S P E C I F I C A T I O N S

Integrated Solutionscell::explorer HTS pro

Application ConfigurationMultimodule Ultra-High Throughput Screening (uHTS) Platform

Customer Request

A multimodule system that can accommodate both uHTS and HCS in one, integrated platform.

Configuration Requirements

• Fully integrated screening platform to support:

– 96, 384, 384 low volume and 1536-well plates

– nLtohighμLliquidhandling

– Detection instruments for HCS – image based readouts

– Cellular and biochemical multimode read-outs

• Parallel assay processing and offline use of individual instruments

• Parallel and offline access to both workstation modules

Today’sacademicresearchersrequiretoolsandtechnologiesthat can support a broad range of current and future applicationsincludingdifferentmicroplateformats,liquidhandling volumes and detection-based read-out methods; cell::explorer HTS pro was designed to fulfill those diverse needs. The configuration was created with four (4) individual workstations, each able to run independent processes, linked via a bi-directional con veyor belt to create a large integrated screening system poised to automate the entire screening process. Through two (2) integrated 500-plate capacity stores, the compound module has access to nearly 600,000 compounds stored in 384-well plates. Compounds can be transferred into assay plate either in nL amounts utilizingaLabcyteECHOacousticdispenserorinμLamounts through a JANUS® Automated Workstation fitted with both 96- and 384-well pipetting heads. Precision dispensing of reagent and cells is realized through a Flexdrop® IV and two Thermo MultiDrop® Combi dispenser spread over the indi vidual modules. A 198-plate incubator, as well as 40-plate incubator enable plate incubation at different environmental condi tions. Assay results are detected either on an EnVision® Multilabel Plate Reader or on an Opera® confocal microplate imaging reader.

3D view of multimodule uHTS platform

FlexDrop® IV High precision reagent & cell dispenser

Conveyor beltPlate distribution conveyor dispenser

Assay plates Compound platesExample Assay Flow

Image plate

Compound store

Compound store

Incubator 1

Add reagent 1

Add reagent 2Read barcode

Incubator

Incubator

JANUS compoundaddition

Wash plate

Wash

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Top view of multimodule HTS and HCS platform

Cytomat® 6001 IncubatorIncubation

Echo®

nl compound transfer

Washer

MultiDrop® CombiReagent dispenser

500 DC storeCompound plate storage

1000 DC storeCompound plate storage

MultiDrop® CombiReagent dispenser

2

5

78

6

6

8

2

8

103

3

9

4

4

4

1

1316

16

16

12

12

16

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PerkinElmer Components

3rd Party Components

EnVision®

Multilabel Plate Reader

plate::handler™ 6-axis robotPlate handling robot

plate::handler™ 6-axis robotPlate handling robot

TurntablePlate handling device

Opera®

Confocal microplate imaging reader

JANUS® Automated WorkstationMINI Varispan®

with 4 tips

plate::handler™ 6-axis robotPlate handling robot

Lid handling unitLid removal, storage

and replacement

JANUS® Automated WorkstationMINI MDT for 96/384 tips

VSpin centrifugePlate centrifugation

View of system within a research facility