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Chris SkinnerEl Campo ISD
Zhilei Chen, PhD Artie McFerrin Department
of Chemical Engineering
Texas A&M University
Protein EngineeringDNA for the desired protein is identified,
and cut out with restriction enzymes.Plasmid DNA is cut with the same
restriction enzymes.The pieces are put together with an
enzyme called ligase to create a new plasmid.
Transformation and PurificationNewly engineered plasmid is then put into E.
coli through a process called transformation.Transformed E. coli is then grown and
induced to make the newly engineered enzyme.
•Once harvested, the proteins must be purified, or isolated
•During the engineering of the protein, it was "tagged" to make isolation easier
•The solution containing the protein is poured through a filter system
Purification (continued)• The protein is eluted from the filter using a buffer
that has a higher affinity for the molecule• The collected protein solution undergoes
electrophoresis to demonstrate its purity
Fuel Cell ConstructionMix enzyme with Multi-walled carbon nanotubes (MWNT’s)•Fix enzyme to electrodes•Argarose + MWNT + enzyme
STAAR/EOC OBJECTIVESP.1.A demonstrate safe practices during laboratory and
field investigationsP.1.B demonstrate an understanding of the use and
conservation of resources and the proper disposal or recycling of materials
P.2.A know the definition of science and understand that it has limitations, as specified in chapter 112.39, subsection (b)(2) of 19 TAC
P.2.B know that scientific hypotheses are tentative and testable statements that must be capable of being supported or not supported by observational evidence. Hypotheses of durable explanatory power which have been tested over a wide variety of conditions are incorporated into theories
STAAR/EOC OBJECTIVES (cont.)P.2.C know that scientific theories are based on natural and
physical phenomena and are capable of being tested by multiple independent researchers. Unlike hypotheses, scientific theories are well‐established and highly‐reliable explanations, but may be subject to change as new areas of science and new technologies are developed
P.2.D distinguish between scientific hypotheses and scientific theories
P.2.E design and implement investigative procedures, including making observations, asking well‐defined questions, formulating testable hypotheses, identifying variables, selecting appropriate equipment and technology, and evaluating numerical answers for reasonableness
P.2.H make measurements with accuracy and precision and record data using scientific notation and International System (SI) units
STAAR/EOC OBJECTIVES (cont.)P.2.I identify and quantify causes and effects of uncertainties in
measured dataP.2.J organize and evaluate data and make inferences from data,
including the use of tables, charts, and graphsP.2.K communicate valid conclusions supported by the data through
various methods such as lab reports, labeled drawings, graphicorganizers, journals, summaries, oral reports, and technology‐based
reportsP.2.L express and manipulate relationships among physical variables
quantitatively, including the use of graphs, charts, and equationsP.3.A in all fields of science, analyze, evaluate, and critique scientific
explanations by using empirical evidence, logical reasoning, and experimental and observational testing, including examining all sides of scientific evidence of those scientific explanations, so as to encourage critical thinking by the student
STAAR/EOC OBJECTIVES (cont.)P.3.B communicate and apply scientific information
extracted from various sources such as current events, news reports, published journal articles, and marketing materials
P.3.E research and describe the connections between physics and future careers
P.5.D identify examples of electric and magnetic forces in everyday life
P.5.E characterize materials as conductors or insulators based on their electrical properties
P.5.F design, construct, and calculate in terms of current through, potential difference across, resistance of, and power used by electric circuit elements connected in both series and parallel combinations.
Enzymes as an Alternative Fuel Source
Day 1Bacterial Reproduction
Guided Activty: Pglo DNA replication Genetic Engineering
Snail fuel video
Greenlaw, Mackinley, “DOD: Cyborg Snails Make Good Batteries, Online Video Clip, YouTube, March 14, 2012, June 25, 2012, http://www.youtube.com/watch?v=-RVaXjZ8MlQ
Enzymes as an Alternative Fuel Source
REDOX Reactions Reversible reactions Liberation of electrons
Electrical Circuit Anode Cathode Connecting wires (MWNT)
Pre-testPre-lab worksheet
Enzymes as an Alternative Fuel Source
Day 2Construction of control fuel cell
Glucose oxidase (Anode) Laccase (Cathode) Buffer
Construction of working fuel cell Glucose oxidase (Anode) Laccase (Cathode) Buffer MWNT’s
Engineering DesignDay 3 Engineering Design
1. Asking Questions vrs. Defining Problems2. Developing and Using Models3. Planning & Carrying Out Investigations4. Analyzing and Interpreting Data5. Using Mathematics and Computational Thinking6. Constructing Explanations and Designing Solutions7. Engaging in Argument From Evidence8. Obtaining, Evaluating and Communicating InformationSource: NAS, A Framework for K-12 Science Education
Engineering Design1. Define the Problem – In many cases, done for you by a client2. Brainstorm 3. Research and Generate Ideas – gather background information on
different aspects of the problem4. Identify Criteria, Constraints & Performance Specifications –
define what the system must do (compare to Design Specs)5. Explore Ideas and Invention6. Analysis & Selection – at this stage you have structured the problem,
and can now apply sophisticated analysis techniques to examine the performance of the design. You have some potentially viable designs (use categories Cost, Safety, Performance, Reliability to Rank each)
7. Develop Detailed Design8. Model or Prototyping and Testing9. Test & Evaluate10. Refine11. Production12. Communicate Results
AssessmentPresentation
Requirement Alottment
Data/Analysis 15
Incorporation of Engineering Design
30
Process improvement 40
Future application 5
Presentation/Design 10