ChIR Symposium 2017 Faro, 3-4 July - micoperibg.eu · ChIR Symposium 2017 Faro, 3-4 July Matilde Mazzotti (a), ... cyanobacteria Due to environmental competition and contamination

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ChIR Symposium 2017 Faro, 3-4 July

Matilde Mazzotti (a), Flavio Guidi(a) , Gianni Sulas(a) , Guido Emiliani (a)

(a) Micoperi Blue Growth srl, via Trieste 279 Ravenna

• Start up company born in 2014 • Develop new technologies to produce

sustainable algal biomass • Guarantee of traceability and high quality of

its products • Core business: research on microalgae

cultivations

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3. Micoperi Blue Growth :” A blue growth for a sustainable future”

2 (c) MBG 2017 - All right reserved

ChIR Symposium 2017

© MBG 2015 – All rights reserved

CONTENTS

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1. MICROALGAE

2. FIELD OF APPLICATION

3. MARKET

4. CULTIVATION..AND CONTAMINATION

5. INNOVATIVE STRATEGY

6. MISSING..

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ChIR Symposium 2017

1. WHY MICROALGAE?

4

NUCLEUS

LIPID DROPS

CHROMOPLAST

CHLOROPLAST

CELL WALL Pigments

Chlorophyll

Antioxidants

Carotenoids

Oil Omega 3

Biopolymers

β-Glucans

The biofactory


ChIR Symposium 2017

• Prokaryotic and eukaryotic • Wide range habitats • With diverse metabolic potential • Different organic compounds • Half of the O2 • CO2 sequestration

0

20

40

60

80

100

120

0 20 40 60 80 100 120

Air equilibrium

External CO2 concentration (mM) R

ate o

f C

ass

imilat

ion

(%

max

)

+CCM

-CCM

CO2 concentrating mechanisms (CCM)

1. WHY MICROALGAE?

Higher photosynthetic efficiency

More rapid biomass accumulation

than in land plants


ChIR Symposium 2017

Differences with land plants:

Greater ability to capture light because of Rubisco enzyme and to adapt their PSI and PSII

CCM facilitate CO2 uptake when there is low availability of it. Pumped CO2 near Rubisco: high speed and concentrations

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2. FIELDS OF APPLICATION


ChIR Symposium 2017

200s species:

• > 100.000 species identified • 200 species used in worldwide

sectors • Only 5 -6 species are today

commercially exploited

• Attractive for commercial exploitation in a low carbon economy

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Only DIETARY SUPPLEMENTS

(without anykind of processing except

drying)


ChIR Symposium 2017

• Exploited for centuries as food !

• Clorella e spirulina

• Full of bioactive compounds to become innovative source for new food !!

COMMERCIALISED!!

EARLY DEVELOPMENT!!

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VITAMIN B12 / 500 gr Meat

5 gr. Spirulina = 1 Spoon

PROTEIN / 35 gr Beef

IRON / 300 gr Spinach

CALCIUM / 3 glass of milk

pOTASSIUM / 1 tomato

PHOSPHOROUS / 1 anchovy

ß CAROTENE/ 18 carotts

VITAMIN E/ 3 almonds

ChIR Symposium 2017

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• Potential to ricover nutrients from waste and release feritlizer

• Induce systemic resisthance in plants

against pathogens (MBG’s PATENT for ISR)

ChIR Symposium 2017

The heightend awareness concerning environmental preservation and resource scarsity has produced the need for a more sustainable AGRICULTURAL production systems

• Several studies performed on pets, fishes and farm animals: animal health growth and nutriotional properties

• Source of protein, color, lipids….

COMMERCIALISED!!



High valuable compounds for :

PHARMACEUTICAL, MEDICAL AND

COSMETICS

EXTRACTS

ChIR Symposium 2017

Pigments: phicoerythrin, astaxanthin, phycocyanin, carotenoids..

PUFA’s: ARA, EPA, DHA,

Sulfated polysacharid, Beta-glucan, proteins….

Many species, intentionally stressed by specific factors (strong light, nutrients depletion, salt..) or during specific phase of growth can produce interesting compounds



Marker Anticancer, Antitumoral,

Antiviral, Antioxidant

ChIR Symposium 2017

Specific advantages over synthetic alternatives

Many EU producers are stepping in algae exctract!

C

O

S

M

E

T

I

C

anti-inflammation, anti-aging, hydrating, suncare

M

E

D

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A

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P

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COMMERCIALISED!!

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NH4+ : 97 µmol g-1 h-1

NO3-: 13 97 µmol g-1 h-1

Mazzotti et al. (in preparation)


ChIR Symposium 2017

• Treatment of watewater pollution

• nutrients and heavy metal removal

• Research since 1950 several wastewater and species

• Assimilation and bioaccumulation

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2. FIELDS OF APPLICATION Third generation of BIOFUELS

SOLUTION: INTEGRATED SYSTEMS!!!!!!!

EXPENSIVE!!

High PUFA’s

contents!!!


ChIR Symposium 2017

Attractive reasons for microalgae in biofuels: • Marginal lands • Wastewater • Continuous

harvesting • High exponential

growth • High lipid • CO2 from industries

gas

© MBG 2015 – All rights reserved 14

3. T

HE

ALG

AE

MA

RK

ET


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ton

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s d

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eig

ht

total production volume

1999

2004

2011

ChIR Symposium 2017

MICROALGAE BIOTECHNOLOGY INDUSTRIES HAS

GROWN FAST AND DIVERSIFIED:

• 75% IN HEALTH

FOOD MARKET AS DIETARY

SUPPLEMENT • 25% FEED AND

COSMETIC, FOOD ADDITIVES

• IT IS GROWING THE HIGH VALUE

COMPOUNDS MARKET

SIZE AND VALUE OF PRODUCTION


3. T

HE

ALG

AE

MA

RK

ET


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ton

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total production volume

1999

2004

2011

ChIR Symposium 2017

MICROALGAE BIOTECHNOLOGY INDUSTRIES HAS

GROWN FAST AND DIVERSIFIED:

• 75% IN HEALTH

FOOD MARKET AS DIETARY

SUPPLEMENT • 25% FEED AND

COSMETIC, FOOD ADDITIVES

• IT IS GROWING THE HIGH VALUE

COMPOUNDS MARKET

SIZE AND VALUE OF PRODUCTION


4. CULTIVATION: SITES & SYSTEMS


OPEN PONDS: OPEN PONDS – GREEN HOUSE: PHOTOBIOREACTORS:

PRODUCTIVITY PER SURFACE

COSTS

SPECIES VARIETY

QUALITY

ChIR Symposium 2017

Every species has its own peculiarities in terms of growth parameters, and for this reason, but not only, research and industry have develop several type of systems:

Open ponds present a great dependence

on weather conditions and high

risks of contamination, they

have low maintenance and

constructions costs


4. CULTIVATION: LOW PRODUCTION and LOW QUALITY

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References: Vardaka et al. 2016

«It is the best well known food supplement

commercially called Spirulina»

7,7

61,5

15,4

7,7 7,7 Europe

Asia

US

Central America

Oceania

ChIR Symposium 2017

An example of the importance of knowing microalgae can be found in the CYANOBACTERIA Arthrospira platensis:

Low globally production of 5000 tonnes per year despite of the high market demand

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© MBG 2015 - Tutti i diritti riservati

Spirulina OTU 42%

Other Cyanobacteri

a OTUs 58%

n = 31

6,4 %

6,4 % 3,2 %

19,4 %

References: Vardaka et al. 2016

ChIR Symposium 2017

No specific legislation for EU algae production First report on molecular diversity of commercially available «Spirulina» 18/31 : one to five additional cyanobacteria Due to environmental competition and contamination

While Spirulina is widely recognised with healthy aspects, there are really few studies on the quality and the risks associated with this algae.


Low quality of many Sprirulina based products due to several aspects:

Contamination

No research

No cleaning

No quality chain

No technology

Fungi Bacteria Patogens Other algae

(c) MBG 2017 - All right reserved References: Vardaka et al. 2016

ChIR Symposium 2017



5. A SOLUTION FOR AN ALL YEAR-LONG HQ PRODUCTION Combination of several aspects: high-tech greenhouse, monitoring and research


Innovative facilities and cultivation systems

Morphological and genetical analysis

Monitoring and chemical and physical

analysis

ChIR Symposium 2017


5. A SOLUTION FOR AN ALL YEAR-LONG HQ PRODUCTION Combination of high-tech greenhouse, monitoring and research


ChIR Symposium 2017

Analysis Frequency

visual check Every 3-4 days

growth Daily

pH - °C - lux Daily

Macro nutrients check up Every harvest

Nutritional values Every 1-2 weeks

High value compounds Every 2-3 weeks

Microbiological analysis Monthly

Patogens Every 2-3 months

Physico chemical analysis of water Every 6 months

Pesticides and heavy metals Every year

Toxins Every year

Performed by our Lab or external and certified Lab:

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Species specific systems of cultivations OPEN


5. CULTIVATION: SOLUTIONS

CLOSED

• ALGAE SPECIES • FIELDS OF APPLICATION • VOLUME OF PRODUCTION

• MATERIALS • RELATIONSHIP • SIZES

For each system:

ChIR Symposium 2017

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5. ALGAE CONTRIBUTION FOR THE HUMAN AND ENVIRONMENTAL BENEFITS

Production of High Quality microalgae healty

properties

i.e. Spirulina as microalga is a natural ″eater ″ of air

CO2 (O.8 kg of CO2 per kg of dry biomass)

Less water consumption


BEEF MEAT

50 L

WHEAT

6 L

SOY

3 L

SPIRULINA 1 L

WATER CONSUMPTION PER KG OF PROTEIN

ChIR Symposium 2017

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6. Missing…

NEW GREEN TECHNOLOGIES FOR INDUSTRIAL EXCTRACTION

• SAFE SOLVENTS • LOW ENERGY CONSUMPTION • RECYCLING METHODS • SCALABLE • COST SAVING • EFFICIENT

COMPOSITION OF LIPID EXCTRACTION FROM

P. tricornutum

TRADITIONAL NEW GREEN

fatty acids others

Data from a collaboration with University of Bologna – CIRI Lab Prof. Tagliavini

ChIR Symposium 2017

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5. Missing…

NEW GREEN TECHNOLOGIES FOR INDUSTRIAL EXCTRACTION

« world wholesale market for infant market US$ 10 billion per year»

Spolaore et al. 2006 DSM’s algae derived DHA is found in 99 % of all baby food in USA (Enzing et al. 2014)

ChIR Symposium 2017


Thank you!


ChIR Symposium 2017

Documents

ChIR Symposium 2017 Faro, 3-4 July - micoperibg.eu · ChIR Symposium 2017 Faro, 3-4 July Matilde Mazzotti (a), ... cyanobacteria Due to environmental competition and contamination