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8/16/2019 Clinical Chemistry 1
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Part II: Course Syllabus
Table of Contents
Item Page
Instructor Information 2-1
Course Information2-2
Course Objectives2-4
Learning Resources2-22
Course Requirements & Grading 2-28
2 - 0
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Instructor Information
DR. ESSAM HUSAIN JIFFRI (Course Co-ordinator) Tee!"one# $%&e# '0000 E*. 2+,
Moie# 00'+/E-ai# e1iri34aau.edu.sa
$%&e 5o&ation# st 6oor7 Roo No. 28 $%&e Hour# Sat# 0#00 9 2#00 :M ;ed#0#00 9 2#00 :M
R! "#$% "O'(#- Tee!"one# $%&e# '0000 E*. 220,/ 82020/
Moie# 00'02, - E-ai# "4"o1a34aau.edu.sa - $%&e 5o&ation# 2nd 6oor7 Roo No. 282 - $%&e Hour# Sun /#00 9 #00 AM
Tue /#00 9 #00 AM
DR. ADEE5
8/16/2019 Clinical Chemistry 1
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CO'R)% *#$%# Cini&a C"eistr@-
CO'R)% *'$+%R# M5T +0
CO'R)% $%%,I*G ,I$%)
Lecture hours: + "ours8ee4 Bor ee4s (Tota 2 "ours)
Practical hours: 2 "ours8ee4 Bor ee4s (Tota 2>"ours)
Tutorial hours: "our8ee4 Bor ee4s (Tota "ours)
LECTUREThree/Week
PRCTICLST!o/Week
TUT"RILS"ne/Week
#LE
Sat 0>#00-0/#00a.
Sat 02#0090#00 !. (
8/16/2019 Clinical Chemistry 1
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CO'R)% /%+)I,% #R%))
CO'R)% .R%R%0'I)I,%) #* R%0'IR%$%*,)
A su&&essBu &o!etion oB io&"eistr@ (M5T 20) it" a na
&uuatie rade oB aoe '0G is t"e !re-reuisite Bor Cini&a
C"eistr@- (M5T +0).
%*,R L%%L )ILL)
T"e student s"oud dis!a@ asi& readin7 ritin7 and at"eati&as4is
%)CRI.,IO* O3 ,"% CO'R)%
T"is &ourse i e a detaied stud@ oB t"e &"ei&a and
instruenta ana@ses oB "uan iooi&a ateria oB &ini&a
sini&an&e. T"e student i e introdu&ed to &oon@ used
&ini&a &"eistr@ te&"niues. T"is &ourse dis&usses t"e &ini&a
as!e&ts oB 5a Mat"7 Sour&es oB errors7 &aro"@drates7 Aino-
a&ido!at"es7 !roteins7 itains7 Tra&e Eeents7 en@es7
ee&tro@tes. T"is &ourse i interate t"e Booin to!i&s into t"e
aorator@ and e&ture sessions# aorator@ at"eati&s7 uait@
assuran&e7 s!e&ien &oe&tion and !ro&essin7 sour&es oB
iooi&a ariation7 and eauation te&"niues.
2 - +
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Course "b&ecti'es
G%*%R#L O+(%C,I%)
U!on &o!etion oB t"is &ourse t"e student i e ae to#
A&"iee t"e o1e&ties desinated @ a uet under ea&" e&ture7
rou! oB e&tures or !ra&ti&a sessions.
2 -
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O+(%C,I%) O3 L%C,'R% ,O.IC)
T"e &ourse &onsists oB e&tures7 !ra&ti&a &asses7 deonstrationsand tutorias. T"e Booin is a istin oB e&ture to!i&s and nuer
oB e&tures Bor ea&" to!i
L%C,'R% ,O.IC)*'$+%R O3L%C,'R%)
- Course S&o!e +
2- asi& K 5a Mat" +
+- Sour&e K T@!es oB Errors KLC +
- Caro"@drates '
- :roteins '
'- Tra&e Eeents +
,- itains +
>- En@oo@ '
/- Ee&tro@tes K Ca7 M7 : /
,O,#L 42
2 -
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() *SIC SC"PE +L* #T,
-Si. lectures
"b&ecti'es:
To a4e sure t"at t"e student is ae to#
. reaie7 understand and dea it" nueri&a aues in t"e
&ini&a a in t"e est !ossie a@ to re6e&t a&&ura&@ and
!re&ision oB t"e ien aue (sini&ant ures7 de&ias7
s&ienti& notation7 roundin o nuers)
2. re&onie7 understand and inter!ret SI units as a!!ies to
ass7 oue7 ent"s7 area K &on&entrations
+. &arr@ out &onersions oB sine units it"in t"e SI s@ste
. &arr@ out &onersions oB &oined units it"in t"e SI s@ste
. re&onie7 understand and inter!ret &oon@ used
&onentiona units su&" as 8d5 n857 !85
'. 4noin t"e Borua ei"t oB sustan&es7 or a!!ro!riate
&onersion Ba&tor7 e ae to &onert Bro &onentiona to SI
units.
,. 4noin t"e Borua ei"t oB sustan&es7 or a!!ro!riate
&onersion Ba&tor7 e ae to &onert Bro SI units to
&onentiona units>. &a&uate t"e &on&entration oB soutions in arious ters su&"
as 85 o857 85.
/. &a&uate oarit@ and norait@ oB soutions.
0. e*!ress t"e aount oB sustan&es in ters oB res!e&tie
!er&entaes (i.e G 8 G 87 G 8).
. or4 out sine7 uti!e7 seria and !arae diutions.
2 - '
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2. &a&uate t"e &on&entration oB ana@tes in t"e neat
sa!e "i&" as diuted @ a s!e&i& diution Ba&tor.
+. &a&uate t"e na &on&entration oB soution "i&" as
diuted @ a s!e&i& diution Ba&tor.
. or4 out "o to !re!are arious soutions Bro sto&4
soutions
. or4 out "o to !re!are arious reaents Bro arious
&o!ounds and sustan&es
'. 4noin t"e s!e&i& rait@ and G a&ids or4 out "o to
!re!are ea4er &on&entrations oB a&ids Bro &on&entrated
sto&4 soutions
,. &a&uates t"e ean7 SD K &
>. &a&uates G deiation
0) S"URCES + T1PES "$ ERR"RS
-Si. lectures
"b&ecti'es
T"e student ust e ae to
- des&rie t"e dis&i!ine oB &ini&a &"eistr@.
2- e*!ain t"e i!ortan&e oB &ini&a &"eistr@ in a edi&ine.
+- des&rie t"e Bun&tion oB t"e &ini&a &"eistr@ a.
- des&rie t"e t@!e oB resuts otained in t"e &ini&a as.
- des&rie t"e &"ara&teristi&s oB uantitatie resuts.
'- des&rie and !erBor &airation oB !i!ettes used in t"e a @
raietri& !ro&edures.
,- des&ries t"e asi& uait@ &ontro and uait@ assuran&e
reuireents Bor t"e a.
>- des&rie reiaiit@7 a&&ura&@ and !re&ision oB resuts.
2 - ,
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/- des&rie sensitiit@ and s!e&i&it@ oB !ro&edures.
0- des&rie t"e t@!es oB ana@ti&a errors in t"e a.
- des&rie t"e ee&ts oB t"ese errors on t"e resut oB tests.
2- des&rie t"e t"ree staes oB uait@ assuran&e in t"e a.
+- e*!ain t"e !reana@ti&a Ba&tors t"at a@ ead to errors in
t"e ana@sis.
- e*!ain t"e ana@ti&a Ba&tors t"at a@ ead to errors in
t"e ana@sis.
- e*!ain t"e !ostana@ti&a Ba&tors t"at a@ ead to errors
in t"e re!orted resuts.
'- des&rie iits oB a&&ura&@ oB t"e re!orted resut in ters
oB
a. re!orted aues
. de&ia !a&es
&. !ro&edure iitations
d. instruent iitations
e. &ini&a sini&an&e
B. assa@ rane
. &ini&a rane
". e*tent oB inearit@
i. reaents
1. &airators
4. te!erature
. "uidit@
. iration
n. air &urrent
o. interBeren&e
!. &ross-rea&tiit@
. sensitiit@ iits
r. s!e&i&it@
2 - >
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s. reaent an4
,- des&rie "o to s!ot errors in t"e a.
>- des&rie "o to eiinate or iniie t"e sie and
Breuen&@ oB errors.
/- outine a@s to i!roe a&&ura&@7 !re&ision oB resuts.
20- outine !ossie ste!s to i!roe t"e sensitiit@ and
s!e&i&it@ oB et"ods in t"e a.
2- re&onie t"e need Bor &ontinuous deeo!ent and
odi&ations oB e*istin s@stes in t"e a and outside t"e
a.
22- des&rie t"e a1or !re&autions t"at ust e osered in
t"e a to ensure t"e reiaiit@ oB t"e re!orted resuts.
2) CR*",13RTE #ET*"LIS#
-Si. lectures
"b&ecti'es
U!on &o!etion oB t"is &"a!ter7 t"e student i e ae to#
1- IdentiB@ t"e !riar@ iooi&a Bun&tion oB &aro"@drate in
"uans.
2- Des&rie7 in enera ters7 t"e etaois oB
&aro"@drates in "uans.
3- Co!are and &ontrast t"e Bour t@!es oB utiiation oB
u&ose# @&oenesis7 u&oneoenesis7 @&o@sis7
@&oeno@sis.
2 - /
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4- $utine t"e Boration oB ot"er &onstituents7 su&" as
@&oen7 aa&tose7 Bru&tose7 !@rui& a&id7 a&ti& a&id7 Bro
u&ose.
5- Dis&uss t"e i!ortan&e oB t"e anaeroi& and aeroi&
!at"a@s and t"e !entose !"os!"ate s"unt in t"e
etaois oB u&ose.
6- IdentiB@ and e*!ain t"e roe oB t"e Booin "orones in
u&ose etaois
a. Insuin
.
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a. ood u&ose
.
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>- For ea&" test isted eo des&rie t"e rationae7
&ini&a useBuness7 s!e&ien reuireents7 !atient
!re!aration7 !ro&edure7 !rin&i!e(s)7 easureent
used to
&a&uate a&tiit@7 iitations7 and reBeren&e rane(s)#
a -
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4) #I5" CI3"PT,IES
-Three lectures
"b&ecti'es:
Students are ien t"e Bundaenta &on&e!ts oB etaoi&
disorders and t"eir !at"a@s. A&&uuation and suseuent
oer6o oB !re&ursors due to t"e de&ien&@ or ina&tiit@ oB a &ertain
etaoite (aino-a&id &ontainin !rotein or en@e) are
&onsidered t"e asis oB t"ese disorders. Dete&tion oB t"ese
etaoites in ood and t"eir oer6o into t"e urine is &ini&a@
sini&ant.
Dis&uss t"e etaoi& disorders are rare "oeer soe oB t"e are
ore &oon t"an ot"ers and t"ese in&ude#
• :"en@4etonuria# In"erited autosoa re&essie disorder "ere
ear@ dete&tion oB a&&uuatin etaoite is &ru&ia Bor etter
!ronosis
• A4a!tonuria
• T@rosinuria# T@!eI and T@!e II
• Ma!e S@ru! Urine Disease
• Hoo&@stineuria
•C@stinuria
2 - +
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T"e to!i&s &oered in&ude arious et"ods oB dete&tion7 s&reenin
tests and reBeren&e et"ods. Aso &riteria Bor ood and urine
sa!e &oe&tion is dis&ussed
6) PR"TEI5S
-Three lectures
"b&ecti'es:
T"e students are reuired to 4no t"e &ini&a sini&an&e oB
!roteins and to dierentiate eteen arious Bra&tions oB !roteins
de!endin u!on t"eir Bun&tions. As aorator@ te&"nooists t"e
students ust 4no dierent et"ods oB dete&tion and t"eir
under@in !rin&i!es.
T"e to!i&s &oered in&ude#
• Stru&tures oB !roteins7 a!"oteri& !ro!ert@7 &on&e!ts oB
&ataois and anaois
•
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c. A!"a- ouins
d. A!"a-2 ouins
e. eta ouins
f.
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Students are ade aare oB t"e i!ortan&e oB itains as essentia
&o!onent oB dai@ nutrition. I!ortant itains are &oered t"at
in&udes# itain-A7 C7 D7 and &o!e*. T"eir triia naes7
reBeren&e ranes7 dietar@ sour&e and Bun&tions are &oered.
To!i&s in&udin &ini&a s@!tos due to t"eir de&ien&@ or
oerdose are aso e*!ained Booed @ arious et"ods oB
dete&tion "i&" in&ude#
• In-io and in-itro ioassa@s
• Mi&roiooi&a assa@s
• itain oadin tests
• Fouroetri& and Coorietri& ana@sis
• H:5C
• RIA K E5ISA
• A!eroetr@
• Er@t"ro&@te Braiit@ test
• :rot"roin and !artia t"roin test
• A&tiit@ &oe%&ient test
To understand t"e adantaes and disadantaes oB ea&" oB t"e
aoe et"od is des&ries and t"e students are ade e aare oB
t"e reBeren&e et"ods and s&reenin et"ods used.
9) TRCE ELE#E5TS
-Three lectures
"b&ecti'es:
2 - '
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To understand "at t"ese tra&e eeents areQ And to a!!re&iate
t"eir !resen&e in t"e "uan od@.
To understand t"e nutritiona aue and Bun&tiona i!ortan&e oB
essentia7 oderate@ essentia and non-essentia tra&e eeents.
To understand t"e nutritiona aue and Bun&tiona i!ortan&e oB
utra-tra&eeents
Tra&e eeents &oered in t"is &"a!ter in&ude#
• Iron# nora ranes7 !roteins &arriers7 &ini&a sini&an&e7
et"ods oB dete&tion7 transBerrin and Berritin7 Iron saturation
and indin &a!a&it@. Iron !i&ture in arious t@!es oB aneias
• Co!!er# &ini&a sini&an&e7 Bun&tions and trans!ortin
en@e (&eruo!asin)7 et"od oB dete&tion7 t@!es oB sa!e
and interBerin sustan&es
• in Bun&tion7 &ini&a sini&an&e7 et"od oB dete&tion7 t@!es
oB sa!e and interBerin sustan&es
Utra-tra&e eeents &oered in t"is &"a!ter in&ude#
• Seeniu# Fun&tion in asso&iation it" itain-E and
utat"ione !ero*idase7 &ini&a sini&an&e7 et"ods oB
dete&tion7 t@!es oB sa!e and nora rane
•
Mananese# Bun&tion and &ini&a de&ien&@7 t@!e oB sa!ereuired
• Coat# asor!tion in reation to itain 2 and intrinsi&
Ba&tor.
) E5;1#"L"
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"b&ecti'es:
U!on &o!etion oB t"is &"a!ter7 t"e student i e ae to#
- E*!ain t"e dierent Ba&tors ae&tin seru en@e#
• Rate oB entr@ oB en@es into ood
• En@e in"iitors
• Cearan&e oB en@e
2- E*!ain s!e&i&it@ oB seru en@e easureents#
• Test resuts and &ini&a Beatures
• Test !attern re&onition
• Isoen@es
+-
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. - Dis&uss t"e dierent et"ods Bor t"e deterination in seru
and urine.
/- Assess s!e&ien interit@ Bor en@e ana@sis ased on
sa!es
a. &oe&tion
2 - /
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. !re&essin7 and
&. storae &onditions
=) ELECTR"L1TES -5a> ?> Cl> + Ca> #g> Pi
- 5ine lectures
"b&ecti'es:
T"e student ust e ae to
- des&rie t"e !"@siooi&a roes oB Na7 7 C7 Ca7 M K :i
2- des&rie t"e a1or Ba&tors t"at in6uen&e t"e &on&entration oB
t"ese sustan&e in t"e od@ and ood.
+- des&rie t"e &ini&a sini&an&e and &orreations in t"e
easureents oB Na7 7 C7 Ca7 M K :i.
- des&rie t"e s!e&ien reuireents Bor t"e deterination oB
Na7 7 C7 Ca7 M K :i.
- des&rie t"e !ossie sour&es oB errors in t"e deterination oB
Na7 7 C7 Ca7 M K :i.
'- des&rie t"e ee&ts oB su&" errors on t"e resuts otained Bor
ea&" oB t"e ana@tes (i.e Base in&rease or Base de&rease).
,- des&rie a1or interBerin Ba&tors t"at a@ ae&t t"e resuts.
>- des&rie "o to eiinate interBeren&es.
2 - 20
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/- des&rie t"e a@s to iniie or eiinate t"e arious sour&es
oB errors.
0-des&rie t"e reBeren&e et"ods Bor deterination oB Na7 7 C7
Ca7
M K :i.
-des&rie t"e ost &oon@ used et"ods Bor t"e
deterination oB
Na7 7 C7 Ca7 M K :i "i&" are used in t"e &ini&a a.
2-e*!ain "o to use a resuts to dierentiate eteen
arious t@!es
oB "@!onatreia.
+-e*!ain t"e ter !ani& aues.
-ist t"e !ani& aues Bor Na K .
-e*!ain t"e reations"i! eteen a&id ase disturan&es and
Na7 7
C7 Ca K M.
'-rite t"e Ma&5ean-Hastins Forua Bor t"e &a&uation oB
ionied
Ca (Ca2).
,-&a&uate t"e Ca2 &on&entration usin t"e Ma&5ean-Hastins
Forua.
>-ist t"e &onditions Bor t"e use oB t"e Ma&5ean-Hastins
Forua
Bor t"e &a&uation oB Ca2.
/-des&rie t"e &oon et"ods used to easure Ca2 .
20-rite t"e Borua used to &a&uate t"e anion a! (A
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2+-des&rie t"e &ini&a sini&an&e Bor t"e &a&uation oB A< in
ood
a. in&reased A<
. de&reased A<
&. a!!arent@ nora A<
2-des&rie t"e &ini&a sini&an&e Bor t"e &a&uation oB A< in
urine.
2-e*!ain t"e ee&t oB &oined errors on t"e &onsiderations
oB t"e
A< aues in &ini&a situations oB etaoi& a&idosis.
2'-e*!ain "o A< is used in t"e &ini&a a.
LER5I5< RES"URCES
,%, +OO) & R%#I*G $#,%RI#L)#
Assigned Textbook :
Cinica C!e"ist#$% inci'es% oced(#es% and Co##eations% 3#d )d.
*$ +ic!ae *is!o'% )d,a#d odd$% and anet /(ben
.*. 0i''incott % &!iade'!ia% 25o# atest edition.
eco""ended efe#ences :
1- Cinica /iagnosis and +anage"ent% b$ o!n *e#na#d en#$%ed.
2 - 22
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.*. a(dne#s% &!iade'!ia.atest edition.
2- (nda"entas of Cinica C!e"ist#$ Tiet% atest edition.% .*. a(nde#s a'an
&esce. atest edition.
3- Cinica C!e"ist# T!eo#$ Ana$sis Co##eation% atest ed. C.. +osb$
Cinica C!e"ist#$ in /iagnosis and T#eat"ent% ;ia &anne%
atest edition.
/%+)I,% #R%))%)
!tt':
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Fa&ut@ oB a!!ied edi&a s&ien&es7Feae se&tion
2 - 2
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L#+OR#,OR "O'R)
PRCTICLST!o/Week
#LE
Sat 02#00 9 0#00 (
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:RACTICA5 5A$RAT$R= $JECTIES
Hain attended a oB t"e !ra&ti&a a sessions7 istened to and
understood t"e e*!anation oB t"e !ro&edure done7 at&"ed a
deonstration @ t"e instru&tor (or t"e a te&"nooist in &"are)7
and read a t"e aterias assined7 t"e student ust e ae to
!erBor a oB t"e e*!erients (tests7 !ro&edures7 et&.) e*!ained
and tau"t. T"e student ust e ae to e*"iit a satisBa&tor@
&o!eten&e ee @ a&"iein no ess t"an '0G oB an@ ien
!ra&ti&a e*a !erBored it"in a suita@ assined tie.
Genera6 Laborator7 Objectives
. Des&rie t"e t@!es7 !re!aration7 use and storae oB &"ei&as
used in
a &"eistr@ aorator@.
2. Dis&uss t"e &o!osition7 use7 and iitations oB aorator@
assare7 !asti& are and tuin.
+. IdentiB@ t"e arious t@!es oB ouetri& eui!ent in
&oon usae in t"e &"eistr@ aorator@.
. Des&rie !roto&os Bor t"e &airation oB ouetri&
eui!ent7 its use and aintenan&e.
. E*!ain t"e t"eories oB o!eration oB &entriBues and
aan&es.
'. Dis&uss &airation aintenan&e reuireents7 and
enironenta Ba&tors inoed in t"e use oB &entriBues
and aan&es.
,. Des&rie t"e !rin&i!es oB !ro&edures used to !re!are sa!es
and soutions Bor ana@sis.
>. Dis&uss t"e &o!onents7 !re!aration7 sour&es oB error and
e*!ressions oB &on&entration oB soutions.
2 - 2'
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/. Dene t"e &o!onents oB uer s@stes and its roe in t"e
aintenan&e oB !H.
0. Des&rie t"e t@!es oB saBet@ "aards Bound in t"e
&"eistr@ aorator@.
. IdentiB@ s!e&i& !re&autions and saBet@ eui!ent
used to iniie or !reent aorator@ "aards.
5aorator@ To!i&s#
. Cin. C"e. 5a Fun&tion
2. Sour&es oB Errors E*er&ise
+. 5a Mat" E*er&ise
.
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+. ;"en ien asor!tiit@ &onstant and &on&entration7 &a&uate
t"e asoran&e.
. Assess auin usin t"e C< d@e et"od it" resuts it"in G
oB easured aue.
. Co!are and &ontrast one-!oint &airation it" t"at oB a
standard &ure.
'. :erBor ee&tro!"oresis and identiB@ a nora !attern.
,. ;"en ien a ist oB asoran&e aues &a&uate t"e &"ane in
asoran&e.
>. ;"en ien asoran&e aues7 ent" oB i"t !at" and oar
asor!tiit@ oB a sustan&e7 &a&uate t"e U aue.
/. Co!are and &ontrast end!oint and 4ineti& en@e assa@s.
0. Ad1ust t"e as!irati'n rate oB t"e atoier on t"e 6ae
!"otoeter.
. Set t"e standards oB t"e 6ae !"otoeter.
2. Ana@e &ontros and !atient sa!es on t"e 6ae !"otoeter
+. :erBor t"e &"oride deterination and &a&uate t"e
&on&entration oB
t"e &ontro and !atient sa!es usin t"e &"oride eter.
. :erBor it"in G and it" a&&e!tae te&"niue t"e arious
u&ose
test et"ods.
. :erBor t"e Booin assa@s it" a&&e!tae te&"niue7
!re&ision7
and a&&ura&@#
'. A&&ession s!e&iens and eauate t"e as to t"eir suitaiit@ Bor
t"e deterination reuested.
,. :re!are aiuots oB s!e&iens and distriute t"e Bor ana@sis.
>. Maintain re&ord 4ee!in Bor s!e&iens.
/. :re!are reaents a&&ordin to !res&ried dire&tions.
2 - 2>
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20. Deonstrate e%&ient o!eration oB aorator@ instruents to
a&&e!tae ees Bor t"e instru&tor.
2. :erBor and inter!ret ana@ses on !atient s!e&iens and
&ontros
a&&ordin to !roto&o.
22. Eauate test resuts it" resuts a&&e!tae to t"e instru&tor.
Course Re@uirements +
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to as7 t"e instru&tor a@7 ut is not oiated to e ait"draa.
2 - +0
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%#L'#,IO* ),R#,%GI%)5GR#I*G
Note# IB a student ust e asent on t"e da@ oB a test7 "e8s"eust notiB@ t"e instru&tor !rior to test tie in order to eaoed to ta4e a a4e-u! test. A rade oB ero (0) i eassined iB t"e instru&tor is not notied. IB t"e student e*&eeds
t"e a*iu asen&es oB 0G7 t"is i resut in "is8"er eindro!!ed Bro t"e &ourse and Bro t"e M5T !rora.
GR#I*G )C#L%
T"e Booin rade s&ae a!!ies t"rou"out t"e &ourse#
E*&eent O /0.0G - 00.0G
er@ ood O >0.0G - >/.0G