocompatibility antigens (HLA-DR) in polymyositis and other muscle disorders with aninflammatory infiltrate JAZKIA FECER.........UK.................A.........FLETCHER............................... 410Glycated haemoglobin and glycated protein and glucose concentrations in necropsy blood samples WG JOHN, KWM SCOTT,CR ...F..... .415...

Vasitis nodosa and associated clinical findingS LYNN HIRSCHOWITZ, I RODE, J GUTILLEBAUD, W BOUNDS, EILEEN MOSS................ 419Familial autonomic visceral myopathy with degeneration of muscularis mucosae EM ALSTEAD, MN MURPHY. AM FLANAGAN. AE BISHOP.HIF O G O .......................HOD..........................SON..........................424................ 2Simultaneous acute inflammation in entopic and ectopic pancreaS EW BENBOW........................................... 430Is neutrophil elastase associated with elastic tissue in emphysema? B FOX, TB BULL, A GUZ, E HARRIS, TD TETLEY .................. 435Hepatic tumours induced by anabolic steroids in an athlete TM CREAGH, A RUBIN, DJ EVANS.................................. 441Immunohistological demonstration of progesterone receptor in breast carcinomas: correlation with radioligand binding assays andoestrogen receptor immunohiStology DD GIRI, JR GOEPEL, K ROGERS, ICE UNDERWOOD ...................................... 444Immunohistochemical study of D5 antigen (an oestrogen receptor related protein) in normal breast, benign breast disease, andmammary carcinoma in SitU ANNE GIRLING, M CALEFFI, RIB KING, RR MILLIS.............................................. 448Serum selenium concentration associated with risk of cancer J RINGSTAD, BK JACOBSEN, S TRETLI, Y THOMASSEN................. 454Laboratory techniquesEvaluation of ELISA, RPLA, and Vero cell assays for detecting Clostridium perfringens enterotoxin in faecal specimens PR BERRY,JOANNA C RODHOUSE, STEPHANIE HUGHES, BARBARA A BARTHOLOMIEW, RI GILBERT............................................ 458Evaluation of a rapid method for identifying Branhametla catarrhatis I RICHARDS......................................... 462Electron microscopy of undecalcified human bone RA DODDS, I SHORE, I MOSS ............................................ 465Simple method of estimating severity of pulmonary fibrosis on a numerical scale T ASHCROFT, IUDY M SIMPSON, V TIMBRELL......... 467Immunoturbidimetric assays for serum apolipoproteins Al and B using Cobas Bio centrifugal analyser IN MOUNT, EM KEARNEY,MH O S NE ,BROSSENEU.....................................BM............SLAV..................N........471........ 7Letters e E it r.........................to...........the...............Editor.475................................. 7Other fe p nd n e................correspondence........................................................478......... 7Book v e s ...........................review s................................................479............... 7Notices .480.. 8

No S MAY 1988 Page

Screening of antenatal patients in a multiethnic community for fi thalassaemia trait BARBARA I BAIN .......................... 481A case of IgD myeloma presenting as diffuse osteosclerosis PK MACCALLUM, Al FREEMONT, CG GEARY, el at ...................... 486Tissue type plasminogen activator antigen and activity in sickle cell disease RB FRANCIS JR .................................. 490Amiloride prevents amphotericin B related hypokalaemia in neutropenic patients SR SMITH, MI GALLOWAY, IT REILLY, et at......... 494Histological lesions associated with cyclosporin: incidence and reversibility in one year old kidney transplantS DJ VERSLUIS,FJJW N K TE JWE TNTEN l.........KATE,................G..........WENT.............NG............e..........at........ 498Analysis of chromatin pattern in blood lymphocytes of healthy donors and in lymphoid cells of patients with chronic lymphocyticlekeukaemiaKA WSA IC I,ZTMCZ K ROZYCKA.............W......AW....CK..............TRAC.......YK.....e......at..... 504.0Monoclonal antibody EMB/1 1: high cellular specificity for human macrophages PMA KELLY, EBLISS,JIAMORTON etaa............. 510Diaphragm disease: pathology of disease of the small intestine induced by non-steroidal anti-inflammatory drugs I LANG, AB PRICE,AJ L V ,eIL E V .516..

Comparative study of thc degree of differentiation of neuroblastoma and mean numbers of nucleolar organiser regions M EGAN,F ARAAFAT,, ta ..............................CR.........CKER,..................e...........at.527.............. 2Degree of differentiation in colorectal adenocarcinomas: a multivariate analysis of the influence on survival TB HALVORSEN, F SEIM 532Correlation of urine cytology with ABO(H) antigenicity in transitional cell carcinoma of the bladder G DAS, RW GLASHAN.......... 538Flat in situ carcinoma of the bladder: cytological examination of urine in diagnosis, follow up, and assessment of response tochemotherapy IG M N ...........................WILMA................I......H......GHMAN...............540..... 4Expression of epithelial membrane and 3-fucosyl-N-acetyllactosamine antigens in cervix uteri with particular reference toadenocarcinoma tU P OLASO,inNEsituLLAm,TP aR..LLASON........P...BYRNE,......ANN....WILLIAMS,.........e....at.547..54

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ContentsZinc concentrations in leucocytes of patients receiving antiepileptic drugs WC YUEN, R WHITHOAK, RPH THOMPSON ......... ..................... 553Laboratory techniques

Polybrene technique for red cell antibody screening using microplates JAG LOWN, JG IVEY................................................................... 556Techniques for obtaining differential cell counts from bone marrow trephine biopsy specimens BRIDGET S WILKINS, CJ O'BREN .......... 558Artificial factor VIII deficient plasma: preparation using monoclonal antibodies and its use in one stage coagulation assays VALERIE SHORNSEY, YG WATERSTON, CV PROWSE ...................................................................................................................................... 562

RapidproteinAassay for intrinsic factor and its binding antibody NA SOURIAL .......................................... ........................................ 568Rapid diagnosis of whooping cough using monoclonal antibody PC BORELAND, SH GILLESPIE, LAE ASHWORTH ........... .......................... 573Evaluation of an enzymatic method for determining creatinine in plasma H CROCKER, MDS SHEPHARD, GH WHITE................................ 576Pooling ofsera for human immunodeficiency virus (HIV) testing: an economical method for use in developing countries JC EMMANUEL,M TBASSETT, Hu SMITH, et al................................................................................................. 582Immunocytochemical detection of tumours of neuroectodermal origin P1 CAREY, AJ MALCOLM, ADJ PEARSON, et al ............................. 586Microwave irradiation of human brain tissue: production of microscopic slides within one day ME BOON, E MARANI, PJM ADRIOLO et al.. 590Letters to the Editor ........ ....................................................................................... 594OtherCorrespode and Book reviews............................................................................................... 597

No 6 JUNE 1988 Page

Leading articleClinical interface of blood transfusion AH WATERS, JF DAVIDSON ............................................................................................... 601PapersDiagnostic and prognostic value ofDNA image cytometry in myelodysplasia W AUFFERMANN, INGRID FOHLMEISTER, A B6CKING ........ 604Extramedullary haemopoietic tumours complicating polycythaemia vera PK MACCALLUM, MJ NEWBOULD, PS SAMBROOK, et al............. 609Acquired dysfibrinogenaemia masquerading as disseminated intravascular coagulation in acute pancreatitis JT WILDE, WEG THOMAS,

DA LANE, et al .................................................................................................. 615Late Niemann-Pick disease with neurovisceral storage: a classification problem J MCFARLANE, L MURRAY, K BRADBURY, et al.!............ 619Quantification of oestrogen receptors in breast cancer: radiochemical assay on cytosols and cryostat sections compared with

semiquantitative immunocytochemical analysis HME SCHERES, AFPM DE GOEIJ, MJM ROUSCH, et al........................................................... 623Tumour associated antigens in diagnosis of serous effusions J MEZGER, W PERMANETTER, AL GERBES, et al............................................ 633Antigen profiles in differential diagnosis between colonic and ovarian adenocarcinomas SC HENZEN-LOGMANS, NW SCHIPPER, LG POELS,

et a.................................................................................................. 644C3 and C4 complement components and acute phase proteins in late pregnancy and parturition IZ KOVAR, PAMELA G RICHES ........ ...... 650Immunoblot analysis of immune response to Campylobacter pylori and its clinical associations H VON WULFFEN, Hi GROTE,

S GATERMANN, et al ..................................................................................................... 653Cytomegalovirus infections in heart and heart and lung transplant recipients TG WREGHITT, M HAKIM, JJ GRAY, et al........................... 660Laboratory techniques

Bloodcultures: where do we stand? RC SPENCER................................................................................................................................... 668Automated screening of blood cultures with the Malthus microbiological growth analyser DFJ BROWN, M WARNER, CED TAYLOR, et al. 671

ComparisonofBactec NR-660 and Signal systems KAREN RIMMER, MARGARET CABOT.......................................................................... 676Comparison of conventional and single bottle system for blood cultures JA WILSON, Al BARRATT, J GRAY, et al.................................... 679Evaluation of use of Signal system of blood culture in paediatriCs H FOX, DE HEALING, RH GEORGE....................................................... 683Comparison ofmethods for isolating Mycobacterium avium-intracellulare from blood of patients with AIDS DC SHANSON, MS DRYDEN 687Rapid identification of bacterial antigen in blood cultures and cerebrospinal fluid R GRAHAM WILLIAMS, CA HART............................... 691Evaluation of faecal preservation and staining methods in the diagnosis of acute amoebiasis and giardiasis N SHETTY, T PRABHU ....... 694

Comparison of assays for antibody to HTLVI PHILIPPA MB WHITE .............................................................................................. 700Letters to the Editor ........ ....................................................................................... 703Othercorrespondence and Book reviews............................................................................................... 707

Corrections................................................................................................708

No 7 JULY 1988 Page

Leading articleClinicaldirectorsofpathology: who are they and what do they direct? R DYSON .......................................... ........................................ 709

Review articleHaematological abnormalities in human immunodeficiency virus (HIV) disease CHRISTINE COSTELLO.................................................. 711PapersDetection of ampicillin resistant Haemophilus influenzae in United Kingdom laboratories M POWELL, JD WILLIAMS............................. 716Incidence of decreased penicillin sensitivity of Streptococcus pneumoniae from clinical isolates P NAIR ............. ................................... 720Impairment of cytomegalovirus and host balance in elderly subjects MONICA MUSIANI, MARIALUISA ZERBINI, DANIELA ZAULI, et al.......! 722Neonatal screening for cystic fibrosis in Wales and the West Midlands. 1. Evaluation of immunoreactive trypsin test HC RYLEY,

SM DEAM, WILLIAMS, et al .................................................................................................. 726Significant intimal abnormalities in muscular pulmonary arteries of patients with early obstructive lung disease JUNE M FERNIE,A MCLEAN , D LAMB ...................................................................................................................................... 730

Inflammatory pseudotumour of spleen RFT MCMAHON............................................................................................... 734Smooth muscle pseudotumours: a potentially confusing artefact of rectal biopsy EK DANKWA, JD DAVIES ............ ................................ 737

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ContentsUpper oesophageal gastric heterotopia: a prospective necropsy study in children s vARuEND, Ai HOWAT ........................ 742CD3 + CD8 + T cell lymphocytosis masking B cell leukaemia JL smITH, DG OSCIER, DG HAEGERT, eta! ........................ 746Monoclonal antibody (YI/82A) with specificity towards peripheral blood monocytes and tissue macrophages 'R DAVEY, JACQUELINEL CORDELL, WENDY N ERBER, et a!.753............................................................................ 5Vegetarian diet and cobalamin deficiency: their association with tuberculosis I CHANARIN, ELLA STEPHENSON .................... 759Refractory myelodysplastic anaemias with hypocellular bone marrow Y YOSHMDA, S OGUMA, H ucHINo, et a! ..................... 763Lymphoid aggregates in bone marrow: study of eventual outcome BE FAULKNER-JONES, AJ HOWIE, BJ BOUGHTON, et a! ............. 768Laboratory techusqneACP Broadsheet number 118: Laboratory investigation of paraproteinaemia PAMELA G RICHES, JR HOBBS....................... 776Use of dark ground microscopy in haematology AS TODD, WK BARNETSON............................................... 786In situ hybridisation in formalin fixed, paraffin wax embedded liver specimens: method for detecting human and viral DNA usingbiotinylated probes NV NAOUMOV, GJM ALEXANDERt, ALWF EDDLEsToN, et a!.............................................. 793Method to quantitate intestinal metaplasia of stomach by image analysis CA RUBIO, A PORWIT, M RODENSJd5..................... 799Rapid indirect enzyme linked immunosorbent assay (ELISA) for detecting antitoxoplasma IgG: comparison with dye test JANET HFRANCIS, A NE HMJY SO ...........RA........PAYNE,..................DHM..........JOYNS...............N......802....0New rapid latex agglutination test for diagnosing Trichomonas vaginalis infection JA CARNEY, P uNADKAT, A YuLE, et a!............ 806Letrt t h Etters......................to............the.............................tor...............809........ 0Boo r v e s .............................e............................................814....................... 1Noteices ........................................................................816......................... 1

No 8 AUGUST 1988 Page

Leading artideMeasurement of pathology workload in the United Kingdom I CAVILL................................................. 817Occasional artkideThe confidential enquiry into maternal deaths: its role and importance for pathologists I DAWSON ............................ 820PapersDifferences in lipopolysaccharide profiles of serologically identical Legionella pneumophila serogroup 6 strains OG ZANEN-LIM,HC Z N N ................................ANEN.........................................826...................... 2Post mortem radiology in children with congenital heart diseas GA RUSSELL, P1 BERLRY..................................... 830Histological analysis of parotid and submandibular glands in chronic alcohol abuse: a necropsy study J SCOTT, J BURNS, EA FLOWER. 837Representative sample of rheumatoid synovium: a morphometric study TD KENNEDY, C PLATER-ZYBERK, TA PARTRIDGE, et a!........ 841Morphometric comparison of synovium from patients with osteoarthritis and rheumatoid arthritis TD KENNEDY, C PLATER-ZYBERK,TA P R RM E tal....PARTRIDGE,............................................e.............a!.............847......... 4Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cellS KAREN AF PULFORD, WENDY NERBER, A CI C JULIE................................A.......CRICK,...................et.........a..........853...... 5Use ofimmunohistochemical methods in the differential diagnosis between primary cerebellar haemangioblastoma and metastatic renalcaciorc D OUDnoRUHmaEBELDRODO.....UL.....ESBROUGH.................E....BELL,.........A...GORDON........... 861.6Prognostic indicators in centroblastic-centrocytic lymphoma NR GRIFFIN, MR HOWARD, P QUIRKE, et a! ......................... 866Silver deposition in the cervix after application of silver nitrate as a cauterising agent DG LOWE, DA LEVISON, PR cRocKER, et a!...... 871Quantitation of human milk fat globule (HMFGI) expression in breast carcinoma and its association with survival DM PARHAM,W LD ER,ASLB R SOIDDERS............................Al..........ROBERTSON...............................875....... 7Microvillus inclusion disease: specific diagnostic features shown by alkaline phosphatase histochemistry BD LAKE ................ 880Myeloproliferative disease in children: a demographic study MM REID, PwG SAUNDERS, J KERNAHAN ........................... 883Slow and incomplete histological and functional recovery in adult gluten sensitive enteropathy JMM GREFTE, JG BOUMAN, J GROND, eta! 886Laboratory techniqucaGlycol methacrylate (GMA) embedding for light microscopy. II Immunohistochemical analysis of semithin sections of undecalcifiedmarrow cores LM,EA CIMAU, S HEDISLAM, l ....E...ARCH......MBAUD.........ES....HENDERSON,............et....a!.... 892.89Non-isotopic detection of in situ nucleic acid in cervix: an update protocol J BURNS, AK GRAHAM, JO'D MCGEE................... 897Definitive identification of human T cells in formalin fixed paraffin wax embeded tissue ND FRANCIS, DM CLARK, AW BOYLSTON .... 900Evaluation of six screening methods for detecting significant bacteriuria TK SMITH, ANDREA J HUDSON, RC SPENCER................ 904Evaluation of the Microbact-24E bacterial identification systeM JULIA M LING, Y-W HUI, GL FRENCH........................... 910Use of theophylline in the investigation of pseudothrombocytopenia induced by edetic acid (EDTA-2K) 0 OHNUMA, Y SHIRATA,K I A A A..........M...............YA.....................AW A............................915.................. 1Microtitre plate method for alanine transferase (ALT) assayS AC ARCHER, DF GREEN, EJ BUCKLAND............................ 918tatters eE it r.........................to...........the...............Editor.920................................. 2Otherrrscorrespondence.........................................and.........Book.................view......... 922Correct ........................................................on......................924.................. 2Notices .........................................................................924......................... 2

No 9 SEPTEMBER 1988 Page

PapersDiagnostic ipen otsdeequipmentyoutsideA YFE ........the....laboratory..........JM....B....R..........A.......... 92592Serum concentrations of laminin P1 in diabetics with advanced nephropathy P PIETSCHMANN, G SCHERNTHANER, CM SCHNACK, et al.. 929Effects ofimproved glycaemic control on calcium and magnesium homeostasis in type II diabetes AM MCBAIN, IRF BROWN, DO MENZMES,eta! .................................................................933.................................... 3

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ContentsScreening tests for glycosaminoglycans in urine: experience from regional interlaboratory surveys JM RATrENBURY, E WORTHY, JOYCE CALLEN ....................................................................936............................... 3Association between coagulation factors VII and X with triglyceride rich lipoproteins J CARVALHO DE SOUSA, CLAUDINE SORIA, MARISEAYRLAU RIRLT-JARR.........................................ER...............e............a!.940.................. 4Influence of heat treatment on FVIII:C recovery from freeze dried cryoprecipitate AG BENNY, PA OCKELFORD, As JOHNs, et al!........ 945Seronegative blood products prevent primary cytomegalovirus infection after bone marrow transplantation S MACKINNON,AKB RN T ,BURNETr,a .....................RJ........CRAWFOR....................,.....e.........a!.948............ 4Morphology and immunology of circulating cells in leukaemic phase of follicular lymphoma JUNIA V MELO, DANEELE SF ROBINSON,MARIA D LiP e l. ..............DE.........OL..........VEIRA.....................et..........a!.........951...... 5Sea-blue histocytes and Gaucher cells in bone marrow of patients with chronic myeloid leukaemia PR KELSEY, CG GEARY........... 960Mac 387 antibody and detection of formalin resistant myelomonocytic LI antigen P BRANDTZAEG, DB JONES, DJ FLAVELL, et a!...... 963Percutaneous renal biopsy specimnens in stone formers DJ HARRISN, JA INGLIS, DA TOLLEY .................................. 971Humoral reaction in the inflamed colon in Hirschsprung's diseas and ulcerative COlitiS GR ARMSTRONG, F RAAFAT................ 975Susceptibility testing of Neisseria gonorrhoeae to penicillin and spectinomycin in a diagnostic laboratory MO GILL, CJ ISON.......... 978Monoclonal antibodies show Listeria monocytogenes in necropsy tissue samples J MCLAUCHLIN, A BLACK, HT GREEN, et a!.......... 983Should we still be doing duplicate immunoassays? EVA LESTER, CATHRYN CORNS .......................................... 989Caecal perforation in a renal transplant patient with disseminate histoplasmosis MARY T BRETT, JTC KWAN, MRt BENDING.......... 992Laboratory techniquesACPBradhBroadsheet tetstodee119:soinSimple.......tests.....to....detect.......poisoning.........a..WIDDOP....... 99699Detection ofcytomegalovirus by in situ hybridisation and immunohistochemistry using new monoclonal antibody CCH2: a comparisonofmehoSmethodsEKTUGFNN HHNBS,EDOBI.......EK......TERESA.........FINN,.......H...H...RB....T.....et....al.... 1005.10Rapid conventional scheme for biochemical identification of antibiotic resistant enterobacteriaceae isolates from urine JD PERRY,M O D,N H FORD,l.................N......H.......ERSING........................et..........a!.........1010......... 1 1Marking planes of surgical excision on breast biopsy specimens: use of artists' pigments suspended in acetone DA PATERSON, JD DAVIES. 1013Method for showing human spermatogenesis using Arachis hypogaea (AHA) lectin CAROLYN JP JONES, EW BENBOW, W FIONA KNOX, eta!.1017..................................................................................................... 1 1Rapid eho freamethodiato JNCfor .....fetal........brain........fixation............JM....NICHOLLS............ 1019.11Simnple procedure for assessing relative quantities of neutral and acidic sugars in mucin glycoproteins: its use in assessing cyclical changesin cervical Cmuc, E EI, B LEEN,ns l.CB....G...LK........PE....REID,.......PB....CLEMENT,..........e.....a!.... 1021.02Letters h E io .......................to...........the...............Editor.........................1025........... 1 2Othercorrespondenceies........................................and.........Book...........re.......ie.......s...1028Noieot ...............................ces..................................1031............................... 1 3

No 10 OCTOBER 1988 Page

Occasional articleStatistics on microcomputers. A non-algebraic guide to their appropriate use in biomedical research and pathology practice. I Datahandling dprlimnayandysS RpreliminaryN EC ......analysis.........RA....BROWN,...........SWANSON........BECK..... 103303PapersNon-invasive investigation of liver disease in haemophilic patientS ELIZABETH J MILLER, CHRISTINE A LEE, P KARAYIANNIs, et a!.......1039Nucleolar organiser regions in normal, cirrhotic, and carcinomatous livers J CRocKER, JULIE mCGOVERN........................1044Beta-2-microgiobulin expression in the liver after liver transplantation SG HUBSCHER, DH ADAMS, E ELIAS........................1049Immunochemistry and electron mlicroscopy of head and neck rhabdomyoma TR HELLIWVELL, MCI SISSONS, PJ sTroNEY, et a!..........1058Tumour marker immunoreactivity in adenocarcinoma 10 ELLIS, A HITCHCOCK............................................1064Influence of mucinous components on survival in colorectal adenocarcinomas: a multivariate analysis TB HALVORSEN, EvA sEIM .... 1068Primary malignant melanoma of the gall bladder DI HEATH, C WOMAK..................................................1073DNA content and MHC class II antigen expression in malignant melanoma: clinical course J ZALOUDIK, H MOORE, AK GHOSH, et aL! 1078Intraplacental choriocarcinoma: a report of two caseS H FOX, RN LAURINI...............................................1085Urinary excretion of albumin and retinol-binding protein during normal pregnancy R BEETHAM, A DAWNAY, H MENABAWY, et a!.....1089Campylobacter-like organisms and Candida in peptic ulcers and similar lesions of the upper gastrointestinal tract: a study of 247CaSeS NK KALOGEROPOULOS, R WHITEHEAD.......................................................................1093Cerebral infection with Escherichia coli 0157:H7 in humans and gnotobiotic piglets S TZIPORI, CW CHOW, HR POWELL .............1099Shedding of oocysts of Cryptosporidiwn in immunocompetent patientS R CAMERON SHEPHERD, CAROLE L REED, GP SINHA...........1104Plasma oxolate concentration and secondary oxalosis in patients with chronic renal failure CRV TOMSON, SM CHANNON, IS PARKINSON, etal..............................................1107........................................................ 1 0Low serum haemolytic function of the fourth complement component (C4) in insulin dependent diabeteS G SENALDI, BA MILLWARD,mS H SANO eH.................ss...............N...............et..............a!..............1114............... 1 1Lactoferrin in aspirates of odotogennc cyst fluid AJ SaMIT, JB MATTrHEWS, GI MASON, et a! ....................................1117Laboratory techniquesAssessment ofDNA content and cell cycle distribution oferythroid and myeloid cells from bone marrow J HoDGETr, TG HOY, A JACOBS. 1120Extraction ofDNA from small sections of frozen tissue with simultaneous histological examination FE COfTER, PA HALL, BD YOUNG. 1125Screening rbateiuiaforCintbacteriuria, KNswith.........C...initec..............MM...D...RAN......J..KEN.......... 112712An ELISA to detect antipseudomonal IgA antibodies in sera of patients with cystic fibrosis MM BRETr, ATM GHONEIM, JM LITTLEWOOD 1130Letretttert r............................othe...................................or.............1135............. 1 3Otherrrsocorrespondewe...............................ce.......and.........Book...........reviews.............. 1138

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ContentsNo 11 NOVEMBER 1988 Page

Occasional articlesPresidential Address: Crystal ball gazing and the effects of fate GW PENNINGTON..........................................1141Statistics on microcomputers. 2 Confidence intervals and significance testS RA BROWN, J SWANSON BECK........................1148PapersMixed lymphocyte reactions do not predict severity of graft versus host disease (GVHD) in HLA-DR compatible, sibling bone marrowtransplants SH LIM, WN PATTON, S JOBSON, et alt .................................................................. 1155In vivo oxidation of the methyl group of hepatic 5-methyltetrahydrofolate M LUMB, I CHANARIN, R DEACON, et alt.................1158Leukotriene B4 synthesis and neutrophil chemotaxis in chronic granulocytic leukaemia IRENE AG REILLY, HR KNAPP, GA FITZGERALD. 1163Red cell hypoplasia, thrombocytosis, and leucocytosis: myelodysplastic and proliferative syndrome A CRAIG, CG GEARY, EM LOVE, et at. 1168Demonstration of aluminium in iliac bone: correlation between aluminon and solochrome azurine staining techniques with data onflameless absorption spectrophotometry HA ELLIS, MMC PANG, WHB MAWHINNEY, et at ......................................1171Measurement of protein HC (Li, microglobulin) and protein HC-IgA complex in different body fluids JL FERNANDEZ-LUNA, F LEYVA-COBIAN MNE,.................................E..........M.........N..........E...............1176............... 1 7Brush cytology of the colon and rectum in ulcerative colitis: an aid to cancer diagnosiS OH MELVILLE, PI RICHMAN, NA SHEPHERD, et at. 1180Use of endoscopic biopsy for diagnosing congestive gastropathy CM CORBISHLEY, SM SAVERYMUTTU, JO MAXWELL.................1187HMFG-2 as a prognostic indicator in superficial bladder cancer IG CONN, J CROCKER, LA EMTAGE, et at.........................1191Anatomical variations in human carotid bodies Q KHAN, D HEATH, P SMITH ..............................................1196Nucleolar organising regions in cervical intraepithelial neoplasia DC ROWLANDS ............. ............................1200Laboratory techniquesGuidelines on selection of laboratory tests for monitoring the acute phase response International Committee for Standardization inHaematology (Expert Panel on Blood Rheology) ...................................................................1203Automated measurement of plasma viscosity by capillary viscometer BM COOKE, J STUART ..................................1213Sensitive avidin biotin based technique for identifying liver membrane antigens in tissue sections J SIPOS, BM MCFARLANE, R WILLIAms, etal. .1 17.. 1 1Evaluation of five commercial kits to detect dsDNA antibodies SA MCMILLAN, ANNE C FAY ..................................1223Clinical laboratory environmental contamination: use of a fluorescence/bacterial tracer DA KENNEDY, JF STEVENS, AN HORN.........1229Enzyme linked immunosorbent assay (ELISA) for cytomegalovirus antibody in donor plasma S RAMSKILL, P SENIOR, P BARLOW, et al. 1233Aceurate automated leucocyte differential counts despite profound leucopenia SALLY E KINsEY, MJ WATTS ......................1236TOXBASE: a microcomputer database for post mortem toxiCOlogy K ROBINSON, I MARSH, ARW FORREST .......................1240Letters h E io .......................to...........the...................ditor......................1242........... 1 4Other correspondence and Book reviews..........................................................................1247

No 12 DECEMBER 1988 Page

Occasional articlesReview: Mechanism of action of intravenous immunoglobulin in immune-mediated cytopeniaS HI ATRAH, RJL DAVIDSON...........1249Statistics on microcomputers. A non-algebraic guide to their appropriate use in biomedical research and pathology practice. 3 Analysisof variance and distribution-free methodS RA BROWN,]J SWANSON BECK .................................................1256PapersSerum amyloid A protein, apolipoprotein A-I, and apolipoprotein B during the course of acute myocardial infarction CPJ MAURY,KJT TE M N,C G TFOTTERMAN,..........................C-G..........GREF,................et........at........1263....... 1 6Vitronectin (serum spreading factor): its localisation in normal and fibrotic tissue JT REILLY, JRG NASH.........................1269Feasibility of new prognostic classification for rectal cancer SAC DUJNDAS, RW LAING, A O'CATHAIN, et at ........................1273Clinicopathological importance of primary dysplasia of bladder RJ ZUK, HS ROGERS, JE MARTIN, et at ..........................1277Use of histological examination to assess ultrastructure of liver in patients with long standing jejuno-ileal bypass for morbid obesityAP BOON, H THOMPSON, RM BADDELEY.1281.......................................................................... 1 8Immunohistochemistry of cytokeratin proteins in squamous and transitional cell lesions of the urinary tract MF TUNGEKAR, KC GATTER,MS A D A I ............AL-A.............................NAN.............................1288.................... 1 8Immunohistochemical study of 22 cases of thymoma 0 LEE, OH WRIGHT ................................................1297EBM/ I I reactivity in malignant histiocytosis PMA KELLY, M MCGOVERN, KC GATTER, et at ...................................1305Human herpes virus type 6 (HHV-6) and its in vitro effect on human immunodeficiency virus (HIV) GR PIETROBONI, GB HARNETT,TJF R,etaJ ........FARR,...............................et................at.1310................................. 1 1Retrospective study of Campylobacter-like organisms in patients undergoing partial gastrectomy RJLF LOFFELD, BCAJ LOFFELD, JWARNSEND l..............................,............et.................at................1313................... 1 1Campytobacter pyloni:clinical, histological, and serological studies C MUSGROVE, FJ BOLTON, AM KRYPCZYK, etaa.................1316Laboratory techniquesGuidelines on platelet function testing THE BRITISH SOCIETY FOR HAEMATOLOGY BCSH HAEMOSTASIS AND THROMBOSIS TASK FORCE......1322Comparison of latex and haemolysin tests for determination of anti-streptolysin 0 (ASO) antibodieS GDW CURTIS, WILMA AG KRAAK,RGM THLITCHELL ....................................................................1331....................... 1 3Diagnosis of nasopharyngeal tuberculosis by detection of tuberculostearic acid in formalin fixed, paraffin wax embedded tissue biopsyspecimens NOD,C CAN W HENGMt lARNOLD,............CY......CHAN,.........SW......CHEUNG,............et......at... 1334Letters h E io .......................to...........the........................itor..................1337........... 1 3Litoist s o s ...............of...................ssessors.......................................1340.............. 1 4Annual author index to Volume 41.1341............................................................................ 1 4Annual subject index to Volume 41.1346............................................................................ 1 4Annual contents index to Volume 41.

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