COMPILATION OF IP ASSAYSgnu.inflibnet.ac.in/bitstream/123456789/2458/1/22-Ajay Patel.pdfCOMPILATION OF IP ASSAYS A ... BENZOCAINE Nitrite titration ... Determination of esters Titrant

COMPILATION OF IP ASSAYS

A

PROJECT REPORT

FOR ELECTIVE SUBJECT

SUBMITTED

TO

HEMCHANDRACHARYA NORTH GUJARAT UNIVERSITY,

PATAN.

In Partial fulfillment of the requirement

For the degree of Bachelor of

Pharmacy Year 2006-‘07

SUBMITTED BY

MR. AJAY R. PATEL

SHREE S.K.PATEL COLLEGE OF PHARMACEUTICAL

EDUCATION AND RESEARCH

GANPAT VIDYANAGAR

KHERVA - 382711 gn

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ACKNOWLEDGEMENT

I take this opportunity to express my deep sense of gratitude to

those who have helped me in this work by their help, guidance,

suggestion and encouragement. This project report has been prepared

to give a brief introduction and the project “Compilation of IP

assays” that was under taken for the fulfillment of degree course in

pharmacy. The project work has to be undertaken and completed as

per the direction of the syllabus.

I express my sincere thanks and gratitude to Mr.B.H.Patel,

who become my friend, philosopher & a guide in true sense. He

constantly helped me by suggestion, encouragement, and giving his

valuable time to discuss the progress of work at every stage.

I am grateful to our principal Dr. M. M. Patel who gave me

this golden opportunity for this work and also gave constant co-

operation and valuable guidance.

My sincere thanks to Dr. P.U. Patel, Dr. S.A. Patel, Dr.

B.G.Chaudhari, Mrs. S.K.Patel, Mrs. H.J.Panchal, Mrs. D.B.Patel

and of department of Pharmaceutical Chemistry to help me for

reference work.

I am thankful to Mr. P. I. Patel - librarian, Mahadevbhai and

Mukeshbhai who helped me in easy finding of my topic related

books & provides books to me as and when required. I Sincerely

thank to Miss Chaula Patel for their help to me.

I”m also thankful to my friends who gave me constant

encouragement & strength to complete my work.

I am sincerely thankful to all other staff members of the

college, who gave their valuable suggestion.

Last but not least thanks to my mom, dad, sister and all my

family members who are not directly interact with this work but they

are always with me at my all critical stages of this work.

Mr. Ajay R. Patel

Final B. Pharm.

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Certificate

This is to certify that the project work for elective subject entitled

“COMPILATION OF IP ASSAYS” represent the bonafide work of AJAY R.

PATEL carried out under my guidance and supervision at the department of

Pharmaceutical Chemistry, Shree S.K. Patel College of Pharmaceutical Education

and Research, Ganpat Vidyanagar during academic year 2006-2007. He has

collected the literature very sincerely and methodically. This work is up to my

satisfaction.

GUIDE

Mr.B.H. PATEL M. Pharm.

Department of Pharmaceutical Chemistry,

Shree S.K.Patel College of Pharmaceutical Education & Research,

Ganpat Vidyanagar.

HEAD OF THE DEPARTMENT

Dr. P. U. PATEL

M. Pharm., Ph. D.

Department of Pharmaceutical Chemistry,

Shree S.K.Patel College of Pharmaceutical Education & Research,

Ganpat Vidyanagar.

PRINCIPAL

Dr. M. M. Patel

M. Pharm., Ph. D., L.L.B., F.I.C. Shree S.K. Patel College of Pharmaceutical Education & Research,

Ganpat Vidyanagar.

Date :

Place :Ganpat Vidhyanagar. gnu.i

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DEDICATED TO

ALMIGHTY, MY FAMILY, TEACHERS

AND FRIENDS

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ABBERVIATIONS St.phase Stationary phase

Mb.phase Mobile phase

Amm. Ammonium

Anhyd. Anhydrous

Tab. Tablet

Inj. Injection

CONTENTS Preface

Abbreviations

monographs

A……………………………………………01

B……………………………………………05

C……………………………………………08

D…………………………………………...18

E……………………………………………23

F……………………………………………26

G……………………………………………29

H……………………………………………30

I…………………………………………….33

J…………………………………………….35

K……………………………………………35

L……………………………………………36

M……………………………………………38

N……………………………………………42

O……………………………………………46

P……………………………………………48

Q……………………………………………57

R……………………………………………58

S……………………………………………58

T……………………………………………64

U……………………………………………69

V……………………………………………69

W…………………………………………..71

X…………………………………………...71

Y……………………………………………71

Z……………………………………………71

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DRUG FORMULATION

ASSAY METHOD

REQUIREMENTS/ PARAMETERS

ALPHA AMYLASE Biochemical Method

ACEBUTOLOL HCL potentiometric

titration

titrant 0.1M NaOH

ACEBUTOLOL HCL

TABLETS

UV visible

spectroscopy

λmax

A (1%, 1 cm)

233nm

580

ACETAZOLAMIDE

ACETAZOLAMIDE

TABLETS

Non aqueous titration

A

Titrant

end-point

Tetrabutylamm. hydroxide

potentiometrically

ADRENALINE Non aqueous titration

B

Solvent

Indicator

anhyd. glacial acetic acid

crystal violet

ADRENALINE

BITARTRATE INJ.

optical rotation measured

ALBENDAZOLE Non aqueous titration

B

Solvent

Indicator


crystal violet

ALBENDAZOLE

TABLETS

UV visible

spectroscopy

λmax

A (1%, 1 cm)

309 nm

742

ALGINIC ACID Acid base back

titration

Additive

Titrant

Indicator

25 ml 0.1M NaOH

0.1M HCl

phenolphthalein

ALLOPURINOL Non aqueous titration

A

Titrant

end-point


potentiometrically

ALLOPURINOL

TABLETS

UV visible

spectroscopy

λmax

A (1%, 1 cm)

250 nm

563

ALOES UV visible

spectroscopy

λmax

A (1%, 1 cm)

500 nm (& also 440 nm)

200 at 500 nm

ALPRAZOLAM

ALPRAZOLAM

TABLETS

HPLC Mb.phase

St.phase

flow rate

detection λ

Acetonitrile +chloroform + 1-

butanol + water + glacial acetic

acid (850:80:50:20:0.5)

LC3( SS 30 cm x 4.6 mm)

2 ml per minute

254 nm

ALUMINIUM

HYDROXIDE GEL

Complexometric

titration (back)

Titrant

Indicator

0.05M lead nitrate

xylenol orange solution

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ALUMINIUM

SULPHATE

Complexometric

titration (back)

Titrant

Indicator

0.05M lead nitrate

xylenol orange solution

AMANTADINE HCL

AMANTADINE HCL

TABLETS


B

Solvent

Indicator

Anhyd. glacial acetic

acid+mercuric acetate

crystal violet

AMANTADINE HCL

CAPSULES


B

Solvent

Indicator


crystal violet

AMIKACIN Microbiological assay

AMIKACIN

SULPHATE

AMIKACIN

SULPHATE INJ.

AMILORIDE HCL

TABLETS

UV visible

spectroscopy

λmax

A(1%, 1 cm)

363 nm

692

AMILORIDE HCL Non aqueous titration

A

Solvent

End point

glacial acetic acid

+ mercuric acetate

potentiometrically

AMINOCAPROIC

ACID


B

Solvent

Indicator


crystal violet

AMINOCAPROIC

ACID TAB.

AMINOCAPROIC

ACID INJ.

AMINOPHYLLINE

TAB.

AMINOPHYLLINE

INJ.

AMINOPHYLLINE

For theophylline

Titrant

Indicator

0.1M Amm. thiocyanate

Ferric amm. sulphate

For ethylenediamine Titrant

Indicator

0.1M HCl

methyl orange

AMITRIPTYLINE HCL Non aqueous titration

B

Solvent

Indicator


crystal violet

AMITRIPTYLINE HCL

TABLETS

HPLC

Mb.phase

St.phase

flow rate

detection λ

0.03M sodium

hexanesulphonate in acetonitrile

+ water (1:1)

LC1 SS (20cm x4.6mm)

2 ml per minute

239 nm

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AMM. CHLORIDE Acid base titration Titrant

Indicator

0.1M NaOH

phenolphthalein

AMODIAQUINE HCL UV visible

spectroscopy

λmax 343 nm

AMOXYCILLIN

SODIUM INJECTION

UV visible

spectroscopy

λmax 325 nm

AMOXYCILLIN

SODIUM

Potentiometric

titration

Titrant 0.02M mercuric nitrate

AMOXYCILLIN

TRIHYDRATE

AMOXYCILLIN

TRIHYDRATE

DISPERSIBLE

TABLETS

AMOXYCILLIN

TRIHYDRATE

CAPSULES

AMPICILLIN

CAPSULES

UV visible

spectroscopy

λmax 325 nm

AMPICILLIN

DISPERSIBLE TAB.

AMPHOTERICIN B microbiological Assay

AMPICILLIN SODIUM Potentiometric

titration

Titrant 0.02M mercuric nitrate

AMPICILLIN

TRIHYDRATE

AMPICILLIN

ANALGIN TAB. Iodometry Titrant

color change

0.05M iodine

yellow colour

ANALGIN Iodometry Titrant

color change

0.05M iodine

yellow colour

ANHYD. CITRIC

ACID

Acid base titration Titrant

Indicator

1M NaOH

phenolphthalein

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ANTICOAGULANT

CITRATE DEXTROSE

SOL.

For sodium citrate-

Acid base titration

Titrant

end-point

1.3M HCl

potentiometrically

For free citric acid-

Acid base titration

Titrant

Indicator

1M NaOH

phenolphthalein

For dextrose-

Polarimetry

ANTICOAGULANT

CITRATE

PHOSPHATE

DEXTROSE SOL.

For sodium citrate-

UV visible

spectroscopy

λmax 425 nm

For free citric acid-

Acid base titration

Titrant

Indicator

1M NaOH

phenolphthalein

For sodium acid

phosphate- UV

visible spectroscopy

λmax 660 nm

For dextrose- Polarimetry

AQUEOUS

CALAMINE CREAM

Gravimetry

ASCORBIC ACID INJ. Acid base back

titration

Titrant

Color change

2,6-dichloro

phenolindophenol

pink colour

ASCORBIC ACID

TAB.

Redox titration Titrant

Indicator

0.1M ceric amm.sulphate

ferroin sulphate sol

ASCORBIC ACID Iodometry Titrant

Indicator

0.05M iodine

starch

ASPARTAME Non aqueous titration

B

Solvent

Indicator


crystal violet

ASPIRIN AND

CAFFEINE TAB.

For aspirin-- Acid

base titration

Titrant

Indicator

0.1M NaOH

phenolphthalein

For caffeine-

UV visible

spectroscopy

λmax

A(1%, 1 cm)

273nm

504

ASPIRIN TAB. Acid base back

titration

Titrant

Indicator

0.5M HCl

phenol red ASPIRIN

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ASTEMIZOLE TAB. UV visible

spectroscopy

λmax

286 nm

ASTEMIZOLE

ATENOLOL TAB. UV visible

spectroscopy

λmax

A(1%, 1 cm

275nm

53.7

ATENOLOL Non aqueous titration

A

Solvent

End point


potentiometrically ATROPINE

METHONITRATE

ATROPINE

SULPHATE EYE

OINTMENT

GC

Packed column

glass(1.5 m x 4 mm) packed

with silanised diatomaceous 80

to 100# coated with phenyl

methyl silicone ATROPINE

SULPHATE INJ.

ATROPINE

SULPHATE TAB.

ATROPINE

SULPHATE


A

Solvent

End point


potentiometrically

BACITRACIN ZINC Microbiological assay of antibiotics - Method A

BACITRACIN

BARIUM SULPHATE

FOR SUSPENSION

Gravimetry

BARIUM SULPHATE

BECLOMETHASONE

DIPROPIONATE

INHALER

UV visible

spectroscopy

λmax 525 nm

BECLOMETHASONE

DIPROPIONATE

Assay of steroids

BELLADONNA DRY

EXTRACT


Indicator

0.05M NaOH

methyl red

BELLADONNA HERB

BENZALKONIUM

CHLORIDE SOL.


Color change

0.05M potassium iodate

pale brown

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BENZATHINE

PENICILLIN INJ.

BENZATHINE

PENICILLIN TAB.

BENZATHINE

PENICILLIN

For penicillins-

Potentiometric

titration

Titrant

End point

0.02M mercuric nitrate

potentiometrically

For N,N'

Dibenzylethylenedia

mine-------


B

Solvent

Indicator

anhyd.glacial acetic acid

naphtholbenzein

BENZHEXOL HCL

TAB.

HPLC

Mb.phase

St.phase

flow rate

acetonitrile+water+

triethylamine +phosphoric acid

(800:200:0.2)

LC1( SS 15 cm x 3.9 mm)

2 ml per minute

BENZHEXOL HCL Non aqueous titration

B

Indicator 1-naphtholbenzein sol.

BENZOCAINE Nitrite titration

BENZOIC ACID Acid base titration Titrant

Indicator

0.5M NaOH

phenolphthalein

BENZOIN Acid base titration Titrant

Indicator

0.1M NaOH

phenol red

BENZYL BENZOATE

APPLICATION

BENZYL BENZOATE

Determination of

esters

Titrant 0.5M ethanolic potassium

hydroxide

BENZYL-PENICILLIN

INJ.

UV visible

spectroscopy

λmax

325nm

BENZYLPENICILLIN

POTASSIUM

BENZYLPENICILLIN

SODIUM

Potentiometric

titration

Titrant

End point


potentiometrically

BETAMETHASONE

SODIUM PHOSPHATE

INJ.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

241nm

391

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BETAMETHASONE

SODIUM PHOSPHATE

TAB.

HPLC

Mb.phase

St.phase

flow rate

citro-phosphate buffer pH 5.0 +

methanol(55:45)

LC1( SS 20cm x 4.6 mm)

2 ml per minute

BETAMETHASONE

SODIUM PHOSPHATE

UV visible

spectroscopy

λmax

A(1%, 1 cm)

241nm

297

BETAMETHASONE

TAB.

HPLC

Mb.phase

St.phase

flow rate

Detection λ

methanol + water (47:53)

LC1( SS 20 cm x 5 mm)

1.4 ml per minute

238nm

BETAMETHASONE

VALERATE

OINTMENT

BETAMETHASONE

VALERATE

HPLC

Mb.phase

St.phase

flow rate

acetonitrile + water (60:40)

LC1( SS 30 cm x 4.0 mm)

1.2 ml per minute

240nm

BETAMETHASONE Assay of steroids

BISACODYL

SUPPOSITORIES


A

Solvent

End point


potentiometrically

BISACODYL TAB. UV visible

spectroscopy

λmax

A(1%, 1 cm)

264nm

148

BISACODYL Non aqueous titration

A

Solvent

End point


potentiometrically

BISMUTH

SUBCARBONATE

Complexometric

titration

Titrant

Indicator

0.1M disodium edetate

xylenol orange

BLEOMYCIN

SULPHATE

Microbiological assay

BORIC ACID Acid base titration Titrant

Indicator

1M NaOH

phenolphthalein sol.

BROMHEXINE HCL

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

317nm

87

BROMHEXINE HCL Acid base titration Titrant

End point

0.1M NaOH

potentiometrically

BROMOCRIPTINE

MESYLATE

CAPSULES

UV visible

spectroscopy

λmax

305 nm

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BROMOCRIPTINE

MESYLATE TAB.

UV visible

spectroscopy

λmax

305 nm

BROMOCRIPTINE

MESYLATE


A

Solvent

End point


potentiometrically

BUPIVACAINE HCL

INJ.


B

Solvent

Indicator


crystal violet.

BUPIVACAINE HCL Acid base titration Titrant

End point

0.1M ethanolic NaOH

potentiometrically

BUPRENORPHINE

HCL INJ.

UV visible

spectroscopy

λmax

460 nm

BUPRENORPHINE

HCL TAB.

UV visible

spectroscopy

λmax

530 nm

BUPRENORPHINE

HCL


B

Solvent

Indicator


crystal violet.

BUSULPHAN TAB. Acid base titration Titrant

Indicator

0.1M NaOH

phenolphthalein

CAFFEINE Non aqueous titration

A

Solvent

End point


potentiometrically

CALAMINE Acid base titration Titrant

Indicator

1M NaOH

methyl orange

Ca.carbonate---


A

Solvent

End point


potentiometrically

CALCIFEROL

CAPSULES

CALCIFEROL INJ.

CALCIFEROL ORAL

SOL

CALCIFEROL TAB.

UV visible

spectroscopy

λmax 500 &550 nm.

CALCIUM

CARBONATE

CALCIUM CHLORIDE

Complexometric

titration

Titrant

Indicator

Color change

0.05M disodium edentate

calcon mixture

pink to blue

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CALCIUM FOLINATE

INJ.

CALCIUM FOLINATE

HPLC

Mb.phase

St.phase

flow rate

detection λ

Acetonitrile + tetrabutyla-

mmonium hydroxide+ water

(125:15:825)


1 - 2 ml per minute

254nm

CALCIUM

GLUCONATE INJ.

CALCIUM

GLUCONATE TAB.

CALCIUM

GLUCONATE

CALCIUM LACTATE

TAB.

CALCIUM LACTATE

CALCIUM

LEVULINATE INJ.

CALCIUM

LEVULINATE

Complexometric

titration

titrant

indicator


mordant black II

CALCIUM

PANTOTHENATE


A

Solvent

End point


potentiometrically

CAPTOPRIL TAB. HPLC Mb.phase

St.phase

flow rate

detection λ

Methanol + water + phosphoric

acid (55:45:0.05)

LC1( SS 25 cm x 4.6 mm)

1 ml per minute

220nm

CAPTOPRIL Iodometric titration Titrant

Color change


pale brown

CARBAMAZEPINE

TAB.

CARBAMAZEPINE

UV visible

spectroscopy

λmax A(1%, 1

cm)

285nm

490

CARBENICILLIN

SODIUM INJ.

CARBENICILLIN

SODIUM

Method A microbiological Assay of antibiotics

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CARBENOXOLONE

SODIUM TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

256nm

199

CARBENOXOLONE

SODIUM


B

Titrant

Indicator


thymol blue sol.

CARBIDOPA Non aqueous titration

A

Solvent

End point


potentiometrically

CARBIMAZOLE TAB.

CARBIMAZOLE

UV visible

spectroscopy

λmax

A(1%, 1 cm)

291nm

557

CEFADROXIL

CAPSULES

CEFADROXIL ORAL

SUSPENSION

CEFADROXIL TAB.

CEFADROXIL

HPLC Mb.phase

St.phase

flow rate

detection λ

acetonitrile + phosphate buffer

pH 5.0 (4:96)


1.5 ml per minute

230nm

CEFAZOLIN SODIUM

INJ.

CEFAZOLIN SODIUM

HPLC Mb.phase

St.phase

flow rate

detection λ

acetonitrile + phosphate buffer

pH 3.6 (1:9)


1.5 ml per minute

254 nm

CEFOTAXIME

SODIUM INJ.

CEFOTAXIME

SODIUM

HPLC Mb.phase

St.phase

flow rate

detection λ

KH2PO4 (60 mg)+ 120 mg

Na2HPO4 in water +Methanol

(1000:120)

LC1( SS 30 cm x 3.9 mm)

1.5 ml per minute

254 nm

CEFTAZIDIME INJ.

CEFTAZIDIME

HPLC Mb.phase

St.phase

flow rate

detection λ

Acetonitrile + phosphate buffer

pH 7.0 + water (20:100:880)

LC1( SS 15 cm x 4.6 mm)

2 ml per minute

254 nm

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CEFUROXIME

SODIUM INJ.

CEFUROXIME

SODIUM

HPLC Mb.phase

St.phase

flow rate

detection λ

Acetate buffer pH 3.4 +

Acetonitrile (100:10)

LC1( SS 25 cm x 4.6 mm)

1 ml per minute

280 nm

CELLULOSE

ACETATE

PHTHALATE

For acetyl groups-

Acid base back

titration

Titrant

End point

0.1M HCl

pink color disappers

For hydrogen

phthaloyl groups-

Acid base back

titration

Titrant

End point

0.1M NaOH

pink

CEPHALEXIN

CAPSULES

CEPHALEXIN ORAL

SUSPENSION

CEPHALEXIN TAB.

CEPHALEXIN

CEPHALORIDINE INJ.

CEPHALORIDINE

Iodometric titration

(back)

Titrant

Indicator

0.02M Na thiosulphate

starch

CETRIMIDE Redox titration Titrant

End point

0.05M potassium iodate brown

colour disappears

CHLORAMBUCIL

TAB.

HPLC Mb.phase

St.phase

flow rate

detection λ

Acetonitrile + KH2PO4

(60:40)

LC1( SS 25 cm x 4.6 mm)

2 ml per minute

254 nm

CHLORAMBUCIL Acid base titration Titrant

Indicator

0.1M NaOH

phenolphthalein

CHLORAMPHENICOL

CAPSULES

CHLORAMPHENICOL

EYE DROPS

UV visible

spectroscopy

λmax

A(1%, 1 cm)

278 nm

297

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CHLORAMPHENICOL

EYE OINTMENT

UV visible

spectroscopy

λmax

A(1%, 1 cm)

278 nm

297

CHLORAMPHENICOL

PALMITATE ORAL

SUSPENSION

CHLORAMPHENICOL

PALMITATE

UV visible

spectroscopy

λmax

A(1%, 1 cm)

271 nm

178

CHLORAMPHENICOL

SODIUM SUCCINATE

INJ.

CHLORAMPHENICOL

SODIUM SUCCINATE

UV visible

spectroscopy

λmax

A(1%, 1 cm)

276 nm

220

CHLORAMPHENICOL UV visible

spectroscopy

λmax

A(1%, 1 cm)

278 nm

297

CHLORBUTOL Redox titration Titrant

Indicator

End point

0.1M amm. thiocyanate

ferric amm. sulphate sol.

reddish yellow colour

CHLORDIAZEPOXID

E TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

308 nm

327

CHLORDIAZEPOXID

E

CHLORHEXIDINE

GLUCONATE SOL.


A

Solvent

End-point

anhyd. Glacial acetic acid

Potentiometrically

CHLOROCRESOL Iodimetric titration Titrant

Indicator

0.1M sodium thiosulphate

starch

CHLOROQUINE

PHOSPHATE INJ.

CHLOROQUINE

PHOSPHATE TAB.

CHLOROQUINE

PHOSPHATE

CHLOROQUINE

SULPHATE INJ.

CHLOROQUINE

SULPHATE TAB.


A

Solvent

End-point


Potentiometrically

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CHLOROQUINE

SULPHATE

CHLOROQUINE

SYRUP


A

Solvent

End-point


Potentiometrically

CHLOROXYLENOL

SOL.

GC Packed column



to 100# coated with

polyethylene glycol

CHLOROXYLENOL Iodimetric titration Titrant

Indicator


starch

CHLORPHENIRAMIN

E MALEATE INJ.

CHLORPHENIRAMIN

E MALEATE TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

265 nm

212

CHLORPHENIRAMIN

E MALEATE


A

Solvent

End-point

anhyd. Glacial acetic acid +

acetic anhydride

Potentiometrically

CHLORPROMAZINE

HCL INJ.

CHLORPROMAZINE

HCL TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

254 nm

915

CHLORPROMAZINE

HCL


B

Solvent

Indicator

200 ml acetone+ 15 ml mercuric

acetate

ethyl orange in acetone

CHLORPROPAMIDE

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

232 nm

598

CHLORPROPAMIDE Acid base titration Titrant

Indicator

0.1M NaOH

phenolphthalein

CHLORTHALIDONE

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

275 nm

57.4

CHLORTHALIDONE Non aqueous titration

A

Titrant

end-point


potentiometrically

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CHOLECALCIFEROL HPLC Mb.phase

St.phase

Flow rate

hexane +1-pentanol (997:3)

LC3( SS 25 cm x 4.6 mm)

2.0 ml per minute

254 nm

CHORIONIC

GONADOTROPHIN

INJ.

CHORIONIC

GONADOTROPHIN

Biological assay of chorionic gonadotrophin

CIMETIDINE TAB.

CIMETIDINE


A

Solvent

End-point


Potentiometrically

CIPROFLOXACIN

HCL TAB.

CIPROFLOXACIN

HCL

CIPROFLOXACIN INJ.

CIPROFLOXACIN

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

Phosphoric acid (with

Triethylamine) +Acetonitrile

(87:13)

LC1 ( SS 25 cm x 4 mm)

1.5 ml per minute

278 nm

CISPLATIN INJ. Acid base titration Titrant

Indicator

0.1M NaOH

phenolphthalein

CISPLATIN HPLC Mb.phase

St.phase

Flow rate

Detection λ

methanol + normal saline

(90:10)

anion-exchange silica gel (10

µm)( SS 25 cm x 4.6 mm)

1.2 ml per minute

220 nm

CITRIC ACID

MONOHYDRATE


Indicator

0.1M NaOH

phenolphthalein

CLOFAZIMINE

CAPSULES

UV visible

spectroscopy

λmax

A(1%, 1 cm)

491 nm

650

CLOFAZIMINE Non aqueous titration

A

Solvent

End-point

acetone + chloroform

Potentiometrically

CLONIDINE HCL INJ.

CLONIDINE HCL

TAB.

UV visible

spectroscopy

λmax 420 nm

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CLONIDINE HCL Acid base back

titration

Titrant

End-point

0.1M ethanolic NaOH

Potentiometrically

CLOTRIMAZOLE

CREAM

CLOTRIMAZOLE

PESSARIES

HPLC Mb.phase

St.phase

Flow rate

Detection λ

phosphoric acid (with

triethylamine) +Acetonitrile

(30:70)

LC1( SS 20 cm x 4.6 mm)

1.5 ml per minute

215 nm

CLOTRIMAZOLE Non aqueous titration

B

Solvent

Indicator

End point


1-naphtholbenzein

green color

CLOVE OIL GC Packed column


with silanised diatomaceous

120# coated with dimethyl

silicone fluid

CLOXACILLIN

SODIUM CAPSULES

CLOXACILLIN

SODIUM INJ.

CLOXACILLIN

SODIUM SYRUP

UV visible

spectroscopy

λmax 325nm

CLOXACILLIN

SODIUM

Potentiometric

titration

Titrant

End point


Potentiometrically

CODEINE

PHOSPHATE SYRUP


Indicator

0.05M NaOH

methyl red sol.

CODEINE

PHOSPHATE


B

Solvent

Indicator

anh. Glacial acetic acid +

dioxan

crystal violet.

COLCHICINE AND

PROBENECID TAB.

For colchicines

UV visible

spectroscopy

λmax

A(1%, 1 cm)

350 nm

425

For probenecid

UV visible

spectroscopy

λmax

A(1%, 1 cm)

248 nm

332

COLCHICINE TAB. As above ‘for colchicine’

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COLCHICINE Non aqueous titration

A

Solvent

End-point

toluene + acetic anhydride

Potentiometrically

COLLOIDAL SILICON

DIOXIDE

Gravimetric titration

COMPOUND

BENZOIC ACID

OINTMENT

For benzoic acid

Acid base titration

Titrant

Indicator

0.1M NaOH


For salicylic acid

Redox titration

Titrant

Indicator


starch

COMPOUND

BENZOIN TINCTURE


Indicator

0.1M NaOH

phenol red

COMPOUND SODIUM

CHLORIDE INJ.

COMPOUND SODIUM

CHLORIDE SOL.

For sodium chloride-

FP OR

AAS method A

λmax 589 nm

For potassium

chloride -- FP OR

AAS method A

λmax 767 nm

For calcium chloride

Complexometric

titration

titrant

indicator


mordant black II

COMPOUND SODIUM

LACTATE INJ.

For sodium chloride-

FP OR

AAS method A

λmax 589 nm

For potassium

chloride -- FP OR

AAS method A

λmax 767 nm

For calcium chloride

Complexometric

titration

titrant

indicator


mordant black II

For total chlorine –

redox titration

Titrant

Indicator

End point




For lactate- Acid base

titration

Titrant

Indicator

0.1M NaOH

methyl orange

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CONCENTRATED

GLYCERYL

TRINITRATE SOL.

UV visible

spectroscopy

λmax 405 nm

CONCENTRATED

HUMAN RED BLOOD

CORPUSCLES

Determine Hb content by photometric haemoglobinometry

CONCENTRATED

VITAMIN D SOL.

Assay of vitamin D

CONCENTRATED

VITAMINS A AND D

SOL.

Assay of vitamin A, Method A

Assay of vitamin D

CORTISONE

ACETATE INJ.

CORTISONE

ACETATE TAB.

CORTISONE

ACETATE

Assay of steroids

CRESOL WITH SOAP

SOL.

Quantity measured after various extraction

CYANOCOBALAMIN

INJ.

CYANOCOBALAMIN

UV visible

spectroscopy

λmax

A(1%, 1 cm)

361 nm

207

CYCLIZINE HCL TAB. UV visible

spectroscopy

λmax

A(1%, 1 cm)

225 nm

390

CYCLIZINE HCL Non aqueous titration

A

Solvent

End-point

anh. Glacial acetic acid

+mercuric acetate sol.

Potentiometrically

CYCLOPHOSPHAMID

E INJ.

CYCLOPHOSPHAMID

E TAB.

CYCLOPHOSPHAMID

E


Indicator

End point




CYCLOPROPANE Quantity measured absorbed in liquid form.

CYCLOSERINE

CAPSULES

UV visible

spectroscopy

λmax 625 nm

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CYCLOSERINE TAB. UV visible

spectroscopy

λmax 625 nm

CYCLOSERINE Acid base titration Titrant

Indicator

0.1M NaOH

thymolphthalein

CYPROHEPTADINE

HCL SYRUP

CYPROHEPTADINE

HCL TAB

UV visible

spectroscopy

λmax

A(1%, 1 cm)

286 nm

355

CYPROHEPTADINE

HCL


B

Solvent

Indicator



crystal violet

CYTARABINE INJ.

CYTARABINE


B

Solvent

Indicator


1-naphtholbenzein sol.

DANAZOL

CAPSULES

DANAZOL

UV visible

spectroscopy

λmax 285 nm

DAPSONE TAB.

DAPSONE

Nitrite titration

Solvent

Titrant

2M HCl

0.1M sodium nitrite

DEHYDROACETIC

ACID


Indicator

0.1M NaOH

phenolphthalein

DEHYDROEMETINE

HCL INJ.

UV visible

spectroscopy

λmax 282 nm

DEHYDROEMETINE

HCL


B

Solvent

Indicator


crystal violet.

DEQUALINIUM

CHLORIDE


B

Solvent

Indicator


mercuric acetate sol.

crystal violet.

DESFERRIOXAMINE

MESYLATE INJ.

DESFERRIOXAMINE

MESYLATE


Indicator

End point

0.05M sulphuric acid

0.1M Ferric amm. sulphate

potentiometrically

DESLANOSIDE INJ.

DESLANOSIDE

UV visible

spectroscopy

λmax

490 nm

DESOXYCORTONE

ACETATE INJ.

Assay of steroids

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DESOXYCORTONE

ACETATE

Assay of steroids

DEXAMETHASONE

SODIUM PHOSPHATE

INJ.

UV visible

spectroscopy

λmax

410 nm

DEXAMETHASONE

SODIUM PHOSPHATE

UV visible

spectroscopy

λmax

A(1%, 1 cm)

240 nm

297

DEXAMETHASONE

TAB.

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

Water + methanol (53:47)

LC1(10 µm)

(SS 20 cm x 5 mm)

1.4 ml per minute

238 nm

DEXAMETHASONE Assay of steroids

DEXTRAN 110 INJ.

DEXTRAN 40 INJ.

Polarimetry

DEXTROMETHORPH

AN HYDROBROMIDE

SYRUP

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

amm. nitrate in Acetonitrile +

water (70:30) and glacial acetic

acid to pH 3.4

LC1(SS 25 cm x 4.6 mm)

1.0 ml per minute

280 nm

DEXTROMETHORPH

AN HYDROBROMIDE


End-point

0.1M NaOH

potentiometrically

DEXTROSE INJ. Polarimetry

DIAZEPAM

CAPSULES

DIAZEPAM INJ.

DIAZEPAM TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

284 nm

450

DIAZEPAM Non aqueous titration

A

Solvent

End-point


Potentiometrically

DIBASIC CALCIUM

PHOSPHATE

Complexometric

titration

Titrant

Indicator

0.1M zinc sulphate

mordant black II mixture

DIBUTYL

PHTHALATE

Determination of esters

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DICLOFENAC

SODIUM INJ.

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

Methanol + 0.1M Na acetate

(60:40)

LC2 (5 µm)

SS (12.5cm x 4.6 mm)

1.0 ml per minute

254 nm

DICLOFENAC

SODIUM TAB.

UV visible

spectroscopy

λmax 285 nm

DICLOFENAC

SODIUM


A

Solvent

End-point


Potentiometrically

DICYCLOMINE HCL

ORAL SOL.

DICYCLOMINE HCL

TAB.


Indicator

0.001M sodium dodecyl

sulphate

dimethyl yellow

DICYCLOMINE HCL Non aqueous titration

B

Solvent

Indicator



crystal violet.

DIENOESTROL TAB.

DIENOESTROL

UV visible

spectroscopy

λmax 245 nm

DIETHYLCARBAMAZ

INE CITRATE TAB.


Indicator

0.1M NaOH

bromocresol green

DIETHYLTOLUAMID

E

Determination of nitrogen

DIGITOXIN TAB. UV visible

spectroscopy

λmax 550 nm

DIGITOXIN UV visible

spectroscopy

λmax 495 nm

DIGOXIN INJ.

DIGOXIN

PAEDIATRIC SOL.

UV visible

spectroscopy

λmax 590 nm

DIGOXIN TAB. UV visible

spectroscopy

λmax 545 nm

DIGOXIN As digitoxin

DIIODOHYDROXYQU

INOLINE TAB.

Oxygen-flask method Titrant

Indicator

0.02M sod. thiosulphate

starch

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DIIODOHYDROXYQU

INOLINE


A

Solvent

End-point

anhyd. pyridine

Potentiometrically

DILOXANIDE

FUROATE TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

258 nm

705

DILOXANIDE

FUROATE


A

Solvent

Titrant

End-point

anhyd. pyridine


Potentiometrically

DILTIAZEM HCL

TAB.

DILTIAZEM HCL

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

50 volumes 0.116% w/v of d-

10-camphorsulphonic acid in

0.1M sodium acetate (pH 6.2 set

with 0.1M sodium hydroxide) +

25 volumes acetonitrile + 25

volumes methanol

LC1 SS ( 30cm x 3.9 mm)

1.6 ml per minute

240 nm

DILUTE HCL Acid base titration Titrant

Indicator

1M NaOH

methyl red

DILUTED

ISOSORBIDE

DINITRATE

UV visible

spectroscopy

λmax

405 nm

DILUTED

PENTAERYTHRITOL

TETRANITRATE

UV visible

spectroscopy

λmax

409 nm

DIMERCAPROL INJ. Iodometric titration Titrant 0.05M iodine

DIMERCAPROL Iodometric titration Titrant 0.1M sodium thiosulphate

ACTIVATED

DIMETHICONE

For polydimethy-

lsiloxane

IR spectroscopy

λmax 7.9 µ

For silicon dioxide - Gravimetric titration

DIPHENHYDRAMINE

HCL CAPSULES


Indicator

0.1M NaOH

methyl red sol.

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DIPHENHYDRAMINE

HCL


B

Solvent

Indicator

anh. Glacial acetic acid + 20 ml

mercuric acetate sol

crystal violet

DIPHENOXYLATE

HCL


End point

0.1M ethanolic NaOH

Potentiometrically

DISODIUM EDETATE

INJ.

DISODIUM EDETATE

Complexometric

titration

Titrant

Indicator

2M HCl

0.1M lead nitrate

DISULFIRAM TAB. UV visible

spectroscopy

λmax 400 nm

DISULFIRAM Redox titration Titrant

End-point

0.1M AgNO3

Potentiometrically

DITHRANOL

OINTMENT

HPLC

Mb.phase

St.phaseFlow

rate

Detection λ

Hexane + Dichloromethane +

glacial acetic acid(82:5:1)


2ml per minute

354 nm

DITHRANOL Non aqueous titration

A

Titrant

Solvent

End-point


anhyd. pyridine

Potentiometrically

DOTHIEPIN HCL

CAPSULES

DOTHIEPIN HCL


B

Solvent

Indicator

acetone+mercuric acetate

methyl orange in acetone

DOXEPIN HCL

CAPSULES

UV visible

spectroscopy

λmax

297 nm

DOXEPIN HCL As dothiepin HCl capsules

DOXORUBICIN HCL

INJ.

DOXORUBICIN HCL

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

sodium dodecyl sulphate +

phosphoric acid +acetonitrile +

methanol

(50:50:45:5)

LC1(5µm)

SS (15 cm x 4.6 mm)

0.8 ml per minute

254 nm

DOXYCYCLINE HCL

CAPSULES

Microbiological assay of antibiotics

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DOXYCYCLINE HCL Microbiological assay of antibiotics

D-PANTHENOL Method A for determination of nitrogen

DRIED FERROUS

SULPHATE


Indicator

0.1M ceric amm. sulphate

ferroin sol.

DRIED HUMAN

ANTIHAEMOPHILIC

FRACTION

Biological assay of human antihaemophilic fraction

For sodium ions-

FP method B

λmax 589 nm

DYDROGESTERONE

TAB.

DYDROGESTERONE

UV visible

spectroscopy

λmax

A(1%, 1 cm)

286 nm

845

ECONAZOLE

NITRATE CREAM

GC

Packed column



to 100# coated with phenyl

methyl silicone

ECONAZOLE

NITRATE PESSARIES

ECONAZOLE

NITRATE


A

Solvent

End-point


Potentiometrically

EMETINE HCL INJ. Acid base titration Titrant

Indicator

0.1M NaOH

methyl red

EMETINE HCL Non aqueous titration

B

Solvent

Indicator


mercuric acetate

crystal violet.

ENALAPRIL

MALEATE TAB.

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

Acetonitrile +water + mixed

phosphate buffer pH 4.0

(68:30:2)

LC2 (SS 20 cm x 4.6 mm)

2.0 ml per minute

215 nm

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ENALAPRIL

MALEATE

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

phosphate buffer ph 6.8

Styrene-divinylbenzene

copolymer (5-10 mm)

SS (25 cm x 4.1 mm)

1.0 ml per minute

210 nm

EPHEDRINE HCL

ORAL SOL.

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

0.005M dioctyl sodium

sulphosuccinate in methanol +

water +

glacial acetic acid (65:35:1)

LC1 (10µm)

SS (20 cm x 4.6 mm)

1.5 ml per minute

263 nm

EPHEDRINE HCL

TAB.

EPHEDRINE HCL


B

Solvent

Indicator


mercuric acetate

crystal violet.

EPHEDRINE Acid base titration Titrant

Indicator

0.1M NaOH

methyl red

ERGOCALCIFEROL HPLC

Mb.phase

St.phase

Flow rate

Detection λ

hexane +1-pentanol (997:3)


2.0 ml per minute

254 nm

ERGOMETRINE

MALEATE INJ.

ERGOMETRINE

MALEATE TAB.

UV visible

spectroscopy

λmax 545 nm

ERGOMETRINE

MALEATE


A

Solvent

End-point


Potentiometrically

ERGOTAMINE

TARTRATE INJ.

ERGOTAMINE

TARTRATE TAB.

UV visible

spectroscopy

λmax 545 nm

ERGOTAMINE

TARTRATE


A

Solvent

End-point


Potentiometrically

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ERYTHROMYCIN

ESTOLATE TAB.

ERYTHROMYCIN

ESTOLATE

ERYTHROMYCIN

STEARATE TAB.

ERYTHROMYCIN

STEARATE

ERYTHROMYCIN

TAB.

ERYTHROMYCIN


ETHACRYNIC ACID

TAB.

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

Acetonitrile + glacial acetic acid

+ propyl hydroxybenzoate

(45:0.5:5)

LC1 (10 µl)

SS (20 cm x 4 mm)

2 ml per minute

270 nm

ETHACRYNIC ACID Acid base titration Titrant

End-point

0.1M NaOH

Potentiometrically

ETHAMBUTOL HCL

TAB.


B

Solvent

Indicator


1-naphtholbenzein

ETHAMBUTOL HCL Non aqueous titration

B

Solvent

Indicator


mercuric acetate

crystal violet.

ETHINYLOESTRADIO

L TAB.

Flourimetry Excitation λ

Emission λ

290 nm

320nm

ETHINYLOESTRADIO

L

Acid base titration Solvent

Titrant

End-point

Tetrahydrofuran + AgNO3

0.1M NaOH

Potentiometrically

ETHIONAMIDE TAB.

ETHIONAMIDE


A

Solvent

End-point


Potentiometrically

ETHOPROPAZINE

HCL TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

252 nm

845

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ETHOPROPAZINE

HCL


B

Solvent

Indicator


mercuric acetate

crystal violet

ETHOSUXIMIDE

CAPSULES


B

Solvent

Titrant

Indicator

End-point

dimethylformamide


azo violet

violet color

ETHOSUXIMIDE

SYRUP

GC

Packed column



to 100# coated

cyanopropylmethyl phenyl

methyl silicone

ETHOSUXIMIDE Non aqueous titration

B

Solvent

Titrant

Indicator

End-point

dimethylformamide

tetrabutylamm.hydroxide

thymolphthalein

blue color

ETHYL OLEATE Determination of esters

ETHYLENEDIAMINE

HYDRATE


Indicator

End point

1M HCl

bromophenol blue

yellow colour

ETHYLOESTRENOL

TAB.

ETHYLOESTRENOL

GC

Packed column

glass(1 m x 4 mm) packed with

silanised diatomaceous 80 to

100# coated with phenyl

methyl silicone

EUCALYPTUS OIL Determination of cineole

FENFLURAMINE HCL

TAB.

FENFLURAMINE HCL

GC

Packed column

glass(2.75 m x 4 mm)

packed with silanised

diatomaceous 80 to 100#

coated with polyethylene glycol

compound + potassium

hydroxide

FERROUS

FUMARATE TAB.

FERROUS

FUMARATE


Indicator


ferroin

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FERROUS

GLUCONATE TAB.

FERROUS

GLUCONATE

FERROUS SULPHATE

TAB.

FERROUS SULPHATE


Indicator


ferroin

FLUDROCORTISONE

ACETATE TAB.

FLUDROCORTISONE

ACETATE

Assay of steroids

FLUOCINOLONE

ACETONIDE CREAM

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

n-hexane + chloroform

+methanol (58:40:2) +

0.1 ml glacial acetic acid

LC3 (5 µm)

SS (20 cm x 5 mm)

1.8 ml per minute

243 nm

FLUOCINOLONE

ACETONIDE

Assay of steroids

FLUORESCEIN

SODIUM EYE DROPS

FLUORESCEIN

SODIUM


FLUOROURACIL INJ. UV visible

spectroscopy

λmax

A(1%, 1 cm)

266 nm

552

FLUOROURACIL Non aqueous titration

B

Solvent

Titrant

Indicator

End-point

dimethylformamide


thymol blue

blue color

FLUPHENAZINE

DECANOATE INJ.

FLUPHENAZINE

DECANOATE


B

Solvent

Indicator


crystal violet

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FLUPHENAZINE HCL

INJ.

FLUPHENAZINE HCL

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

258 nm

620

FLUPHENAZINE HCL Non aqueous titration

A

Solvent

End point

anhyd. formic acid + acetic

anhydride

potentiometrically

FLURBIPROFEN TAB. UV visible

spectroscopy

λmax

A(1%, 1 cm)

247 nm

802

FLURBIPROFEN Acid base titration Titrant

Indicator

0.1M NaOH


FOLIC ACID TAB. HPLC

Mb.phase

St.phase

Flow rate

Detection λ

0.05M KH2PO4 + Acetonitrile

(93:7) + 5M NaOH to pH 6.0

LC1 (10 µm)

SS (20 cm x 4.6 mm)

2 ml per minute

283 nm

FOLIC ACID UV visible

spectroscopy

λmax 550 nm

FORTIFIED

BENZATHINE

PENICILLIN INJ.

UV visible

spectroscopy

λmax 325 nm

FORTIFIED

PROCAINE

PENICILLIN INJ.


Indicator

1M NaOH

methyl red

FRAMYCETIN

SULPHATE


FRUSEMIDE INJ.

FRUSEMIDE TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

271 nm

580

FRUSEMIDE Acid base titration Solvent

Titrant

Indicator

End-point

dimethylformamide

0.1M NaOH

bromothymol blue

blue color

FURAZOLIDONE

ORAL SUSPENSION

UV visible

spectroscopy

λmax

A(1%, 1 cm)

367 nm

750

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FURAZOLIDONE TAB.

FURAZOLIDONE

UV visible

spectroscopy

λmax

A(1%, 1 cm)

367 nm

750

FUSIDIC ACID ORAL

SUSPENSION

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

methanol+ Acetonitrile+

glacial acetic acid (10:65:30)

LC1 (5 µm)

SS (20 cm x 4.6 mm)

1.5 ml per minute

235 nm

FUSIDIC ACID Acid base titration Titrant

Indicator

0.1M NaOH

phenolphthalein

GALLAMINE

TRIETHIODIDE INJ.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

225 nm

525

GALLAMINE

TRIETHIODIDE


A

Solvent

End-point

Acetone + mercuric

acetate

Potentiometrically

GENTAMICIN

SULPHATE EYE

DROPS

GENTAMICIN

SULPHATE INJ.

GENTAMICIN

SULPHATE

Microbiological Assay of antibiotics

GLIBENCLAMIDE

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

300 nm

63

GLIBENCLAMIDE

GLYCERIN


Indicator

0.1M NaOH


GLYCERYL

MONOSTEARATE

Iodimetric titration Titrant

Indicator

Sodium thiosulphate

Starch

GLYCERYL

TRINITRATE TAB.

UV visible

spectroscopy

λmax 405 nm

GLYCINE Non aqueous titration

B

Solvent

Indicator


crystal violet.

GRISEOFULVIN TAB. UV visible

spectroscopy

λmax 266 nm

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GRISEOFULVIN UV visible

spectroscopy

λmax

A(1%, 1 cm)

291 nm

686

GUAIPHENESIN Iodimetric titration Titrant

Indicator

0.05M iodine

starch

GUGGUL RESIN

GUGULIPID TAB.

GUGULIPID

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

Acetonitrile +water (65:35)

LC1(SS 25 cmx4.6 mm)

1.0 ml per minute

242 nm

HALOPERIDOL INJ.

HALOPERIDOL ORAL

SOL.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

245 nm

346

HALOPERIDOL TAB. HPLC

Mb.phase

St.phase

Flow rate

Detection λ

Acetonitrile + amm. Acetate

(45:55)

LC1 (5 µm)

SS (15 cm x 5 mm)

2 ml per minute

247 nm

HALOPERIDOL Non aqueous titration

B

Solvent

Indicator


1-naphtholbenzein

HEAVY MAGNESIUM

CARBONATE

HEAVY MAGNESIUM

OXIDE

Complexometric

titration

Titrant

Indicator

0.05M disod. Edetate


HEPARIN SODIUM

INJ.

HEPARIN SODIUM

Biological assay

HISTAMINE

PHOSPHATE INJ.


HISTAMINE

PHOSPHATE


A

Solvent

End-point


Potentiometrically

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HOMATROPINE

HYDROBROMIDE

EYE DROPS

GC

Packed column



to 100# coated phenyl methyl

silicone

HOMATROPINE

HYDROBROMIDE


B

Solvent

End-point


mercuric acetate

Potentiometrically

HUMAN ALBUMIN

HUMAN PLASMA

PROTEIN FRACTION

Method A for determination of nitrogen

For sodium ions-

FP method B

λmax 589 nm

For potassium

chloride -- FP OR

AAS method A

λmax 767 nm

HUMAN NORMAL

IMMUNOGLOBULIN

Method E for determination of nitrogen

HYALURONIDASE UV visible

spectroscopy

λmax 640 nm

HYDRALAZINE HCL

INJ.

HYDRALAZINE HCL

Potentiometric

titration

Titrant

End point


potentiometrically

HCL Acid base titration Titrant

Indicator

1M NaOH

methyl red

HYDROCHLOROTHIA

ZIDE TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

273 nm

520

HYDROCHLOROTHIA

ZIDE


A

Titrant

End-point


Potentiometrically

HYDROCORTISONE

ACETATE EYE

OINTMENT

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

butyl chloride + methanol

tetrahydrofuran + glacial acetic

acid (425:35:70:30)

LC1 (10 µm)

SS (30 cm x 3.9 mm)

1.0 ml per minute

254 nm

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HYDROCORTISONE

ACETATE INJ.

HYDROCORTISONE

ACETATE

Assay of steroids

HYDROCORTISONE

HEMISUCCINATE


Indicator

1M NaOH

methyl red

HYDROCORTISONE

SODIUM SUCCINATE

INJ.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

248 nm

449

HYDROCORTISONE Assay of steroids

HYDROGEN

PEROXIDE SOL. (100

VOL) & (20 VOL)

Redox titration

Titrant 0.02M potassium permanganate

HYDROXOCOBALAM

IN INJ.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

351 nm

195

HYDROXOCOBALAM

IN

UV visible

spectroscopy

λmax

A(1%, 1 cm)

351 nm

188-190

HYDROXYPROGESTE

RONE HEXANOATE

INJ.

UV visible

spectroscopy

λmax

380 nm

HYDROXYPROGESTE

RONE HEXANOATE

UV visible

spectroscopy

λmax

A(1%, 1 cm)

240 nm

395

HYOSCINE

BUTYLBROMIDE INJ.

HYOSCINE

BUTYLBROMIDE

TAB.

UV visible

spectroscopy

λmax

420 nm

HYOSCINE

BUTYLBROMIDE


B

Solvent

Indicator

anhyd. glacial acetic acid +

mercuric acetate

1-naphtholbenzein

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HYOSCINE

HYDROBROMIDE INJ.

HYOSCINE

HYDROBROMIDE

TAB.

GC

Packed column




silicone

HYOSCINE

HYDROBROMIDE


A

Solvent

End-point


dioxan

Potentiometrically

IBUPROFEN TAB.

IBUPROFEN


Indicator

1M NaOH

methyl red

IDOXURIDINE EYE

DROPS

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

Water + methanol (87:13)

LC1 (10 µm)

SS (30 cm x 4 mm)

1.7ml per minute

254 nm

IDOXURIDINE Non aqueous titration

A

Solvent

Titrant

End-point

dimethylformamide

tetrabutylamm hydroxide

Potentiometrically

IMIPRAMINE HCL

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

250 nm

264

IMIPRAMINE HCL Non aqueous titration

B

Solvent

Indicator

CHCl3 +mercuric acetate

Metanil yellow

INDOMETHACIN

CAPSULES

INDOMETHACIN

SUPPOSITORIES

UV visible

spectroscopy

λmax

A(1%, 1 cm)

320 nm

193

INDOMETHACIN Acid base titration Titrant

Indicator

1M NaOH

phenolphthalein

INSULIN INJ.

INSULIN ZINC

SUSPENSION

(AMORPHOUS)

INSULIN ZINC

SUSPENSION

(CRYSTALLINE)

INSULIN ZINC

SUSPENSION

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

0.1 M NaH2PO4 (pH 2 set with

phosphoric acid)

+ Acetonitrile (72.5:27.5)

LC1 (5 µm)

SS (25 cm x 4.6 mm)

1.0 ml per minute

214 nm

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INSULIN HPLC

Mb.phase

St.phase

Flow rate

Detection λ

0.1 M NaH2PO4 (pH 2 set with

phosphoric acid)

+ Acetonitrile (72.5:27.5)

LC1 (5 µm)

SS (25 cm x 4.6 mm)

1.0 ml per minute

214 nm

INTRAPERITONEAL

DIALYSIS FLUID

For sodium-

FP OR

AAS method A

λmax 589 nm

For calcium-

Complexometric

titration

Titrant

Indicator


calcon mixture

For magnesium

Complexometric

titration

Titrant

Indicator



For total chloride

Precipitation

titration

Titrant

End point

0.1M AgNO3

Potentiometrically

For dextrose -- Polarimetry

IODINE

IRON AND AMM.

CITRATE

Iodometric titration Titrant

Indicator

0.1 M

sodium thiosulphate

starch

IRON DEXTRAN INJ. For iron –redox

titration

Titrant

Indicator

ceric amm. sulphate

ferroin

For dextrans

UV visible

spectroscopy

λmax 625 nm

ISONIAZID TAB.

ISONIAZID

Bromometric titration

Titrant

Indicator

0.0167M potassium bromate

methyl red

ISOPHANE INSULIN

INJ.

As insulin

ISOPRENALINE HCL

INJ.

ISOPRENALINE

SULPHATE TAB.

UV visible

spectroscopy

λmax 540 nm

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ISOPRENALINE HCL Non aqueous titration

B

Solvent

Indicator


crystal violet.

ISOPRENALINE

SULPHATE


B

Solvent

Indicator


crystal violet.

ISOSORBIDE

DINITRATE TAB.

UV visible

spectroscopy

λmax 405 nm

ISOXSUPRINE HCL

INJ.

ISOXSUPRINE HCL

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

274 nm

73

ISOXSUPRINE HCL Non aqueous titration

B

Solvent

Indicator


1-naphtholbenzein

JAPANESE

ENCEPHALITIS

VACCINE, HUMAN

(MOUSE BRAIN)

Biological assay

KANAMYCIN ACID

SULPHATE

KANAMYCIN INJ.

KANAMYCIN

SULPHATE

Microbiological assay of antibiotics Method A or B

KETAMINE HCL INJ. UV visible

spectroscopy

λmax 269 nm

KETAMINE HCL Non aqueous titration

B

Solvent

Indicator

anh. Glacial acetic acid + anh.

Formic acid +

mercuric acetate

crystal violet

KETOCONAZOLE

TAB.

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

di-isopropylamine + amm.

acetate (7:3)

LC1 (10 µm)

SS (30 cm x39 mm)

3.0 ml per minute

225 nm

KETOCONAZOLE Non aqueous titration

A

Solvent

End-point


Potentiometrically

KETOPROFEN

CAPSULES

UV visible

spectroscopy

λmax

A(1%, 1 cm)

258 nm

662

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KETOPROFEN Acid base titration Titrant

Indicator

0.1M NaOH

phenolphthalein

LABETALOL HCL

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

302 nm

86

LABETALOL HCL Non aqueous titration

B

Solvent

Indicator


mercuric acetate

oracet blue B

LACTIC ACID Acid base back

titration

Titrant

Indicator

1M HCl

phenolphthalein

LANATOSIDE C TAB.

LANATOSIDE

UV visible

spectroscopy

λmax 484 nm

LEVAMISOLE HCL

TAB.


B

Solvent

Indicator

anh. Glacial acetic acid.

crystal violet.

LEVAMISOLE HCL Acid base titration Titrant

End point

0.1M NaOH

potentiometrically.

LEVODOPA AND

CARBIDOPA TAB.

HPLC Mb.phase

St.phase

Flow rate

Detection λ

NaH2PO4 + sodium 1-

decanesulfonate (950:1.3)

pH set 2.8 with phosphoiric acid

LC2 SS (30 cm x 3.9 mm)

2 ml per minute

280 nm

LEVODOPA

CAPSULES

LEVODOPA TAB.


B

Solvent

Indicator

anh. Glacial acetic acid + anhyd.

formic acid

oracet blue B.

LEVODOPA Non aqueous titration

A

Solvent

Indicator


dioxan

crystal violet.

LEVONORGESTREL

AND

ETHINYLOESTRADIO

L TAB.

HPLC

Mb.phase

St.phase

Retention time

Detection λ

Acetonitrile + water (55:45)

LC1 SS (25 cm x 4.6 mm)

10 minutes

241 , 281 , 305 nm

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LEVONORGESTREL UV visible

spectroscopy

λmax 241 nm

LIGHT Mg

CARBONATE

LIGHT MAGNESIUM

OXIDE

Complexometric

titration

Titrant

Indicator



LIGNOCAINE HCL

AND ADRENALINE

BITARTRATE INJ.

For lignocaine HCl


B

Solvent

Indicator


crystal violet

For adrenaline

UV visible

spectroscopy

λmax 540 nm

LIGNOCAINE HCL

AND DEXTROSE IN

Polarimetry

LIGNOCAINE HCL

GEL

Complexometric

titration

Titrant

Indicator

End point


sulphosuccinate

dimethyl yellow-oracet blue B.

green to pinkish grey

(chloroform)

LIGNOCAINE HCL

INJ.


B

Solvent

Indicator


crystal violet.

LIGNOCAINE HCL Non aqueous titration

B

Solvent

Indicator


mercuric acetate

crystal violet.

LINCOMYCIN HCL

CAPSULES

LINCOMYCIN HCL

GC

Packed column




silicone

LIQUID PARAFFIN

EMULSION


LIQUORICE TLC (250 nm) Coating

Mb.phase

silica gel GF254

ethanol + ethyl acetate

+ammonia

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LITHIUM

CARBONATE TAB.

LITHIUM

CARBONATE


Indicator

1M NaOH

methyl orange

LOMUSTINE

CAPSULES

UV visible

spectroscopy

λmax

A(1%, 1 cm)

230 nm

260

LOMUSTINE Redox titration Titrant

End point

0.05M AgNO3

potentiometrically

MAGALDRATE ORAL

SUSPENSION

MAGALDRATE TAB.

MAGALDRATE

Acid base back

titration

Titrant

End point

0.1M NaOH

Potentiometrically

MAGNESIUM

CHLORIDE

MAGNESIUM

HYDROXIDE

Complexometric

titration

Titrant

Indicator



MAGNESIUM

HYDROXIDE ORAL

SUSPENSION

Acid base back

titration

Titrant

Indicator

0.1M NaOH

methyl orange

MAGNESIUM

STEARATE

Complexometric

titration

Titrant

Indicator

Color change

0.1M zinc sulphate


blue to violet

MAGNESIUM

SULPHATE

As magnesium chloride

MAGNESIUM

TRISILICATE

For magnesium oxide - As magnesium chloride

For SiO2 - Gravimetric titration

MALT EXTRACT Method A determination of nitrogen

MANNITOL INJ.

MANNITOL


End point

0.05M iodine

yellow colour

MEBENDAZOLE TAB. UV visible

spectroscopy

λmax 234 nm

MEBENDAZOLE Non aqueous titration

A

Solvent

End-point


Formic acid

Potentiometrically

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MEBEVERINE HCL

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

263 nm

263

MEBEVERINE HCL Non aqueous titration

A

Solvent

End-point


mercuric acetate

Potentiometrically

MECLIZINE HCL TAB.

MECLIZINE HCL


B

Solvent

Indicator


mercuric acetate

quinaldine red

MEFENAMIC ACID

CAPSULES

MEFENAMIC ACID


Indicator

0.1M NaOH

phenol red

MEGESTROL

ACETATE TAB.

MEGESTROL

ACETATE

UV visible

spectroscopy

λmax

A(1%, 1 cm)

287 nm

630

MELPHALAN INJ.

MELPHALAN TAB.

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

(NH4)2CO3 + methanol + glacial

acetic acid

(200:180:2.7)

LC1 (10 µm)

SS (20 cm x 4.6 mm)

2.0 ml per minute

254 nm

MELPHALAN Redox titration Titrant

End-point

AgNO3

Potentiometrically

MENADIONE Redox titration Titrant

Indicator

ceric amm. sulphate

ferroin sol.

MENTHA OIL Acid base back

titration

Titrant

Indicator

1M HCl

phenolphthalein

MENTHOL GC

Packed column


with diatomaceous 125 to 180 #

impregnated with fatty acids

MEPHENTERMINE

SULPHATE INJ.


Indicator

1M NaOH

methyl red

MEPHENTERMINE

SULPHATE


Indicator

1M NaOH

methyl red

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MEPYRAMINE

MALEATE TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

316 nm

206

MEPYRAMINE

MALEATE


A

Solvent

End-point


Potentiometrically

MERCAPTOPURINE

TABLET

UV visible

spectroscopy

λmax

A(1%, 1 cm)

325 nm

1165

MERCAPTOPURINE Non aqueous titration

A

Solvent

Titrant

End-point

dimethylformamide


Potentiometrically

METFORMIN HCL

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

232 nm

798

METFORMIN HCL Non aqueous titration

A

Solvent

End-point

acetic anhydride + anh. Formic

acid

Potentiometrically

METHADONE HCL

INJ.

METHADONE HCL

TAB

UV visible

spectroscopy

λmax

A(1%, 1 cm)

350 nm

448

METHADONE HCL Non aqueous titration

B

Solvent

Indicator


mercuric acetate

crystal violet

METHDILAZINE HCL

TAB.

UV visible

spectroscopy

λmax 460 nm

METHDILAZINE HCL UV visible

spectroscopy

λmax 252 , 275 nm

METHOTREXATE INJ.

METHOTREXATE

TAB.

METHOTREXATE

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

phosphate buffer ph 6.0 +

Acetonitrile (92:8)

LC1 (5 µm)

SS (10 cm x 6 mm)

1.4 ml per minute

303 nm

METHOXAMINE HCL

INJ.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

290 nm

137

METHOXAMINE HCL Non aqueous titration

B

Solvent

Indicator

mercuric acetate sol. + acetic

anhydride

crystal violet

METHYL

SALICYLATE

Acid base back

titration

Titrant

Indicator

0.1M sulphuric acid

methyl red

METHYLCELLULOSE Determination of methoxyl groups

METHYLDOPA TAB. UV visible

spectroscopy

λmax

A(1%, 1 cm)

545 nm

89

METHYLDOPA Non aqueous titration

B

Solvent

Indicator


Formic acid

crystal violet.

METHYLERGOMETRI

NE MALEATE INJ.

METHYLERGOMETRI

NE MALEATE TAB.

METHYLERGOMETRI

NE MALEATE

UV visible

spectroscopy

λmax 550 nm

METHYLPARABEN Iodimetric titration Titrant

Indicator


starch

METHYLPREDNISOL

ONE ACETATE INJ.

METHYLPREDNISOL

ONE ACETATE

METHYLPREDNISOL

Assay of steroids

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METOPROLOL

TARTRATE


A

End-point Potentiometrically

METRONIDAZOLE

BENZOATE ORAL

SUSPENSION


A

Solvent

End-point

Acetone

Potentiometrically

METRONIDAZOLE

BENZOATE


A

Solvent

End-point

Acetone

Potentiometrically

METRONIDAZOLE

INJ.

UV visible

spectroscopy

λmax 277 nm

METRONIDAZOLE

TAB.


A

Solvent

Indicator

End-point

acetic anh.+acetone

brilliant green

yellowish-green

METRONIDAZOLE Non aqueous titration

A

Solvent

End-point


Potentiometrically

MIANSERIN HCL

TAB.

GC

Packed column

glass(1 m x 4 mm) packed with

silanised diatomaceous 80 to

100# coated phenyl methyl

silicone

MIANSERIN HCL Acid base titration Titrant

End point

0.1M NaOH

potentiometrically

MICONAZOLE

NITRATE CREAM

MICONAZOLE

NITRATE PESSARIES

GC

Packed column




silicone

MICONAZOLE

NITRATE


A

Solvent

End-point


Potentiometrically

MICROCRYSTALLIN

E CELLULOSE


Indicator

ferrous amm. sulphate

ferroin sulphate

MONOTHIOGLYCER

OL


Indicator

0.05M iodine

starch

MORPHINE

SULPHATE AND

ATROPINE

SULPHATE INJ.

Acid base back

titration

Titrant

Indicator

0.1M NaOH

methyl red

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MORPHINE

SULPHATE INJ.

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

sodium heptanesulphonate (0.73

g) in water + methanol + glacial

acetic acid (720:280:10)

LC1 (5 µm)

SS (40 cm x 4.6 mm)

1.5 ml per minute

280 nm

MORPHINE

SULPHATE


A

End-point Potentiometrically

NALIDIXIC ACID

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

334 nm

494

NALIDIXIC ACID Acid base titration Titrant

End point

0.1M ethanolic NaOH

potentiometrically

NALORPHINE HCL

INJ.

NALORPHINE HCL

UV visible

spectroscopy

λmax

285 nm

NANDROLONE

DECANOATE INJ.

UV visible

spectroscopy

λmax

380 nm

NANDROLONE

DECANOATE

UV visible

spectroscopy

λmax

A(1%, 1 cm)

239 nm

407

NANDROLONE

PHENYLPROPIONAT

E

UV visible

spectroscopy

λmax

A(1%, 1 cm)

240 nm

430

NEOMYCIN

SULPHATE EYE

DROPS

NEOMYCIN

SULPHATE EYE

OINTMENT

NEOMYCIN

SULPHATE

Microbiological assay of antibiotics , Method A.

NEOSTIGMINE

BROMIDE TAB.

Acid base back

titration

Titrant

Indicator

0.1M NaOH

methyl red

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NEOSTIGMINE

BROMIDE


B

Solvent

Indicator

anh. Glacial acetic acid + acetic

anhydride

crystal violet

NEOSTIGMINE

METHYLSULPHATE

INJ.

UV visible

spectroscopy

λmax 420 nm

NEOSTIGMINE

METHYLSULPHATE


Indicator

2 M HCl

methyl red-methylene blue

NICLOSAMIDE TAB.

NICLOSAMIDE


A

Solvent

Titrant

End-point

acetone and methanol


Potentiometrically

NICOTINAMIDE TAB. UV visible

spectroscopy

λmax

A(1%, 1 cm)

262 nm

241

NICOTINAMIDE Non aqueous titration

B

Solvent

Indicator

anh. Glacial acetic acid + acetic

anhydride

crystal violet

NICOTINIC ACID

TAB.

NICOTINIC ACID


Indicator

0.1M NaOH

phenol red

NICOUMALONE TAB. UV visible

spectroscopy

λmax

A(1%, 1 cm)

306 nm

521

NICOUMALONE Acid base titration Titrant

Indicator

0.1M NaOH

bromothymol blue

NIFEDIPINE

CAPSULES

NIFEDIPINE TAB

UV visible

spectroscopy

λmax 350 nm

NIFEDIPINE Redox titration Titrant

Indicator

End point


ferroin sol.

Pink

NIKETHAMIDE INJ. UV visible

spectroscopy

λmax

A(1%, 1 cm)

263 nm

282

NIKETHAMIDE Non aqueous titration

A

Solvent

End-point


acetic anhydride

Potentiometrically

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NITRAZEPAM TAB. UV visible

spectroscopy

λmax

A(1%, 1 cm)

280 nm

910

NITRAZEPAM Non aqueous titration

A

Solvent

End-point

acetic anhydride

Potentiometrically

NITROFURANTOIN

TAB.

NITROFURANTOIN

UV visible

spectroscopy

λmax

A(1%, 1 cm)

367 nm

765

NITROFURAZONE UV visible

spectroscopy

λmax

A(1%, 1 cm)

375 nm

822

NITROUS OXIDE Assay of nitrous oxide

NORADRENALINE

BITARTRATE INJ.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

279 nm

80

NORADRENALINE

BITARTRATE


B

Solvent

Indicator

anh. Glacial acetic acid.

crystal violet

NORETHISTERONE

TAB.

Acid base titration Solvent

Titrant

End-point

tetrahydrofuran + AgNO3

0.1M NaOH

Potentiometrically

NORETHISTERONE Acid base titration Solvent

Titrant

Indicator

tetrahydrofuran + AgNO3

0.1M NaOH

bromocresol green

NORFLOXACIN TAB. HPLC

Mb.phase

St.phase

Flow rate

Detection λ

Phosphoric acid + acetonitrile

(85:15)

LC1 SS (30 cm x 3.9 mm)

2 ml per minute

275 nm

NORFLOXACIN Non aqueous titration

A

Solvent

End-point


Potentiometrically

NORGESTREL AND

ETHINYLOESTRADIO

L TAB.

HPLC

Mb.phase

St.phase

Retention time

Detection λ

Acetonitrile + water (55:45)

LC1 SS (25 cm x 4.6 mm)

10 minutes

241 , 281 , 305 nm

NORGESTREL UV visible

spectroscopy

λmax 241 nm

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NORTRIPTYLINE

HCL TAB.

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

0.56 % w/v sodium

Hexanesulphonate sol in

+Acetonitrile+glacial acetic acid

(1:1) pH set 4.5 with Glacial

acetic acid

LC1 (10 µm)

SS (20 cm x 4.6 mm)

2 ml per minute

239 nm

NORTRIPTYLINE

HCL


A

Solvent

End-point


mercuric acetate

Potentiometrically

NOSCAPINE

LINCTUS

UV visible

spectroscopy

λmax

A(1%, 1 cm)

310 nm

90.7

NOSCAPINE Non aqueous titration

A

Solvent

End-point


Potentiometrically

NOVOBIOCIN

SODIUM


NYSTATIN

OINTMENT

NYSTATIN

PESSARIES

NYSTATIN TAB.

NYSTATIN

OESTRADIOL

BENZOATE INJ.

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

cyclohexane + dioxin (9:1)

LC3 (10 µm)

SS (30 cm x 4 mm)

2 ml per minute

254 nm

OESTRADIOL

BENZOATE

UV visible

spectroscopy

λmax

A(1%, 1 cm)

231 nm

500

OMEPRAZOLE

CAPSULES

OMEPRAZOLE

HPLC

Mb.phase

St.phaseFlow

rate

Detection λ

phosphate buffer pH 7.4 +

Acetonitrile (65:35)

LC1 SS (30 cm x 3.9 mm)

1.0 ml per minute

302 nm

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OPIUM POWDER

OPIUM

HPLC

Mb.phase

sationaryphase

Flow rate

Detection λ

sodium heptanesulphonate (1 g)

in water (420 ml) pH 3.2 with

phosphoric acid

LC2 (5 µm)

SS (25 cm x 4.6 mm)

1.5 ml per minute

280 nm

ORAL

REHYDRATION

SALTS

For total sodium

AAS method A

OR FP method B

λmax 589 nm

For potassium

AAS method A

OR FP method B

λmax 767 nm

For total chloride

Precipitation titration

Titrant

Indicator

0.1M AgNO3

potassium chromate

For citrate


B

Solvent

Indicator


1-naphtholbenzein

For dextrose – Polarimetry

ORMELOXIFEN HCL

TAB.

ORMELOXIFEN HCL

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

0.04 % w/v Tetramethyl Amm.

hydroxide in Acetonitrile +

water (80:20) pH set 7.6 with

phosphoric acid

LC1 SS (25 cm x 4.6 mm)

1.5 ml per minute

280 nm

OXPRENOLOL HCL

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

273 nm

74.5

OXPRENOLOL HCL Non aqueous titration

A

Solvent

End-point


mercuric acetate

Potentiometrically

OXYGEN Assay of oxygen

OXYPHENBUTAZON

E TAB.


Indicator

0.1M NaOH

bromothymol blue

gnu.i

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.in

OXYPHENBUTAZON

E


Indicator

0.1M NaOH

bromothymol blue

OXYTETRACYCLINE

HCL CAPSULES

Microbiological assay of antibiotics, Method A or B

OXYTETRACYCLINE

HCL EYE OINTMENT

OXYTETRACYCLINE

HCL INJ.

OXYTETRACYCLINE

HCL

OXYTETRACYCLINE

INJ.

OXYTETRACYCLINE

OXYTOCIN INJ. Biological assay of oxytocin

OXYTOCIN

PANCREATIN For protease action-

Acid base

titration

Titrant

Indicator

0.1M NaOH

phenolphthalein

For lipase action-

Acid base titration

Titrant

Indicator

0.1M NaOH

bromocresol purple

For amylase action-

Biochemical

method

PAPAIN Acid base titration Titrant

Indicator

0.1M NaOH

phenolphthalein

gnu.i

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PARACETAMOL

SYRUP

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

0.01M sodium butanesulphonate

in water + methanol + formic

acid

(85:15:0.4)

LC1 (10 µm)

SS (20 cm x 4.6 mm)

2 ml per minute

243 nm

PARACETAMOL TAB. UV visible

spectroscopy

λmax

A(1%, 1 cm)

257 nm

715

PARACETAMOL Redox titration Titrant

Indicator


ferroin sol.

PECTIN Acid base titration Titrant

Indicator

0.1M NaOH

phenolphthalein

PENICILLAMINE

TAB.


Indicator


dithizone in ethanol (95%)

PENICILLAMINE Non aqueous titration

A

Solvent

End-point


Potentiometrically

PENTAERYTHRITOL

TETRANITRATE TAB.

UV visible

spectroscopy

λmax 409 nm

PENTAMIDINE

ISETHIONATE INJ.

PENTAMIDINE

ISETHIONATE


A

Solvent

Titrant

End-point

dimethylformamide

tetrabutylamm. hydroxide

Potentiometrically

PENTAZOCINE HCL

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

278 nm

61.2

PENTAZOCINE HCL Non aqueous titration

B

Solvent

Indicator


crystal violet.

PENTAZOCINE

LACTATE INJ.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

278 nm

69

PENTAZOCINE

LACTATE

PENTAZOCINE


B

Solvent

Indicator


crystal violet

gnu.i

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PENTOBARBITONE

SODIUM TAB.


PENTOBARBITONE

SODIUM


Indicator

End point

0.1M ethanolic NaOH

thymolphthalein

pure blue colour

PEPPERMINT OIL For esters – Acid

Base titration

Titrant

Indicator

0.5M HCl

phenolphthalein

For free alcohols–

Acid

Base titration

Titrant

Indicator

0.5M NaOH

phenolphthalein

For ketones –

Acid

Base titration

Titrant

Indicator

End point

0.5M ethanolic KOH

methyl orange

orange-yellow colour

PEPSIN Determination of ability to digest coagulated egg albumin

PETHIDINE HCL INJ.

PETHIDINE HCL TAB.


B

Solvent

Indicator


1-naphtholbenzein

PETHIDINE HCL Non aqueous titration

B

Solvent

Indicator


mercuric acetate

crystal violet

PHENFORMIN HCL

TAB.

UV visible

spectroscopy

λmax 520 nm

PHENFORMIN HCL As pethidine HCl

PHENINDAMINE

TARTRATE TAB.

PHENINDAMINE

TARTRATE


B

Solvent

Indicator


oracet blue B.

PHENINDIONE TAB. UV visible

spectroscopy

λmax

A(1%, 1 cm)

278 nm

1310

PHENINDIONE Iodometric titration Titrant

Indicator


starch

PHENIRAMINE

MALEATE INJ.

PHENIRAMINE

MALEATE TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

265 nm

210

gnu.i

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PHENIRAMINE

MALEATE


B

Titrant

Indicator


1-naphtholbenzein

PHENOBARBITONE

SODIUM INJ.


End point

0.1M AgNO3

Potentiometrically

PHENOBARBITONE

SODIUM TAB.


PHENOBARBITONE

SODIUM

Potentiometric

titration

Titrant 0.1M ethanolic NaOH

PHENOBARBITONE

TAB.


PHENOBARBITONE Acid base titration Titrant

Indicator

0.1M ethanolic NaOH

thymolphthalein

PHENOL Iodimetric titration Titrant

Indicator


starch

PHENOLPHTHALEIN UV visible

spectroscopy

λmax

A(1%, 1 cm)

555 nm

1055

PHENOXYMETHYLP

ENICILLIN

POTASSIUM TAB.

UV visible

spectroscopy

λmax

325 nm

PHENOXYMETHYLP

ENICILLIN

POTASSIUM

Potentiometric

titration

Titrant

End point


potentiometrically

PHENTOLAMINE

MESYLATE INJ.


PHENTOLAMINE

MESYLATE


B

Solvent

Titrant

End-point

anhyd. 2-propanol


Potentiometrically

PHENYLBUTAZONE

TAB.

UV visible

spectroscopy

λmax

264 nm

PHENYLBUTAZONE Acid base titration Titrant

Indicator

0.1M NaOH

bromothymol blue

PHENYLEPHRINE

HCL INJ.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

273 nm

90

PHENYLEPHRINE

HCL


End-point

0.1M ethanolic NaOH

Potentiometrically

gnu.i

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PHENYLMERCURIC

ACETATE


Indicator

Amm. thiocyanate

KMnO4

PHENYLMERCURIC

NITRATE


End point

Amm. thiocyanate


PHENYTOIN SODIUM

INJ.

PHENYTOIN SODIUM

TAB.


B

Titrant

Solvent

Indicator


anhyd. pyridine

thymol blue

PHENYTOIN SODIUM Potentiometric

titration

Titrant 0.1M NaOH

PHOLCODINE

LINCTUS


B

Solvent

Indicator

ammonia

quinaldine red

PHOLCODINE Non aqueous titration

A

Solvent

Indicator


Potentiometrically

PHOSPHORIC ACID Acid base titration Titrant

Indicator

0.1M NaOH

phenolphthalein

PHTHALYLSULPHAT

HIAZOLE TAB.

Nitrite titration

Solvent

Titrant

dil. HCl

0.1M sodium nitrite

PHTHALYLSULPHAT

HIAZOLE


B

Solvent

Titrant

Indicator

dimethylformamide


thymolphthalein

PHYSOSTIGMINE

SALICYLATE INJ.


A

Solvent

End-point


Potentiometrically

PHYSOSTIGMINE

SALICYLATE

PILOCARPINE

NITRATE

As above

PIPERAZINE

ADIPATE TAB.


PIPERAZINE

ADIPATE


B

Solvent

Indicator


1-naphtholbenzein

PIPERAZINE

CITRATE SYRUP


PIPERAZINE

CITRATE


B

Solvent

Indicator


1-naphtholbenzein

PIPERAZINE

HYDRATE


B

Solvent

Indicator


1-naphtholbenzein

gnu.i

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PIPERAZINE

PHOSPHATE TAB.

PIPERAZINE

PHOSPHATE


PIROXICAM

CAPSULES

PIROXICAM

HPLC

Mb.phase

St.phaseFlow

rate

Detection λ

Buffer (7.72 g of anhyd. citric

acid + 5.35 g of sodium

phosphate in 1000 ml) +

methanol (55:45)

LC1 SS (25 cm x 4.6 mm)

1.2 ml per minute

254 nm

PLAGUE VACCINE Biological assay of plague vaccine

POTASSIUM

CHLORIDE

Precipitation

titration

Titrant

Indicator

0.1M AgNO3

potassium chromate

POTASSIUM

CITRATE


B

Solvent

Indicator


1-naphtholbenzein

POTASSIUM IODIDE Redox titration Titrant

End point


removal of pink color

(chloroform layer)

POTASSIUM

PERMANGANATE

POVIDONE-IODINE

SOL.

POVIDONE-IODINE


Indicator


starch

PRALIDOXIME

CHLORIDE INJ.

PRALIDOXIME

CHLORIDE

UV visible

spectroscopy

λmax 332 nm

PRAZOSIN HCL TAB. HPLC

Mb.phase

St.phase

Flow rate

Detection λ

0.01 % w/v diethylamine in

methanol + glacial acetic acid +

water

(96:2:2)

LC3 (5 µm)

SS (20 cm x 4 mm)

1.0 ml per minute

254 nm

gnu.i

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PRAZOSIN HCL Non aqueous titration

A

Solvent

End- point

acetic anhydride + anh. Formic

acid

Potentiometrically

PREDNISOLONE

TAB.

PREDNISOLONE

PREDNISONE TAB.

PREDNISONE

Assay of steroids

PREPARED STORAX Acid base titration Titrant

Indicator

0.1M NaOH

phenol red

PRIMAQUINE

PHOSPHATE TAB.

PRIMAQUINE

PHOSPHATE


A

Solvent

End-point


Potentiometrically

PROBENECID Acid base titration Titrant

Indicator

0.1M NaOH

bromothymol blue

PROCAINAMIDE HCL

INJ.

PROCAINAMIDE HCL

TAB.

PROCAINAMIDE HCL

Nitrite titration Titrant 0.1M sodium nitrite

PROCAINE HCL AND

ADRENALINE

BITARTRATE

For procaine HCl

Acid base titration

Titrant

Indicator

0.1M NaOH


For adrenaline – as in adrenaline

PROCAINE HCL Nitrite titration Titrant 0.1M sodium nitrite

PROCAINE

PENICILLIN

For penicillins

Potentiometric

titration

Titrant

End point


Potentiometrically

For procaine

UV visible

spectroscopy

λmax

A(1%, 1 cm)

290 nm

777

PROCHLORPERAZIN

E MALEATE TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

258 nm

620

gnu.i

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PROCHLORPERAZIN

E MALEATE


A

Solvent

End-point


Potentiometrically

PROCHLORPERAZIN

E MESYLATE INJ.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

258 nm

635

PROCHLORPERAZIN

E MESYLATE


B

Solvent

Indicator


1-naphtholbenzein

PROGUANIL HCL

TAB.

UV visible

spectroscopy

λmax 480 nm

PROGUANIL HCL Non aqueous titration

A

Solvent

End-point


mercuric acetate

Potentiometrically

PROMETHAZINE HCL

INJ.

PROMETHAZINE HCL

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

249 nm

910

PROMETHAZINE HCL

SYRUP

UV visible

spectroscopy

λmax 336 nm

PROMETHAZINE HCL Acid base titration Titrant

End-point

0.1M NaOH

Potentiometrically

PROMETHAZINE

THEOCLATE TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

255 nm

755

PROMETHAZINE

THEOCLATE


B

Solvent

Indicator

acetone

methyl orange

PROPANTHELINE

BROMIDE TAB.

PROPANTHELINE

BROMIDE


B

Solvent

Indicator


mercuric acetate

crystal violet

PROPRANOLOL HCL

INJ.

PROPRANOLOL HCL

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

290 nm

206

PROPRANOLOL HCL Acid base titration Titrant

End-point

1M NaOH

Potentiometrically

PROPYLPARABEN Iodimetric titration Titrant

Indicator


starch

gnu.i

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et.ac

.in

PROPYLTHIOURACIL

TAB.


Indicator

End point


1,5-diphenylcarbazone

pinkish violet colour

PROPYLTHIOURACIL Potentiometric

titration

Titrant

End-point

AgNO3

Potentiometrically

PROPYPHENAZONE Non aqueous titration

A

Solvent

End-point


Potentiometrically

PROTAMINE

SULPHATE INJ.

Absorbance in visible range

PROTAMINE

SULPHATE

PROTAMINE ZINC

INSULIN INJ.

HPLC

As in insulin inj.

PSEUDOEPHEDRINE

HCL SYRUP

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

ethanol + amm. Acetate (85:15)

LC1 SS (25 cm x 4.2 mm)

1.5 ml per minute

254 nm

PSEUDOEPHEDRINE

HCL TAB.

HPLC

Mb.phase

St.phase

Flow rate

Detection λ


sulphosuccinate in methanol +

water + glacial acetic acid

(65:35:1)

methanol + LC1 (10µm)

SS (20 cm x 4.6 mm)

1.5 ml per minute

258 nm

PSEUDOEPHEDRINE

HCL


A

Solvent

Indicator


mercuric acetate

crystal violet

PSORALEN UV visible

spectroscopy

λmax 247 nm

PYRAZINAMIDE

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

268 nm

650

PYRAZINAMIDE Acid base back

titration

Titrant

Indicator

0.1M NaOH

methyl red

PYRIDOXINE HCL

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

290 nm

420

gnu.i

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PYRIDOXINE HCL Non aqueous titration

B

Solvent

Indicator


mercuric acetate

crystal violet

PYRIMETHAMINE

AND

SULPHADOXINE

TAB.

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

Acetonitrile+ glacial acetic acid

(4:1)

LC1 SS (30 cm x 4 mm

2 ml per minute

254 nm

PYRIMETHAMINE Non aqueous titration

A

Solvent

End-point


acetic anhydride

Potentiometrically

QUINALBARBITONE

SODIUM TAB.


QUINALBARBITONE

SODIUM


Indicator

0.1M ethanolic NaOH

thymolphthalein

QUINIDINE

SULPHATE TAB.


B

Solvent

Indicator


crystal violet

QUINIDINE

SULPHATE

QUININE

BISULPHATE TAB


A

Solvent

End-point


acetic anhydride

Potentiometrically

QUININE

BISULPHATE

As quinidine sulphate tab

QUININE DIHCL INJ.

QUININE DIHCL Non aqueous titration

B

Solvent

Indicator


+mercuric acetate

crystal violet

QUININE SULPHATE

TAB.

QUININE SULPHATE


A

Solvent

End-point

acetic anhydride

Potentiometrically

QUINIODOCHLOR

TAB.

UV visible

spectroscopy

λmax 650 nm

gnu.i

nflibn

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.in

QUINIODOCHLOR Non aqueous titration

A

Titrant

Solvent

End-point


anhyd. pyridine

Potentiometrically

RABIES ANTISERUM Biological assay of rabies antiserum

RABIES VACCINE,

HUMAN (CELL

CULTURE)

Biological assay of rabies vaccine

RABIES VACCINE,

HUMAN (NEURAL

TISSUE)

Biological assay of rabies vaccine

RANITIDINE HCL INJ.

RANITIDINE HCL

TAB.

RANITIDINE HCL

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

Amm. Acetate+ methanol

(15:85)

LC1 SS (25 cm x 4 mm)

2 ml per minute

322 nm

RESERPINE INJ.

RESERPINE TAB.

UV visible

spectroscopy

λmax 344 nm

RESERPINE For total alkaloids – as quinine sulphate

For reserpine - UV

visible spectroscopy

λmax 388 nm

RIBOFLAVINE

SODIUM PHOSPHATE

RIBOFLAVINE TAB

RIBOFLAVINE

UV visible

spectroscopy

λmax

A(1%, 1 cm)

444 nm

328

RIFAMPICIN

CAPSULES

RIFAMPICIN

UV visible

spectroscopy

λmax

A(1%, 1 cm)

475 nm

187

SACCHARIN SODIUM Non aqueous titration

A

Solvent

End-point


Potentiometrically

SACCHARIN Acid base titration Titrant

Indicator

0.1M NaOH

phenolphthalein

SALBUTAMOL

SULPHATE INJ.

UV visible

spectroscopy

λmax 605 nm

gnu.i

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SALBUTAMOL

SULPHATE SYRUP

SALBUTAMOL

SULPHATE TAB.

HPLC Mb.phase

St.phase

Flow rate

Detection λ

Water + 0.05M amm. acetate +

propanol (65:30:5) pH set 4.5

with glacial acetic acid

spherical particles of silica

(5µm) SS (20 cm x 5 mm)

2 ml per minute

276 nm

SALBUTAMOL

SULPHATE

SALBUTAMOL


A

Solvent

End-point


Formic acid

Potentiometrically

SALICYLIC ACID Acid base titration Titrant

Indicator

0.1M NaOH

phenol red

SELF-EMULSIFYING

GLYCERYLMONOST

EARATE

For free glycerin,

monoglycerides

Iodimetric titration

Titrant

Indicator


starch

For soap – acid base

titration

Titrant

Indicator

0.1M HCl

bromophenol blue

SENNA FRUIT

SENNA LEAF

UV visible

spectroscopy

λmax

A(1%, 1 cm)

515 nm

240

SILVER NITRATE Redox titration Titrant

End point

Amm. thiocyanate

Reddish yellow colour

SODIUM ACETATE Non aqueous titration

B

Solvent

Indicator


acetic anhydride

1-naphtholbenzein

SODIUM

AMINOSALICYLATE

GRANULES

Nitrite titration Solvent

Titrant

HCl + potassium bromide

0.1M sodium nitrite

SODIUM

AMINOSALICYLATE

TAB.

SODIUM

AMINOSALICYLATE

SODIUM

ASCORBATE


Indicator

0.05M iodine

starch

gnu.i

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SODIUM

AUROTHIOMALATE

INJ.


SODIUM

AUROTHIOMALATE

For Au & Na - Gravimetric titration

SODIUM BENZOATE Non aqueous titration

B

Solvent

Indicator


1-naphtholbenzein

SODIUM

BICARBONATE


Indicator

1M HCl

methyl orange

SODIUM

CARBOXYMETHYL-

CELLULOSE


A

Solvent

End-point


Potentiometrically

SODIUM CHLORIDE

AND DEXTROSE INJ.

Dextrose – Polarimetry

For sodium chloride

–precipitation

titration

Titrant

Indicator

0.1M AgNO3

potassium chromate

SODIUM CHLORIDE

HYPERTONIC INJ.

SODIUM CHLORIDE

INJ.

SODIUM CHLORIDE

redox titration Titrant

Indicator

End point

0.1M Amm. thiocyanate



SODIUM CITRATE Non aqueous titration

A

Solvent

Indicator


1-naphtholbenzein

SODIUM

CROMOGLYCATE

CARTRIDGES

UV visible

spectroscopy

λmax

A(1%, 1 cm)

327 nm

164

SODIUM

CROMOGLYCATE


A

Solvent

End-point

ethane-1,2-diol + 2-propanol +

dioxan

Potentiometrically

SODIUM

DIATRIZOATE INJ.

SODIUM

DIATRIZOATE


End point


dark brown colour

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SODIUM

DIHYDROGEN

PHOSPHATE

DIHYDRATE


End-point

1M NaOH

Potentiometrically

SODIUM FLUORIDE Non aqueous titration

B

Solvent

Indicator


acetic anhydride

crystal violet

SODIUM

FORMALDEHYDE

SULPHOXYLATE


Indicator

0.05M iodine

starch

SODIUM FUSIDATE

CAPSULES

HPLC Mb.phase

St.phase

Flow rate

Detection λ

Acetonitrile + glacial acetic acid

+ methanol

(60:30:10)

LC3 (5 µm)

SS (20 cm x 4.6 mm)

1.5 ml per minute

235 nm

SODIUM FUSIDATE Acid base titration Titrant

End-point

0.1M HCl

Potentiometrically

SODIUM

HYDROXIDE


Indicator

1M HCl

phenolphthalein

SODIUM LACTATE

INJ.

Acid base back

titration

Titrant

Indicator

1M HCl

methyl orange

SODIUM LAURYL

SULPHATE


Indicator

End point

benzethonium chloride

dimethyl yellow-oracet blue B

chloroform layer gains green

colour.

SODIUM

METABISULPHITE

SODIUM

METHYLPARABEN


Indicator

sodium thiosulphate

starch

SODIUM PHOSPHATE Acid base titration Titrant

End-point

NaOH

Potentiometrically

gnu.i

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SODIUM

PROPYLPARABEN

As sodium metabisulphite

SODIUM

SALICYLATE


A

Solvent

End-point


Potentiometrically

SODIUM STARCH

GLYCOLLATE


Indicator

0.1M NaOH

phenolphthalein

SODIUM

STIBOGLUCONATE

INJ.


End point

0.1M Ferric amm. sulphate

potentiometrically

SODIUM

STIBOGLUCONATE

SODIUM

THIOSULPHATE INJ.

SODIUM

THIOSULPHATE

SODIUM

VALPROATE ORAL

SOL.

SODIUM

VALPROATE TAB


Indicator

0.1M NaOH

phenolphthalein

SODIUM

VALPROATE


A

Solvent

End-point


Potentiometrically

SOLUBLE ASPIRIN

TAB.


Indicator


starch

SORBIC ACID As sodium valproate oral sol.

SORBITOL SOL. (70

PER CENT) (NON-

CRYSTALLISING)

For solid matter -Gravimetric titration

For polyols – as sorbitol

SORBITOL Iodimetric titration Titrant

Indicator

0.05M iodine

starch

SPIRONOLACTONE

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

238 nm

470

SPIRONOLACTONE

gnu.i

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STEARIC ACID GC Packed column



to 120# coated diethylene

glycol succinate polyester

STERILE TRISODIUM

EDETATE

CONCENTRATE FOR

INJ.

Complexometric

titration

Titrant

Indicator

0.05M magnesium sulphate


STILBOESTROL TAB. UV visible

spectroscopy

λmax 418 nm

STILBOESTROL

STREPTOKINASE INJ. Biological assay of streptokinase

STREPTOKINASE

STREPTOMYCIN

SULPHATE INJ.

Microbiological assay of antibiotics, method A or B

STREPTOMYCIN

SULPHATE TAB.

STREPTOMYCIN

SULPHATE

SUCCINYLCHOLINE

CHLORIDE INJ.

Acid base back

titration

Titrant

Indicator

0.1M HCl

bromo thymol blue

SUCCINYLCHOLINE

CHLORIDE


A

Solvent

Indicator


acetic anhydride+ mercuric

acetate sol.

crystal violet.

SUCCINYLSULPHAT

HIAZOLE TAB.


Titrant

HCl

0.1M sodium nitrite

SUCCINYLSULPHAT

HIAZOLE

SULPHACETAMIDE

SODIUM EYE DROPS

SULPHACETAMIDE

SODIUM EYE

OINTMENT

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SULPHACETAMIDE

SODIUM


Titrant

HCl

0.1M sodium nitrite

SULPHADIAZINE

TAB.

SULPHADIAZINE

SULPHADIMETHOXI

NE TAB.

SULPHADIMETHOXI

NE

SULPHADIMIDINE

SODIUM INJ.

SULPHADIMIDINE

SODIUM

SULPHADIMIDINE

TAB.

SULPHADIMIDINE

SULPHADOXINE

SULPHAFURAZOLE

TAB.


B

Solvent

Titrant

Indicator

dimethylformamide


thymol blue in methanol SULPHAFURAZOLE

SULPHALENE Nitrite titration Solvent

Titrant

HCl

0.1M sodium nitrite SULPHAMETHIZOLE

SULPHAMETHOXAZ

OLE

SULPHAPHENAZOLE

TAB.

SULPHAPHENAZOLE

TAB.

SULPHOBROMOPHT

HALEIN SODIUM INJ.

UV visible

spectroscopy

λmax 580 nm

SULPHOBROMOPHT

HALEIN SODIUM

For sulphur –Gravimetric titration

For bromine-

Redox titration

Titrant

Indicator

Amm. thiocyanate


TAMOXIFEN

CITRATE TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

275 nm

325

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TAMOXIFEN

CITRATE


B

Solvent

Indicator


1-naphtholbenzein

TARTARIC ACID Acid base titration Titrant

Indicator

0.1M NaOH

phenolphthalein

TERBUTALINE

SULPHATE INJ.

UV visible

spectroscopy

λmax 550 nm

TERBUTALINE

SULPHATE TAB.

TERBUTALINE

SULPHATE


A

Solvent

End-point


Potentiometrically

TESTOSTERONE

PROPIONATE INJ.

UV visible

spectroscopy

λmax 380 nm

TESTOSTERONE

PROPIONATE

UV visible

spectroscopy

λmax

A(1%, 1 cm)

241 nm

490

TETANUS

ANTITOXIN

Biological assay of tetanus antitoxin

TETANUS VACCINE

(ADSORBED)

Biological assay of adsorbed tetanus vaccine

TETRACYCLINE HCL

CAPSULES

HPLC Mb.phase

St.phase

Flow rate

Detection λ

Amm. Oxalate

+Dimethylformamide

+dibasic amm. phosphate

(680:270:50) pH set 7.6-7.7

with 3M ammonia or 3M

phosphoric acid

LC2 (5 to 10 µm)

SS (20 cm x 4.6 mm)

2 ml per minute

280 nm

TETRACYCLINE HCL

EYE OINTMENT

TETRACYCLINE HCL Microbiological assay of antibiotics, Method A or B

TETRACYCLINE

THEOPHYLLINE INJ. For theophylline

UV visible

spectroscopy

λmax 274 nm

For dextrose – Polarimetry

THEOPHYLLINE Acid base titration Titrant

Indicator

0.1M NaOH

bromothymol

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THIABENDAZOLE

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

302 nm

1230

THIABENDAZOLE Non aqueous titration

A

Solvent

End-point


Potentiometrically

THIACETAZONE AND

ISONIAZID TAB.

For thiacetazone

UV visible

spectroscopy

λmax 328 nm

For isoniazid-

bromometry

Titrant

End-point

Potassium bromate

potentiometrically

THIACETAZONE Gravimetric titration

THIAMINE HCL INJ. HPLC

Mb.phase

St.phase

Flow rate

Column

1g sodium heptanesulphonate in

Methanol + triethylamine

+water (180:10:810)

LC1 (5 µm)

SS (10 cm x 4.6 mm)

2 ml per minute

244 nm

THIAMINE HCL TAB.

THIAMINE HCL Non aqueous titration

A

Solvent

End-point

anhyd.formic acid + anhyd.

Glacial acetic acid + acetic

anhydride

Potentiometrically

THIAMINE

MONONITRATE

THIOMERSAL Redox titration Titrant

Indicator

Amm. thiocyanate


THIOPENTONE

SODIUM INJ.

THIOPENTONE

SODIUM

For thiopentone -

Non aqueous

titration

Titrant

Indicator

lithium methoxide

thymol blue

For sodium -

Acid base titration

Titrant

Indicator

End point

0.05M sulphuric acid

methyl red sol.

yellow → red

THIOTEPA INJ. HPLC Mb.phase

St.phase

Flow rate

Detection λ

Water + Acetonitrile (7:3)

LC1 (5 µm)

SS (20 cm x 4.6 mm)

1.0 ml per minute

215 nm

THIOTEPA Acid base titration Titrant

Indicator

1M HCl

methyl orange

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THYMOL Acid base titration Titrant

Indicator

0.05M bromine

methyl orange

THYROXINE SODIUM

TAB.

HPLC Mb.phase

St.phase

Flow rate

Detection λ

Water + Acetonitrile (65:35)

porous silica (5 to 10 µm)

SS (20 cm x 4.6 mm)

1.0 ml per minute

225 nm

THYROXINE SODIUM Iodometric titration Titrant

Indicator


starch

TIMOLOL MALEATE

EYE DROPS

UV visible

spectroscopy

λmax 295 nm

TIMOLOL MALEATE

TAB.

TIMOLOL MALEATE Non aqueous titration

A

Solvent

End-point


Potentiometrically

TINIDAZOLE TAB. UV visible

spectroscopy

λmax

A(1%, 1 cm)

310 nm

356

TINIDAZOLE Non aqueous titration

B

Solvent

Indicator

acetic anhydride

nile blue A sol.

TITANIUM DIOXIDE Redox titration Titrant

Indicator


ferroin

TOBRAMYCIN INJ. Microbiological assay of antibiotics, Method B

TOBRAMYCIN

TOCOPHERYL

ACETATE

As titanium dioxide

TOLBUTAMIDE TAB. Acid base titration Titrant

Indicator

0.1M NaOH

phenolphthalein TOLBUTAMIDE

TOLU BALSAM Acid base titration Titrant

Indicator

0.1M NaOH

phenol red

TRIAMCINOLONE

ACETONIDE INJ.

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

Methanol +water (56:44)

LC1 (10 µm)

SS (25 cm x 4.6 mm)

1.4 ml per minute

254 nm

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TRIAMCINOLONE

ACETONIDE

UV visible

spectroscopy

λmax

A(1%, 1 cm)

239 nm

354

TRIAMCINOLONE

TAB.

HPLC

Mb.phase

St.phase

Flow rate

Detection λ

Methanol + Acetonitrile +

glacial acetic acid (70:30:0.1)

LC1 (10 µm)

SS (20 cm x 4 mm)

2 ml per minute

238 nm

TRIAMCINOLONE UV visible

spectroscopy

λmax

A(1%, 1 cm)

238 nm

380

TRIAMTERENE

CAPSULES

UV visible

spectroscopy

λmax

360 nm

TRIAMTERENE Non aqueous titration

A

Solvent

End-point

anh. Formic acid + anhyd.

Glacial acetic acid

Potentiometrically

TRIBASIC CALCIUM

PHOSPHATE

Complexometric

titration

Titrant

Indicator

0.05M zinc chloride


TRIFLUOPERAZINE

HCL INJ.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

256 nm

743

TRIFLUOPERAZINE

HCL TAB.

TRIFLUOPERAZINE

HCL

TRIFLUPROMAZINE

HCL


B

Solvent

Indicator


+mercuric acetate

crystal violet

TRIFLUPROMAZINE

HCL INJ.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

256 nm

700

TRIFLUPROMAZINE

HCL TAB.

TRIMETHOPRIM AND

SULPHAMETHOXAZO

LE ORAL

SUSPENSION

For trimethoprim

UV visible

spectroscopy

λmax

A(1%, 1 cm)

271 nm

204

Sulphamethox- azole-

UV visible

spectroscopy

λmax

538 nm

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TRIMETHOPRIM AND

SULPHAMETHOXAZO

LE TAB.

For trimethoprim - UV visible spectroscopy - as above

Sulphamethoxazole – nitrite titration – as in sulphamethoxazole

TRIMETHOPRIM TAB. For trimethoprim - UV visible spectroscopy - as above

TRIMETHOPRIM Non aqueous titration

A

Solvent

End-point


Potentiometrically

TRIPROLIDINE HCL

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

290 nm

290

TRIPROLIDINE HCL Non aqueous titration

B

Solvent

Indicator


1-naphtholbenzein

TROPICAMIDE EYE

DROPS

UV visible

spectroscopy

λmax

A(1%, 1 cm)

254 nm

172

TROPICAMIDE As triprolidine HCl

TROXIDONE

CAPSULES

GC Packed column

glass(0.75 m x 3 mm) with

porous polymer beads (120 to

150 µm) TROXIDONE

TUBERCULIN

PURIFIED PROTEIN

DERIVATIVE

Biological assay of tuberculin purified protein derivative

TUBOCURARINE

CHLORIDE INJ.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

280 nm

105

TUBOCURARINE

CHLORIDE

UV visible

spectroscopy

λmax

280 nm

TYPHUS VACCINE Biological assay of typhus vaccine

UNDECENOIC ACID Acid base titration Titrant

Indicator

0.5M NaOH

phenolphthalein

UREA CREAM UV visible

spectroscopy

λmax 665 nm

UREA Acid base titration Titrant

Indicator

0.1M HCl


UROKINASE Biological assay of urokinase

VANILLIN Acid base titration Titrant

End-point

1M NaOH

Potentiometrically

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VASOPRESSIN INJ. Biological assay Oxytocin.

VERAPAMIL HCL INJ. UV visible

spectroscopy

λmax

A(1%, 1 cm)

278 nm

118 VERAPAMIL HCL

TAB.

VERAPAMIL HCL Non aqueous titration

A

Solvent

End-point


+mercuric acetate

Potentiometrically

VINBLASTINE

SULPHATE INJ.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

267 nm

185

VINBLASTINE

SULPHATE

HPLC Mb.phase

St.phase

Flow rate

Detection λ

Methanol + diethylamine (1.5%

v/v pH set 7.5 with phosphoric

acid) +acetonitrile (50:38:12)

LC2 (10 µm)

SS (25 cm x 4.6 mm)

1 ml per minute

297 nm

VINCRISTINE

SULPHATE INJ.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

297 nm

177

VINCRISTINE

SULPHATE

HPLC Mb.phase

St.phase

Flow rate

Detection λ

Methanol + diethylamine (1.5%

v/v pH set 7.5 with phosphoric

acid) (70:30)

LC2 (10 µm)

SS (25 cm x 4.6 mm)

1 ml per minute

262 nm

VIPER VENOM Biological assay of snake venom

VITAMIN A

CONCENTRATE

(OILY FORM)


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VITAMIN A

CONCENTRATE

(POWDER FORM)

Assay of vitamin A, Method B

VITAMIN A

CONCENTRATE

(POWDER FORM)

VITAMINS A AND D

CAPSULES


Assay of vitamin D

WARFARIN SODIUM

TAB.

UV visible

spectroscopy

λmax

A(1%, 1 cm)

307 nm

431

WARFARIN SODIUM

WATER FOR INJ. IN

BULK

UV visible

spectroscopy

λmax

A(1%, 1 cm)

308 nm

431

WHOLE HUMAN

BLOOD

Determine Hb content by photometric haemoglobinometry

XYLOMETAZOLINE

HCL NASAL DROPS

UV visible

spectroscopy

λmax

560 nm

XYLOMETAZOLINE

HCL


B

Solvent

Indicator


+mercuric acetate

1-naphtholbenzein

XYLOSE UV visible

spectroscopy

λmax

520 nm

ZINC CHLORIDE Complexometric

titration

Titrant

Indicator

0.1M disod. Edetate

eriochrome black T

ZINC OXIDE CREAM Complexometric

titration

Titrant

Indicator

End point

0.1M disod. Edetate

xylenol orange+ hexamine

violet-pink to yellow ZINC OXIDE

ZINC STEARATE

ZINC SULPHATE EYE

DROPS

Complexometric

titration

Titrant

Indicator

0.1M disod. Edetate

mordant blackII mixture

ZINC SULPHATE As zinc oxide

ZINC UNDECENOATE

OINTMENT

For zinc undecenoate - As zinc oxide

For free undecenoic acid – as above in undecenoic acid

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REFERENCE:

* INDIAN PHARMACOPEIA 1996

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Documents

COMPILATION OF IP ASSAYSgnu.inflibnet.ac.in/bitstream/123456789/2458/1/22-Ajay Patel.pdfCOMPILATION OF IP ASSAYS A ... BENZOCAINE Nitrite titration ... Determination of esters Titrant