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AP1 Chapter 3 1
Chapter 3:Cell Biology
AP1 Chapter 3 2
I. Fxns of a cell• Cell
– the basic unit of all living things (the smallest part of a living organism in our case humans)
• Shared Characteristics– Plasma Membrane
• Outer boundary of the cell though which the cell interacts with its external environment
– Nucleus• Directs activities of the cell
– Cytoplasm• Most cell activities occur here
– Organelles• Perform specific fxns in cell
I. Fxns of a cell 4 major fxns of cellsA. Cell metabolism & e+ use:
– Metabolic rxns in the cell e+ is released to accomplish cell activities & maintain Body Temp.
B. Synthesis of Molecules– Can prod proteins, nucleic acids, lipids– Cell’s fxn & characteristics are
determined by the molecules they prod
C. Communication– Cells prod & respond to chemical & e+
signals wh/ allows them to communicate w/each other
– * Neuron to a muscle = contraction or relaxation
D. Reproduction & inheritance– Cell holds the genome. It determines
structure & fxnl characteristics of that cell.
– Cells can prod new cells – Gametes transmit genetic info. 3AP1 Chapter 3
AP1 Chapter 3 4
III. Plasma Membrane
AP1 Chapter 3 5
III. Plasma Membrane• Outer most component of the cell• Fxns as a boundary to separate
inside from outside of the cell (intra vs extra cellular)
• Encloses and supports the cell’s contents
• Attaches the cell to the extracellular environment or to other cells
• Cell’s ability to recognize & communicate with each other occurs via the plasma membrane
• Determines what moves into ot out of the cell (therefore the contents inside the cell can be different from what is outside).
• Membrane potential: electrical charge difference across the PM resulting from the cell’s regulation of movement into and out of the cell
Make-up:• 45-50% Lipids• 45-50% Proteins• 4-8% Carbohydrates
6
III. P
lasm
a M
embr
ane
• Terminology– Intracellular: (Intra- Inside) inside the cell– Extracellular: (Extra- Outside) outside of the cell– Intercellular: (Inter- Between) from 1 cell to another– Glycoproteins: carb’s + proteins– Glycolipids: Carb’s + Lipids– Membrane Potential: result of uneven distribution of ions on the
inside verses the outside of the cell
• Glycocalyx:– Collection of glycoproteins, glycolipids, and carb’s that lies on the
outer surface of the plasma membrane
AP1 Chapter 3 7
IV. Membrane Lipids
AP1 Chapter 3 8
IV. M
embr
ane
Lipi
ds• Phospholipids (PL’s):
– Predominant lipid in membrane
– Bipolar:• Hydrophilic “head” is water
loving and polar and faces both surfaces (inner & outer)
• Hydrophobic “tail” is water hating and non-polar and crowd together between the heads
• Cholesterol– Makes up 1/3 of the
membrane and lie btwn PL’s– Helps to limit PL’s mvmt
providing stability for the PM– It is also bipolar and the “tail”
is embedded in head while the ring is embedded in the tails
AP1 Chapter 3 9
IV. Membrane Lipids
• *Fluid Mosaic Model*– The lipid bilayer is mobile with things floating
w/in it– Consequences of this:
a) Important for molecule distribution in the membrane
b) Slight damage can be repaired because the PL’s will move to cover it
c) It enables two different membranes to fuse with each other
AP1 Chapter 3 10
V. Membrane ProteinsA. Marker Molecules
B. Attachment Proteins
C. Transport Proteins
D. Receptor Proteins
E. Enzymes
AP1 Chapter 3 11
V. Membrane Proteins (MP)
• Many fxns of the plasma membrane are determined by the combination of membrane proteins present.
• The ability of these proteins to fxn properly is determined by their 3-D shape
• There are 2 major types:1. Integral/Intrinsic
• (Transmembrane) contain hydrophobic and hydrophilic regions to match the phospholipids characteristics & location
2. Peripheral/Extrinsic• Surface proteins on inner or outer surface.• Can be bound to an integral protein or the phospholipids head
• There are 5 major classes of MP’s
AP1 Chapter 3 12
5 major classes of Membrane proteins
I. Markermolecules
II. AttachmentProteins
III. TransportProteins
IV. ReceptorProteins
V. Enzymes
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AP1 Chapter 3 13
Membrane Proteins: I. Marker Molecules
• Glycoproteins or glycolipids that allow for cells to identify other cells or other molecules
• Important because cells aren’t isolated and must function as a whole for normal body function.
• May be integral or peripheral proteins
• Ex/ immune cells
AP1 Chapter 3 14
Membrane Proteins: II. Attachment Proteins
• Integral proteins that may attach to intracellular molecules.
• Integrins can also function in cellular communication.
AP1 Chapter 3
Membrane Proteins: III. Transport Proteins
All exhibit 3 characteristics
1. Specificity– Each binds to & transports
only 1 types of molecule/ion
2. Competition– Closely related substances
may bind to the same binding site & the one w/ greater [ ] or higher affinity is more readily moved across the PM
3. Saturation– Movement is limited by the #
of transport proteins rate will eventually plateau because the # of proteins are going at their maximum rate
15
Membrane Proteins: III. Transport Proteins
A. Channel Proteins• Form passageways through the
plasma membranes that have both hydrophobic and hydrophilic regions.
B. Carrier Proteins• Move larger ions or molecules
across the membrane, when bound it changes shape to allow it to move from one side of the membrane to the other then return to its original shape to work again.
C. ATP-powered pumps• Moves ions or molecules across the
membrane using ATP.
AP1 Chapter 3 16
Membrane Proteins: III. Transport Proteins
A. Channel Proteinsa. Non-gated Channels
• Always open responsible for the permeability of the plasma membrane when the cell membrane at rest.
b. Gated Channels• Can be opened or closed
i. Ligand Gated – Small molecules must bind in
order to open or close the channel
ii. Voltage Gated– Change in voltage across the
plasma membrane causes the gate to open
AP1 Chapter 3 17
Membrane Proteins: III. Transport Proteins
B. Carrier Proteinsa. Uniporter
• Movement of 1 ion or molecule across the plasma membrane.
b. Symporter• Movement of 2 ions or molecules in
the same direction (into the cell or out of the cell).
c. Antiporter• Movement of 2 ions or molecules in
opposite directions (one in and one out or vise versa).
AP1 Chapter 3 18
Membrane Proteins: III. Transport Proteins
C. Sodium-Potassium Pump• These have 2 binding sites.
One is for the molecule to be moved the other is for ATP
• Breakdown of ATP releases e+ Ding shape of the “pump” protein which moves the molecule across the membrane
AP1 Chapter 3 19
AP1 Chapter 3 20
Membrane Proteins: IV. Receptor Proteins
• Proteins or glycoproteins in the plasma membrane that have an exposed receptor site on the outer cell surface which can attach to specific chemical signals.
• Many are part of an intercellular communication system that coordinates cell activities.
AP1 Chapter 3
Membrane Proteins: IV. Receptor Proteins
A. Receptor linked to channel proteins
– These help form ligand-gated channels & when bound it changes the channels shape to move ions
B. Receptors linked to G-protein complexes
– Uses a second messenger system, binding of the receptor externally causes to the cell internally
• 3 ways a can stimulate a cellular response
1. Intracellular chemical signals
2. Opening channels in the plasma membrane
3. Activation of enzymes associated with the plasma membrane
21
AP1 Chapter 3 22
Membrane Proteins: V. Enzymes
• These may work on the inner or outer surface of the plasma membrane.
• Some are always active but others are activated by things like GPCR’s
AP1 Chapter 3 23
VI. Movement through the plasma membrane
AP1 Chapter 3 24
V. Mvmt thru the PM• Inside of the cell:
– Enzymes other proteins, glycogen, high potassium concentration
• Outside of the cell:– High concentration of sodium, calcium, & chloride
• The cell has to be able to bring in nutrients inside and get waste products out without changing the cell’s volume, because too much can cause the cell the rupture (causing cell death) or to shrivel (also causing cell death).
• Movement1. Molecules that are lipid soluble or very small water soluble molecules
will freely go across the plasma membrane.
2. Large lipid soluble molecules and water soluble molecules can’t pass through the plasma membrane and may need to use transport proteins.
3. Larger water soluble molecules or whole cells may be moved by vesicles.
AP1 Chapter 3 25
Membrane transport mechanisms
A. Passive Transport Mechanisms• No energy required to move
molecules from one side of the membrane to another
B. Active Transport Mechanisms• Energy required to move
molecules from one side of the membrane to another
AP1 Chapter 3 26
Membrane transport mechanisms
Passive Transport Mechanisms
A. Diffusion
B. Osmosis
C. Facilitated Diffusion
Active Transport Mechanisms
A. Active Transport
B. Secondary Active Transport
C. Vesicular Transporta. Endocytosis
b. Exocytosis
27
Passive Transport MechanismsA. Diffusion:
– Movement of solutes from an area of high concentration to an area of low concentration
– Concentration gradient: concentration difference between 2 points divided by the distance between the 2 points,
– Rate of Diffusion1. Magnitude of the concentration gradient
2. Temperature of the solution
3. Size of the diffusion molecules
4. Viscosity of the solvent
AP1 Chapter 3
AP1 Chapter 3 28
Passive Transport MechanismsB. Osmosis
– Diffusion of water across a selectively permeable membrane
– Will allow water but not all solutes with in the water
– Important because it can influence a cell’s function when water moves.
– Osmotic Pressure:• Force required to prevent water
movement across a selectively permeable barrier via osmosis
1) Isosmotic
2) Hyperosmotic
3) Hyposmotic
Concentration of solutions
AP1 Chapter 3 29
Passive Transport MechanismsB. Osmosis
• Tonicity: refers to the cell’s shape remaining constant b/c it maintains it’s internal pressure
1. Isotonic sol’n: no net mvmt of H2O, cell doesn’t D shape
2. Hypertonic sol’n: mvmt out of cell b/c sol’n has a greater [ ] of solute thus a higher osmotic pressure (crenation)
3. Hypotonic sol’n: mvmt into cell b/c sol’n has a lower [ ] of solute thus a lower osmotic pressure (Lysis)
30
Passive Transport MechanismsC. Facilitated Diffusion
– Amino acids & glucose go into the cell and area going out of the cell can’t occur via direct diffusion because they are too big. Thus there is
– Mediated transport (facilitated diffusion):• Process by which transport proteins assist the movement of
water soluble molecules or electrically charged molecules or ions across the plasma membrane.
AP1 Chapter 3
Active Transport Mechanisms
A. Active Transport• also a type of mediated
transport. Requires energy provided by ATP movement dependent on the number of pumps and availability of ATP.
• Important because it can move things against their concentration gradients.
AP1 Chapter 3 31
Active Transport Mechanisms
B. Secondary Active Transport
• Passive transport of 1 molecule with its concentration gradient helps to energize the carrier so that it can transport the second molecule against its concentration gradient.
AP1 Chapter 3 32
Active Transport Mechanisms
C. Vesicular Transport• Movement of larger
volumes of substances across the plasma membrane through the formation and release of vesicles requiring ATP.
• BUT…the specificity seen in others doesn’t occur in this process. a. Endocytosis
i. Pinocytosis
ii. Phagocytosis
iii. Receptor mediated endocytosis
b. ExocytosisAP1 Chapter 3 33
AP1 Chapter 3 34
Active transport mechanismsa) Endocytosis: uptake
of material into the cell.
• 3 types:i. Pinocytosis
• Molecules dissolved in liquid
ii. Phagocytosis• Cells and solid
particles
iii. Receptor mediated endocytosis
• Specificity for substances
Pin
ocyt
osis
Pha
gocy
tosi
s
Rec
epto
r-m
edia
ted
Active Transport Mechanisms
b. Exocytosis– Materials
manufactured by the cell are packaged in secretory vesicles that fuse w/the PM & release their contents outside of the cell
– ATP req’d– Proteins & other water
soluble substances
AP1 Chapter 3 35
AP1 Chapter 3 36
VII. Cytoplasm
The material outside the nucleus and inside of the plasma membrane
http://www.animalcute.net/wp-content/uploads/2012/01/Animal-Cell-Cytoplasm5.jpg
AP1 Chapter 3 37
VII. Cytoplasm
1. Fluid Portion• Anatomy:
– H2O w/dissolved ions & molecules; colloid w/many suspended molecules espicially proteins
• Physiology:– Contains enz’s
that catalyze decomposition & synthesis rxns, ATP is also prod’d in glycolysis rxns
3. Cytoplasmic Inclusions
• Anatomy: – Collections of
molecules manufactured or ingested by cell and may be membrane bound
• Physiology– Fxn is dependent
on the molecules– Ex: energy
storage lipids&glycogen
(1/2 cytosol/1/2 organelles)
3 parts to cytoplasm
2. Cytoskeleton• Supports cell &
holds nucleus & organelles in place
• Responsible for mvmt in & of the cell
• Made up of 3 groups of proteins:
a) Microtubules
b) Microfilaments
c) Intermediate filaments
38
VII. Cytoplasm• 2. Cytoskeleton
• Made up of 3 groups of proteins:
a) Microtubules• Provide support & structure to
cytoplasm• Involved in cell division &
transport of intracellular materials
• Form essential components of organelles (centrioles, spindle fibers, cilia, & flagellum)
b) Actin Filaments• Provide structure to cytoplasm &
mechanical support for microvilli• Responsible for cell mvmts
c) Intermediate filaments• Protein Fibers• Provide strength to cells
AP1 Chapter 3 39
VIII. The Nucleus & Cytoplasmic Organelles
A. Nucleus
B. Cytoplasmic Organelles:
A. Ribosomes
B. Endoplasmic Reticulum
C. Golgi Apparatus
D. Secretory Vesicles
E. Lysosome
G. Peroxisomes
H. Proteosomes
I. Mitochondria
J. Centrioles & Spindle Fibers
K. Cilia & Flagella
L. Microvilli
• Organelles: structures w/in cells that are specialized for a particular fxn.• Number & types within each cell are related to the specific structure &
function of the cell• Largest organelle Nucleus
• (all others are considered cytoplasmic organelles)
AP1 Chapter 3 40
VIII. The Nucleus & Cytoplasmic OrganellesA. Nucleus
• Large membrane bound structure usually centrally located – Shape & # of lobules vary dependent on cell
type (may be multiple or spit out of cell)– 3 Major structures of the nucleus:
41
IX. T
he Nucleus &
Cytoplasm
ic Organelles
A. N
ucleus• 3 Major structures of the
nucleus
1. Nuclear Envelope– Double membrane enclosing the
nucleus that separates the nucleus from the cytoplasm, also uses nuclear pores to regulate mvmt in/out of the nucleus
2. Chromatin– Thin strands of DNA wound
around histones (proteins) that regulate protein synthesis thus also regulating the chemical rxns in the cell
3. Nucleolus– 1 or more dense bodies
consisting of ribosomal RNA , 10 stretches of DNA called nuclear organizer regions that contain the rRNA templates, & proteins that serve as the assembly site for ribosomal subunits
AP1 Chapter 3
IX. The Nucleus & Cytoplasmic OrganellesB. Ribosomes
• rRNA & proteins make-up both the large & small subunits of ribosomes
• Site of protein synthesis• 2 categories:
1. Free: mainly prod proteins that will be used inside the cell
2. Bound: mainly prod proteins that will be excreted from the cell
C. Endoplasmic Reticulum• Series of membranes
continuous w/ the nuclear envelope distributed throughout the cell
• 2 major types:– Rough ER: ribosomes attached
fxns in protein synthesis & transport of those to the golgi
– Smooth: no ribosomes fxns in manufacture of lipids & carb’s; detoxifies harmful chemicals; can store Ca2+
42
IX. The Nucleus & Cytoplasmic Organelles
E. Golgi Apparatus (GA)
• membrane bound sack from the ER that goes to the GA for processing
F. Secretory Vesicle• Membrane bound sac that
pinches off of the GA that carries the finalized proteins & lipids to the cell surface for secretion
• These can accumulate in the cell until proper signal is received to initiate its release
AP1 Chapter 3 43
D. Transport Vesicle
• Flattened membrane sacs stacked on each other modifies, packages, & distributes proteins & lipids prod’d by RER & SER for secretion or internal use
• Can concentrate, or chemically modifies by adding carbs or lipids making glycoproteins or glycolipids
AP1 Chapter 3 44
IX. The Nucleus & Cytoplasmic Organelles
F. Lysosome
• Membrane bound sac pinched off of GA carries digestive enz’s that can break down NA, polysaccharides, lipids, thus old cell parts & phagocytized bacteria
G. Peroxisome
• Membrane bound vesicle 1 site of fatty acid & AA degradation byproduct is H2O2 using catalase brksdwn to H2O + O2
• High # in liver & kidney cells
AP1 Chapter 3 45
IX. The Nucleus & Cytoplasmic OrganellesH. Proteasome
• Tube-like protein in the cytoplasm as proteins enter the cell they are enzymatically degraded in the cytoplasm
I. Mitochondria
• Dynamic structure enclosed by a double membrane. Inner projections are called cristae. Major site of ATP-synthesis when O2 is available.
• #/cell based on cell fxn
AP1 Chapter 3 46
IX. The Nucleus & Cytoplasmic Organelles
J. Centrioles• Pair of cylindrical organelles
in centrosome made-up of microtubules
• Center for microtubule formation
• Determine polarity of the cell during division
• For basal bodies of cilia & flagellum
K. Cilia• Short hair-like Extensions
of the PM using microtubules to hold their shape
• Fxnally in humans it moves materials over cell surface
• Basal Body: located at base of cilia
AP1 Chapter 3 47
IX. The Nucleus & Cytoplasmic OrganellesL. Flagellum
• Long Hair-like extensions of the PM using microtubules to hold their shape.
• Responsible for the mvmt of sperm
M. Microvilli
• Short extension of the PM containing microfilaments
• Increase surface area of the PM for absorption or secretion.
• May fxn as a modified sensory receptor (hair cells in ear)