Supplementary Figure 3. Examples of embryos in which Cer-1/GFP positive cells lose expression (A, white arrow) or divide(B, white arrow) during culture from E4.75. Note that only one of the z-planes is shown. The times on each panel indicate the time after the time-lapse had been started. The corresponding schematic diagram for each of the embryos is shown for each panel and the tissues are indicated. Epi, epiblast, VE, visceral endoderm; ExE, extraembryonic ectoderm. Scale bar = 100µm.

5h 7h 10h

2.5h2h 3h

ExE

EpiVE

ExE

EpiVE

A

B