Consultant Submittal Guidelines for the Upgrade/Renovate

Upgrade/Renovate/Expand Research Labs (Biosafety Lab Renovation – Health Sciences Research Building) University of Kentucky

Project Number 2573.0

Consultant Submittal Guidelines & Executive Summary Page 1 of 7

Consultant Submittal Guidelines for the

Upgrade/Renovate/Expand Research Labs (Biosafety Lab Renovation – Health Sciences Research Building) for the

UNIVERSITY OF KENTUCKY PROJECT NO. 2570.0 PROJECT SCOPE: $1,500,000

The University of Kentucky is requesting the services of an ARCHITECTURAL/ENGINEERING TEAM to provide design services for the Upgrade/Renovate/Expand Research Labs (Biosafety Lab Renovation – Health Science Research Building), located in Lexington, Kentucky. This solicitation seeks a team that will provide and be responsible for architectural, M/E/P, structural, and specialty lab (as needed) design services. The team will also be responsible for bidding and construction administration services required to complete the project.

The team should be led by a local or regional architectural firm that will serve as prime architect having the responsibilities of the architect of record and bear the responsibility to schedule the design process; coordinate the early stages of the design; oversee the production of drawings and bid documents; provide bid phase services; obtain plan approvals from AHJ; provide construction administration and all other day to day management and oversight of the design and construction process.

The team should include additional sub consultants that are required to produce comprehensive design and bidding documents, effective construction phase administration, and a final product of the highest standard. A contract will be negotiated for the full design, bidding and construction services. In addition, an allowance will be negotiated as a reimbursable service to provide Independent Testing Services (to include special inspections), air flow and balancing study, and a security and hardware consultant. The consultant shall be responsible for review of all reports and for writing any final acceptance letters as may be required by authorities having jurisdiction.

Hereinafter are listed the criteria, including the scoring weights, by which each firm’s submittal will be evaluated. Bearing this in mind, each firm's submittal should clearly and thoroughly address all criteria to allow objective evaluation of the firm's previous experience and capability to successfully complete this project. All submittals should be in an 8 1/2" x 11" bound format and GIVE SPECIFIC PROJECT EXAMPLES, including photographs, drawings, resumes, programs, etc. to properly substantiate the firm as well as individual experience in all categories.

The submittal should include a BRIEF EXECUTIVE SUMMARY (maximum of 3 pages) as a cover to the submittal, as well as a response to each of the evaluation criterion (maximum of one page per criterion). The SF 330 should be provided in support of the Executive summary and criteria response. BE SURE TO




SPECIFY WHO THE PROJECT MANAGER WILL BE IN THE EXECUTIVE SUMMARY. The Project Manager is defined as the consulting team member who will act as the Design Team’s primary point of contact for the duration of the project with the University’s designated Project Manager to coordinate and manage design and construction administration services and/or activities.

The consultant should focus their submittal on the issues and needs that are unique to this project. Design Teams expressing interest in this project should demonstrate what special experience and/or attributes their team has that makes them the most qualified for this project.

Include ONLY relevant projects that have been completed within the last ten (10) years for the overall team, individual firms and the Project Manager. Use specific project examples that will illustrate the team and individual team members’ abilities to complete this project in a satisfactory manner.

The University of Kentucky is dedicated to promoting minority sub-consulting firms’ participation in university work. Consultants are encouraged to consider using the services of minority sub-consulting firms where the demands of the project will permit.

(Scoring for each category will be as indicated herein.) EVALUATION CRITERIA The following criteria will be used in the evaluation of the submittals using an overall 100-point scale:

I. PRIMARY FIRM (35 Points) Primary firm’s recent experience involving Biosafety Laboratories (BSL3) renovations within an

operating medical research center. The firm shall demonstrate an ability to coordinate design team members and detail methods that will be used to ensure that the design schedule is met, including milestone and productivity monitoring.

The firm’s strategy to assure –

Compliance with the defined programmatic needs. A well-designed and coordinated construction package. An effective construction administration process. An exceptional final product for a facility of this scale and complexity.

Special consideration will be given to experience with BSL3 laboratories within an operating medical research center.

II. OTHER DESIGN TEAM MEMBERS (20 Points)

Emphasis should be placed on indicating who the design team will be as well as their availability for this project. Attention should be given toward indicating who the sub-consultants will be, their




respective rolls, and who the specific team members will be. Skills necessary to meet the project schedule, budget and design criteria should be demonstrated by all team members.

Identify key personnel within the firm and their corresponding roles on this project. Explain their qualifications resulting in their being proposed for this project.

Include a matrix indicating who the full design team members will be and what their respective

roles were with the examples listed.

III. PROJECT MANAGER’S QUALIFICATIONS (25 Points)

Identify who the Project Manager will be and indicate his/her experience with planning,

managing and coordinating all aspects of a project of similar complexity in a professionally competent manner. Experience should include management and coordination of a full design team on projects of similar scope and complexity. Project Manager should have recent experience with additions to and renovations of existing university facilities and/or other projects of similar scale and complexity; submittal should explain the significance of each project listed. (Please include a maximum of 5 project examples.)

Design Team should be experienced in working with current technological tools, to include

designing using building information modeling (BIM) and web-based project management systems.

Design Team should show commitment necessary to adequately manage and coordinate

the project through all phases of programming, design, contract documents, bidding and construction administration – maximizing project funds, while minimizing change orders and maintaining an aggressive project schedule.

IV CONSTRUCTION ADMINISTRATION (20 Points)

Explain Team’s approach to construction administration, and how the Team will respond to issues that may arise during construction.

Design Team should be familiar with the CM delivery method and should have experience working with Construction Management firms for coordination and completion of projects using this delivery method.




The consultant should be careful to address each criterion, as neglect of any section will result in a lower total score for the firm. DO NOT ASSUME THOSE REVIEWING THE SUBMITTALS ARE ALREADY FAMILIAR WITH YOUR FIRM. Your presentation should be concise and to the point. Emphasis should be given to a few examples, which clearly show the team’s qualifications, rather than numerous examples, which are unrelated to the project. For further information concerning the scope of this project, contact Sandy Redmon, Project Manager, at (859) 218-3115.

Please use the “Submit Proposal” button next to the project listed on CPMD’s website to submit your documents electronically in pdf format.

In addition to the electronic copy, please provide eight (8) bound copies of the submittal for

review. Submit to:

Capital Project Management Division

222 Peterson Building 411 South Limestone Street Lexington, KY 40506-0005

ATTN: Sandy Redmon

SUBMITTALS MUST BE RECEIVED NOT LATER THAN 3:00 PM EST, ON MONDAY, OCTOBER 4, 2021.

Submittals received after this time will not be reviewed.




Executive Summary B. PROJECT DESCRIPTION

The University of Kentucky is home to leaders in the fields of immunology, microbiology, and infectious

diseases. As the flagship university of the state of Kentucky, the university is often called upon to respond to health emergencies. In response to the COVID-19 pandemic, the University of Kentucky is embarking on an ambitious project to expand it research portfolio on emerging and re-emerging pathogens. To support these research activities, the university will renovate an existing aging BSL-3 facility that was originally constructed in 2000.

This project will renovate the 1,450 square foot BSL3 laboratory that currently consists of eight (8) spaces:

2 animal holding rooms, 2 procedure rooms, common lab spaces, an anteroom, 2 locker/showers and the common lab space. The renovation should accommodate an animal procedure room in addition to a large, shared laboratory. Following is a partial list of deficiencies identified by Cornerstone Commissioning LLC as needing correction:

- Replace current autoclave - Address leaks in containment barrier - Install relative humidity sensors - Ensure review of temperature sensors - Make corrections to room pressure monitors - Adjust room airflow to achieve desired room pressures - Adjust room differential set up - Make necessary repairs on isolation dampers, supply, and exhaust - Make necessary corrections to isolation damper control pad - Evaluate exhaust fan - Obtain all required certifications

See attached final commissioning report for full details.

The design will consist of preparation of Phase 1-2 and 3 documents in accordance with University and Medical Center standards and procedures. A conceptual program and preliminary opinion of probable construction and project cost has been developed and will be released to the shortlisted teams.




C. PRELIMINARY PROJECT BUDGET

TOTAL CONSTRUCTION BUDGET* $925,000

TOTAL PROJECT SCOPE $1,500,000

* The Consultant's Phase 1, 2 & 3 cost estimate submittals for the project are not to exceed this specified amount. Budget compatibility is the responsibility of the Consultant and design of the project beyond the available construction dollars listed above is unacceptable.




D. PRELIMINARY PROJECT SCHEDULE The following is the tentative schedule presently proposed for this project:

Monday, September 20, 2021 Project Advertised for Consultant

Monday, October 4, 2021 Consultant Submittals Due

Friday, October 8, 2021 Short List Meeting

Friday, October 22, 2021 Design Team Interviews

Monday, November 1, 2021 Design Team Contract Negotiated

Friday, November 5, 2021 Program Meeting

Wednesday, November 17, 2021

Program Review

Monday, November 22, 2021 Begin Phase 1-2 Design

Monday, January 31, 2022 Phase 1-2 Documents Submitted to CPMD

Monday, February 14, 2022 Phase 1-2 Review

Monday, March 21, 2022 Phase 3 Documents Submitted to CPMD

Monday, April 4, 2022 Phase 3 Review Meeting

Monday, April 18, 2022 Final Documents Submitted to CPMD

Monday, May 2, 2022 Advertise for Bidding

Thursday, May 12, 2022 Pre-Bid Conference

Thursday, June 2, 2022 Bid Opening

Friday, June 10, 2022 Contract Awarded

Monday, June 20, 2022 Construction Begins

Thursday, November 10, 2022 Substantial Completion

Saturday, December 10, 2022 Final Completion

Friday, November 10., 2023 Warranty Expires

11 Cold Spring Drive, Boxford, MA 01921

Tel 978-887-8177 | Fax: 978-359-7775

www.CCxcx.com

PERFORMANCE VERIFICATION REPORT

UNIVERSITY OF KENTUCKY PETER BOSOMWORTH HEALTH SCIENCES RESEARCH BUILDING (HSRB) FOURTH FLOOR BSL-3 LABORATORY LEXINGTON, KY AUGUST 10, 2021 – REV. 0

PO Box 156, Boxford, MA 01921

Tel 978-887-8177 | Fax: 978-359-7775

www.cornerstonecx.com

ESTABLISHING A FOUNDATION

FOR PERFORMANCE

http://www.cornerstonecx.com

http://www.cornerstonecx.com/

Univ of Kentucky – HSRB – 4th floor BSL-3

PV Report - 2021


Table of Contents

1. Executive Summary

2. Annual Performance Verification Plan

3. Corrective Action Plan

4. Facility Performance Tests and Data

5. Integrated Systems Testing (Failure Scenario Testing)

6. Supporting Documents

www.CCxcx.com


PV Report - 2021


Date: August 10, 2021 To: Brandy Nelson

Biosafety Officer University of Kentucky – Lexington [email protected] 859-550-0333

Subject: Performance Verification for the University of Kentucky – HSRB – Fourth floor BSL-3 Laboratory EXECUTIVE SUMMARY

Cornerstone Commissioning (CCx) has completed the performance verification (PV) of the UK HSRB fourth floor BSL-3 Laboratory located in Lexington, KY. Based on our observations, teleconferences and correspondence, Cornerstone Cx understands that UK has plans to renovate the BSL-3 laboratory for a new research team. The Corrective Action Plan (CAP) lists all items noted as part of the performance verification process that were discovered and need to be addressed. The CAP has recommended actions to take and how the items will be or were resolved. We would like to point out that some of these items may be addressed and not be a concern as part of this renovation. We welcome the opportunity to review all of the items and assist UK to support the important planned research in this BSL-3 laboratory.

The following summary details the items that were verified and documented as part of the performance verification process. Any systems or equipment that is not functioning is documented in the CAP:

1. Bio Safety Cabinets (BSL-3 primary containment):

a. There are five (5) Class II Type A2 recirculating BSCs; one (1) Class II Type B2 BSC.

b. BSC certification has expired for all six (6) BSCs.

2. HEPA filtered exhaust:

a. Multiple bag-in/bag-out HEPA housings located on the roof, which serve the BSL-3 laboratory exhaust air terminal units/ fans.

b. HEPA certification has expired.

3. Decontamination system – Autoclaves: Primary means of decontamination of all laboratory waste is the autoclave. The autoclave is not operational. There is no biological indicators (BI) logbook with current results.

4. Room differential pressure monitors were verified, and values were documented to confirm accuracy of both the local displays outside the laboratory and building automation system.

5. Relative room pressures - Throughout testing CCx confirmed that the directional airflows are maintained at the gradients acceptable to UK and industry standards throughout the test period.

6. Room integrity verification (BSL-3 secondary containment barrier)

a. CCx used a smoke generating device to verify the integrity of the secondary containment barrier. The room integrity tests were conducted at normal room pressures.

b. There were a small number of leaks discovered in the facility. All items identified need to be repaired by UK staff or contract team. If a layout change is made, suggest addressing the items on the remaining existing walls, ceilings, and floors as applicable.

7. Temperature – Verified that the room temperature sensors are reading correctly, and that the control system can meet the setpoint. We were not able to review trend data from the BAS to verify that the temperature control system is working correctly in all laboratory spaces.

8. Relative humidity – Verified that the room RH values are within acceptable values for anticipated research. There are no RH sensors installed, as such, we could not review any trend data from the BAS to verify that the RH remains acceptable in the spaces through all seasons in all laboratory spaces. Animal holding rooms could benefit from the addition of these sensors at a minimum.

mailto:[email protected]


PV Report - 2021


9. All sinks were exercised as part of this performance verification process. Hot and cold water were available at all locations. The electronic eye batteries need to be replaced in most of the hand sinks. Emergency showers and eye washers were also verified as operational.

10. Electrical outlets, lighting control, light fixtures and bulbs were verified operational.

11. CCx confirmed that all the doors close and latch by themselves. The door hardware was confirmed to be in good working order.

12. CCx confirmed all access control, including the card readers and door interlocks function. All security related items were confirmed as operational.

13. CCx tested the supply and exhaust isolation dampers for functionality, as applicable.

14. CCx documented the room airflows, ensuring that all supply and exhaust Phoenix Controls air valves are in control, and that they provide steady values for steady room differential pressure. These were compared to the original design and testing and balancing (TAB) report. Any modifications or changes to setpoint are noted in the applicable section.

15. CCx confirmed the air change rate in the BSL-3 spaces.

16. Air Handling Unit Supply Fans serving the BSL-3 – Inspected the general condition of the AHU; belts, filters, cleanliness, etc. with no issues noted. Maintenance is performed according to manufacturer’s recommendations for greasing of bearings, filter change out, etc. Visual inspection shows no issues or concerns.

17. Exhaust Fans serving the BSL-3 – Inspected the belts, no issues noted. Maintenance has not been performed according to manufacturer’s recommendations. Visual inspection shows a potential issue with EF-1 bearings.

18. Integrated Systems Testing (IST) – Failure Scenario Testing – CCx performed failure testing with UK staff and for the BSL-3 equipment and spaces:

a. Air Handling Unit Supply Fans - CCx performed failure testing of the redundant supply fans, the test results with the room differential pressure graphs are detailed in IST#1.

b. Exhaust Fans - CCx performed failure testing of the redundant exhaust fans, the test results with the room differential pressure graphs are detailed in IST#2.

c. Power failure test – CCx performed electrical power failure testing. The test results with the room differential pressure graphs are detailed in IST#3.

d. No reversal of airflows were noted at the secondary containment barrier during these failure test scenarios.

Supporting details for all observations are included in the applicable sections or as attachments with this report.

Refer to the Facility Performance Test and Data section for details, measurements, and readings.

If there are any questions, concerns, or comments, please feel free to contact me at any time.

Respectfully Submitted,

Daniel Cook | LEED AP® | Director of Critical Projects Cellular: 603.944.1633 | Email: [email protected] CC: Dan Frasier, PE, CCP | Founder / CEO; Cornerstone Commissioning, Inc.



PV Report - 2021


Performance Verification Plan

Performance Verification Plan Project #: 1929

Rev # Issue Date Issued By / Initials Revision Description

1 2021-07-08 Cornerstone Cx / DC Initial Draft

2 2021-07-19 Cornerstone Cx / DC Rev 1

3 2021-07-27 Cornerstone Cx / DC Rev 2, FM & BSO Q, BMBL

The Performance Verification (PV) Plan is a document that may be revised over the course of the PV. The revision control

tracks current and past revisions to ensure the most updated plan is being used.

Facility Information

Owner / Entity / Client: University of Kentucky (UK)

Building / Facility: Health Sciences Research Building (HSRB)

The ABSL-3 laboratory is 1,450 square foot facility consisting of eight (8) spaces; 2 animal holding rooms, 2 procedure rooms,

common lab spaces, and an anteroom that has doors to the public corridor, the 2 locker / showers and the common lab

space.

The HVAC system consists of an air handling unit, 2 exhaust fans, and a HEPA filter bag in/bag out housing serving each

exhaust fan. There are Phoenix Controls air valves installed on the supply and exhaust for the labs. The supply has a reheat

coil. There are also isolation dampers on each supply and exhuast duct for isolation during decontamination, these are

controlled via a switch inside a panel in the ante room.

Address: 1095 Veterans Dr., Lexington, KY, 40508

Laboratory Description

Laboratory location (floor): Fourth floor

Year built: Renovation in 2000

The Performance Verification (PV) process has been developed by Cornerstone Commissioning (Cx) to ensure that the

systems, equipment and administrative controls meet or exceed the requirements and recommendations from industry

guidelines, best practices, owner and or consultant recommendations, that are specific to the laboratory program and

facility.

The PV process satisfies the industry requirement to re-verify and document the design, operational parameters, and

procedures at least annually of the laboratory.

Performance Verification (PV) Process Overview

Industry Guidelines for PV

PV Plan Purpose

The PV plan describes how Cornerstone Cx will execute and document this process based on the scope of work in the PV

proposal. The PV schedule of activities is listed in the order which they should be performed, with some activities occurring

off site before and after the onsite testing and observations.

PV Plan Document Revision Control

Document Control

Biosafety in Microbiological and Biomedical Laboratories (BMBL) - 6th edition - June 2020

Section IV—Laboratory Biosafety Level Criteria | Biosafety Level 3 | D. Laboratory Facilities (Secondary Barriers):

Page 48 - Biosafety Level 3, D. Laboratory Facilities (Secondary Barriers), 15. The BSL-3 facility design, operational

parameters, and procedures are verified and documented prior to operation. Facilities are tested annually or after significant

modification to ensure operational parameters are met. Verification criteria are modified as necessary by operational

experience.


Performance Verification Plan Univ of Kentucky

HSRB 4th floor BSL-3

Lexington, KY

Floorplan for reference (drawing):




Lexington, KY

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Thu

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-26

20

21

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-27

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-29

EH&S /Lab

Staff

Facilities /BAS

Access

1Review any changes or adjustments to systems serving the lab since last Performance

Verification or initial commissioning.X X X

2Review maintenance records of all HVAC equipment serving the BSL3. Visual

inspection of fans, motors, belts, filters, etc.X X

3 Review certification reports of BSCs and HEPA filters X X

4 Verify operation of the autoclave, review BI log, run cycle with datalogger X X

5Complete a BSL3 facility compliance checklist to evaluate all important elements and

performance criteriaX X

6 Verify room differential pressures X X

7 Verify directional airflow X X

8Conduct room envelope integrity testing for all rooms within the BSL3 secondary

containment boundary.X X

9 Verify sensor accuracies using calibrated devices (temp, RH) X X

10Plumbing systems - Operation of any drench showers, eyewash stations and hands

free sinks (hot, cold, drain)X X

11 Waste treatment systems and related SOPs X X

12Electrical systems - Confirm that there are no light bulbs out, no issues with any

outlets (spot check)X X

13 Verify door operation; access controls, closers, latches, interlocks, and alarms X X

14 Bubble-tight damper operation, as applicable X X

15Verify the performance of all zones for stable operation using BAS trend data

X X

16 Local and remote (BAS) alarms, including verification of notifications X X

17

Failure Testing:

1) AHU Supply Fan Failure

2) Exhaust Fan Failure

3) Electrical Power Failure and Restoration of Normal Power

X Backup X X

2 - The exhaust fans serving the BSL-3 can be shut off

3 - U Ky - Building Automation System (require access to view system points, graphics and alarms)

2 - U Ky - Facilities - Mechanical, Electrical, Plumbing and Carpentry (as applicable)

1 - U Ky - EH&S / Lab Manager / Research staff

Cornerstone will need support from multiple parties during this process:

3 - The electrical power to the building can be shut off

12:00pm - 5:00pm on Monday

7:30am - 5:00pm on Tuesday Wednesday

7:30am - 12:00pm onThursday (if needed)

These hours are flexible and can be modified to meet the schedule that will work for onsite personnel

Cornerstone will plan to be onsite between:

All activities are from the Cornerstone Commissioning proposal for Biocontainment Facilities Performance Verification Services dated

March 1, 2021, Scope of Work section.

Performance Verification Activity

Required personnel

Failure testing requirements, please consult with facilities and building occupants to ensure that:

1 - The AHU supply fan(s) serving the BSL-3 can be shut off




Lexington, KY

Name Organization Role Phone Email

Cindy Meier U Ky Research Facility

Manager

859-257-9720 [email protected]

Ilhem Messaoudi U Ky Researcher / Principle

Investigator (PI)

[email protected]

[email protected]

Brandy Nelson U Ky Biosafety Officer 859-550-0333 [email protected]

Kevin Locke U Ky Facilities [email protected]

Dan Cook Cornerstone

Commissioning,

Inc.

PV Provider 603-944-1633 [email protected]

PV Team - Test Participants / Witnesses:

The members of the PV Team include representatives from multiple organizations.

Personnel listed below will be participating in different events and capacities to complete the PV.










Lexington, KY

Documents Available for review? Date / Version / Last Update / Comment

Basis of Design (BOD) / Design Intent Not available

Construction Documents - Drawings and Specification (As built) - Architectural,

Mechanical, Plumbing, Electrical, Security, Life Safety/Fire Protection, etc.

Renovation in 2000, some docs are available;

Floorplans for equipment, electrical, ductwork, and the full specifications.

One line, P&ID for HVAC air system Yes

One line, electrical system Not available

Submittals - Controls / Building Automation System (BAS) - including the

Sequence of Operations (As Built)

Yes

Submittals - Equipment Yes

O&M manuals - For all equip Hard copies available on site for review CM

Testing and Balancing (TAB) report Hard copies available on site for review CM

Commissioning (Cx) report Hard copies available on site for review CM

Performance Verification (PV) report Uky PV report in 2015

Review Entity documentation (Maintenance, Calibration, Certification) Available for review? Date / Version / Last Update / Comment

Maintenance records - All HVAC equipment Not available

Calibration records - Critical control devices Not available

Certifications - Biosafety Cabinets (BSC) Not since 2015, looking to replace BSC per new PI (Ilhem)

Certifications - HEPA filters and housing Not since 2015

System or equipment failures / incidents; utilities Not available

System or equipment failures / incidents; facilities Hard copies available on site for review CM

System or equipment failures / incidents; research equipment Autoclave is inoperable. BN

1 - The supply fan(s) in AHU serving the BSL-3 can be shut off Coordinated with David Moody and Sandy Redmon - FM Med Center

2 - The exhaust fan(s) serving the BSL-3 can be shut off Coordinated with David Moody and Sandy Redmon - FM Med Center

3 - The normal electrical power utility to the building can be shut off Coordinated with David Moody and Sandy Redmon - FM Med Center

Critical Equipment Summary List all vendors/ manufacturers;

Building Automation System (BAS) Tridium

Air Handling Unit (AHU) AHU-2 Buffalo Forge

Exhaust Fans (EF) EF-1, EF-2 Barry Blower

HEPA filter housing AAF/Flanders

Air Terminal Unit manufacturer Phoenix Controls

Room Pressure Monitors (RPM) Air Monitor Corp, Sentry

Electrical systems (Utility feed, generator, ATS, UPS) Not available

Other critical systems/ equipment/components Not available

Question Response

List all modifications and/or changes since initial construction, Cx, PV, etc. Files available on site. CM

Reportable incidents; research staff, laboratory manager, EH&S Lab has been inactive since 2016. BN

Any lessons learned from interaction with research staff, laboratory manager,

EH&S?

N/A BN

Work orders, equipment failure reports Not available

Contractors Precision Air Technologies - BSCs and HEPAs,

Agape Instruments - Autoclave BN

Facilities Management - Questionnaire

Failure testing requirements, confirm with all facilities staff and building occupants to ensure that:

Review background material:




Lexington, KY

Question Response

What type of BSL-3/ABSL-3 level work is conducted in this laboratory? No BSL-3/ABSL-3 work has been conducted in the facility since 2016.

What type of agents are used? N/A

Risk assessment complete? N/A

Has the laboratory been actively used for BSL-3/ABSL-3 level work? No BSL-3/ABSL-3 work has been conducted in the facility since 2016.

Any incidents or events that warrant a decontamination of the laboratory to perform

the PV?

N/A

If yes, was the laboratory successfully decontaminated? N/A

If yes, will the laboratory decontamination be complete before PV work is

scheduled?

N/A

Is the decontamination report available for review? N/A

Are Cornerstone personnel medical evaluations or testing required for entry into the

BSL-3/ABSL-3 laboratory?

Pre and post lab visit?

No

List all required personnel protective equipment (PPE) to meet the risk assessment

(RA):

N/A

PI/ BSO has informed CCx of required safety procedures for work in the BSL-3/ABSL-3

laboratory?

No required safety procedures as lab has been inactive for 5 years.

PI/ BSO has informed and provided CCx with required PPE for work in the BSL-3/ABSL-

3 laboratory?

No required PPE as lab has been inactive for 5 years.

Entity documentation -

Standard Operating Procedure (SOP), Manuals, Plans, etc.Available for review? Date / Version / Last Update / Comment

Biological Safety Manual Yes, 2016-0308

Biomedical Waste Operating Plan Included in biosafety manual.

Biosafety Plan Included in biosafety manual.

BSL-3 Medical Surveillance Plan Yes, 2016-0308

Incident Response Plan Yes, 2016-0308

BSL-3 Training Procedures Included in biosafety manual. Can provide presentations/modules as needed.

BSL-3 Entry and Exit Procedures Included in biosafety manual. Can provide presentations/modules as needed.

BSL-3 Working Safely in a Biosafety Cabinet Included in biosafety manual. Can provide presentations/modules as needed.

BSL-3 Transfer Between Rooms Included in biosafety manual. Can provide presentations/modules as needed.

Laboratory Safety Manual N/A

Facility or Systems manual N/A

Document review Available for review? Date / Version / Last Update / Comment

Do the SOPs meet the needs of the program and laboratory activities? They did in 2016. There is no current high containment work at UK.

Do the SOPs include information on how to manage hazardous (infectious) waste? Yes

Do the SOPs include laboratory accident response protocols? Yes

Have the decontamination methods been evaluated for efficacy? Yes

Is there an integrated pest management program in place? Yes

Are the SOPs tracked with updates based on feedback, continual improvement

program, lessons learned, etc.?

Yes

Do the SOPs describe the interaction of facilities, EH&S, lab manager, researchers,

etc. during maintenance, accidents, and failure events?

Yes

Biosafety Officer (BSO) / Responsible Official (RO) - Questionnaire




Lexington, KY

https://www.cdc.gov/labs/BMBL.html

Compliant? Comments/Notes Recommendation / Solution Updates / Actions Taken

1 The laboratory is separated from areas that are open to unrestricted

traffic flow within the building. Yes

a Laboratory access is restricted. Laboratory doors are lockable in

accordance with institutional policies. Access to the laboratory is

through two consecutive self-closing doors. A clothing change room

and/or an anteroom may be included in the passageway between

the two self-closing doors.

Yes

2 Laboratories have a sink for handwashing. The sink is hands-free

or automatically operated and should be located near the exit door.

If a laboratory suite is segregated into different zones, a sink is also

available for handwashing in each zone.

Yes

3 An eyewash station is readily available in the laboratory. Yes

4 The laboratory is designed, constructed, and maintained to facilitate

cleaning, decontamination, and housekeeping. Partial

Wood doors throughout the lab.

Porous wood paneling on the walls in labs F and

L.

Replace doors (CAP#25)

a Carpets and rugs are not permitted. Yes No carpets or rugs.

b Spaces between benches, cabinets, and equipment are accessible

for cleaning.Yes

c Seams, floors, walls, and ceiling surfaces are sealed. Spaces

around doors and ventilation openings are capable of being sealed

to facilitate space decontamination.

Yes

Access panels are sealed with metal tape.

d Floors are slip-resistant, impervious to liquids, and resistant to

chemicals. Flooring is seamless, sealed, or poured with integral

cove bases.

Yes

e Walls and ceilings are constructed to produce a sealed smooth

finish that can be easily cleaned and decontaminated.Yes

See room integrity test report.

5 Laboratory furniture can support anticipated loads and uses. Yes

a Benchtops are impervious to water and resistant to heat, organic

solvents, acids, alkalis, and other chemicals.Yes

b Chairs used in laboratory work are covered with a non-porous

material that can be easily cleaned and decontaminated with an

appropriate disinfectant.

Yes

Chairs have duct tape or metal foil tape on them

6 All windows in the laboratory are sealed. Yes

7 Illumination is adequate for all activities and avoids reflections and glare that could impede

vision.Yes

8 Vacuum lines in use are protected with liquid disinfectant traps and

in-line HEPA filters or their equivalent. See Appendix A, Figure 11. Filters

are replaced, as needed, or are on a replacement schedule determined

by a risk assessment. Vacuum lines not protected as described are

capped. The placement of an additional HEPA filter immediately prior to

a central vacuum pump is considered.

Partial

Millipore disc HEPA filter on the turrets. Unsure

if this is tied into building system or if it is

independent of non-BSL-3 areas.

Recommend point of use vacuum units, which

can be located inside the BSC.

(CAP #36)

https://www.welchvacuum.com/en-us/piston-

pumps/piston-pump-wob-l-2534

9 A ducted mechanical air ventilation system is required. This system

provides sustained directional airflow by drawing air into the laboratory

from “clean” areas toward “potentially contaminated” areas. The

laboratory is designed such that under failure conditions the airflow will not be reversed at the

containment barrier.

Yes

a A visual monitoring device that confirms directional airflow is

provided at the laboratory entry. Audible alarms to notify personnel

of airflow disruption are considered. Partial

Room Pressure Monitor (RPM) display has all

room pressure values in the corridor, at the

entrance to the lab suite.

Sentry air monitor above each door, display

value is in FPM, not in wc.

Replace with RPM (or other airflow direction

device).

(CAP #8, 9, 10, 11)

b The laboratory exhaust air is not re-circulated to any other area in

the building.Yes

c The laboratory exhaust air is dispersed away from occupied areas

and from building air intake locations or the exhaust air is HEPA

filtered.

Yes

The following items are from the CDC/NIH Biosafety in Microbiological and Biomedical Laboratories (BMBL) 6th Edition guidelines and how the facility meets or

exceeds them.

Biosafety in Microbiological and Biomedical Laboratories (BMBL) - 6th Edition - June 2020

Section IV—Laboratory Biosafety Level Criteria

Biosafety Level 3

D. Laboratory Facilities (Secondary Barriers) (page 48)





Lexington, KY



The following items are from the CDC/NIH Biosafety in Microbiological and Biomedical Laboratories (BMBL) 6th Edition guidelines and how the facility meets or

exceeds them.



Biosafety Level 3


10 BSCs and other primary containment barrier systems are installed and

operated in a manner to ensure their effectiveness. See Appendix A.Yes

a BSCs are installed so that fluctuations of the room air supply and

exhaust do not interfere with proper operations. BSCs are located

away from doors, heavily traveled laboratory areas, and other

possible airflow disruptions.

Yes

b BSCs can be connected to the laboratory exhaust system by either

a canopy connection (Class IIA only) or directly exhausted to the

outside through a hard connection (Class IIB, IIC, or III). Class IIA or

IIC BSC exhaust can be safely recirculated back into the laboratory

environment if no volatile toxic chemicals are used in the cabinet.

Partial

One (1) B2, one (1) fume hood should be

removed.

Consult with research team on needs for BSC

size and type.

(CAP #1, 2)

c BSCs are certified at least annually to ensure correct performance,

or as specified in Appendix A, Part 7.No

d Class III BSCs are provided supply air in such a manner that

prevents positive pressurization of the cabinet or the room. N/A

11 Equipment that may produce infectious aerosols is used within primary

barrier devices that exhaust air through HEPA filtration or other equivalent technology before

being discharged into the laboratory. These HEPA filters are tested annually and replaced as

needed.

N/A

What equipment that may produce aerosols will

be needed by research team?

12 Facility is constructed to allow decontamination of the entire laboratory

when there has been gross contamination of the space, significant

changes in usage, major renovations, or maintenance shutdowns.

Selection of the appropriate materials and methods used to decontaminate the laboratory is

based on the risk assessment.

Partial

Items identified in the room integrity test report

should be corrected.

(CAP#15)

a Facility design consideration is given to means of decontaminating

large pieces of equipment before removal from the laboratory. No

How would a freezer, BSC, or other large piece

of equipment be decontaminated and removed?

13 Enhanced environmental and personal protection may be necessary

based on risk assessment and applicable local, state, or federal

regulations. These laboratory enhancements may include one or more of

the following: an anteroom for clean storage of equipment and supplies

with dress-in, shower-out capabilities; gas-tight dampers to facilitate

laboratory isolation; final HEPA filtration of the laboratory exhaust air;

laboratory effluent decontamination; containment of other piped services; or advanced access

control devices, such as biometrics.

Partial

Ante room, change room, shower, final HEPA on

the exhaust air system,

Floor drains in most spaces, where does it go?

How is it treated?

(CAP#21)

14 When present, HEPA filter housings have gas-tight isolation dampers, decontamination ports,

and/or bag-in/bag-out (with appropriate decontamination procedures) capability. All HEPA

filters are located as near as practicable to the laboratory to minimize the length of potentially

contaminated ductwork. The HEPA filter housings allow for leak testing of each filter and

assembly. The filters and housings are certified at least annually.

No

No gas tight dampers, no decon port, no test

ports, HEPA filters are located on the roof, near

exhaust fans, long run of exhaust duct from the

4th floor to the roof, 4 story building.

(CAP#5)

15 The BSL-3 facility design, operational parameters, and procedures are

verified and documented prior to operation. Facilities are tested annually or after significant

modification to ensure operational parameters are met. Verification criteria are modified as

necessary by operational experience.No

Lab has been decommissioned, not used since

2016.

After all modifications are made will need to re-

commission/verify the performance of all

systems and equipment.

16 Appropriate communication systems are provided between the

laboratory and the outside (e.g., voice, fax, and computer). Provisions

for emergency communication and emergency access or egress are

developed and implemented.

No

No phones inside the laboratory spaces Add phones inside labs.


A.5,

pg 44 A sign incorporating the universal biohazard symbol is posted at the entrance to the laboratory

when infectious materials are present. Posted information includes: the laboratory’s Biosafety

Level, the supervisor’s or other responsible personnel’s name and telephone number, PPE

requirements, general occupational health requirements (e.g., immunizations, respiratory

protection), and required procedures for entering and exiting the laboratory. Agent information

is posted in accordance with the institutional policy.

No

All of the emergency contacts signs are old and

will need to be updated, throughout the lab

space.

Will need to be updated with information

pertaining to new research team.

B.9,

pg 47A method for decontaminating all laboratory wastes should be available in the facility,

preferably within the laboratory (e.g., autoclave, chemical disinfection, incineration, or

other validated decontamination method).

No

Autoclave is not operational Replace autoclave.

(CAP#6,7)


CCx-1An eyewash station must be readily available in the laboratory and meets ANSI Z358.1-

2009.Water is between 60-100 deg F for the 15 minute wash cycle.Yes

CCx-2 Is there a dedicated air handler for BSL-3 supply air? No AHU-2 serves the north side of the HSRB facility.

CCx-3Is there a means of isolating the BSL-3 supply air? This can be done at the common

trunk or at the zone level.Yes

CCx-4

Internal facility appurtenances, such as light fixtures, air ducts, and utility pipes, are

arranged to minimize horizontal surface areas to facilitate cleaning and minimize the

accumulation of debris or fomites.

Yes

The following items are based on Cornerstone Commissioning Biosafety Level Three (BSL-3) laboratory commissioning experience.

These are enhancements and/or best practice based on other BSL-3 laboratory commissioning / PV testing.

Item

Other Sections (A-C)





Lexington, KY

Biosafety in Microbiological and Biomedical Laboratories (BMBL) - 5th

edition Dec 2009

https://www.cdc.gov/labs/pdf/CDC-

BiosafetyMicrobiologicalBiomedicalLaboratories-2009-P.PDF

Biosafety in Microbiological and Biomedical Laboratories (BMBL) - 6th

edition June 2020


Institute for Laboratory Animal Research. Guide for the Care and Use of

Laboratory Animals (the Guide, NRC 2011)

https://www.aaalac.org/the-guide/

https://grants.nih.gov/grants/olaw/guide-for-the-care-and-use-of-laboratory-

animals.pdf

Centers for Disease Control and Prevention (CDC) -

Division of Select Agent and Toxins (DSAT) -

Federal Select Agent Program External (FSAP)

https://www.cdc.gov/cpr/dsat.htm

https://www.selectagents.gov/index.htm

https://www.selectagents.gov/sat/list.htm

https://www.selectagents.gov/compliance/guidance/index.htm

CDC DSAT - Select Agent Grams - 8/05/11 - Policy on BMBL 5th edition

Laboratory Facilities (Secondary Barriers) Standards

(Updated on 11/20/14 )

https://www.selectagents.gov/sagrams.html

https://www.selectagents.gov/sagrams-2008-2015.html

ANSI Z9.14 - 2020 - Testing And Performance-Verification Methodologies

For Ventilation Systems For Biosafety Level 3 (BSL-3) And Animal

Biosafety Level 3 (ABSL-3) Facilities

https://store.assp.org/PersonifyEbusiness/Store/Product-

Details/productId/207427871

National Institutes of Health (NIH) - Design Requirements Manual (DRM) https://www.orf.od.nih.gov/TechnicalResources/BiomedicalandAnimalResea

rchFacilitiesDesignPoliciesandGuidelines/Pages/default.aspx

NIH Recombinant/Synthetic Nucleic Acid (r/sNA) Guidelines https://osp.od.nih.gov/wp-content/uploads/NIH_Guidelines.pdf

NIH BSL-3 Certification Standards https://www.orf.od.nih.gov/TechnicalResources/Bioenvironmental/Docume

nts/BSL3CertificationGuidelinesFINAL_508.pdf

United States Department of Agriculture (USDA) -

Agricultural Research Service (ARS) -

ARS Design Standards (242.1M-ARS)

https://www.ars.usda.gov/

https://govtribe.com/file/government-file/attachment-9-ars-design-

standards-242-dot-1m-ars-dot-pdf-1

World Health Organization (WHO) -

Laboratory Biosafety Manual - Third Edition

https://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_

LYO_2004_11/en/

Canadian Biosafety Standards and Guidelines https://www.canada.ca/en/public-health/services/canadian-biosafety-

standards-guidelines.html

Department of the Army Pamphlet - 385-69

Safety Standards for Microbiological and Biomedical Laboratories

https://armypubs.army.mil/epubs/DR_pubs/DR_a/pdf/web/p385_69.pdf

Interim Laboratory Biosafety Guidelines for Handling and Processing

Specimens Associated with Coronavirus Disease 2019 (COVID-19)

https://www.cdc.gov/coronavirus/2019-nCoV/lab/lab-biosafety-

guidelines.html

NSF/ANSI Standard 49 – 2014, Biosafety Cabinetry: Design, Construction,

Performance, and Field Certification.

https://standards.nsf.org/apps/group_public/download.php/36078/NSF_49-

2014.pdf

BSC A2 with thimble alarm requirement;

Memo dated Oct 15, 2015: to be fitted with an Airflow Monitor that

provides a visual and audible alarm.

https://d2evkimvhatqav.cloudfront.net/documents/NSF_Biosafety_Cabinet_

Memo_Canopy_Connections.pdf

Institute of Environment Sciences and Technology (IEST)

IEST-RP-CC001: HEPA AND ULPA FILTERS - BASIC PROVISIONS

https://www.iest.org/Standards-RPs/Recommended-Practices/IEST-RP-

CC001IEST-RP-CC021: TESTING HEPA AND ULPA FILTER MEDIA https://www.iest.org/Standards-RPs/Recommended-Practices/IEST-RP-

CC021

IEST-RP-CC034: HEPA AND ULPA FILTER LEAK TESTS https://www.iest.org/Standards-RPs/Recommended-Practices/IEST-RP-

CC034

References (As applicable)

HEPA (high efficiency particulate air) references:

Biosafety Cabinet (BSC) references:


https://www.cdc.gov/labs/pdf/CDC-BiosafetyMicrobiologicalBiomedicalLaboratories-2009-P.PDF

https://www.cdc.gov/labs/pdf/CDC-BiosafetyMicrobiologicalBiomedicalLaboratories-2009-P.PDF












https://store.assp.org/PersonifyEbusiness/Store/Product-Details/productId/207427871

https://store.assp.org/PersonifyEbusiness/Store/Product-Details/productId/207427871

https://www.orf.od.nih.gov/TechnicalResources/BiomedicalandAnimalResearchFacilitiesDesignPoliciesandGuidelines/Pages/default.aspx

https://www.orf.od.nih.gov/TechnicalResources/BiomedicalandAnimalResearchFacilitiesDesignPoliciesandGuidelines/Pages/default.aspx

https://osp.od.nih.gov/wp-content/uploads/NIH_Guidelines.pdf

https://www.orf.od.nih.gov/TechnicalResources/Bioenvironmental/Documents/BSL3CertificationGuidelinesFINAL_508.pdf

https://www.orf.od.nih.gov/TechnicalResources/Bioenvironmental/Documents/BSL3CertificationGuidelinesFINAL_508.pdf





https://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_2004_11/en/

https://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_2004_11/en/

https://armypubs.army.mil/epubs/DR_pubs/DR_a/pdf/web/p385_69.pdf

https://www.cdc.gov/coronavirus/2019-nCoV/lab/lab-biosafety-guidelines.html

https://www.cdc.gov/coronavirus/2019-nCoV/lab/lab-biosafety-guidelines.html

https://standards.nsf.org/apps/group_public/download.php/36078/NSF_49-2014.pdf

https://standards.nsf.org/apps/group_public/download.php/36078/NSF_49-2014.pdf

https://d2evkimvhatqav.cloudfront.net/documents/NSF_Biosafety_Cabinet_Memo_Canopy_Connections.pdf

https://d2evkimvhatqav.cloudfront.net/documents/NSF_Biosafety_Cabinet_Memo_Canopy_Connections.pdf

https://www.iest.org/Standards-RPs/Recommended-Practices/IEST-RP-CC001








Lexington, KY

A/E Architect/ engineer FH Fume Hood

AAALACAssociation for Assessment and Accreditation of Laboratory

Animal Care (vivarium)FPM Feet Per Minute (air velocity)

ABD As built drawings FPTFunctional Performance Test,

Facility Performance Test (APV)

ABSA American Biological Safety Association FSAP Federal Select Agent Program

ACH Air Changes per Hour HEPA High Efficiency Particulate Air

AHR Animal holding room HVAC Heating, Ventilation, and Air Conditioning

AHU Air Handling Unit HX Heat Exchanger

ANSI American National Standards Institute Hz Hertz (frequency)

APR Air Pressure Resistant door IEST Institute of Environment Sciences and Technology

APV Annual Performance Verification (BSL-3) IST Integrated System Tests (Failure Testing)

ASHRAEAmerican Society of Heating, Refrigerating and Air-

Conditioning EngineersIVC Individually Ventilated Cage

ASSP American Society of Safety Professionals LEED Leadership in Energy and Environmental Design

ATS Automatic Transfer Switch MRI Magnetic Resonance Imaging

ATU Air Terminal Unit N/A Not applicable

BAS/BMS Building automation system/ building management system N95Particulate filtering facepiece respirators, that filters at least

95% of airborne particles.

BD Balancing Damper NHP Non Human Primate

BFP/RPZ Back Flow Preventer NIH National Institutes of Health

BI Biological Indicator (decontamination/autoclave) NSF National Sanitation Foundation (BSC NSF 49)

BMBL Biosafety in Microbiological and Biomedical Laboratories O&M Operations and Maintenance

BOD Basis of design OA Outside Air

BSC Biosafety Cabinet OAD Outside Air Damper

BSD/ BTD Bioseal damper / bubble tight damper OAT Outside Air Temperature

BSL-3 Biosafety Level three (ABSL-3 = Animal BSL-3) OPR Owners project requirements

BSO Biosafety Officer P2P Point to Point

CA Compressed Air, Combustion Air (boiler) PAPR Powered Air Purifying Respirator

CAV Constant air volume PCR Polymerase Chain Reaction

CB Change bulletin, construction bulletin PE Professional engineer, project executive

CD Chlorine Dioxide (gaseous decontamination agent) PHA Potential Hazard Analysis

CD

DD

SD

Construction Documents

Design Development

Schematic Design

PI Principal Investigator (research)

CDC Center for Disease Control PM Project manager, preventative maintenance

CFM Cubic Feet per Minute (airflow volume) PPE Personal Protective Equipment

CM/GC Construction manager / general contractor PSI Pound per Square Inch (pressure)

CO Change Order PVPerformance Verification (BSL-3),

Photovoltaic

CO Carbon Monoxide PVC Performance Verification Criteria

CO2 Carbon Dioxide RA Risk Assessment

CR Change Request RFI Request For Information

CSG Clean Steam Generator RH Relative Humidity

CV Constant Volume, Coefficient Value, Check Valve, Control Valve RIT Room Integrity Testing

CX Commissioning RO Reverse Osmosis (water), Responsible Official (BSL-3)

CxA Commissioning Authority RODI Reverse Osmosis Deionized (water)

DID Design Intent Document RPM Room Pressure Monitor, Revolutions Per Minute

DLT Duct Leakage Testing SA Supply Air

DP Differential Pressure SAD Supply Air Damper

DRM Design Requirements Manual SASP Supply Air Static Pressure

DSAT Division of Select Agents and Toxins (CDC) SAT Supply Air Temperature

EA Exhaust Air SAV Supply Air Valve

EAD Exhaust Air Damper SOO Sequence of Operation

EASP Exhaust Air Static Pressure SOP Standard operating procedure

EAT Exhaust Air Temperature SP Setpoint

EAV Exhaust Air Valve TAB Testing, Adjusting and Balancing

EC, FP, PC Elec contractor, fire protection, plumbing contractor UPS Uninterruptible Power Supply

EDS Effluent Decontamination System USDA United States Dept of Agriculture

EF Exhaust Fan USGBC U.S. Green Building Council

Acronyms




Lexington, KY

Acronyms

EF Exhaust Fan VAV Variable Air Volume

EH&S Environmental Health and Safety VCR Ventilated Cage Rack

EOR Engineer of Record VD Volume Damper

ERP Energy Recover Pump VFD Variable Frequency Drive

VHP Vaporized Hydrogen Peroxide




Lexington, KY


Compliant? Comments/Notes

1 ABSL-3 facilities should be separated from the general traffic patterns of

the building and restricted as appropriate. Consider placing animal areas away from exterior

walls of buildings to minimize the impact from the outside environment temperatures.

a External facility doors are self-closing and self-locking.

b Access to the animal facility is restricted.

c Doors to areas where infectious materials and/or animals are housed open inward, are self-

closing, are kept closed when experimental animals are present, and are never propped open.

d Entry into the containment area is via a double-door entry, which

constitutes an anteroom/airlock and a change room. Exit showers

may be considered based on risk assessment. An additional

double-door anteroom or double-doored autoclave may be provided

for movement of supplies and wastes into and out of the facility.

2 A handwashing sink is located at the exit of the areas where infectious

materials and/or animals are housed or manipulated. Additional sinks for handwashing are

located in other appropriate locations within the facility. If the animal facility has segregated

areas where infectious materials and/or animals are housed or manipulated, a handwashing sink

is also available near the exit from each segregated area.

a The sink is hands-free or automatically operated.

b Emergency eyewash and shower are readily available, easily

accessible, and appropriately maintained.

c Sink traps are filled with water and/or appropriate disinfectant or

sealed to prevent the migration of vermin and gases.

d Floor drains are maintained and filled with water and/or appropriate

disinfectant or sealed to prevent the migration of vermin and gases.

3 The animal facility is designed, constructed, and maintained to facilitate

cleaning, decontamination, and housekeeping. The interior surfaces

(e.g., walls, floors, and ceilings) are water-resistant.

a Floors are slip-resistant, impervious to liquids, and resistant to

chemicals. Flooring is seamless, sealed, or poured with integral

cove bases. Floors slope to drain, if present.

b Penetrations in floors, walls, and ceiling surfaces are sealed,

including openings around ducts, outlets, switch plates, and

doorframes, to facilitate pest control, proper cleaning, and decontamination. Walls, floors, and

ceilings form a sanitizable and sealed surface.

c Internal facility fixtures, such as light features, air ducts, and utility

pipes, are designed and installed to minimize horizontal surface

areas to facilitate cleaning and minimize the accumulation of debris

or fomites.

d External windows are not recommended; if present, they are sealed

and resistant to breakage.

e Illumination is adequate for all activities and avoids reflections and

glare that could impede vision.

4 Furniture is minimized and can support anticipated loads and uses.

a Benchtops are impervious to water and resistant to heat, organic

solvents, acids, alkalis, and other chemicals.

b Chairs used in animal areas are covered with a non-porous material

that can be easily cleaned and decontaminated with an appropriate

disinfectant and sealed to prevent harboring of insects/vermin.

c Equipment and furnishings are carefully evaluated to minimize

exposure of personnel to pinch points and sharp edges and corners.

5 Ventilation is provided in accordance with the Guide for the Care and

Use of Laboratory Animals. (3)

a Ventilation system design considers the heat and high moisture

load produced during the cleaning of animal rooms and the cage

wash process.

b The direction of airflow into the animal facility is inward; animal

rooms maintain inward directional airflow compared to adjoining

hallways. A visual monitoring device, which confirms directional

airflow, is provided at the animal room entrance.

The following items are from the CDC/NIH Biosafety in Microbiological and Biomedical Laboratories (BMBL) 6th Edition guidelines and how the facility meets or exceeds them.


Section V—Vertebrate Animal Biosafety Level Criteria for Vivarium Research Facilities

Animal Biosafety Level 3

D. Animal Facilities (Secondary Barriers) (page 94)





Lexington, KY








c A ducted exhaust air ventilation system is provided. Exhaust air is

discharged to the outside without being recirculated to other rooms.

This system creates directional airflow, which draws air into the

animal room from “clean” areas and toward “contaminated” areas.

d The exhaust air is dispersed away from occupied areas and from

building air intake locations or the exhaust air is HEPA-filtered.

e The ABSL-3 animal facility is designed such that under failure

conditions the airflow will not be reversed at the containment barrier.

Alarms are considered to notify personnel of ventilation and HVAC

system failure.

6 Cages are decontaminated prior to removal from the containment barrier

and prior to washing in a mechanical cage washer. The cage wash area

is designed to accommodate the use of high-pressure spray systems,

humidity, strong chemical disinfectants, and 180°F water temperatures

during the cage/equipment cleaning process.

7 BSCs and other primary containment barrier systems are installed and

operated in a manner to ensure their effectiveness. See Appendix A.

a BSCs are installed so that fluctuations of the room air supply and

exhaust do not interfere with proper operations. BSCs are located

away from doors, windows that can be opened, heavily traveled

areas, and other possible airflow disruptions.

b BSCs can be connected to the animal facility exhaust system by

either a canopy connection (Class IIA only) or directly exhausted to

the outside through a hard connection (Class IIB, IIC, or III). Class

IIA or IIC BSC exhaust can be safely recirculated back into the

animal facility environment if no volatile toxic chemicals are used in

the cabinet.

c BSCs are certified at least annually to ensure correct performance,

or as specified in Appendix A, Part 7.

d Class III BSCs are provided supply air in such a manner that

prevents positive pressurization of the cabinet or the animal room.

8 Equipment that may produce infectious aerosols is contained in primary

barrier devices that exhaust air through HEPA filtration, or other equivalent technology, before

being discharged into the animal facility. These HEPA filters are tested annually and replaced as

needed.

9 All vacuum lines are protected with HEPA filters, or their equivalent, or

are capped. Vacuum lines in use are protected with liquid disinfectant

traps and in-line HEPA filters or their equivalent. See Appendix A, Figure

11. Filters are replaced, as needed, or are on a replacement schedule

determined by a risk assessment. The placement of an additional HEPA

filter immediately prior to a central vacuum pump is considered.

10 An autoclave is available within the containment barrier. The autoclave is utilized to

decontaminate infectious materials and waste before moving these materials to the other areas

of the facility. If not within the containment barrier, special practices are developed for the

transport of infectious materials to designated alternate locations for decontamination. A

validated alternative process (e.g., alkaline digestion, incineration) may be used for

decontamination and disposal of carcasses.

11 The ABSL-3 facility design, operational parameters, and procedures are

verified and documented prior to operation. Facilities are tested annually

or after significant modification to ensure operational parameters are

met. Verification criteria are modified as necessary by operational

experience.

12 Enhanced environmental and personal protection may be necessary

based on risk assessment and applicable local, state, or federal regulations. These enhancements

may include one or more of the following: an anteroom for clean storage of equipment and

supplies with dress-in, shower-out capabilities; gas-tight dampers to facilitate animal room

isolation; final HEPA filtration of the animal room exhaust air; animal room effluent

decontamination; containment of other piped services; or advanced access control devices, such

as biometrics.





Lexington, KY









A.8,

pg 89 A sign is posted at the entrance to the animal room when infectious agents are present. Posted

information includes: the universal biohazard symbol, the room’s Animal Biosafety Level, the

supervisor’s or other responsible personnel’s name and telephone number, PPE requirements,

general occupational health requirements (e.g., immunization, respiratory protection), and

required procedures for entering and exiting the animal room. Agent information is posted in

accordance with the institutional policy.


CCx-1An eyewash station must be readily available in the laboratory and meets ANSI Z358.1-

2009.Water is between 60-100 deg F for the 15 minute wash cycle.CCx-2 Is there a dedicated air handler for ABSL-3 supply air?

CCx-3Is there a means of isolating the ABSL-3 supply air? This can be done at the common trunk

or at the zone level.

CCx-4

A method for decontaminating all laboratory wastes should be available in the facility,

preferably within the laboratory (e.g., autoclave, chemical disinfection, incineration, or

other validated decontamination method).

CCx-5

Any HEPA filter housing for the BSL-3 needs to have an injection, upstream, and

downstream (scanning) port to allow for scan testing.

(Ensure appropriate duct length is available to meet upstream and downstream

requirements for the test.)

The following items are based on Cornerstone Commissioning Animal Biosafety Level Three (ABSL-3) laboratory commissioning experience.

Item

Other Sections (A-C)




PV Report - 2021


Corrective Action Plan

Corrective Action PlanPrint date: 2021-08-10

University of Kentucky, HSRB 4th floor BSL-3

Lexington, KY

Project #: 1929

Item # System or Device Problem, Issue, Root Cause Effect/Impact Responsible Party Solution Updates / Actions Taken Status

1 Fume hood in 464FIf this is not required for the planned research, recommend

removing from the lab.

Facility

PerformanceUniv of Kentucky

Remove fume hood.OPEN

2Biosafety Cabinets

(BSC)

All six (6) of the BSCs are out of certification.

These are older models, the manufacture may not support

these models anymore, repair parts and filters may be difficult

to source.

Equipment

OperationUniv of Kentucky

Recommend consulting with research team on requirements

for BSCs in the BSL-3 for size and quantity needed.OPEN

3Exhaust duct

construction

Observed the main exhaust duct trunk is welded stainless steel

from the exhuast fans and HEPA housing on the roof to the

Phoenix valve in the lab. After the Phoenix valve, the ductwork

is spiral/square galvanized, then metal flexible duct

connections to the grilles.

Galvanized is prone to corrosion from decontamination agents.

Flexible duct work is leaky.

Facility


Recommend hard duct connections from the Phoenix valve to

the grilles.

Consider replacing the galvanized and flexible ductwork.

OPEN

4

Bag In Bag Out

Enclosure, HEPA

filters on the main

exhaust

HEPA filters are out of certification.

Industry

GuidelineUniv of Kentucky

Test and certify the HEPA filters according to industry

guidelines.OPEN

5

Bag In Bag Out

Enclosure, HEPA

filters on the main

exhaust

No isolation dampers on inlet and outlet, decontamination

ports, or test ports.

This can make decontaminating, replacing and

testing/certification difficult.

Industry


Consider replacing the HEPA housing.

OPEN

6 AutoclaveThe autoclave is not operational. Industry


Replace the autoclave. OPEN

7 Autoclave

The autoclave is not ideally situated in the layout of the

laboratory. The service side is located inside of containment,

any maintenance personnel will have to gown up to service the

autoclave. Any tools and equipment would have to be

decontaminated when done.

Maintenance Univ of Kentucky

If a layout change is planned, consider making room large

enough to work on the autoclave.

OPEN

8Room Pressure

Monitors (RPM)

The DP readout panel in the corridor, all of the values

displayed are off by a factor of ten (10).

Example:

464G/464H

Display = -0.50" wc

Measured value = -0.05" wc

Facility


Make all corrections that were identified.

OPEN

9Room Pressure

Monitors (RPM)

The following RPM are installed on the wrong side of the door.

RPM should be located on the clean side, so personnel

entering the lab can verify they have proper directional airflow.

464G/464F (inside F)

464G/464L (inside L)

Facility


Discuss if this is a problem or not.

OPEN

10Room Pressure

Monitors (RPM)

The Air Monitor Corp/Sentry RPM display values are in feet per

minute (FPM), typically these are in inches of water column ("

wc). But, if the lab personnel are trained to look for FPM, this

may not be an issue.

Facility



OPEN




Lexington, KY

Project #: 1929


11Room Pressure

Monitors (RPM)

Some of the RPM are piped to monitor the pressure across

multiple doors, this can be an alarming issue if the doors are

propped open or do not close all the way, they will not alarm in

the current configuration unless there is a complete loss of

exhaust to the whole laboratory.

Ante room 464 / Airlock / Lab 464G

Ante room 464 / Locker 464A / Decon 464D

Ante room 464 / Locker 464Q / Decon 464M

Facility



OPEN

12Room differential

pressure setup

Typically BSL-3 room differential pressures (DP) are setup to

have a value between -0.03 to -0.09" wc across each door, the

mid value that most aim for is -0.05" wc.

See FPT - Room Pressures, section 1.4 for more details.

Facility


Make adjustments to room airflows to achieve desired room

pressures.

Suggest hiring test and balance (TAB) contractor to confirm all

airflows.

OPEN

13Room differential

pressure setup

The Air Monitor Corp/Sentry RPM - The visual and/ or audible

alarms are not setup or working properly.

See FPT - Room Pressures, section 1.4 for more details.

Facility



OPEN

14 Door hardwareThe door between Decon 464M and Lab 464L does not close

and latch on its own.

Facility


Perform maintenance on all door hardware.OPEN

15Secondary

Containment Barrier

Correct all items in the Room Integrity Test Report (FPT section

1.7).Facility



OPEN

16Secondary

Containment Barrier

Small dings in walls should be repaired, re-painted; examples:

Airlock, decon D/M, anteroom, AHR H/J, Lab F/L/G.Facility



OPEN

17Secondary

Containment Barrier

Cleanliness of whole lab; floors, walls, ceiling, devices (exhaust

grilles, sinks, eye wash, toilet, etc) should be cleaned before

BSL-3 work is conducted.

Facility


Clean the entire laboratory.

OPEN

18 Temperature control

The temperature control of some of the spaces does not

appear to be able to meet setpoint.

Some of the sensors (discharge air) are beyond the range or do

not appear to be indicating correctly.

The reheat valve (HWV on BAS gfx) does not appear to be

indicating correctly, according to the discharge air temp.

See FPT - Temp & RH sensor, section 1.8 for more details.

Facility


Troubleshoot and make all corrections required.

OPEN

19 Graphics

There are rooms missing or incorrect values listed on the

Building Automation System (BAS) graphics (gfx).

See FPT - Temp & RH sensor, section 1.8 and airflow, section

1.15 for more details.

Facility



OPEN

20 Hands free sinksBatteries need to be replaced and operation confirmed in the

following rooms: 464F, 464J, 464L.

Facility


Replace batteries.OPEN

21 Floor drains

The following spaces have floor drains;

They need to be cleaned of dirt/hair/debris: 464J, 464L

These should be capped: 464G (quantity of two).

Facility


Perform maintenance on all floor drains.

OPEN

22Animal Watering

System

Room 464J has abandoned animal watering system (AWS). Facility


If this is not in use, suggest removing all equipment, cap

utilities and patch all holes. OPEN

23Light fixtures and

bulbs

Room 464L appears to have some light bulbs out. Facility


Before BSL-3 is used, recommend replacing all of the light

bulbs in the facility.OPEN




Lexington, KY

Project #: 1929


24 Light switches

Animal holding rooms - There are two (2) light switches

located in 464G.

464J - Both switched are red (emer generator power?), right

switch controls left two light fixtures, left switch controls right

two light fixtures.

464H - Red (left) and grey (right), left switch controls left two

light fixtures, right switch controls right two light fixtures.

Facility


Why are these different?

Do the window light panes require red film?

OPEN

25 Door material

The doors in the facility are wood, and due to their age most

have dings, cracks, and chunks missing. This material is porous

and hard to decontaminate.

Recommend replacing all doors in the facility.

Cleanability Univ of Kentucky

Replace doors with non porous type.

OPEN

26 Door hardwareThe door between Decon 464D and Locker 464A, the handle

gets stuck down after use.

Facility


Perform maintenance on all door hardware.OPEN

27 Access controlThe occupancy sensor in 464, will unlock the door, is this a

security concern?

Facility


Discuss if this is a problem or not.OPEN

28Isolation dampers,

supply and exhaust

During testing, observed that some of the isolation dampers

do not block air from AHU or exhaust fan.

Tested multiple times, with associated supply and exhaust

open and closed, same results.

Could be not closing all the way, or the gasket material is

compromised, but the isolation dampers are not completely

closing off when commanded closed.

Equipment


Investigate why these dampers are not functioning. Make

repairs as necessary.

OPEN

29Isolation damper

control panel

Panel in 464G, during testing noted the following:

464H, supply damper 7 - switch is backwards in panel, switch

closed = damper open, switch open = damper closed.

464J, supply damper 8 - When switch was closed, green light

stayed on indicating open, mag gauge changed from 0.0" to

0.5".

464F, exhaust damper C - closed/ decon red light burned out,

not illuminated when in closed position.

Equipment



OPEN

30Purge key switch

function

The Purge key switch in Corridor 400N, how does this

function?

I was not able to test this.

Equipment


Will it still be required?

OPEN

31 AHU failure test

During this test, found issues with BAS programming and VFD

settings. Eric Hess has list of items that need to be corrected. Facility



OPEN

32 Exhaust fan (EF-1)

This fan bearings should be checked, while in operation sounds

like the bearings are in need of replacement.

Both fans may be past their service life.Maintenance Univ of Kentucky

If fans are replaced, this is a non issue.

OPEN

33Exhaust fan

performance

Both fans run 100% all the time, on motor starters. When one

fan fails, the rooms go less negative than normal or go neutral.

There is no redundancy in this system.

Facility


Replace fans that will provide redundancy.

OPEN

34 Power failure test

The BAS network controller / JACE (Java Application Control

Engine) is not on UPS, during power failure events the BAS

cannot see what is happening with equipment serving the BSL-

3.

Facility


Add UPS to all critical controllers.

OPEN




Lexington, KY

Project #: 1929


35 Control system

Older BAS equipment, (micro zone, Barber-Coleman). There

are no companies that will support these older controllers and

there are no longer replacement parts.Maintenance Univ of Kentucky

Update control system for exhuast fans and room controls

(airflow control, pressure monitoring, temperature control,

alarming, trending, graphics, etc.)OPEN

36Laboratory vacuum

system

BMBL 6th, D, 8.

Millipore disc HEPA filter on the turrets. Unsure if this is tied

into building system or if it is independent of non-BSL-3 areas. Industry


Recommend point of use vacuum units, which can be located

inside the BSC.

(CAP #36)

https://www.welchvacuum.com/en-us/piston-pumps/piston-

pump-wob-l-2534

OPEN

37 Laboratory equipment

BMBL 6th, D, 11.

What equipment that may produce aerosols will be needed by

research team?

Industry


Identify equipment needed and ensure the equipment has

safety mechanisms to minimize risks. OPEN

38Decontamination of

large quipment

BMBL 6th, D, 11.a.

How would a freezer, BSC, or other large piece of equipment

be decontaminated and removed?

Industry


If a layout change is planned, consider making room large

enough to decon equipment. OPEN

39Communication

equipment

BMBL 6th, D, 16.

No phones inside the laboratory spaces.Industry


Add phones inside labs.

OPEN



PV Report - 2021


Facility Performance Tests and Data

Facility Performance Tests University of Kentucky


Lexington, KY

Project #: 1929

Rev # Issue Date Issued By / Initials Revision Description1 2021-07-19 Cornerstone Cx / AT Initial Draft

Test # Test Date Performed By Test Description1 2021-07-26 Cornerstone Cx / DC

2 2021-07-27 Cornerstone Cx / DC3 2021-07-28 Cornerstone Cx / DC

Instrument Type Calibration DateMicromanometer Dec 2020

Temp and RH meter Dec 2020

IR temp (gun) N/A

Smoke bottle N/A

Electrical Circuit Tester N/A

1.0 Test Purpose

The purpose of this facility performance test is to verify and document that all of the systems that serves the BSL-3 space are in

good working order. This includes; electrical, plumbing, HVAC, building automation, security, access control and primary

containment equipment.

Document Control

Test Control

Test Equipment

Manuf/Model

Fluke 922

BK Precision 720

Fluke IR temp gun

Regin FPKlein/ Ideal


Facility Performance Test University of Kentucky


Lexington, KY

1

2

3

Room# BSC Tag# BSC Type Make Model S/N Certifier Cert Due 1 2 3 Notes

464F Fume hood N/A Hamilton Safe Aire N/A N/AExpired Sep

2016N/A N/A N/A 1

464F 464F Class II Type B2 Labconco 36210 268088Precision Air

Technology

Expired Sep

2016Yes Yes N/A 1

464H A 613226Class II Type A2,

recirculatingLabconco 3621204 DELTA 011218476F

Precision Air

Technology

Expired Oct

2019Yes Yes N/A

464J A 568783Class II Type A2,

recirculatingLabconco 36212-04 AC 706229

Precision Air

Technology

Expired Sep

2016Yes Yes N/A

464J A 665623Class II Type A2,

recirculatingNuAire NU-425-400 111024112806

Precision Air

Technology

Expired Sep

2016Yes Yes N/A

464L N/AClass II Type A2,

recirculatingLabconco 362080413281 061062103 AD

Precision Air

Technology

Expired Oct

2019Yes Yes N/A

464L N/AClass II Type A2,

recirculatingLabconco 362080413281 061062105 AD

Precision Air

Technology

Expired Sep

2016No No N/A 2

1

2

CDC/NIH Biosafety in Microbiological and Biomedical Laboratories (BMBL) Appendix A – Primary Containment for Biohazards: Selection,

Installation and Use of Biological Safety Cabinets

NSF/ANSI Standard 49 – 2014, Biosafety Cabinetry: Design, Construction, Performance, and Field Certification.

NSF/ANSI Standard 49-2014 - Memo dated Oct 15, 2015: to be fitted with an Airflow Monitor that provides a visual and audible alarm.

Reference:

Grey outlet, others plugged into red outlets, not sure if grey is Epower, cant see tag / label behind BSC

Planning to be removed and replaced

1.1 - Biosafety Cabinet (BSC)

Record the information for the Biosafety Cabinet (BSC) which is the primary containment equipment for the BSL-3.

Biosafety cabinet (BSC) - Primary Containment

Notes

Certification sticker in place on BSC? (yes/no)

Plugged into Emergency power panel/ circuit or UPS power outlet? (yes/no)

Alarm (audible and visual) functions? (yes/no)




Lexington, KY

HEPA

Housing tag

#

Make Model S/N ServesPre

filter DP

HEPA

filter DP

Certification

sticker in place?Certifier:

Certification

Due:Notes

FF-2 AAF/Flanders

Bag In/ Bag Out

Enclosure, 2 High

by 2 Wide, Pre and

HEPA filter housing

444-777 BSL-3 / EF-1 N/A 0.3 ExpiredPrecision Air

Technology

Cannot read

sticker info

FF-2 AAF/Flanders

Bag In/ Bag Out

Enclosure, 2 High

by 2 wide, Pre and

HEPA filter housing

444-777 BSL-3 / EF-2 N/A 0.6 ExpiredPrecision Air

Technology

Cannot read

sticker info

IEST-RP-CC-006.3-2004

Notes

1.2 - Biosafety equipment

Record the information for the High Efficiency Particulate Air (HEPA) filter housing

High Efficiency Particulate Air (HEPA) Housing

Reference:

IEST-RP-CC002.4




Lexington, KY

Cycle Name Pressure (psi)Temperature

(°C)Duration (min) Cool down (min)

Review Biological Indicator (BI) log book

Frequency of BI runs Results show positive kill?

Unit temp and

pressure tape

readout is legible?

Door interlock

functions?

Datalogger results

match unit tape

readout and cycle

parameters?

Attach copy/ photo of

BI log book page (most

recent)

Attach copy/ photo of autoclave tape

readout (most recent)

Attach data logger

graph for temp and

pressure with tape

read out for cycle

tested

Unit / Tag # Data analysis Temperature (°C) Pressure (psi)

Max value

Count > or = (in minutes )

Count threshold value

1.3 - Autoclave

Cycle parameters

Notes

Inoperable, needs to be replaced, recommend installing so it can be worked on without going into containment, in the ante room




Lexington, KY

Room Name Room # Room Name Room #RPM goes

to zero?Test the room DP alarm

Corridor 400N → Ante 464 N/A N/A -0.047 Yes N/A N/A

Ante Room 464 → Airlock - -0.446 -559 FPM -0.013 Yes -490 FPM no alarmstays negative, monitor piped across multiple

doorsAirlock - → Lab 464G N/A N/A -0.02 Yes N/A N/A

Lab 464G → Lab 464F 0.588 +649 FPM -0.051 Yes +200 FPMVisual, change from Normal

(green) to Caution (yellow)

RPM is on wrong side of door, F, should be in G.

Sentry RPM is set for a positive room, always in

caution.

Lab 464G → AHR 464H -0.288 -470 FPM -0.035 Yes +258 FPMRed, alarm, visual only,

after 60 sec

Lab 464G → AHR 464J -0.962 -1134 FPM -0.14 Yes 126 FPMRed, alarm, audible and

visual after 60 sectoo neg

Lab 464G → Lab 464L 0.511 588 FPM -0.019 Yes 0 FPMVisual, change from Normal

(green) to Caution (yellow)

RPM is on wrong side of door, L, should be in G.

Sentry RPM is set for a positive room, always in

caution.Decontam 464D → Lab 464F -0.511 -704 FPM -0.055 Yes -130 FPM Caution light only

Decontam 464M → Lab 464L -0.61 -692 FPM -0.055 Yes 0 FPMRed, alarm, audible and

visual after 60 sec

Ante Room 464 → Locker 464A -0.563 -682 FPM -0.01 Yes -579 FPM no alarmstays negative, monitor piped across multiple

doorsLocker 464A → Decontam 464D N/A N/A -0.025 Yes N/A

Ante Room 464 → Locker 464Q -0.349 -412 FPM 0.006 Yes -418 FPM no alarmstays negative, monitor piped across multiple

doors, M not Q on corr displayLocker 464Q → Decontam 464M N/A N/A -0.013 Yes N/ALocker 464A Neutral Toilet 464B N/A N/A 0 Yes N/ALocker 464Q Neutral Toilet 464P N/A N/A 0 Yes N/A

Sentry RPM, has room status (normal=green, cuation=yellow, alarm=red)CAUTION 1-2

The values on the panel in corridor 400N, off by factor of 10, example, shows -0.50", actual is -0.050".

1.4 - Room Pressures

Perform the following:

1. Verify that all doors are closed in the BSL-3

2. Record the values from the Room Pressure Monitor (RPM) and BAS graphics.

3. Using a micromanometer (Fluke 922) measure the room pressure.

4. Verify the polarity of the readings with a smoke bottle.

5. Open the door to the space and verify that the RPM and BAS indicates 0.00".

6. Leave door open and verify that the room DP / door open alarm functions. Note the time it takes once the RPM reads 0.00" for the alarm to activate.

Notes

?

Reference

(Low -)

To

(High +)

?

Custom High limit?Custom Low limit?Alarm trip pointAlarm delay time

RPM setup???

CCx

Verify

polarity

with

smoke?

With door open

Manufacturer:Model:

Corr

display

Room

Pressure

Monitor

Airflow

Direction

Sentry

Room

Pressurizati

on Monitor

Range:

Air Monitor Corporation / CustomSentry

Room Pressure Monitor (RPM) info

BAS interface?

?




Lexington, KY

1.4 - Room PressuresFloorplan for reference:




Lexington, KY

Top Middle Bottom

Corr 400N → Ante Room 464 → → Neutral Yes

Ante Room 464 → Locker 464A → → → Yes

Ante Room 464 → Locker 464Q → → ← Yes

Ante Room 464 → Airlock - → ← → Yes

Airlock - → Lab 464G → → ← Yes

Locker 464A → Decontam 464D → → → Yes

Decontam 464D → Lab 464F → → ← Yes 1

Locker 464Q → Decontam 464M → → → Yes

Decontam 464M → Lab 464L See note See note ← No 2

Lab 464G → Lab 464F → → → Yes

Lab 464G → Animal Room 464H Neutral Neutral → Yes

Lab 464G → Animal Room 464J Neutral → → Yes

Lab 464G → Lab 464L → → → Yes

Notes

1 - temp diff, cooler in F

2 - temp diff, cooler in L, sup diff above door in L blowing air down

1.5 - Directional AirflowThe purpose of this test is to verify that there is directional airflow not only when the doors in the BSL-3 containment barrier are

closed, but also when the doors are opened.

Factors that can effect these results are temperature control between spaces, location of supply and exhaust diffusers and

registers, etc. May need to increase the offset for the zone to correct issues.

Test Procedure:

1. If door opens into the higher risk area, stand inside higher risk space, outside path of air and verify airflow direction with smoke

bottle. If door opens into the lower risk area, stand inside higher risk space, outside path of air and verify airflow direction with

smoke bottle. It is important to try and minimize the effect your body will have on the turbulence of the airflow.

2. Try to get as close to the plane of the door closed position, not where the door travel path is.

3. Test at the bottom, middle, then top of the door plane area. Note any reversals.

Reference Direction ToDoor plane area Does the door close

and latch on its own?Notes




Lexington, KY

1.6 Test Procedure

1Room under negative pressure, meaning HVAC systems; AHU, EF, air terminal devices under normal

operation at design conditions. Record the room DP values if different than section 1.4.

2

Using smoke generating device (smoke bottle, low volume), check all room penetrations, including but not

limited to: Plumbing, life safety, BAS, electrical, telecom, seams and joints (including dissimilar materials

and material transitions).

3Document all room integrity issues in the test report section (section 1.7), email to all parties. Use blue

painters tape to identify all issues in the rooms.

4 After repairs are made by owner staff/contractors, back check and update test report

5

Upon completion of all Integrated Systems Tests (IST), perform a visual inspection to confirm the secondary

containment barrier has not been compromised.

ISTs involve the failure of the HVAC and electrical systems. This can stress the sealing of the space

penetrations due to momentary, higher than normal room pressures.

1.6.1 Requirements for all sealing

Finishes, penetrations & caulking integrity for architectural elements such as door frames, around the

ceilings, lighting fixtures, electrical devices, etc. within containment to meet requirements for:

Clean-ability of all surfaces including furniture,

Smoothness of all surfaces,

Sealed seams and penetrations,

Monolithic, slip resistant floors,

Surface impermeability to liquids,

Resistance of surfaces to chemical (organic solvents, acids, alkalis), disinfectants and moderate heat,

Gas tightness for decontamination,

Pest management requirements,

Non-operable windows,

Bioseals.

1.6.2 Sealant type

NIH DRM, Appendix L – Sealant table

https://www.orf.od.nih.gov/TechnicalResources/Documents/DRM/DRM1.503262020.pdf

Refer to facility construction drawings and specifications


https://www.orf.od.nih.gov/TechnicalResources/Documents/DRM/DRM1.503262020.pdf



Lexington, KY

Room Name Room # Leak # Description of leak or integrity issue Repaired?

Ante 464 1 Supply diffuser has come away from ceiling. Diffuser is rusty

Ante 464 2 Door kick plate to 464Q is rusty

Ante 464 3Corner of vynil flooring by door frame to ante room, bottom right coming away,

open to concrete

Ante 464 4 Ceiling paint bubbling and cracking between supply diffuser and access panel.

Ante 464 5 Crack in ceiling by corner of access panel, marked VV, corner by A and corridor

Locker 464A 6 Ceiling paint bubbling and cracking between supply diffuser and access panel.

Locker 464A 7 Door that was removed, the door frame, hinges (3) and latch not sealed, leaks

Locker 464A 8 Light fixture leaks in room to D, recessed circular

Locker 464A 9Wood board mounted behind metro rack and plastic cabinet, not treated,

pourous, will be hard to decontaminate

Toilet 464B 10 Sink plumbing escutcheons sealing pulling away from wall

Toilet 464B 11 Toilet seal to wall, on bottom, no caulking/ gap

Decontam 464D 12 Electrical outlet leaks, by wall wire rack and door to F

Decontam 464D 13 Paint peeling and cracking by access panel in ceiling, close to shower

Decontam 464D 14 Access panel has metal tape, compromised seal?

Lab 464F 15 Fire alarm annunciator leaks, dirt trace visible on wall

Lab 464F 16 Emergency shower pull handle is rusty, ceiling penetration leaks

Lab 464F 17 Electrical outlet leaks, behind freezer, on wall by D

Lab 464F 18 Light switch leaks, by door to G

Lab 464F 19 Electrical outlets under cabinets, above work bench sink (3 total) leak

Lab 464F 20Area under the sink, connected to ceiling wall above? Can feel airflow, smoke

movement.

Lab 464F 21 Crack in ceiling, corner by windows, on right side

Lab 464F 22Crack in caulking on aluminum window frame and ceiling joint, middle, corners,

side areas. Top window and bottom window joints should be re-sealed.

Lab 464F 23 Area under and behind the fume hood, can feel airflow, smoke movement.

Lab 464F 24Wall paneling (wainscoating) by door to D and behind BSC B2, not sealed, seems

pourous

Lab 464G 25 Light switch leaks, by door to airlock

Lab 464G 26

Steam lines, multiple, on wall to airlock and ante room have peeling paint,

insulation coming apart, capture hood ceiling penetration has gaps, bioseal to

airlock has some gaps to wall, compressed air line ceiling pentration leaks,

recommend installing autoclave on the ante room side, so it can be serviced

outside of the BSL-3, lab G

Lab 464G 27 Screws missing in cover for the time clock for the lighting control for room H.

Lab 464G 28Floor seam by airlock has come apart, also corner on wall to airlock has come

apart, both need to be re-sealed, open to concrete.

Lab 464G 29 Paint peeling and cracking by access panel in ceiling, close to L

Lab 464G 30

Some of the access panels are missing hardware to keep them closed,

compromised seal, metal tape used to seal them. Recommend investigating

replacing.

Animal Room 464H 31 All 4 walls have cracking and peeling of paint

1.7 - Room Integrity Test Report




Lexington, KY

Animal Room 464H 32 Pipe penetration for AWS, from J, leaks, has dirt trace on wall

Animal Room 464H 33 Crack in ceiling between access panel and supply diffuser

Animal Room 464H 34 Supply diffuser is rusty

Animal Room 464H 35Access panel frame has come apart from ceiling, also has metal tape, some

hardware missing to completely close

Animal Room 464J 36 Electrical outlet above sink leaks

Animal Room 464J 37 Electrical outlet behind BSC, by wall to L, low, grey, leaks

Animal Room 464J 38 Electrical outlet behind BSC, by wall to L, high, red, leaks

Animal Room 464J 39Paint peeling and chips missing by access panel, access panel has metal tape, is

this seal working?

Animal Room 464J 40 Crack in ceiling by sprinkler head

Animal Room 464J 41 All 4 walls have cracking and peeling of paint

Lab 464L 42 Light switch by door to G leaks

Lab 464L 43Gaps in conduit ceiling penetrations for the air monitor/Sentry panel, above door

to G

Lab 464L 44Cracks in ceiling by access panel and wall, and to light fixture, above door to G

Lab 464L 45Area under the sink, connected to ceiling wall above? Can feel airflow, smoke

movement.

Lab 464L 46 Emergency shower pull handle ceiling penetration leaks

Lab 464L 47 Wall paneling (wainscoating) by door to M not sealed, seems pourous

Lab 464L 48 Holes need to be sealed, large chip in paint, on wall behind the BSC, close to M

Lab 464L 49Electrical raceway, by door to M, has leaks, can see dirt trace on wall by door

frame

Lab 464L 50 Crack in ceiling between access panel and light fixture, by door to M

Lab 464L 51 Cracks in vynil flooring, by BSC and window

Lab 464L 52Crack in caulking on aluminum window frame and ceiling joint, middle, corners,

side areas. Top window and bottom window joints should be re-sealed.

Lab 464L 53Access panels have metal tape, are the seals not working? Need to be replaced?

Lab 464L 54 Counter shelf, under cabinets, by window, has large gap in caulking, leaks

Decontam 464M 55 Crack in wall to left of door frame to L

Decontam 464M 56 Light switch leaks, by door to L

Locker 464Q 57 Crack in ceiling by back wall close to corridor 400N

Locker 464Q 58 Light fixture leaks in room to M, recessed circular

Toilet 464P 59 Sink plumbing escutcheons sealing pulling away from wall

Airlock - 60Flooring, corners; on right by door to G was repaired, but is pulling away / on left

by door to 464 is pulling away, has large gaps

No room integrity issues found in the following rooms:

Shower 464E, Shower 464N

Small dings in walls should be repaired, re-painted; examples: Airlock, decon D/M, anteroom, AHR H/J, Lab F/L/G

Notes

Cleanliness of whole lab; floors, walls, ceiling, devices (exhaust grilles, sinks, eye wash, toilet, etc) should be cleaned

before BSL-3 work is conducted




Lexington, KY

BAS CCx BAS CCx BAS CCxAnte 464 65.5/67.5 N/A 76.2 75.4 N/A 55 62 N/A 100.0%Locker 464A 71.1/73.1 N/A 74.9 74.5 N/A 58.8 240.3 N/A 100.0%Lab 464F 69/71 N/A 68.1 68.2 N/A 69.2 62.1 N/A 41.4%Lab 464G 67.9/69.9 N/A 70 70.9 N/A 63.7 67.2 N/A 21.4%Animal Room 464H 79.5/81.5 30/70 71.5 72.7 N/A 60.7 98.1 N/A 40.8%Animal Room 464J 68.4/70.4 30/70 72.4 74.1 N/A 58.6 115.5 N/A 0.0%Lab 464L 81.6/83.6 N/A 68.7 68.7 N/A 69.2 61 N/A 0.0%Locker 464Q (M) 70.5/72.5 N/A 72.5 71.4 N/A 63 Not on gfx N/A 100.0%

Locker 464Q is on the BAS graphics as M.OAT = 85 deg F, OARH = 71%, AHU-2 DAT = 55 deg F, DAH = 72%

Notes

Animal rooms should have RH sensor for monitoring and record keeping purposes.

SensorTemp RH DAT (as found)

HWV closed

(from BAS

gfx)

1.8 - Temperature and Relative Humidity (RH) sensor verification

Record the following:

1. Temp, RH and DAT from the BAS graphics.

2. Using the BK and infrared temp gun, measure the as found/ normal operation values of the temp, RH and DAT.

The temp setpoint in heating mode is listed first, cooling mode is second (example: 68/74) Each mode has a tolerance of

+/- 2 deg F.

Room Name Room #Setpoint

Temp RH




Lexington, KY

Room Name Room #

Sink hot, cold and

drain functions (if

applicable)

Is the sink hands free?

If yes, how is it

activated?

Eye wash functions?

Emergency

Shower

functions?

Floor drain

Ante 464 N/A N/A N/A N/A N/A

Locker 464A N/A N/A N/A N/A N/A

Toilet 464B Yes Manual N/A Toilet works 4"

Shower 464E Shower, no sink 1 faucet, mixer N/A N/A 3"

Decontam 464D N/A N/A N/A N/A N/A

Lab 464F No, batteries? Yes, electronic eye Yes Did not test 6"

Lab 464G N/A N/A N/A N/A

6" and

autoclave

drain

Animal Room 464H Yes Yes, electronic eye Yes N/A 5", clogged

Animal Room 464J No, batteries? Yes, electronic eye No N/A 5", clogged

Lab 464L No, batteries? Yes, electronic eye Yes Did not test 6"

Locker 464Q N/A N/A N/A N/A N/A

Shower 464N Shower, no sink 1 faucet, mixer N/A N/A 3"

Decontam 464M N/A N/A N/A N/A N/A

Toilet 464P Yes Manual N/A Toilet works 4"

Air Lock - N/A N/A N/A N/A N/A

List chemical used

464F, 464J and 464L, electronic eye not functioning

464J has AWS on wall, not used, remove all equip, patch holes, cap service lines

Where do the floor drains go? How is it treated?

Hose bib in room: 464B, 464P (toilet)

Describe how the liquid water waste is treated at sink drains

1.10 - Plumbing and Life Safety

Test procedure (As applicable) :

1. Verify that the hot and cold water lines are piped correctly at all sinks, that the sink drains properly, and there are no leaks.

2. Verify that the eye wash water is at the desired temperature (~85 deg F), it is clean and clear (no residue), can sustain the required duration

(10 min) for the flush time.

3. Verify that the emergency shower is at the desired temperature (~85 deg F), it is clean and clear (no residue), can sustain the required

duration (10 min) for the flush time.

Notes

Brandy stated that the batteries need to be changed on the sink eye, was a bi monthly maintenance item

Liquid Waste Treatment




Lexington, KY

Room Name Room #Outlets

Function?

GFCIs

Function?

Light

Fixture

Qty:

Bulbs per

fixture:

All

fixtures

working?

Switches

Functional?

Lighting

Control

System?

Notes

Ante 464 Yes N/A 1 6 Yes Yes N/A 1

Locker 464A Yes N/A 1 2 Yes 1 sw, yes, red N/A

Toilet 464B Yes Yes 1 2 Yes 1 sw, yes, red N/A

Shower 464E Yes N/A 1 2 Yes 1 sw, yes, red N/A 2

Decontam 464D N/A N/A 2 1 Yes 1 sw, yes, red N/A

Lab 464F Yes N/A 4 2 or 3 Yes 1 sw, yes, red N/A

Lab 464G Yes Yes 2 2 Yes 1 sw, yes, red N/A

Animal Room 464H Yes N/A 4 3 Yes2 sw, yes, 1 red

(left), 1 grey (right)

Yes, Tork

time clock in

464G

3

Animal Room 464J Yes N/A 4 3 Yes2 sw, yes, both are

red

Yes, Tork

time clock in

464G

4

Lab 464L Yes N/A 4 2 or 3 Yes 1 sw, yes, red N/A 5

Locker 464Q Yes N/A 1 2 Yes 1 sw, yes, red N/A

Shower 464N Yes N/A 1 2 Yes 1 sw, yes, red N/A 2

Decontam 464M N/A N/A 2 1 Yes 1 sw, yes, red N/A

Toilet 464P Yes Yes 1 2 Yes 1 sw, yes, red N/A

Air Lock - N/A N/A 1 2 or 3 Yes 1 sw, yes, red N/A 5

N/A N/A 1 1 Yes 1 sw, yes, red N/A 6

N/A N/A 1 1 Yes 1 sw, yes, red N/A 6

Room between A and D

Room between Q and M

1 - Pendant (hanging fixture)

2 - waterproof cover on switch

3 - Switches located in 464G, no red film on window on either window for H or J

Notes

5 - few bulbs may be out

6 - Recessed can, not sealed, can see through

4 - Switches are backwards, left does right bank, right does left bank

1.11 - Electrical

Test procedure:

1. Verify that all electrical outlets in the space have power using circuit tester for 120VAC outlets (open ground, open neutral,

open hot, hot/ground reverse, hot/neutral reverse). If the outlet is a GFCI type, use the test function on the switch to verify that

it is wired correctly.

2. During the power failure test (IST) verify that all electrical outlets and light fixtures that are supposed to be on the electrical

backup emergency generator have power. See electrical drawings for details.

3. Verify that all light bulbs are lit when switched on, switch shuts off all bulbs




Lexington, KY

Room Name Room #

Do

or

mat

eri

al

Latc

h

Ha

nd

le

Lock

Clo

ser

Flo

or

Swe

ep

Fram

e

Seal

Kic

k P

late

Car

t

Bu

mp

er

Inte

rlo

ck

sid

e d

oo

r?

Lite

?

Notes

Ante Room 464 Metal Yes Yes

Yes, has

manual

inside

Yes No No No No No Yes Yes to corridor 400N

Ante Room 464 Wood Yes YesCore

removedYes No No Yes No No No No to 464A


removedYes No No Yes No No No No to 464Q


removedYes No No Yes No Yes Yes Yes to airlock

Airlock - Wood Yes YesCore

removedYes No No Yes No Yes Yes Yes to 464G

Shower 464E Wood N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A two (2) shower curtains

Decontam 464D Wood Yes YesCore

removedYes No No No No No No No to lab 464F

Decontam 464D Wood Yes YesCore

removedYes No No Yes No No No No to locker 464A

Shower 464N Wood N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A two (2) shower curtains

Decontam 464M Wood Yes YesCore

removedYes No No No No No No No to lab 464L

Decontam 464M Wood Yes YesCore

removedYes No No Yes No No No No to locker 464Q

Lab 464G Wood Yes YesCore

removedYes No No No No No Yes Yes to 464 F

Lab 464G Wood Yes Yes Key YesOnly on side

doorNo No No No Yes Yes to 464H


removedYes

Only on side

doorNo No No No Yes Yes to 464J


removedYes No No No No No Yes Yes to 464L

Locker 464A removed

Locker 464Q removed

1.12 - Architectural - Doors and hardware

Notes

464 - has an occ sensor in front of door to exit to corridor

464D to 464A - door handle in D to A, on D side sticks down

Test procedure (as applicable ):

1. Record the door hardware; latch, handle, lock, closer, floor sweep, frame seal(s), etc.

2. Note any issues with door hardware, is it damaged or not performing?

Toilet 464P - This door stick, hits frame, swell? When you try to open or close




Lexington, KY

Room Name Room #Access control

typeInterlocked with?

List other security

measures and

equipment

Notes

Ante 464Card reader, PIN

padN/A N/A

Locker 464A N/A N/A N/A

Shower 464E N/A N/A N/A

Decontam 464D N/A N/A N/A

Lab 464F N/A N/A N/A

Lab 464G N/A N/A N/A

Animal Room 464H N/A N/A N/A

Animal Room 464J N/A N/A N/A

Lab 464L N/A N/A N/A

Locker 464Q N/A N/A N/A

Shower 464N N/A N/A N/A

Decontam 464M N/A N/A N/A

Air Lock - N/A

Airlock to 464

interlocked with door

from Airlock to 464G

N/A

1.13 - Access Control & SecurityTest procedure (as applicable ):

1. Verify that the access control (card reader, PIN pad, biometrics scanner, etc.) is setup and functional, by challenging without the

necessary means.

2. Verify which doors are interlocked (cannot be open at the same time) with each other, challenge the door interlock system by

trying to open both doors at the same time.

3. Note any issues with access control and/ or security system, is it damaged or not performing?




Lexington, KY

Room Room # Tag #

Any issues

closing the

BSD?

Smoke test

passed

when BSD

is closed?

Smoke test

around the

flange of

the BSD to

check for

leaks.

Any issues

opening the

BSD?

No added

flow

restrictions

(in the

open

position)?

Close

time

Mag

gauge

pressure

value

Open

timeNotes

Decon, Locker, Shower 464M, N, Q Supply Damper 1 No No N/A No No 915 1.2" 916

Lab 464L Supply Damper 2 No No N/A No No 918 1.5" 922 Can feel cold air from both diffusers, smoke disappears

Ante 464 Supply Damper 3 No No N/A No No 924 1.0" 925

Lab 464G Supply Damper 4 No No N/A No No 926 1.5" 933

Can feel cold air from both diffusers, smoke shoots down, close then

open exh damper G at 928, then close sup D 4,

Tried closing exh D G, same result

Decon, Locker, Shower 464A, C, E Supply Damper 5 No No N/A No No 935 0.85" 938Tested with exh D open, then closed, air flows out of D diffuser, A

diffuser seems OK

Lab 464F Supply Damper 6 No No N/A No No 941 1.65" 945

AHR 464H Supply Damper 7 No No N/A No No 946 1.25" 951Switch is backwards in panel;

switch closed = damper open, switch open = damper closed

AHR 464J Supply Damper 8 No No N/A No No 952 0.5" 956When switch was closed, green light stayed on indicating open, mag

gauge changed from 0.0" to 0.5".

Locker 464Q Exhaust Damper A No No N/A No No 958 3.1" 1000

Decon, Shower 464M, E Exhaust Damper B No No N/A No No 936 peg, 4"+ 938

Lab 464F Exhaust Damper C No No N/A No No 942 peg, 4"+ 945closed/ decon red light burned out, not illuminated when in closed

position

Lab 464G Exhaust Damper D No Yes N/A No No 930 peg, 4"+ 933 with sup D 4 closed

AHR 464J Exhaust Damper E No No N/A No No 954 peg, 4"+ 956

AHR 464H Exhaust Damper F No No N/A No No 949 peg, 4"+ 951

Lab 464L Exhaust Damper G No No N/A No No 1001 1.25" 1003

See room DP changes from DP array

AHU-2 SASP = 1.5"

Tested multiple times, with associated exh open and closed, same results

Could be not closing all the way, or the gasket material is compromised, but the isolation dampers are not completely closing off when commanded closed.

The Purge key switch in Corridor 400N, how does this function? I was not able to test this.

Description:

Manual Bio Seal Dampers are used to isolate the BSL-3 / ABSL-3 airflows (supply and/ or exhaust) from non-containment areas and equipment. A typical scenario would be during a decontamination of the BSL-3 while the systems

serving the containment spaces are still operational and the non- containment areas are still occupied.

1.14 - Manual / Automatic Bio Seal Damper (BSD) - Isolation Dampers

Test procedure:

1. With all HVAC air systems in normal operation

2. Close the manual BSD

3. Verify that no air is passing by in the room

4. Verify that the connection to the ductwork/flange does not leak

5. Open the manual BSD

Verification method:

a - Flowhood / flow meter (TAB)

b - Smoke generating device




Lexington, KY

Tag

De

sign

BA

S (fdb

k)

Dam

pe

r

Po

s

Tag #s

De

sign

BA

S (fdb

k)

Dam

pe

r

Po

s

De

sign

BA

S

Ante 464 200 Phx 250 Phx 0 -50Locker 464A 165 Phx 165 Phx 0 0Lab 464F 1051 Phx 829 Phx 0 222Lab 464G 760 Phx 760 Phx 0 0Animal Room 464H 336 Phx 386 Phx 0 -50Animal Room 464J 430 Phx 480 Phx 0 -50Lab 464L 91 Phx 1060 Phx 0 -969Decontam 464M 165 Phx 165 Phx 0 0Shower 464E 0 0Decontam 464D 0 0Locker 464Q 0 0Shower 464N 0 0

Air Lock - 0 0

Toilet 464BToilet 464P

Notes

No data on graphics for 464B, 464E, 464D, 464Q, 464N, 464P and Airlock464L - Supply and exhaust values do not appear to be correct on BAS gfx

1.15 - Airflows (Design CFM from schedule on drawing )

Record the following:

1. Airflow values from the BAS graphics to verify they match the design CFM values.

2. Damper position from the BAS graphics to ensure the device is under control and not operating at 100%

open position.

Room name Room #

Supply Exhaust (Total) Offset




Lexington, KY

L W HAnte 464 115 141 107 1735005 1728 1004 60 250 14.94Locker 464A 172 88 107 835456 1728 483.5 60 165 20.48Toilet 464B 70 54 107 404460 1729 233.9 60 0.00 1Decontam 464D 239 104 107 876914 1728 507.5 60 0.00 2Lab 464F 251 124 108 3361392 1728 1945 60 1050 32.39Lab 464G 174 459 107 3233433 1728 1871 60 760 24.37Animal Room 464H 126 124 107 1671768 1728 967.5 60 386 23.94Animal Room 464J 126 125 107 1685250 1728 975.3 60 480 29.53Lab 464L 252 118 108 3211488 1728 1859 60 1060 34.22Locker 464Q 172 88 107 835456 1728 483.5 60 0.00 1Toilet 464 70 54 107 404460 1729 233.9 60 250 64.12Decontam 464M 239 104 107 876914 1728 507.5 60 165 19.51 2Air Lock - 49 51 107 267393 1728 154.7 60 0.00 1

INCHES Total

(in3)

in3/ft

3

Total

(Ft3)

Notes

Includes shower areaNo data on graphics for 464B, 464E, 464D, 464Q, 464N, 464P and Airlock

1.16 - Air Changes per Hour (ACH)

The calculated ACH is important to know, example: How long to expect for containment clearance in the event of a spill, or

after a decon of the space.

Perform the following:

1. Record the room dimensions (measure the actual length, height, and width).

2. Note what CFM value was used (supply or exhaust) and from what source (Design, BAS, TAB, etc.).

Typically the highest CFM value should be used.

min/hrBAS fdbk,

airflowACHRoom Name Room # Note



PV Report - 2021


Integrated System Tests

(Failure Scenario Testing)

Integrated System Tests University of Kentucky


Lexington, KY

Rev # Issue Date Issued By / Initials Revision Description

1 2021-07-19 Cornerstone Cx / AT Initial Draft

Test Control

Test Session # Test Date Performed By Test Description

1 2021-07-27 Cornerstone Cx / DC IST P1

2 2021-07-28 Cornerstone Cx / DC IST 1, 2, 3

Loss of Normal Electrical Power Service (IST #3)

During a loss of normal electrical power, the automatic transfer switches (ATS) shall detect this loss, and switch

to emergency power generator.

Upon normal power restoration, ATS shall switch back to normal power. During both of these events, the BAS

and HVAC systems will prevent reversals of airflow in the BSL-3.

Document Control

Test Description

This section lists the specific Integrated System Tests (IST) to be performed as required for BSL-3 laboratories.

The ISTs will be performed while monitoring the differential pressure (DP) across all of the doors in the BSL-3

secondary containment barrier.

Steady state operation - Room DP - AHU, EF, air terminal units (IST Pre-test 1)

Confirm that the Supply fans in the Air handling units, the Exhaust fans, and the air terminal units/ air valves (as

applicable) serving the BSL-3 area provide steady room differential pressure (DP), this will confirm that there

are no control tuning issues, with minimal oscillations that could effect the outcome of the failure testing in

IST#1-3.

HVAC systems failure testing (IST #1 and 2)

Simulate failures of the Supply and Exhaust fans serving the BSL-3. During these failure scenarios, the BAS and

HVAC systems will prevent reversals of airflow in the BSL-3 secondary containment barriers.

Introduction

Cornerstone Commissioning, Inc. has developed the following series of Integrated System Tests (IST) to ensure

that Building Automation System (BAS), Mechanical and Electrical systems will prevent reversal of airflows in

the BSL-3 laboratory during failures scenarios described herein to comply with industry standards and

guidelines, including but not limited to; Facility design intent document, The Center for Disease Control (CDC) /

National Institutes of Health (NIH) Biosafety in Microbiological and Biomedical Laboratories (BMBL) 6th edition.




Lexington, KY

Reference




Biosafety Level 3


9. A ducted mechanical air ventilation system is required. This system provides sustained directional airflow by

drawing air into the laboratory from “clean” areas toward “potentially contaminated” areas. The laboratory is

designed such that under failure conditions the airflow will not be reversed at the containment barrier.

Test Results Executive Summary

IST #P1 - Steady state operation - Testing revealed minimal variations in room differential pressures for extended

durations.

IST #1 - AHU Supply Single Fan Failures - Testing showed no sensed reversals in the containment barrier.

IST #2 - Single Exhaust Fan Failures - Testing showed no sensed reversals in the containment barrier. There are a few

rooms that go up to neutral and the magnitude of the negative pressure decreases. The exhaust fans do not have

redundancy.

IST #3 - Electrical Power Failure and Restoration - Testing showed no sensed reversals in the containment barrier. 464L

does go positive to 464G and remains that way until normal power is restored. This could be related to 464H

overcoming the pressure of 464L, and the air handling unit (AHU-2) being off.





Lexington, KY

Verification

To verify there is no reversal of airflow, Cornerstone Commissioning, Inc. will use a multi room differential pressure

(DP) array to measure, monitor and record the differential pressure values at critical points of containment in the BSL-

3 zones during the failure scenario tests. The data generated from the DP array will help determine if the IST passes or

fails.

The DP array is independent of the building automation system.

DP array sensors range = +/-0.25” wc with 0.25% full scale accuracy. This is the appropriate range for the expected

room pressures.

DP array data logger recording interval = 500 milliseconds (2 readings per second).

The equipment is setup to capture any excursions or potential airflow reversals across the monitored doors with the

accuracy and speed required.

Smoke bottles and hand held micromanometers may also be used to confirm directional airflow during these tests.

A reversal is defined as a value beyond neutral. Neutral is an acceptable condition. The goal is to prevent sustained

reversals to limit the risk of a failure of the secondary containment barrier.

Pass Fail Criteria for IST #P1

The supply and exhaust room pressure control is critical to the safe operation of the BSL-3 laboratory.

Tuning for the zone level airflow control loops is critical to provide steady room pressures.

The zone level airflows for BSL-3 shall be tuned so that the normal room pressure operating value does not vary by

more than 25% for any duration.

This pretest will confirm that controls contractor and/or laboratory airflow control vendor has tuned the control loops

for the supply and exhaust airflow control so that the room pressure does not vary by more than 25% of the operating

value for any duration.

Example would be, desired room DP to be determined during commissioning, setup range between -0.03" to -0.09"

wc, -0.06" mid point, no oscillations above or below by 25% of the final room DP value.

Pass Fail Criteria for IST#1-3 (Suggested )

The following is the pass fail criteria for all of the doors in the BSL-3 laboratory secondary containment barrier during

the failure mode testing:

If there is a sensed reversal of greater than +0.05” wc for a time of more than 10 seconds across any door.

If there is a sensed reversal across two consecutive doors of any magnitude for longer than 5 seconds.

Cornerstone Cx considers these values to be a reportable incident once the laboratory is “hot”.

A risk assessment will be done if the criteria is not met to determine the course of action with consideration to the

scenario, the specific door, the magnitude and duration of the reversal, path from PECs, and the SOPs.

Commissioning Test Equipment

Smoke bottle (Regin) http://www.regin.com/product/fp201-low-velocity-powdered-smoke/

Micromanometer (Fluke 922)

DP Array - v7 - Ten Channel - Ashcroft - RXLDP +/- 0.25 in wc sensors




Lexington, KY

Tag # Description

AHU-2 Air Handling Unit with two (2) supply fans

SAV Phoenix Controls air valve

Tag # Description

EF-1 Exhaust Fan

EF-2 Exhaust Fan

N/A HEPA filter housing

EAV Phoenix Controls air valve

Drawing #: Airflow diagram

Drawing is attached at end of this document

HVAC equipment

Supply air systems and equipment

Exhaust air systems and equipment

HVAC One line diagram




Lexington, KY

Tag # Description

ATS-1 Automatic Transfer Switch

G-1 Generator

MB Main circuit breaker

Drawing #: Not available

Electrical equipment

Electrical One line diagram




Lexington, KY

Room Name Room # Room Name Room #

1 Corridor 400N → Ante Room 464

2 Ante Room 464 → Airlock no #

3 Airlock no # → Lab 464G

4 Lab 464G → Lab 464F

5 Lab 464G → Animal Room 464H

6 Lab 464G → Animal Room 464J

7 Lab 464G → Lab 464L

8 Decontam 464D → Lab 464G

9 Decontam 464M → Lab 464G

Differential Pressure monitoring locations

Reference (Low - ) To (High + )

BSL-3 points of containment (doors) to be monitored during ISTs:

Airflow

Direction

DP Array

Channel #




Lexington, KY

Floorplan - Differential Pressure locations for reference




Lexington, KY

# Test Prerequisites - Description Status

1 All equipment and system performance verification tests are complete. Complete

2 Verified room airflow and pressure control devices with accuracy and operation. Complete

3 Steady redundant supply and exhaust airflows (review trends). Complete

4 If the facility has HEPA filters, they must be installed, tested, and certified. Complete

5 All BSCs must be certified and operational. Complete

6All systems/ equipment are in normal (automatic) operation with no overrides in the BAS or lab control system.

Complete

7 All doors in the building are closed. Complete

8All surrounding non containment areas and equipment in normal operation, as they would be when the building is in

use by the owner.Complete

9 All alarms are clear for the containment area. Complete

10 Containment area is closed to all personnel not involved with ISTs. Complete

11Plumbing drains are wet, dry traps can cause issues with room DP. Check all sinks, showers, and toilets, as applicable.

Complete

12

Setup the Differential Pressure (DP) Array to measure containment points in the containment zones for pressure

readings during the tests. This will determine if the IST passes or fails. (See DP array setup checklist below). Complete

13Confirm with the Owner staff that all building occupants that could be affected by the failure tests have been

notified?Complete

14Review with Owner staff (Lab manager, EH&S, researchers, etc. ) all doors in secondary containment barrier that are

being monitored.Complete

15Review with Owner staff (Lab manager, EH&S, researchers, etc. ) pass/ fail criteria, confirm before testing starts.

Complete

# Item Status

1 Verify that each sensor zero is within tolerance (0.000" wc), before connecting the tubes to the sensors Complete

2 Verify that each sensor reads within range (+/-0.25" wc) Complete

3 Ensure DP Array measured values are within tolerances of NIST calibrated meters Complete

4 Open each door being monitored to ensure it is correctly indicated on the DP array Complete

5 Verify that the polarity is correct for each sensor Complete

6 Confirm laptop used to record data has the correct date and time, for the area/ time zone that you are in. Complete

7Ensure that the UPS that the DP array is plugged into is functioning, by unplugging from the 120VAC electrical outlet,

this will ensure the battery in the UPS is functional.Complete

8When using the DP array, during testing, set the display time interval to 5 min, so you can see any oscillations

Complete

Prerequisites

This section lists pre-requisites to be completed and approved prior to performing the test.

DP Array setup checklist - To confirm the DP Array is working correctly

This should be done at the start of each testing session after all tubing is run.




Lexington, KY

# Event Status

1 All personnel involved in test meet to review expectations Complete

2

Confirm that there are no safety concerns from any personnel involved. Confirm that there will be no harm to

personnel, equipment or the facility. Confirm that all personnel will use appropriate personal protective equipment

(PPE) for their required actions during the tests and that Cornerstone Cx assumes no responsibility to damaged

equipment, systems or the facility.

Complete

3 Personnel go to assigned locations Complete

4 Confirm DP Array and all tubing setup per pre req Complete

5 Communication check, ensure all personnel have completed pre-test tasks Complete

6Record the as found, normal operating parameters for the HVAC systems and equipment that serve the BSL-3 at the

start of each testing sessionComplete

7Ask BAS operator to capture screenshot of the BAS graphics indicating normal operation of the systems and that

there are no active alarms.Complete

8When using the DP array, during testing, set the display time interval to 5 min (on the connected laptop and the data

logger), so you can see any oscillations or anomolies.Complete

Day of test prerequisites

This should be done at the start of each testing session .




Lexington, KY

Personnel Location Task

Owner staff Mech / elec spaces Observe system operation.

Owner Mech staff Mech spaces Equipment VFDs to simulate fan failures (via the power

disconnect).

Owner Elec staff Elec spaces Open and close main electrical power source/ breakers.

Generator rep/ Owner staff Generator Ensure the generator performs as expected. Can

troubleshoot and address any issues encountered.

Owner BAS staff Hard wired into control

system. Wifi is not reliable

during power failure tests

unless on UPS.

Reset equipment, capture screenshots of equipment

parameters, alarms, etc. Can troubleshoot and address

any issues encountered.

Equipment vendor/rep or

Owner BAS staff

Lab control system access

point

Can troubleshoot and address any issues encountered.

Owner staff BSL-3 Laboratory Observe operation of laboratory systems and equipment.

Dan Cook BSL-3 Laboratory Direct test events.

Monitor BSL-3 room pressures, review trend data.

Personnel - Required Participants, Locations and Tasks




Lexington, KY

Date: 2021-07-27

-

Confirm that the Supply fans in the Air handling units, the Exhaust fans, and the air terminal units/ air valves (as applicable) serving

the BSL-3 area provide steady room differential pressure (DP), this will confirm that there are no control tuning issues, with

minimal oscillations that could effect the outcome of the failure testing in IST#1-3.

Record the room DPs with all systems in normal operation, no personnel inside the containment barrier, no entry or exit, for 5

minutes. This will establish the room pressure baseline for comparison during the failure scenarios.

# Event

1 Enter the start time, when recording was started on the DP array.

2 Enter the stop time, when recording was stopped on the DP array.

3 Using the DP array CSV file, generate graph and data analysis table.

4Review results, if there are no anomalies and the systems and equipment provide steady room DP, then proceed to next failure

test/ integrated system test.

Time Event

1600 Start recording

1605 Stop recording

IST #P1 - Steady State Operation - Room DP - AHU, EF, air terminal units

Conclusion

Review of room DP indicates that the AHU, EF, air terminal units are operating in a manner that provides steady room DP values that

do not oscillate by more than 20% of average pressure reading.

Event Log

Test Script

Test Description


IST P1 - UK HSRB 4th flr BSL-3 - 2021-07-27_1546_All events


-0.16

-0.14

-0.12

-0.1

-0.08

-0.06

-0.04

-0.02

0

16

:00

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16

:04

:55

Corridor 400N/Ante Room 464 Ante Room 464/Airlock no # Airlock no #/Lab 464G

Lab 464G/Lab 464F Lab 464G/Animal Room 464H Lab 464G/Animal Room 464J

Lab 464G/Lab 464L Decontam 464D/Lab 464G Decontam 464M/Lab 464G

IST P1 - UK HSRB 4th flr BSL-3 - 2021-07-27_1546_Data Analysis

Data and Graph event: All Events - 5 minute steady state

Start time: 16:00:00

End time: 16:05:00

5 minute steady state 1 2 3 4 5 6 7 8 9

Room DP monitored

Corridor

400N/Ant

e Room

464

Ante

Room

464/Airloc

k no #

Airlock no

#/Lab

464G

Lab

464G/Lab

464F

Lab

464G/Ani

mal Room

464H

Lab

464G/Ani

mal Room

464J

Lab

464G/Lab

464L

Decontam

464D/Lab

464G

Decontam

464M/Lab

464G

Min value -0.0603 -0.0225 -0.0259 -0.0563 -0.0422 -0.1377 -0.0252 -0.0593 -0.0564

Max value -0.0458 -0.0181 -0.0206 -0.0484 -0.0391 -0.1336 -0.0188 -0.0515 -0.0477

Average value -0.0535 -0.02046 -0.02369 -0.05264 -0.04081 -0.13586 -0.02193 -0.05495 -0.05192

Avg/Min % 88.7% 90.9% 91.5% 93.5% 96.7% 98.7% 87.0% 92.7% 92.1%

Avg/Max % 116.8% 113.1% 115.0% 108.8% 104.4% 101.7% 116.7% 106.7% 108.8%

Delta (Highlight if value is > 0.02") 0.015 0.004 0.005 0.008 0.003 0.004 0.006 0.008 0.009







Lexington, KY

Date: 2021-07-28

-

Simulate failures of the Air Handling Unit (AHU) supply fans serving the BSL-3.

The intent is to confirm redundancy, if there are more than one (1) fan in the system.

Note: Fan failures are unplanned events, fan restorations are planned events, the pass fail criteria should reflect the risk assessment

for this failure scenario.

During these failure scenarios, the BAS and HVAC systems will prevent a reversal of airflow in the BSL-3 secondary containment

barriers.

# Event


2 Shut off the AHU supply fan at the VFD electrical power disconnect. Note which fan below.

3Note the time and the operating parameters for the other AHU supply fan(s) when at static pressure setpoint and the room DP has

settled out.4 Ask BAS operator to capture screenshot of the BAS graphics indicating the off fan and any alarms generated.

5 Note any local alarms (Room pressure monitor, BSC, horn/strobe in the lab, etc.)

6 Note any observed airflow reversals from the DP array display and/or laptop used to record.

7 Restore the AHU supply fan at the VFD electrical power disconnect. Note if alarm or system reset is required via BAS.


9 Confirm all supply and exhaust fans are operating at parameters from the start of the test.

10 Repeat steps 2-8 for all AHU supply air fans.

11 Enter the stop time, when recording was stopped on the DP array, after all systems have settled out.

12 Collect screenshots from BAS operator.

13 Confirm that the results were captured in the DP array generated CSV/excel file.


15

If there are no anomalies and the systems and equipment prevent reversals of airflow, according to the pass fail criteria, then proceed

to next failure test/ integrated system test.

If there are anomalies and sensed reversals of airflow, review results with owner and determine best course of action, and if testing

should continue. Based on if modifications to the systems, equipment, parameters, setpoints, etc. Document all changes made and

repeat tests as necessary until favorable results are achieved.

IST#1 - Failure of the Air Handling Unit supply fans

Test Description

Test Script




Lexington, KY

Date: 2021-07-28

IST#1 - Failure of the Air Handling Unit supply fans

Time Event

656 Start recording

701 Shut off AHU-2 fan B via BAS

- AHU-3 fan A ramps to 100%

705 Restore AHU-2 fan B via BAS

- Found issue with BAS graphics and VFD settings, fan B did not restart

- Personnel walk in and out of Anteroom 464

733 AHU-2 fan A and B are both running, supply air static pressure (SASP) is at setpoint

735 Shut off AHU-2 fan A via BAS

- AHU-2 fan B ramps to 100%

739 Restore AHU-2 fan A via BAS

- Found issue with BAS graphics and VFD settings, fan B did not restart

- Personnel walk in and out of Anteroom 464

750 AHU-2 fan A and B are both running

754 SASP at setpoint

755 Stop recording

1 Eric Hess - UK Facilities has a list of items that need to be addressed in the VFD for these fans and the BAS graphics.

2 The supply and exhaust isolation dampers remained open during these failure events.

Notes

Conclusion

After reviewing the room differential pressure (DP) trend data, this test passed as there were no indications of reversal of

pressurization at the containment barrier. See attached room DP graphs and data analysis for details from test events.

Event Log


IST 1 - UK HSRB 4th flr BSL_3 - 2021-07-28_0656_All events


-0.3

-0.25

-0.2

-0.15

-0.1

-0.05

0

0.05

6:5

6:2

96

:57

:27

6:5

8:2

56

:59

:23

7:0

0:2

17

:01

:19

7:0

2:1

77

:03

:15

7:0

4:1

37

:05

:11

7:0

6:0

97

:07

:07

7:0

8:0

57

:09

:03

7:1

0:0

17

:10

:59

7:1

1:5

77

:12

:55

7:1

3:5

37

:14

:51

7:1

5:4

97

:16

:47

7:1

7:4

57

:18

:43

7:1

9:4

17

:20

:39

7:2

1:3

77

:22

:35

7:2

3:3

37

:24

:31

7:2

5:2

97

:26

:27

7:2

7:2

57

:28

:23

7:2

9:2

17

:30

:19

7:3

1:1

77

:32

:15

7:3

3:1

37

:34

:11

7:3

5:0

97

:36

:07

7:3

7:0

57

:38

:03

7:3

9:0

17

:39

:59

7:4

0:5

77

:41

:55

7:4

2:5

37

:43

:51

7:4

4:4

97

:45

:47

7:4

6:4

57

:47

:43

7:4

8:4

17

:49

:39

7:5

0:3

77

:51

:35

7:5

2:3

37

:53

:31

7:5

4:2

9

Corridor 400N / Ante Room 464 Ante Room 464 / Airlock no # Airlock no # / Lab 464G

Lab 464G / Lab 464F Lab 464G / Animal Room 464H Lab 464G / Animal Room 464J

Lab 464G / Lab 464L Decontam 464D / Lab 464G Decontam 464M / Lab 464G

Dan Cook - Cornerstone Cx

Callout

AHU-2 fan B off


Callout

AHU-2 fan B restore


Callout

AHU-2 fan A off


Callout

AHU-2 fan A restore

IST 1 - UK HSRB 4th flr BSL_3 - 2021-07-28_0656_Data Analysis (Count above 0.005" wc)

Data and Graph event: All Events

Start time: 6:56:29

End time: 7:54:57

DP Array Ch # 1 2 3 4 5 6 7 8 9

Room DP monitored

Corridor

400N /

Ante

Room 464

Ante

Room 464

/ Airlock

no #

Airlock no

# / Lab

464G

Lab 464G

/ Lab 464F

Lab 464G

/ Animal

Room

464H

Lab 464G

/ Animal

Room

464J

Lab 464G

/ Lab 464L

Decontam

464D /

Lab 464G

Decontam

464M /

Lab 464G

Average (in wc) -0.1320 -0.0434 -0.0476 -0.0262 -0.0445 -0.1372 -0.0115 -0.0712 -0.0656

Min value (in wc) -0.2713 -0.1249 -0.1288 -0.0528 -0.0576 -0.1515 -0.0507 -0.1156 -0.1114

Max value (in wc) 0.0050 -0.0002 -0.0064 -0.0146 -0.0171 -0.1263 0.0074 -0.0232 -0.0265

COUNT above 0.005 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0

Seconds above neutral 0 0 0 0 0 0 0.5 0 0




Lexington, KY

Date: 2021-07-28

-

Simulate failures of the Exhaust fans serving the BSL-3.

The intent is to confirm redundancy, if there are more than one (1) fan in the system.

Note: Fan failures are unplanned events, fan restorations are planned events, the pass fail criteria should reflect the risk assessment

for this failure scenario.

During these failure scenarios, the BAS and HVAC systems will prevent a reversal of airflow in the BSL-3 secondary containment

barriers.

# Event


2 Shut off the Exhaust fan at the VFD electrical power disconnect. Note which fan below.

3Note the time and the operating parameters for the other Exhaust fan(s) when at static pressure setpoint and the room DP has settled

out.4 Ask BAS operator to capture screenshot of the BAS graphics indicating the off fan and any alarms generated.



7 Restore the Exhaust fan at the VFD electrical power disconnect. Note if alarm or system reset is required via BAS.



10 Repeat steps 2-8 for all Exhaust air fans.





15

If there are no anomalies and the systems and equipment prevent reversals of airflow, according to the pass fail criteria, then proceed

to next failure test/ integrated system test.

If there are anomalies and sensed reversals of airflow, review results with owner and determine best course of action, and if testing

should continue. Based on if modifications to the systems, equipment, parameters, setpoints, etc. Document all changes made and

repeat tests as necessary until favorable results are achieved.

IST#2 - Failure of the Exhaust Fans

Test Description

Test Script




Lexington, KY

Date: 2021-07-28

IST#2 - Failure of the Exhaust Fans

Time Event

625 Start recording

626 EF-1 off at the VFD disconnect

- RPMs in alarm after 60 seconds

628 Personnel walk into Anteroom 464

634 Restore power at EF-1 VFD disconnect


643 Personnel walk out of Anteroom 464





651 Restore power at EF-2 VFD disconnect

656 Stop recording

- REPEAT TEST FOR EF-1 TO CONFIRM OPERATION AND PREVIOUS RESULTS

755 Start recording





- Restore power at EF-1 VFD disconnect


805 Stop recording

1 The supply and exhaust isolation dampers remained open during these failure events.

Event Log

Notes

Conclusion

After reviewing the room differential pressure (DP) trend data, this test passed as there were no indications of reversal of

pressurization at the containment barrier. See attached room DP graphs and data analysis for details from test events.


IST 2 - UK HSRB 4th flr BSL-3 - 2021-07-28_0625_All events


-0.25

-0.2

-0.15

-0.1

-0.05

0

0.05

0.1

6:2

5:4

86

:26

:18

6:2

6:4

96

:27

:19

6:2

7:5

06

:28

:20

6:2

8:5

16

:29

:21

6:2

9:5

26

:30

:22

6:3

0:5

36

:31

:23

6:3

1:5

46

:32

:24

6:3

2:5

56

:33

:25

6:3

3:5

66

:34

:26

6:3

4:5

76

:35

:27

6:3

5:5

86

:36

:28

6:3

6:5

96

:37

:29

6:3

8:0

06

:38

:30

6:3

9:0

16

:39

:31

6:4

0:0

26

:40

:32

6:4

1:0

36

:41

:33

6:4

2:0

46

:42

:34

6:4

3:0

56

:43

:35

6:4

4:0

66

:44

:36

6:4

5:0

76

:45

:37

6:4

6:0

86

:46

:38

6:4

7:0

96

:47

:39

6:4

8:1

06

:48

:40

6:4

9:1

16

:49

:41

6:5

0:1

26

:50

:42

6:5

1:1

36

:51

:43

6:5

2:1

46

:52

:44

6:5

3:1

56

:53

:45

6:5

4:1

66

:54

:46

6:5

5:1

76

:55

:47

6:5

6:1

8





Callout

EF-1 off


Callout

EF-1 restore


Callout

EF-2 off


Callout

EF-2 restore

IST 2 - UK HSRB 4th flr BSL-3 - 2021-07-28_0625_EF-1 off restore


-0.25

-0.2

-0.15

-0.1

-0.05

0

0.05

0.1

6:2

7:2

06

:27

:28

6:2

7:3

66

:27

:44

6:2

7:5

26

:28

:00

6:2

8:0

86

:28

:16

6:2

8:2

46

:28

:32

6:2

8:4

06

:28

:48

6:2

8:5

66

:29

:04

6:2

9:1

26

:29

:20

6:2

9:2

86

:29

:36

6:2

9:4

46

:29

:52

6:3

0:0

06

:30

:08

6:3

0:1

66

:30

:24

6:3

0:3

26

:30

:40

6:3

0:4

86

:30

:56

6:3

1:0

46

:31

:12

6:3

1:2

06

:31

:28

6:3

1:3

66

:31

:44

6:3

1:5

26

:32

:00

6:3

2:0

86

:32

:16

6:3

2:2

46

:32

:32

6:3

2:4

06

:32

:48

6:3

2:5

66

:33

:04

6:3

3:1

26

:33

:20

6:3

3:2

86

:33

:36

6:3

3:4

46

:33

:52

6:3

4:0

06

:34

:08

6:3

4:1

66

:34

:24

6:3

4:3

26

:34

:40

6:3

4:4

86

:34

:56

6:3

5:0

46

:35

:12




IST 2 - UK HSRB 4th flr BSL-3 - 2021-07-28_0625_EF-2 off restore


-0.25

-0.2

-0.15

-0.1

-0.05

0

0.05

0.1

6:4

5:1

0

6:4

5:1

7

6:4

5:2

4

6:4

5:3

1

6:4

5:3

8

6:4

5:4

5

6:4

5:5

2

6:4

5:5

9

6:4

6:0

6

6:4

6:1

3

6:4

6:2

0

6:4

6:2

7

6:4

6:3

4

6:4

6:4

1

6:4

6:4

8

6:4

6:5

5

6:4

7:0

2

6:4

7:0

9

6:4

7:1

6

6:4

7:2

3

6:4

7:3

0

6:4

7:3

7

6:4

7:4

4

6:4

7:5

1

6:4

7:5

8

6:4

8:0

5

6:4

8:1

2

6:4

8:1

9

6:4

8:2

6

6:4

8:3

3

6:4

8:4

0

6:4

8:4

7

6:4

8:5

4

6:4

9:0

1

6:4

9:0

8

6:4

9:1

5

6:4

9:2

2

6:4

9:2

9

6:4

9:3

6

6:4

9:4

3

6:4

9:5

0

6:4

9:5

7

6:5

0:0

4

6:5

0:1

1

6:5

0:1

8

6:5

0:2

5

6:5

0:3

2

6:5

0:3

9

6:5

0:4

6

6:5

0:5

3

6:5

1:0

0

6:5

1:0

7

6:5

1:1

4

6:5

1:2

1

6:5

1:2

8

6:5

1:3

5

6:5

1:4

2

6:5

1:4

9




IST 2 - UK HSRB 4th flr BSL-3 - 2021-07-28_0625_Data Analysis (Count above 0.005" wc)


Start time: 6:25:48

End time: 6:56:19

DP Array Ch # 1 2 3 4 5 6 7 8 9

Room DP monitored

Corridor

400N /

Ante

Room 464

Ante

Room 464

/ Airlock

no #

Airlock no

# / Lab

464G

Lab 464G

/ Lab 464F

Lab 464G

/ Animal

Room

464H

Lab 464G

/ Animal

Room

464J

Lab 464G

/ Lab 464L

Decontam

464D /

Lab 464G

Decontam

464M /

Lab 464G

Average (in wc) -0.0062 -0.0074 -0.0063 -0.0200 -0.0340 -0.1213 -0.0216 -0.0182 -0.0287

Min value (in wc) -0.0915 -0.0297 -0.0320 -0.0505 -0.1036 -0.2138 -0.1133 -0.0502 -0.0972

Max value (in wc) 0.0628 0.0152 0.0150 0.0135 -0.0200 -0.0836 -0.0018 0.0135 0.0017

COUNT above 0.005 1612.0 294.0 1071.0 2.0 0.0 0.0 0.0 860.0 0.0

Seconds above neutral 806 147 535.5 1 0 0 0 430 0

Data and Graph event: EF-1 off & restore

Start time: 6:27:20

End time: 6:35:20

DP Array Ch # 1 2 3 4 5 6 7 8 9

Room DP monitored

Corridor

400N /

Ante

Room 464

Ante

Room 464

/ Airlock

no #

Airlock no

# / Lab

464G

Lab 464G

/ Lab 464F

Lab 464G

/ Animal

Room

464H

Lab 464G

/ Animal

Room

464J

Lab 464G

/ Lab 464L

Decontam

464D /

Lab 464G

Decontam

464M /

Lab 464G

Average (in wc) 0.0163 0.0017 0.0040 -0.0049 -0.0297 -0.1074 -0.0079 0.0020 -0.0069

Min value (in wc) -0.0806 -0.0283 -0.0311 -0.0458 -0.1036 -0.2138 -0.0848 -0.0492 -0.0803

Max value (in wc) 0.0628 0.0107 0.0113 0.0135 -0.0235 -0.1001 -0.0018 0.0075 -0.0013

COUNT above 0.005 873.0 29.0 332.0 1.0 0.0 0.0 0.0 162.0 0.0

Seconds above neutral 436.5 14.5 166 0.5 0 0 0 81 0

Data and Graph event: EF-2 off & restore

Start time: 6:45:10

End time: 6:51:50

DP Array Ch # 1 2 3 4 5 6 7 8 9

Room DP monitored

Corridor

400N /

Ante

Room 464

Ante

Room 464

/ Airlock

no #

Airlock no

# / Lab

464G

Lab 464G

/ Lab 464F

Lab 464G

/ Animal

Room

464H

Lab 464G

/ Animal

Room

464J

Lab 464G

/ Lab 464L

Decontam

464D /

Lab 464G

Decontam

464M /

Lab 464G

Average (in wc) 0.0245 0.0041 0.0065 -0.0038 -0.0248 -0.0965 -0.0156 0.0060 -0.0086

Min value (in wc) -0.0915 -0.0297 -0.0320 -0.0435 -0.1009 -0.2030 -0.1133 -0.0502 -0.0972

Max value (in wc) 0.0436 0.0152 0.0150 0.0095 -0.0200 -0.0836 -0.0091 0.0135 0.0017

COUNT above 0.005 739.0 265.0 739.0 1.0 0.0 0.0 0.0 698.0 0.0

Seconds above neutral 369.5 132.5 369.5 0.5 0 0 0 349 0




-0.2

-0.15

-0.1

-0.05

0

0.05

07

:55

:05

07

:55

:15

07

:55

:26

07

:55

:36

07

:55

:47

07

:55

:57

07

:56

:08

07

:56

:18

07

:56

:29

07

:56

:39

07

:56

:50

07

:57

:00

07

:57

:11

07

:57

:21

07

:57

:32

07

:57

:42

07

:57

:53

07

:58

:03

07

:58

:14

07

:58

:24

07

:58

:35

07

:58

:45

07

:58

:56

07

:59

:06

07

:59

:17

07

:59

:27

07

:59

:38

07

:59

:48

07

:59

:59

08

:00

:09

08

:00

:20

08

:00

:30

08

:00

:41

08

:00

:51

08

:01

:02

08

:01

:12

08

:01

:23

08

:01

:33

08

:01

:44

08

:01

:54

08

:02

:05

08

:02

:15

08

:02

:26

08

:02

:36

08

:02

:47

08

:02

:57

08

:03

:08

08

:03

:18

08

:03

:29

08

:03

:39

08

:03

:50

08

:04

:00

08

:04

:11

08

:04

:21

08

:04

:32

08

:04

:42

08

:04

:53

08

:05

:03

08

:05

:14

08

:05

:24





Callout

EF-1 off


Callout

EF-1 restore



Start time: 7:55:05

End time: 8:05:26

DP Array Ch # 1 2 3 4 5 6 7 8 9

Room DP monitored

Corridor

400N /

Ante

Room 464

Ante

Room 464

/ Airlock

no #

Airlock no

# / Lab

464G

Lab 464G

/ Lab 464F

Lab 464G

/ Animal

Room

464H

Lab 464G

/ Animal

Room

464J

Lab 464G

/ Lab 464L

Decontam

464D /

Lab 464G

Decontam

464M /

Lab 464G

Average (in wc) -0.0523 -0.0170 -0.0165 -0.0174 -0.0353 -0.1276 -0.0399 -0.0289 -0.0433

Min value (in wc) -0.1362 -0.0474 -0.0472 -0.0526 -0.0879 -0.1886 -0.1329 -0.0735 -0.0824

Max value (in wc) 0.0326 0.0055 0.0069 0.0143 -0.0231 -0.0990 -0.0209 0.0137 -0.0088

COUNT above 0.005 6.0 1.0 2.0 2.0 0.0 0.0 0.0 1.0 0.0

Seconds above neutral 3 0.5 1 1 0 0 0 0.5 0




Lexington, KY

Date: 2021-07-28

-

Simulate failure of the Electrical Utility Power serving the building/ facility that includes the BSL-3 laboratory.

The intent is to document a real life loss of electrical utility and confirm the emergency electrical power systems function in a

manner that will prevent reversals of airflow in the BSL-3 laboratory.

Note: Electrical utility power failures are unplanned events, electrical utility power restorations are planned events, the pass fail

criteria should reflect the risk assessment for this failure scenario.

During these failure scenarios, the BAS, HVAC and electrical systems will prevent a reversal of airflow in the BSL-3 secondary

containment barriers.

# Event

1Confirm there is enough fuel in the generator fuel tank to get through the test for the planned duration. Record the

generator fuel tank level.

2 Confirm that the ATS(s) and generator are in in auto operation (if no abort test)

3 Record the power meter(s) value while on normal power.


5 Simulate a loss of the normal electrical utility power source by opening the main breaker.

6Confirm that the ATS senses the loss of normal power and starts the emergency generator in (sec):

(This is part of the total time to switch from normal to emergency)

7 ATS switches to emergency generator after commanding it to run in (sec):

8Observe that the ATS switches from normal power to standby emergency generator.

Record the transfer times below.

9Verify that all electrical panels that are served by the emergency generator have power. (yes/no ) Reference the

applicable Electrical drawing(s)

10Verify that all lights that are served by the emergency generator have power and are on. (yes/no ) Reference the


11Verify that all electrical outlets that are served by the emergency generator have power. (yes/no ) Reference the


12 Record any alarm or system reset(s) that had to occur to re-start equipment after the power loss.

13When all the systems that are on emergency power are back online, operating at their setpoints, and settled out,

record the time.

14Note the time when the AHU supply fan(s) and Exhaust fans are operating to achieve static pressure setpoint and the

room DP has settled out.

15Ask BAS operator to capture screenshot of the BAS graphics indicating equipment that is off and any alarms generated.



18 Let the generator run for at least 20 min before restoring normal power.

19 Record the power meter(s) value while on emergency generator after systems have settled out (kW):

IST#3 - Electrical Utility Power Failure and Restoration

Test Description

Test Script




Lexington, KY

Date: 2021-07-28


# Event

20 Restore normal power by closing the main breaker.

21Record the ATS(s) delay time (min) that normal power has to be available for it to switch from emergency power

generator.

22 Record the time that the ATS(s) switches from emergency generator to normal power.

23Record the emergency generator cooldown delay time (min) after the ATS has switched from emergency generator

power to normal power.

24 Record the time that the emergency generator goes off.




28When all the systems are back online, operating at their setpoints, and settled out while on normal power, record the

time.

29 Record the power meter(s) values when all systems are running on normal power (kW):

30 Record the Generator fuel tank level (gallons) once it is off.





35

If there are no anomalies and the systems and equipment prevent reversals of airflow, according to the pass fail

criteria, then proceed to next failure test/ integrated system test.

If there are anomalies and sensed reversals of airflow, review results with owner and determine best course of action,

and if testing should continue. Based on if modifications to the systems, equipment, parameters, setpoints, etc.

Document all changes made and repeat tests as necessary until favorable results are achieved.

Test Script




Lexington, KY

Date: 2021-07-28


Time Event

447 Start recording

458 Open normal power (NP) electrical utility feed to the building (multiple feeds)

- All eight (8) supply isolation dampers indicate closed on the control panel in Anteroom 464

- AHU-2 is off, the outside air damper will not open for this unit, reason it won't restart

520 Personnel walk into Anteroom 464525 Personnel walk out of Anteroom 464531 Normal power restored, anything not on emergency generator backup power restarts

- AHU-2 is starting534 Supply isolation damper closed = 1, 2, 4, 5 / open = 3, 6, 7545 Supply isolation damper 2 is closed, all others are open537 ATS switch from emergency generator backup power to normal power538 Generator shuts off after 1 minute cooldown

- JACE lost communication, Eric Hess stated it needs a UPS539 Supply isolation damper closed = 1, 2, 4, 5 / open = 3, 6, 7541 Supply isolation damper 2 is closed, all others are open

- JACE is communicating544 Personnel walk out of Anteroom 464545 Personnel walk from Anteroom 464 to Lab 464L through the locker, decon rooms Q & M and back to 464546 Personnel walk out of Anteroom 464549 Personnel walk into Anteroom 464552 AHU-2 and EF-1, 2 are at setpoint

- Room DP is different than at the start of test, other AHU and EF in the building are restarting.557 Personnel walk into Anteroom 464

613 Room DP is close to values at start of test


615 Stop recording

1 464L does go positive to 464G and remains that way until normal power is restored. This could be related to 464H

overcoming the pressure of 464L, and the air handling unit (AHU-2) being off.

Notes

Conclusion

After reviewing the room DP trend data, this test passed as there were no indications of reversal of pressurization at the

containment barrier. See attached room differential pressure (DP) graphs for all events.

Event Log




-0.35

-0.3

-0.25

-0.2

-0.15

-0.1

-0.05

0

0.05

0.1

0.15

4:4

7:5

14

:49

:17

4:5

0:4

44

:52

:10

4:5

3:3

74

:55

:03

4:5

6:3

04

:57

:56

4:5

9:2

35

:00

:49

5:0

2:1

65

:03

:42

5:0

5:0

95

:06

:35

5:0

8:0

25

:09

:28

5:1

0:5

55

:12

:21

5:1

3:4

85

:15

:14

5:1

6:4

15

:18

:07

5:1

9:3

45

:21

:00

5:2

2:2

75

:23

:53

5:2

5:2

05

:26

:46

5:2

8:1

35

:29

:39

5:3

1:0

65

:32

:32

5:3

3:5

95

:35

:25

5:3

6:5

25

:38

:18

5:3

9:4

55

:41

:11

5:4

2:3

85

:44

:04

5:4

5:3

15

:46

:57

5:4

8:2

45

:49

:50

5:5

1:1

75

:52

:43

5:5

4:1

05

:55

:36

5:5

7:0

35

:58

:29

5:5

9:5

66

:01

:22

6:0

2:4

96

:04

:15

6:0

5:4

26

:07

:08

6:0

8:3

56

:10

:01

6:1

1:2

86

:12

:54

6:1

4:2

1





Callout

Open NP


Callout

Restore NP


Callout

ATS switch from Gen to NP


Callout

AHU-2 & EF-1,2 at setpoint

IST 3 - UK HSRB 4th flr BSL-3 - 2021-07-28_0447_Open NP


-0.35

-0.3

-0.25

-0.2

-0.15

-0.1

-0.05

0

0.05

0.1

4:5

8:4

0

4:5

8:4

2

4:5

8:4

3

4:5

8:4

5

4:5

8:4

6

4:5

8:4

8

4:5

8:4

9

4:5

8:5

1

4:5

8:5

2

4:5

8:5

4

4:5

8:5

5

4:5

8:5

7

4:5

8:5

8

4:5

9:0

0

4:5

9:0

1

4:5

9:0

3

4:5

9:0

4

4:5

9:0

6

4:5

9:0

7

4:5

9:0

9

4:5

9:1

0

4:5

9:1

2

4:5

9:1

3

4:5

9:1

5

4:5

9:1

6

4:5

9:1

8

4:5

9:1

9

4:5

9:2

1

4:5

9:2

2

4:5

9:2

4

4:5

9:2

5

4:5

9:2

7

4:5

9:2

8

4:5

9:3

0

4:5

9:3

1

4:5

9:3

3

4:5

9:3

4

4:5

9:3

6

4:5

9:3

7

4:5

9:3

9

4:5

9:4

0

4:5

9:4

2

4:5

9:4

3

4:5

9:4

5

4:5

9:4

6

4:5

9:4

8

4:5

9:4

9

4:5

9:5

1

4:5

9:5

2

4:5

9:5

4

4:5

9:5

5

4:5

9:5

7

4:5

9:5

8

5:0

0:0

0




IST 3 - UK HSRB 4th flr BSL-3 - 2021-07-28_0447_Restore NP


-0.35

-0.3

-0.25

-0.2

-0.15

-0.1

-0.05

0

0.05

0.1

0.15

5:3

1:0

05

:31

:05

5:3

1:1

05

:31

:15

5:3

1:2

05

:31

:25

5:3

1:3

05

:31

:35

5:3

1:4

05

:31

:45

5:3

1:5

05

:31

:55

5:3

2:0

05

:32

:05

5:3

2:1

05

:32

:15

5:3

2:2

05

:32

:25

5:3

2:3

05

:32

:35

5:3

2:4

05

:32

:45

5:3

2:5

05

:32

:55

5:3

3:0

05

:33

:05

5:3

3:1

05

:33

:15

5:3

3:2

05

:33

:25

5:3

3:3

05

:33

:35

5:3

3:4

05

:33

:45

5:3

3:5

05

:33

:55

5:3

4:0

05

:34

:05

5:3

4:1

05

:34

:15

5:3

4:2

05

:34

:25

5:3

4:3

05

:34

:35

5:3

4:4

05

:34

:45

5:3

4:5

05

:34

:55

5:3

5:0

05

:35

:05

5:3

5:1

05

:35

:15

5:3

5:2

05

:35

:25

5:3

5:3

05

:35

:35

5:3

5:4

05

:35

:45

5:3

5:5

05

:35

:55




IST 3 - UK HSRB 4th flr BSL-3 - 2021-07-28_0447_ATS sw Gen NP


-0.25

-0.2

-0.15

-0.1

-0.05

0

0.05

5:3

7:1

05

:37

:11

5:3

7:1

15

:37

:12

5:3

7:1

25

:37

:13

5:3

7:1

35

:37

:14

5:3

7:1

45

:37

:15

5:3

7:1

55

:37

:16

5:3

7:1

65

:37

:17

5:3

7:1

75

:37

:18

5:3

7:1

85

:37

:19

5:3

7:1

95

:37

:20

5:3

7:2

05

:37

:21

5:3

7:2

15

:37

:22

5:3

7:2

25

:37

:23

5:3

7:2

35

:37

:24

5:3

7:2

45

:37

:25

5:3

7:2

55

:37

:26

5:3

7:2

65

:37

:27

5:3

7:2

75

:37

:28

5:3

7:2

85

:37

:29

5:3

7:2

95

:37

:30

5:3

7:3

05

:37

:31

5:3

7:3

15

:37

:32

5:3

7:3

25

:37

:33

5:3

7:3

35

:37

:34

5:3

7:3

45

:37

:35

5:3

7:3

55

:37

:36

5:3

7:3

65

:37

:37

5:3

7:3

75

:37

:38

5:3

7:3

85

:37

:39

5:3

7:3

95

:37

:40






Start time: 4:47:51

End time: 6:15:37

DP Array Ch # 1 2 3 4 5 6 7 8 9

Room DP monitored

Corridor

400N /

Ante

Room 464

Ante

Room 464

/ Airlock

no #

Airlock no

# / Lab

464G

Lab 464G

/ Lab 464F

Lab 464G

/ Animal

Room

464H

Lab 464G

/ Animal

Room

464J

Lab 464G

/ Lab 464L

Decontam

464D /

Lab 464G

Decontam

464M /

Lab 464G

Average (in wc) -0.1524 -0.1016 -0.1238 -0.0680 -0.0568 -0.1323 0.0038 -0.1433 -0.1473

Min value (in wc) -0.2908 -0.2903 -0.2910 -0.2197 -0.1551 -0.2332 -0.2237 -0.2900 -0.2913

Max value (in wc) 0.0083 0.0077 0.0100 0.0643 -0.0025 -0.0024 0.1204 0.0099 0.0341

COUNT above 0.005 2.0 2.0 3.0 308.0 0.0 0.0 4041.0 2.0 1.0

Seconds above neutral 1 1 1.5 154 0 0 2020.5 1 0.5

Data and Graph event: Open Normal Power (NP)

Start time: 4:58:40

End time: 5:00:00

DP Array Ch # 1 2 3 4 5 6 7 8 9

Room DP monitored

Corridor

400N /

Ante

Room 464

Ante

Room 464

/ Airlock

no #

Airlock no

# / Lab

464G

Lab 464G

/ Lab 464F

Lab 464G

/ Animal

Room

464H

Lab 464G

/ Animal

Room

464J

Lab 464G

/ Lab 464L

Decontam

464D /

Lab 464G

Decontam

464M /

Lab 464G

Average (in wc) -0.1808 -0.0661 -0.0785 -0.0070 -0.0377 -0.0511 0.0175 -0.1092 -0.0761

Min value (in wc) -0.2908 -0.1998 -0.2499 -0.0595 -0.1103 -0.1363 -0.0228 -0.2900 -0.2212

Max value (in wc) -0.0032 -0.0007 0.0015 0.0006 -0.0047 -0.0024 0.0779 -0.0020 -0.0011

COUNT above 0.005 0.0 0.0 0.0 0.0 0.0 0.0 91.0 0.0 0.0

Seconds above neutral 0 0 0 0 0 0 45.5 0 0

Data and Graph event: Restore Normal Power (NP) Data and Graph event: ATS switch from Generator to NP

Start time: 5:31:00 Start time: 5:37:10

End time: 5:36:00 End time: 5:37:40

DP Array Ch # 1 2 3 4 5 6 7 8 9 DP Array Ch # 1 2 3 4 5 6 7 8 9

Room DP monitored

Corridor

400N /

Ante

Room 464

Ante

Room 464

/ Airlock

no #

Airlock no

# / Lab

464G

Lab 464G

/ Lab 464F

Lab 464G

/ Animal

Room

464H

Lab 464G

/ Animal

Room

464J

Lab 464G

/ Lab 464L

Decontam

464D /

Lab 464G

Decontam

464M /

Lab 464G

Room DP monitored

Corridor

400N /

Ante

Room 464

Ante

Room 464

/ Airlock

no #

Airlock no

# / Lab

464G

Lab 464G

/ Lab 464F

Lab 464G

/ Animal

Room

464H

Lab 464G

/ Animal

Room

464J

Lab 464G

/ Lab 464L

Decontam

464D /

Lab 464G

Deconta

m 464M /

Lab 464G

Average (in wc) -0.2778 -0.1357 -0.1651 -0.0147 -0.0423 -0.1318 -0.0086 -0.2001 -0.2044 Average (in wc) -0.1250 -0.0264 -0.0283 -0.0181 -0.0307 -0.1456 -0.1493 -0.0483 -0.1418

Min value (in wc) -0.2907 -0.2258 -0.2812 -0.1259 -0.1004 -0.1623 -0.1722 -0.2899 -0.2717 Min value (in wc) -0.2362 -0.0389 -0.0424 -0.0411 -0.0662 -0.2332 -0.2237 -0.0721 -0.2007

Max value (in wc) -0.1394 -0.0283 -0.0306 0.0643 -0.0025 -0.1142 0.0924 -0.0562 -0.1433 Max value (in wc) 0.0083 0.0077 0.0100 0.0212 -0.0115 -0.0662 -0.0738 0.0099 -0.0416

COUNT above 0.005 0.0 0.0 0.0 295.0 0.0 0.0 229.0 0.0 0.0 COUNT above 0.005 1.0 2.0 3.0 1.0 0.0 0.0 0.0 2.0 0.0

Seconds above neutral 0 0 0 147.5 0 0 114.5 0 0 Seconds above neutral 0.5 1 1.5 0.5 0 0 0 1 0



PV Report - 2021


Supporting Documents