CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY BSL4 …httpAssets... · CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY BSL4 FACILITY L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY)

Meeting of Experts - Palais des Nations

Geneva 4 - 8 August 2014

CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY

BSL4 FACILITY

L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY)

Prof. Maria Rita Gismondo

http://www.google.com/url?url=http://www.clinicamartin.it/le-10-linee-guida-del-ministero-della-salute/&rct=j&frm=1&q=&esrc=s&sa=U&ei=d5PHU7WsL6b54QS09oD4Bw&ved=0CBYQ9QEwAA&usg=AFQjCNETXJ6cbyMhOqJKSgAaD2LWStQCZQ




BSL4 FACILITY



RESEARCH ACTIVITIES

Antibiotic Resistance, Antiviral

Resistance, New Tropical

Diseases, Multiresistan

Mycobacteria etc…

Results are published in open

scientific literature





BSL4 FACILITY



INTERNATIONAL PARTNERSHIPS AND PROJECTS

Capacity – Building

Gap Analysis

Training (E-learning / In-presence) and Sharing Experience

Hands-on courses (BSL2; BSL3 and BSL4) tailored to countries local needs

Workshops and seminars





BSL4 FACILITY



INTERNATIONAL PARTNERSHIPS

AND PROJECTS – ONGOING

1.The Master Euro-Africain: Biosécurité et

Biosûreté

2.Biorisk Assessment Mission, Tiblisi

3.CBRN CoE Initiative (P3, P6, P7, P13 and

P18)

4.Instrument for Stability





BSL4 FACILITY



Georgia, Tbilisi





BSL4 FACILITY



International conference “Biosecurity and Biosafety: future trends and solutions”





BSL4 FACILITY



Develop a holistic and multidisciplinary approach on bio-related issues:

bio-threats analysis, biosafety, biosecurity, biorisk assessment and management and

biohazardous waste containment

Support and foster a risk mitigation culture

Support the harmonization of international bio-safety and bio-security standards among

the participating countries

Strengthen regional and international collaboration and cooperation so as knowledge

sharing and lessons learned exchange

Enhance networking and awareness raising on bio-related issues and and support

synergies among scientific communities and institutions.





BSL4 FACILITY



Prof. Maria Rita Gismondo, MD

[email protected] Address: Via G. B. Grassi, 74, 20157 Milan

(Italy)

Phone: +39 02 39042239 / +39 02

50319831

Fax: +39 02 50319832

Dott. Francesca Cerutti

[email protected]


mailto:[email protected]

“TRAINING ON FIELD”

A practical training for the isolation of

Bacillus anthracis from environmental samples in

high-risk areas

Meeting of Experts

Palais des Nations, Geneva

4 - 8 August 2014.

Antonio Fasanella

Istituto Zooprofilattico Sperimentale di Puglia e Basilicata

Foggia, Italy


NETWORK OF ISTITUTI ZOOPROFILATTICI

SPERIMENTALI


• Control of zoonosis

• Food security programs

• Management of emergency ( brucellosis, tuberculosis,

avian flu, anthrax, blue tongue )

• Research on animal infectious diseases

• Development of vaccines for veterinary use

• Cooperation with national and international scientific

organizations


Anthrax Reference

Institute of Italy


Major tasks of the

Anthrax Reference Institute of Italy

• Anthrax vaccine production

• Molecular epidemiology and genotype database

• Disease control

• Bioterrorism emergency ( Italian Laboratory Reference)

• Animal model

• Research


Anthrax in Italy

The low diversity of B. anthracis genotypes in

Italy suggests a single, dominant historical

introduction, followed by localized

differentiation

In Italy, anthrax is a sporadic

disease. Every year, few

outbreaks occur in the known

risk areas of central-southern

Italy and in the major islands.


Pollino National Park ( Basilicata

Region – South Italy) epidemic map,

with the representation of the single

outbreaks identified by GIS

information. Foggia

During the 2004 summer in the

Pollino National Park in

Basilicata it was registered an

anthrax epidemic that involved

41 farms of 13 towns in an area

of about 900 Km2 in which there

were about 7.000 cattle and

33.000 sheep and goats.

In 4 weeks 81 cattle died, as well as 15 sheep, 9 goats,11 horses and 8 deer.

Epidemic outbreak of 2004


Bovine

Carcass burial Horse

Red Deer



More than 17% of the population of deer in the park died during the outbreak of

anthrax

(Fasanella A. et al. 2007 Anthrax in Red Deer (Cervus elaphus), Italy. Emerg Infect Dis. 13(7): 1118–1119)




Two human suspect cases


Outdoor laboratory

In some restricted areas of the park, for several time were buried animals died of anthrax

Vaccination programs ensure the control of the

disease in animals that graze in that area.

These areas represent a valuable environment for the study of the ecology of

Bacillus anthracis


Since 2010, the Anthrax Reference

Institute of Italy use this outdoor

laboratory for trainings direct to

researchers (from foreign countries)

regarding the technique of isolation of

spores from contaminated environments.

Outdoor laboratory

These training activities have been

continued in the framework of the

subsequent Joint Action QUANDHIP. For

further information on QUANDHIP, please,

see website on next slide.

The “training on field” was included in

the EU funded project EQADeBa, as an

educational activity.


http://www.quandhip.eu/

http://www.quandhip.eu/

Training on field: Program

• •First day: – Arrival at the airport of Bari and departure to Pollino National Park . Overnight

• •Second day: – Sampling of soils in areas of National Park. Return to Foggia and overnight

• •Third day: – Processing of collected samples at the BL3 laboratory of the National Reference Centre

for anthrax. Isolation of Bacillus anthracis using GABRI or WHO method

• •Fourth day: – Biomolecular analysis of suspect colonies and final meeting

• • Fifth day: – Departure from Bari airport


Training on field

Processing of collected samples


Training on field

Biomolecular analysis of suspect colonies: reading of plates


List of Institutes that participated in the training

1. Österreichische Agentur für Gesundheit und Ernährungssicherheit (AGES) –

Austria

2. Friedrich-Loeffler-Institut / Bundesforschungsinstitut für Tiergesundheit (FLI) –

Germany

3. FoHM: Folkhälsomyndigheten – Public Healt Agency of Sweden – Sweden

4. Public Healt England – England

5. Polish National Institute of Hygiene (PZH) – Poland

6. Infectiology Centre of Latvia (LIC) – Lithuania

7. Federal Office for Civil Protection (FOCP), Spiez Laboratory – Switzerland

8. Robert Koch Institute – Germany

9. Basurto University Hospital, Bilbao – Spain

10. ANSES – France

11. Faculty of Veterinary Medicine of Tirana – Albania

12. Faculty of Medicine, Mu’tah University, Al-karak – Jordan

13. Biotechnology Institute of a National Academy of Sciences of the Kyrgyz

Republic - Kyrgyzstan


Positive contribution

– Many researchers have the opportunity to observe B. anthracis in its natural environment

– Exchange of opinions and experiences

– Incentive to improve the technique of isolation

– Start of new collaborations

• The aim of the training is to prepare the researchers in the techniques of collection of contaminated soil samples.

• This training aims to verify the contamination level of environment (natural or with suspect of a deliberate release).

• The training is free and is directed to researchers prior approval of the Italian government authorities


Contacts:

Antonio FASANELLA

[email protected]

Angela ACETI

[email protected]

Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata

WEB SITE: www.izsfg.it



http://www.izsfg.it/


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CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY BSL4 …httpAssets... · CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY BSL4 FACILITY L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY)