Upload
others
View
11
Download
0
Embed Size (px)
Citation preview
Center of Excellence in Cancer Research, Tanta University
(CECR) مركز التميز ألبحاث السرطان بجامعة طنطا
Principles of Flow Cytometry
Opening
CECR Introduction
Steps on Research Stairs
Lab Biosafety
Coffee Break
Introduction for Principles of Flow Cytometry.
Introduction for Clinical Applications of Flow Cytometry
Principles of Flow Cytometry
Opening
CECR Introduction
Steps on Research Stairs
Lab Biosafety
Coffee Break
Introduction for Principles of Flow Cytometry.
Introduction for Clinical Applications of Flow Cytometry
Principles of Flow Cytometry
Opening
CECR Introduction
Steps on Research Stairs
Lab Biosafety
Coffee Break
Introduction for Principles of Flow Cytometry.
Introduction for Clinical Applications of Flow Cytometry
Established Date
June 2013 through a project funded by Science and
Technology development Fund (STDF)
PI: Prof. Mohamed Labib Salem
Affiliation: Tanta University
Budget: 9,657,500 L.E
Duration: 36 Months
Established Date
Prof. Mohamed L.Salem Director, CECR
Prof. Said Hammad Vise Director, CECR
Prof. Mohamed Ramadan Clinical Director, CECR
Prof. Mohamed Attia Director of Flow Cytometry Unit, CECR
Dr. Abdel-Aziz Zidan Director of Proteomic & Genomic Unit, CECR
Ms. Mona F. Zidan Research Assistant
Research Team
Eng. Heba M. Wassfy Coordinator , CECR
Dr. Enas Abdul-Raouf Research Assistant
CECR Mission
Create a rich academic environment for excellence in cancer research to lead the next generation of scientists and empower the discovery and entrepreneurships in cancer prognosis, diagnosis, prevention, and treatment through efficient collaboration with national and
international esteemed investigators.
CECR Goals
CECR GOALS
Training Outreach
Office
Research from bench to
clinic
Independent Researchers
Technical/ Clinical
Consultation
Grants Publications
Patents
Scientific Collaboration
Establish the (Center of Excellence in Cancer Research “CECR”) to perform cutting-edge preclinical and clinical reseacrh.
Correlating immunologic and clinical performance between genomic, transcriptomic and proteomic profiling of circulating tumor cells versus primary tumor cells
Two CECR Main Aims
CECR From Application to Funding
STDF-CSE Call
Proposals Evaluation
Interview & Oral Presentation
Site Visit
146 Project
61 Project
25 Project
Nomination: The BD FACSCanto derives its name from the musical term “bel canto” meaning
operatic singing that focuses on ease and purity of tone with an agile and precise vocal
technique.
Flow Cytometry Unit
• BD FACSCanto II Cytometer
Stem cell enumeration
Detection of minimal
residual disease
BD FACSCanto Applications
Analysis of Leukemias and
Lymphomas
Reticulocyte analysis
Detection of
Immunodeficiency diseases
Detection of HIV infection
Analysis of different types of solid
tumors.
Detection of autoantibodies
Research & Clinical applications of the instrument
BD FACSCanto Applications
The IsoFlux system enriches rare cells from biological samples and prepare them for further analysis , It gives immediate access to high- quality , viable cells in a high density format , ready for use in the application of your choice
Cell Sorting Unit
Cell Sorting Unit
IsoFlux Applications
Enumeration Immunofluorescence Fluorescence in situ
hybridization
Next generation sequencing
Mutation detection Gene expression
Microarray
A high throughput technology that allows detection of thousands of genes simultaneously.
Genomic Unit
Genomic Unit
Microarray Applications
Transiptome mRNA Expression miRNA Expression Splice Variance
Genome Structure DNA Copy number
Genome Variation Single Nucleotide Polymorphism
Copy number Variations
Proteome Protein-Protein Interactions DNA – Protein inreraction
Epigenetics DNA Methylation
Chromatin Structure
Agilent 2100 Bioanlayzer.
It is the industry standard for RNA sample QC and has replaced labor-intensive gel electrophoresis for this application, It is also rapidly replacing gel electrophoresis for DNA fragment analysis and SDS-PAGE analysis of protein samples.
Genomic Unit
Genomic Unit
Bioanalyzer Applications
Whole Cells
RNA
RNA Integrity RNA Qualification Small RNA Sizing
DNA DNA Integrity
DNA Qualification
Protein Protein Integrity
Protein Qualification Protein Purity
Gel Documentation System (Biometra Doc Analyze)
Enables simple documentation of fluorescent and non-fluorescent gels, membranes, blots, and assays with the ability to save images to disk for later quantitative analysis or enhancement for publication.
Genomic Unit
Gene AmpPCR System 9700
It is an automated instrument, specifically designed for the amplification of nucleic acids using the Polymerase Chain Reaction (PCR) process
Genomic Unit
Proteomic Unit
Luminex Applications
Clinical Diagnostics Clinical Research Life Science
Research
Biosurveillance Assay Development Tools
CECR Publications
The Use of Dendritic Cells for Peptide-Based Vaccination in Cancer Immunotherapy. Mohamed L. Salem
Immunostimulatory Effects of Triggering TLR3 Signaling Pathway Implication for Cancer Immunotherapy. Mohamed L. Salem, Said M.
Hammad, Mohamed R. Elshanshory, Mohamed A. Attia, Abdel-Aziz A. Zidan.
G-CSF/anti-G-CSF antibody complexes drive the potent recovery and expansion of CD11b+Gr-1+ myeloid cells without compromising CD8+ T cell immune responses. Mark P Rubinstein,
Mohamed L Salem, Andrew L Doedens, Caitlin J Moore, cody Chiuzan, Guillermo L Rivell, David J Cole and Ananda W Goldrath
CECR Publications
Understanding the biology of ex vivo-expanded CD8 T cells for adoptive cell therapy: role of CD62L Immunol Res. Díaz-Montero CM, Zidan AA, Pallin MF, Anagnostopoulos V, Mohamed L Salem, Wieder E, Komanduri K, Montero AJ, Lichtenheld MG
Immunobiology and signaling pathways of cancer stem cells: implication for cancer therapy. Salem ML, El-Badawy AS, Li Z
Autologous transplantation of CD34+ bone marrow derived mononuclear cells in management of non-reconstructable critical lower limb ischemia. Ismail AM1, Abdou SM, Aty HA, Kamhawy AH, Elhinedy
M, Elwageh M, Taha A, Ezzat A, Salem HA, Youssif S, Salem ML
CECR Publications
IL-12 enhances the function and anti-tumor activity in murine and human CD8+ T cells. Mark P. Rubinstein, Ee Wern Su, Samantha Suriano, Colleen A. Cloud, Kristina Andrijauskaite, Pravin Kesarwani, Kristina M. Schwartz, Katelyn Williams, C. Bryce Johnson, Mingli Li, Gina M. Scurti, Mohamed L. Salem, Chrystal M. Paulos, Shikhar Mehrotra, Elizabeth Garrett-Mayer, Michael I. Nishimura, and David J
Monday 24 February 2014 The 1st Annual Conference of
The Center of Excellence in Cancer Research Next Generation Cancer Research & Diagnosis
In Collaboration with Research Centers of Excellence
(STDF- Affiliated Centers)
CECR Activities
CECR Activities
RNA Extraction Training To CECR Research Team, CECR, Tanta University
Educational Hospital
CECR Activities
RNA Extraction Training To CECR Research Team, CECR, Tanta University
Educational Hospital
CECR Activities
Prof. Mohamed L. Salem’s visit to children Research Institute, Medical University of South Carolina, USA
CECR Activities
Prof. Mohamed L. Salem’s visit to Medial College of Georgia , Georgia Regents University, USA
CECR Activities
Prof. Mohamed L. Salem’s visit to Medial College of Georgia , Georgia Regents University, USA
CECR Activities
Prof. Mohamed L. Salem’s & Dr. Sohaila Galal’s visit to International 2013 Forum of Immunology. Montreal, Canada
CECR Activities
Prof. Mohamed Attia’s Training on FACSCanto Diva7 at BD Biosciences ,Heidelberg-Germany
CECR Activities
Dr. Abdel-Aziz Zidan Training Course on “ Affymetrix Geneship System 3000 7G , Stem Cell Center-Jordan University.
CECR Activities
Dr. Abdel-Aziz Zidan Training Course on “ Affymetrix Geneship System 3000 7G , Stem Cell Center-Jordan University.
CECR Activities
Mona Zidan’s Training course at International Center for Genetic Engineering & Biotechnology ICGEB “ Flow Cytometry & Cell Sorting in Biotechnology & Biomedicine Research” Institut Pasteur de Montevideo, Montevideo, Uruguay
CECR Activities
CECR Team Workshop on “The first workshop on Application of bioinformatics in Molecular Diagnostic Virology” . National Cancer Institute, Cairo University
CECR Activities
Dr. Sohaila Galal’s Workshop on “ Gene Expression on Large Scale Using Microarray and Real time PCR Techniques”. Cairo University Research Park
CECR Students
9 PhD. Students
15 MSc. Students
Adaptive Cell Therapy.
Cancer Biology
Anti Cancer effects of medicinal Plans
Stem Call based therapy
Cancer Stem cells
Immunoregulatory cells