Chemical Composition Question

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    BIOLOGY MODULECHAPTER 4

    CHEMICAL COMPOSITION IN THE CELL

    WATER

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    NUCLEIC ACID

    - Store genetic information the form of a code-- basic units nucleotides

    P

    Q R

    Deoxyribonucleic acid (DNA) Ribonucleic acid (RNA)

    (4) polynucleotide strands

    twisted around each other in the form of a

    (5) . .

    Can be found in (6).. ,

    (7) ...and (8)...

    (9) . strand polynucleotide .

    Can be found in (10).,

    (11) ....and (12)....

    State P, Q and R :

    (1) P : .

    (2) Q : .

    (3) R : .

    Lubrication

    The importance

    ..

    Medium for biochemical

    High cohesion

    Moisture

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    LIPIDS

    - Consists of (13) . , (14) .. and (15) . .

    - Types of lipids- (16)., (17)., (18) ., (19).

    and steroids.

    - Fats and oils are (20).

    - Triglyceride is an (21).that is formed through the (22) . of one

    molecule of (23).and three molecules of (24) .

    Glycerol (25) Triglyceride (28)

    - waxes on the (29) . of the epidermis of leaves, fruits and seeds

    - (30) .. preventing the entry and evaporation of water

    - sebum-excreted from oil glands contains wax that soften the skin

    - Phospholipids-components in the formation of (31) .

    - Steroids-cholesterol

    - (32) . testosterone, oestrogen, progesterone

    - Fats and oils- consists of one molecule (33) .and three molecules of

    (34).

    - fatty acids- consists of long (35) . chain with a different number carbon

    atoms different fatty acids

    (26)

    (27)

    3 H2O

    Water

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    Fats

    Saturated fats Differences Unsaturated fats

    36 The presence of doublebonds between carbon

    atoms in fatty acids

    40

    37 Ability to react with anadditional hydrogen atom

    41

    38 Condition at roomtemperature

    42

    39Examples

    43

    CARBOHYDRATES

    Fill in the blanks.

    - Source of (44) .

    - Consists of . (45), . (46) and . (47)

    - Ratio of hydrogen atoms to oxygen atoms molecule is (48) .

    -

    Types of carbohydratesMonosaccharides Disaccharides Polysaccharides

    G

    Examples:(49) ...-in plants and fruits

    (50)

    -in sweet fruits and honey

    (51) ...- in milk- reducing sugar

    Glucose

    Glucose

    .. (52)- reducing sugar- malt sugar

    Glucose(53)

    (54) - (55)

    .- cane sugar

    -combination of hundreds of

    monosaccharides- formed by the condensation of

    (59)

    -do not taste sweet and do not

    crystallised

    - (60) ... in water

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    (79)

    Glucose

    56

    (57) ..- (58)

    ..- milk sugar

    Examples :

    (61) ....

    - in animals and yeast

    (62) .....

    - in plants

    (63) ..

    - cell walls of plants

    Process of formation and breakdown

    (64)(65)

    PROTEINS

    Fill in the blanks.

    - made up of the elements (66) ., (67) ., (68) . and

    (69)., (70) . and (71) .

    - made up of monomers called (72) .

    - A dipeptide consists of (73) .molecules of (74) . that are linked

    together by a (75) .. through (76) ..

    - Conversely, a (77) .can be broken down into by means of (78) ..

    Protein StructuresPrimary structures Secondary structures Tertiary structures

    - linear sequence of aminoacids in polypeptide chain

    -polypeptide coiled to forman (81)

    -helix(82)

    -the overall three-dimensionalshape of a polypeptide chain

    (85)

    Examples : enzymes, hormones,antibodies, plasma protein

    (80)

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    Quarternary structures-folded into (83) .sheets

    .. (84)

    - the combination of two or moretertiary polypeptides that makes

    up a protein(86)

    ENZYMES

    Fill in the blanks by using the words given.

    biological speed up catalyst

    - Enzymes are (92). .that (93) .biochemical reactionsin the cell.

    Types of amino acid(20 amino acid)

    (87) ..[9 amino acids]

    -cannot be synthesised by thebody- obtained from the diet

    - Why are animal proteins,first class proteins?(88)...

    non-essential amino acids[(89) . amino acids]

    -(90) synthesised by thebody- derived from other amino acids

    - Why are plant proteins,second class proteins?(91)...

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    The sites of enzyme synthesis

    Production of extracellular enzymes

    Intracellularenzymes

    General Characteristics ofEnzymes

    (96)

    (98)

    (100)

    (97)

    (99)

    Substrate Enzyme

    Lipid (101)(102) amylase(103) protease

    (94)

    (95)

    Definition (105)

    .

    Examples :(106)

    Extracellularenzymes

    Examples :(108)

    Definition (107)

    The sites of protein synthesis[ somiRboes](104)...

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    transported throughthe spaces

    proteins wrapped in. (117) thatbud off from the sides of theN:.(118)

    fuse with the membrane of the

    O :..(120).

    bud off from the. (121)membrane and

    travel to the. (122)membrane

    (113)K: .

    (112)(a)L:

    (109)O:

    (111)M: ...................

    (114)J: ...

    Proteins/ enzymes are synthesized inK : ..(115)

    (110)N: ..................................endoplasmic reticulum

    N : ...(116)

    L : .(119)

    Secretory vesicles

    Releasing the proteins outside the cell as[myzesne]. (123)

    (112)(b)L: ...

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    Lock and key mechanism

    - Choose the suitable substrate for the enzyme and complete the schematic diagrambelow to show the mechanism of enzyme action on a suitable substrate

    Enzyme Substrate

    (125) (126)

    Substrate

    Enzyme + Substrate Enzyme-Substrate Complex

    (127)

    Product

    Enzyme + Product

    caietv ietsRearrange the jumbled upwords to the correct terms:active site(124)

    -a distinctive shape thatcomplements its

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    temperature (oC)

    therateofenzymeactivity

    therateofenzym

    eactivity

    pH

    Lock and Key HypothesisConclusion :

    128

    129

    130

    FACTORS AFFECTING ENZYME ACTIVITY

    Factors Explanations Complete the diagram

    Temperature

    (132) temperature

    - reaction takes place slowly

    (133) temperature

    (37oC-40

    oC)

    - reaction at maximum rate

    (134) temperature

    (above 40oC)

    - enzymes are denatured

    - denaturation is irreversible

    (135)

    pH

    optimum pH

    - pH at which the rate of

    reaction is at the maximum

    - example :

    Pepsin (136) .

    Trypsin (137).

    Amylase (138)..

    - reversible

    (139)

    1 2 3 4 5 6 7 8 9 10

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    Substrate concentration

    therateofenzymea

    ctivity

    therateof

    enzyme

    activity

    Enzyme concentration

    Substrateconcentration

    - the rate of reaction is directly

    proportional to the substrate

    concentration until the reaction

    reaches a maximum rate

    - an increase in substrate

    concentration will speed up the

    reaction until the reaction reaches

    a (140)..

    - after the maximum rate the

    enzyme is said to be

    (141)..

    - limiting factors-

    (142)

    .

    (143)

    Enzymeconcentration

    - an increase in enzyme

    concentration will

    (144) ..the rate ofreaction until the reaction reaches

    a maximum rate

    - limiting factor-

    (145) .

    (146)

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    The uses of enzymes

    Match the enzyme to its uses

    Enzymes Uses

    In detergents

    (147)

    - tenderise meat- skinning of fish- used to remove hair from

    hides

    Lipase

    (148)

    - to convert starch to sugarin the making of syrup

    Amylase

    (149)

    - breaks down the cell wall

    of the seaweeds(extracting algae jelly fromseaweeds/

    - removal of seed coatsfrom cereal grain

    Proteases

    (150)

    - is used in the ripening ofcheese

    - to produce flavour incheese

    - to break up fats in meatCellulases

    (151)- effectively dissolve

    proteins stains

    The Importance of Chemical Composition in Cells

    - Complete the table below

    Chemicalcomponents

    Function Consequence of defiency

    Water(152) (157)

    Lipids

    (153) (158)

    Carbohydrates(154) (159)

    Proteins(155) (160)

    Enzymes(156) (161)

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    STRUCTURAL QUESTIONS:

    1 (a) Enzymes is widely used in our daily life and industries.

    Explain how enzymes act in :(i) helping to cook meat.

    ...

    ...

    ...

    [2 marks]

    (ii) extracting agar from seaweeds....

    ...

    ...

    [3 marks]

    2 Figure 1(a) shows a reaction catalysed by lipase.

    Figure 1(a)

    (a) (i) Label P,Q, R and S in Figure 1(a). [4 marks]

    (ii) State two characteristics of P.

    ...

    ...

    ...

    [2 marks]

    Q:

    P:

    R: S:

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    (b) (i) State the principle of enzymic reaction as shown in Figure 1(a)....

    [1 mark]

    (ii) What can you deduce about the enzyme lipase based on Figure1(a)?...

    ...

    [1 mark]

    (c) Enzymes are used widely in industries.State the use of lipase in food industry.....

    [1 mark]

    (d) The temperature at which a lipase reaction takes place is increased tomore than 60OC.State the effect on the quantity of the product. Explain your answer.

    ....

    ....

    ..

    [2 marks]

    (e) Figure 1(b) shows a box of washing powder.

    Figure 1(b)

    A housewife uses cold water to wash her clothes with brand X washingpowder. The cleaning is less effective.Using the above information, explain why.

    ....

    ....

    ....

    [2 marks]

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    3 (a) Diagram 2.1 shows a mechanism of an enzyme reaction.Draw the diagram in the spaces III and IV provided in Diagram 2.1to complete the mechanism of the enzyme reaction.

    Diagram 2.1

    (b) In a food preparation process, a housewife carries out thefollowing steps:Step 1 : Marinate pieces of meat with strips of unripe papaya in a

    basin.

    Step 2: Immerse the basin in a container filled with water at 40oCfor 30 minutes as Diagram 2.2 .

    Diagram 2.2

    Explain why the housewife carries out Step 1 and Step 2....

    ...

    ...

    ...

    ...

    ...

    [4 marks]

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    ESSAY QUESTIONS

    4. Diagram shows the effect of a rise in temperature on the reaction rate of an enzyme.

    (a) Based on the above graph:

    (i) Explain why a rise in temperature increases the rate of reaction until it reaches an

    optimum.

    [ 5 marks]

    (ii) Explain what causes the decrease in the rate of reaction when the temperature

    exceeds the optimum temperature.

    [ 5 marks]

    (b) Enzymes are widely use in our homes and industries. Discuss how the enzymes areused in industries.

    [10 marks ]

    Enzymes are widely used in our daily life and industries.

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