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Conference on:

BIOLOGICAL AND CLINICAL ASPECTS OF THE USE OF SOMATIC STEM CELLS IN REGENERATIVE MEDICINE

December 16th-17th, 2013

Wroclaw, Poland

under the auspices of

Rafał Dutkiewicz Aleksander Marek Skorupa

The President of Wroclaw The Governor of Lower Silesia

The Polish Academy of Sciences

The Polish Society of Regenerative Medicine

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organized by

L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences (Wroclaw)

Lower Silesian Center for Cellular Transplantation and National Bone Marrow Donor Registry (Wroclaw)

Association for the Benefit of Development of Donation of Marrow (Wroclaw)

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CONTENTS

TABLE OF CONTENTS

WELCOME TO WROCLAW 4

PREVIOUS INTERNATIONAL CONFERENCES 6

WROCLAW – the host city 9

ESTHETIC SUPPORT 11

ORGANIZING COMMITTEE 20

GENERAL INFORMATION 21

CONFERENCE PROGRAMME 26

ORAL AND POSTER CONTRIBUTION 33

ACKNOWLEDGEMENTS 41

LIST OF INVITED SPEAKERS AND CHAIRPERSONS

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PARTICIPANTS 43

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WELCOME TO WROCLAW It is my great pleasure to extend to you my sincerest invitation to participate in the conference on “BIOLOGICAL AND CLINICAL ASPECTS OF THE USE OF SOMATIC STEM CELLS IN REGENERATIVE MEDICINE” taking place in Wroclaw on December 16th-17th, 2013. The progress in this field and a great array of exciting possibilities to employ stem cells in the clinic make many of us interested to participate actively in stem cell research and in many aspects of their clinical translation. For all of this we are organizing the conference. It is our intention to focus on marrow derived stem cells and in particular on (I) description of their developmental hierarchy, (II) regulation of their mobilization and homing and finally (III) the potential mechanisms how they may contribute to regeneration of damaged tissues. The marrow is rich in various progenitors including VSELs, mesenchymal/stromal, endothelial stem cells and the probably best known hematopoietic stem cells. We will be happy to host participants involved in genetic, phenotypic and functional description of stem cells at different levels of commitment to some lineages. Translation of the research to the clinic is very welcome for both physicians and researchers. Therefore we will organize a session and a workshop on the methods used for enrichment of the marrow cell population in stem cells to use them in the clinic directly after isolation or after ex vivo expansion. We are organizing practical workshops on that, including live ongoing internet-based presentation of ex vivo propagation of stromal stem cells. An important part of the conference will consist of clinical reports of the use of some progenitor cells in wound healing, revascularization, and rebuilding of damaged cartilage and bones. In spite of some encouraging data in this area our understanding of the mechanisms involved in these processes

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is rather poor. Still we are discussing whether paracrine activity of the implanted cells or their own propensity to expand in vivo plays a key role. The key issue of the translation of stem cell research to their clinical application is the safety of their use. Thus, we will be happy as organizers to have during the conference a panel discussion on practical implementation of the EU directives in the field of regulation of advanced therapy medicinal products in Europe.

I do hope that your active participation in the conference will be relevant and your studies are contributing significantly to the literature with a final outcome as in the verse:

With a finger wrapped in brown paper I am trying to link the past and the future With blue flame And white smoke rises up into rough clouds (Magdalena Lange)

I heartily welcome all of you to Wroclaw.

The winter holiday is just around the corner. Let us enjoy the most charming and festive atmosphere of Christmas time. Happy New Year 2014.

Andrzej Lange

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PREVIOUS INTERNATIONAL CONFERENCES 1. “Epidemiological, Immunological and Genetical Aspects of Asbestosis”, 24-26.03.1981 organized by: Institute of Immunology and Experimental Therapy of The Polish Academy of Sciences Proceeding published: Archiwum Immunologiae et Therapiae Experimentalis, Epidemiological Immunological and Genetical Aspects of Asbestosis, Vol 30, No 3-4, 1982 2. “Molecular biology techniques in clinical immunology and transplantation”, 15-18.12.1993, Wroclaw organized by: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy Commission of Immunotherapy of the Polish Academy of Sciences, Polish Society of Immunology Proceeding published: Abstracts book - Molecular biology techniques in clinical immunology and transplantation, 1993 3. 3rd Central European Transplant Conference “The present and perspectives of immunogenetics and transplantation immunology and clinical aspects of blood/bone marrow transplantation”, 15-17.12.1997, Wroclaw organized by: BMT Unit, K. Dłuski Hospital, Insitute of Immunology and Experimental Therapy in 10th Anniversary of its activity Proceeding published: Bone Marrow Transplantation, Vol. 22, Supplement 4, December 1998

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4. “Standardization of Donor-recipient matching in transplantation: the presence and perspectives in Central-East European Associated States”, 28-29.04.2003, Warsaw organized by: Lower Silesian Center for Cellular Transplantation with National Polish Bone Marrow Donors Registry on its 15th, Anniversary of transplant activity on behalf of the Polish Society for Immunogenetics, the Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences, Wroclaw, the Institute of Hematology and Blood Transfusion, Warsaw and the STEMNET Consortium Proceeding published: Standardization of Donor-Recipient Matching in Transplantation, Nova Science Publishers, 2006 5. “Immunogenetics in haematology and stem cell transplantation”, 8–10.02.2006, Wroclaw organized by: Polish Society for Immunogenetics, Association for the Benefit of Development of Marrow Donation, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Lower Silesian Centre for Cellular Transplantation with the National Polish Bone Marrow Donor Registry, Wroclaw, Commission of Transplantology, Polish Academy of Sciences Proceeding published: International Journal of Immunogenetics, Vol. 35, No 4/5, August/October 2008 6. “Donor Recipient Matching and Cellular Therapy in Transplantation: technological and clinical aspects”, 23–24.04.2009, Wroclaw organized by: Polish Society for Immunogenetics, Association for the Benefit of Development of Marrow Donation, L. Hirszfeld Institute of Immunology and

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Experimental Therapy, Polish Academy of Sciences, Lower Silesian Centre for Cellular Transplantation with the National Polish Bone Marrow Donor Registry Proceeding published: Transplantation Proceedings, Vol. 42, No 8, October 2010 7. “Practical Aspects of Donor-Recipient Matching in HSCT”, 17-18.12.2012, Wroclaw organized by: L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Lower Silesian Center for Cellular Transplantation and National Bone Marrow Donor Registry, Association for the Benefit of Development of Donation of Marrow, Wroclaw As a continuation of the Special Issue titled "Biological and Genetic Aspects of Donor-Recipient Matching in HSCT" published in "Bone Marrow Research", volume 2012 Organization of scientific symposia serving as a platform for discussion and spreading information is the legacy of our activity. But to avoid our unrealistic ambition it is good to remember my daughter Magdalena’s verse inspired by a Polish proverb: even having an open window you are not flying, as opposed to the Fly Eagle Fly: Window, I am opening, Leaving open and Falling down

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WROCLAW

Wroclaw is situated at a crossroad whose branches link the town with three European capitals: Warsaw, Prague and Berlin. The crossroads location is reflected in the city’s varied history. Founded in the 10th century, Wroclaw has a rich and unique history, reflecting the Polish, Czech and Prussian-German spirit. This crossroads location is reflected by the colour of the town, which you can see looking at buildings, parks and gardens.

This island of churches recalls the time of the Piast Dynasty. The Arch-Cathedral of St. John the Baptist is visible from far

away and it is undoubtedly Wroclaw's most precious monument of sacred architecture (note that it is situated in the vicinity of the JP II Hotel). The church has over 1000 years of history, beginning soon after the Emperor Otto III and the Pope Silvestre II established a diocese in Wroclaw. Such an honour was a proof of the city's great importance, as a centre of politics,

economy and culture in the region. A number of turbulent events swept across the town, especially during the Thirty Years' War. Prussia and its successor Germany brought new changes. Wroclaw, under the name Breslau, was the second town of Germany after Berlin and flourished in the fields of art and science. Numerous Nobel Prize winners had their roots in Lower Silesia and they all worked in Wroclaw. Important names strongly associated with the city, like Naisser, Koch, Rokitansky and Hirszfeld, have coloured the map of medical scientific progress.

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The German-Polish sense of art and music, as well as perfect and beautiful urbanization of the city, is a heritage by itself, among which classicistic (note the Wroclaw Opera House, which you may visit to enjoy the performance) and modernistic architecture buildings are most worth seeing (please join the City Tour). Not to be missed is the University

with a baroque Leopoldine Saal, facing the Odra River bank. The Odra is a river of over a hundred bridges. They witness the progress in engineering and technical skill. You can walk over the Sand Bridge, built in 1861, or drive via new monumental bridges.

The strengths of Wroclaw rely on the multicultural roots, where German perfectionists merged with the Slavic soul, resulting in the beautiful town bridging the nations which proudly becomes the European Capital of Culture in 2016.

Magdalena Lange

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ESTHETIC SUPPORT TO THE CONFERENCE WAS LEND BY: BRONISŁAW KRZYSZTOF, DARIA ILOW, MAGDALENA LANGE,

ADAM CZERMAK, NATALIA HALICKA, MONIKA PAWLAS-KRUK, CHAMBER CHOIR 'SENZA RIGORE'

BRONISŁAW KRZYSZTOF

“Bronislaw Krzysztof knows how to translate emotion and energy into the visual with technical proficiency. He has the capability of translating invisible to visible. His surrealistic expression achieved with masterly classical technique makes Bronisław Krzysztof one of the most unique contemporary sculptors." Mashiko New York

"The urge to touch may not be merely a response to perfection of outward form, but , sometimes also a reaction to the attractive power of the life within a person or a created object. The artist strives to produce the illusion of that inner life in his creations. This, I feel, is one of the most important elements in Krzysztof's work. Other contemporary sculptors, including Anthony Gormley and David Mach, also deal with the human figure in their different ways, but they rarely penetrate so far below the surface." Huon Mallalieu British Museum, London

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DARIA ILOW

Daria Ilow was born on 1979 in Wroclaw, Poland. In 2001 she graduated from the "Pho-Bos" Photography College in Wroclaw and in 2005 from the Academy of the Fine Arts in Gdańsk. She belongs to the Association of Polish Art Photographers. Her photography is on the border of painting and graffiti. Her photos are a collection of various issues, ideas, things and unfinished conversations. They include happiness and pain, joy and sadness. Photography for Daria is an escape into another reality, another world of the form, character and shape. Individual and collective exhibitions: 2011 - collective exhibition of the Association of Polish Art Photographers at the Lower Silesian Photography Centre "Domek Romański" in Wroclaw 2010 - individual photography exhibition at Association of Polish Art Photographers Gallery in Wroclaw 2009 - collective exhibition of the Association of Polish Art Photographers at the Lower Silesian Photography Centre "Domek Romański" in Wroclaw 2008 - individual exhibition "Fotografiti" at the Lower Silesian Photography Centre "Domek Romański" in Wroclaw, 2005 - collective exhibition "LUX" at the Lower Silesian Photography Centre "Domek Romański" in Wroclaw, 2004 - individual exhibition "Transfiguracje" at Contemporary Art Gallery in Wroclaw within POLFOTO International Festival of Photography 2001 - diploma work at the "Pho-Bos" Photography College, collective exhibition at Design Gallery in Wroclaw

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MAGDALENA LANGE Magdalena Lange is a PhD student conducting research on the bio-art in the Philosophy Departament at the Jagiellonian Univesrsity in Krakow. She is graduated biotechnologist and art historian. She studied medicine, literature and photography. She used to write poems, which were published in the annals of literature. Recently she published a separate volume by the

Kracow Publishing Company.

ADAM CZERMAK

Adam Czermak (born 21.07.1983) graduated with distinction from the Henryk Wieniawski Secondary Music School in Poznań (2001) and the Ignacy Paderewski Academy of Music in Poznan in the violin class of Prof. Bartosz Bryła. He continued his studies in Austria (Universitaet fuer Musik und darstellende Kunst Graz) with Prof. Yair Klessa and Silvii Marcovici. In 2005 he was awarded a 2-year scholarship of the Opera in Zurich

where he studied with Bartłomieja Nizioła and Hanny Weinmeister. Moreover Adam Czermak took part in many International Master Classes in Poland, Austria, Germany and Spain where he perfected his skills with such great Professors like Jadwiga Kaliszewska, Mirosław Lawrynowicz, Jewgenij Bushkow, Eduard Grach, Rene Staar or Grigorij Zyslin. Adam Czermak is the laureate of main awards in many national and international competitions like the Stanislaw

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Hajzer Bach Competition in Zielona Góra, the Stanislaw Serwaczynski Competition in Lublin, the International Competition for Young Musicians in Belgium, the International Violin Competition in Schloss Zell an der Pram in Austria. In 2005 he won one award and one gold medal at the European Competition for Young Soloists in Luxembourg. The artist was the concertmaster of the International Opera Studio in Zurich (Switzerland), Oviedo Filarmonia in Spain and Goettinger Symphonie Orchester in Germany. Since 2010 he has been a concertmaster of Wroclaw Opera. In his career he has cooperated with the most outstanding conductors like Zubin Mehta, Nello Santi, Franz Welser-Moest, Friedrich Haider, Tadeusz Strugała and Jacek Kaspszyk.

NATALIA HALICKA

Natalia Halicka is a student of the second year of master studies at the Vocal Faculty of the Karol Lipinski Academy of Music in Wroclaw in prof. Ewa Czermak’s class. She finished master studies at the Faculty of Music Education in the conducting class of prof. Bogusława Orzechowska. She took part in many interpretation competitions in Wroclaw, Duszniki-Zdrój, Krynica-Zdrój and Radziejowice conducted by prof. Teresa Żylis-Gara, prof. Ingrid Kremling, prof. Stefania Kałużanka, prof. Eugeniusz Sąsiadek, prof. Helena Łazarska, prof. Ella Susmanek. She is a perfomer of many chamber concerts and vocal recitals. Her achievements comprise: - honorable mention at XIth International Stanislaw Moniuszko Competition, Evening Song (Białystok 2011) - 1st Reward at IIIrd International Rudolf Petrak Vocal Competition (Żylina, 2012)

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- finalist of International Vocal Competition (Żdar nad Sazavou, 2012) - finalist of Xth Interschool Competition of Polish Artistic Song by Edmund Kossowski (Warszawa 2012) - 1st Reward of VIth International Halina Halska-Fijalkowska Competition - Special Reward for the best participant of the competition coming from Lower Silesia and Opole Voivodship in the form of a 4-day-stay in the European Parliament (2013) - Reward of the Marshall of the Lower Silesia Voivodship for outstanding achievements for culture (2013) - scholarship holder of the Minister of Science and Higher Education for outstanding achievements in the school year 2012/2013 - scholarship holder of the President of Wroclaw in art and culture for young and skilled Wroclaw inhabitants (2013)

JOLANTA SZYBALSKA-MATCZAK

She graduated from the Faculty of Music Education at the Karol Lipinski Academy of Music in Wroclaw and continued her education at Postgraduate Choirmaster Studies in Bydgoszcz. Since 1991 she has been working at her parent Academy and training young people the art of conducting. She is an artistic director of Choir 'Feichtinum' and Chamber Choir 'Senza Rigore' which accompanies her in her local and foreign voyages taking part in many musical events and choir competitions where she wins many prestigious awards and honorable mentions. At present she is working at the position of the professor of the Academy of Music. In the years 2008-2012 she was a deputy dean and

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since September 2012 she has been nominated for the position of dean of the Faculty of Music Education, Choral and Church Music at the Karol Lipinski Academy of Music in Wroclaw. She cooperates with the Polish program of school chorus development “Singing Poland” and is the lecturer at the Polish Choral Conducting School in Koszalin. Moreover she takes part in various choral competitions as a jury member. She is a laureate of honorable mention for the best conductor at the XXth Polish Festival of Sea Song in Wejherowo, rector distinctions like the Wroclaw Academy of Music Rector Reward, silver distinction “Oder of Merit for the City of Rybnik”, honorable distinction “Order of Merit for the Polish Culture” and the Bronze Cross of Merit.

CHAMBER CHOIR 'SENZA RIGORE' The Chamber Choir ‘Senza Rigore’ of the Academy of Music in Wroclaw was formed in October 2000. The ensemble gathers the Academy’s students and graduates who are particularly keen on singing in a choir. Its name, proposed by one of its members, is to emphasize the voluntary nature of its membership, and above all the fact that it operates outside the obligatory curriculum. Its founder, art director and choirmaster is dr hab. Jolanta Szybalska-Matczak, a graduate from the Karol Lipinski Academy of Music in Wroclaw, who studied under Prof. Zofia Urbanyi-Krasnodębska and also completed the Post-Graduate Choirmaster Programme of Study at Bydgoszcz.

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The intensive work of the choir members, who share a common passion, quickly led to a debut at the 27th Miner’s Day Festival of Academy Choirs in Wroclaw in December 2000. Since then the Chamber Choir ‘Senza Rigore’ has represented the Academy of Music in Wroclaw at many prestigious national and international concerts and festivals, including: The International Organ Music Festival ‘Cantus Organi’ at Wołów, The Wieczory Tumskie (Evenings in Cathedral Island) Festival in Wroclaw, The Choir Meetings in Gliwice, The Chamber Music Festival ‘Ave Crux’ at Czernica Wroclawska, The 15th May with Old Music Festival in Wroclaw, The 2006 Transnaturale Festival at Schloss Uhyst in Germany, The 21st Organ Conversatorium in Legnica, The International Choir Festival in Hradec Králové and The 3rd International Choir Conducting Tournament ‘Towards Polyphony’ at the Academy of Music in Wroclaw. The choir has also performed at cyclical concerts held at the Academies of Music in Warsaw (‘Interpretations of Old Music’), Bydgoszcz (‘Music on Tuesday’) and Wroclaw (‘The Wroclaw School of Choirmasters’), as well as in Ostrava and in Dresden’s Cathedral.

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In 2004 the choir took part in the making of a recording to celebrate the 125th anniversary of granting the title ofdoctor honoris causa of Wroclaw University to Johannes Brahms, performing, together with organist Piotr Rojek, the composer’s Psalm 23 op. 27 for Choir and Organ. The Chamber Choir ‘Senza Rigore’ has also participated in various competitions, winning numerous awards and distinctions (the last time the Gold Medal at the Musica Religiosa Festival in Olomouc in 2009). The long list of its trophies attests to the commitment and excellent vocal skills of its members. Each of them, despite their tight schedules, devotes a lot of effort and time to the choir. ‘Senza Rigore’ is a place for enthusiasts, who pursue in it their passion for collective singing. The fact that the passion translates into competition successes, which brings credit to the Academy, is a source of genuine satisfaction to the choir members and its conductress.

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Fotografiti Daria Ilow

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ORGANIZING COMMITTEE Andrzej Lange, president Mariusz Ratajczak Barbara Łukomska Ewa Zuba-Surma Emilia Jaskuła Sylwia Mizia Alicja Hetnar

CONFERENCE OFFICE

Tomasz Borodzicz Jakub Kwiatkowski

Patrycja Bratos Barbara Szymczak

Agata Borowik

Dorota Dera-Joachimiak

Mieczysław Domagalski

Aleksandra Erbert

Justyna Godyń

Halina Kosatka

Lower Silesian Center for Cellular Transplantation and National Bone Marrow Donor Registry

105, Grabiszynska Street 53-439 Wroclaw, Poland tel. +48717831375 fax: +48713621512

e-mail: conference2013@dctk.wroc.pl conference website: www.conference2013.dctk.wroc.pl

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GENERAL INFORMATION

Conference Venue:

December 16th

Plenary and Poster Sessions

John Paul II Hotel

2, Sw. Idziego,

50-328 Wroclaw

December 17th

Plenary and Poster Sessions

John Paul II Hotel

Workshops

Scientific-Medical Incubator of the Lower Silesian Centre for Cellular Transplantation

18, Muchoborska Street,

54-424 Wroclaw

Buildings:

Beta, ground floor - seminar room

Gamma, ground floor - laboratories

Telephone/fax during the conference: +48717911990,

mobile: +48601581550

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Shuttle bus (December 17th, 1 am) will be provided to take you from the John Paul II Hotel to the Scientific-Medical Incubator of the Lower Silesian Centre for Cellular Transplantation.

The official language of the conference is English.

The conference is sponsored by the Polish Academy of Sciences. Lunch, coffee and tea

During the conference session breaks coffee and lunch will be served free of charge to delegates wearing their name badge. Public Transport

Starting Point Destination Means of Transport

John Paul II Hotel

Dominikanski Square Railway Station

Tram line: 9, 17

Market Square Town Hall

Tram line: 10

Wroclaw University of

Environmental and Life Science Students Hotel

John Paul II Hotel Tram line: 2, 10

Taxi

If you take a taxi labelled Royal Taxi, a 10% discount will be offered. To be serviced please call: +48717072810, +48530303020.

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WROCLAW CITY MAP

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INFORMATION FOR SPEAKERS AND POSTER PRESENTATION Audiovisual Equipment The lecture room will be equipped with a computer for PowerPoint presentations. PowerPoint presentations must be handed in via CD-ROM, USB compatible memory stick not later than one hour before the beginning of the session. Poster Presentations Posters will be displayed in the Caritas Room on the underground floor. All accepted posters should be 100 cm height and 80 cm width. Posters should be installed on the numbered boards and should display: the title, authors and institutions of origin. Conference staff will provide technical means to install posters.

CONTINUING MEDICAL EDUCATION (CME)

Participants are granted with 14 points of the Continuing Medical Education (CME) system. A certificate of attendance

will be available.

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Fotografiti Daria Ilow

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CONFERENCE PROGRAMME

Monday, December 16th, 2013 John Paul II Hotel

06:30-08:00 Registration at the John Paul II Hotel 2, Sw. Idziego, 50-328 Wroclaw, Poland

08:00-08:05 Andrzej Lange Welcome

08:05-08:30

Andrzej Górski Vice President of the Polish Academy of Sciences - Welcome and lecture: European Group on Ethics in Science and New Technologies (EGE) and its role in promotion of ethical standards in European science

SESSION 1: Biology of stem cells – hierarchy and diversity

of marrow derived cells

Chairpersons Andrzej Górski (Warszawa) Mariusz Ratajczak (Louisville, Szczecin)

08:30-09:05 Mariusz Ratajczak (Louisville, Szczecin): Today and future of regenerative medicine

09:05-09:40

Tsvee Lapidot (Israel): Regulation of normal and leukemic human stem cells: dynamic stem cell interactions with the bone marrow microenvironment

09:40-10:05

Oral communications: Opiela J., Samiec M., Lipiński D., Romanek J. (Balice, Poznan): The increased pluripotency of cloned pig embryos reconstructed with cell nuclei of adult bone marrow-derived mesenchymal stem cells undergoing trichostatin A-dependent epigenomic transformation Zyuz'kov G.N., Suslov N.I., Danilets M.G., Zhdanov V.V., Udut E.V., Miroshnichenko L.A., Minakova M.Y., Dygai A.M. (Tomsk): The development of pharmacological strategy of cell therapy and perspectives for the creation of highly selective drugs for regenerative medicine on the basis of alkaloids

10:05-10:20 Coffee break / Poster viewing & Real time presentation of mesenchymal stromal cells expansion

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SESSION 2: VSELs – their potential and clinical applicability

Chairpersons Diane S. Krause (Yale) Ewa Zuba-Surma (Krakow)

10:20-10:55 Diane S. Krause (Yale): Engraftment of nonhematopoietic bone marrow as lung epithelial cells

10:55-11:30 Magdalena Kucia (Louisville): VSELs and their potential role in aging

11:30-12:05 Kevin Murray (New York): Better Manufacturing Practice for Cell Therapies

12:05-12:45 Lunch

SESSION 3: Lineage committed stem cells – phenotypic description and

functional potential

Chairpersons Nathalie Chevallier (Créteil) Aleksandra Klimczak (Wroclaw)

12:45-13:05 Aleksandra Klimczak (Wroclaw): Tissue distribution and phenotype characteristics of stem cells harvested from human organs

13:05-13:40 Ewa Zuba-Surma (Krakow): Identification of rare stem cell fractions in adult bone marrow

13:40-14:15 Nathalie Chevallier (Créteil): MSC and bone tissue repair

14:15-14:50 Erdal Karaoz (Kocaeli): Immunoregulatory properties of mesenchymal stem cells

14:50-15:05 Magdalena Lange (Wroclaw): Stem cells and BioArt

15:05-15:20 Coffee break / Poster viewing & Real time presentation of mesenchymal stromal cells expansion

SESSION 4: Clinical aspects of the use of advanced medical products in the

field of cellular therapy

Chairpersons Torsten Tonn (Dresden) Wojciech Witkiewicz (Wroclaw)

15:20-15:50 Andrzej Lange (Wroclaw): Revascularization potential of marrow derived mononuclear cells

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15:50-16:25

Torsten Tonn* and Andreas Zeiher (Dresden): The future of cell therapy in acute myocardial infarction *presenting

16:25-17:00 Wojciech Wojakowski (Katowice): Stem cells in cardiac regeneration

Monday, December 16th, 2013 Wroclaw Town Hall

Official Welcome and Concert**

18:00-19:30 Welcome address by Andrzej Lange Concert

** Supported by the Halina Halska-Fijałkowska Society of Vocal Music Aficionados

CONCERT

Program

B. Bartok - Romanian Dances H. Wieniawski - "Chanson Polonaise" M. de Falla - Spanish Dance F. Kreisler - "Caprice Viennois" P. Tchaikovsky - Russian Dance from "Swan Lake" Ballet

Adam Czermak - violin

Ch. Gounod „Faust” Aria of Margarita „Ah, je ris de me voir si belle” F. Maklakiewicz - "I love you" S. Rachmaninov -"Nje poi krasavica" ("Do not sing, my beauty, to me") G. Puccini - "Gianni Scichci" - Aria of Lauretta, "O mio babbino caro"

Natalia Halicka – soprano, Monika Pawlas-Kruk – piano

W.A. Mozart - "Il re pastore" - aria for soprano and violin "L'amero, saro costante"

Natalia Halicka - soprano, Adam Czermak – violin, Monika Pawlas-Kruk – piano

M. Sawa - Laudate Dominum - Alleluja R. Di Marino - Beata viscera Mariae Virginis P. D. Peretti - O sacrum convivium M. Lanaro - Haec dies C. Hanney - Christmas Angel Polish Carols: M. Kubacki - Oj, Maluśki St. Kusztyb, St. Moryto - Zaśpiewajmy Jezuskowi Pastorałka - Dnia jednego o północy J. Glenc - Do szopy, hej pasterze

CHAMBER CHOIR 'SENZA RIGORE' (Jolanta Szybalska-Matczak, Beata Krzenciessa – conducting)

Monday, December 16th, 2013 Piwnica Świdnicka

20:00-22:30 Gala Dinner

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Tuesday, December 17th, 2013 John Paul II Hotel

SESSION 5: Legal aspects of the use of advanced medical products in cellular

therapy

Chairpersons Roman Danielewicz (Warszawa) Julian R. De Havilland (Newcastle)

08:00-08:35

Torsten Tonn (Dresden): Regulation of cell based medicines in the EU -Prerequisites for manufacture and quality control of cell based medicines from the perspective of an academic center

08:35-09:10 Julian R. De Havilland (Newcastle): Management of GMP Cell Therapy Manufacture

SESSION 6: Oral communications

09:10-10:00

Oral communications: Rudakovskaya E.B., Bagatka S.S., Yurkevich M.Yu., Zafranskaya M.M. (Minsk): In vitro IL23R expression on T lymphocytes in patients with multiple sclerosis in cocultivation with mesenchymal stem cells Goltsev A.N., Rassokha I.V., Dubrava T.G., Ostankova L.V., Ostankov M.V. (Kharkov): Optimization of treatment methods for graft-versus-host disease with mesenchymal stem cells cultured on nanocoatings Gala K., Burdzińska A., Idziak M., Pączek L. (Warsaw): Syngeneic transplantation of mesenchymal stem cells induces acute inflammatory response Nowacki M., Nazarewski Ł., Judziłł A., Tyloch D., Kloskowski T., Pietkun K., Bodnar M., Marszałek A., Gagat M.,Grzanka A., Pokrywczyńska M., Krawczyk A., Drewa T. (Bydgoszcz, Warsaw): Potential clinical aspects of the use of mesynchymal stem cells intraportal injection for minimization of the ischemia reperfusion injury in a rat model - preliminary study

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10:00-10:20 Coffee break / Poster viewing & Real time presentation of mesenchymal stromal cells expansion

10:20-12:00

Oral communications: Mierzejewska K., Suszyńska E., Borkowska S., Suszyńska M., Maj M., Ratajczak J., Kucia M., Ratajczak M.Z. (Szczecin, Louisville): Novel in vivo evidence that not only androgens but also pituitary gonadotropins and prolactin directly stimulate murine bone marrow stem cells – implications for potential treatment strategies in aplastic anemias Miklikova M., Liska V.,Holubova M., Lysak D., Mirka H., Kralickova M., Vistejnova L. (Prague, Pilsen): Porcine mesenchymal stem cell staining by Fe nanoparticles as a preparation for visualising in liver regeneration Łabędź-Masłowska A., Karnas E., Berdecka D., Brzozowski T., Ratajczak M.Z., Madeja Z., Zuba-Surma E.K. (Krakow, Louisville): Activation of bone marrow-derived very small embryonic-like stem cells by acute tissue injury in vivo Duruksu G., Kabatas S., Okcu A., Civelek E., Subasi C., Turac G., Yilmaz I., Karaoz E. (Kocaeli): Recovery of motility in rats with spinal cord injury after treatment with VEGF+ mesenchymal stem cells Abrafikova L.G. (Kharkov): Application of cryopreserved embryonic fibroblasts on carriers for treatment of aseptic dermal inflammation in rats Borysov P., Dimitrov A., Goltsev A. (Kharkov): The influence of cryopreservation on the expression level of stemness-genes in mouse fetal liver cells

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Kantor A., Paprocka M., Bielawska-Pohl A., Krawczenko A., Grillon C., Kieda C., Duś D. (Wroclaw, Orleans): New insight into endothelial progenitor cells paracrine, proangiogenic properties Petrushko M.P., Pinyaev V.I., Podufaliy V.V., Pravdina S.S. (Kharkiv, Lviv): Intrauterine administration of autologous peripheral blood mononuclear cells (PBMC) increases pregnancy rate in IVF cycles with transfer of cryopreserved embryos

12:00-13:00 Lunch

13:00

Transfer (bus provided) to the Center of Research and Education of the Lower Silesian Center for Cellular Transplantation (Muchoborska Street)

WORKSHOPS

13:30-16:00

Workshops on:

The use of cytometry in identification stem cells (SCs), their functional evaluation and finally isolation by sorting, led by Ewa Zuba-Surma (Krakow) supported by Becton Dickinson

Demonstration:

Information on the availability of the laboratory equipment for molecular work, sponsored by Syngen Biotech

Molecular analysis of marrow stem cells, led by Emilia Jaskula

16:30 Coffee Buffet

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ORAL AND POSTER CONTRIBUTION*

Title Author/s Presentation

THE INCREASED PLURIPOTENCY OF CLONED PIG EMBRYOS RECONSTRUCTED WITH CELL NUCLEI OF ADULT BONE MARROW-DERIVED MESENCHYMAL STEM CELLS UNDERGOING TRICHOSTATIN A-DEPENDENT EPIGENOMIC TRANSFORMATION

Opiela J., Samiec M., Lipiński D., Romanek J.

Oral, Poster P1

THE DEVELOPMENT OF PHARMACOLOGICAL STRATEGY OF CELL THERAPY AND PERSPECTIVES FOR THE CREATION OF HIGHLY SELECTIVE DRUGS FOR REGENERATIVE MEDICINE ON THE BASIS OF ALKALOIDS

Zyuz'kov G.N., Suslov N.I., Danilets M.G., Zhdanov V.V., Udut E.V., Miroshnichenko L.A., Minakova M.Y., Dygai A.M.

Oral, Poster P2

INFLUENCE OF PHYSICAL ACTIVITY ON CIRCUALTING IN PERIPHERAL BLOOD STEM CELLS INCLUDING VERY SMALL EMBRYONIC LIKES STEM CELLS (VSELs)

Staniszewska M., Pudło A., Tarnowski M., Pędziwiatr D., Ryterska K., Wysokiński P., Stachowska E., Ratajczak M.Z.

Poster P3

INFLUENCE OF GROWTH HORMONE ON HSC AND VSELS IN OLD C57BL6 MICE

Piotrowska K., Słuczanowska-Głąbowska S., Mierzejewska K., Suszyńska E., Tkacz M., Tarnowski M., Ratajczak M.Z.

Poster P4

MOLECULAR CHARACTERISTICS OF UMBILICAL CORD BLOOD-DERIVED VERY SMALL EMBRYONIC-LIKE STEM CELLS – IMPRINTED GENES METHYLATION AND PLURIPOTENCY GENES EXPRESSION STUDIES

Tarnowski M., Czerewaty M., Mierzejewska K., Suszyńska M., Ratajczak M.Z.

Poster P5

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BIOLOGICAL FEATURES OF CORD BLOOD- DERIVED CD45-/LIN-/CD133+ VERY SMALL EMBRYONIC-LIKE STEM CELLS

Karnas E., Bobis-Wozowicz S., Madetko-Talowska A., Bik-Multanowski M., Łabędź-Masłowska A., Boruczkowski D., Madeja Z., Ratajczak M.Z., Zuba-Surma E.K.

Poster P6

IN VITRO IL23R EXPRESSION ON T LYMPHOCYTES IN PATIENTS WITH MULTIPLE SCLEROSIS IN COCULTIVATION WITH MESENCHYMAL STEM CELLS

Rudakovskaya E.B., Bagatka S.S., Yurkevich M.Yu., Zafranskaya M.M.

Oral, Poster P7

OPTIMIZATION OF TREATMENT METHODS FOR GRAFT-VERSUS-HOST DISEASE WITH MESENCHYMAL STEM CELLS CULTURED ON NANOCOATINGS

Goltsev A.N., Rassokha I.V., Dubrava T.G., Ostankova L.V., Ostankov M.V.

Oral, Poster P8

SYNGENEIC TRANSPLANTATION OF MESENCHYMAL STEM CELLS INDUCES ACUTE INFLAMMATORY RESPONSE

Gala K., Burdzińska A., Idziak M., Pączek L.

Oral, Poster P9

POTENTIAL CLINICAL ASPECTS OF THE USE OF MESYNCHYMAL STEM CELLS INTRAPORTAL INJECTION FOR MINIMIZATION OF THE ISCHEMIA REPERFUSION INJURY IN A RAT MODEL - PRELIMINARY STUDY

Nowacki M., Nazarewski Ł., Judziłł A., Tyloch D., Kloskowski T., Pietkun K., Bodnar M., Marszałek A., Gagat M.,Grzanka A., Pokrywczyńska M., Krawczyk A., Drewa T.

Oral, Poster P10

PHOSPHATIDYLSERINE EXPOSURE AND BAX PROTEIN EXPRESSION IN PORCINE MESENCHYMAL STEM CELLS SUBJECTED TO HIGH HYDROSTATIC PRESSURE - PRELIMINARY RESULTS

Romanek J., Opiela J., Smorąg Z. Poster P11

TISSUE DISTRIBUTION, PREPARATION AND PHENOTYPE CHARACTERISTICS OF STEM CELLS HARVESTED FROM DIFFERENT HUMAN TISSUES

Klimczak A., Jurek T., Rodziewicz A., Czuba M., Rorat M., Kozłowska U., Lange A.

Poster P12

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IDO-DEPENDENT MECHANISM OF MOUSE FETAL LIVER MESENCHYMAL STEM CELLS IMMUNOCORRECTING ACTIVITY

Dimitrov A., Dubrava T., Goltsev A.

Poster P13

NOVEL IN VIVO EVIDENCE THAT NOT ONLY ANDROGENS BUT ALSO PITUITARY GONADOTROPINS AND PROLACTIN DIRECTLY STIMULATE MURINE BONE MARROW STEM CELLS – IMPLICATIONS FOR POTENTIAL TREATMENT STRATEGIES IN APLASTIC ANEMIAS

Mierzejewska K., Suszyńska E., Borkowska S., Suszyńska M., Maj M., Ratajczak J., Kucia M., Ratajczak M.Z.

Oral, Poster P14

PORCINE MESENCHYMAL STEM CELL STAINING BY FE NANOPARTICLES AS A PREPARATION FOR VISUALISING IN LIVER REGENERATION

Miklikova M., Liska V.,Holubova M., Lysak D., Mirka H., Kralickova M., Vistejnova L.

Oral, Poster P15

ACTIVATION OF BONE MARROW-DERIVED VERY SMALL EMBRYONIC-LIKE STEM CELLS BY ACUTE TISSUE INJURY IN VIVO

Łabędź-Masłowska A., Karnas E., Berdecka D., Brzozowski T., Ratajczak M.Z., Madeja Z., Zuba-Surma E.K.

Oral, Poster P16

RECOVERY OF MOTILITY IN RATS WITH SPINAL CORD INJURY AFTER TREATMENT WITH VEGF+ MESENCHYMAL STEM CELLS

Duruksu G., Kabatas S., Okcu A., Civelek E., Subasi C., Turac G., Yilmaz I., Karaoz E.

Oral, Poster P17

APPLICATION OF CRYOPRESERVED EMBRYONIC FIBROBLASTS ON CARRIERS FOR TREATMENT OF ASEPTIC DERMAL INFLAMMATION IN RATS

Abrafikova L.G. Oral, Poster P18

THE INFLUENCE OF CRYOPRESERVATION ON THE EXPRESSION LEVEL OF STEMNESS-GENES IN MOUSE FETAL LIVER CELLS

Borysov P., Dimitrov A., Goltsev A.

Oral, Poster P19

NEW INSIGHT INTO ENDOTHELIAL PROGENITOR CELLS PARACRINE, PROANGIOGENIC PROPERTIES

Kantor A., Paprocka M., Bielawska-Pohl A., Krawczenko A., Grillon C., Kieda C., Duś D.

Oral, Poster P20

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BOTH COMPLEMENT AND COAGULATION CASCADES ARE ACTIVATED IN HEALTHY VOLUNTEERS IN CIRCADIAN RHYTHM-DEPENDENT MANNER – IMPLICATION FOR STEADY STATE CIRCULATION OF STEM CELLS. PRELIMINARY REPORT

Budkowska M., Sałata D., Matoszka N., Jędrzychowska A., Ratajczak M.Z., Dołęgowska B.

Poster P21

STEM CELLS IN DERMATOLOGICAL FILLERS- IT'S A GOOD CONCEPT? A PRELIMINARY REPORT OF ANIMAL STUDY

Pietkun K., Nowacki M., Pokrywczyńska M., Kloskowski T., Rasmus M., Warda K.,Gagat M., Grzanka A., Bodnar M., Marszalek A.,Czajkowski R., Drewa T.

Poster P22

REGENERATIVE-PLASTIC POTENTIAL OF CRYOPRESERVED CORD BLOOD SERUM IN GONARTHROSIS

Vvedenskiy B.P., Kovalyov G.A., Dedukh N.V., Sandomirskiy B.P.

Poster P23

PERSPECTIVE OF APPLYING CRYOPRESERVED CORD BLOOD FOR INFLUENZA PREVENTION (EXPERIMENTAL STUDY)

Goltsev A.N., Kozhyna O.Yu., Ostankova L.V., Bondarovich N.A.

Poster P24

EXPERIMENTAL SUBSTANTIATION FOR CRYOPRESERVED CORD BLOOD APPLICATION IN COMBINED THERAPY OF ACUTE PURULENT PERITONITIS

Goltsev K.A., Krivoruchko I.A., Azhgibesov K.A., Ostankov M.V., Ostankova L.V., Goltsev A.N.

Poster P25

EXPERIMENTAL SUBSTANTIATION OF POSSIBLE APPLICATION OF FETAL LIVER CRYOPRESERVED CELLS TO TREAT ATOPIC DERMATITIS

Nosenko L.A., Ostankov M.V., Bondarovich N.A., Ostankova L.V., Goltsev A.N.

Poster P26

CAN SOMATIC STEM CELLS BE RESPONSIBLE FOR REGENERATIVE PROPERTIES OF ARTICULAR CARTILAGE?

Kochanowska I.E., Śmieszek A., Wysocka-Wycisk A., Bursig H.

Poster P27

RAT CARTILAGE REPAIR USING COLLAGEN GEL AND BONE MARROW MESENCHYMAL STEM CELLS, THE IN VIVO PRELIMINARY STUDY

Bajek A., Skopińska-Wiśniewska J., Rynkiewicz A., Jundziłł A., Nowacki M., Drewa T.

Poster P28

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APPLICATION OF CRYOPRESERVED CORD BLOOD FOR CORRECTION OF IMMUNE SYSTEM IN MODEL SYSTEM OF ISCHAEMIC STROKE

Lebedinets V.V., Ostankov M.V., Lebedinets D.V., Goltsev A.N.

Poster P29

CAPRINE MUSCLE-DERIVED CELLS AND BONE MARROW MESENCHYMAL STEM CELLS - ISOLATION AND IDENTIFICATION FOR CELL THERAPY PURPOSES

Burdzińska A., Gilbert A., Szczepańska I., Gajewski Z., Pączek L.

Poster P30

CLINICAL AND PATHOMORPHOLOGICAL SUBSTANTIATION OF APPLICATION OF CRYOPRESERVED CORD BLOOD CELLS (CCBC) TO COMPENSATE CORNEAL LIMBAL DEFICIENCY (CLD) IN EXPERIMENT

Svidko K.M., Demin Yu.A. Poster P31

MODIFICATION OF STRUCTURAL AND FUNCTIONAL POTENTIAL OF BONE MARROW STEM CELLS OF ANIMALS WITH EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS

Porozhan Ie.A., Babenko N.N., Gayevskaya Yu.A., Dubrava T.G., Goltsev A.N.

Poster P32

MODIFYING EFFECT OF CRYOPRESERVATION ON IMMUNOMODULATORY AND ANTITUMOR ACTIVITIES OF FETAL LIVER CELLS

Goltsev A.N., Bondarovich N.A., Kuznyakov A.V., Ostankov M.V., Chelombit'ko O.V.

Poster P33

APPLICATION OF CRYOPRESERVED CORD BLOOD FOR CORRECTION OF IMMUNE SYSTEM WITH DYSFUNCTIONAL UTERINE BLEEDING CAUSED BY HERPES VIRUS INFECTION

Stetsyshyn V.G., Ostankova L.V, Goltsev A.N

Poster P34

INTRAUTERINE ADMINISTRATION OF AUTOLOGOUS PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMC) INCREASES PREGNANCY RATE IN IVF CYCLES WITH TRANSFER OF CRYOPRESERVED EMBRYOS

Petrushko M.P., Pinyaev V.I., Podufaliy V.V., Pravdina S.S.

Oral, Poster P35

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NOVEL EVIDENCE THAT CROSSTALK BETWEEN THREE EVOLUTIONARILY ANCIENT PROTEOLYTIC ENZYME CASCADES (COAGULATION, FIBRINOLYSIS, AND COMPLEMENT) PLAYS AN IMPORTANT ROLE IN MOBILIZATION OF HEMATOPOIETIC STEM/PROGENITOR CELLS (HSPCS)

Borkowska S., Suszynska M., Wysoczynski M., Mierzejewska K., Budkowska M., Salata D., Dolegowska B., Ratajczak J., Kucia M., Ratajczak M.Z.

Poster P36

STATE OF HEMOPOIETIC PRECURSORS IN BONE MARROW OF ANIMALS WITH ADJUVANT ARTHRITIS PRIOR TO AND AFTER APPLYING CRYOPRESERVED PLANCETAL CELLS

Lutsenko E.D., Dubrava T.G., Ostankov M.V., Porozhan E.A., Chelombit'ko O.V., Goltsev A.N.

Poster P37

DOES THE BIOLOGICALLY ACTIVE SUBSTANCES FROM KALANCHOE INFLUENCE THE HUMAN FIBROBLASTS PROLIFERATION, THE IN VITRO PRELIMINARY STUDIES

Porowińska D., Bajek A., Jakubowska A., Drewa T.

Poster P38

CHANGE IN CRYORESISTANCE OF BONE MARROW HEMOPOIETIC STEM CELLS AT AUTOIMMUNE PATHOLOGY

Goltsev A.N., Gaevskaya Yu.A., Ostankova L.V., Dubrava T.G.

Poster P39

INDUCED PLURIPOTENT STEM CELLS AS SOURCE OF BIOACTIVE MICROVESICLES WITH PARACRINE ACTIVITY ON MATURE TARGET CELLS

Kmiotek K., Bobis-Wozowicz S., Kołcz J., Madeja Z., Zuba-Surma E.K.

Poster P40

CLINICAL USE OF SOMATIC STEM CELLS - SINGLE CENTER EXPERIENCE

Majka M., Jarocha D., Milczarek O., Krasowska-Kwiecień A., Skotnicki S., Stangel-Wojcikiewicz K., Basta A., Musiałek P.

Poster P41

CHANGE OF MOLECULAR AND GENETIC CHARACTERISTICS OF EHRLICH CARCINOMA CELLS UNDER CRYOPRESERVATION FACTORS

Chelombytko O.V., Bondarovich N.A., Ostankov M.V., Dimitrov A.Yu., Goltsev A.N.

Poster P42

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SIGNIFICANT PROTEIN FACTOR IN DILUTED PLASMA RESPONSIBLE FOR CANCER CELLS CHEMOTACTIC POTENCY

Schneider G., Serwin K., Bryndza E., Kucia M., Ratajczak J., Poniewierska A., Tkacz M., Dołęgowska B., Ratajczak M.Z.

Poster P43

AUTOTRANSPLANTATION OF THE ADIPOSE TISSUE DERIVED STEM CELLS FOR CHRONIC WOUNDS HEALING

Masłowski L., Paprocka M., Witkiewicz W., Duś D., Grendziak R., Kubiak J., Buczyńska A., Wojtowicz-Prus E.

Poster P44

REGENERATION OF THE IMMUNE SYSTEM POST THE HSCT RELIES ON THE NUMBER OF TRANSPLANTED CD34+ CELLS

Lange J., Wołowiec D., Dłubek D., Mizia S., Lange A.

Poster P45

*please note that all authors are invited to mount their posters

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ACKNOWLEDGEMENTS

We wish gratefully acknowledge with thanks the support

offered by sponsoring and exhibiting companies

SPONSORS

Astellas Pharma MSD

Becton Dickinson Neovii Biotech formerly Fresenius Biotech GmbH

BioSpherix Pierre Fabre

Janssen-Cilag Roche

Maco Pharma Polonia Syngen Biotech

EXHIBITORS

ALAB EUMed

Amgen Gilead

Celgene Novartis

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LIST OF INVITED SPEAKERS AND CHAIRPERSONS Chevallier, Nathalie (Créteil) invited speaker; chairman

Danielewicz, Roman (Warszawa) chairman

De Havilland, Julian R. (Newcastle) invited speaker; chairman

Górski, Andrzej (Warszawa) invited speaker; chairman

Jaskuła, Emilia (Wroclaw) workshop leader

Karaoz, Erdal (Kocaeli) invited speaker; chairman

Klimczak, Aleksandra (Wroclaw) invited speaker; chairman

Krause, Diane S. (Yale) invited speaker; chairman

Kucia, Magdalena (Louisville) invited speaker; chairman

Lange, Andrzej (Wroclaw) president; speaker

Lange, Magdalena (Wroclaw) invited speaker

Lapidot, Tsvee (Israel) invited speaker

Murray, Kevin (New York) invited speaker

Ratajczak, Mariusz (Louisville, Szczecin)

invited speaker; chairman

Tonn, Torsten (Dresden) invited speaker; chairman

Witkiewicz, Wojciech (Wroclaw) Chairman

Wojakowski, Wojciech (Katowice) invited speaker

Zuba-Surma, Ewa (Krakow) invited speaker; chairman workshop leader

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PARTICIPANTS

Abrafikova, L.G. Karnas, Elżbieta Bajek, Anna Kaźmierski, Łukasz Banas, Natalia Kloskowski, Tomasz Berdecka, Dominika Kmiotek, Katarzyna Białoń, Krzysztof Kobusińśka, Magdalena Bocheńska, Jolanta Kochanowska, Iwona Ewa Bondarovich, Nikolay A. Koćwin, Edyta Borowik, Agata Kościńska, Katarzyna Borysov, Peter Kozłowska, Urszula Budkowska, Marta Krydel, Michalina Burdzińska, Anna Kwiatkowska, Małgorzata Butrym, Marta Lange, Janusz Chelombytko, Olga V. Laskowska, Anna Ciborowska, Magdalena Lebedinets, V.V. Dera-Joachimiak, Dorota Legieć, Wojciech Dimitrov, Aleksey Lutsenko, E.D. Dołęgowska, Barbara Łabędź-Masłowska, Anna Dubrava, Tatyana G. Majcherek, Maciej Duda, Dorota Makowska, Anna Dudkiewicz, Małgorzata Matoszka, Natalia Duruksu, Gokhan Mierzejewska, Katarzyna Futoma, Katarzyna Miklikova, Michaela Gaevskaya, Yu.A. Morawska, Anna Gala, Kamila Mordak-Domagała, Monika Galecka, Ewa Nazarewski, Łukasz Gilbert, Anna Nosenko, L.A. Godlewska, Urszula Nowacki, Maciej Goltsev, Anatoliiy N. Olszewska, Agnieszka Goltsev, K.A. Opiela, Jolanta Goździewicz, Tomasz Owczarek, Dorota Gruszecka-Czopek, Beata Paduch, Agnieszka Grzesiak, Jakub Pakos, Helena Jakubowska, Aneta Paprocka, Maria Janik, Katarzyna Petrushko, M.P. Jerzyk, Małgorzata Pietkun, Katarzyna Jędrzychowska, Adriana Pietraszek, Elżbieta Kantor, Aneta Piotrowska, Katarzyna Karbowska, Żaneta Piotrowska, Małgorzata

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Podufaliy, V. V. Porowińska, Dorota Porozhan, Ievgeniia Przesmycki, Jacek Rawicka, Joanna Rojna, Maciej Romanek, Joanna Rudakovskaya, Ekatherina Sałata, Daria Serwin, Karol

Sędzimirska, Mariola Siemieńska, Anna Singh, Paulina Siudy, Monika Skrzyniarz, Beata Sobas, Marta Sobczyńska-Konefał, Anna Stanik, Maciej Staniszewska, Marzena Stetsyshyn, Viktoriia G. Suchnicki, Krzysztof Svidko, Kateryna M. Szczepańska, Izabela Szulc, Patrycja Tarnowski, Maciej Torosian, Tigran Troczyńska, Magdalena Vistejnova , Lucie Vvedenskiy, Boris P. Walter-Croneck, Adam Wanecka, Anna Wrzeszcz, Karol Zarychta, Weronika Zdziarski, Przemysław Zyuz'kov, G.N.

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