Chapter 17

DNA Testing in High-Profile Cases

©2002 Academic Press

Historical Investigations

• Romanovs– Tsar Nicholas II– Anna Anderson (not Anastasia)

• Tomb of the Unknown Soldier– mtDNA used to match bones with family

• Thomas Jefferson– Y chromosome: 12 STRs and 7 SNPs– match with Sally Hemming’s descendants

©2002 Academic Press

Lineage of Romanov Family with mtDNA Results

TsarinaAlexandra

Tsar Nicholas II

Xenia Cheremeteff-Sfiri

Xenia Cheremeteff-Sfiri

Prince PhilipDuke of Edinburgh

Prince PhilipDuke of Edinburgh

GeorgijRomanov

GeorgijRomanov

Mitotype16111T16357C263G

315.1C

Mitotype16126C16169T16294T16296T

73G263G

315.1C

16169T/C16169T/C

Louise of Hesse-Cassel

Louise of Hesse-Cassel

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Tomb of Unknown SoldierIdentification of Skeletal Identification of Skeletal Remains from Previous Remains from Previous Military ConflictsMilitary Conflicts

Identification ofIdentification ofMichael J. Blassie - Vietnam Michael J. Blassie - Vietnam Tomb of the UnknownTomb of the Unknown

>2100 Unaccounted for >2100 Unaccounted for from SEAfrom SEA

>8000 Unaccounted for >8000 Unaccounted for from Koreafrom Korea

>75,000 Unaccounted for >75,000 Unaccounted for from WWIIfrom WWII

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Thomas Woodson

Different Y Haplotype

Thomas Jefferson II

Field Jefferson Peter Jefferson

President Thomas Jefferson

Eston Hemings

Same Y Haplotype

Jefferson Y Haplotype

Jefferson Y Haplotype

?

Nature article (Nov 1998)

Jefferson-Hemings Study

©2002 Academic Press

DNA MarkerTested

FieldJefferson

Male-Line

EstonHemings

Male-Line

John CarrMale-Line

Thomas WoodsonMale-Line

# individuals typed Five One Three Five

Y STR LociDYS19 15 15 14 14

DYS388 12 12 12 12DYS389A 4 4 5 5DYS389B 11 11 12 11DYS389C 3 3 3 3DYS389D 9 9 10 10DYS390 11 11 11 11DYS391 10 10 10 13DYS392 15 15 13 13DYS393 13 13 13 13

DXYS156Y 7 7 7 7

Y SNP Loci (0=ancestral state; 1=derived state)DYS287 (YAP) 0 0 0 0

SRYm8299 0 0 0 0DYS271 (SY81) 0 0 0 0

LLY22g 0 0 0 0Tat 0 0 0 0

92R7 0 0 1 1SRYm1532 1 1 1 1

Minisatellite Locus

MSY1(3)— 5(1)—14(3)—32(4)—16

(3)— 5(1)—14(3)—32(4)—16

(1)—17(3)—36(4)—21

(1)—16(3)—27(4)—21

Results from Jefferson-Hemings Study

©2002 Academic Press