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DOWNSTREAM
(cell)
Introduction of
Boprocess Technology
Nur Istianah
22112018 Nur Istianah-PTB Downstream process-
2016
Flotation
bull Flotation is another method to remove solid (cells) from fermentation broth using air bubbles to float protein
bull We may use different kinds of high shear-force devices to make homogeneous solutions for liberating intracellular enzymes
bull Non-mechanical methods are also used to break down the cell wall and to release intracellular enzymes or proteins
Aeration
bull Particulates can be removed from aqueous suspension by attachment to rising air bubbles
bull This method is known as flotation which is widely used for recovery of small particlesfrom aqueous suspension minerals
bull A related technique for flotation uses airndashwater surface tension to strip out proteins from the broth solution and accumulate them in a high protein
Cell recovery
bull Click to add text
Aeration
bull Flotation is applied to concentrate Acinetobacter cerificans for production of SCP Acinetobacter cerificans is a suitable microorganism used in the production of SCP
bull The synthesised protein is concentrated by aeration using the flotation method
Cell recovery
bull Downstream processing of fermentation broths usually begins with separation of cells by filtration or centrifugation
bull A combination of bioprocesses is required for product recovery Separation of products such as ethanol citric acid and antibiotics is extracellular but isolation of enzymes inside the cells requires cell rupture
bull Cells are broken down by lysis of the cell wall Cell disruption is used for downstream product recovery Removal of biomass from the extracted product is necessary
Cell disruption
bull Disrupsi sel adalah proses rusaknya dinding sel yang menyebabkan keluarnya senyawa biokimia dari dalamnya Perusakan dinding sel ini biasanya disertai dengan lisis atau robeknya dinding sel
Methods
bull Mekanis bull Metode ini disertari dengan penggunaan energy
mekanik seperti penggerusan biomassa disertai abrasi pengadukan dengan kecepatan tinggi pemompaan dengan tekanan tinggi dan ultrasonik
bull Non-mekanis bull Metode ini tidak disertari dengan penggunaan energy
mekanik melaikan dengan bantuan bahan kimia ataupun energy non-mekanik Beberapa metode non-mekanik yang biasa digunakan yaitu terapi osmotik pembekuan thawing perusakan enzimatis serta menggunakan bantuan pelarut atau deterjen
Role of cell disruption
bull Disrupsi sangat sering digunakan untuk recovery produk dari biomassa termasuk isolasi enzim dari sel secara intraseluler Adapun biomassa yang keluar dari ekstraksi atau recovery kemudian dipisahkan dan dikeluarkan sebagai produk samping
Mechanism
bull Lysis of cells for intracellular product recovery
bull Extraction of lysed or ground cells
bull Removal of unconverted soluble substrate
bull Removal of biomass from extracted product
Mechanism
bull The choice of filtration (batch continuous vacuum cross flow etc)
bull Centrifuge (vertical rotor horizontal rotor)
bull Sedimentation (andor coagulation)
Methods of cell lysis
bull Osmotic shock bull Freezing
bull Solvent bull Detergents
bull homogeniser bull organic solvent extraction
22112018 Nur Istianah-PTB Downstream process-
2016
CASE STUDY PROCESS INTEGRATION OF CELL DISRUPTION AND FLUIDISED BED ADSORPTION FOR THE RECOVERY OF LABILE INTRACELLULAR ENZYMES
22112018 Nur Istianah-PTB Downstream process-
2016
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
22112018 Nur Istianah-PTB Downstream process-
2016
Flotation
bull Flotation is another method to remove solid (cells) from fermentation broth using air bubbles to float protein
bull We may use different kinds of high shear-force devices to make homogeneous solutions for liberating intracellular enzymes
bull Non-mechanical methods are also used to break down the cell wall and to release intracellular enzymes or proteins
Aeration
bull Particulates can be removed from aqueous suspension by attachment to rising air bubbles
bull This method is known as flotation which is widely used for recovery of small particlesfrom aqueous suspension minerals
bull A related technique for flotation uses airndashwater surface tension to strip out proteins from the broth solution and accumulate them in a high protein
Cell recovery
bull Click to add text
Aeration
bull Flotation is applied to concentrate Acinetobacter cerificans for production of SCP Acinetobacter cerificans is a suitable microorganism used in the production of SCP
bull The synthesised protein is concentrated by aeration using the flotation method
Cell recovery
bull Downstream processing of fermentation broths usually begins with separation of cells by filtration or centrifugation
bull A combination of bioprocesses is required for product recovery Separation of products such as ethanol citric acid and antibiotics is extracellular but isolation of enzymes inside the cells requires cell rupture
bull Cells are broken down by lysis of the cell wall Cell disruption is used for downstream product recovery Removal of biomass from the extracted product is necessary
Cell disruption
bull Disrupsi sel adalah proses rusaknya dinding sel yang menyebabkan keluarnya senyawa biokimia dari dalamnya Perusakan dinding sel ini biasanya disertai dengan lisis atau robeknya dinding sel
Methods
bull Mekanis bull Metode ini disertari dengan penggunaan energy
mekanik seperti penggerusan biomassa disertai abrasi pengadukan dengan kecepatan tinggi pemompaan dengan tekanan tinggi dan ultrasonik
bull Non-mekanis bull Metode ini tidak disertari dengan penggunaan energy
mekanik melaikan dengan bantuan bahan kimia ataupun energy non-mekanik Beberapa metode non-mekanik yang biasa digunakan yaitu terapi osmotik pembekuan thawing perusakan enzimatis serta menggunakan bantuan pelarut atau deterjen
Role of cell disruption
bull Disrupsi sangat sering digunakan untuk recovery produk dari biomassa termasuk isolasi enzim dari sel secara intraseluler Adapun biomassa yang keluar dari ekstraksi atau recovery kemudian dipisahkan dan dikeluarkan sebagai produk samping
Mechanism
bull Lysis of cells for intracellular product recovery
bull Extraction of lysed or ground cells
bull Removal of unconverted soluble substrate
bull Removal of biomass from extracted product
Mechanism
bull The choice of filtration (batch continuous vacuum cross flow etc)
bull Centrifuge (vertical rotor horizontal rotor)
bull Sedimentation (andor coagulation)
Methods of cell lysis
bull Osmotic shock bull Freezing
bull Solvent bull Detergents
bull homogeniser bull organic solvent extraction
22112018 Nur Istianah-PTB Downstream process-
2016
CASE STUDY PROCESS INTEGRATION OF CELL DISRUPTION AND FLUIDISED BED ADSORPTION FOR THE RECOVERY OF LABILE INTRACELLULAR ENZYMES
22112018 Nur Istianah-PTB Downstream process-
2016
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Flotation
bull Flotation is another method to remove solid (cells) from fermentation broth using air bubbles to float protein
bull We may use different kinds of high shear-force devices to make homogeneous solutions for liberating intracellular enzymes
bull Non-mechanical methods are also used to break down the cell wall and to release intracellular enzymes or proteins
Aeration
bull Particulates can be removed from aqueous suspension by attachment to rising air bubbles
bull This method is known as flotation which is widely used for recovery of small particlesfrom aqueous suspension minerals
bull A related technique for flotation uses airndashwater surface tension to strip out proteins from the broth solution and accumulate them in a high protein
Cell recovery
bull Click to add text
Aeration
bull Flotation is applied to concentrate Acinetobacter cerificans for production of SCP Acinetobacter cerificans is a suitable microorganism used in the production of SCP
bull The synthesised protein is concentrated by aeration using the flotation method
Cell recovery
bull Downstream processing of fermentation broths usually begins with separation of cells by filtration or centrifugation
bull A combination of bioprocesses is required for product recovery Separation of products such as ethanol citric acid and antibiotics is extracellular but isolation of enzymes inside the cells requires cell rupture
bull Cells are broken down by lysis of the cell wall Cell disruption is used for downstream product recovery Removal of biomass from the extracted product is necessary
Cell disruption
bull Disrupsi sel adalah proses rusaknya dinding sel yang menyebabkan keluarnya senyawa biokimia dari dalamnya Perusakan dinding sel ini biasanya disertai dengan lisis atau robeknya dinding sel
Methods
bull Mekanis bull Metode ini disertari dengan penggunaan energy
mekanik seperti penggerusan biomassa disertai abrasi pengadukan dengan kecepatan tinggi pemompaan dengan tekanan tinggi dan ultrasonik
bull Non-mekanis bull Metode ini tidak disertari dengan penggunaan energy
mekanik melaikan dengan bantuan bahan kimia ataupun energy non-mekanik Beberapa metode non-mekanik yang biasa digunakan yaitu terapi osmotik pembekuan thawing perusakan enzimatis serta menggunakan bantuan pelarut atau deterjen
Role of cell disruption
bull Disrupsi sangat sering digunakan untuk recovery produk dari biomassa termasuk isolasi enzim dari sel secara intraseluler Adapun biomassa yang keluar dari ekstraksi atau recovery kemudian dipisahkan dan dikeluarkan sebagai produk samping
Mechanism
bull Lysis of cells for intracellular product recovery
bull Extraction of lysed or ground cells
bull Removal of unconverted soluble substrate
bull Removal of biomass from extracted product
Mechanism
bull The choice of filtration (batch continuous vacuum cross flow etc)
bull Centrifuge (vertical rotor horizontal rotor)
bull Sedimentation (andor coagulation)
Methods of cell lysis
bull Osmotic shock bull Freezing
bull Solvent bull Detergents
bull homogeniser bull organic solvent extraction
22112018 Nur Istianah-PTB Downstream process-
2016
CASE STUDY PROCESS INTEGRATION OF CELL DISRUPTION AND FLUIDISED BED ADSORPTION FOR THE RECOVERY OF LABILE INTRACELLULAR ENZYMES
22112018 Nur Istianah-PTB Downstream process-
2016
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Aeration
bull Particulates can be removed from aqueous suspension by attachment to rising air bubbles
bull This method is known as flotation which is widely used for recovery of small particlesfrom aqueous suspension minerals
bull A related technique for flotation uses airndashwater surface tension to strip out proteins from the broth solution and accumulate them in a high protein
Cell recovery
bull Click to add text
Aeration
bull Flotation is applied to concentrate Acinetobacter cerificans for production of SCP Acinetobacter cerificans is a suitable microorganism used in the production of SCP
bull The synthesised protein is concentrated by aeration using the flotation method
Cell recovery
bull Downstream processing of fermentation broths usually begins with separation of cells by filtration or centrifugation
bull A combination of bioprocesses is required for product recovery Separation of products such as ethanol citric acid and antibiotics is extracellular but isolation of enzymes inside the cells requires cell rupture
bull Cells are broken down by lysis of the cell wall Cell disruption is used for downstream product recovery Removal of biomass from the extracted product is necessary
Cell disruption
bull Disrupsi sel adalah proses rusaknya dinding sel yang menyebabkan keluarnya senyawa biokimia dari dalamnya Perusakan dinding sel ini biasanya disertai dengan lisis atau robeknya dinding sel
Methods
bull Mekanis bull Metode ini disertari dengan penggunaan energy
mekanik seperti penggerusan biomassa disertai abrasi pengadukan dengan kecepatan tinggi pemompaan dengan tekanan tinggi dan ultrasonik
bull Non-mekanis bull Metode ini tidak disertari dengan penggunaan energy
mekanik melaikan dengan bantuan bahan kimia ataupun energy non-mekanik Beberapa metode non-mekanik yang biasa digunakan yaitu terapi osmotik pembekuan thawing perusakan enzimatis serta menggunakan bantuan pelarut atau deterjen
Role of cell disruption
bull Disrupsi sangat sering digunakan untuk recovery produk dari biomassa termasuk isolasi enzim dari sel secara intraseluler Adapun biomassa yang keluar dari ekstraksi atau recovery kemudian dipisahkan dan dikeluarkan sebagai produk samping
Mechanism
bull Lysis of cells for intracellular product recovery
bull Extraction of lysed or ground cells
bull Removal of unconverted soluble substrate
bull Removal of biomass from extracted product
Mechanism
bull The choice of filtration (batch continuous vacuum cross flow etc)
bull Centrifuge (vertical rotor horizontal rotor)
bull Sedimentation (andor coagulation)
Methods of cell lysis
bull Osmotic shock bull Freezing
bull Solvent bull Detergents
bull homogeniser bull organic solvent extraction
22112018 Nur Istianah-PTB Downstream process-
2016
CASE STUDY PROCESS INTEGRATION OF CELL DISRUPTION AND FLUIDISED BED ADSORPTION FOR THE RECOVERY OF LABILE INTRACELLULAR ENZYMES
22112018 Nur Istianah-PTB Downstream process-
2016
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Cell recovery
bull Click to add text
Aeration
bull Flotation is applied to concentrate Acinetobacter cerificans for production of SCP Acinetobacter cerificans is a suitable microorganism used in the production of SCP
bull The synthesised protein is concentrated by aeration using the flotation method
Cell recovery
bull Downstream processing of fermentation broths usually begins with separation of cells by filtration or centrifugation
bull A combination of bioprocesses is required for product recovery Separation of products such as ethanol citric acid and antibiotics is extracellular but isolation of enzymes inside the cells requires cell rupture
bull Cells are broken down by lysis of the cell wall Cell disruption is used for downstream product recovery Removal of biomass from the extracted product is necessary
Cell disruption
bull Disrupsi sel adalah proses rusaknya dinding sel yang menyebabkan keluarnya senyawa biokimia dari dalamnya Perusakan dinding sel ini biasanya disertai dengan lisis atau robeknya dinding sel
Methods
bull Mekanis bull Metode ini disertari dengan penggunaan energy
mekanik seperti penggerusan biomassa disertai abrasi pengadukan dengan kecepatan tinggi pemompaan dengan tekanan tinggi dan ultrasonik
bull Non-mekanis bull Metode ini tidak disertari dengan penggunaan energy
mekanik melaikan dengan bantuan bahan kimia ataupun energy non-mekanik Beberapa metode non-mekanik yang biasa digunakan yaitu terapi osmotik pembekuan thawing perusakan enzimatis serta menggunakan bantuan pelarut atau deterjen
Role of cell disruption
bull Disrupsi sangat sering digunakan untuk recovery produk dari biomassa termasuk isolasi enzim dari sel secara intraseluler Adapun biomassa yang keluar dari ekstraksi atau recovery kemudian dipisahkan dan dikeluarkan sebagai produk samping
Mechanism
bull Lysis of cells for intracellular product recovery
bull Extraction of lysed or ground cells
bull Removal of unconverted soluble substrate
bull Removal of biomass from extracted product
Mechanism
bull The choice of filtration (batch continuous vacuum cross flow etc)
bull Centrifuge (vertical rotor horizontal rotor)
bull Sedimentation (andor coagulation)
Methods of cell lysis
bull Osmotic shock bull Freezing
bull Solvent bull Detergents
bull homogeniser bull organic solvent extraction
22112018 Nur Istianah-PTB Downstream process-
2016
CASE STUDY PROCESS INTEGRATION OF CELL DISRUPTION AND FLUIDISED BED ADSORPTION FOR THE RECOVERY OF LABILE INTRACELLULAR ENZYMES
22112018 Nur Istianah-PTB Downstream process-
2016
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Aeration
bull Flotation is applied to concentrate Acinetobacter cerificans for production of SCP Acinetobacter cerificans is a suitable microorganism used in the production of SCP
bull The synthesised protein is concentrated by aeration using the flotation method
Cell recovery
bull Downstream processing of fermentation broths usually begins with separation of cells by filtration or centrifugation
bull A combination of bioprocesses is required for product recovery Separation of products such as ethanol citric acid and antibiotics is extracellular but isolation of enzymes inside the cells requires cell rupture
bull Cells are broken down by lysis of the cell wall Cell disruption is used for downstream product recovery Removal of biomass from the extracted product is necessary
Cell disruption
bull Disrupsi sel adalah proses rusaknya dinding sel yang menyebabkan keluarnya senyawa biokimia dari dalamnya Perusakan dinding sel ini biasanya disertai dengan lisis atau robeknya dinding sel
Methods
bull Mekanis bull Metode ini disertari dengan penggunaan energy
mekanik seperti penggerusan biomassa disertai abrasi pengadukan dengan kecepatan tinggi pemompaan dengan tekanan tinggi dan ultrasonik
bull Non-mekanis bull Metode ini tidak disertari dengan penggunaan energy
mekanik melaikan dengan bantuan bahan kimia ataupun energy non-mekanik Beberapa metode non-mekanik yang biasa digunakan yaitu terapi osmotik pembekuan thawing perusakan enzimatis serta menggunakan bantuan pelarut atau deterjen
Role of cell disruption
bull Disrupsi sangat sering digunakan untuk recovery produk dari biomassa termasuk isolasi enzim dari sel secara intraseluler Adapun biomassa yang keluar dari ekstraksi atau recovery kemudian dipisahkan dan dikeluarkan sebagai produk samping
Mechanism
bull Lysis of cells for intracellular product recovery
bull Extraction of lysed or ground cells
bull Removal of unconverted soluble substrate
bull Removal of biomass from extracted product
Mechanism
bull The choice of filtration (batch continuous vacuum cross flow etc)
bull Centrifuge (vertical rotor horizontal rotor)
bull Sedimentation (andor coagulation)
Methods of cell lysis
bull Osmotic shock bull Freezing
bull Solvent bull Detergents
bull homogeniser bull organic solvent extraction
22112018 Nur Istianah-PTB Downstream process-
2016
CASE STUDY PROCESS INTEGRATION OF CELL DISRUPTION AND FLUIDISED BED ADSORPTION FOR THE RECOVERY OF LABILE INTRACELLULAR ENZYMES
22112018 Nur Istianah-PTB Downstream process-
2016
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Cell recovery
bull Downstream processing of fermentation broths usually begins with separation of cells by filtration or centrifugation
bull A combination of bioprocesses is required for product recovery Separation of products such as ethanol citric acid and antibiotics is extracellular but isolation of enzymes inside the cells requires cell rupture
bull Cells are broken down by lysis of the cell wall Cell disruption is used for downstream product recovery Removal of biomass from the extracted product is necessary
Cell disruption
bull Disrupsi sel adalah proses rusaknya dinding sel yang menyebabkan keluarnya senyawa biokimia dari dalamnya Perusakan dinding sel ini biasanya disertai dengan lisis atau robeknya dinding sel
Methods
bull Mekanis bull Metode ini disertari dengan penggunaan energy
mekanik seperti penggerusan biomassa disertai abrasi pengadukan dengan kecepatan tinggi pemompaan dengan tekanan tinggi dan ultrasonik
bull Non-mekanis bull Metode ini tidak disertari dengan penggunaan energy
mekanik melaikan dengan bantuan bahan kimia ataupun energy non-mekanik Beberapa metode non-mekanik yang biasa digunakan yaitu terapi osmotik pembekuan thawing perusakan enzimatis serta menggunakan bantuan pelarut atau deterjen
Role of cell disruption
bull Disrupsi sangat sering digunakan untuk recovery produk dari biomassa termasuk isolasi enzim dari sel secara intraseluler Adapun biomassa yang keluar dari ekstraksi atau recovery kemudian dipisahkan dan dikeluarkan sebagai produk samping
Mechanism
bull Lysis of cells for intracellular product recovery
bull Extraction of lysed or ground cells
bull Removal of unconverted soluble substrate
bull Removal of biomass from extracted product
Mechanism
bull The choice of filtration (batch continuous vacuum cross flow etc)
bull Centrifuge (vertical rotor horizontal rotor)
bull Sedimentation (andor coagulation)
Methods of cell lysis
bull Osmotic shock bull Freezing
bull Solvent bull Detergents
bull homogeniser bull organic solvent extraction
22112018 Nur Istianah-PTB Downstream process-
2016
CASE STUDY PROCESS INTEGRATION OF CELL DISRUPTION AND FLUIDISED BED ADSORPTION FOR THE RECOVERY OF LABILE INTRACELLULAR ENZYMES
22112018 Nur Istianah-PTB Downstream process-
2016
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Cell disruption
bull Disrupsi sel adalah proses rusaknya dinding sel yang menyebabkan keluarnya senyawa biokimia dari dalamnya Perusakan dinding sel ini biasanya disertai dengan lisis atau robeknya dinding sel
Methods
bull Mekanis bull Metode ini disertari dengan penggunaan energy
mekanik seperti penggerusan biomassa disertai abrasi pengadukan dengan kecepatan tinggi pemompaan dengan tekanan tinggi dan ultrasonik
bull Non-mekanis bull Metode ini tidak disertari dengan penggunaan energy
mekanik melaikan dengan bantuan bahan kimia ataupun energy non-mekanik Beberapa metode non-mekanik yang biasa digunakan yaitu terapi osmotik pembekuan thawing perusakan enzimatis serta menggunakan bantuan pelarut atau deterjen
Role of cell disruption
bull Disrupsi sangat sering digunakan untuk recovery produk dari biomassa termasuk isolasi enzim dari sel secara intraseluler Adapun biomassa yang keluar dari ekstraksi atau recovery kemudian dipisahkan dan dikeluarkan sebagai produk samping
Mechanism
bull Lysis of cells for intracellular product recovery
bull Extraction of lysed or ground cells
bull Removal of unconverted soluble substrate
bull Removal of biomass from extracted product
Mechanism
bull The choice of filtration (batch continuous vacuum cross flow etc)
bull Centrifuge (vertical rotor horizontal rotor)
bull Sedimentation (andor coagulation)
Methods of cell lysis
bull Osmotic shock bull Freezing
bull Solvent bull Detergents
bull homogeniser bull organic solvent extraction
22112018 Nur Istianah-PTB Downstream process-
2016
CASE STUDY PROCESS INTEGRATION OF CELL DISRUPTION AND FLUIDISED BED ADSORPTION FOR THE RECOVERY OF LABILE INTRACELLULAR ENZYMES
22112018 Nur Istianah-PTB Downstream process-
2016
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Methods
bull Mekanis bull Metode ini disertari dengan penggunaan energy
mekanik seperti penggerusan biomassa disertai abrasi pengadukan dengan kecepatan tinggi pemompaan dengan tekanan tinggi dan ultrasonik
bull Non-mekanis bull Metode ini tidak disertari dengan penggunaan energy
mekanik melaikan dengan bantuan bahan kimia ataupun energy non-mekanik Beberapa metode non-mekanik yang biasa digunakan yaitu terapi osmotik pembekuan thawing perusakan enzimatis serta menggunakan bantuan pelarut atau deterjen
Role of cell disruption
bull Disrupsi sangat sering digunakan untuk recovery produk dari biomassa termasuk isolasi enzim dari sel secara intraseluler Adapun biomassa yang keluar dari ekstraksi atau recovery kemudian dipisahkan dan dikeluarkan sebagai produk samping
Mechanism
bull Lysis of cells for intracellular product recovery
bull Extraction of lysed or ground cells
bull Removal of unconverted soluble substrate
bull Removal of biomass from extracted product
Mechanism
bull The choice of filtration (batch continuous vacuum cross flow etc)
bull Centrifuge (vertical rotor horizontal rotor)
bull Sedimentation (andor coagulation)
Methods of cell lysis
bull Osmotic shock bull Freezing
bull Solvent bull Detergents
bull homogeniser bull organic solvent extraction
22112018 Nur Istianah-PTB Downstream process-
2016
CASE STUDY PROCESS INTEGRATION OF CELL DISRUPTION AND FLUIDISED BED ADSORPTION FOR THE RECOVERY OF LABILE INTRACELLULAR ENZYMES
22112018 Nur Istianah-PTB Downstream process-
2016
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Role of cell disruption
bull Disrupsi sangat sering digunakan untuk recovery produk dari biomassa termasuk isolasi enzim dari sel secara intraseluler Adapun biomassa yang keluar dari ekstraksi atau recovery kemudian dipisahkan dan dikeluarkan sebagai produk samping
Mechanism
bull Lysis of cells for intracellular product recovery
bull Extraction of lysed or ground cells
bull Removal of unconverted soluble substrate
bull Removal of biomass from extracted product
Mechanism
bull The choice of filtration (batch continuous vacuum cross flow etc)
bull Centrifuge (vertical rotor horizontal rotor)
bull Sedimentation (andor coagulation)
Methods of cell lysis
bull Osmotic shock bull Freezing
bull Solvent bull Detergents
bull homogeniser bull organic solvent extraction
22112018 Nur Istianah-PTB Downstream process-
2016
CASE STUDY PROCESS INTEGRATION OF CELL DISRUPTION AND FLUIDISED BED ADSORPTION FOR THE RECOVERY OF LABILE INTRACELLULAR ENZYMES
22112018 Nur Istianah-PTB Downstream process-
2016
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Mechanism
bull Lysis of cells for intracellular product recovery
bull Extraction of lysed or ground cells
bull Removal of unconverted soluble substrate
bull Removal of biomass from extracted product
Mechanism
bull The choice of filtration (batch continuous vacuum cross flow etc)
bull Centrifuge (vertical rotor horizontal rotor)
bull Sedimentation (andor coagulation)
Methods of cell lysis
bull Osmotic shock bull Freezing
bull Solvent bull Detergents
bull homogeniser bull organic solvent extraction
22112018 Nur Istianah-PTB Downstream process-
2016
CASE STUDY PROCESS INTEGRATION OF CELL DISRUPTION AND FLUIDISED BED ADSORPTION FOR THE RECOVERY OF LABILE INTRACELLULAR ENZYMES
22112018 Nur Istianah-PTB Downstream process-
2016
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Mechanism
bull The choice of filtration (batch continuous vacuum cross flow etc)
bull Centrifuge (vertical rotor horizontal rotor)
bull Sedimentation (andor coagulation)
Methods of cell lysis
bull Osmotic shock bull Freezing
bull Solvent bull Detergents
bull homogeniser bull organic solvent extraction
22112018 Nur Istianah-PTB Downstream process-
2016
CASE STUDY PROCESS INTEGRATION OF CELL DISRUPTION AND FLUIDISED BED ADSORPTION FOR THE RECOVERY OF LABILE INTRACELLULAR ENZYMES
22112018 Nur Istianah-PTB Downstream process-
2016
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Methods of cell lysis
bull Osmotic shock bull Freezing
bull Solvent bull Detergents
bull homogeniser bull organic solvent extraction
22112018 Nur Istianah-PTB Downstream process-
2016
CASE STUDY PROCESS INTEGRATION OF CELL DISRUPTION AND FLUIDISED BED ADSORPTION FOR THE RECOVERY OF LABILE INTRACELLULAR ENZYMES
22112018 Nur Istianah-PTB Downstream process-
2016
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
22112018 Nur Istianah-PTB Downstream process-
2016
CASE STUDY PROCESS INTEGRATION OF CELL DISRUPTION AND FLUIDISED BED ADSORPTION FOR THE RECOVERY OF LABILE INTRACELLULAR ENZYMES
22112018 Nur Istianah-PTB Downstream process-
2016
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
CASE STUDY PROCESS INTEGRATION OF CELL DISRUPTION AND FLUIDISED BED ADSORPTION FOR THE RECOVERY OF LABILE INTRACELLULAR ENZYMES
22112018 Nur Istianah-PTB Downstream process-
2016
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Case study
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Case study
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Case study
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Cell recovery
bull Application of charges and filtration may separate protein very efficiently
bull Electrokinetic deposition uses voltage gradients of 1050 Vcm to produce solid biomass with densities of up to 40 wv
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
Case study
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
THANKS FOR YOUR ATTENTION
The best person is those who always share goodness
