COMPARISON OF BIOAVAILABILITY OF

DIFFERENT FORMS OF CURCUMIN USING

NON-EVERTED RAT INTESTINAL SAC MODEL

•Turmeric is dried yellow powder obtained from the rhizomes of Curcuma longa Family Zingiberaceae•Curcumin is the most plentiful of the curcuminoids found in turmeric•Biocurcumax™ is a patented extract of the rhizome of Curcuma longa, standardised to provide 95 % of natural mixed curcuminoidsand sesquiterpenoids•Curcumin is consumed as turmeric in many forms either as a spice in our daily meals or as a traditional remedy

Turmeric Rhizomes

Curcuma longa Plant

1, 7-bis (4-hydroxy-3-methoxyphenyl) -1, 6-heptadiene-3, 5- dione

• Molecular formula C21H20O6

• Molar mass 368.38 g/mol

• Curcumin is, yellow pigment, active component of turmeric

• It is orange-yellow crystalline powder, insoluble in water

• Also called diferuloyl methane

• Physicochemical properties – Practically insoluble in water– Log P : 2.05– Undergoes hydrolytic degradation at alkaline pH

• Unstable in presence of light• Curcumin is unstable in the gut and the traces that pass through the GI tract rapidly degrades or is conjugated through glucuronidation• Ingestion of 2 to 10 grams of curcumin resulted in undetectable to very low serum levels• Limited clinical application due to poor aqueous solubility, poor absorption and extensive metabolism leading to decreased bioavailability

• As spices and colorant• As cosmetic • As Blood purifier• As a remedy for ulcer Common cold, wound, Sore throat, leprosy inflammation etc.

• Anticancer activity• Antioxidant activity• Antidiabetic activity• Antirheumatic activity• Antimicrobial activity• Hepatoprotective and Nephroprotective activity

COMPARISON OF BIOAVAILABILITY OF DIFFERENT FORMS OF CURCUMIN USING NON-EVERTED

RAT INTESTINAL SAC MODEL

BIOCURCUMAX

PURE CURCUMIN

TURMERIC

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• Male wistar rats (weighing 250-300 g) were used for the study• The clean intestinal tract was prepared into 8 ± 0.2 cm long sacs

having a diameter of 3 ± 0.5 mm• Each sac was filled with 0.5 ml of drug suspension and tied with

thread to a length of approximately 5 cm• Each non-everted intestinal sac was placed in a glass conical flask

containing KRPBS (50 ml) with provision for aeration• The sacs were maintained at 37 °C in a shaking water bath

operating at 50 strokes per min• Samples were withdrawn from outside the sacs every 30 min for 8 h and replaced with fresh buffer• The samples were analyzed spectrophotometrically at λmax 422nm

Graphs showing % Permeation in 8 h from different vehicles

Results of the study indicate that: - % Permeation in 8 h from the rat intestine was maximum for Corn oil from all the forms of curcumin - The pattern of % Permeation profile for different forms of

curcumin from Corn oil is: Biocurcumax(26.3) > Turmeric(19.9)> Pure curcumin(13.4) - The pattern for % Permeation profile from different vehicles is: Corn oil(26.3)> R.Butter(16.9)> Milk(6.8)> Aq. Suspension(2.5)

COMPARITIVE PERMEATION PROFILE OF DIFFERENT FORMS OF CURCUMIN FROM DIFFERENT VEHICLES

The Present study concludes that: - % Permeation of curcumin increase as the lipophilicity of the vehicle increases - Permeation is maximum from Biocurcumax followed by Turmeric and Pure curcumin - Curcumin should be consumed with oily vehicles instead of water as Biocurcumax or Turmeric for its maximum therapeutic effects

REFERENCES:1. Hatcher H, Planalp R, Cho J, Torti FM, Torti SV, Curcumin: from ancient medicine to current clinical trials, Cell. Mol. Life Sci., 2008, 65 (11): 1631–522. Ruan LP, Chen S, Yu BY, Zhu DN, Cordell GA, Qiu SX, Prediction of human absorption of natural compounds by the non-everted rat intestinal sac model, Eur. J. Med. Chem., 2006, 41: 605-610

REFERENCES:1. Hatcher H, Planalp R, Cho J, Torti FM, Torti SV, Curcumin: from ancient medicine to current clinical trials, Cell. Mol. Life Sci., 2008, 65 (11): 1631–522. Ruan LP, Chen S, Yu BY, Zhu DN, Cordell GA, Qiu SX, Prediction of human absorption of natural compounds by the non-everted rat intestinal sac model, Eur. J. Med. Chem., 2006, 41: 605-610

curcumin

turmeric