Collagen and Gelatin Product Guide Feb. 2020 › en › biomedical › ... · Collagen for Cell Culture Collagen solution at pH 3 with a concentration of 3 mg/mL Storage condition:

Living with the Cells.

Evolving Biomaterial – Collagen and Gelatin Future Healthcarewith

Collagen and Gelatin

Life Science Products

Product GuideCollagen and Gelatin

Collagen for Cell Culture

■ Collagen solution at pH 3 with a concentration of 3 mg/mL■ Storage condition: Cold storage (4°C to 8°C)

Collagen for Three-dimensional Culture / Collagen for Coating

Cellmatrix® Type I -A･ Ideal for collagen gel culture.･ The gel has high transparency and ensures ease of microscopic observation.･ Acid extracted collagen derived from porcine tendons.･ Expiration date: One year from the date of manufacture

･ Recommended for collagen gel culture and collagen- coated culture.･ Pepsin-solubilized collagen derived from porcine tendons.･ Expiration date: One year from the date of manufacture

･ Recommended for collagen-coated culture.･ Pepsin-solubilized collagen derived from porcine skin.･ Expiration date: Two years from the date of manufacture

･ Recommended for collagen-coated culture.･ Non gelling form.･ Type III collagen derived from porcine skin.･ Expiration date: Two years from the date of manufacture

･ Recommended for collagen-coated culture.･ Non gelling form.･ Type IV collagen derived from bovine lens capsules purified with pepsin treatment.･ Expiration date: Two years from the date of manufacture

Adhesion of cultured cells

Uncoated Collagen coated

Comparison of Transparency

Type I-A is a collagen product with an optimized gelation rate and transparency for 3-dimensional culture. It can quickly form a gel before the cells sink to the bottom of the culture dish. In addition, the gel is highly transparent, allowing easy observation of the cells embedded in the collagen gel.

Type I-A Competitors products

Growth morphology of mouse breast cancer cells cultured in an embedded collagen gel

Cellmatrix® Type I -P

Cellmatrix® Type I -C

Cellmatrix® Type III

Cellmatrix® Type IV

Concentrated Culture Solution

Collagen Gel Culturing Kit

Buffer Solution for Reconstitution

Cellmatrix products list

Collagen Gel Culturing Kit / Concentrated Culture Solution /Buffer Solution for Reconstitution

■ Storage condition: Cold storage (4°C to 8°C) ■ Expiration date: In four months

■ ContentsCellmatrix Type I-A 20 mL One bottleConcentrated culture solution (Ham’s F-12 and MEM) 5 mL each One bottle eachBuffer solution for reconstitution 4 mL Five bottles

Concentrated culture solution adjusted for collagen gel culture,Ham's F-12 culture solution as a 10x concentrated product,MEM-Hanks' culture solution, DF culture solution (DME:F-12=1:1),199 culture solution, DME culture solution as 5x concentratedproduct, and RPMI-1640 culture solution are available.

Buffer solution for reconstitution, adjusted for collagengel culture■ Composition

Sodium hydroxide 50mMSodium hydrogen carbonate 260mMHEPES 200mM

Product name Content

Cellmatrix Type I-A 20 mL 100 mL

Cellmatrix Type I-P 20 mL 100 mL

Cellmatrix Type I-C 20 mL 100 mL

Cellmatrix Type III 5 mL

20 mL 100 mL

Cellmatrix Type IV 5 mL

20 mL 100 mLCollagen gel culturing kit 1 kitHam’s F-12 concentrated culture solution 100 mLMEM-Hank’s concentrated culture solution 100 mLDF concentrated culture solution 100 mL199 concentrated culture solution 100 mLDME concentrated culture solution 100 mLRPMl-1640 concentrated culture solution 100 mLBuffer solution for reconstitution 4mL 15bottles

Concentrated Culture Solution

Collagen Gel Culturing Kit

Buffer Solution for Reconstitution

For Research Use OnlyThe above products are sold for research purposes only. Regardless of purposes, such as the production of drugs, product quality control, various diagnostic tests, medical treatment, or other researches, do not use the products for the human body.Warning

Method of Collagen Gel Culture

Method of Collagen Coating

Culture on collagen gel

A method of three-dimensional cellculture in collagen gel

* Adjust thedilution ratiodepending onthe purpose.

Prepare the following three types of solutions, i.e, A, B, and C.

A: Cellmatrix Type I-A or I-P B: 10x concentrated Culture Solution1)

C: Buffer Solution for ReconstitutionMix A and B well at a ratio of 8 to 1 while cooling A and B so that the mixture does not foam. Then add C at a ratio of 1 to previous mixture and mix them together.1)

1) In the case of using culture solution at a concentration of approximately 5x, the following mixture ratio will apply: A:B:C=7:2:1

The above collagen mixture will turn into a gel when the solution is dispensed to a culture dish and heated at 37°C for 30 minutes. Keep the mixture cold before incubation or during mixing.

After gelation spread the cell dispersion onto the gel.Once the cells adhere to the gel, it will be possible toculture the cells like normal monolayer culture.

This is a method to culture the cellson the collagen gel.

Add the resultant mixture (A+B+C) mentioned bove to cell pellet obtained after centrifugation and mix well.

Dispense this collagen mixture solution to a culture dish and leave the solution at 37°C for 30 minutes sothat the solution will turn into a gel.

After the gel is formed, overlay with culture solutionand perform culture in an ordinary manner.

Collagen gel-embedded culture

pH3 hydrochloricacid solutionthat is 10 timesthe amount ofcollagen*

CellmatrixType I-CI-P, III, or IV

Dry the collagen solution in a cleanbench. Leave thecollagen solution for30 to 60 minutes.

Wash the coated dish twice with theculture solution.

Perform culturelike monolayerculture.

Dispense and thinlyspread the dilutedcollagen solution toa dish and aspiratethe excessiveamount of collagen.

Collagen for Cell Culture

Method of Collagen Gel Culture

Method of Collagen Coating

Gelatin / Collagen for Tissue Engineering

Low Endotoxin Gelatin■ Endotoxin level: NMT 10EU/g ■ Virus inactivated■ IPEC-PQG GMP Compliant ■ USP/EP/JP compliant ■ DMF/MAF registered　■ Storage condition: Room temperature■ Expiration date: Three years from the date of manufacture


Sensitization

Negative

IntradermalReaction

Negative

Pyrogen

Negative

Cytotoxicity

Negative

Antigenicity

Negative

TestItem

Result

Sensitization

Negative

IntradermalReaction

Negative

Pyrogen

Negative

Cytotoxicity

Negative

Antigenicity

Negative

TestItem

Result

AcuteSystemicToxicityNegative

SubacuteToxicity

Negative


beMatrix Gelatin LS-H 10gbeMatrix Gelatin LS-250 10gbeMatrix Gelatin LS-W 10gbeMatrix Gelatin HG 10g

beMatrix products list

beMatrix® Gelatin HG

・Sterilized gelatin hydrolyzate derived from porcine skin・Safety Test

beMatrix® Gelatin LS - 250

・Sterilized alkaline-treated gelatin derived from porcine skin・High Gel strength

beMatrix® Gelatin LS -H

・Alkaline-treated gelatin derived from porcine skin・High Gel strength

beMatrix® Gelatin LS -W

・Alkaline-treated gelatin derived from porcine skin・Low Gel strength・Safety Test


beMatrix Collagen AT 100mLbeMatrix Collagen TE 100mLbeMatrix Collagen FD 1g

beMatrix Collagen products list

Low Endotoxin Collagen

beMatrix® Collagen ATAcid extracted collagen solution derived from porcine tendon■ Low endotoxin level (NMT 0.5EU/mL)■ Storage condition: Cold storage (4°C to 8°C)■ Expiration date: Two years from the date of manufacture ■ Concentration: 3mg/mL, pH3

beMatrix® Collagen TEPepsin-solubilized collagen solution derived from porcine skin■ Low endotoxin level (NMT 0.5EU/mL)■ Storage condition: Cold storage (4°C to 8°C)■ Expiration date: Two years from the date of manufacture ■ Concentration: 5mg/mL, pH3

Gelatin / Collagen for Tissue Engineering

beMatrix® Collagen FDFreeze-dried atelocollagen derived from porcine skin■ Low endotoxin level (NMT 100EU/g)■ Storage condition: Room temperature■ Expiration date: Two years from the date of manufacture

・Virus inactivated product・High solubility in water

Product appearance・Spongiform・Size: approx.140x100x10mm

Application・Scaffold・Cell culture / transplantation・Electrospinning etc.


MedGel® is a hydrogel for sustained release of drugs

Gelatin-based Hydrogel for Drug Delivery System

・ Storage condition: Room temperature・ Best before: Two years from the date of manufacture・ pI (isoelectric point): approximately 5 negative charge in neutral solution

■ Simple use just by adding drug.■ Drug stabilization in vivo, site-specific drug release.■ No chemical crosslinking agent used.

MedGel® Sheet II (PI5)

MedGel® Sheet II (PI9)


MedGel Sheet II (PI5) 150mgMedGel Sheet II (PI9) 150mg

MedGel Particle II (PI5) 15mg 2bottles

100mg 1bottle

Gelatin Cross-linking

Water

Drug

Gelatin Particles(negative charge)

Drug(positive charge)

added

When a drug (positive charge) is added to gelatin particles (negative charge) they are bonded to each other by the electrostatic interaction.

in vivo

After being administered in vivo, the drug is locally released for a certain period as the gelatin is dissolved.

Mechanism of Drug delivery through MedGel II

MedGel® is a registered trademark of MedGEL Corporation. These products are licensed to manufacture and sell from MedGEL Corporation.

* MedGel Particle II (PI5) Case Study


MedGel II products list

・ Storage condition: Room temperature・ Best before: Two years from the date of manufacture・ pI (isoelectric point): approximately 9 positive charge in neutral solution

・ Storage condition: Room temperature・ Best before: Two years from the date of manufacture・ pI (isoelectric point): approximately 5 negative charge in neutral solution

MedGel® Particle II (PI5)

Other Products Line-up

Collagen Sponge

Collagen BM

Other Products Line-up

Collagen BM

■ Storage condition: Room temperature■ Expiration date: Two years from the date of manufacture・Pepsin-solubilized collagen derived from porcine skin.・γ-ray sterilization.・Made to order product.

24-well-plate use35-mm-dish use 12-well-plate use

■ Storage condition：Stored frozen■ Expiration date: Two years from the date of manufacture・Pepsin-solubilized collagen derived from porcine skin.・Concentration: 5mg/mL, pH3

Collagen Sponge


Collagen Sponge 35-mm-dish use (approx. 32 dia.x 5 mm) 6 piece/case

Collagen Sponge 12-well-plate use (approx. 20 dia.x 3 mm) 12 piece/case

Collagen Sponge 24-well-plate use (approx. 15 dia.x 3 mm) 24 piece/case

Collagen BM 1 kg

Products list


Collected Papers for “Cellmatrix” (10 papers in each year)2019 Journal Vol (No) Page Year Author(s) Title 1 Bone 103 56 2019 Sakamoto M., et al. Vibration enhances osteoclastogenesis by inducing RANKL expression via NF-κB signaling in osteocytes. 2 Nature Communications 10 (5446) 558 2019 Kobayashi S., et al. Enhanced lysosomal degradation maintains the quiescent state of neural stem cells 3 Scienti�c Reports 9 (20056) 2019 Hirakawa T. β-catenin signaling inhibitors ICG-001 and C-82 improve �brosis in preclinical models of endometriosis 4 Biomaterials 197 305 2019 Usuba R., et al. EGFL7 regulates sprouting angiogenesis and endothelial integrity in a human blood vessel model 5 Cancers 11 (5) 604 2019 Sato Y., et al. High Mannose Binding Lectin (PFL) from Pseudomonas �uorescens Down-Regulates Cancer-Associated Integrins and Immune Checkpoint Ligand B7-H4 6 Biomaterials Science 7 3460 2019 Tan A., et al Probing cell–nanoparticle (cubosome) interactions at the endothelial interface: do tissue dimension and �ow matter? 7 Molecular Oncology 13 1706 2019 Akatsu Y., et al Fibroblast growth factor signals regulate transforming growth factor-b-induced endothelial-to-myo�broblast transition of tumor endothelial cells via Elk1 8 Carbohydrate Polymers 214 303 2019 Li W., et al Anti-obesity effects of chondroitin sulfate oligosaccharides from the skate Raja pulchra 9 Nat Protocols 14 1323 2019 Toyoshima K., et al. Regeneration of a bioengineered 3D integumentary organ system from iPS cells 10 Materialstoday Chemistry 13 8 2019 Ishikawa Y., et al Cellular morphologies, motility, and epithelial–mesenchymal transition of breast cancer cells incubated on viscoelastic gel substrates in hypoxia

2018 Journal Vol (No) Page Year Author(s) Title 1 Nature 561 (7722) 243 2018 Kurita M., et al In vivo reprogramming of wound-resident cells generates skin epithelial tissue. 2 Nature Communications 9 4695 2018 Anton KA., et al Src-transformed cells hijack mitosis to extrude from the epithelium. 3 Biomaterials 161 24 2018 Takayama K., et al Enrichment of high-functioning human iPS cell-derived hepatocyte-like cells for pharmaceutical research. 4 Science advances 4 (10) eaat5847 2018 Osaki T., et al Microphysiological 3D model of amyotrophic lateral sclerosis (ALS) from human iPS-derived muscle cells and optogenetic motor neurons. 5 Scienti�c Reports 8 10071 2018 Kimura H., et al Stem cells puri�ed from human induced pluripotent stem cell-derived neural crest-like cells promote peripheral nerve regeneration. 6 Scienti�c Reports 8 9229 2018 Fukuda T., et al Expression of human mutant cyclin dependent kinase 4, Cyclin D and telomerase extends the life span but does not immortalize �broblasts derived from loggerhead sea turtle (Caretta caretta). 7 Colloid and Surfaces A 546 136 2018 Peanparkdee M., et al Stability of bioactive compounds from Thai Riceberry bran extract encapsulated within gelatin matrix during in vitro gastrointestinal digestion. 8 EBioMedicine 27 225 2018 Pauty J., et al A Vascular Endothelial Growth Factor-Dependent Sprouting Angiogenesis Assay Based on an In Vitro Human Blood Vessel Model for the Study of Anti-Angiogenic Drugs. 9 Gastric Cancer 21 (6) 946 2018 Akutagawa T., et al Cancer-adipose tissue interaction and �uid �ow synergistically modulate cell kinetics, HER2 expression, and trastuzumab ef�cacy in gastric cancer. 10 Sensors & Actuators: B 273 1062 2018 Matsumoto S., et al Integration of an oxygen sensor into a polydymethylsiloxane hepatic culture device for two-dimensional gradient characterization.

2017 Journal Vol (No) Page Year Author(s) Title 1 Nature Cell Biology 19 530 2017 Kon S., et al. Cell competition with normal epithelial cells promotes apical extrusion of transformed cells through metabolic changes. 2 Nature Communications 44 (2) 140 2017 Ohno Y., et al. PNPLA1 is a transacylase essential for the generation of the skin barrier lipid ω-O-acylceramidecollagen. 3 PNAS 114 (12) e2476 2017 Enoki R., et al. Synchronous circadian voltage rhythms with asynchronous calcium rhythms in the suprachiasmatic nucleus. 4 Acta Biomaterialia 48 489 2017 Wang Z., et al. TiAl 6 V 4 particles promote osteoclast formation via autophagy-mediated downregulation of interferon-beta in osteocytes. 5 Oncotarget 8 (38) 63258 2017 Huan B., et al. Effect of wound �uid on chemotherapy sensitivity of T24 bladder cancer cells with different enhancer of zeste homolog 2 status. 6 Scienti�c Reports 7 42426 2017 Takahashi H., et al. Visualizing dynamics of angiogenic sprouting from a three-dimensional microvasculature model using stage-top optical coherence tomography. 7 Tissue Engineering Part C 23 (6) 357 2017 Tsukamoto Y., et al. Fabrication of Orientation-Controlled 3D Tissues Using a Layer-by-Layer Technique and 3D Printed a Thermoresponsive Gel Frame. 8 Tissue Engineering Part C 23 (7) 396 2017 Han H., et al. Development of In Vitro Embryo Production System Using Collagen Matrix Gel Attached with Vascular Endothelial Growth. Factor Derived from Interleukin-1 Beta-Treated Porcine Endometrial Tissue. 9 Cellular Physiology 233 (1) 201 2017 Dunon M. J., et al. Transforming growth factor β induces bone marrow mesenchymal stem cell migration via noncanonical signals and N-cadherin. 10 Scienti�c Reports 7 41733 2017 Enoki R., et al. Dual origins of the intracellular circadian calcium rhythm in the suprachiasmatic nucleus.

2016 Journal Vol (No) Page Year Author(s) Title 1 Nat Biotechnol 34 (7) 752 2016 Quarta M., et al. An arti�cial niche preserves the quiescence of muscle stem cells and enhances their therapeutic ef�cacy. 2 Science Advances 2 (4) e1500887 2016 Takagi R., et al. Bioengineering a 3D integumentary organ system from iPScells using an in vivo transplantation model. 3 Nature Communications 7 11349 2016 Okamoto M., et al. Cell-cycle-independent transitions in temporal identity of mammalian neural progenitor cells. 4 Scienti�c Reports 6 22071 2016 Tanimoto R., et al. Detection of Temperature Difference in Neuronal Cells. 5 Scienti�c Reports 6 28383 2016 Chiba T., et al. MDCK cells expressing constitutively active Yes-associated protein (YAP) undergo apical extrusion depending on neighboring cell status. 6 Proc Natl Acad Sci USA 113 (1) E71 2016 Nishio M., et al. Dysregulated YAP1/TAZ and TGF-β signaling mediatehepatocarcinogenesis in Mob1a/1b-de�cient mice. 7 Oncogenesis 4 e181 2016 Kamino H., et al. Mieap-regulated mitochondrial quality control is frequentlyinactivated in human colorectal cancer. 8 Nucleic Acids Res 44 (11) 5105 2016 Yamamoto N.,et al. Bidirectional promoters link cAMP signaling with irreversibledifferentiation through promoter-associated non-coding RNA (pancRNA) expression in PC12 cells. 9 PLoS One 11 (8) e0162049 2016 Matsumoto T., et al. Retinol Promotes In Vitro Growth of Proximal Colon Organoids through a Retinoic Acid-Independent Mechanism. 10 Tissue Engineering Part C 22 (1) 20 2016 Yamazoe H., et al. Multicomponent Coculture System of Cancer Cells and Two Types of Stromal Cells for In Vitro Evaluation of Anticancer Drugs.

2015 Journal Vol (No) Page Year Author(s) Title 1 Nat Biotechnol 33 (8) 853 2015 Ogawa M., et al. Directed differentiation of cholangiocytes from human pluripotent stem cells. 2 Nature Communications 6 6373 2015 Ito K., et al. Arti�cial human Met agonists based on macrocycle scaffolds. 3 Nature Communications 6 8021 2015 Ishii T., et al. Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. 4 Scienti�c Reports 5 7656 2015 Yamaguchi N., et al. Leader cells regulate collective cell migration via Rac activation in the downstream signaling of integrin β1 and PI3K. 5 Biomaterials 72 29 2015 Aya R., et al. Regeneration of elastic �bers by three-dimensional culture on a collagen scaffold and the addition of latent TGF-β binding. protein 4 to improve elastic matrix deposition. 6 Biomaterials 51 278 2015 Yoshii Y., et al. High-throughput screening with nanoimprinting 3D culture for ef�cient drug developmenty mimicking the tumor environment. 7 J Biol Chem 290 (10) 6086 2015 Aki S., et al. Phosphatidylinositol 3-kinase class II α-isoform PI3K-C2α is required for transformin”g growth factor α-induced Smad signaling in endothelial cells. 8 FEBS Open Bio 4 229 2015 Mitsuda S., et al. Ursolic acid, a natural pentacyclic triterpenoid, inhibits intracellular traf�cking of proteins and inducesaccumulation of intercellular adhesion molecule-1 linked to high-mannose-type glycans in the endoplasmic reticulum. 9 Oncogene 34 (24) 3176 2015 Satoyoshi R., et al. Tks5 activation in mesothelial cells creates invasion front of peritoneal carcinomatosis. 10 Acta Biomaterialia 11 449 2015 Kang Y., et al. Engineering a vascularized collagen-β-tricalcium phosphate graft using an electrochemical approach.

Collected Papers for “Cellmatrix” (10 papers in each year)2014 Journal Vol (No) Page Year Author(s) Title 1 PLoS One 9 (1) 85132 2014 Ehashi T., et al. Comprehensive genetic analysis of early host body reactions to the bioactive and bio-inert porous scaffolds. 2 Stem Cell Reports 2 (2) 205 2014 Kamao H., et al. Characterization of human induced pluripotent stem cell-derived retinal pigment epithelium cell sheets aiming for clinical application. 3 Integr Biol (Camb) 6 (2) 127 2014 DiMarco RL., et al. Engineering of three-dimensional microenvironments to promote contractile behavior in primary intestinal organoids. 4 PLoS One 9 (1) 86186 2014 Tonosaki M., et al. L1cam Is Crucial for Cell Locomotion and Terminal Translocation of the Soma in Radial Migration during Murine Corticogenesis. 5 Biomaterials 35 (7) 2172 2014 Jiang N., et al. Postnatal epithelium and mesenchyme stem/progenitor cells in bioengineered amelogenesis and dentinogenesis 6 Proc Natl Acad Sci USA 11 (47) 16772 2014 Takayama K., et al. Prediction of interindividual differences in hepatic functions and drug sensitivity by using human iPS-derived hepatocytes. 7 Stem Cells 32 (12) 3266 2014 Asai S., et al. Tendon progenitor cells in injured tendons have strong chondrogenic potential: the CD105-negative subpopulation induces chondrogenic degeneration. 8 J Biol Chem 289 (16) 11545 2014 Hayashida C., et al. Osteocytes produce interferon-β as a negative regulator of osteoclastogenesis. 9 Scienti�c Reports 4 4867 2014 Yajima-Himuro S., et al. The junctional epithelium originates from the odontogenic epithelium of an erupted tooth. 10 Scienti�c Reports 4 4568 2014 Yuki K., et al. E-cadherin–downregulation and RECK-upregulation are coupled in the non-malignant epithelial cell line MCF10A but not in multiple carcinoma-derived cell lines.

2013 Journal Vol (No) Page Year Author(s) Title 1 Scienti�c Reports 3 1418 2013 Kito T., et al. iPS cell sheets created by a novel magnetite tissue engineering method for reparative angiogenesis. 2 Scienti�c Reports 3 1144 2013 Nagai H., et al. Metamorphosis of mesothelial cells with active horizontal motility in tissue culture. 3 Scienti�c Reports 342 (6163) 1203 2013 Imayoshi I., et al. Oscillatory control of factors determining multipotency and fate in mouse neural progenitors. 4 Proc Natl Acad Sci USA 110 (8) 2852 2013 Noda K., et al. Latent TGF-{beta} binding protein 4 promotes elastic �ber assembly by interacting with �bulin-5. 5 Cell Struct Funct 38 (2) 227 2013 Toki F., et al Second Harmonic Generation Reveals Collagen Fibril Remodeling in Fibroblast-populated Collagen Gels. 6 PLoS One 8 (8) 66751 2013 Ding T., e al. Knockdown a water channel protein, aquaporin-4, induced glioblastoma cell apoptosis. 7 PLoS One 8 (8) 71252 2013 Koriyama Y., et al. Requirement of retinoic acid receptor β for genipin derivative-induced optic nerve regeneration in adult rat retina. 8 PLoS One 8 (10) 78061 2013 Kim A., et al. Aqueous extract of Bambusae Caulis in Taeniam inhibits PMA-induced tumor cell invasion and pulmonary metastasis: suppression of NF-κB activation through ROS signaling. 9 J Biol Chem 288 19958 2013 Tashiro E., et al. Prefoldin Protects Neuronal Cells from Polyglutamine Toxicity by Preventing Aggregation Formation. 10 J Biol Chem 288 35126 2013 Woskowicz AM., et al. MT-LOOP-dependent Localization of Membrane Type I Matrix Metalloproteinase (MT1-MMP) to the Cell Adhesion Complexes Promotes Cancer Cell Invasion.

2012 Journal Vol (No) Page Year Author(s) Title 1 Proc Natl Acad Sci USA 109 (41) 16449 2012 Harris AR., et al. Characterizing the mechanics of cultured cell monolayers. 2 Proc Natl Acad Sci USA 109 (52) 21498 2012 Enoki R., et al. Topological speci�city and hierarchical network of the circadian calcium rhythm in the suprachiasmatic nucleus. 3 Proc Natl Acad Sci USA 109 (52) 21426 2012 Lipinski S., et al. RNAi screening identi�es mediators of NOD2 signaling: implications for spatial speci�city of MDP recognition. 4 J Invest Dermatol 132 (7) 1775 2012 Ishitsuka Y., et al. Pituitary tumor-transforming gene 1 enhances proliferation and suppresses early differentiation of keratinocytes. 5 Nature Neuroscience 15 (9) 1201 2012 Yang YT., et al. DOCK7 interacts with TACC3 to regulate interkinetic nuclear migration and cortical neurogenesis. 6 Journal of Cell Science 125 (24) 6127 2012 Hashimoto T., et al. Active involvement of micro-lipid droplets and lipid-droplet-associated proteins in hormone-stimulated lipolysis in adipocytes. 7 Tissue Engineering Part A 18 (15-16) 1677 2012 Wen Y., et al. Application of induced pluripotent stem cells in generation of a tissue-engineered tooth-like structure. 8 Langmuir 28 (9) 4404 2012 Ogawa Y., et al. Helical assembly of azobenzene-conjugated carbohydrate hydrogelators with speci�c af�nity for lectins. 9 FEBS Journal 279 (10) 1857 2012 Ishii S., et al. The response of PKD1L3/PKD2L1 to acid stimuli is inhibited by capsaicin and its pungent analogs. 10 Neuron 73 (5) 911 2012 Cappello S., et al. A radial glia-speci�c role of RhoA in double cortex formation.

2011 Journal Vol (No) Page Year Author(s) Title 1 Proc Natl Acad Sci USA 108 (15) 6235 2011 Kondo　J., et al. Retaining cell–cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer. 2 J Biol Chem 286 (4) 3094 2011 Sugita A., et al. Cellular ATP Synthesis Mediated by Type III Sodium-dependent Phosphate Transporter Pit-1 Is Critical to Chondrogenesis. 3 J Biol Chem 286 (20) 17536 2011 Nishitsuji K., et al. Apolipoprotein E Regulates the Integrity of Tight Junctions in an Isoform-dependent Manner in an in Vitro Blood-Brain Barrier Model. 4 J Biol Chem 286 (14) 1524 2011 Sato Y., et al. Cellular Hypoxia of Pancreatic β-Cells Due to High Levels of Oxygen Consumption for Insulin Secretion in Vitro. 5 J Biol Chem 286 (10) 8055 2011 Zhang J., et al. Angiopoietin-1/Tie2 Signal Augments Basal Notch Signal Controlling Vascular Quiescence by Inducing Delta-Like 4 Expression through AKT-mediated Activation of β-Catenin. 6 J Biol Chem 286 (44) 38602 2011 Saito M., et al. ADAMTSL6β Protein Rescues Fibrillin-1 Micro�bril Disorder in a Marfan Syndrome Mouse Model through the Promotion of Fibrillin-1 Assembly. 7 J Biol Chem 286 (9) 7587 2011 Tochowicz A., et al. The Dimer Interface of the Membrane Type 1 Matrix Metalloproteinase Hemopexin Domain: Crystal Structure and Biological Functions. 8 Tissue Engineering Part A 17 (5-6) 597 2011 Tachi K., et al. Enhancement of Bone Morphogenetic Protein-2-Induced Ectopic Bone Formation by Transforming Growth Factor-β1. 9 The EMBO Journal 30 (4) 783 2011 Shirakihara T., et al. TGF-β regulates isoform switching of FGF receptors and epithelial–mesenchymal transition. 10 PLoS One 6 (7) 21531 2011 Oshima M., et al. Functional Tooth Regeneration Using a Bioengineered Tooth Unit as a Mature Organ Replacement Regenerative Therapy.

2010 Journal Vol (No) Page Year Author(s) Title 1 Proc Natl Acad Sci USA 107 (10) 4601 2010 Umezu T., et al. Follistatin-like-1, a diffusible mesenchymal factor determines the fate of epithelium. 2 J Biol Chem 285 (47) 36745 2010 Adapala NS, et al. The Loss of Cbl-Phosphatidylinositol 3-Kinase Interaction Perturbs RANKL-mediated Signaling, Inhibiting Bone Resorption and Promoting Osteoclast Survival. 3 J Biol Chem 285 (43) 33540 2010 Kamioka Y., et al. Multiple Decisive Phosphorylation Sites for the Negative Feedback Regulation of SOS1 via ERK. 4 J Biol Chem 285 (37) 28826 2010 Hoshino A., et al. De�ciency of Chemokine Receptor CCR1 Causes Osteopenia Due to Impaired Functions of Osteoclasts and Osteoblasts. 5 Tissue Engineering Part C 16 (2) 311 2010 Evenou F., et al. Spontaneous Formation of Highly Functional Three-Dimensional Multilayer from Human Hepatoma Hep G2 Cells Cultured on an Oxygen-Permeable Polydimethylsiloxane Membrane. 6 J Biol Chem 285 (36) 28286 2010 Fujihara C., et al. Role of Mechanical Stress-induced Glutamate Signaling-associated Molecules in Cytodifferentiation of Periodontal Ligament Cells. 7 J Biol Chem 285 (31) 23581 2010 Okada S., et al. Bach1-dependent and -independent Regulation of Heme Oxygenase-1 in Keratinocytes. 8 J Biol Chem 285 (27) 21013 2010 Nagano M., et al. ZF21 Protein Regulates Cell Adhesion and Motility. 9 J Biol Chem 285 (10) 7818 2010 Matsunaga-U. R., et al. The Scaffold Protein Shoc2/SUR-8 Accelerates the Interaction of Ras and Raf. 10 J Invest Dermatol 131 (1) 37 2010 Taguchi S., et al. Overexpression of the Transcription Factor Yin-Yang-1 Suppresses Differentiation of HaCaT Cells in Three-Dimensional Cell Culture.

Journal Vol (No) Page Year Author(s) Title 1 PloS one 14 (3) 2019 Matsumoto E., et al. Fabricating retinal pigment epithelial cell sheets derived from human induced pluripotent stem cells in an automated closed culture system for regenerative medicine 2 Scienti�c Reports 8 6248 2018 Wakuda Y., et al Native collagen hydrogel nano�bres with anisotropic structure using core-shell electrospinning. 3 Tissue Eng Part C Methods 24 (2) 108 2018 Zhu X., et al. A Miniature Swine Model for Stem Cell-Based De Novo Regeneration of Dental Pulp and Dentin-Like Tissue. 4 Oral Diseases 23 620 2017 Nakayama H., et al. Enhanced regeneration potential of mobilized dental pulp stem cells from immature teeth. 5 Stem Cell Res Ther. 7 (77) 2016 Kawamura R., et al. EDTA soluble chemical components and the conditioned medium from mobilized dental pulp stem cells contain an inductive microenvironment, promoting cell proliferation, migration, and odontoblastic differentiation. 6 Oral Diseases 21 113 2015 Takeuchi N., et al. Similar in vitro effects and pulp regeneration in ectopic tooth transplantation by basic �broblast growth factor and granulocyte-colony stimulating factor. 7 Stem Cell Res Ther. 6 (111) 2015 Hayashi Y., et al. CXCL14 and MCP1 are potent trophic factors associated with cell migration and angiogenesis leading to higher regenerative potential of dental pulp side population cells. 8 International Endodontic Journal 47 713 2014 Lin LM., et al. Regeneration of the dentine–pulp complex with revitalization/revascularization therapy: challenges and hopes. 9 ProS one 9 (5) e98553 2014 Horibe H., et al. Isolation of a Stable Subpopulation of Mobilized Dental Pulp Stem Cells (MDPSCs) with High Proliferation, Migration, and Regeneration Potential Is Independent of Age. 10 Biomaterials 34 1888 2013 Ishizaka R., et al. Stimulation of angiogenesis, neurogenesis and regeneration by side population cells from dental pulp. 11 Biomaterials 34 9036 2013 Murakami M., et al. The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential. 12 Biomaterials 33 2109 2012 Ishizaka R., et al. Regeneration of dental pulp following pulpectomy by fractionated stem/progenitor cells from bone marrow and adipose tissue. 13 J Endod. 38 (7) 920 2012 Murakami M., et al. Identi�cation of Novel Function of Vimentin for Quality Standard for Regenerated Pulp Tissue. 14 Tissue Engineering: Part A 17 (15-16) 1911 2011 Iohara K., et al. Complete Pulp Regeneration After Pulpectomy by Transplantation of CD105+ Stem Cells with Stromal Cell-Derived Factor-1.

Collected Papers for “beMatrix Collagen”

Journal Vol (No) Page Year Author(s) Title 1 Surgery Today 49 785 2019 Yamashita K., et al Intraperitoneal chemotherapy for peritoneal metastases using sustained release formula of cisplatin-incorporated gelatin hydrogel granules 2 The AAPS Journal 20 (6) 105 2018 Zhou J., et al Reverse Engineering the 1-Month Lupron Depot®. 3 Cell Stem Cell 22 128 2018 Jinnou H., et al. Radial Glial Fibers Promote Neuronal Migration and Functional Recovery after Neonatal Brain Injury. 4 Advanced Healthcare Materials 6 1700183 2017 Oshikawa M., et al. Af�nity-Immobilization of VEGF on Laminin Porous Sponge Enhances Angiogenesis in the Ischemic Brain. 5 J Biol Macromol 91 789 2016 Horinaka J., et al. Rheological properties of concentrated solutions of gelatin in an ionic liquid 1-ethyl-3-methylimidazolium dimethyl phosphate. 6 Adv Healthc Mater 5 (5) 541 2016 Koshy ST., et al. Click-Crosslinked Injectable Gelatin Hydrogels. 7 Heart and Vessels 31 5 2016 Kumagai M., et al. Safety and ef�cacy of sustained release of basic �broblast growth factor using gelatin hydrogel in patients with critical limb ischemia. 8 J Periodontal Res 5 (1) 77 2016 Hoshi S., et al. Ridge augmentation using recombinant human �broblast growth factor-2 with biodegradable gelatin sponges incorporating β-tricalcium phosphate: a preclinical study in dogs. 9 Biomaterilas 63 14 2015 Yoshizawa K., et al. Enhanced angiogenesis of growth factor-free porous biodegradable adhesive made with hexanoyl group-modi�ed gelatin. 10 BMJ Open 5 e007733 2015 Morimoto N., et al. Exploratory clinical trial of combination wound therapy with a gelatin sheet and platelet-rich plasma in patients with chronic skin ulcers: study protocol. 11 J Pediatr Surg 50 (2) 255 2015 Ishimaru T., et al. Slow release of basic �broblast growth factor (b-FGF) enhances mechanical properties of rat trachea. 12 Tissue Eng Part A 21 (1-2) 193 2015 Ajioka I., et al. Enhancement of neuroblast migration into the injured cerebral cortex using laminin-containing porous sponge. 13 Tissue Eng Part A 21 (13-14) 2025 2015 Kabuto Y., et al. Stimulation of Rotator Cuff Repair by Sustained Release of Bone Morphogenetic Protein-7 Using a Gelatin Hydrogel Sheet. 14 Invest Ophthalmol Vis Sci 55 (4) 2337 2014 Kimoto M., et al. Development of a Bioengineered Corneal Endothelial Cell Sheet to Fit the Corneal Curvature. 15 J Bioact Compat Polym 29 (6) 560 2014 Yoshizawa K., et al. Bonding behavior of hydrophobically modi�ed gelatin �lms on the intestinal surface. 16 J Biomater Appl 28 (6) 880 2014 Inoue M., et al. Effects of ultraviolet irradiation on bonding strength between Co-Cr alloy and citric acid-crosslinked gelatin matrix. 17 J Biosci Bioeng 118 (1) 112 2014 Ohyabu Y., et al. Evaluation of gelatin hydrogel as a potential carrier for cell transportation. 18 Int J Mol Sci 15 (2) 2142 2014 Yoshizawa K., et al. Enhanced Bonding Strength of Hydrophobically Modi�ed Gelatin Films on Wet Blood Vessels. 19 Key Eng Mater 631 397 2014 Kiminami K., et al. Development of Bioresorbable Calcium-Phosphate Cements Hybridized with Gelatin Particles and their In Vivo Evaluation Using Pig’s Tibia Model. 20 J Biomed Mater Res A 101A (7) 2049 2013 Inoue M., et al. Poly-(L-lactic acid) and citric acid-crosslinked gelatin composite matrices as a drug-eluting stent coating material with endothelialization, antithrombogenic, and drug release properties. 21 Acta Biomater 8 (5) 1792 2012 Matsui M., et al. Enhanced angiogenesis by multiple release of platelet-rich plasma contents and basic �broblast growth factor from gelatin hydrogels. 22 Adv Healthc Mater 1 (5) 573 2012 Inoue M., et al. An Antithrombogenic Citric Acid-Crosslinked Gelatin with Endothelialization Activity. 23 Sci Technol Adv Mater 13 (6) 064215 2012 Inoue M., et al. Biodegradable organic acid-crosslinked alkali-treated gelatins with anti-thrombogenic and endothelialization properties. 24 Colloids Surf B Biointerfaces 88 (1) 260 2011 Inoue M., et al. UV irradiation enhances the bonding strength between citric acid-crosslinked gelatin and stainless steel. 25 Geriatr Gerontol Int 11 527 2011 Tara S., et al. Novel approach to ischemic skin ulcer in systemic lupus erythematosus: Therapeutic angiogenesis by controlled?release basic �broblast growth factor. 26 BMC Medicine 8 76 2010 Nakagawa T., et al. Topical insulin-like growth factor 1 treatment using gelatin hydrogels for glucocorticoid-resistant sudden sensorineural hearing loss: a prospective clinical trial. 27 Am J Med Sci 338 (4) 341 2009 Kawanaka H., et al. Therapeutic Angiogenesis by Controlled-Release Fibroblast Growth Factor in a Patient With Churg-Strauss Syndrome Complicated by an Intractable Ischemic Leg Ulcer.

Collected Papers for “beMatrix Gelatin”

Research & Development CenterBiomedical Department

2-22 Futamata, Yao-shi, Osaka 58l-0024 JAPANTEL: +81-72-949-8702

E-mail: [email protected]

● Unauthorized copying, reproduction, and replication of the contents of this pamphlet are prohibited. The appearance of products and prices are subject to change without notice. Published in Apr. 2020

Documents

Collagen and Gelatin Product Guide Feb. 2020 › en › biomedical › ... · Collagen for Cell Culture Collagen solution at pH 3 with a concentration of 3 mg/mL Storage condition: