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Clinical: Venepuncture SOP
Document Control Summary
Status: New
Version: e.g. v1.0 Date: June 2017
Author/Owner/Title: Di Hughes
Approved by: Policy and Procedures Committee Date: 20 July 2017
Ratified: Policy and Procedures Committee Date: 20 July 2017
Related Trust Strategy and/or Strategic Aims
Physical health Pathway Physical Health CQUIN
Implementation Date: August 2017
Review Date: June 2020
Key Words: Venepuncture, blood taking, blood samples, phlebotomy, bloods
Associated Policy or Standard Operating Procedures
Physical Health
Change Control – Amendment History
Version Dates Amendments
Venepuncture SOP June 2017
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Contents
1. Introduction ..................................................................................................................... 3
2. Purpose ............................................................................................................................ 3
3. Scope ............................................................................................................................... 3
4. Precautions....................................................................................................................... 4
5. Exclusion Criteria………………………………………………………………………………………………………………4
6. Equipment ........................................................................................................................ 5
7. Procedure…………………………………………………………………………………………………………………………6
8. Complications ................................................................................................................. 11
9. Process For Monitoring Compliance And Effectiveness ..................................................... 12
10. References/Bibliography ................................................................................................. 13
Appendix 1. Order of draw for multiple blood samples……………………………………………………………….14
Appendix 2. Veins to consider for venepuncture…………………………………………………………………………15
Appendix 3. Clinical Skills Competency Assessment / Re-assessment for:
Peripheral Venepuncture……………………………………………………………………………………….16
Venepuncture SOP June 2017
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1. Introduction
Venepuncture is a procedure that involves entering a vein with a needle in order to obtain a
venous blood sample for haematological, biochemical or bacteriological analysis where the
clinical investigation has been requested by the Clinician or it is in the best interest for the
client to receive treatment in line with the service users care plan.
This SOP should be read in conjunction with Infection Control policies/SOPs regarding hand
washing, disposal of sharps, needle stick injuries and universal precautions
2. Purpose
This SOP sets out the training and competency requirements for all staff to undertake
venepuncture.
It also covers the full procedure to be followed, including risks and contraindications.
It does not cover interpretation of blood results.
3. Scope
This SOP applies to any member of staff who wishes to undertake venepuncture. All staff
grades are eligible to undertake training to enable them to practice venepuncture, at the
discretion of their manager, dependant on the needs of the service where that staff member
works.
Staff undertaking venepuncture will be equipped with the knowledge and skills required to
undertake this procedure safely and appropriately, through attending relevant, approved,
theoretical and practical training.
Managers must ensure that members of staff intending to perform venepuncture have
completed such training and attained the requisite current competences before undertaking
such duties.
Any non-medical staff required to obtain samples in this way will require documented
supervised practice following training. If a new member of staff has practiced venepuncture
in a previous role and has documented evidence of formal training received, they may
practice venepuncture within this trust following supervised practice and being signed off as
competent.
All staff undertaking venepuncture will be responsible for:
ensuring that their practice will promote and protect the interests and dignity of
patients, irrespective of gender, age, race, sexuality, ability, economic status, culture
and religious or political beliefs
maintain competency and confidence
a contemporaneous knowledge and understanding of their legal, ethical and
organisational responsibilities in relation to venepuncture
where there has been a significant break in the individuals practice then documented
supervised practice and competency update should be completed
obtaining informed consent
being able to provide research based explanations for their practice
Venepuncture SOP June 2017
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recognising and acting on their personal limitations
seek appropriate assistance if they make two consecutive unsuccessful attempts
desist, report and record if at any time there is risk of harm or injury
Approved training minimum standards
Formal training session
Completion of a minimum of two successful supervised practices
Competence signed off by a competent practitioner who is able to assess
competence. This assessor must be competent in venepuncture themselves, but
does not necessarily need to be a doctor or a qualified nurse.
A copy of this signed competency document must be given to the line manager to be
held on the individual’s personal file
No update of theoretical training required, but competency must be reassessed
annually, or following any extended period of non-practice
4. Precautions Standard precautions must be observed at all times, including hand washing and
decontamination
All items used must be sterile single use
All sharps used must be safety devices
A closed vaccumised blood collection system must be used, e.g. Vacutainer
It is not acceptable to use a needle and syringe for obtaining blood samples, as this
not only increases the risk of needle stick injuries, but it also compromises the
integrity of the blood sample by increased risk of contamination and increased risk of
damage to blood cells, leading to a haemolysed sample, which cannot be used
Aseptic non-touch technique (ANTT) should be used
Personal protective equipment must be used, including non-sterile latex-free gloves
and aprons. Safety goggles or a face shield should be considered if the patient is
known to have a blood borne virus and/or is likely to do anything that may increase
risk of blood splatter
Skin must be cleansed using the recommended swab (Chloraprep®) and allowed to
air dry
5. Exclusion Criteria
Do not take blood from:
Limbs with arterial-venous fistula
Contraindicated limbs, e.g. post mastectomy surgery or affected by a stroke
Limbs affected by severe disabling conditions such as rheumatoid arthritis
Veins that are damaged or traumatised, bruised, inflamed, fibrosed or fragile
Veins that are adjacent to infection or phlebitis
Veins close to, or that have, existing or recent intravenous or subcutaneous
cannulation
Anyone on whom you have failed to obtain a sample on two consecutive attempts –
in this case you should refer to another practitioner
Venepuncture SOP June 2017
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Intravenous infusions:
Wherever possible, blood should not be taken from the same limb where an intravenous
infusion is running. Where this is absolutely necessary, an appropriate clinician must be
informed and infusion stopped by them at least 30 minutes prior to blood sampling, and
restarted immediately after
6. Equipment Ensure all equipment is available before commencing procedure. If possible, position sharps
container and blood collection system nearest to your dominant hand, and position all
necessary blood bottles and swabs nearest to your non-dominant hand. This will help
procedure to be carried out more smoothly and efficiently.
Equipment required:
Plastic apron
Non-sterile latex-free gloves of an appropriate size (well-fitting gloves will make
palpating the vein much easier)
Sharps container
Single use disposable tourniquet
Chloraprep® skin preparation
Appropriate safety device needle or butterfly with vacutainer blood collection system
attached
All required blood bottles (please see Appendix 1 “order of draw”)
Lint-free swabs
Plaster and/or surgical tape
Venepuncture SOP June 2017
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7. Procedure
Action Rationale 1 Approach the patient in a confident manner
and explain and discuss the procedure with
them
To ensure that the patient understands the
procedure and gives their valid consent
(NMC 2015)
2 Allow patient to ask questions and discuss
any concerns they may have.
Check previous history to identify any history
of stroke or mastectomy or any previous
difficulties with venous access
Anxiety results in vasoconstriction, therefore
a patient who is relaxed will have more
dilated veins and access will be easier.
Previous history may influence limb or vein
selection
3 Check that the request form is correct, fully
completed and signed
Blood samples must not be taken unless the
responsible clinician has correctly completed
a request form for the appropriate lab, clearly
identifying full patient details and all tests
required
4 Check the identity of the patient matches
the details on the request form by asking for
their full name and date of birth
To ensure that a) the request form has been
completed correctly and b) that the sample is
taken from the correct patient
5 Checks hands for any visibly broken skin,
cover with waterproof dressing
To minimise the risk of contamination to the
practitioner
6 Assemble equipment required for the
procedure (see list above)
To ensure that the procedure goes as
smoothly as possible without interruption
7 Carefully wash hands using trust approved
hand washing procedure and dry thoroughly
To minimise the risk of infection (DoH 2010)
8 Check all packaging for any damage and
expiry dates before opening and preparing
equipment on chosen clean surface
To maintain asepsis throughout and ensure
no faulty equipment used
9 Take all equipment to the patient,
exhibiting a relaxed and confident manner
To help the patient feel at ease
10 Support the chosen limb in a comfortable
and extended position
To ensure patient comfort and facilitate
venous access
Venepuncture SOP June 2017
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11 If possible, palpate and choose the vein
without the use of a tourniquet, if not then
apply single use tourniquet to selected limb,
approx. 4 fingers above target area. It should
be tight enough to make inserting 2 fingers
under it difficult, but not tight enough to
occlude the pulse. If palpation of the vein is
difficult try
a) Allowing patient to warm up if cold
b) Ask patient to make a fist – but not to
clench and unclench, as this
adversely affects the blood results
c) Gently stroke veins in a downward
motion – DO NOT TAP
d) Allow limb to hang down
Reusable tourniquets not to be used under
any circumstance, due to infection control.
To increase prominence of the vein without
disrupting circulation
To promote blood flow and therefore distend
the veins
Allowing gravity to assist distention of the
veins
12 Select the vein by observation and careful
palpation to determine size, depth and
condition. Whilst the antecubital fossa is the
most common site, it is also acceptable to
use forearm, hand or foot if preferable or
necessary
To prevent inadvertent insertion of needle
into other anatomical structure (e.g. artery)
Venepuncture SOP June 2017
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13 Release the tourniquet until ready to
actually enter the vein
To ensure the maximum time for tourniquet
application, one minute, is not exceeded
14 Select device to be used, based on vein
size, site and volume of blood to be taken
Standard vacutainer needle is usually first
choice if using antecubital fossa, but if using
any other site, e.g. lower arm, hand or foot, a
23 gauge winged infusion device (blue
butterfly) should be utilised
To reduce risk of damage/trauma to the vein
and therefore prevent haemolysis
15 Decontaminate hands using alcohol foam To maintain asepsis and minimize the risk of
infection/cross-infection
16 Clean patients skin around selected site
using the chloraprep® swab. Squeeze
between finger and thumb to break internal
seal, press against patients skin a couple of
times to release liquid flow, rub over selected
area for approximately 30 seconds
Allow to dry for at least 30 seconds.
Do not re-palpate vein or touch the skin
To maintain asepsis and minimize the risk of
infection
To prevent pain on insertion and reduce risk
of contamination of blood sample
To maintain asepsis
17 Put on well-fitting non-sterile gloves To prevent cross-infection
18 Reapply tourniquet as before, ensure time
is noted to monitor length of time tourniquet
is used
To dilate veins by obstructing the venous
return
19 Remove cover from needle and inspect
carefully
To detect faulty equipment, e.g. bent or
barbed needle
Venepuncture SOP June 2017
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20 Anchor the vein by applying slight manual
traction on the skin a couple of centimetres
below the proposed insertion site
Immobilises the vein by providing counter
tension. This will facilitate smoother needle
entry
21 Holding needle with bevel uppermost,
insert needle smoothly in one fluid
movement, at an angle of approx. 15-30º,
dependant on depth and size of vein
To facilitate a successful, pain-free
venepuncture
22 If you have missed the vein, do not sweep
the needle around in search of the vein,
gently withdraw until the tip is just below the
skin, adjust angle and try again
To prevent causing unnecessary pain and
damage
23 Hold the needle still and support with
fingers/thumb and do not exert any pressure
on the needle
To avoid puncturing through the back of the
vein wall
24 Following the correct “Order of Draw”
(Appendix 1), insert first blood bottle into
vacutainer holder, being sure to support the
holder to prevent applying pressure to the
needle
To Minimize the risk of transference of
additives from one bottle to another,
therefore potentially contaminating the
sample
25 Release tourniquet To decrease the pressure in the vein and
decrease risk of haemolysis and ensure the
maximum one minute is not exceeded
Venepuncture SOP June 2017
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26 Disconnect bottle from holder and insert
next one required. Remember to invert each
bottle several times – DO NOT shake
To complete requested tests
To ensure even distribution of additives
whilst preventing haemolysis
27 Select a cotton wool ball or swab and
position above needle entry point without
touching
DO NOT apply pressure until needle is totally
out
Be ready to apply pressure once needle
removed
To prevent unnecessary pain and potential
damage to the intima of the vein
28 Remove the needle in a single smooth
movement, activate safety device and
dispose in sharps container
Reduces pain to patient and risk of sharps
injury to practitioner
29 Immediately needle is out of skin, apply
digital pressure for at least one minute, or
until bleeding has stopped (whichever is
longer). You may ask the patient to apply
pressure themselves, whilst you deal with
the samples, but discourage them from
bending the elbow, as this causes bruising
If haematoma noted, apply firm pressure for
at least 5 minutes
To prevent leakage and haematoma
formation, thereby preserving integrity of
vein for possible future use
To reduce bleeding and bruising
30 Fully label bottles before leaving the
patient’s side
To ensure no mistakes are made with mixing
up samples from different patients
31 Inspect puncture site and if happy, apply
either a plaster, or a clean swab and surgical
tape if patient is sensitive to plasters. Ideally,
this should stay in place for 1-2 hours, but
may be removed after 15 minutes
To ensure that the puncture site has sealed
and to cover to prevent possible leakage or
contamination
32 Ensure patient is comfortable and not
feeling faint or unwell in any way
To ascertain if it is safe for patient to leave
the treatment room, or whether further
measures need to be taken
33 Remove protective equipment and
dispose of all waste according to trust policy
To ensure safe disposal of sharps and other
equipment
34 Ensure samples are correctly stored with
the appropriate form and follow local
procedure for collection/transportation of
specimens
To ensure all samples reach their intended
destination safely
35 Document procedure in patient notes,
according to local procedures
To ensure and maintain timely and accurate
record keeping
Venepuncture SOP June 2017
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8. Complications There are a number of possible problems associated with practice of venepuncture. The
prevention and treatment of potential complications are outlined in more detail in The Manual
of Clinical Nursing Procedures (2015).
Some main complications are detailed below:
Problem Possible Cause Action
Pain Touched a nerve Punctured an artery Anxiety Use of vein in sensitive area, e.g. wrist/back of hand
Remove needle Remove needle/apply pressure Reassure and calm patient Try alternative site if possible
Anxiety Fear of needles Previous bad experience General state of mind
Give patient time to ask questions and feel comfortable Offer continuous reassurance
Limited venous access Repeated use of same veins Previous poor technique, choice of vein or device Peripheral shutdown Dehydration
Look at alternative sites Consider use of smaller butterfly device Consider trying to warm skin Making patient drink will not make this better Consider referring to someone with more experience
Bruising and/or
haematoma
Needle punctured posterior wall of the vein Inadequate pressure on removal of needle Failure to remove tourniquet before removing needle Poor technique/bad choice of vein or device
Ensure correct technique and choice of vein and device used at all times If bleeding or haematoma occurs, apply pressure for at least 5 minutes and keep patient still and calm
Infection at venepuncture
site
Poor aseptic technique Refer for medical treatment – ensure good asepsis maintained in future
Vasovagal syncope (faint) Fear of needles Sight of blood Pain Extremely hot environment Extreme emotional distress
Occurs when body overreacts to certain triggers, it causes heart rate and blood pressure to drop suddenly, causing reduced blood flow to brain, producing the faint Usually does not require treatment other than keep patient lying down until they feel better, release tight clothing and cool the environment if possible
Venepuncture SOP June 2017
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Needle stick injury to
practitioner
Unsafe practice Incorrect disposal of sharps
Follow trust policy regarding needle stick injury
Accidental blood spillage Poor technique Faulty equipment
Follow trust policy for clearing up blood spills Complete incident form and report faulty device
Missed vein Poor vein selection Inadequate anchoring of vein Wrong positioning Lack of light Poor concentration Difficult access
Correct issues that can be corrected regarding light, technique, concentration etc. Try one more time, if still unable to get access then refer to another practitioner
Spurt of blood on entry Bevel tip of needle enters vein before entire bevel is under the skin Tourniquet is far too tight
Angle of entry is too steep, adjust angle of entry to between 15 and 30º Check and adjust tourniquet, you should still be able to palpate the pulse
Blood stops flowing Needle has been advanced through the back of the vein Bevel may be lying against vein wall or valve The vein has collapsed due to device being too big for vein or vein was too tiny
Gently withdraw about 1mm, if flow does not return then remove needle and apply pressure Remove needle, apply pressure, consider choosing a larger vein and/or smaller device before attempting again
9. Process For Monitoring Compliance And Effectiveness
Patient feedback and incident reports will be monitored and reviewed
Training and competence will be recorded on ESR and in staff members’ personal files
Venepuncture SOP June 2017
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10. References/Bibliography
Campbell, H., Carrington, M. and Limber, C. (1999) A Practice Guide to Venepuncture and
Management of Complications. British Journal of Nursing, 8(7), 426-431
Dougherty, L. and Lamb, J. (Eds) (2008) Intravenous Therapy in Nursing practice, 2nd ed.
Oxford: Blackwell
Hoke, R.F. (2015) Phlebotomy. London: National Association of Phlebotomists
Mallet, J. and Dougherty, L. (Eds) (2015) The Royal Marsden Manual of Clinical Nursing
Procedures 9th ed. London: Blackwell Scientific Publications
NMC (2015) The Code: Standard of Conduct, Performance and Ethics for Nurses and
Midwives. London: Nursing and Midwifery Council
McCall, R.E. and Tankersley, C.M. (2012) Phlebotomy Essentials 5th ed. Philadelphia:
Lippincote Williams and Wilkins
RCN (2010) Standards for Infusion Therapy 3rd ed. London: Royal College of Nursing
WHO (2010) WHO Guidelines on Drawing Blood: best practices in phlebotomy.
Geneva: World Health Organization Press
Venepuncture SOP June 2017
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Appendix One
ORDER OF DRAW FOR MULTIPLE BLOOD SAMPLES
Venepuncture SOP June 2017
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Appendix Two
VEINS TO CONSIDER FOR VENEPUNCTURE
ARM
HAND
Page 16 of 17
Appendix Three
Clinical Skills Competency Assessment/Re-Assessment for: Peripheral Venepuncture Candidates must be assessed a minimum of twice before completion of the final declaration on the last page
Correctly demonstrate the following: Date Date Date Date
Assessors Name and Initials
Assessors Name and Initials
Assessors Name and Initials
Assessors Name and Initials
Preparation of equipment for venepuncture. Assembles appropriate equipment on a clean tray. Needles (must be safety devices), sharps bin, disposable tourniquet, gloves, apron, skin disinfectant. Prepares tray and equipment using ANTT. Collects appropriate blood bottles. Ensures all equipment in date and sterile. Has correct blood forms for patient
Prepares patient for Venepuncture. Correctly identifies patient. Appropriate consent obtained. Explanation of procedure given.
Prepares self for procedure. Hand washing performed as trust policy and alcohol hand rub applied. Non sterile gloves and apron worn. Ensures appropriate environment. Well lit, ventilated, flooring is as clinical area.
Selects appropriate site for venepuncture. Identifies most suitable vein for sampling. Applies tourniquet 5-10cms from the intended puncture site applying enough pressure to obstruct venous but not arterial flow. Ensures site for sampling is warm and well perfused. Cleanses skin according to SOP
Demonstrates needle insertion Immobilises limb and applies traction to skin in order to anchor the vein maintaining asepsis. With bevel uppermost inserts needle at an angle of about 30 degrees depending on depth of vein. Successfully obtains blood sample / acts appropriately in the event of non-success
Demonstrates safe removal of needle. Releases tourniquet, occludes the vein and removes needle, applying direct pressure for approximately 1 minute Disposes of needle and syringe in appropriate sharps bin. Applies appropriate dressing
Demonstrates appropriate documentation
Puts blood in correct specimen bottles in correct order to prevent contamination. Labels blood bottles correctly and sends to lab safely. Documents procedure on Clinical system (e.g. RiO/Lily )
Page 17 of 17
Declaration of Competence to Practice Venepuncture Independently
Practitioners name:……………………………………………………
Once both assessor and practitioner are satisfied that competence has been achieved, both must
sign below and a copy given to the individual’s manager for insertion in the personal file.
Assessor’s Declaration
I confirm that I have completed a clinical workplace assessment and can confirm that the above
named practitioner is competent at carrying out the venepuncture procedure
Name:……………………………………………….. Job Title:……..………………………………...
Signature:……………………………………………Date:…………………………………………….
Practitioner’s Self Declaration
I confirm that I am competent to practice venepuncture and understand that I am
responsible and accountable for my professional practice.
I have completed the theoretical venepuncture training and have undertaken
supervised practice for venepuncture in the clinical setting
I understand that this is an invasive procedure that requires frequent practice and that I must have
my competence re-assessed every year.
I am responsible and accountable for keeping my practice up-to date and in line with Trust and
national polices.
Name:……………………………………………….. Job Title:……..………………………………...
Signature:……………………………………………Date:…………………………………………….