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Certificate of Analysis: Lyophilized Microorganism Specification and Performance Upon Release Specifications Microorganism Name: Enterococcus faecalis Catalog Number: 0366 Lot Number: 366-157 Reference Number: ATCC® 29212™* Purity: < 0.1% Total Pellet CFU Recovery: > 1000 CFUs per Pellet Passage from Reference: 4 Expiration Date: 2015/02 Release Information: Quality Control Technologist: Megan Murn Release Date: 2013/4/17 © 2012 Microbiologics, Inc. All Rights Reserved. 200 Cooper Avenue North Saint Cloud, MN 56303 Page 1 of 2 DOC.286 Performance Macroscopic Features: Medium: Small to medium, gray/white, translucent, smooth, circular with entire edge SBAP Microscopic Features: Method: Gram positive ovoid cells, mostly in pairs or short chains Gram Stain (1) Vitek GP (1) Phenotypic Features Results D-AMYGDALIN + PHOSPHATIDYLINOSITOL PHOSPHOLIPASE C - D-XYLOSE - ARGININE DIHYDROLASE 1 + BETA-GALACTOSIDASE - ALPHA-GLUCOSIDASE + Ala-Phe-Pro-ARYLAMIDASE - CYCLODEXTRIN - L-Aspartate ARYLAMIDASE + BETA GALACTOPYRANOSIDASE - ALPHA-MANNOSIDASE - PHOSPHATASE - Leucine ARYLAMIDASE + L-Proline ARYLAMIDASE - BETA GLUCURONIDASE - ALPHA-GALACTOSIDASE - L-Pyrrolydonyl-ARYLAMIDASE + BETA-GLUCORONIDASE - Alanine ARYLAMIDASE + Tyrosine ARYLAMIDASE + D-SORBITOL + UREASE - POLYMIXIN B RESISTANCE + D-GALACTOSE + D-RIBOSE + L-LACTATE alkalinization - LACTOSE - N-ACETYL-D-GLUCOSAMINE + D-MALTOSE + BACITRACIN RESISTANCE + NOVOBIOCIN RESISTANCE + GROWTH IN 6.5% NaCl + D-MANNITOL + D-MANNOSE + METHYL-B-D-GLUCOPYRANOSIDE + PULLULAN - D-RAFFINOSE - O/129 RESISTANCE (comp.vibrio.) + SALICIN + SACCHAROSE/SUCROSE + D-TREHALOSE + ARGININE DIHYDROLASE 2 + OPTOCHIN RESISTANCE + Other Features/ Challenges: Results (1) Catalase (3% Hydrogen Peroxide): negative (1) Bile Esculin Agar: positive (1) Streptomycin (300 mcg - Disk Susceptibility): 14 - 20 mm (1) Gentamicin (120 mcg - Disk Susceptibility): 16 - 23 mm (1) SXT (1.25/23.75 - Disk Susceptibility): >/= 20 mm BHIA w/Vancomycin (6 mcg/ml): Sensitive Brad Goskowicz, President AUTHORIZED SIGNATURE

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  • Certificate of Analysis: Lyophilized Microorganism Specification and Performance Upon ReleaseSpecificationsMicroorganism Name: Enterococcus faecalisCatalog Number: 0366Lot Number: 366-157Reference Number: ATCC 29212*Purity: < 0.1% Total Pellet CFURecovery: > 1000 CFUs per PelletPassage from Reference: 4

    Expiration Date: 2015/02Release Information:Quality Control Technologist: Megan MurnRelease Date: 2013/4/17

    2012 Microbiologics, Inc. All Rights Reserved. 200 Cooper Avenue North Saint Cloud, MN 56303 Page 1 of 2 DOC.286

    PerformanceMacroscopic Features: Medium:Small to medium, gray/white, translucent, smooth, circular with entire edge SBAPMicroscopic Features: Method:Gram positive ovoid cells, mostly in pairs or short chains Gram Stain (1)Vitek GP (1)Phenotypic Features ResultsD-AMYGDALIN +PHOSPHATIDYLINOSITOL PHOSPHOLIPASE C -D-XYLOSE -ARGININE DIHYDROLASE 1 +BETA-GALACTOSIDASE -ALPHA-GLUCOSIDASE +Ala-Phe-Pro-ARYLAMIDASE -CYCLODEXTRIN -L-Aspartate ARYLAMIDASE +BETA GALACTOPYRANOSIDASE -ALPHA-MANNOSIDASE -PHOSPHATASE -Leucine ARYLAMIDASE +L-Proline ARYLAMIDASE -BETA GLUCURONIDASE -ALPHA-GALACTOSIDASE -L-Pyrrolydonyl-ARYLAMIDASE +BETA-GLUCORONIDASE -Alanine ARYLAMIDASE +Tyrosine ARYLAMIDASE +D-SORBITOL +UREASE -POLYMIXIN B RESISTANCE +D-GALACTOSE +D-RIBOSE +L-LACTATE alkalinization -LACTOSE -N-ACETYL-D-GLUCOSAMINE +D-MALTOSE +BACITRACIN RESISTANCE +NOVOBIOCIN RESISTANCE +GROWTH IN 6.5% NaCl +D-MANNITOL +D-MANNOSE +METHYL-B-D-GLUCOPYRANOSIDE +PULLULAN -D-RAFFINOSE -O/129 RESISTANCE (comp.vibrio.) +SALICIN +SACCHAROSE/SUCROSE +D-TREHALOSE +ARGININE DIHYDROLASE 2 +OPTOCHIN RESISTANCE +

    Other Features/ Challenges: Results(1) Catalase (3% Hydrogen Peroxide): negative(1) Bile Esculin Agar: positive(1) Streptomycin (300 mcg - Disk Susceptibility): 14 - 20 mm(1) Gentamicin (120 mcg - Disk Susceptibility): 16 - 23 mm(1) SXT (1.25/23.75 - Disk Susceptibility): >/= 20 mmBHIA w/Vancomycin (6 mcg/ml): Sensitive

    Brad Goskowicz, PresidentAUTHORIZED SIGNATURE

  • Certificate of Analysis: Lyophilized Microorganism Specification and Performance Upon ReleaseSpecificationsMicroorganism Name: Enterococcus faecalisCatalog Number: 0366Lot Number: 366-157Reference Number: ATCC 29212*Purity: < 0.1% Total Pellet CFURecovery: > 1000 CFUs per PelletPassage from Reference: 4

    Expiration Date: 2015/02Release Information:Quality Control Technologist: Megan MurnRelease Date: 2013/4/17

    2012 Microbiologics, Inc. All Rights Reserved. 200 Cooper Avenue North Saint Cloud, MN 56303 Page 2 of 2 DOC.286

    Disclaimer: The last digit(s) of the lot number appearing on the packing slip is merely a packaging event number. The lot number displayed on this certificate is the actual base lot number.

    Note for Vitek: Although the Vitek panel uses many conventional tests, the unique environment of the card, combined with the short incubation period, may produce results that differfrom published results obtained by other methods.

    Refer to the enclosed product insert for instructions, intended use and hazard/safety information.

    Individual products are traceable to a recognized culture collection.

    (*) The ATCC Licensed Derivative Emblem, the ATCC Licensed Derivative word mark and the ATCC catalog marks are trademarks of ATCC.Microbiologics, Inc. is licensed to use these trademarks and to sell products derived from ATCC cultures.

    (1) These tests are accredited to ISO/IEC 17025:2005.