CHROMATOGRAPHY

Think about foodCare about safety

wwwmn-netcom

wwwmn-netcomMACHEREY-NAGEL GmbH amp Co KG middot Neumann-Neander-Str 6ndash8 middot 52355 Duumlren middot DeutschlandFrankreichMACHEREY-NAGEL EURLTel +33 388 68 22 68Fax +33 388 51 76 88E-Mail sales-frmn-netcom

SchweizMACHEREY-NAGEL AGTel +41 62 388 55 00Fax +41 62 388 55 05E-Mail sales-chmn-netcom

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MACHEREY-NAGEL

EN ISO 9001 2008ZERTIFIZIERT

USAMACHEREY-NAGEL IncTel +1 484 821 0984Fax +1 484 821 1272E-Mail sales-usmn-netcom

Vol 8Additives

infomn-netcom wwwmn-netcom2 3

Focus on additives The global production and trading of food and feed products makes monitoring and control difficult

Only analysis of food and raw materials for the production of food can ensure safe and edible food Food safety is an essential part of our quality of life

MN shows examples for food analysis that will help you with your work and make our food safer

Vol 8 Food additives

Food stuffs are frequently supplemented with additives in order to regulate or stabilize flavor color shelf life nutritional value and texture Food additives also ensure a smooth production process [FDA ndash httpwwwfdagovFoodIngredientsPackagingLabelingFoodAdditivesIngredi-entsucm094211htm]

Within the EU food additives are regulated by the Euro-pean Food Safety Authority (EFSA)

Outside the EU a Joint Expert Committee on Food Ad-ditives (JECFA) has been established by the Food and Agriculture Organization (FAO) and the World Health Or-ganization (WHO) [FAO ndash httpwwwfaoorgfoodfood-safe-ty-qualityscientific-advicejecfaen]

infomn-netcom wwwmn-netcom2 3

Compounds of interestOverview of selected food additives according to FDA

Types of ingredients Purpose function

Examples of uses Examples Typical molecules

Color additivesOffset color loss due to exposure to light air temperature extremes moisture and storage conditionsCorrect natural variations in colorEnhance colors that occur naturallyProvide color to colorless and ldquofunrdquo foods

Many processed foods eg candies snack foods margarine cheese soft drinks jams jellies gelatins pudding and pie fillings

FDampC Blue Nos 1 and 2 FDampC Yellow Nos 5 and 6 Orange B Ponceau 4R Beta-carotene (Note Exempt color additives are not required to be declared by name on labels but may be declared simply as colorings or color added)

N N

SO3ndash Na+

SO3ndash Na+

SO3ndash Na+

HO

Flavor enhancersEnhance flavors already present in foods (without providing their own separate flavor)

Many processed foods Monosodium glutamate (MSG) hydrolyzed soy protein autolyzed yeast extract disodium guanylate disodium inosinate

N

NNH

N

O

OO

OHHO

OP

HOHO

NutrientsReplace vitamins and minerals lost in processing (enrichment)Add nutrients that may be lacking in the diet (fortification)

Flour breads cereals rice macaroni margarine salt milk fruit beverages energy bars instant breakfast drinks

Thiamine hydrochloride riboflavin (vitamin B2) niacin niacinamide folate or folic acid beta carotene alpha tocopherols ascorbic acid vitamin D Amino acids (L-tryptophan L-lysine L-leucine L-methionine)

H3C

H3C

HO

HO OH

OHO

O

NN

N NH

EmulsifiersAllow smooth mixing of ingredients prevent separationKeep emulsified products stableReduce stickinessControl crystallizationKeep ingredients dispersedHelp products dissolve more easily

Salad dressings peanut butter chocolate margarine frozen desserts

Soy lecithin mono- and diglycerides egg yolks polysorbates sorbitan monostearate

OOndash

P N+

O

O

OO

O

OR1

R2

CH3

CH3CH3

pH control agents and acidulantsControl acidity and alkalinityPrevent spoilage

Beverages frozen desserts chocolate baking powder

Lactic acid citric acid ammonium hydroxide sodium carbonate

infomn-netcom wwwmn-netcom4 5

Typical samples preparation 5rsquo-Mononucleotides in infant formula and adult pediatric nutritional formulaSample pretreatmentbullAccurately weigh approximately 1 g powder or 10 mL liquid

milk or ready-to-feed infant nutritional formula into a 50 mL centrifuge tube

bullDissolve in 30 mL extraction solution (NaCl 1 molL ndash EDTA 5 mmolL)

bullAdd 10 mL TMP intermediate standard (~80 μgmL)bullCap the tube and vortex mixbullAllow sample to stand for 10 min to ensure complete hydra-

tionbullDilute to a final volume of 50 mL with waterbullCap the tube and vortex mix

Solid phase extraction (SPE)MN AAp MNo 305960

BD Gill et al Journal of AOAC International Vol 95 No 3 2012 1ndash4

c Column type CHROMABONDreg SB 6 mL 1000 mg REF 730323

Note Throughout the extraction procedure do not let the column run dry but drain to the top of the column bed only When draining the column the flow rate should be lt 2 mLminColumn conditioning 4 mL methanol then 2 x 5 mL waterSample aspiration 4 mL sample solutionWashing 4 mL KBr (03 molL)Elution 4 mL KH2PO4 (05 molL pH 30)Compounds investigated uridine 5prime-monophosphate (UMP) inosine 5prime-monophosphate (IMP) adenosine 5prime-monophosphate (AMP) guanosine 5prime-monophosphate (GMP) cytidine 5prime-phosphate (CMP) thy-midine 5prime-monophosphate (TMP internal standard)

CHROMABONDreg vacuum manifold for 12 columns REF 730150

Transfer into a sample vial eg 15 mL screw neck vials N 9 amber flat bottom label and scale wide opening REF 702284 and ready assembled screw closures N 9 blue center hole with septum silicone PTFE REF 7022871

116

x 3

2 m

m

It is recommended to filter the prepared sample through dis-posable filters CHROMAFILreg Xtra PET-4525 REF 729220

For other applications see wwwmn-netcomaAAs

infomn-netcom wwwmn-netcom4 5

Analysis Subsequent analysis HPLCSynthetic antioxidants

MN AAp MNo 126980Column EC 1004 NUCLEOSHELLreg RP 18plus 27 μm

REF 76323440

0 5 10 15 20 min

1

2

3

Peaks1 Butylhydroxyanisole

HO

CH3

CH3

CH3

H3C O

3

2

2 Dodecyl gallate O

O CH3

OH

HO

HO

3 Butylhydroxytoluene

HOCH3

CH3

CH3

CH3

H3CH3C

H3C

Energy drink componentsMN AAp MNo 125010

Column EC 1004 NUCLEOSHELLreg HILIC 27 μm REF 76333440

0 1 min

1

2

3

4

5

6

Peaks1 Caffeine O

OCH3

CH3H3C N

N N

N

2 Niacinamide O

NH2N

3 PyridoxineOH

HO

CH3N

OH

4 Benzoic acidOH

O

5 Sorbic acid

H3C

O

OH

6 Riboflavin see page 3

Detailed conditions are available online at wwwmn-netcomaAAs

infomn-netcom wwwmn-netcom6 7

Analysis Subsequent analysis HPLCFood dyes (red and yellow dye mixtures)

MN AAp MNo 122500122510Column EC 2504 NUCLEODURreg C18 Gravity 5 μm

REF 76010140

1 2

3

4

5

6

7

Appl122500

0 5 10 min

1

23

45

Appl122510

PeaksRed dye mixture1 Ponceau 6R (E126)2 Ponceau 4R (E124)3 Azorubine (E122)4 Erythrosine (E127)5 Fast red EYellow dye mixture1 2 Tartrazine (E 126)3 Fast yellow4-6 Quinoline yellow (E 104)

NH

O

O(NaO3S)n

7 Yellow orange S (sunset yellow CFC E 110)

Ultra-fast separation of preservativesMN AAp MNo 123180

Column EC 504 NUCLEODURreg C8 Gravity 18 μm REF 76075540 coupled with EC 504 NUCLEODURreg C18 Gravity 18 μm REF 76007940

0 1

12

4

5 6 7

8 9

3

2 3 4 5 6 7 8 min

Peaks1 Salicylic acid (0056 mgmL)2 Benzoic acid (0052 mgmL)3 Sorbic acid (0049 mgmL)4 PHB phenoxy ester (0105 mgmL)5 PHB methyl ester (0025 mgmL)6 PHB ethyl ester (0026 mgmL)7 PHB propyl ester (0027 mgmL)8 PHB iso-butyl ester (0025 mgmL)9 PHB n-butyl ester (0025 mgmL)

Detailed conditions are available online at wwwmn-netcomaAAs

MN suggests using guard columns to protect your main column and significantly increase column life

The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings

OH

ORO

PHB esters

infomn-netcom wwwmn-netcom6 7

Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg SB 6 mL 1000 mg 30 730323

CHROMABONDreg vacuum manifold for 12 columns 1 730150Filtration Disposable filters CHROMAFILreg Xtra PET-4525 100 729220Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284

Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red

100 7022871

HPLC 1Column EC 1004 NUCLEOSHELLreg RP 18plus 27 μm 1 76323440Guard column EC 43 NUCLEOSHELLreg RP 18plus 27 μm 3 76323830Guard column holder Column Protection System 1 718966HPLC 2Column EC 1004 NUCLEOSHELLreg HILIC 27 μm 1 76333440Guard column EC 43 NUCLEOSHELLreg HILIC 27 μm 2 76333830Guard column holder Column Protection System 1 718966HPLC 3Column EC 2504 NUCLEODURreg C18 Gravity 5 μm 1 76010140Guard column EC 43 NUCLEODURreg C18 Gravity 5 μm 3 76190330Guard column holder Column Protection System 1 718966HPLC 4Column EC 504 NUCLEODURreg C8 Gravity 18 μm 1 76075540

EC 504 NUCLEODURreg C18 Gravity 18 μm 1 76007940Guard column EC 43 NUCLEODURreg C18 Gravity 18 μm 3 76190130Guard column holder Column Protection System 1 718966

Visit us at wwwmn-netcomchroma to get more helpful information

Selection tools for

Vials and caps

Syringe filters

Troubleshooting guidesGC and HPLC

Detailed product information

hellipand much more

wwwmn-netcom

wwwmn-netcomMACHEREY-NAGEL

EN ISO 9001 2008CERTIFIED

Food safety made easy with products from

MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

KATE

N20

0124

Foc

us F

lyer

Foo

d Vo

l 8

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

m

anla

ndash fo

tolia

com

infomn-netcom wwwmn-netcom2 3

Focus on additives The global production and trading of food and feed products makes monitoring and control difficult

Only analysis of food and raw materials for the production of food can ensure safe and edible food Food safety is an essential part of our quality of life

MN shows examples for food analysis that will help you with your work and make our food safer

Vol 8 Food additives

Food stuffs are frequently supplemented with additives in order to regulate or stabilize flavor color shelf life nutritional value and texture Food additives also ensure a smooth production process [FDA ndash httpwwwfdagovFoodIngredientsPackagingLabelingFoodAdditivesIngredi-entsucm094211htm]

Within the EU food additives are regulated by the Euro-pean Food Safety Authority (EFSA)

Outside the EU a Joint Expert Committee on Food Ad-ditives (JECFA) has been established by the Food and Agriculture Organization (FAO) and the World Health Or-ganization (WHO) [FAO ndash httpwwwfaoorgfoodfood-safe-ty-qualityscientific-advicejecfaen]

infomn-netcom wwwmn-netcom2 3

Compounds of interestOverview of selected food additives according to FDA

Types of ingredients Purpose function

Examples of uses Examples Typical molecules

Color additivesOffset color loss due to exposure to light air temperature extremes moisture and storage conditionsCorrect natural variations in colorEnhance colors that occur naturallyProvide color to colorless and ldquofunrdquo foods

Many processed foods eg candies snack foods margarine cheese soft drinks jams jellies gelatins pudding and pie fillings

FDampC Blue Nos 1 and 2 FDampC Yellow Nos 5 and 6 Orange B Ponceau 4R Beta-carotene (Note Exempt color additives are not required to be declared by name on labels but may be declared simply as colorings or color added)

N N

SO3ndash Na+

SO3ndash Na+

SO3ndash Na+

HO

Flavor enhancersEnhance flavors already present in foods (without providing their own separate flavor)

Many processed foods Monosodium glutamate (MSG) hydrolyzed soy protein autolyzed yeast extract disodium guanylate disodium inosinate

N

NNH

N

O

OO

OHHO

OP

HOHO

NutrientsReplace vitamins and minerals lost in processing (enrichment)Add nutrients that may be lacking in the diet (fortification)

Flour breads cereals rice macaroni margarine salt milk fruit beverages energy bars instant breakfast drinks

Thiamine hydrochloride riboflavin (vitamin B2) niacin niacinamide folate or folic acid beta carotene alpha tocopherols ascorbic acid vitamin D Amino acids (L-tryptophan L-lysine L-leucine L-methionine)

H3C

H3C

HO

HO OH

OHO

O

NN

N NH

EmulsifiersAllow smooth mixing of ingredients prevent separationKeep emulsified products stableReduce stickinessControl crystallizationKeep ingredients dispersedHelp products dissolve more easily

Salad dressings peanut butter chocolate margarine frozen desserts

Soy lecithin mono- and diglycerides egg yolks polysorbates sorbitan monostearate

OOndash

P N+

O

O

OO

O

OR1

R2

CH3

CH3CH3

pH control agents and acidulantsControl acidity and alkalinityPrevent spoilage

Beverages frozen desserts chocolate baking powder

Lactic acid citric acid ammonium hydroxide sodium carbonate

infomn-netcom wwwmn-netcom4 5

Typical samples preparation 5rsquo-Mononucleotides in infant formula and adult pediatric nutritional formulaSample pretreatmentbullAccurately weigh approximately 1 g powder or 10 mL liquid

milk or ready-to-feed infant nutritional formula into a 50 mL centrifuge tube

bullDissolve in 30 mL extraction solution (NaCl 1 molL ndash EDTA 5 mmolL)

bullAdd 10 mL TMP intermediate standard (~80 μgmL)bullCap the tube and vortex mixbullAllow sample to stand for 10 min to ensure complete hydra-

tionbullDilute to a final volume of 50 mL with waterbullCap the tube and vortex mix

Solid phase extraction (SPE)MN AAp MNo 305960

BD Gill et al Journal of AOAC International Vol 95 No 3 2012 1ndash4

c Column type CHROMABONDreg SB 6 mL 1000 mg REF 730323

Note Throughout the extraction procedure do not let the column run dry but drain to the top of the column bed only When draining the column the flow rate should be lt 2 mLminColumn conditioning 4 mL methanol then 2 x 5 mL waterSample aspiration 4 mL sample solutionWashing 4 mL KBr (03 molL)Elution 4 mL KH2PO4 (05 molL pH 30)Compounds investigated uridine 5prime-monophosphate (UMP) inosine 5prime-monophosphate (IMP) adenosine 5prime-monophosphate (AMP) guanosine 5prime-monophosphate (GMP) cytidine 5prime-phosphate (CMP) thy-midine 5prime-monophosphate (TMP internal standard)

CHROMABONDreg vacuum manifold for 12 columns REF 730150

Transfer into a sample vial eg 15 mL screw neck vials N 9 amber flat bottom label and scale wide opening REF 702284 and ready assembled screw closures N 9 blue center hole with septum silicone PTFE REF 7022871

116

x 3

2 m

m

It is recommended to filter the prepared sample through dis-posable filters CHROMAFILreg Xtra PET-4525 REF 729220

For other applications see wwwmn-netcomaAAs

infomn-netcom wwwmn-netcom4 5

Analysis Subsequent analysis HPLCSynthetic antioxidants

MN AAp MNo 126980Column EC 1004 NUCLEOSHELLreg RP 18plus 27 μm

REF 76323440

0 5 10 15 20 min

1

2

3

Peaks1 Butylhydroxyanisole

HO

CH3

CH3

CH3

H3C O

3

2

2 Dodecyl gallate O

O CH3

OH

HO

HO

3 Butylhydroxytoluene

HOCH3

CH3

CH3

CH3

H3CH3C

H3C

Energy drink componentsMN AAp MNo 125010

Column EC 1004 NUCLEOSHELLreg HILIC 27 μm REF 76333440

0 1 min

1

2

3

4

5

6

Peaks1 Caffeine O

OCH3

CH3H3C N

N N

N

2 Niacinamide O

NH2N

3 PyridoxineOH

HO

CH3N

OH

4 Benzoic acidOH

O

5 Sorbic acid

H3C

O

OH

6 Riboflavin see page 3

Detailed conditions are available online at wwwmn-netcomaAAs

infomn-netcom wwwmn-netcom6 7

Analysis Subsequent analysis HPLCFood dyes (red and yellow dye mixtures)

MN AAp MNo 122500122510Column EC 2504 NUCLEODURreg C18 Gravity 5 μm

REF 76010140

1 2

3

4

5

6

7

Appl122500

0 5 10 min

1

23

45

Appl122510

PeaksRed dye mixture1 Ponceau 6R (E126)2 Ponceau 4R (E124)3 Azorubine (E122)4 Erythrosine (E127)5 Fast red EYellow dye mixture1 2 Tartrazine (E 126)3 Fast yellow4-6 Quinoline yellow (E 104)

NH

O

O(NaO3S)n

7 Yellow orange S (sunset yellow CFC E 110)

Ultra-fast separation of preservativesMN AAp MNo 123180

Column EC 504 NUCLEODURreg C8 Gravity 18 μm REF 76075540 coupled with EC 504 NUCLEODURreg C18 Gravity 18 μm REF 76007940

0 1

12

4

5 6 7

8 9

3

2 3 4 5 6 7 8 min

Peaks1 Salicylic acid (0056 mgmL)2 Benzoic acid (0052 mgmL)3 Sorbic acid (0049 mgmL)4 PHB phenoxy ester (0105 mgmL)5 PHB methyl ester (0025 mgmL)6 PHB ethyl ester (0026 mgmL)7 PHB propyl ester (0027 mgmL)8 PHB iso-butyl ester (0025 mgmL)9 PHB n-butyl ester (0025 mgmL)

Detailed conditions are available online at wwwmn-netcomaAAs

MN suggests using guard columns to protect your main column and significantly increase column life

The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings

OH

ORO

PHB esters

infomn-netcom wwwmn-netcom6 7

Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg SB 6 mL 1000 mg 30 730323

CHROMABONDreg vacuum manifold for 12 columns 1 730150Filtration Disposable filters CHROMAFILreg Xtra PET-4525 100 729220Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284

Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red

100 7022871

HPLC 1Column EC 1004 NUCLEOSHELLreg RP 18plus 27 μm 1 76323440Guard column EC 43 NUCLEOSHELLreg RP 18plus 27 μm 3 76323830Guard column holder Column Protection System 1 718966HPLC 2Column EC 1004 NUCLEOSHELLreg HILIC 27 μm 1 76333440Guard column EC 43 NUCLEOSHELLreg HILIC 27 μm 2 76333830Guard column holder Column Protection System 1 718966HPLC 3Column EC 2504 NUCLEODURreg C18 Gravity 5 μm 1 76010140Guard column EC 43 NUCLEODURreg C18 Gravity 5 μm 3 76190330Guard column holder Column Protection System 1 718966HPLC 4Column EC 504 NUCLEODURreg C8 Gravity 18 μm 1 76075540

EC 504 NUCLEODURreg C18 Gravity 18 μm 1 76007940Guard column EC 43 NUCLEODURreg C18 Gravity 18 μm 3 76190130Guard column holder Column Protection System 1 718966

Visit us at wwwmn-netcomchroma to get more helpful information

Selection tools for

Vials and caps

Syringe filters

Troubleshooting guidesGC and HPLC

Detailed product information

hellipand much more

wwwmn-netcom

wwwmn-netcomMACHEREY-NAGEL

EN ISO 9001 2008CERTIFIED

Food safety made easy with products from

MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

KATE

N20

0124

Foc

us F

lyer

Foo

d Vo

l 8

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

m

anla

ndash fo

tolia

com

infomn-netcom wwwmn-netcom2 3

Compounds of interestOverview of selected food additives according to FDA

Types of ingredients Purpose function

Examples of uses Examples Typical molecules

Color additivesOffset color loss due to exposure to light air temperature extremes moisture and storage conditionsCorrect natural variations in colorEnhance colors that occur naturallyProvide color to colorless and ldquofunrdquo foods

Many processed foods eg candies snack foods margarine cheese soft drinks jams jellies gelatins pudding and pie fillings

FDampC Blue Nos 1 and 2 FDampC Yellow Nos 5 and 6 Orange B Ponceau 4R Beta-carotene (Note Exempt color additives are not required to be declared by name on labels but may be declared simply as colorings or color added)

N N

SO3ndash Na+

SO3ndash Na+

SO3ndash Na+

HO

Flavor enhancersEnhance flavors already present in foods (without providing their own separate flavor)

Many processed foods Monosodium glutamate (MSG) hydrolyzed soy protein autolyzed yeast extract disodium guanylate disodium inosinate

N

NNH

N

O

OO

OHHO

OP

HOHO

NutrientsReplace vitamins and minerals lost in processing (enrichment)Add nutrients that may be lacking in the diet (fortification)

Flour breads cereals rice macaroni margarine salt milk fruit beverages energy bars instant breakfast drinks

Thiamine hydrochloride riboflavin (vitamin B2) niacin niacinamide folate or folic acid beta carotene alpha tocopherols ascorbic acid vitamin D Amino acids (L-tryptophan L-lysine L-leucine L-methionine)

H3C

H3C

HO

HO OH

OHO

O

NN

N NH

EmulsifiersAllow smooth mixing of ingredients prevent separationKeep emulsified products stableReduce stickinessControl crystallizationKeep ingredients dispersedHelp products dissolve more easily

Salad dressings peanut butter chocolate margarine frozen desserts

Soy lecithin mono- and diglycerides egg yolks polysorbates sorbitan monostearate

OOndash

P N+

O

O

OO

O

OR1

R2

CH3

CH3CH3

pH control agents and acidulantsControl acidity and alkalinityPrevent spoilage

Beverages frozen desserts chocolate baking powder

Lactic acid citric acid ammonium hydroxide sodium carbonate

infomn-netcom wwwmn-netcom4 5

Typical samples preparation 5rsquo-Mononucleotides in infant formula and adult pediatric nutritional formulaSample pretreatmentbullAccurately weigh approximately 1 g powder or 10 mL liquid

milk or ready-to-feed infant nutritional formula into a 50 mL centrifuge tube

bullDissolve in 30 mL extraction solution (NaCl 1 molL ndash EDTA 5 mmolL)

bullAdd 10 mL TMP intermediate standard (~80 μgmL)bullCap the tube and vortex mixbullAllow sample to stand for 10 min to ensure complete hydra-

tionbullDilute to a final volume of 50 mL with waterbullCap the tube and vortex mix

Solid phase extraction (SPE)MN AAp MNo 305960

BD Gill et al Journal of AOAC International Vol 95 No 3 2012 1ndash4

c Column type CHROMABONDreg SB 6 mL 1000 mg REF 730323

Note Throughout the extraction procedure do not let the column run dry but drain to the top of the column bed only When draining the column the flow rate should be lt 2 mLminColumn conditioning 4 mL methanol then 2 x 5 mL waterSample aspiration 4 mL sample solutionWashing 4 mL KBr (03 molL)Elution 4 mL KH2PO4 (05 molL pH 30)Compounds investigated uridine 5prime-monophosphate (UMP) inosine 5prime-monophosphate (IMP) adenosine 5prime-monophosphate (AMP) guanosine 5prime-monophosphate (GMP) cytidine 5prime-phosphate (CMP) thy-midine 5prime-monophosphate (TMP internal standard)

CHROMABONDreg vacuum manifold for 12 columns REF 730150

Transfer into a sample vial eg 15 mL screw neck vials N 9 amber flat bottom label and scale wide opening REF 702284 and ready assembled screw closures N 9 blue center hole with septum silicone PTFE REF 7022871

116

x 3

2 m

m

It is recommended to filter the prepared sample through dis-posable filters CHROMAFILreg Xtra PET-4525 REF 729220

For other applications see wwwmn-netcomaAAs

infomn-netcom wwwmn-netcom4 5

Analysis Subsequent analysis HPLCSynthetic antioxidants

MN AAp MNo 126980Column EC 1004 NUCLEOSHELLreg RP 18plus 27 μm

REF 76323440

0 5 10 15 20 min

1

2

3

Peaks1 Butylhydroxyanisole

HO

CH3

CH3

CH3

H3C O

3

2

2 Dodecyl gallate O

O CH3

OH

HO

HO

3 Butylhydroxytoluene

HOCH3

CH3

CH3

CH3

H3CH3C

H3C

Energy drink componentsMN AAp MNo 125010

Column EC 1004 NUCLEOSHELLreg HILIC 27 μm REF 76333440

0 1 min

1

2

3

4

5

6

Peaks1 Caffeine O

OCH3

CH3H3C N

N N

N

2 Niacinamide O

NH2N

3 PyridoxineOH

HO

CH3N

OH

4 Benzoic acidOH

O

5 Sorbic acid

H3C

O

OH

6 Riboflavin see page 3

Detailed conditions are available online at wwwmn-netcomaAAs

infomn-netcom wwwmn-netcom6 7

Analysis Subsequent analysis HPLCFood dyes (red and yellow dye mixtures)

MN AAp MNo 122500122510Column EC 2504 NUCLEODURreg C18 Gravity 5 μm

REF 76010140

1 2

3

4

5

6

7

Appl122500

0 5 10 min

1

23

45

Appl122510

PeaksRed dye mixture1 Ponceau 6R (E126)2 Ponceau 4R (E124)3 Azorubine (E122)4 Erythrosine (E127)5 Fast red EYellow dye mixture1 2 Tartrazine (E 126)3 Fast yellow4-6 Quinoline yellow (E 104)

NH

O

O(NaO3S)n

7 Yellow orange S (sunset yellow CFC E 110)

Ultra-fast separation of preservativesMN AAp MNo 123180

Column EC 504 NUCLEODURreg C8 Gravity 18 μm REF 76075540 coupled with EC 504 NUCLEODURreg C18 Gravity 18 μm REF 76007940

0 1

12

4

5 6 7

8 9

3

2 3 4 5 6 7 8 min

Peaks1 Salicylic acid (0056 mgmL)2 Benzoic acid (0052 mgmL)3 Sorbic acid (0049 mgmL)4 PHB phenoxy ester (0105 mgmL)5 PHB methyl ester (0025 mgmL)6 PHB ethyl ester (0026 mgmL)7 PHB propyl ester (0027 mgmL)8 PHB iso-butyl ester (0025 mgmL)9 PHB n-butyl ester (0025 mgmL)

Detailed conditions are available online at wwwmn-netcomaAAs

MN suggests using guard columns to protect your main column and significantly increase column life

The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings

OH

ORO

PHB esters

infomn-netcom wwwmn-netcom6 7

Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg SB 6 mL 1000 mg 30 730323

CHROMABONDreg vacuum manifold for 12 columns 1 730150Filtration Disposable filters CHROMAFILreg Xtra PET-4525 100 729220Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284

Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red

100 7022871

HPLC 1Column EC 1004 NUCLEOSHELLreg RP 18plus 27 μm 1 76323440Guard column EC 43 NUCLEOSHELLreg RP 18plus 27 μm 3 76323830Guard column holder Column Protection System 1 718966HPLC 2Column EC 1004 NUCLEOSHELLreg HILIC 27 μm 1 76333440Guard column EC 43 NUCLEOSHELLreg HILIC 27 μm 2 76333830Guard column holder Column Protection System 1 718966HPLC 3Column EC 2504 NUCLEODURreg C18 Gravity 5 μm 1 76010140Guard column EC 43 NUCLEODURreg C18 Gravity 5 μm 3 76190330Guard column holder Column Protection System 1 718966HPLC 4Column EC 504 NUCLEODURreg C8 Gravity 18 μm 1 76075540

EC 504 NUCLEODURreg C18 Gravity 18 μm 1 76007940Guard column EC 43 NUCLEODURreg C18 Gravity 18 μm 3 76190130Guard column holder Column Protection System 1 718966

Visit us at wwwmn-netcomchroma to get more helpful information

Selection tools for

Vials and caps

Syringe filters

Troubleshooting guidesGC and HPLC

Detailed product information

hellipand much more

wwwmn-netcom

wwwmn-netcomMACHEREY-NAGEL

EN ISO 9001 2008CERTIFIED

Food safety made easy with products from

MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

KATE

N20

0124

Foc

us F

lyer

Foo

d Vo

l 8

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

m

anla

ndash fo

tolia

com

infomn-netcom wwwmn-netcom4 5

Typical samples preparation 5rsquo-Mononucleotides in infant formula and adult pediatric nutritional formulaSample pretreatmentbullAccurately weigh approximately 1 g powder or 10 mL liquid

milk or ready-to-feed infant nutritional formula into a 50 mL centrifuge tube

bullDissolve in 30 mL extraction solution (NaCl 1 molL ndash EDTA 5 mmolL)

bullAdd 10 mL TMP intermediate standard (~80 μgmL)bullCap the tube and vortex mixbullAllow sample to stand for 10 min to ensure complete hydra-

tionbullDilute to a final volume of 50 mL with waterbullCap the tube and vortex mix

Solid phase extraction (SPE)MN AAp MNo 305960

BD Gill et al Journal of AOAC International Vol 95 No 3 2012 1ndash4

c Column type CHROMABONDreg SB 6 mL 1000 mg REF 730323

Note Throughout the extraction procedure do not let the column run dry but drain to the top of the column bed only When draining the column the flow rate should be lt 2 mLminColumn conditioning 4 mL methanol then 2 x 5 mL waterSample aspiration 4 mL sample solutionWashing 4 mL KBr (03 molL)Elution 4 mL KH2PO4 (05 molL pH 30)Compounds investigated uridine 5prime-monophosphate (UMP) inosine 5prime-monophosphate (IMP) adenosine 5prime-monophosphate (AMP) guanosine 5prime-monophosphate (GMP) cytidine 5prime-phosphate (CMP) thy-midine 5prime-monophosphate (TMP internal standard)

CHROMABONDreg vacuum manifold for 12 columns REF 730150

Transfer into a sample vial eg 15 mL screw neck vials N 9 amber flat bottom label and scale wide opening REF 702284 and ready assembled screw closures N 9 blue center hole with septum silicone PTFE REF 7022871

116

x 3

2 m

m

It is recommended to filter the prepared sample through dis-posable filters CHROMAFILreg Xtra PET-4525 REF 729220

For other applications see wwwmn-netcomaAAs

infomn-netcom wwwmn-netcom4 5

Analysis Subsequent analysis HPLCSynthetic antioxidants

MN AAp MNo 126980Column EC 1004 NUCLEOSHELLreg RP 18plus 27 μm

REF 76323440

0 5 10 15 20 min

1

2

3

Peaks1 Butylhydroxyanisole

HO

CH3

CH3

CH3

H3C O

3

2

2 Dodecyl gallate O

O CH3

OH

HO

HO

3 Butylhydroxytoluene

HOCH3

CH3

CH3

CH3

H3CH3C

H3C

Energy drink componentsMN AAp MNo 125010

Column EC 1004 NUCLEOSHELLreg HILIC 27 μm REF 76333440

0 1 min

1

2

3

4

5

6

Peaks1 Caffeine O

OCH3

CH3H3C N

N N

N

2 Niacinamide O

NH2N

3 PyridoxineOH

HO

CH3N

OH

4 Benzoic acidOH

O

5 Sorbic acid

H3C

O

OH

6 Riboflavin see page 3

Detailed conditions are available online at wwwmn-netcomaAAs

infomn-netcom wwwmn-netcom6 7

Analysis Subsequent analysis HPLCFood dyes (red and yellow dye mixtures)

MN AAp MNo 122500122510Column EC 2504 NUCLEODURreg C18 Gravity 5 μm

REF 76010140

1 2

3

4

5

6

7

Appl122500

0 5 10 min

1

23

45

Appl122510

PeaksRed dye mixture1 Ponceau 6R (E126)2 Ponceau 4R (E124)3 Azorubine (E122)4 Erythrosine (E127)5 Fast red EYellow dye mixture1 2 Tartrazine (E 126)3 Fast yellow4-6 Quinoline yellow (E 104)

NH

O

O(NaO3S)n

7 Yellow orange S (sunset yellow CFC E 110)

Ultra-fast separation of preservativesMN AAp MNo 123180

Column EC 504 NUCLEODURreg C8 Gravity 18 μm REF 76075540 coupled with EC 504 NUCLEODURreg C18 Gravity 18 μm REF 76007940

0 1

12

4

5 6 7

8 9

3

2 3 4 5 6 7 8 min

Peaks1 Salicylic acid (0056 mgmL)2 Benzoic acid (0052 mgmL)3 Sorbic acid (0049 mgmL)4 PHB phenoxy ester (0105 mgmL)5 PHB methyl ester (0025 mgmL)6 PHB ethyl ester (0026 mgmL)7 PHB propyl ester (0027 mgmL)8 PHB iso-butyl ester (0025 mgmL)9 PHB n-butyl ester (0025 mgmL)

Detailed conditions are available online at wwwmn-netcomaAAs

MN suggests using guard columns to protect your main column and significantly increase column life

The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings

OH

ORO

PHB esters

infomn-netcom wwwmn-netcom6 7

Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg SB 6 mL 1000 mg 30 730323

CHROMABONDreg vacuum manifold for 12 columns 1 730150Filtration Disposable filters CHROMAFILreg Xtra PET-4525 100 729220Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284

Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red

100 7022871

HPLC 1Column EC 1004 NUCLEOSHELLreg RP 18plus 27 μm 1 76323440Guard column EC 43 NUCLEOSHELLreg RP 18plus 27 μm 3 76323830Guard column holder Column Protection System 1 718966HPLC 2Column EC 1004 NUCLEOSHELLreg HILIC 27 μm 1 76333440Guard column EC 43 NUCLEOSHELLreg HILIC 27 μm 2 76333830Guard column holder Column Protection System 1 718966HPLC 3Column EC 2504 NUCLEODURreg C18 Gravity 5 μm 1 76010140Guard column EC 43 NUCLEODURreg C18 Gravity 5 μm 3 76190330Guard column holder Column Protection System 1 718966HPLC 4Column EC 504 NUCLEODURreg C8 Gravity 18 μm 1 76075540

EC 504 NUCLEODURreg C18 Gravity 18 μm 1 76007940Guard column EC 43 NUCLEODURreg C18 Gravity 18 μm 3 76190130Guard column holder Column Protection System 1 718966

Visit us at wwwmn-netcomchroma to get more helpful information

Selection tools for

Vials and caps

Syringe filters

Troubleshooting guidesGC and HPLC

Detailed product information

hellipand much more

wwwmn-netcom

wwwmn-netcomMACHEREY-NAGEL

EN ISO 9001 2008CERTIFIED

Food safety made easy with products from

MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

KATE

N20

0124

Foc

us F

lyer

Foo

d Vo

l 8

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

m

anla

ndash fo

tolia

com

infomn-netcom wwwmn-netcom4 5

Analysis Subsequent analysis HPLCSynthetic antioxidants

MN AAp MNo 126980Column EC 1004 NUCLEOSHELLreg RP 18plus 27 μm

REF 76323440

0 5 10 15 20 min

1

2

3

Peaks1 Butylhydroxyanisole

HO

CH3

CH3

CH3

H3C O

3

2

2 Dodecyl gallate O

O CH3

OH

HO

HO

3 Butylhydroxytoluene

HOCH3

CH3

CH3

CH3

H3CH3C

H3C

Energy drink componentsMN AAp MNo 125010

Column EC 1004 NUCLEOSHELLreg HILIC 27 μm REF 76333440

0 1 min

1

2

3

4

5

6

Peaks1 Caffeine O

OCH3

CH3H3C N

N N

N

2 Niacinamide O

NH2N

3 PyridoxineOH

HO

CH3N

OH

4 Benzoic acidOH

O

5 Sorbic acid

H3C

O

OH

6 Riboflavin see page 3

Detailed conditions are available online at wwwmn-netcomaAAs

infomn-netcom wwwmn-netcom6 7

Analysis Subsequent analysis HPLCFood dyes (red and yellow dye mixtures)

MN AAp MNo 122500122510Column EC 2504 NUCLEODURreg C18 Gravity 5 μm

REF 76010140

1 2

3

4

5

6

7

Appl122500

0 5 10 min

1

23

45

Appl122510

PeaksRed dye mixture1 Ponceau 6R (E126)2 Ponceau 4R (E124)3 Azorubine (E122)4 Erythrosine (E127)5 Fast red EYellow dye mixture1 2 Tartrazine (E 126)3 Fast yellow4-6 Quinoline yellow (E 104)

NH

O

O(NaO3S)n

7 Yellow orange S (sunset yellow CFC E 110)

Ultra-fast separation of preservativesMN AAp MNo 123180

Column EC 504 NUCLEODURreg C8 Gravity 18 μm REF 76075540 coupled with EC 504 NUCLEODURreg C18 Gravity 18 μm REF 76007940

0 1

12

4

5 6 7

8 9

3

2 3 4 5 6 7 8 min

Peaks1 Salicylic acid (0056 mgmL)2 Benzoic acid (0052 mgmL)3 Sorbic acid (0049 mgmL)4 PHB phenoxy ester (0105 mgmL)5 PHB methyl ester (0025 mgmL)6 PHB ethyl ester (0026 mgmL)7 PHB propyl ester (0027 mgmL)8 PHB iso-butyl ester (0025 mgmL)9 PHB n-butyl ester (0025 mgmL)

Detailed conditions are available online at wwwmn-netcomaAAs

MN suggests using guard columns to protect your main column and significantly increase column life

The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings

OH

ORO

PHB esters

infomn-netcom wwwmn-netcom6 7

Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg SB 6 mL 1000 mg 30 730323

CHROMABONDreg vacuum manifold for 12 columns 1 730150Filtration Disposable filters CHROMAFILreg Xtra PET-4525 100 729220Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284

Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red

100 7022871

HPLC 1Column EC 1004 NUCLEOSHELLreg RP 18plus 27 μm 1 76323440Guard column EC 43 NUCLEOSHELLreg RP 18plus 27 μm 3 76323830Guard column holder Column Protection System 1 718966HPLC 2Column EC 1004 NUCLEOSHELLreg HILIC 27 μm 1 76333440Guard column EC 43 NUCLEOSHELLreg HILIC 27 μm 2 76333830Guard column holder Column Protection System 1 718966HPLC 3Column EC 2504 NUCLEODURreg C18 Gravity 5 μm 1 76010140Guard column EC 43 NUCLEODURreg C18 Gravity 5 μm 3 76190330Guard column holder Column Protection System 1 718966HPLC 4Column EC 504 NUCLEODURreg C8 Gravity 18 μm 1 76075540

EC 504 NUCLEODURreg C18 Gravity 18 μm 1 76007940Guard column EC 43 NUCLEODURreg C18 Gravity 18 μm 3 76190130Guard column holder Column Protection System 1 718966

Visit us at wwwmn-netcomchroma to get more helpful information

Selection tools for

Vials and caps

Syringe filters

Troubleshooting guidesGC and HPLC

Detailed product information

hellipand much more

wwwmn-netcom

wwwmn-netcomMACHEREY-NAGEL

EN ISO 9001 2008CERTIFIED

Food safety made easy with products from

MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

KATE

N20

0124

Foc

us F

lyer

Foo

d Vo

l 8

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

m

anla

ndash fo

tolia

com

infomn-netcom wwwmn-netcom6 7

Analysis Subsequent analysis HPLCFood dyes (red and yellow dye mixtures)

MN AAp MNo 122500122510Column EC 2504 NUCLEODURreg C18 Gravity 5 μm

REF 76010140

1 2

3

4

5

6

7

Appl122500

0 5 10 min

1

23

45

Appl122510

PeaksRed dye mixture1 Ponceau 6R (E126)2 Ponceau 4R (E124)3 Azorubine (E122)4 Erythrosine (E127)5 Fast red EYellow dye mixture1 2 Tartrazine (E 126)3 Fast yellow4-6 Quinoline yellow (E 104)

NH

O

O(NaO3S)n

7 Yellow orange S (sunset yellow CFC E 110)

Ultra-fast separation of preservativesMN AAp MNo 123180

Column EC 504 NUCLEODURreg C8 Gravity 18 μm REF 76075540 coupled with EC 504 NUCLEODURreg C18 Gravity 18 μm REF 76007940

0 1

12

4

5 6 7

8 9

3

2 3 4 5 6 7 8 min

Peaks1 Salicylic acid (0056 mgmL)2 Benzoic acid (0052 mgmL)3 Sorbic acid (0049 mgmL)4 PHB phenoxy ester (0105 mgmL)5 PHB methyl ester (0025 mgmL)6 PHB ethyl ester (0026 mgmL)7 PHB propyl ester (0027 mgmL)8 PHB iso-butyl ester (0025 mgmL)9 PHB n-butyl ester (0025 mgmL)

Detailed conditions are available online at wwwmn-netcomaAAs

MN suggests using guard columns to protect your main column and significantly increase column life

The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings

OH

ORO

PHB esters

infomn-netcom wwwmn-netcom6 7

Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg SB 6 mL 1000 mg 30 730323

CHROMABONDreg vacuum manifold for 12 columns 1 730150Filtration Disposable filters CHROMAFILreg Xtra PET-4525 100 729220Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284

Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red

100 7022871

HPLC 1Column EC 1004 NUCLEOSHELLreg RP 18plus 27 μm 1 76323440Guard column EC 43 NUCLEOSHELLreg RP 18plus 27 μm 3 76323830Guard column holder Column Protection System 1 718966HPLC 2Column EC 1004 NUCLEOSHELLreg HILIC 27 μm 1 76333440Guard column EC 43 NUCLEOSHELLreg HILIC 27 μm 2 76333830Guard column holder Column Protection System 1 718966HPLC 3Column EC 2504 NUCLEODURreg C18 Gravity 5 μm 1 76010140Guard column EC 43 NUCLEODURreg C18 Gravity 5 μm 3 76190330Guard column holder Column Protection System 1 718966HPLC 4Column EC 504 NUCLEODURreg C8 Gravity 18 μm 1 76075540

EC 504 NUCLEODURreg C18 Gravity 18 μm 1 76007940Guard column EC 43 NUCLEODURreg C18 Gravity 18 μm 3 76190130Guard column holder Column Protection System 1 718966

Visit us at wwwmn-netcomchroma to get more helpful information

Selection tools for

Vials and caps

Syringe filters

Troubleshooting guidesGC and HPLC

Detailed product information

hellipand much more

wwwmn-netcom

wwwmn-netcomMACHEREY-NAGEL

EN ISO 9001 2008CERTIFIED

Food safety made easy with products from

MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

KATE

N20

0124

Foc

us F

lyer

Foo

d Vo

l 8

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

m

anla

ndash fo

tolia

com

infomn-netcom wwwmn-netcom6 7

Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg SB 6 mL 1000 mg 30 730323

CHROMABONDreg vacuum manifold for 12 columns 1 730150Filtration Disposable filters CHROMAFILreg Xtra PET-4525 100 729220Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284

Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red

100 7022871

HPLC 1Column EC 1004 NUCLEOSHELLreg RP 18plus 27 μm 1 76323440Guard column EC 43 NUCLEOSHELLreg RP 18plus 27 μm 3 76323830Guard column holder Column Protection System 1 718966HPLC 2Column EC 1004 NUCLEOSHELLreg HILIC 27 μm 1 76333440Guard column EC 43 NUCLEOSHELLreg HILIC 27 μm 2 76333830Guard column holder Column Protection System 1 718966HPLC 3Column EC 2504 NUCLEODURreg C18 Gravity 5 μm 1 76010140Guard column EC 43 NUCLEODURreg C18 Gravity 5 μm 3 76190330Guard column holder Column Protection System 1 718966HPLC 4Column EC 504 NUCLEODURreg C8 Gravity 18 μm 1 76075540

EC 504 NUCLEODURreg C18 Gravity 18 μm 1 76007940Guard column EC 43 NUCLEODURreg C18 Gravity 18 μm 3 76190130Guard column holder Column Protection System 1 718966

Visit us at wwwmn-netcomchroma to get more helpful information

Selection tools for

Vials and caps

Syringe filters

Troubleshooting guidesGC and HPLC

Detailed product information

hellipand much more

wwwmn-netcom

wwwmn-netcomMACHEREY-NAGEL

EN ISO 9001 2008CERTIFIED

Food safety made easy with products from

MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

KATE

N20

0124

Foc

us F

lyer

Foo

d Vo

l 8

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

m

anla

ndash fo

tolia

com

wwwmn-netcom

wwwmn-netcomMACHEREY-NAGEL

EN ISO 9001 2008CERTIFIED

Food safety made easy with products from

MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

KATE

N20

0124

Foc

us F

lyer

Foo

d Vo

l 8

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

m

anla

ndash fo

tolia

com