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Comparison of some replication components. A Problem and a Solution. Telomere Formation. A model for t loop formation. Regulation of Gene Expression in Eukaryotes. Nucleus. Protein. mRNA. (A) n. 7m G. (A) n. 7m G. (A) n. 7m G. (A) n. 7m G. Transcription. Translation. - PowerPoint PPT Presentation
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Enzyme Function
Prokaryotes Eukaryotes
Helicase
Primase
Polymerase
Proofreading
Sliding clamp
Prevent ss closure
DnaB
DnaG
Pol III
Pol III
sliding clamp
SSBPs
MCM
Primase s.u. of -polymerase
Pol and
Pol and
PCNA (Proliferating Cell Nuclear Antigen)
RPA (Replication Protein A)
Comparison of some replication components
A Problem and a Solution
Telomere Formation
A model for t loop formation
Regulation of Gene Expression in Regulation of Gene Expression in EukaryotesEukaryotes
(A)(A)nn
(A)(A)nn
(A)(A)nn
(A)(A)nn
Nucleus
mRNA
Protein
TranscriptiTranscriptionon
TranslatiTranslationonGenome Genome
OrganizatiOrganizationon
StabilityStability Post-Post-TranslatiTranslati
onon
7m7mGG7m7mGG7m7mGG7m7mGG
Reproductive cloning
Biotechnology/Cloning
Reproductive cloning
Therapeutic cloning
http://www.rndsystems.com/dam_public/6515.jpg
Therapeutic cloning
First Cloning Experiment
Cohen et al., 1973
“Recombinant DNA”
Cloning Vectors: pBR322 plasmid
Cloning into pBR322
RT-PCR and Cloning
Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615
“Supermice”
Golden Rice
Weeding Out Land MinesFirst Glowing Pet