Cellular Analysis

www.enzolifesciences.com

Cellular Analysis

Immunology & Inflammation

Natural Products & Antibiotics

Obesity & Metabolism

Signal Transduction

Immunoassay Kits


Enabling Discovery in Life Science®

Enzo Life Sciences, Inc., a subsidiary of Enzo Biochem, Inc., is organized to lead in the development, production, marketing, and sale of innovative life science research reagents worldwide. Now incorporating the skills, experience, and products of ALEXIS Biochemicals, acquired in 2007, BIOMOL International, acquired in 2008, and ASSAY DESIGNS, acquired in 2009, Enzo Life Sciences provides over 25 years of business experience in the supply of research biochemicals, assay systems and biological reagents “Enabling Discovery in Life Science®”.

Based on a very substantial intellectual property portfolio, Enzo Life Sciences, Inc. is a major developer and provider of advanced assay technologies across research and diagnostic markets. A strong portfolio of labeling probes and dyes provides life science environments with tools for target identifi cation and validation, and high content analysis via gene expression analysis, nucleic acid detection, protein biochemistry and detection, molecular biology, and cellular analysis.

• Genomic Analysis • Cellular Analysis

• Post-translational Modifi cation • Signal Transduction

• Cancer & Immunology • Drug Discovery

In addition to our wide range of catalog products, a complementary range of highly specialized custom services are also offered to provide tailor-made solutions for researchers. These include small molecule organic synthesis, peptide synthesis, protein expression, antibody production and immunoassay development.

International Edition 02/2010

ENZO LIFE SCIENCES, INC.

International Edition

Content

Introduction 4-5

Cellular Analysis 6-15 Apoptosis 7 Cell Cycle 9 DNA Regulation/Transcription 11 Heat Shock Proteins/Chaperones 11 Nitric Oxide & Oxidative Stress 14 Proteasome 15

Immunology & Inflammation 16-27 Immunology 17 Inflammation 23

Natural Products & Antibiotics 28-29

Obesity & Metabolism 30-33

Signal Transduction 34-41

Alphabetical Index 42

International Distributors 43

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ELISA & Enzyme Immunoassay KitsEnzo Life Sciences offers a large variety of immunoassay kits in a variety of formats. As scientists and manufacturers of kits, we understand the critical nature of your research. Each kit is put through rigorous testing to ensure high precision, accuracy, sensitivity, and specifi city. You can be confi dent that you will obtain reproducible results, day-after-day and lot-after-lot.

Our kits offer:

Specifi city• – low reactivity with structurally related molecules

Flexibility• – adaptable to automated equipment for higher throughput systems

Consistency• – reproducible results, lot-after-lot

Sensitivity• – better quantifi cation thresholds than other methods FIGURE: The graph above demonstrates the robust and reproducible nature of the IL-6 (human) EIA Kit, showing standard curves from 5 lots manufactured over 5 years.

Competitive Immunoassay

Basic Immunoassay Formats

In a competitive immunoassay the antigen in a sample com-petes

a) for limited antibody binding sites with antigen conju-gated to a reporter enzyme (see Figure), or

b) for limited antibody binding sites in an antigen-coated well (not shown).

This produces an inverse relationship between antigen con-centration and substrate turnover.

Sandwich Immunoassay

Indirect Immunoassay

In a immunometric or “sandwich” assay two antibodies spe-cific to the antigen are used to capture or “sandwich” the antigen in the well for detection. This assay exhibits a direct correlation between antigen concentration and substrate response.

The indirect immunoassay uses a solid phase coated with antigen to detect or quantitatively determine antibody in samples. This format is commonly used to detect the pres-ence of antibodies to specific antigenic sites on viruses.

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ELISA Development KitsIn addition to the large range of immunoassay kits we present a portfolio of ELISA development kits called “ImmunoSet™” or “detection sets [for ELISA application]”. These products offer an economical way to get the most out of your research budget. Our ELISA develop-ment kits provide sensitive, precise, and reliable quantifi cation in multiple complex sample matrices such as cell lysates, serum, plasma, and tissue.

• Sensitivity – the limit of detection is determined using data from multiple assays

• Specifi city – potential reactivity to related molecules and species is evaluated

• Dilutional linearity – the minimum recommended dilution to eliminate matrix interference in the assay is determined

• Parallelism – we ensure the recombinant standard used in the assay refl ects the native analyte in biological samples

We are continually developing new kits and expanding our kit portfolio to meet your research needs. To see our current portfolio of kits, visit www.enzolifesciences.com.

Custom Immunoassay ServicesEnzo Life Sciences now offers custom assay development services utilizing the more than 17 years of industry-proven design and manufac-turing capabilities of Assay Designs®. This expertise provides our customers with a complete scope of industry-proven assay development services from project inception to completion. Our list of satisfi ed customers includes clients from industry, government, and academia.

Our assay development scientists possess extensive experience in commercial immunoanalytical assays including colorimetric, fl uores-cent, chemiluminescent, and fl uorescence polarization detection systems. Our capabilities extend to a wide range of analytes from small molecules to large proteins and their various post translational modifi cations, as well as bead-based multiplex analyte detection for fl ow cytometry.

Our assay expertise includes:

Small and large molecule non-radioactive detection•

Plate-base single analyte EIA/ELISA•

Bead-based multiplex platform for fl ow cytometry•

Heterogeneous and homogeneous formats•

Biomarker assay development•

Enzyme activity assays•

Complex specifi c antibody pairs•

Choose your detection system:

Colorimetric•

Fluorescent•

Chemiluminescent•

Fluorescence Polarization•

Custom assays are characterized and validated based upon fi t-for-purpose validation criteria for the customer’s specifi c application in animal or human biological matrices. Proven reagent production and manufacturing capabilities assure smooth scale-up of projects from smaller studies to large clinical studies.

Our diverse group of immunoassay scientists has experience with assay platforms beyond those mentioned above. If you are interested in an assay format that is not mentioned, please contact us and we will work to accommodate your request.

Learn more about our services and submit your custom immunoassay project requirements by visiting www.enzolifesciences.com.

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Cellular Analysis

Apoptosis•

Cell Cycle•

DNA Regulation/Transcription•

Heat Shock Proteins/Chaperones•

Nitric Oxide & Oxidative Stress•

Proteasome•

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Bax

Bcl-2

Cytochrome c

M30 CytoDeath™ ELISA Kit

Bax is an approx. 22 kDa pro-apoptotic protein belonging to the Bcl-2 family of proteins, which are characterized by the presence of at least one Bcl-2 homology (BH) domain. Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes where it inserts and mediates the release of cytochrome c. This activates caspase-3 and perpetuates the apoptotic cascade.

The Bcl-2 family of proteins regulate the intrinsic apoptotic pathway and are characterized by the sharing of at least one of four conserved Bcl-2 homology (BH) domains. Bcl-2 family members function as either pro- or anti-apoptotic factors, and are generally categorized into three groups based on structural homology and function. Bcl-2-like proteins (Bcl-2, Bcl-xL, Bcl-w, A1, and Mcl-1) are pro-survival. Pro-apoptotic members can be subdivided into two groups: the multi-BH domain group, including Bax, Bak, and Bok; and the more numerous and diverse BH3-only group, which includes Bim, Bad, and Bid.

Cytochrome c, which is a critical component of the electron transport chain, is a component of the functional apoptosome. Upon release from the mitochondria, cytochrome c binds to the adapter proteins Apaf-1 and procaspase-9 to form a cytosolic apoptosome complex. This event results in activation of procaspase-9 and its subsequent cleavage and activation of caspase-3 leading to the degradation of a series of cellular substrates and subsequently cell death.

The M30 CytoDeath™ ELISA kit is a unique biomarker assay that allows the determination of epithelial cell apoptosis in cell culture. The as-say is based on the unique M30 CytoDeath™ antibody which recognizes a neoepitope of cytokeratin 18 (CK18) formed only after caspase cleavage. The sensitivity and range of the assay has been optimized to suit in vitro applications. Overall the kit represents a versatile tool that also allows the determination of epithelial apoptosis in multicellular spheroids and organ culture systems.

Bax (human), EIA kitADI-900-138 1 x 96 wells

Bcl-2 (human), EIA kitADI-900-133 1 x 96 wells

Cytochrome c (human), EIA kitADI-900-141 1 x 96 wells

M30 CytoDeath™ ELISA kit ALX-850-336-KI01 1 Kit

Species Reactivity Human

Sample Type Cell lysates

Sensitivity 10.1 pg/ml

Range 62.5 – 2,000 pg/ml




Range 18.8 – 1,200 pg/ml




Range 28.13 – 900 pg/ml

Species Reactivity Human, monkey and bovine

Sample TypeCell lysates or culture supernatants from CK18 positive (epithelial) apoptotic cells or tissues. Not suitable for serum or plasma samples.

Sensitivity 60 U/l, Standard Z (0 U/l) + 3 S.D.

Range 250 – 3,000 U/l

APOPTOSIS

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M30-Apoptosense® ELISA KitThe M30 Apoptosense® ELISA kit is a unique biomarker assay that allows the determination of epithelial cell apoptosis serum and plasma. The assay is based on the unique M30 CytoDeath™ antibody which recognizes a neoepitope of cytokeratin 18 (CK18) formed only after caspase cleavage. The sensitivity and range of the assay has been optimized to suit both in vitro and in vivo applications. Overall the kit detects tumor and live cell apoptosis in human blood samples and is specifi c for human tumor apoptosis in xenograft models.

M30-Apoptosense® ELISA kit ALX-850-270-KI01 1 Kit ALX-850-270-5001 5 x 1 Kit


Sample TypeSerum or plasma (EDTA, citrate, heparin plasma) samples containing caspase-cleaved CK18 from apoptotic epithelial cells. Cell lysates or culture supernatants from CK18 positive (epithelial) apoptotic cells or tissues.

Sensitivity 25 U/l, Standard A (0 U/l) + 2 S.D.

Range 75 – 1 000 U/l

M65® ELISA Kit

Netrin-4

The M65® ELISA kit is primarily intended for quantitative determination of total epithelial cell death (apoptosis and necrosis). Can be used in combination with the M30-Apoptosense® ELISA kit (Prod. No. ALX-850-270) and/or the M30 CytoDeath™ ELISA kit (Prod. No. ALX-850-336) to allow the calculation of apoptosis:necrosis ratios.

The netrins are a family of laminin-related secreted proteins. Involved in axonal guidance, they are required for regeneration and mainte-nance of the central nervous system and have been implicated in the development of mammary gland, lung, pancreas, and blood vessel. Netrin-4 acts during the development and controls the migration of neurons, being chemoattractive for some neurons and chemorepellent for others. Netrin-4 is broadly expressed in both neural and non-neural tissues and promotes angiogenesis by stimulating proliferation and migration of microvascular endothelial cells to the same extent that vascular endothelial growth factor (VEGF).

M65® ELISA kit ALX-850-310-KI01 1 Kit


Sample TypeSerum or plasma (EDTA, citrate, heparin plasma) samples containing caspase-cleaved CK18 from apoptotic epithelial cells. Culture supernatants from CK18 positive (epithelial) apoptotic cells or tissues.

Sensitivity 11 U/l, Standard A (0 U/L) + 2 S.D.

Range 125 – 2 000 U/l

Product Species Reactivity Sample Type Sensitivity Range Prod. No. Size

Netrin-4 (human), ELISA kit Human Culture supernatants, plasma and serum

50 pg/ml 78 – 5,000 pg/ml APO-54N-037-KI01 1 x 96 wells

Netrin-4 (human), detection set [for ELISA application]

Human Culture supernatants, plasma and serum

50 pg/ml 0 – 5 ng/ml APO-54N-033-KI01 5 x 96 wells

Smac/DIABLOSmac/DIABLO is one of the pro-apoptotic proteins normally found in the intermembrane space of the mitochondria. In response to apoptotic stimuli, Smac/DIABLO is released into the cytosol resulting in permeabilization of the mitochondrial membrane. Cytosolic Smac/DIABLO activates caspase-9 by binding IAP proteins. Changes in the expression level of Smac/DIABLO have been demonstrated in a number of human cancers including melanoma, ovary and prostate.

Smac/DIABLO (human), EIA kitADI-900-125 1 x 96 wells




Range 31.25 – 1,000 pg/ml

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XIAP

Survivin

XIAP (X-linked inhibitor of apoptosis) belongs to the IAP (inhibitors of apoptosis) family, which is characterized by one or more BIR (baculovirus IAP repeat) domains. These proteins have multiple biological functions that include binding and inhibiting caspases, regulating cell cycle progression and modulating receptor-mediated signal transduction. XIAP functions by direct binding and inhibi-tion of several caspases, including caspase-3, caspase-7 and caspase-9.

Survivin is the smallest inhibitor of apoptosis (IAP) so far identifi ed (16.5 kDa). It is involved in the inhibition of apoptosis and cell division. Survivin regulates the G2/M phase of the cell cycle by as-sociating with the mitotic spindle microtubules and directly inhibits caspase-3 and caspase-7. Its expression has been reported at high levels in embryonic tissues, but at low or non-detectable levels in normal tissue. Survivin is selectively expressed in the most common human cancers and is associated with clinical tumor progression. It has been proposed that survivin may be used as a universal tumor antigen for immunotherapy.

XIAP (human), EIA kitADI-900-124 1 x 96 wells

Survivin (human), EIA kitADI-900-111 1 x 96 wells




Range 312.5 – 10,000 pg/ml


Sample Type Cell lysates, culture supernatants, plasma, serum and urine

Sensitivity 4 pg/ml

Range 31.25 – 1,000 pg/ml

ClusterinClusterin is a secretory heterodimeric disulfi de-linked glycoprotein containing 449 amino acids that is expressed in virtually all tissues, and found in all human fl uids. It is involved in numerous physi-ological processes important for carcinogenesis and tumor growth, including apoptotic cell death, cell cycle regulation, DNA repair, cell adhesion, tissue remodeling, lipid transportation, membrane recycling, and immune system regulation.

Clusterin (human), competitive ELISA kit AG-45A-0013EK-KI01 1 x 96 wellsAG-45A-0013PP-KI01 Twin Plex 2 x 96 wellsAG-45A-0013TP-KI01 Penta Plex 5 x 96 wells


Sample Type Culture supernatants, plasma and serum

Sensitivity 1 ng/ml

Range 0.001 – 5 µg/ml

CELL CYCLE

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p27/Kip1p27/Kip1 is a member of the CDK inhibitors that binds to cyclin/CDK complexes and arrests cell division. In general, p27/Kip1 expression is highest in quiescent cells and falls as cells reenter the cell cycle. Several anti-proliferative signals, including cell-to-cell contact, the withdrawal of cytokines, the withdrawal of mitogens, and exposure to cAMP result in p27/Kip1 accumulation.

p27/Kip1 (human), EIA kitADI-900-139 1 x 96 wells




Range 25 – 1,600 pg/ml

p21Human p21, a CDK inhibitor (cyclin-CDK-2 and cyclin-CDK4) is regulating cell cycle progression in G1 phase, and is involved in terminal differentiation and apoptosis. p21 expression can be induced independently of p53 by TGF-β, tamoxifen, progesterone, nerve growth factor, and apicidin. Human p21 expression has been implicated in a variety of human cancers, including those of the prostate, bladder, and esophagus.

p21 (human), EIA kit ADI-900-161 1 x 96 wells




Range 15.6 – 1,000 pg/ml

p53

Pin1

The p53 tumor suppressor protein is a transcription factor that regulates a variety of biological functions including growth arrest, ap-optosis, DNA repair, and differentiation. MDM2 is the principal antagonist of p53. It acts as an E3 ubiquitin ligase, subjecting p53 in the cytoplasm and nucleus to proteosomal degradation. Various strategies for cancer therapy involve activating the apoptotic and growth suppressive functions of p53 by disrupting its association with MDM2.

Pin1 is a member of the parvulin family within the peptidyl-prolyl cis-trans isomerase (PPIase) group of proteins. It recognizes and binds to a specifi c motif of phosphorylated serine or threonine resi-dues preceding a proline and catalyzes the cis-trans isomerisation. Pin1 is involved in many basic cellular processes including cell-cycle regulation, transcription, differentiation, and proliferation. It has been implicated in neurodegenerative disorders such as Alzheimer’s disease, and is overex-pressed in many human cancers.

Pin1 EIA kitADI-900-146 1 x 96 wells

Species Reactivity Human and mouse



Range 62.5 – 2,000 pg/ml


p53 EIA kit Human, mouse and rat Cell lysates, plasma, serum and tissue

9 pg/ml 0 – 870 pg/ml ADI-900-117 1 x 96 wells

ImmunoSet™ p53/MDM2 Complex ELISA development set

Human, mouse and rat Cell lysates 0.35 ng/ml 0.78 – 50 ng/ml ADI-960-070 5 x 96 wells

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DNA DamageExposure of cells to oxidative and environmental stresses frequently results in the breakdown or oxidation of genomic DNA. Assays to evaluate the integrity of genomic DNA, or to assess the pres-ence of oxidized DNA are frequently used as a means of verifying the onset of apoptosis or DNA damage.

DNA Damage EIA kitADI-EKS-350 1 x 96 wells

Species Reactivity Species independent

Sample Type Serum, saliva and urine

Sensitivity 0.59 ng/ml

Range 1.875 – 60 ng/ml

MDA5

RIG-I

αB-Crystallin

The innate immune system initially recognizes RNA virus infection and evokes antiviral responses by producing type I interferons (IFNs). Recognition of the viral RNA is mediated by either the two cyto-plasmic RNA helicases retinoic acid inducible gene-1 (RIG-I; Ddx58) and melanoma differentiation associated gene 5 (MDA5; Helicard; Ifi h1) or by Toll-like receptor 3 (TLR3) located to an endosomal-like compartment. The physiological agonist of MDA5 has still to be discovered. MDA-5 is an early response gene inducible by IFN and TNF-α, responding predominantly to IFN-β.

RIG-I (retinoic acid-inducible gene I; Ddx58), a member of the DEAD-box RNA helicases, is activated by cytoplasmic dsRNA and 5’-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adapter inducing IFN-β(Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation.

MDA5 (human) (intracellular), ELISA kitAPO-54N-039-KI01 1 x 96 wells


Sample Type Cell extracts




RIG-I (human) (intracellular), ELISA kit Human Cell extracts 0.1 ng/ml 0.156 – 10 ng/ml APO-54N-045-KI01 1 x 96 wells

RIG-I (human) (intracellular), detection set [for ELISA application]

Human Cell extracts 0.1 ng/ml 0.156 – 10 ng/ml APO-54N-038-KI01 5 x 96 wells

α-Crystallins composed of ~20 kDa αA and αB subunits are members of the small heat shock protein (sHSP) family. They function as major water-soluble proteins accounting for almost 50% of total protein in the mammalian transparent eye lens, also existing in a variety of other tissues. α-Crystallins possess structural and functional similarities and share sequence homology with HSP25/27. The conserved α-crystallin domain participates in oligomer assembly which is critical to their function in the prevention of irreversible protein aggregation.

ImmunoSet™ αB-Crystallin ELISA development setADI-960-074 5 x 96 wells

Species Reactivity Human, mouse, rat and cow




DNA DAMAGE

HEAT SHOCK PROTEINS/CHAPERONES

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Grp75Glucose-regulated 78 kDa protein (Grp75), also known as Mortalin or HSPA9, is a member of the HSP70 family. It resides in the mitochondrial matrix where it collaborates with HSP60 in the re-folding of proteins translocated into this organelle. Like its E. coli homolog DnaK, Grp75 possesses a cation-dependent ATPase activity considered central to its function as a molecular chaperone. Grp75 has been implicated in various cancers, cardiovascular disease, neurodegeneration, and aging.

ImmunoSet™ Grp75 ELISA development setADI-960-143 5 x 96 wells

Species Reactivity Human, mouse and rat



Range 3.125 – 100 ng/ml

Grp94Glucose-regulated protein 94 (Grp94) is an abundant resident endoplasmic reticulum (ER) lumenal protein, belonging to the HSP90 family of molecular chaperones. Its expression is upregulated by stress conditions such as glucose starvation and heat shock, which promote protein misfolding or unfolding. In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular traffi cking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells.

ImmunoSet™ Grp94 ELISA development setADI-960-077 5 x 96 wells

Species Reactivity Human, mouse, rat and dog

Sample Type Cell lysates and tissue



HSP25HSP25 is a member of the family of small heat shock proteins (sHSPs). The sHSPs are related to the α-crystallins, and generally exist as large oligomeric complexes that serve to stabilize and assist in refolding of denatured proteins. HSP25 is highly homologous to the human HSP27, and its expres-sion is seen in many species, including mouse, rat, bovine, canine, and hamster cells.

ImmunoSet™ HSP25 (rodent), ELISA development setADI-960-075 5 x 96 wells

Species Reactivity Mouse and rat




HSP27HSP27 is a chaperone of the small heat shock protein (sHSP) group among ubiquitin, α-crystallin, HSP20 and others. HSP27 has been shown to inhibit actin polymerization by binding of unphosphorylated HSP27 monomers to actin intermediate fi laments. Anti-apoptotic functions of HSP27 have also been identifi ed through interactions with DAXX7, activation of Akt and inhibition of apoptosome formation. Evidence suggests altered expression of HSP27 is implicated in the pathogenesis of breast, ovarian and prostate cancer.


HSP27 (human), EIA kit Human Cell lysates, plasma, serum and tissue

0.39 ng/ml 0.39 – 25 ng/ml ADI-EKS-500 1 x 96 wells

ImmunoSet™ HSP27 high sensitivity (human), ELISA development set

Human Cell lysates, plasma and serum

97 pg/ml 0.1 – 3.2 ng/ml ADI-960-076 5 x 96 wells

[pSer15]HSP27 (human), EIA kit Human Cell lysates, plasma and serum

10.15 pg/ml 31.25 – 1,000 pg/ml ADI-900-170 1 x 96 wells

[pSer78]HSP27 (human), EIA kit Human Cell lysates, plasma and serum


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HSP40

HSP60

HSP40/Hdj1 is a cytosolic co-chaperone belonging to the class DnaJ. HSP40 functions in protein folding by binding nascent peptides and unfolded substrates and facilitating substrate interaction with HSP70. Binding of HSP40 to HSP70 increases HSP70 ATP hydrolysis and substrate binding, which is reversed by the action of nucleotide exchange factors (GrpE in E. coli). Repeated cycles of HSP40/HSP70 peptide binding and ATP hydrolysis prevent premature folding and aggregation, promoting protein maturation throughout the cell.

HSP60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. HSP60 is the eukaryotic homolog of the E. coli GroEL protein. It forms a multimeric complex in the mito-chondria with HSP10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place.

ImmunoSet™ HSP40 (human), ELISA development setADI-960-073 5 x 96 wells






HSP60 (human), EIA kit Human Cell lysates, serum and tissue


Anti-HSP60 IgG/A/M (human), ELISA kit Human Serum 2.88 ng/ml 7.81 – 250 ng/ml ADI-EKS-650 1 x 96 wells

HSP70HSP70 family members contain highly conserved N-terminal ATPase and C-terminal protein binding domains. Binding of peptide to HSP70 is assisted by HSP40, and stimulates the inherent ATPase activity of HSP70, facilitating ATP hydrolysis and enhanced peptide binding. HSP70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the refolding of misfolded or denatured proteins, traffi cking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.


HSP70 EIA kit Human, mouse and rat Cell lysates and tissue

200 pg/ml 780 – 50,000 pg/ml ADI-EKS-700B 1 x 96 wells

HSP70 high sensitivity EIA kit Human, mouse and rat Plasma and serum 90 pg/ml 0.20 – 12.5 ng/ml ADI-EKS-715 1 x 96 wells

Anti-HSP70 IgG/A/M (human), ELISA kit Human Serum 6.79 ng/ml 31.25 – 1,000 ng/ml ADI-EKS-750 1 x 96 wells

HSP70B' EIA kit Human Cell lysates, serum and tissue

62 pg/ml 0.156 – 10 ng/ml ADI-EKS-725A 1 x 96 wells

HSP90The HSP90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones. HSP90 dimers function in cooperation with co-chaperones (e.g. HSP40, HSP70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential bind-ing and hydrolysis of ATP by HSP90 is inhibited by ansamycin drugs (e.g. geldanamycin (Prod. No. BML-EI280), 17-AAG (Prod. No. BML-EI308)) which occupy the N-terminal HSP90 nucleotide-binding pocket. Many HSP90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making HSP90 inhibitors useful anticancer agents.

HSP90α (human), EIA kitADI-EKS-895 1 x 96 wells


Sample Type Cell lysates, serum and tissue

Sensitivity 50 pg/ml

Range 62.5 – 4,000 pg/ml

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HO-1Heme Oxygenase-1 (HO-1) also known as HSP32, catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.


HO-1 (human), EIA kit Human Cell lysates and tissue


ImmunoSet™ HO-1 (human), ELISA development set

Human Cell lysates and tissue


ImmunoSet™ HO-1 (mouse), ELISA development set

Mouse Cell lysates, plasma, serum and tissue


HO-1 (rat), EIA kit Rat Cell lysates, plasma, serum and tissue

0.036 ng/ml 0.195 – 12.5 ng/ml ADI-EKS-810A 1 x 96 wells

ImmunoSet™ HO-1 (rat), ELISA development set

Rat Cell lysates, plasma, serum and tissue


ADMAAsymmetric dimethylarginine (ADMA) is an endogenous inhibitor of nitric oxide (NO) synthesis. NO is one of the major endothelium-derived vasoactive mediators and is involved in the modulation of blood fl ow and blood pressure. Even small changes of ADMA concentration alter vascular NO production. ADMA is a novel cardiovascular risk factor. Elevated ADMA concentrations are found in patients with diabetes mellitus, hypercholesterolemia, hypertension, peripheral arterial disease, chronic renal failure, preeclampsia or erectile dysfunction.


ADMA (human), ELISA kit Human EDTA-plasma and serum

0.05 µmol/l 0 – 10 µmol/l ALX-850-323/1-KI01 1 x 96 wells

ADMA Direct, (mouse/rat) ELISA kit Mouse and rat EDTA-plasma and serum

0.05 µmol/l 0 – 10 µmol/l ALX-850-327-KI01 1 x 96 wells

SDMA

GPX3

Symmetric dimethylarginine (SDMA) is a methylated derivative of L-Arginine which is strictly elimi-nated by renal extraction, thus SDMA plasma level is strongly correlated to renal function. SDMA exhibits properties of a reliable marker of renal dysfunction. Moreover, there are hints that increased SDMA correlates with total sequential organ failure indicating both renal and hepatic failure and an increased cardiovascular risk.

Glutathione peroxidase (GPX) catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced gluta-thione and functions in the protection of cells against oxidative damage. This enzyme is found mainly in the cytosol of mammalian cells. Since redox control has been implicated in the cause of metabolic dysfunction, plasma or serum measurement of GPX3 may give some benefi ts to the diagnosis of these metabolic diseases.

SDMA (human), ELISA kitALX-850-331-KI01 1 x 96 wells


Sample Type EDTA plasma and serum

Sensitivity 0.05 µmol/l

Range 0.1 – 2 µmol/l


GPX3 (human), ELISA kit Human Culture supernatants, plasma and serum

100 pg/ml 0 – 64 ng/ml AG-45A-0020EK-KI01AG-45A-0020TP-KI01AG-45A-0020PP-KI01

1 x 96 wells2 x 96 wells5 x 96 wells

GPX3 (mouse), ELISA kit Mouse Culture supernatants, plasma and serum



NITRIC OXIDE & OXIDATIVE STRESS

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MyeloperoxidaseMyeloperoxidase (MPO), a tetramer, consisting of 2 light chains and 2 heavy chains, is derived from a single chain precursor. As a response to microbial invasion, MPO is released from the cytoplasmic granules of neutrophils into the extracellular space and phagosome, MPO can be used as a marker of airway infl ammation caused either by disease, such as asthma, or by environmental irritants.

Myeloperoxidase (human), EIA kitADI-900-115 1 x 96 wells


Sample Type Culture supernatants, nasal lavage, plasma, sputum and urine


Range 0.195 – 12.5 ng/ml

Protein Carbonyl ELISA KitReactive oxygen species (ROS) are produced as a consequence of normal aerobic metabolism. They cause cellular damage, an important part of which is the oxidation of amino acid residues on proteins, forming protein carbonyls. Therefore the most general indicator and the most commonly used marker of protein oxidation is the protein carbonyl content. Protein carbonyls are formed by a variety of oxidative mechanisms and are sensitive indices of oxidative injury.

Protein carbonyl ELISA kitALX-850-312-KI01 1 x 96 wells


Sample Type Plasma, other fluids, cell and tissue extracts

Sensitivity n/a

Range 0 – 1 nmol/mg

ProteasomePROTEASOME

PDIThe mammalian PDI (protein disulfi de-isomerase) family encompasses several highly divergent pro-teins which are involved in the processing and maturation of secretory proteins in the endoplasmic reticulum by catalyzing the rearrangement of disulfi de bonds.

ImmunoSet™ PDI ELISA development setADI-960-072 5 x 96 wells


Sample Type Cell lysates, culture supernatants, plasma and tissue



Proteasomes are often overexpressed in cancer cells; abnormally high expression of proteasomes having been found in human leukaemia cells, renal cancer cells and in breast cancer cell lines. In patients suffering from autoimmune diseases, malignant myelo-proliferative syndromes, multiple myeloma, acute and chronic lymphatic leukaemia, solid tumor, sepsis or trauma, the concentration of blood plasma 20S proteasome (known as circulating proteasome) has been found to be elevated, to correlate with the disease state, and may have prognostic signifi cance.

Proteasome ELISA kitBML-PW0575-0001 1 x 96 wells


Sample Type Cell lysates, plasma, serum and tissue extracts

Sensitivity 0.025 µg/ml

Range 0.025 – 1.6 µg/ml

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Immunology & Infl ammation

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IMMUNOLOGYBAFF

IFN-γ

BAFF (B lymphocyte activating factor of the tumor necrosis factor family) is a vital homeostatic cytokine for B cells that helps regulate both innate and adaptive immune responses. BAFF is a master regulator of peripheral B cell survival and co-stimulates activated T cells. In humans increased serum levels of BAFF are found in a number of different autoimmune diseases, such as rheumatoid arthritis, Sjögren’s syndrome and systemic lupus erythematosus, and BAFF is found in infl ammatory sites in which there is lymphoid neogenesis. BAFF levels are also elevated in patients with multiple myeloma and B cell chronic lymphoid leukemia (B-CCL).

Human interferon-γ (IFN-γ) is an infl ammatory cytokine secreted by T lymphocytes and natural killer (NK) cells. IFN-γ is primarily characterized as an activator of macrophages and is the principle cytokine produced by CD4+ infl ammatory Th1 cells. IFN-γ signals are transmitted via a heterodimeric IFNGR1/IFNGR2 receptor which activates transcription of immunoregulatory genes via the Jak/Stat pathway.


IFN-γ (human), EIA kit Human Culture supernatants, plasma, serum and urine


IFN-γ (mouse), EIA kit Mouse Culture supernatants, plasma, serum and urine

< 10 pg/ml 37 – 3,000 pg/ml ADI-900-137 1 x 96 wells


BAFF (soluble) (mouse), ELISA kit

Mouse Culture supernatants, plasma and serum

0.2 ng/ml 0 – 20 ng/ml APO-54N-019-KI01 1 x 96 wells

BAFF (soluble) (mouse), detection set [for ELISA application]

Mouse Culture supernatants and serum


GRO/CINC-1GRO/CINC-1 (growth related oncogene/cytokine-induced neutrophil chemoattractant-1) is a mem-ber of the a (CXC) subfamily of chemokines. It is the rat homolog to human interleukin-8 (IL-8). Three additional rat CXC chemokines (CINC-2a, CINC-2b, CINC-3/MIP-2) have been identifi ed. CINCs appear to be the rat counterpart of human GROs.

GRO/CINC-1 (rat), EIA kitADI-900-074 1 x 96 wells

Species Reactivity Rat



Range 4.7 – 300 pg/ml

IgGImmunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. Isotyping kits can be used to quantify the concentration of mono-clonal antibodies or determine the isotype of a specifi c clone.


IgG1 (mouse), EIA kit Mouse Culture supernatants and serum 1.79 ng/ml 7.81 – 250 ng/ml ADI-900-109 1 x 96 wells

IgG2a (mouse), EIA kit Mouse Culture supernatants and serum 318.8 pg/ml 7.81 – 250 ng/ml ADI-900-113 1 x 96 wells

IgG2b (mouse), EIA kit Mouse Culture supernatants and serum 1.05 ng/ml 7.81 – 250 ng/ml ADI-900-110 1 x 96 wells

IgG3 (mouse), EIA kit Mouse Culture supernatants and serum 0.901 ng/ml 7.81 – 125 ng/ml ADI-900-121 1 x 96 wells

ComplementActivation of the complement cascade is initiated by multiple pathways, including C1q binding of antigen-antibody complexes on patho-gens (classical pathway), binding of serum lectin to pathogen-unique mannose residue patterns (MBL pathway), or the spontaneous hydrolysis of C3 and subsequent processing to C3 convertase (alternative pathway). Complement activation is associated with sepsis, respiratory distress syndrome, multi-organ failure and transplant organ rejection.


Complement C3a des Arg (human), EIA kit

Human EDTA plasma 0.120 ng/ml 0.313 – 20 ng/ml ADI-900-058ADI-901-058

1 x 96 wells5 x 96 wells

Complement C4a des Arg (human), EIA kit

Human EDTA plasma 0.76 ng/ml 0.78 – 200 ng/ml ADI-900-059ADI-901-059


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IL-4Interleukin-4 (IL-4) is a cytokine produced by activated T cells and mast cells and functions in the activation of B cells, regulation of the IgE class switch and increases MHC II expression.


IL-4 (human), EIA kit Human Culture supernatants, serum and urine

< 2 pg/ml 10.24 – 400 pg/ml ADI-900-145 1 x 96 wells

IL-4 (mouse), EIA kit Mouse Culture supernatants and serum


IL-1βThe interleukin-1 (IL-1) cytokine family consists of IL-1α, IL-1β, and IL-1Rα (receptor antagonist) which are expressed primarily by en-dothelial cells and activated macrophages. IL-1α and IL-1β elicit pro-infl ammatory cytokine production and cellular activation, and are the primary mediators of hypothalamic regulation that results in fever.


IL-1β (human), EIA kit Human Plasma, serum and urine < 1 pg/ml 10.24 – 400 pg/ml ADI-900-130 1 x 96 wells

IL-1β (mouse), EIA kit Mouse Culture supernatants, plasma and serum

< 3 pg/ml 15.6 – 1,000 pg/ml ADI-900-132 1 x 96 wells

IL-1β (rat), EIA kit Rat Culture supernatants, plasma and serum

< 12 pg/ml 25.6 – 2,500 pg/ml ADI-900-131 1 x 96 wells

IL-2Interleukin-2 (IL-2) plays a critical role in the mediation of immune response by acting as a growth and differentiation factor for T cells, as well as B cells, natural killer (NK) cells, lymphokine-activated killer (LAK) cells, tumor-infi ltrating lymphocytes (TIL), monocytes, macro-phages and oligodendrocytes. The human IL-2 receptor can be composed of several combinations of a 55 kDa IL-2Rα subunit, a 70-75 kDa IL-2Rβ; subunit and a 64 kDa IL-2Rγ subunit.


IL-2 (human), EIA kit Human Culture supernatants, plasma and serum

6.6 pg/ml 7.81 – 500 pg/ml ADI-900-118AADI-901-118ADI-902-118


10 x 96 wellsIL-2 (mouse), EIA kit Mouse Culture supernatants and

serum3.12 pg/ml 7.81 – 1,000 pg/ml ADI-900-042 1 x 96 wells

IgMImmunoglobulin M (IgM) is an immunoglobulin expressed initially on the surface of B cells as a monomer of two mu heavy chains and two light chains. Following B cell activation, IgM is secreted in its pentameric form, consisting of fi ve IgM molecules joined at the base of the Fc domain by a J chain. IgM is the fi rst Immunoglobulin expressed following infection.

IgM (mouse), EIA kitADI-900-120 1 x 96 wells

Species Reactivity Mouse

Sample Type Ascites fluid, culture supernatants and serum



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IL-6

IL-8

Interleukin-6 (IL-6) is a cytokine critical to the regulation of the immune and hematopoietic systems. Human IL-6 is expressed by T cells, mast cells, monocytes, macrophages, fi broblasts, endothelial cells, keratinocytes and many tumor cell lines. It appears to take part in acute phase reactions and response to injury and infl ammation.

Interleukin-8 (IL-8) is a cytokine produced by monocytes, macrophages, neutrophils, lymphocytes, dermal fi broblasts, keratinocytes, vascular endothelial cells, melanocytes, hepatocytes and various tumor cell lines. It is also a member of the CXC family of chemokines, displaying chemotactic activ-ity for neutrophils and T cells.

IL-8 (human), EIA kitADI-900-156 1 x 96 wellsADI-901-156 5 x 96 wellsADI-902-156 10 x 96 wells




Range 7.8 – 1,000 pg/ml


IL-6 (human), EIA kit Human Culture supernatants, plasma, serum and urine

6.01 pg/ml 7.81 – 500 pg/ml ADI-900-033ADI-901-033ADI-902-033


10 x 96 wellsIL-6 (mouse), EIA kit Mouse Culture supernatants and serum 1.01 pg/ml 7.81 – 1,000 pg/ml ADI-900-045 1 x 96 wells

IL-10Interleukin-10 (IL-10), a macrophage deactivating factor, was initially identifi ed as the cytokine synthesis inhibitory factor based on the observation that mouse Th1 cytokine production was suppressed by a Th2-derived factor. It also regulates growth and/or differentiation of B cells, mast cells, granulocytes, dendritic cells, keratinocytes and endothelial cells. Circulating levels of IL-10 are increased in allergic asthma, systemic sclerosis, a variety of cancers, post-transplantation patients, and in sepsis. The therapeutic potential of IL-10 includes rheumatoid arthritis, systemic lupus erythematosus, and multiple sclerosis.


IL-10 (human), EIA kit Human Culture supernatants, plasma and serum

3.75 pg/ml 7.81 – 500 pg/ml ADI-900-036ADI-901-036ADI-902-036


10 x 96 wellsIL-10 (mouse), EIA kit Mouse Culture supernatants and serum < 12 pg/ml 37 – 3,000 pg/ml ADI-900-148 1 x 96 wells

IL-33IL-33 is a new member of the IL-1 family. It is involved in T helper type 2 response via the activation of its receptor ST2 (IL-1RL1) driving the production of Th2-associated cytokines. The stimulation of ST2 by IL-33 induces the NF-κB and MAPK pathways. IL-33 is synthesized as a precursor, which is cleaved by caspase-1 to its 18 kDa mature and active form.

IL-33 (soluble) (human), detection set [for ELISA application]APO-54N-025-KI01 5 x 96 wells


Sample Type Culture supernatants and serum

Sensitivity 5 pg/ml

Range 0 – 500 pg/ml

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LIGHT

MCP-1

LIGHT (derived from “homologous to Lymphotoxin”) belongs to the TNF superfamily and is bound by three receptors: lymphotoxin receptor (LTR), herpesvirus entry mediator (HVEM), and decoy re-ceptor 3 (DcR3). LIGHT triggers several signaling pathways and is able to differentiate embryonic stem cells. It suppresses tumor formation in vivo by inducing apoptotic cell death and induces a proinfl ammatory state in vascular endothelial cells. Mice overexpressing LIGHT spontaneously develop severe autoimmune disease. LIGHT has been reported to increase in rheumatoid arthritis patients and to induce a RANKL dependent and -independent osteoclasts formation.

Monocyte chemoattractant protein-1 (MCP-1) is a 76 amino acids protein that is highly chemotactic for monocytes, T lymphocytes, basophils and NK cells during infl ammatory responses. MCP-1 is synthesized by a wide variety of cell types including monocytes and vascular endothelial sand is also known to regulate adhesion molecule expression and cytokine production in monocytes. Over-expression of MCP-1 has been associated with rheumatoid arthritis, atherosclerosis, melanoma, invasive breast cancer and the promotion of angiogenesis.

LIGHT (soluble) (human), detection set [for ELISA application]APO-54N-029-KI01 5 x 96 wells

MCP-1 (rat), EIA kitADI-900-077 1 x 96 wells




Range 0 – 20 ng/ml

Species Reactivity Rat




ILEIILEI has been characterized as a key player in both epithelial to mesenchymal (EMT) transition and late-stage carcinoma progression. EMT is a process that involves changes in gene expression, cy-toskeleton organization, cell adhesion, and extracellular matrix composition. ILEI is overexpressed and/or altered in intracellular localization in multiple human tumors, an event strongly correlated to invasion/EMT, metastasis formation, and survival in human colon and breast cancer.

ILEI (human), detection set [for ELISA application]APO-54N-032-KI01 5 x 96 wells





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OsteoprotegerinOsteoprotegerin (OPG) is a secreted tumor necrosis factor receptor homolog that inhibits osteoclastogenesis and increases bone densityin vivo. OPG binds to RANKL (TRANCE) and with a similar affi nity to TRAIL and contributes determinatively to the bone remodeling as well as to the pathogenetic mechanism of bone malignancies and disorders of mineral metabolism.


Free Osteoprotegerin (human), ELISA kit


20 pg/ml 0.031 – 2 ng/ml APO-54N-042-KI01 1 x 96 wells

Free Osteoprotegerin (human), detection set [for ELISA application]

Human Culture supernatant, plasma and serum

2 pg/ml 0 – 250 pg/ml APO-54N-028-KI01 5 x 96 wells

Progranulin

RANKL

Progranulin (PGRN) has been shown to be a widely expressed pluripotent growth factor which plays a role in processes such as develop-ment, wound repair and infl ammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTD). In addition, PGRN has also been linked to tumorigenesis.

Bone remodeling is tightly regulated by a molecular triad composed of OPG/RANK/RANKL. The receptor activator of NF-κB ligand (RANKL) enhances osteoclastogenesis via interaction with its re-ceptor RANK, whereas osteoprotegerin (OPG) inhibits this osteoclastogenesis by binding to RANKL. An increase in RANKL expression leads to bone resorption and bone loss. Imbalances of the RANKL/OPG system have been related to the pathogenesis of Paget’s disease, benign and malignant bone tumors, postmenopausal osteoporosis, rheumatoid arthritis, bone metastases and hypercalcemia.

RANKL (soluble) (human), ELISA kitAPO-54N-016/1-KI01 1 x 96 wells


Sample Type Plasma and serum

Sensitivity ~1.5 pg/ml



Progranulin (human), ELISA kit Human Culture supernatants, plasma and serum



Progranulin (mouse), ELISA kit Mouse Culture supernatants and serum



y = 3E-05x + 0.216R² = 0.998

0

0.2

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Protein AStaphylococcus aureus Protein A is a 42 kDa cell wall constituent that is characterized by its binding affi nity to the Fc portion of some immunoglobulins, especially the IgG class. Protein A is commonly used for purifying antisera, as immunodetection, and visualization techniques. Protein A participates in a number of protective biological functions including antitumor, toxic and carcinogenic activities, thus necessitating the removal of potential Protein A contaminants from antibody preparations for therapeutic use.

Protein A EIA kitADI-900-057 1 x 96 wells


Sample Type Protein A purified IgG preparations


Range 15.63 – 1,000 pg/ml

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TNF-αActive tumor necrosis factor α (TNF-α), exerts proinfl ammatory signals via binding and inducing trimerization of TNF-receptor 1 (TNF-R1) expressed on most normal and transformed cells, or to TNF-receptor 2 (TNF-R2), expressed on endothelial and most immune cells. TNF signaling regulates hematopoiesis, differentiation, endothelial cell activation, apoptosis, lipid metabolism, tumor progression, and immune surveillance, and dysregulation of TNF or its receptors is implicated in numerous disease states including cancer, osteoporosis, autoimmune disease, diabetes, and atherosclerosis.


TNF-α (human), EIA kit Human Culture supernatants, plasma and serum

8.43 pg/ml 15.63 – 1,000 pg/ml ADI-900-099ADI-901-099ADI-902-099


10 x 96 wellsTNF-α (mouse), EIA kit Mouse Culture supernatants and

serum3.9 pg/ml 31.25 – 2,000 pg/ml ADI-900-047 1 x 96 wells

TNF-α (rat), EIA kit Rat Culture supernatants 12.0 pg/ml 31.3 – 2,000 pg/ml ADI-900-086A 1 x 96 wells

VEGFVEGF (vascular endothelial growth factor) is a potent polypeptide regulator of blood vessel function. VEGF promotes an array of responses in the endothelium, including hyperpermeability, endothelial cell growth, angiogenesis and enhanced glucose transport. Several alternate splice isoforms of VEGF are known. Two high-affi nity receptors for VEGF, VEGFR1 and VEGFR2, have been identifi ed and characterized.

VEGF (human), EIA kitADI-900-080 1 x 96 wells




Range 15.6 – 1,000 pg/ml

TGF-β1Many cells can synthesize transforming growth factor β 1 (TGFβ1; TGFβ; TGFB) which is secreted to function in proliferation, differentiation, transformation, signaling and apoptosis. This cytokine has been implicated in many diseases like diabetes, renal disease, and many cancers including prostate and colon. This protein has generated tremendous excitement as a potential tumor-specifi c cancer therapeutic. As a stable soluble dimer, it selectively induces apoptosis in many transformed cells but not in normal cells.

TGF-β1 EIA kitADI-900-155 1 x 96 wells

Species Reactivity Human, mouse, rat and cow



Range 31.25 – 1,000 pg/ml

TL1ATL1A is a TNF-like cytokine that is expressed predominantly by endothelial cells and monocytes. It binds to the death-domain receptor (DR)3 and provides costimulatory signals to activated lymphocytes. Through this interaction, it promotes cell survival and secretion of proin-fl ammatory cytokines. The TL1A/DR3 pathway plays an important role in Th1-mediated intestinal diseases, such as Crohn’s disease.


TL1A (soluble) (human), ELISA kit Human Culture supernatants and serum

15 pg/ml 39 – 2,500 pg/ml APO-54N-027-KI01 1 x 96 wells

TL1A (soluble) (human), detection set [for ELISA application]

Human Culture supernatants and serum

20 pg/ml 0 – 2,5 ng/ml APO-54N-024-KI01 5 x 96 wells

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CD14The myeloid differentiation antigen CD14, 55 kDa, acts as the major receptor for bacterial lipopolysaccharide (LPS). It cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). The CD14 glycoprotein is expressed on macro-phages and monocytes and involved in the clearance of apoptotic cells.


CD14 (soluble) (human), ELISA kit Human Culture supernatants, plasma and serum

n/a 5 – 50 ng/ml ALX-850-302/1-KI01 1 x 96 wells

CD14 (soluble) (mouse), ELISA kit Mouse Culture supernatants, plasma and serum

n/a 5 – 50 ng/ml ALX-850-303/1-KI01 1 x 96 wells

COX-2

HETE

Cyclooxygenases-1 and 2 (COX-1 and COX-2) are integral membrane proteins which catalyze the fi rst step of prostaglandin, thromboxane and prostacyclin production from arachidonic acid. Inhibi-tion of COX-1 and COX-2 enzymes with nonsteroidal anti-infl ammatory drugs (NSAIDs) including aspirin, ibuprofen and the new COX-2 inhibitors, acutely reduces infl ammation, pain and fever.

HETEs are byproducts generated by the metabolism of arachidonic acid by lipoxygenases. 12(S)-HETE is the stereospecifi c hydroxy prod-uct from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE is chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth muscle cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. 15-Hydroxyeicosatetraenoic acid [15(S)-HETE] is the major hydroxy derivative of arachidonic acid when acted upon by 15-lipoxygenase. It is also the primary monohydroxy acid synthesized by the lipoxygenase activity of cyclooxygenase-1 upon linoleic acid. 15(S)-HETE acts as a paracrine regulator of smooth muscle and lung neutrophil recruitment, and elevated levels of 15(S)-HETE are associated with asthma, rhinitis, chronic paranasal sinusitis and rheumatoid arthritis.

COX-2 (human), EIA kitADI-900-094 1 x 96 wells






12(S)-HETE EIA kit Species independent Culture supernatants, plasma, serum and urine

146 pg/ml 195 – 50,000 pg/ml ADI-900-050ADI-901-050


15(S)-HETE EIA kit Species independent Culture supernatants, plasma, serum and urine

69.21 pg/ml 78.1 – 20,000 pg/ml ADI-900-051ADI-901-051


HODEThe major human dietary poly-unsaturated fatty acid is linoleic acid, an essential fatty acid that can be metabolized to either 13-hydroxyoctadecadienoic acid [13(S)-HODE], 13(R)-HODE or 15(S)-HETE. Changes in the intracellular ratio of 13(S)-HODE to 15(S)-HETE have been linked to a number of physiological conditions including cancer and cardiovascular disease. 13(S)-HODE helps to me-diate cell adhesion and excessive proliferation that is critical in the formation of tumors and metas-tases, as well as the formation of atherogenic plaques.

13(S)-HODE EIA kitADI-900-108 1 x 96 wellsADI-901-108 5 x 96 wells


Sample Type Culture supernatants, cell lysates, serum, saliva, tissue and urine


Range 3.9 – 1,000 ng/ml

INFLAMMATION

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OsteopontinOsteopontin (OPN) is an extracellular matrix cell adhesion protein that is expressed in bone, although it is also expressed in other tissues. The notable presence of osteopontin in a variety of tumors is strongly correlated to pathological stage, suggesting its critical role in tumor invasiveness, progression and metastasis. In addition, osteopontin inhibits inducible nitric oxide synthase activity, thereby protecting tumor cells from NO-mediated macrophage cytotoxic attack.


Osteopontin (human), EIA kit Human Culture supernatants, milk, plasma and urine

0.110 ng/ml 2 – 32 ng/ml ADI-900-142 1 x 96 wells

ImmunoSet™ Osteopontin (human), ELISA development set

Human Culture supernatants, milk and plasma

0.016 ng/ml 0.03 – 1 ng/ml ADI-960-142 10 x 96 wells

Osteopontin (rodent), EIA kit Mouse and rat Culture supernatants, plasma and urine

3.03 ng/ml 3.13 – 100 ng/ml ADI-900-090A 1 x 96 wells

Cysteinyl LeukotrieneCysteinyl leukotriene receptors CysLT1 and CysLT2 are present on mast cells, eosinophil and en-dothelial cells. During cysteinyl leukotriene interaction, they can stimulate proinfl ammatory activities such as endothelial cell adherence and chemokine production by mast cells. They are potent lipid mediators in infl ammation inducing smooth muscle contractions and increased capillary permeabil-ity. In excess, the cysteinyl leukotrienes can induce anaphylactic shock.

Cysteinyl Leukotriene EIA kitADI-900-070 1 x 96 wellsADI-901-070 5 x 96 wells


Sample Type Culture supernatants and urine


Range 78.1 – 2,500 pg/ml

LBPLBP (lipopolysaccharide binding protein) is a 50 kDa polypeptide mainly synthesized in hepatocytes and is released as a 58 to 60 kDa glyco-protein into the bloodstream after glycosylation. LBP is an acute phase glycoprotein and binds to the lipid A portion of lipopolysaccharide (LPS) with high affi nity and catalyzes the CD14 dependent cellular activation by LPS.


LBP (soluble) (mouse), ELISA kit Mouse and rat Culture supernatants, plasma and serum

n/a 1– 50 ng/ml ALX-850-305/1-KI01 1 x 96 wells

LBP (soluble) ELISA kit Human, dog, pig, rab-bit, bovine and horse

Culture supernatants, plasma and serum

n/a 5– 50 ng/ml ALX-850-304/1-KI01 1 x 96 wells

LTB4Leukotrienes are major products of 5-lipoxygenase metabolism of arachidonic acid. Leukotriene B4 (LTB4) stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggrega-tion of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of infl amma-tory diseases and immunoregulation.

LTB4 EIA kit ADI-900-068 1 x 96 wellsADI-901-068 5 x 96 wells


Sample Type Culture supernatants, plasma, saliva and urine


Range 11.7 – 3,000 pg/ml

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PGE1Prostaglandin E1 (PGE1) is synthesized from dihomo-γ-linolenic acid (DGLA). PGE1 has been shown to have a number of biological actions, including vasodilation, proliferation of vascular smooth mus-cle cells, platelet aggregation and has been shown to have insulin-like actions. Its effects are in-duced by receptor mediated elevation of cAMP.

PGE1 EIA kitADI-900-005 1 x 96 wellsADI-901-005 5 x 96 wells


Sample Type Culture supernatants, plasma, serum, saliva and urine


Range 4.88 – 5,000 pg/ml

PGE2Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in infl ammation, cancer, atherosclerosis, autoim-mune disease, and sepsis. PGE2 mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE2 has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDs) or by modulation of its receptors by downregulation or binding antagonists.


PGE2 EIA kit Species independent Culture supernatants, serum, saliva, urine and whole blood

13.4 pg/ml 39.1 – 2,500 pg/ml ADI-900-001ADI-901-001


PGE2 high sensitivity EIA kit Species independent Culture supernatants, serum, saliva, urine and whole blood

8.26 pg/ml 7.8 – 1,000 pg/ml ADI-930-001ADI-931-001


PGE2 CLIA kit Species independent Culture supernatants, serum, saliva, urine and whole blood


PGE2 FPIA kit Species independent Culture supernatants 684 pg/ml 1,562 – 100,000 pg/ml ADI-920-001 100 tests

6-keto-PGF1α6-keto-Prostagladin F1α (6-keto-PGF1α) is a stable hydrolysis product of prostacyclin (PGI2). PGI2 has a short half-life in buffer (2-3 minutes) and the prostacyclin production is typically monitored by measurement of the 6-keto-PGF1α metabolite and its derivative in urine, 2,3-dinor-6-keto-PGF1α. PGI2 has fuctions that include inhibition of vasoconstrictor responses to pressor hormones, platelet anti- and disaggregatory properties, and reproductive functions.

6-keto-PGF1α EIA kitADI-900-004 1 x 96 wellsADI-901-004 5 x 96 wells


Sample Type Culture supernatants, serum, saliva and urine


Range 3.2 – 50,000 pg/ml

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PTX3PTX3, belonging the “long” pentraxins, is a cytokine modulated molecule that is produced in a va-riety of tissues during infl ammation. PTX3 interacts with several ligands, including growth factors, extracellular matrix component and selected pathogens, playing a role in complement activation and facilitating pathogen recognition by phagocytes. In conjunction with the other components of innate immunity, the pentraxins use their pattern-recognition property for the benefi t of the host.

PTX3 (human), detection set [for ELISA application]ALX-850-299-KI01 1 Set

Species Reactivity Human and bovine (weak)

Sample Type Culture supernatants, EDTA plasma or serum (weak)

Sensitivity > 75 pg/ml

Range 75 – 750 pg/ml

15-deoxy-Δ12,14-PGJ215-deoxy-Δ12, 14-Prostaglandin J2 (15-d-PGJ2) is one of the ultimate dehydration products of PGD2, formed via the intermediate Δ12, 14-PGJ2. 15-d-PGJ2 exerts both pro- and anti-infl ammatory effects in a cell-type and concentration-dependent manner, with many of these effects mediated via binding to the gamma isoform of the peroxisome proliferators-activated receptor (PPAR-γ). 15-d-PGJ2 appears to induce HO-1 expression by a PPAR-γ-independent mechanism mediated by the generation of reactive oxygen species (ROS).

15-deoxy-Δ12,14-PGJ2 EIA kitADI-900-023 1 x 96 wellsADI-901-023 5 x 96 wells


Sample Type Culture supernatants, plasma, saliva and urine


Range 195 – 200,000 pg/ml

PGF2αProstaglandin F2α (PGF2α) is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. It is a potent bronchoconstrictor and has been implicated in asthma attacks, and in terminating pregnancies and inducing labor at term.


PGF2α EIA kit Species independent Culture supernatants, plasma, serum, saliva and urine

6.71 pg/ml 3.05 – 50,000 pg/ml ADI-900-069ADI-901-069


PGF2α high sensitivity EIA kit

Species independent Culture supernatants, milk, plasma, serum, saliva and urine

0.98 pg/ml 1.95 – 2,000 pg/ml ADI-930-069ADI-931-069


8-iso-PGF2α EIA kit Species independent Culture supernatants, tissue and urine

16.3 pg/ml 6.1 – 100,000 pg/ml ADI-900-010ADI-901-010


Direct 8-iso-PGF2α EIA kit

Species independent Plasma, serum and tissue 40.0 pg/ml 160 – 100,000 pg/ml ADI-900-091ADI-901-091


9α,11β-PGF2α EIA kit Species independent Culture supernatants, plasma, serum, saliva and urine

12.5 pg/ml 32 – 500,000 pg/ml ADI-900-012ADI-901-012


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Prostacyclin

Thromboxanes

Prostacyclin (PGI2) is involved in platelet aggregation, vasoconstriction, and reproductive functions. PGI2 has a half life of 60 minutes in plasma but only 2 to 3 minutes in buffer. The production of PGI2 is typically monitored by measurement of 6-keto-PGF1α and 2,3-dinor-6-keto-PGF1α. 6-keto-PGF1α is produced by the non-enzymatic hydration of PGI2, and is further metabolized in urine to the 2,3-dinor derivative.

The two major thromboxanes are thromboxane A2 (TXA2) and B2 (TXB2). They belong to the family of lipids known as eicosanoids. TXA2is involved in platelet aggregation, vasoconstriction and reproductive functions, but has a short half-life (37 seconds) under physiological conditions. Thus the in vivo production of TXA2 is measured by monitoring TXB22 and its metabolite 2,3-dinor TXB2. 11-Dehydro-TXB2 is a major metabolite of TXB2 found in urine and plasma.

Urinary Prostacyclin EIA kitADI-900-025 1 x 96 wellsADI-901-025 5 x 96 wells




Range 7.81 – 2,000 pg/ml


TXB2 EIA kit Species independent Culture supernatants, plasma, serum, saliva and urine

10.54 pg/ml 13.7 – 10,000 pg/ml ADI-900-002ADI-901-002


11-dehydro-TXB2 EIA kit Species independent Culture supernatants and urine 4.31 pg/ml 9.8 – 10,000 pg/ml ADI-900-092ADI-901-092


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Natural Products & Antibiotics

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Cylindrospermopsin

Microcystins

Cylindrospermopsin is a cyanobacterial cytotoxin comprising a tricyclic guanidine moiety combined with a hydroxymethyl uracil. It exhibits a completely different mechanism of toxicity than micro-cystins, as it is toxic to liver and kidney tissue and is thought to inhibit protein synthesis and to covalently modify DNA and/or RNA.

Microcystins comprises a group of toxic, cyclic heptapeptides produced by several genera of cyanobacteria. Microcystins are potent inhibi-tors of the serine/threonine protein phosphatases type 1 (PP1) and 2A (PP2A). PP1 and PP2A are two major protein phosphatases in eu-karyotic cells which have been shown to be important in tumor suppression. All structural congeners of microcystin act as hepatotoxins.

Cylindrospermopsin ELISA kitALX-850-332-KI01 1 x 96 wells

Sample Type Water samples



Product Sample Type Sensitivity Range Prod. No. Size

Microcystins (Adda specifi c) ELISA kit Water samples 0.1 ng/ml 0.15 – 5 ng/ml ALX-850-319-KI01 1 x 96 wells

Microcystins-DM ELISA kit Water samples 0.1 ng/ml 0.15 – 5 ng/ml ALX-850-324-KI01 1 x 96 wells

Microcystins coated tube kit Water samples 00.15 ppb 0 – 5 ppb ALX-850-333-KI01 1 Kit

Okadaic Acid

Saxitoxin

Okadaic acid is a naturally occuring polyether toxin produced by marine dinofl agellates. It is a potent and selective inhibitor of protein phosphatases 1 and 2A (PP1 and PP2A). It mimics the effects of insulin, enhances transmitter release at neuromuscular junctions, and causes vasodila-tion. Okadaic acid is an extremely useful tool for studying cellular processes that are regulated by phosphorylation.

Saxitoxin (STX) is a neurotoxin naturally produced by certain species of marine dinofl agellates. It is responsible for the human illness known as paralytic shellfi sh poisoning (PSP). STX, one of the most potent natural toxins known, acts on the voltage-gated sodium channels of nerve cells, preventing normal cellular function and leading to paralysis.

Okadaic acid ELISA kitALX-850-335-KI01 1 x 96 wells

Saxitoxin ELISA kitALX-850-334-KI01 1 x 96 wells

Sample Type Water or shellfish

Sensitivity 0.1 µg/g

Range 0.2 – 5 µg/g

Sample Type Water or other contaminated samples




Obesity & Metabolism

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ANGPTLsSeven proteins have been identifi ed to contain a coiled-coil domain and a fi brinogen-like domain similar to those found in angiopoietins, and have been therefore designated angiopoietin-like proteins (ANGPTLs) 1-7. ANGPTL3 and ANGPTL4 are involved in lipid metabolism. ANGPTL6 suppresses gluconeogenesis through an Akt/FocO1-dependent pathway. In addition, several ANGPTLS have been identifi ed as regulators of angiogenesis.


ANGPTL3 (human), ELISA kit Human Culture supernatants, plasma and serum



ANGPTL3 (mouse/rat), dual ELISA kit

Mouse and rat Culture supernatants, plasma and serum



ANGPTL6 (human), ELISA kit Human Culture supernatants, plasma and serum

1.2 ng/ml 0 – 200 ng/ml AG-45A-0016EK-KI01AG-45A-0016TP-KI01AG-45A-0016PP-KI01


AdiponectinAdiponectin, also known as ACRP30 (30 kDa adipocyte complement-related protein) is a hormone synthesized and secreted by adipocytes. It is involved in controlling the whole-body metabolism, particularly by increasing insulin sensitivity in muscle and liver, and by increasing fatty acid oxidation in muscle. Currently, most investigators are focusing on the potential anti-diabetic, anti-atherogenic, anti-proliferative and anti-infl ammatory activities of adiponectin.


Adiponectin (human), ELISA kit Human Culture supernatants, plasma, serum and tissue



Adiponectin (human), competitive ELISA kit


100 pg/ml 0.001 – 1 µg/ml AG-45A-0002EK-KI01AG-45A-0002TP-KI01AG-45A-0002PP-KI01


Adiponectin (mouse), ELISA kit Mouse Serum 50 pg/ml 0 – 16 ng/ml AG-45A-0004EK-KI01AG-45A-0004TP-KI01AG-45A-0004PP-KI01


Adiponectin (rat), ELISA kit Rat Culture supernatants, plasma and serum



Adiponectin (rhesus monkey, macaque), competitive ELISA kit

Monkey Culture supernatants, plasma and serum

100 pg/ml 0.001 – 1 µg/ml AG-45A-0003EK-KI01 1 x 96 wells

ChemerinChemerin (tazarotene-induced gene 2; TIG2) is the ligand of the ChemR23 receptor and a chemoattractant factor for human immature dendritic cells (DCs), macrophages, and NK cells. In addition, chemerin acts as an adipokine that affects adipogenesis and glucose homeostasis in adipocytes. The serum levels of chemerin in human correlate with BMI, circulating triglycerides, and blood pres-sure. Furthermore, a recent study indicates that chemerin also induces endothelial angiogenesis and MMP production and activity.

Chemerin (human), detection set [for ELISA application]APO-54N-040-KI01 5 x 96 wells





CTRPsCTRP5 and CTRP7 belong to a new highly conserved family of adiponectin paralogs designated as C1q tumor necrosis factor-α-related pro-teins (CTRPs). All CTRPs are secreted glycoproteins when expressed in mammalian cells. Defects in CTRP5 are a cause of late-onset retinal degeneration (LORD). CTRP7 is predominantly expressed in adipose tissue and induces AMP-activated protein kinase phosphorylation.


CTRP5 (human), competitive ELISA kit


1 ng/ml 0 – 5 µg/ml AG-45A-0031EK-KI01AG-45A-0031TP-KI01AG-45A-0031PP-KI01


CTRP7 detection set [for ELISA application]

Human and mouse Culture supernatants and serum


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FTO

Leptin

The Fto (fat mass and obesity associated) gene was fi rst cloned after identifi cation of a fused toe (Ft) mutant mouse, whose phenotype arised from a 1.6mb deletion of six genes, including Fto. Some specifi c FTO gene variations were shown to correlate directly with obesity and even more, with type-II diabetes.

The adipokine leptin is a hormone secreted predominantly by adipose tissue that signals through leptin receptors in the hypothalamus to decrease appetite and increase energy expenditure. In peripheral tissues, leptin antagonizes insulin signaling, increases fatty acid oxidation, decreases insulin production in pancreatic β cells and promotes fertility.


FTO (human) (intracellular), ELISA kit

Human Cell lysates 50 pg/ml 0 – 10 ng/ml AG-45A-0025EK-KI01AG-45A-0025TP-KI01


FTO (mouse) (intracellular), ELISA kit

Mouse Cell lysates 20 pg/ml 0 – 20 ng/ml AG-45A-0028EK-KI01AG-45A-0028TP-KI01



Leptin (human), EIA kit Human Culture supernatants, plasma and serum

23.4 pg/ml 31.3 – 2,000 pg/ml ADI-900-028A 1 x 96 wells

Leptin (mouse), EIA kit Mouse Culture supernatants, plasma and serum

25.4 pg/ml 50 – 3,200 pg/ml ADI-900-019A 1 x 96 wells

Leptin (rat), EIA kit Rat Culture supernatants, plasma and serum

67.2 pg/ml 100 – 6,400 pg/ml ADI-900-015A 1 x 96 wells

Nampt (Visfatin/PBEF)The NAD+ biosynthesis pathway in mammals consists of two steps involving the enzymes NAMPT and Nmnat. NAMPT converts nicotin-amide into nicotinamide mononucleotide (NMN) which then is converted to NAD+ by Nmnat. Two forms of NAMPT exist, an intracellular form (iNAMPT) and an extracellular form (eNAMPT). While the function of iNAMPT as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNAMPT is still a matter of debate. It has been suggested that eNAMPT regulates insulin secretion in β cells, activates pro-infl ammatory signaling and is involved in the induction of inducible nitric oxide synthase (iNOS; iNOS II). In general, NAMPT-mediated NAD+ biosynthesis has widespread actions on cellular senescence, aging, obesity, diabetes, cancer, and infl ammation.


Nampt (Visfatin/PBEF) (human), (intracellular) ELISA kit

Human Cell lysates 30 pg/ml 0 – 32 ng/ml AG-45A-0008EK-KI01AG-45A-0008TP-KI01


Nampt (Visfatin/PBEF) (human), ELISA kit

Human Serum 30 pg/ml 0 – 16 ng/ml AG-45A-0006EK-KI01AG-45A-0006TP-KI01AG-45A-0006PP-KI01


Nampt (Visfatin/PBEF) (mouse/rat), (intracellular) dual ELISA kit

Mouse and rat Cell lysates 50 pg/ml 0 – 64 ng/ml AG-45A-0009EK-KI01AG-45A-0009TP-KI01


Nampt (Visfatin/PBEF) (mouse/rat), dual ELISA kit

Mouse and rat Serum 50 pg/ml 0 – 64 ng/ml AG-45A-0007EK-KI01AG-45A-0007TP-KI01AG-45A-0007PP-KI01


Nesfatin-1Nesfatin-1 is an anorexigenic polypeptide produced by cleavage of the NEFA/nucleobindin-2 (NUCB2). It is expressed in the hypothalamic nuclei in the brain and in several peripheral tissues including the stomach and pancreas. Peripheral as well as intracerebroventricular administration of nesfatin-1 suppresses feeding behavior.

Nesfatin-1 (mouse/rat), detection set [for ELISA application]APO-54N-036-KI01 5 x 96 wells

Species Reactivity Mouse and rat

Sample Type Biological samples



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Omentin 1Omentin 1 (intelectin-1; endothelial lectin HL-1; intestinal lactoferrin receptor) is an adipokine that is preferentially produced by visceral adi-pose tissue. It is secreted from stromal vascular cells but not adipocytes. In vitro studies revealed that omentin 1 enhances insulin-mediated glucose-uptake in isolated adipocytes and activates Akt/PKB signaling.


Omentin 1 (human), ELISA kit Human Culture supernatants, plasma and serum

0.4 ng/ml 0.5 – 32 ng/ml APO-54N-034-KI01 1 x 96 wells

Omentin 1 (human), detection set [for ELISA application]

Human Culture supernatants and serum

0.4 ng/ml 0.78 – 50 ng/ml APO-54N-026-KI01 5 x 96 wells

RBP4

Resistin

Retinol binding protein 4 (RBP4; RBP) is a 21 kDa secreted protein and member of the lipocalin family. It is known as the primary transporter of retinol (vitamin A) to tissues. In addition RBP4 is an adipokine which might be involved in the development of insulin resistance.

Resistin (FIZZ3; ADSF) has a relative mass of 12 kDa and belongs to a family of cysteine-rich C-terminal domain secreted proteins, known as resistin-like molecules (RELMs) or “found in the infl ammatory zone” (FIZZ) proteins. In rodents, increased resistin-levels have been shown to impair insulin action, while genetic ablation or down-regulation of the resistin gene improves insulin sensitivity. In human the role of resistin is still a matter of debate. Only a minority of reports have demonstrated human resistin expression in adipocytes, while most reports identifi ed infl ammatory cells and bone marrow-derived as the main source, indicating a role in infl ammatory responses.


RBP4 (human), ELISA kit Human Culture supernatants, plasma, serum and urine



RBP4 (human), competitive ELISA kit

Human Culture supernatants, plasma, serum and urine

1 ng/ml 0.001 – 5 µg/ml AG-45A-0010EK-KI01AG-45A-0010TP-KI01AG-45A-0010PP-KI01


RBP4 (mouse/rat), dual ELISA kit

Mouse and rat Culture supernatants, plasma, serum and urine




Resistin (human), ELISA kit Human Plasma 100 pg/ml 0 – 16 ng/ml AG-45A-0023EK-KI01AG-45A-0023TP-KI01AG-45A-0023PP-KI01


Resistin (mouse), ELISA kit Mouse Serum 100 pg/ml 0 – 60 ng/ml AG-45A-0024EK-KI01 1 x 96 wells

VaspinVisceral adipose tissue-derived serpin (vaspin) is a member of serine protease inhibitor (SERPIN) family isolated from visceral adipose tissue of Otsuka Long–Evans Tokushima Fatty (OLETF) rats, a model of abdominal obesity and type 2 diabetes. Expression of human vaspin in adipose tissue is regulated in a fat depot-specifi c manner and could be associated with parameters of obesity, insulin resistance, and glucose metabolism.

Vaspin (human), ELISA kit AG-45A-0017EK-KI01 1 x 96 wellsAG-45A-0017TP-KI01 Twin Plex 2 x 96 wellsAG-45A-0017PP-KI01 Penta Plex 5 x 96 wells




Range 0 – 2 ng/ml

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ACE2The renin-angiotensin-aldosterone system (RAAS) regulates systemic blood pressure and renal function and is a key player in renal and cardiovascular disease. Angiotensin-converting enzyme 2 (ACE2) is an enzymatically active homolog of ACE that degrades angiotensin I, angiotensin II, and other peptides. The enzyme is present in the kidney, liver, adipose tissue and pancreas. ACE2 is implicated in cardiovascular and renal (patho)physiology, diabetes, pregnancy, lung disease and serves as a receptor for SARS and NL63 coronaviruses.

ACE2 (human), ELISA kitAG-45A-0022EK-KI01 1 x 96 wellsAG-45A-0022TP-KI01 Twin Plex 2 x 96 wellsAG-45A-0022PP-KI01 Penta Plex 5 x 96 wells





Akt1/2The Akt (PKB) family of protein kinases are serine/threonine kinases, with three mammalian family members identifi ed to date (Akt1, Akt2, Akt3). Akt is a well-characterized member of PI3 kinase-mediated signaling pathways, regulating cell growth, apoptosis, glycogen synthesis, and other cel-lular responses through its phosphorylation of downstream substrates. Deregulation of Akt signaling has been associated with cancer, diabetes, and schizophrenia.

[pSer473]Akt1/2 EIA kitADI-900-162 1 x 96 wells





AldosteroneAldosterone is a steroid hormone synthesized from cholesterol in the adrenal cortex. Aldosterone is metabolized in the kidney and liver, and functions as the key mineralocorticoid in the control of so-dium and potassium balance. Synthesis and release of aldosterone by the adrenal gland is primarily regulated by the renin-angiotensin-aldosterone system (RAAS).

Aldosterone EIA kitADI-900-173 1 x 96 wellsADI-901-173 5 x 96 wells


Sample Type Plasma, serum and urine



β-CateninThe catenins (α, β, and γ) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. Catenin also binds to N-Cadherin and co-immunoprecipitates with APC. Cadherin/Catenin complexes are linked to the cytoskeleton via a direct association between α-actinin and α-catenin. Increased tyrosine phosphorylation can disrupt catenin-cadherin complex-es, infl uencing cellular adhesion.

β-Catenin EIA kitADI-900-135 1 x 96 wells




Range 125 – 8,000 pg/ml

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cAMP

cGMP

Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme adenylyl cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general me-tabolism.

Cyclic GMP (cGMP) is formed by the action of the enzyme guanylyl cyclase on GTP. Some hormones, such as acetylcholine, insulin, and oxytocin, as well as certain other chemicals like serotonin and histamine cause an increase in cGMP levels. Stimulators of guanylyl cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate, and nitric oxide (NO) also stimulate cGMP levels.


cAMP Complete EIA kit

Species independent Cell lysates, culture su-pernatants, serum, saliva and tissue

Assay Buffer: 0.30 pmol/ml (non-acetylated) 0.039 pmol/ml (acetylated) HCl: 0.39 pmol/ml (non-acetylated) 0.037 pmol/ml (acetylated)

0.78 – 200 pmol/ml (non-acetylated); 0.078 – 20 pmol/ml (acetylated)

ADI-900-163ADI-901-163


Direct cAMP EIA kit

Species independent Cell lysates and tissue (non-acetylated) 0.39 pmol/ml; (acetylated) 0.037 pmol/ml


ADI-900-066ADI-901-066


cAMP EIA kit Species independent Culture supernatants, serum and saliva

(non-acetylated) 0.30 pmol/ml; (acetylated) 0.039 pmol/ml

0.78 –- 200 pmol/ml (non-acetylated); 0.078 – 20 pmol/ml (acetylated)

ADI-900-067ADI-901-067



cGMP Complete EIA kit

Species independent Cell lysates, culture supernatants, plasma, serum, saliva, tissue and urine

Assay Buffer : 0.42 pmol/ml (non-acetylated) 0.043 pmol/ml (acetylated) HCl: 0.604 pmol/ml (non-acetylated) 0.059 pmol/ml (acetylated)


ADI-900-164ADI-901-164


Direct cGMP EIA kit Species independent Cell lysates and tissue (non-acetylated) 0.604 pmol/ml; (acetylated) 0.025 pmol/ml

0.8 – 500 pmol/ml (non-acetylated); 0.08 - 50 pmol/ml (acetylated)

ADI-900-014ADI-901-014


cGMP EIA kit Species independent Culture supernatants, plasma, serum, saliva and urine

(non-acetylated) 0.37 pmol/ml; (acetylated) 0.088 pmol/ml

0.16 – 500 pmol/ml (non-acetylated); 0.16 - 100 pmol/ml (acetylated)

ADI-900-013ADI-901-013


Corticosterone

Cortisol

Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to the stimulation of the adrenal cortex by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Corticosterone production has been shown to increase with stress.

Cortisol (hydrocortisone; compound F) is a steroid hormone synthesized from cholesterol. It is the primary glucocorticoid produced and secreted by the adrenal cortex. Cortisol is found in the blood either as free cortisol, or bound to corticosteroid-binding globulin (CBG). Cortisol is known to regulate metabolism, promoting gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Cortisol production is positively correlated with stress, resulting in increased blood pressure, and exerts immunosuppressive effects on the immune system.

Corticosterone EIA kitADI-900-097 1 x 96 wellsADI-901-097 5 x 96 wells


Sample Type Culture supernatants, feces, plasma, serum and saliva


Range 32 – 20,000 pg/ml


Cortisol EIA kit Species independent Culture supernatants, plasma, serum, saliva and urine

56.72 pg/ml 156 – 10,000 pg/ml ADI-900-071ADI-901-071


Cortisol FPIA kit Species independent Culture supernatants 685 pg/ml 781 – 800,000 pg/ml ADI-920-071 100 tests

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DHEADehydroepiandrosterone (DHEA) is a 17-ketosteroid produced by side chain cleavage of 17-hydroxypregnenolone. DHEA is reversibly converted to its sulfate ester (DHEA-S) and andros-tendiol. The human adrenal glands secrete both DHEA and DHEA-S which in turn serve as the precursors for both estrogenic and androgenic steroids. DHEA has been linked to a number of physiological conditions including depression, systemic lupus erythematosus, and rheumatoid arthritis.

DHEA EIA kitADI-900-093 1 x 96 wellsADI-901-093 5 x 96 wells


Sample Type Culture supernatants, plasma, serum saliva and urine


Range 12.21 – 50,000 pg/ml

Dkk-1Dkk-1 (Dickkopf-1) is a secreted inhibitor of the canonical Wnt signaling pathway, which mediates a variety of cellular processes includ-ing the growth and development of osteoblasts. Dkk-1 disrupts the canonical pathway by binding to the LRP5/6 co-receptors, preventing interaction with Wnt and facilitating the degradation of β-catenin. Dkk-1 is overexpressed in multiple myeloma, increasing bone resorption and the appearance of osteolytic lesions characteristic of multiple myeloma.


Dkk-1 (human), EIA kit Human Culture supernatants, plasma and serum 0.98 pg/ml 7.81 – 500 pg/ml ADI-900-151 1 x 96 wells

Dkk-1 (mouse), EIA kit Mouse Culture supernatants, plasma and serum 116.7 pg/ml 125 – 4,000 pg/ml ADI-900-172 1 x 96 wells

Dkk-1 (rat), EIA kit Rat Culture supernatants, plasma and serum 26.1 pg/ml 39.1 – 1,250 pg/ml ADI-900-171 1 x 96 wells

DLL1The Notch signaling pathway is essential for appropriate cell differentiation and cell fate decisions. Delta-like 1 (DLL1) is one of the known ligands of the Notch receptors and plays a role in mediating cell fate decisions during hematopoiesis. It blocks the differentiation of progenitor cells into the B cell lineage while promoting the emergence of a population of cells with the characteristics of a T cell/NK cell precursor. Recently it has been shown that DLL1 is also essential for postnatal arteriogenesis.

DLL1 (soluble) (human), ELISA kitAG-45A-0027EK-KI01 1 x 96 wellsAG-45A-0027TP-KI01 Twin Plex 2 x 96 wellsAG-45A-0027PP-KI01 Penta Plex 5 x 96 wells





EndothelinEndothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue “Big ET” molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ETA and ETB. Activation of ETA increases vasoconstric-tion and sodium retention, thereby increasing blood pressure.


Big Endothelin-1(human), EIA kit

Human Culture supernatants and serum 0.23 pg/ml 0.78 – 100 pg/ml ADI-900-022 1 x 96 wells

Big Endothelin-1 (rat), EIA kit

Rat Culture supernatants, plasma and serum 0.72 pg/ml 0.78 – 100 pg/ml ADI-900-073 1 x 96 wells

Endothelin-1 EIA kit Human, mouse, rat, cow, dog, pig and rabbit

Culture supernatants, plasma and serum 0.40 pg/ml 0.78 – 100 pg/ml ADI-900-020A 1 x 96 wells

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Erk1/2The extracellular regulated kinases, Erk-1 and Erk-2, both play a critical role in cellular proliferation. These extracellular kinases, are known to reside downstream of the mitogen activated protein kinase/extracellular protein kinases referred to as MEK. Both MEK-1 and MEK-2 phosphorylate and activate Erk on the conserved T-E-Y motif within the activation loop of the kinase.


Erk1/2 EIA kit Human, mouse and rat Cell lysates 22.9 pg/ml 156 - 20,000 pg/ml ADI-900-152 1 x 96 wells

[pThr202/Tyr204]Erk1/2 EIA kit Human, mouse and rat Cell lysates 38.3 pg/ml 62.5 - 2,000 pg/ml ADI-900-098 1 x 96 wells

[pThr202/Tyr204]Erk1/2 CLIA kit Human, mouse and rat Cell lysates 34.4 pg/ml 37.5 - 600 pg/ml ADI-910-098 1 x 96 wells

17β-Estradiol

Estriol

Gastrin I

17β-Estradiol (E2) is a female sex steroid hormone synthesized enzymatically from acetate, cholesterol, progesterone and testosterone. In addition to estradiol’s well described anatomic and physiological regulation of reproduction and secondary sex characteristics, it also infl u-ences diverse activities such as bone growth, brain development and maturation, and the intracelluar concentration of calcium and certain second messenger molecules.

Gastrins are a family of sequence-related carboxyamidated peptides produced by endocrine G cells of the antrum mucosa and are potent stimulators of gastric acid secretion. Increased serum gastrin levels are associated with duodenal ulcers, Helicobacter pylori infections, colorectal carcinomas and other tumors and cancerous lesions.

Estriol is an important steroid hormone produced in large quantities during pregnancy. It is produced by the feto-placental unit and accounts for 90% of all estrogens produced during pregnancy.

Estriol EIA kitADI-900-100 1 x 96 wells




Range 122 – 500,000 pg/ml


17β-Estradiol (serum/plasma), EIA kit Species independent Plasma and serum 14.0 pg/ml 15.6 – 1,000 pg/ml ADI-900-174ADI-901-174


17β-Estradiol EIA kit Species independent Culture supernatants and saliva

28.5 pg/ml 29.3 – 30,000 pg/ml ADI-900-008ADI-901-008



Gastrin I (human), EIA kit Human Culture supernatants, plasma and serum 7.27 pg/ml 39.1 – 10,000 pg/ml ADI-900-026ADI-901-026


Gastrin I (rat), EIA kit Rat Culture supernatants, plasma and serum 78.1 pg/ml 78.1 – 5,000 pg/ml ADI-900-149 1 x 96 wells

GSK-3βGlycogen synthase kinase 3β (GSK-3β) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3β on serine 9, which prevents GSK-3β from phosphorylating glycogen synthase. In addition to insulin signaling, GSK-3β participates in the Wnt signaling pathway, where it forms a complex with axin, β-catenin and adenomatous poly-posis coli (APC) protein. Clinically, there is considerable interest in GSK-3β inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer’s disease.


GSK-3β EIA kit Human and rat Cell lysates 74.4 pg/ml 78.1 – 5,000 pg/ml ADI-900-144 1 x 96 wells

[pSer9]GSK-3β EIA kit Human, mouse and rat Cell lysates 9.0 pg/ml 62.5 – 2,000 pg/ml ADI-900-123A 1 x 96 wells

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IGF-1Insulin-like growth factor-1 (IGF-1) is a polypeptide hormone similar in structure to insulin. It is involved in regulation of neuronal growth and development in the central and peripheral nervous system. IGF-1 is a potent inhibitor of apoptosis in neurons and activates the Akt signaling pathway via the IGF-1R and Insulin receptors.

IGF-1 (human), EIA kitADI-900-150 1 x 96 wells


Sample Type Plasma and serum


Range 187 – 6,000 pg/ml

NFκBNuclear factor κB (NFκB) was identifi ed as a sequence specifi c transcriptional activator. NFκB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IκB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-infl ammatory cytokines, and viral infection, IκB is phosphorylated and degraded by the proteosome. The active NFκB heterodimer is translocated into the nucleus and induces gene expression.

NFκB p65 ELISA kit ADI-EKS-446 2 x 96 wells



Sensitivity n/a

Range n/a

Jnk1/2The stress-activated protein kinases α and γ (SAPKα and γ), also know as c-Jun N-terminal kinases 1 and 2 (Jnk1/2) are serine/threonine protein kinase belonging to the MAP kinase family. The ex-tracellular stimuli leading to cell stress include infl ammatory cytokines (via Rho and Rac), growth factors, protein synthesis inhibitors and osmotic stress amongst others. Both Jnk1 and Jnk2 must be dually phosphorylated in the activation loop, at threonine 183 and tyrosine 185, to achieve full enzymatic activity.

[pThr183/Tyr185]Jnk1/2 EIA kitADI-900-106 1 x 96 wells




Range 125 – 4,000 pg/ml

MEK1MEK is a dual specifi city kinase capable of phosphorylating both tyrosine and threonine residues. The MEK family, which is also known as MAP kinase kinase, phosphorylates MAP kinases on the conserved T-X-Y motif. Phosphorylation of MAPK by MEK results in an in-crease in MAPK activity. MEK is involved in a diverse array of cellular processes such as stress-activated response, apoptosis, cytokine-induced cell proliferation, and DNA recombination during meiosis.


[pSer218/Ser222]MEK1 EIA kit Human, mouse and rat Cell lysates 85.2 pg/ml 187.5 – 6,000 pg/ml ADI-900-119 1 x 96 wells

MEK1 EIA kit Human and rat Cell lysates 139.0 pg/ml 312.5 – 10,000 pg/ml ADI-900-122A 1 x 96 wells

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OxytocinOxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hy-pothalamus and stored in the posterior pituitary. Once thought to be limited to female smooth muscle reproductive physiology, more current fi ndings have determined that oxytocin also functions as a neurotransmitter, may be involved in neuropsychiatric disorders, social/sexual behavior, and is important in male reproductive physiology. Oxytocin and the related neurohypophysial peptide, Arg-vasopressin, maintain renal water and sodium balance.

Oxytocin EIA kitADI-900-153 1 x 96 wellsADI-901-153 5 x 96 wells


Sample Type Culture supernatants, milk, plasma and serum


Range 15.6 – 1,000 pg/ml

p38

Progesterone

Six proteins of the p38 subfamily of MAP kinases have been identifi ed to date, including: p38α (SAPK2; CSBP) and its splice variant Mxi; p38β; p38γ (ERK6; SAPK3); and p38δ (SAPK4). p38 MAP kinases contain a conserved T-G-Y motif in their activation loop and are activated in response to cytokines, hormones, GPCRs, and osmotic and heat shock to mediate primarily pro-apoptotic and pro-infl ammatory responses.

Progesterone is the major female sex hormone, and is responsible for reproductive-related activi-ties such as breast glandular development, the endometrial aspects of the menstrual cycle, and the establishment and maintenance of pregnancy. In addition, progesterone also directs pregnancy support physiology including changes in carbohydrate, protein and lipid metabolism, thermoregula-tion, sodium reabsorption in renal tubules and the reduction of alveolar and atrial carbon dioxide partial pressures (pCO2).

[pThr180/Tyr182]p38 EIA kitADI-900-101 1 x 96 wells

Progesterone EIA kitADI-900-011 1 x 96 wellsADI-901-011 5 x 96 wells

Species Reactivity Human and mouse



Range 156 – 5,000 pg/ml


Sample Type Culture supernatants, serum and saliva


Range 15.62 – 500 pg/ml

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SerotoninSerotonin (5-hydroxytryptamine; 5-HT) is a monoamine found in the central nervous system, gas-trointestinal tract, and blood with broad physiological functions in neurotransmission, gastric motil-ity, hemostasis, and cardiovascular integrity. Seven subclasses (5-HT 1-7) of serotonin receptors have been identifi ed, a majority of which are members of the seven transmembrane domain-con-taining GPCR family, and are the target of several anti-depressant drugs.

Serotonin EIA kitADI-900-175 1 x 96 wells


Sample Type Platelets, plasma, serum and urine



Substance PSubstance P is an undecapeptide hormone (RPKPQQFFGLM) that mediates a number of biological activities including nausea, anxiety, pain and vasodilation. It is a member of the tachykinin family of neuropeptides and is widely distributed in the peripheral and central nervous systems of vertebrates.

Substance P EIA kitADI-900-018 1 x 96 wellsADI-901-018 5 x 96 wells




Range 9.76 – 10,000 pg/ml

Testosterone

Vasopressin

Testosterone is one of the most important androgens secreted into the bloodstream and is synthesized from pregnenolone which is itself formed from cholesterol. Testosterone is secreted by the Leydig cells of the testes and effects both primary and secondary sexual develop-ment such as muscle mass and sex drive. In adult humans, testosterone circulates in plasma predominately bound to proteins, including specifi c sex hormone binding globulin (SHBG) and nonspecifi c proteins such as albumin.

Arginine vasopressin (AVP) is a nonapeptide hormone produced in magnocellular neurons of the hypothalamus, which functions as an antidiuretic in the kidney and systemically as a regulator of vasoconstriction, blood volume, and blood pressure. AVP acts by binding to a set of specifi c sev-en transmembrane domain-containing G-protein coupled receptors (GPCR) classifi ed into three groups: V1a (V1), V1b (V3), and V2.

[Arg8]-Vasopressin EIA kit ADI-900-017 1 x 96 wellsADI-901-017 5 x 96 wells


Sample Type Culture supernatants


Range 4.10 – 1,000 pg/ml


Testosterone EIA kit Species independent Culture supernatants, plasma, serum and saliva

5.67 pg/ml 7.81 – 2,000 pg/ml ADI-900-065ADI-901-065


Testosterone (human), EIA kit Human Plasma, serum and urine

2.6 pg/ml 3.9 – 1000 pg/ml ADI-900-176ADI-901-176


42


Alphabetical Index

ELIS

A/EI

A Ki

t

ELIS

ADe

velo

pmen

t Set

Page

ACE2 • 35

Adiponectin • 31

ADMA • 14

Akt1/2 • 35

Aldosterone • 35

ANGPTLs • 31

BAFF • • 17

Bax • 7

Bcl-2 • 7

cAMP • 36

β-Catenin • 35

CD14 • 23

cGMP • 36

Chemerin • 31

Clusterin • 9

Complement • 17

Corticosterone • 36

Cortisol • 36

COX-2 • 23

αB-Crystallin • 11

CTRPs • 31

Cylindrospermopsin • 29

Cytochrome c • 7

DHEA • 37

Dkk-1 • 37

DLL1 • 37

DNA Damage • 11

Endothelin • 37

Erk1/2 • 38

17β-Estradiol • 38

Estriol • 38

FTO • 32

Gastrin I • 38

GPX3 • 14

GRO/CINC-1 • 17

Grp75 • 12

Grp94 • 12

GSK-3β • 38

HETE • 23

HO-1 • • 14

ELIS

A/EI

A Ki

t

ELIS

ADe

velo

pmen

t Set

Page

HODE • 23

HSP25 • 12

HSP27 • • 12

HSP40 • 13

HSP60 • 13

HSP70 • 13

HSP90 • 13

IFN-γ • 17

IGF-1 • 39

IgG • 17

IgM • 18

IL-1β • 18

IL-2 • 18

IL-4 • 18

IL-6 • 19

IL-8 • 19

IL-10 • 19

IL-33 • 19

ILEI • 20

Jnk1/2 • 39

LBP • 24

Leptin • 32

Cysteinyl Leukotriene • 24

LTB4 • 24

LIGHT • 20

M30 CytoDeath™ ELISA Kit • 7

M30-Apoptosense® ELISA Kit • 8

M65® ELISA Kit • 8

MCP-1 • 20

MDA5 • 11

MEK1 • 39

Microcystins • 29

Myeloperoxidase • 15

Nampt (Visfatin/PBEF) • 32

Nesfatin-1 • 32

Netrin-4 • 8

NFκB • 39

Okadaic Acid • 29

Omentin 1 • • 33

Osteopontin • • 24

ELIS

A/EI

A Ki

t

ELIS

ADe

velo

pmen

t Set

Page

Osteoprotegerin • • 21

Oxytocin • 40

p21 • 10

p27/Kip1 10

p38 • 40

p53 • • 10

PDI • 15

PGE1 • 25

PGE2 • 25

6-keto-PGF1α • 25

PGF2α • 26

15-deoxy-Δ12,14-PGJ2 • 26

Pin1 • 10

Progesterone • 40

Progranulin • 21

Prostacyclin • 26

Proteasome • 15

Protein A • 21

Protein Carbonyl ELISA Kit • 15

PTX3 • 26

RANKL • 21

RBP4 • 33

Resistin • 33

RIG-I • • 11

Saxitoxin • 29

SDMA • 14

Serotonin • 41

Smac/DIABLO • 8

Substance P • 41

Survivin • 9

Testosterone • 41

TGF-β1 • 22

Thromboxanes • 27

TL1A • • 22

TNF-α • 22

Vasopressin • 41

Vaspin • 33

VEGF • 22

XIAP • 9

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