Construction of Confocal Raman Microspectrometer and Examination

of Possible Modifications for the Detection of Bacteria in vivoShannon Harrison

Megan McInerney

Advisor: Anita Mahadevan-Jansen

Project Description

• Immediate Goals: – Construct Confocal Raman Microspectometer similar to one

designed by G.J. Puppels– Propose design modification for use on living subjects

– Create spreadsheet incorporating spectral data– Design molecular bond database

• Ultimate Desire: Construct a probe utilizing confocal Raman microspectroscopy that will accurately detect and identify bacteria present in middle ear causing infection

Motivation and Benefits

• Eliminate “troubleshooting” approach to treating middle ear infections

• Construct a confocal Raman system that can be used for in vivo analysis

• Reduce cost of health care

• Proposed probe design more sterile, less expensive and quicker than swab analysis

Raman Spectroscopy

• What

• Why– Advantages– Disadvantages

• How

System Requirements

• Short integration time

• Input spectral data

• Analyze the spectra by matching the peaks to a database of standards

• Output responsible bacteria

• Portable

Confocal Raman Microspectometer

Design Flowchart

Apparatus Modifications

• Using fiber optic cables, construct probe including the objective and bandpass filters

• Align and fix optical components to make apparatus portable

Data Analysis

• Understand the basis of Raman Spectroscopy• Compile a list of common spectral peaks• Create a temporary spreadsheet containing the bond and Raman

shift• Become familiar with Matlab• Design a program that will input sample spectra and identify the

bonds by matching peaks• Use the probe to determine spectra for bacteria causing Otitis

Media • Integrate the above spectra into the database program• Test the program for recognition of the bacterial spectra

Database Flow Chart

Example Spectrum

Raman Spectroscopic Information

Safety Precautions

• Environment

• Thermal tissue damage– standard regulation

• Maintaining sterile techniques

• Accuracy

Preliminary Conclusions

• Construction, alignment, and preliminary testing feasible by end of semester

• Probe designed by end of semester

• We suggest that this project is continued next semester by BME 272/273 students to complete construction and testing of probe as well as implement molecular bond database

References

• Http://www.badertech.com

• Http://www.apptechsys.com

• Customer service personnel from:

– MSDSpro

– Nousoft

– Ovid

• Pedro Pedroso

• Anita Mahadevan-Jansen

• Introductory Raman Spectroscopy, Ferraro Makamato

• Raman Spectroscopy, Long, D.A.

• Raman Spectroscopy for the Detection of Cancers and Precancers, Mahadevan-Jansen, Antia

• Confocal Raman Spectroscopy, Puppels, G.J.