6/22/2015 Conjugated linoleic acid (CLA) inhibits growth of Caco­2 colon cancer cells: possible mediation by oleamide. ­ PubMed ­ NCBI

http://www.ncbi.nlm.nih.gov/pubmed/12174903 1/2

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Anticancer Res. 2002 Jul­Aug;22(4):2193­7.

Conjugated linoleic acid (CLA) inhibits growth of Caco­2 colon cancer cells:possible mediation by oleamide.Kim EJ , Jun JG, Park HS, Kim SM, Ha YL, Park JH.

AbstractWe have previously observed that dietary conjugated linoleic acid (CLA) inhibited colontumorigenesis induced by 1,2­dimethylhydrazine in rats. The present study was performed todetermine the mechanisms by which CLA inhibits colon cancer cell growth. CLA markedly inhibitedCaco­2 cell growth, while linoleic acid (LA) slightly increased growth. Both CLA and LA increased theproduction of material reactive to antibodies against prostaglandin (PG)E2 and leukotriene (LT)B4,estimated by a competitive enzyme immunoassays (EIA), in a dose­dependent manner. However,the magnitude of the increase was markedly higher with CLA than that with LA, suggesting that thismaterial was not PGE2 or LTB4. The active compound was isolated by thin­layer chromatographyand the nuclear magnetic resonance and infrared spectra revealed that the structure was identicalto that of oleamide. The purified oleamide inhibited cell growth and cross­reacted with the EIA.These results indicate that inhibition of Caco­2 cell growth by CLA may be due in part to increasedoleamide production.

PMID: 12174903 [PubMed ­ indexed for MEDLINE]

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6/22/2015 Conjugated linoleic acid (CLA) inhibits growth of Caco­2 colon cancer cells: possible mediation by oleamide. ­ PubMed ­ NCBI

http://www.ncbi.nlm.nih.gov/pubmed/12174903 2/2