CHEMISTRY    

Paper  16  Bioorganic  &  Biophysical  Chemistry  Module  33  Electrophoresis  and  Isotopes  in  Biology.  

 

KNOW MORE

Web-links

• http://en.wikipedia.org/wiki/Gel_electrophoresis • arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/principles.html • http://www.youtube.com/watch?v=pnBZeL8nFEo • http://www.ausetute.com.au/nuclesum.html • http://www.launc.tased.edu.au/online/sciences/PhysSci/radres/isotMe.htm

Suggested Readings

• Principles and Techniques of Biochemistry and Molecular Biology. 7th Edition,

Edited by Keith Wilson and John Walker

 

CHEMISTRY    

Paper  16  Bioorganic  &  Biophysical  Chemistry  Module  33  Electrophoresis  and  Isotopes  in  Biology.  

 

• Modern Experimental Biochemistry. Third Edition, By Rodney F. Boyer

 

CHEMISTRY    

Paper  16  Bioorganic  &  Biophysical  Chemistry  Module  33  Electrophoresis  and  Isotopes  in  Biology.  

 

Introductory Practical Biochemistry. By S. K. Sawhney and Randhir Singh

• Analytical Chemistry. Sixth Edition, By Gary D. Christian

• Radioisotopes in Biology. Second Edition, Edited By Robert J. Slater

 

CHEMISTRY    

Paper  16  Bioorganic  &  Biophysical  Chemistry  Module  33  Electrophoresis  and  Isotopes  in  Biology.  

 

Glossary

A • Alpha Particle: It is composed of two neutron plus two protons, the same as the

helium nucleus. B

• Beta Particles: A beta particle (also written as b-particle) is the same as an electron.

C • Chromatography: The separation of components of a mixture (the mobile phase) by

passing it through another phase (the stationary phase), making use of the different extents to which the various components are adsorbed by the stationary phase.

D

• Denaturation: Structural change in macromolecules caused by extreme conditions • DNA Footprinting: Technique used to determine the binding of proteins to specific

regions of DNA.

F • Free radical : A molecule with an unpaired electron

G

• Gamma Rays: Electromagnetic radiation of very short wavelength, less than 3Î10-11 m, highly penetrating.

• Gel: A gel is a colloid in a solid form

M • Mutation: An abrupt change in the genetic characteristics of an organism.

P • Photochemical Polymerization: Polymerization initiated by the absorption of energy

in the form of light.

R • Radioactive Tracer: A radioactive isotope of an element substituted specifically in a

compound in order to tag it.

X • X-rays: invisible electromagnetic radiation having a much shorter wavelength than

light, between 0.01 – 10 nm. They are produced by the transitions of electrons in the inner level of excited atoms or by the rapid deceleration of charged particles.

 

CHEMISTRY    

Paper  16  Bioorganic  &  Biophysical  Chemistry  Module  33  Electrophoresis  and  Isotopes  in  Biology.  

 

Time-Lines

Timelines Image Description

1895

Wilhelm Rontgen, German Physicist. Discovered X-ray. Received noble prize in Physics in 1901. In 2004, the International Union of Pure and Applied Chemistry (IUPAC) named element 111, roentgenium, a radioactive element with multiple unstable isotopes, after him.

1896

Antoine Henri Becquerel discovered radioactivity. Becquerel is SI unit of radioactivity, named after him. Henri Becquerel shared Nobel Prize with Pierre and Marie Curie in 1903 for their work in discovering radioactivity.

1903

Marie Curie won the Nobel Prize with Becquerel and Pierre Curie for the discovery of natural radioactivity.

 

CHEMISTRY    

Paper  16  Bioorganic  &  Biophysical  Chemistry  Module  33  Electrophoresis  and  Isotopes  in  Biology.  

 

1913

Frederick Soddy proved the existence of isotopes of certain radioactive elements. Got Nobel Prize for chemistry in 1921.

1935

Irene-Joliot Curie, discovered artificial radioactivity and awarded Nobel Prize in Chemistry with her husband Frederic Joliot –curie.