Computer Assisted Semen Analysis - nour-eleslam.com · Integrated Sperm Chemistry Analyzer. Computer Assisted Semen Analysis System utilizesmodern computer and advanced image processing

Computer AssistedSemen Analysis

Touch smart interface

Soft/Hard copy Reports

Wi-Fi Tablet Support

Bar-Code Support

Integrated Sperm Chemistry Analyzer

Computer Assisted Semen Analysis System utilizes modern computer and advanced image processing techniques for clinical test of sperm motility, quality, morphology, vitality, DNA fragmentation, HOS and nuclear protein. Based on the sperm test standards of the WHO 5th criteria (2010), the system can analyze the characteristic of sperm comprehensively through image processing of sperms. The system analyzes these values and generates accurate parameters to reflect sperms’ quality. The whole procedure is fast and provides several important information that are vital to present scientific basis for the male reproductive ability.

Spermolyzer

NATURALLY SHINE

1- Dynamic standard method :• The method is designed to estimate the spermatozoa concentration and their motility in a native specimen. • Determining spermatozoa concentration in ( million/ml)• Measuring spermatozoa movement parameters and classify this movement to classes in accordance of WHO

recommendations .• Measuring spermatozoa dynamic parameters ( VCL/VSL/VAP/MAD/ALH/BCF/LIN/WOB/STR) 2- Morphology standard method:• The method is designed to determine the morphological parameters of Spermatozoa.• The software calculate the ( TZI/SDI/MAI) according to the latest criteria Of the world health organization.

3- Vitality test:This test is important to determine if the non-motile spermatozoa are alive or dead. It should be assessed if a low percentage of sperm are progressively motile, e.g., 30 - 40%.The two most common methods of sperm vitality assessment: • Eosin-nigrosin staining. • The hypo-osmotic swelling test ( HOS).

4- Sperm DNA Fragmentation Test:This test provides a reliable analysis of sperm DNA integrity that may help to identify men who are at risk of failing to initiate a healthy ongoing pregnancy. Information about sperm DNA integrity may help in the clinical diagnosis, management and treatment of male infertility and may be of prognostic value in assessing outcome of assisted conception treatment.

5- HOS: The hypo-osmotic swelling test is based on the semi-permeability of the intact cell membrane, which causes spermatozoa to “swell” under hypo-osmotic conditionsAll the curled tail sperm are labeled as ‘Live Sperm’. The damaged / brokensperm membrane, allows fluids to pass across the membrane without anyaccumulation & hence there is No Cytoplasmic Swelling & the tail remains straight(No Curling). All these sperm are labeled as the Dead sperm.

6- Nuclear Protein Assay:The mature chromatin contains around 85% Protamine & 15% Histone, whereas immature chromatin contains more than 15% Histone.Increased levels of persistent Histone in turn indicate a break in the development sequence of Histone – Transition Protein – Protamines, which significantly affect the DNA chain folding & vulnerability for increased DNAFragmentation & this leads to decline in sperm fertilizing potential. Hence by evaluating Nucleoprotein assessment the diagnosis & prognosis of Male Infertility can be determined.

Creative Constant-Temperature Operation DeskThe operation desk makes provides a constant temperature of 37 during sperm testing during the whole test procedure. This in turn eliminates the influence of excessive low outside temperature on the tested sperm such as sperm velocity, sperm vitality etc. Unique Virtual Grid FunctionThis function has realized the direct comparison of test results between human being and machine in the system. If you wish to use manual counting there is no need to buy an expensive calibrated counting chamber, it is all in your screen with the aid of virtual grid function

Database:• The in-built database enable the user to store, backup and restore any kind Of obtained information .• The in-built database enable the user to generate historical report for any Patient.• The in-built database is capable of helping researcher by generating many Statistics reports.

Other Features of Spermolyzer software:• Spermolyzer software is able to automatically add appropriate comments according to the patient results.• Spermolyzer software has the ability to generate accustom reports like Urine, Stool, CBC and Bone marrow

with any captured image using the system digital camera and microscope.•Spermolyzer software allows the user to record and print the manual test result of semen.

Standard Methods:

• Spermolyzer includes two standard methods ( Dynamic and Morphology)

• The analysis of the parameters is performed according to the recommendations given by The

world health organization (WHO) for the latest 5th edition for motility patterns & Morphometric

assessment of human semen.

System Requirements

Computer

Operating system

Processor

HDD

Ram

Display Adapter

Slide

Camera

Heater

Microscope

Desktop, Laptop or All in one PC

Windows 7 or 8.1 (32 or 64 bits)

Intel core i3 or Higher

1 TB or Higher

4 GB DDR3 or Higher

ntel Integrated graphics or ATi or nvidia 1GB

Special Slide for count and motility

USB 3.0 industrial CMOS digital camera 30 frame /sec640 x 480 minimum resolution

Special temperature controller with attached heateron the microscope stage

Olympus, Micros, Nikon, Zeiss, Leica C-mount 1x, Trinocular tube 1x,Objective : 10x, 20x, 40x, 100x oil plan achromatic lens.

Sperm Vitality

Kit

Andrology Solution

Kit

Sperm Function Test Kit

Spermolyzer (Smart CASA

system)

Viscosity Reagnent

Kit

Sperm Fructose Kit

Sperm pH strips Sperm

HOS reagent

Kit

Sperm Morphology

Kit

Sperm Vitality

TestSperm

Dynamic Test

Sperm Morphology

Test

HOSTest

DNA fragmentation

Test

Zink determination

kits

Nuclear protein

assessment kits

Antisperm antibody

(IgG & IgA) Reagent

Kits

–Glycosidase

kits

Leukocytes Confirmation

kits

DNAfragmentation

reagent Kit

Mediumfor spermSwim up

technique

Semen processing

solutions

Mediumfor sperm

Swim down technique

IUI Catheter.

Non- Spermicidal

semen collection

pouch

Andrology lab will be most Easy and Practical….With specially developed instruments & accessories for all sperm function tests considering their specific

functional and ergonomical needs.The instruments are developed considering in vivo temperature environment of the natural processes,

Where ever needed which brings systematic approach and give quality test results.

Spermolyzer (Smart CASA system):Spermolyzer is a computer assisted semen analysis System which utilizes modern computer and advanced image processing techniques for clinical test of sperm based on the standards of the WHO 5th strict criteria.

Spermolyzer Software support:* Sperm Dynamic Test. * Sperm Morphology Test.

* DNA fragmentation Test. * HOS Test.

* Sperm Vitality Test.

Warmer for Semen specimen:Semen specimen warmer is a temperature control system which is ergonomically designed to give ease of work and specimen handling. The system has heater & sensor programmed to attain & maintain 37°C. It has very high accuracy with ± 0.20C.

Warmer for DNA fragmentation Test.: DNA fragmentation test warmer is a dual temperature control system, designed to expose the agarose tubes at 70°C or at 37°C in separate blocks of instrument. The two blocks for two different temperatures can be used simultaneously.You can thereby cut off the hassle included in conventional method and do the work in ease in just one instrument.Also, Its ergonomics allows user to handle each tube without disturbing others.

Sperm Centrifuge 37°C: Sperm centrifuge machine designed to work at 37°C, that is same as the in-vivo temperature, to maintain the natural functionality of sperm.

Technical specifications: •Maximum speed: 4000 RPM (with rotor 4x 15ml.)

•Maximum RCF: 2,719 X g (with rotor 4x 15ml.)

•Maximum sample volume: 60 ml.

•Speed setting & display: Digital.

•Speed accuracy :± 100RPM.

MIRALAB ANDROLOGY LINE

Normal seminal profile

223Patient Name

Dr.\Galal Ibrahimص 10:31:13 25/02/2015201502251 Name

Lab ID

The system follows WHO (2010) strict criteria for motility patterns & morphometric assessment of human semen.

Sem

en S

cree

n (C

ompu

ter

Ass

iste

d Sem

en A

naly

sis)

Semen Screen (Computer Assisted Semen Analysis)

_

0

20 _

40 _

60

80

100

_

_

54.38

35.48

NP

10.14

IMPR

Case no. Date

Ref. by

Collection Abestination

Physical Proerties

pH Volume

Color Odor Viscosity Liquefaction time Liquefaction state

47.7Grey OpalescentNormalNormal20Complete

Test Result Test Concentration Total sperm count Progressive motility (PR)

Masturbation at lab4

Result Status Reference value

Total motility (PR+NP) Morphology index Vaitality

54.15216.654.3889.86

2463

PassedPassedPassedPassedPassedPassed

>= 15 (million/ml) >= 39 (million/ejaculate) >= 32 % >= 40 % >= 4 % >= 58 %

Agglutination & Aggregation Agglutination Agglutination grade Non-specific aggregation

Head to headIsolated ( < 10 spermatozoa per agglutinate, many free spermatozoa )Between active sperm and mucus thread

Other Cells White blood cells Red blood cells Spermatogenic cells Epithelial cells

Comment

2 - 44 - 611 - 2

The semen passed the W.H.O. strict criteria for count, motility and morphology.

Pinheads :

Pinheads sperms are not counted as spermatozoa orhead defects because they possess no chromatin orhead structure anterior to the basal plate.

million/ml1

>= 1.5 ml>= 7.2

Age

< 5 /H.P.F.< 5 /H.P.F./H.P.F./H.P.F.

2 - 5 days

15 - 60 min.

HOSPITEXDiagnostics

HOSPITEXDiagnostics

Italy

Tel:+202 2504 4134 Fax:+202 2504 4135 Email:[email protected] www.mira-lab.com

Building No.6105/6 Quds St, Mokattam , Cairo, EGYPT

Name

Sem

en S

cree

n (C

ompu

ter

Ass

iste

d Sem

en A

naly

sis)


Case no.

Dynamic parameters (I) Classification Tested sperms

Progressive motility (PR)

Conc. (million/ml) Total number (million) Percentage %

Total motility (PR+NP)

Non progressive (NP) Immotile (IM)

54.15

29.4548.66

19.215.49

Dynamic parameters (II)

Patient Name 201502251

Motility Report

216.6

117.8194.64

76.8421.96

100

54.3889.86

35.4810.14

Progressive motility (PR)

Non-progressive motility (NP)

Immotile (IM)

Spermatozoa moving actively either linearly or in a large circle regardless of speed

All other patterns of motility with absence of progression, i.e. swimming in small circle, the flagellarforce hardly displacing the head, or when only a flagellar beat can be observed.

No movement

VCL (µm/s) 32.44 VSL (µm/s) 16.61 VAP (µm/s) 23.33

MAD (°) 57.57 ALH (µm) 2.99 BCF (Hz) 2.93 LIN (%) WOB (%) STR (%)

VCL VSL VAPCurvilinear velocity Straight line velocity Average path velocity

MAD ALH BCFMean angular degree Amplitude of lateral head displacement Beat-cross frequency

51.21 71.91 71.05

LIN WOB STRLinearity (VSL/VCL) Wobble (VAP/VCL) Straightness (VSL/VAP)

VSL

VCL

VAP

ALH

MAD

Head displacement

Sperm tracks and velocities Legend

DCL (µm) 26.49 DSL (µm) 13.53 DAP (µm) 19.04

DCL DSL DAPDistance curved line Distance straight line Distance average path

HOSPITEXDiagnostics

HOSPITEXDiagnostics

Italy



Name

Sem

en S

cree

n (C

ompu

ter

Ass

iste

d Sem

en A

naly

sis)


Case no.

Progressive velocity (VSL)

Patient Name 201502251

Charts Report

% %

µm/sµm/s 0-10 10-20 20-30 30-40 40-50 50-60 >60

51.18

21.9810.44 10.20

4.95 0.55 0.69

100

80

60

40

20

0

_

_

_

_

_

100

80

60

40

20

0

_

_

_

_

_

Track speed (VCL)

Path velocity (VAP)

100

80

60

40

20

0

_

_

_

_

_

% %

µm/s

100

80

60

40

20

0

_

_

_

_

_

Sperm distribution

Track image (I) Track image (II)

PR

54.38

35.48

NP

10.14

IM

0-10 10-20 20-30 30-40 40-50 50-60 >60

34.60

12.53 14.019.06 7.13 5.64

17.03

0-10 10-20 20-30 30-40 40-50 50-60 >60

39.16

15.25 15.79 14.94 10.603.57 0.69

PR (Progressive) NP (Non Progressive) IM (Immotile)

HOSPITEXDiagnostics

HOSPITEXDiagnostics

Italy



Tail defectsNeck & midpiece defects

24 76

80

1612

0

00

0

00

0

00

4

0

432

52

0

0

Name

Sem

en S

cree

n (C

ompu

ter

Ass

iste

d Sem

en A

naly

sis)


Case no.Patient Name 201502251

Morphology Report

Class4.48

Head length

3.05Length/Width (%)

1.49

Head defects

Normal sperms (Morphology Index) Terato sperms

A. Head abnormalities

% %


C. Principal piece (Tail) abnormalities%%

Normal sperms (Morphology Index) lblPyriformH

%%

Normal sperms (Morphology Index) Terato sperms % Vaculated Small acrosome

%%

Double head %

Big head Small head Tapered head Pyriform head Round head Amorphous

%


%%


%%

Normal sperms (Morphology Index) Terato sperms %

Short tail Bent tail Multi tail Break-down tail Coiled tail Irregualr tail

%

D. Excess residual cytoplasm (ERC)% E. R. C.

B. Neck & Midpiece abnormalities Normal sperms (Morphology Index) Terato sperms

%%


%%

Thin neck Thick neck Sharply bent Asymmetrical insertion

MAI (Multiple Anomalies Index) :The mean number of anomalies per abnormal spermatozoon

1.68

TZI (Teratozoospermic Index) :Total number of defects divided by the number of abnormal sperms

1.32

SDI (Sperm Deformity Index) :Total number of defects divided by the number of sperms counted

1.28

Head width

Mean of normal spermsMean of terato sperms

Reference values

Head area Acrosome (%) Head perimeter

12.53 12.89 37.715.43 3.05 2 14 15.77 177

4 - 5.5 2.5 - 3.5 1.5 - 1.75 7.85 - 15.1 10.8 - 14.9 40 - 70

Tapered Pyriform Round

Amorphous Vaculated Small acrosome

Bent Asymm. insertion

Thick Thin

Short Bent

Coiled E.R.C.

HOSPITEXDiagnostics

HOSPITEXDiagnostics

Italy



Name

Sem

en S

cree

n (C

ompu

ter

Ass

iste

d Sem

en A

naly

sis)



Morphology Images

Normal sperm Thin neck Small acrosomeSmall headThin neck

Normal sperm Thick neck Small acrosomeBig head

Bent neck

Normal sperm Normal Sperm Small acrosome

HOSPITEXDiagnostics

HOSPITEXDiagnostics

Italy



Name

Sem

en S

cree

n (C

ompu

ter

Ass

iste

d Sem

en A

naly

sis)



Vitality Report

Vitality image (I) Vitality image (II)

Total sperms

Live sperms Dead sperms

954

55.5644.44

%#

100

_

0

20 _

40 _

60

80

100

_

_

Live

44.4455.56

Dead

Live Dead

HOSPITEXDiagnostics

HOSPITEXDiagnostics

Italy



Name

Sem

en S

cree

n (C

ompu

ter

Ass

iste

d Sem

en A

naly

sis)



Morphology Images

Normal sperm Thin neck Small acrosomeSmall headThin neck

Normal sperm Thick neck Small acrosomeBig head

Bent neck

Normal sperm Normal Sperm Small acrosome

HOSPITEXDiagnostics

HOSPITEXDiagnostics

Italy

Non Fragmented Fragmented Degraded

Name

Sem

en S

cree

n (C

ompu

ter

Ass

iste

d Sem

en A

naly

sis)



DNA Fragmentation Report

DNA fragmentation image (I) DNA fragmentation image (II)

Total sperms

Non fragmented Fragmented

451332

28.8971.11

%#

Without halo 9 20

%#

Small halo Medium halo 17

1437.7831.11

Big halo 1 2.22

Degraded 4 8.89

_

0

20 _

40 _

60

80

100

_

_

Non Fragmented

71.11

28.89

Fragmented

_

0

20 _

40 _

60

80

100

_

_

Big

2.22

37.78

Medium

8.89

Small

31.1120.00

Without DegradedHalo size

100

DNA Fragmentation Index

%15 > DFI15 < DFI < 3030 < DFI

Excellent fertility potential.Good fertility potential.Poor fertility potential.

DFI 28.89

Comment

The sample has a good fertility potential

HOSPITEXDiagnostics

HOSPITEXDiagnostics

Italy



Egypt & Middle East Head OfficeBuilding No.6105/6 Quds St, Mokattam , Cairo, EGYPT

Tel : +202 2504 4134 / +201150222203 Fax : +202 2504 4135

Alexandria Branch OfficeBuilding No4, Street No7 from El Naser St., Smouha, Alexandria, EGYPT

Tel : +2011 59 88 77 05

www.mi ra - l ab .comE-mail:[email protected]

Documents

Computer Assisted Semen Analysis - nour-eleslam.com · Integrated Sperm Chemistry Analyzer. Computer Assisted Semen Analysis System utilizesmodern computer and advanced image processing