Paul Mackin, Lisa Young, Steve KupczakCambridge Institute, Robinson Way, Cambridge CB2 0RE.

Tumour Models Core - Biological Resource Unit

A wealth of experience working with

pancreatic and prostate cancer models

and study support

Comprehensive technical assistance

with studies

Therapeutic study management

Short term and long term survival study

management

Drug dosing/scheduling

Study database management

Surgical intervention

Dedicated drug preparation, surgical &

necropsy areas

Tissue and blood collection

Necropsy

Tissue fixation

The BRU/Tumour Models Core continues to provides researchers with sound advice, expert skills and technical assistance enabling their continued and on-going research. These model are likely to be more predictive for novel treatments which can be translated to patients in clinical practice.We will endeavour to explore the possibilities of refining the models and expanding our services. For details and information please contact: Lisa.young@cruk.cam.ac.uk Paul.mackin@cruk.cam.ac.uk

KPC mice are generated and, from 2 months of age, are abdominally palpated weekly to detect the early development of a pancreas tumour (protocol 2).

Mice with a palpable tumour are ultrasound

imaged to confirm the presence and follow the growth

of the tumour (protocol 3).

Mouse with pancreas tumour detected by

palpation &/or ultrasound imaging is transferred to another

project licence or recognised scientific

establishment for experiments intended to

inform the field of pancreas cancer

research.

Mouse has a tumour too

small for enrolment onto

study.Mouse has a tumour size,

location and type suitable for

study.

Mouse has an unsuitable tumour

and/or shows signs of ill health

and pain.

Mouse is enrolled onto a study, primarily

therapeutics but also basic biology and diagnostics (PPL

transfer).

Mouse is killed and, if appropriate, tissue

collected for in vitro studies (therapeutics,

basic biology and diagnostics).

Refining and reducing the stress of mice being repeatedly imaged to follow the growth pattern of the tumours as well as the associated implications of repeated exposure to anaesthetic.

Refinement - preparation of mice - quality of images - speed of requisition - reproducibility - accuracy

Reduction - induction - anaesthesia - i.p saline 2mls - no i.p saline for study mice - recovery

A new initiative recently developed within the Biological Resources Unit at The Cambridge Institute. Co-managed by the Production and Study Managers with a dedicated Senior animal technician (NACWO).

Researchers joining the Institute are able to ‘hit the ground running’ as we provide full study support and the central supply of experimental and control mice.

Requests for services and supply are received from researchers at the CI as well a affiliates to CRUK and commercial collaborators, these are discussed and prioritised at the Core Steering Group which meet every two months.

The core has initiated an approach in anticipating user requirements, processing requests and cross-charging for services and supplies rendered.

The TMC have the experience, principles and flexibility to adapt to other tumour models - Breast, Prostate, Liver and Kidney cancers.

Paul MackinProduction Manager

Lisa YoungStudy

Manager

Steve KupczakNACWO

Micro bubbles

IVIS Spectrum in-vivo imaging

Split screen

Pancreas

Embryos

Prostate

Kidney

Vevo 2100 high resolution in-vivo imaging

The Tumour Models Core