College of Agriculture and Life Sciences

Department of Plant Breeding

Anthracnose Stalk Rot Resistancefrom GEM Germplasm

Margaret Smith

Department of Plant Breeding

Cornell University

College of Agriculture and Life Sciences

Department of Plant Breeding

Anthracnose Stalk Rot

• Colletotrichum graminicola (Ces.) G.W. Wils.

• Increased lodging, reduced yield

• Best control through resistant varieties

• Limited resistance in temperate germplasm

College of Agriculture and Life Sciences

Department of Plant Breeding

Overall Project Objective

Develop temperate-adapted maize inbreds

with both anthracnose stalk rot resistance and

good yield potential

from GEM breeding populations

College of Agriculture and Life Sciences

Department of Plant Breeding

Selection and Breeding SchemeEvaluate 30 populations

for ASR resistance

Testcross and evaluateyield potential and adaptation

Self pollinate and screen for ASR resistance

Testcross to monitoryield and adaptation

College of Agriculture and Life Sciences

Department of Plant Breeding

Selected Populations

1) FS8B(T):N1802

2) CH04030:S0906

3) AR01150:N0406

4) GOQUEEN:N1603

College of Agriculture and Life Sciences

Department of Plant Breeding

Means by source population for anthracnose stalk rot ratings, 2001

Means of S5 Means of selectedfamily ratings (%) S5 plant ratings (%)

No. Mean No. MeanPopulation Families (± s.d.) Plants (± s.d.) FS8B(T):N1802 6 11.7 (± 1.8) 6 14.0 (± 5.3)CH04030:S0906 9 11.5 (± 1.6) 12 12.6 (± 7.5)AR01150:N0406 6 16.1 (± 4.2) 9 14.5 (± 7.4)GOQUEEN:N1603 9 16.0 (± 6.6) 13 10.6 (± 9.4)

Resis. check (01/31*) 48.0 LB31B 50.6Susc. check (B37) 73.2 * Our most resistant inbred at present, currently being released.

College of Agriculture and Life Sciences

Department of Plant Breeding

Yield Data - 2 locations, 2001Yield Moist. Y/M Lodging (%)

S2 Family Tester (bu/A) (%) Ratio Root Stalk

FS8B(T):N1802-490 B73/CD1 118.5 21.9 5.6 0 19FS8B(T):N1802-491 “ 122.8 21.7 5.8 1 16FS8B(T):N1802-492 “ 119.7 26.0 4.7 0 13FS8B(T):N1802-493 “ 123.5 25.0 5.0 0 16CH04030:S0906-476 RD6501/ 118.5 22.7 5.2 0 45CH04030:S0906-477 RD6502 120.1 21.7 5.6 0 31CH04030:S0906-478 “ 112.5 21.8 5.2 0 24AR01150:N0406-496 B73/CD1 112.9 20.8 5.5 0 43

Pioneer 34G81 166.1 20.0 8.4 8 7Agway 795 128.1 25.0 5.2 0 7Doeblers 596XY 125.3 21.1 6.0 0 15

Coefficient of variation (%) 13.3 5.5

College of Agriculture and Life Sciences

Department of Plant Breeding

Yield Data - 1 location, 2001Yield Moist. Y/M Lodging (%)

S2 Family Tester (bu/A) (%) Ratio Root Stalk

AR01150:N0406-498 B73/CD1 164.1 22.0 7.5 0 3AR01150:N0406-499 “ 187.5 21.8 8.6 0 8GOQUEEN:N1603-510 “ 197.7 20.1 9.9 0 3GOQUEEN:N1603-511 “ 194.0 19.0 10.2 0 6

Pioneer 34G81 214.5 19.6 10.9 0 0Agway 657 193.3 20.5 9.4 0 4Agway 795 183.6 24.0 7.6 0 3Doeblers 596XY 169.5 20.2 8.4 0 3

Coefficient of variation (%) 10.8 4.9

College of Agriculture and Life Sciences

Department of Plant Breeding

Work done in 2002…

• S6 progenies planted ear-to-row– Greater emphasis on yield performance– Greater tolerance of stalk rot resistance

• Testcrosses made to:– Previously used public line crosses– Holden’s testers (LH176/LH177, LH198)

College of Agriculture and Life Sciences

Department of Plant Breeding

Anthracnose Stalk Rot Resistance in a Few GEM

Inbreds, 2002 Internodes 2 through 8 (%) Mean of injected Plant Plant

Inbred internode (%) Mean (± s.d.) Min. Max.FS8B(T):N1802 15-255 78.3 9.4 (± 9.4) 0.2 25.3FS8B(T):N1802 15-259 90.7 23.2 (± 13.8) 7.6 46.0CH04030:S0906 15-242 90.3 15.7 (± 10.4) 1.8 28.9GOQUEEN:N1603 15-276 68.5 7.1 (± 5.5) 0.2 17.0RD02LB1-2 (resis. check) 56.2 5.2 (± 5.6) 0.2 15.3DE811 ASR (resis. check) 42.6 9.1 (± 7.8) 1.3 21.9RD6501 (resis. check) 61.3 16.3 (± 2.9) 14.5 19.6NYLB31B (resis. check) 86.8 26.5 (± 17.5) 2.0 49.4B37 (suscep. check) 91.4 70.6 (± 9.1) 58.9 83.1

College of Agriculture and Life Sciences

Department of Plant Breeding

FS8B(T):N1802 15-255

DE811ASR B37

College of Agriculture and Life Sciences

Department of Plant Breeding

FS8B(T):N1802 15-255

DE811ASR B37

College of Agriculture and Life Sciences

Department of Plant Breeding

Yields of a Few GEM Inbreds Tested on B73/CD1, 2001, 2002

Yield Moisture Y/M Root StalkS5 Family (bu/A) (%) Ratio Lodge (%) Lodge (%)FS8B(T):N1802-490 129.1 25.4 5.1 3 15FS8B(T):N1802-491 129.2 26.2 4.9 11 13GOQUEEN:N1603-512 146.3 23.9 6.1 3 16GOQUEEN:N1603-510 133.9 23.9 5.6 8 14GOQUEEN:N1603-511 133.9 23.1 5.8 7 20

Pioneer 34G81 170.5 23.4 7.3 2 7Doeblers 596XY 156.5 25.2 6.2 2 13

Coefficient of variation (%) 13.8 2.4

College of Agriculture and Life Sciences

Department of Plant Breeding

Work done in 2003…

• S6 progenies and their testcrosses (public and Holden’s testers) evaluated for anthracnose stalk rot resistance (2 reps)

• Testcrosses evaluated in yield trials in 3 New York locations (2 reps/location)

• Results: just in from the field -- stay tuned!

College of Agriculture and Life Sciences

Department of Plant Breeding

Thank you! Questions?