View
221
Download
1
Tags:
Embed Size (px)
Citation preview
Topics to discuss
• DNA binding proteins
• Prokaryotic gene regulation– negative inducible: lac operon– negative repressible: trp operon
• Eukaryotic gene regulation
Now let’s look at 2 (of four) groups of operons
Negative inducible operons
Negative repressible operons
Now let’s look at 2 specific operons
• The Lactose operon: negative inducible
• The Tryptophan operon: negative repressible
Francois Jacob and Jacques Monod 1961
• Studied lactose metabolism/operon in prokayotes
J. Monod
Won the Nobel Prize in 1965
Z Y AOPI
Lac Operon and Proposed Arrangement with control I gene
Not technically part of the “operon”
Next: let’s review just the pattern of expression when:
• When only lactose is present.
• When only glucose is present.
• Glucose and lactose are present in the cell
To answer this question you need to understand this reaction
Adenylyl cyclase Cyclic AMPATP
Glucose may inhibit this enzyme
catabolite activator protein
+
Changes in Chromatin structure and eukaryotic gene regulation
• Histone acetylation
• DNA methylation
• RNA silencing
Do eukaryotes show coordinate gene regulation?
Yes, the same response element may be found in related genes.