Bucher Biotec AG Viaduktstrasse 42 4051 Basel Phone 061 269 1111 www.bucher.ch

June

‘11

ContentsCell Biosciences Inc. FluorChem® M

Next-Generation Platform for Multiplex Western Blot Detection

Cell Biosciences, Inc., a provider of innovative protein analysis tools to life science researchers, recen-ty announced the launch of the latest addition to their premier line of next-generation imaging prod-ucts, the FluorChem® M MultiFluor system.

This new platform combines high-ly-sensitive quantitative detection of multicolor fluorescent Western blots with key ease-of-use features aimed at simplifying the labora-tory imaging process. MultiFluor Western Blotting kits, a new line of reagents optimized for the Fluor-Chem M, are launching jointly — creating a combined offering that provides a complete solution for multicolor fluorescent imaging.

Multiplex capability enables sci-entists to obtain more information in a single Western blot than che-miluminescence imaging or film, while also providing greater dy-namic and linear range. Multiple proteins can be detected simulta-neously and proteins with co-mi-gration patterns are revealed im-mediately. Additionally, the need to strip and reprobe blots for sub-sequent detection of other proteins is eliminated, which minimizes pro-tein loss and results in shorter assay times and higher data integrity.

FluorChem M features intuitive software, touchscreen operation and pre-optimized protocols that let researchers start imaging right out of the box — no training required. Using a unique browser-based interface, scientists can control and monitor image ac-quisition and access data re-motely from any networked com-puter or mobile WiFi device such as Apple iPhone or iPad. Chemi-luminescent, colorimetric and UV-excited gels and blots can also be imaged on the system.

Get a free Apple iPad® with ev-ery system purchase (offer valid through 30.8.2011).

Interested in a system Demo at your lab? Simply give us a call!

Next-Generation Platform for Multiplex Western Blot Detection

New representation: Discover True High Through-

put Flow Cytometry

A Fluorescent Human Co-Culture Angiogenesis Model

Ground Breaking New Dis-pensing Technology

Flexible and Modular Laser Capture Microdissection

High Throughput Automat-ed Patch Clamp System with Plate Reader Simplicity and Temperature Control

New BioFlux 1000Z: Experi-ence the Power of Flow

Discover why over 1200 Scientists are using the XF Analyzer for a Faster, Bet-ter and more Accurate Measurement of Real-Time Cellular Bioenergetics

DropSense96 – Your “Nano-Drop” for automated UV/VIS-Quantification

The Benefits of Combining Fluorescence and Atomic Force Microscopy

New Tools Enable Total As-say Assembly on a Single Instrument

Upcoming Events

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June 2011

Key Features 96 wells in < 3 min.

384 wells in <12 min.

No priming & No dead vol-ume

Analyze up to 5’000 cells/second

HyperCytPRO intelligent soft-ware for data management

Detects bright and dim cells in each well

Simple set up – User friendly

Brings cytometry into screen-ing labs

Simply call us (061 269 1111) for a system demonstration in your lab!

Bucher Biotec AG is very pleased to announce the appointment as the exclusive Swiss Distributor for the product portfolio of IntelliCyt™ Corporation.

IntelliCyt™ develops innovative high throughput cell screening solutions using flow cytometry technology for drug discovery and life science research.

The company offers innovative products that make it possible for researchers to handle large-scale cell-based assays faster than previously possible.

The New Benchtop Screening Solution for Suspension Cells

The HTFC™ Screening Sys-tem is a revolutionary new instrument platform de-signed to perform high-throughput, multiplexed, single-cell analysis on cells or particles in suspension.

Alternatively, HyperCyt Sam-pler connects to your flow cytometer inlet, and using the HyperCyt sample han-dling technology, transfers samples in a rapid sequen-tial fashion.

The speed to succeed: screen up to 40 wells per minute in 96well or 384well plates & support of bullet tubes and deep well plates.

The IntelliCyt MultiMetric Apoptosis Assay simultaneously provides 5 endpoints that can aid in the determination of apoptosis progression by incorporating traditional “early” and “late” stage markers, as well as potential mechanistic information on activating events

Look Insidewith the IntelliCyt MultiMetric Apoptosis Kit

HTFC™ Screening System

No sample goes to waste, run your screen with limited sample supply and save costs

Powerful data analysis and management tools de-signed for your multi-user environment.

Optional flow cytometer-specific automation soft-ware is available so that the HyperCyt can control your flow cytometer and collect the data files automatically.

HyperCyt technology is the most cost-effective way, to rap-idly and efficiently screen com-pound or antibody libraries us-ing flow cytometry.

Introducing the IntelliCyt HTFC™ Screening System

Discover True High Throughput Flow Cytometry

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June 2011

The ArcturusXT™ system is a unique microdissection instru-ment that combines Laser Cap-ture Microdissection (LCM) and Ultraviolet (UV) Laser Cutting in one platform.

The open, modular design of the ArcturusXT system provides un-paralleled research flexibility and versatility. The system removes the guesswork from the microdis-section process by allowing re-searchers to maintain custody of the sample throughout the experi-ment, ensuring that only the de-sired material has been collected.

The gentle red laser capture technique preserves biomolecu-lar integrity and is ideal for single cells and small numbers of cells, whereas UV laser is well-suited

Applied Biosystems® ArcturusXT™ Microdissection System

Flexible and Modular Laser Capture Microdissection

Fluxion Biosciences IonFlux™ System

High Throughput Automated Patch Clamp System with Plate Reader Simplicity and Temperature Control

for microdissecting dense tissue structures and for capturing large numbers of cells.

The ArcturusXT LCM system is vali-dated by over 750 peer reviewed publications of more than 100 journals worldwide, and over 1,000 platform installations. Laser microdissection of individual cells is flanked by validated reagent kits, including tissue staining, RNA isolation & amplification as well as support of formalin-fixed & par-affin embedded tissues.

The Arcturus platform was re-cently acquired by Life Technolo-gies, however, Bucher Biotec AG remains your contact for Switzer-land being the Swiss representa-tion for Arcturus LCM systems and reagent kits.

The IonFlux System delivers a high throughput electrophysiol-ogy solution for ligand-gated and voltage-gated ion channels with “plate reader” simplicity.

The system’s unique microfluidic well plate format eliminates pi-petting steps, allows continuous recording of cell ensembles, and rapidly delivers compounds. The System delivers outstanding patch clamp performance in a com-plete, easy-to-use system.

Designed for higher throughput screening, the system provides 64 integrated amplifiers with a throughput capability of 1,000 data points per hour.

Parallel-cell ensemble recording improves reliability and minimizes variability. The system’s unique microfluidic design automates all aspects of the compound appli-cation and recording protocol. No additional pipetting or washing steps are required. Voltage clamp-

ing occurs continuously through-out the entire experiment. This optimized design offers significant advantages in throughput and speed of compound application.

Please contact us in order to re-ceive the latest IonFlux Applica-tion note, discussing temperature as a critical parameter for patch clamp data. Screening against hERG liability at physiological tem-perature yields data that is likely to provide a better correlation to cardiac risk in vivo.

The IonFlux system is unique among automated patch clamp (APC) systems in providing a mod-ule for temperature control, a valuable feature for hERG safety screening and other temperature dependent ion channel targets.

In order to discuss your specific requirements simply give us a call (061 269 1111).

In order to receive further details, simply send us an email or check our website (www.bucher.ch) to obtain detailed information or customer provided video article via JoVE.

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June 2011

Seahorse Bioscience XF Extracellular Flux Analyzer

Discover why over 1200 Scientists are using the XF Analyzer for a Faster, Better and more Accurate Measurement of Real-Time Cellular Bioenergetics

The XF Extracellular Flux assays al-low non-invasive and label-free measurements of cellular bioener-getics. Simultaneous recording of O2 consumption and H+ produc-tion in a fully integrated system us-ing 24-well or 96-well cell culture plates allows researchers easy and efficient access to the physiologi-cal state of primary cells, cell-lines, islets or isolated mitochondria.

By measuring the two major en-ergy producing pathways of the cell simultaneously, mitochon-drial respiration and glycolysis, scientists get the most physiologi-cally relevant bioenergetic as-say available, resulting in a bet-ter overall view of metabolism. The XF Analyzer addresses basal oxygen consumption, fatty acid

Cellular Bioenergetics Webinars On-Demand:Ready when you need them.

To View On-Demand Webinars, visit www.seahorsebio.com

Paolo BernardiUniversity of Padua

Mitochondrial Bioenergetics in Disease: Toward Mitochondrial Therapy

Martin BrandBuck Institute &MRC Dunn

Rate & Coupling Efficiencyof Oxidative Phosphorylationin Mitochondria & Cells

Craig BeesonMedical University of South Carolina

How Bioenergitics Drive Biological Function

Orian ShirihaiTufts School of Medicine

How to Measure Mitochondrial Function in Whole Islets

Antonio C. BiancoUniversity of Miami

Thyroid Hormone Deiodination: a Key Switch for Energy Expenditure

Gary L. WrightEast Tennessee State University

Protective Bioenergetic Responses of Cardiomyocytes to Hypoxic Stress

Vamsi MoothaHarvard Medical School

Human Disorders of Bioenergetics

Russell H. SwerdlowUniversity of Kansas School of Medicine

Cybrid Modeling of Sporadic Neurodegenerative Disorders

Yvonne WillPfizer, Inc.

Mitochondrial Toxicity: Designing Screens to Reduce NCE Attrition

James DykensPfizer, Inc.

Bioenergetic & Metabolic Profiling in Drug Discovery

John J. LeMastersMedical University of South Carolina

Mitochondrial Function in the Life & Death of Cells

David G. NichollsBuck Institute

Quantifying the Mitochondrial ProtonCircuit in Intact Cells

Brian PolsterUniversity of Maryland School of Medicine

Assessing Mitochondrial Dysfunction & the Role of Calpain Proteases in Vitro

Victor Darley-UsmarUniversity of Alabama

Response of the Mitochondria to Oxidative Stress in Cardiovascular Cells

Ben Van HoutenUniversity of Pittsburgh Cancer Institute

Oxidative Stress, Mitochondrial DNADamage & Disease

Metabolic Diseases, Obesity, & Diabetes

Mitochondrial Dysfunction & Toxicity

Neurodegeneration, Aging, & Oxidative Stress

oxidation and metabolism of glu-cose and amino acids for kinetic metabolic information.

We want to welcome you to the popular monthly Seahorse Biosci-ence Cellular Bioenergetics We-binar Series featuring key thought leaders discussing their areas of expertise and the Seahorse XF technology.

Please see www.seahorsebio.com which lists all of the upcoming and past webinars, also sorted by Re-search Area.

Seahorse Bioscience now allows studying mitochondrial function and dysfunction in living cells with less setup and clean up than tra-ditional techniques, such as Clark electrodes.

Fluxion Biosciences Inc BioFlux 1000Z System

New BioFlux 1000Z: Experience the Power of FlowThe new BioFlux 1000Z System is a fully-integrated high content screening platform for bridging the gap between in vitro and in vivo experiments. It integrates

the industry-leading Zeiss Axio-Observer microscopy worksta-tion with the BioFlux electro-pneumatic pumping system for controlling shear flow.

The BioFlux Montage software offers full hardware control over all motorized components and is the command center for design-ing flow experiments, acquiring images and analyzing data.

One System ... many applications

Oncology: Cell adhesion and rolling, Transmigration, Migra-tion and Invasion, Chemot-axis

Platelet Function: Platelet aggregation and adhesion, Thrombosis, Atherosclerosis models

Vascullar Biology: Special-ized Cell Culture, Cell ad-hesion, Atherosclerosis, Mechanical loading , Throm-bosis, Migration and Invasion

Immunology: Transmigration, Migration, Cell Adhesion and Rolling, Wound Healing

Stem Cells: Controlled Dif-ferentiation, Growth Condi-tions, Mechanical Loading, Bioproduction

Microbiology: Biofilm Growth, Mutant Screens, Antibacte-rial or antifungal Screening, Host-Pathogen Interactions, Adhesion Strength

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June 2011

The Trinean DropSense96® is a new multichannel spectropho-tometer for quick and precise UV-VIS spectral analysis of micro-liter droplets of DNA/RNA, protein or small compounds.

The spectrophotometer has all the characteristics of a plate reader and can be used with standard 96-well Microplates and specific DropPlate16/96 chips with 1.5 -3 µl sample volume.

The unique features of the DropSense96 are

UV-VIS absorption spectrometry

Full spectrum analysis of 96 samples in less than 5 minutes (230 – 750 nm)

Integrated barcode scanner for sample tracking

Compatible with both Drop-Plate16/96 and classic 96well microplates

Predefined protocols for DNA, RNA and protein concentra-tion measurements

User-friendly software on a re-mote PC

Trinean DropSense96® Multichannel Spectrophotometer

DropSense96 – Your “NanoDrop” for automated UV/VIS-Quantification

Key applications:

Nucleic acids concentration measurements

Purity calculations

Measurement of dye labe-ling efficiency of micro-array samples

Direct protein quantifications

Color measurements (e.g. Bradford)

General UV/VIS measurements

The unique features of the Drop-Plate16 and 96 are:

1,5-3,0 µl samples

16 to 96 sample disposable technology

Compatible with liquid han-dling robots

No sample evaporation with-in 2 hours

Dynamic range: 5-3500 ng/µl dsDNA

No sample dilution needed

Trinean DropSense96® UV-VIS Spectrophotometer

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June 2011

Angiogenesis, which is the forma-tion of new blood vessels from pre-existing vessels, is mandatory for tumor growth. Angiogenesis is also involved in other pathologi-cal disorders such as psoriasis and macular degeneration.

The IncuCyte FLR is the latest im-aging system from Essen Biosci-ence and is designed to enable time-lapse imaging of live-cell fluorescent reporters. The Incu-CyteFLR fits inside a cell culture incubator allowing researchers to gather fluorescent and phase contrast time-lapse images around-the-clock.

The Preddator is a unique, highly versatile precision instru-ment, designed specifically for successful dispensing of low volumes of cells, beads, sur-factants, oils and biochemical reagents into 96- to 3,456-well plates and other labware.

Low volume pipetting (20 nl - 100 μl) is used routinely in drug discovery and research applications. How-ever, current technologies are inadequate at high throughput, causing problems such as block-

Essen Bioscience Inc. GFP-AngioKit™

A Fluorescent Human Co-Culture Angiogenesis Model

Redd & Whyte’s Preddator S1 Liquid Handler

Ground Breaking New Dispensing Technology

The system is ideal for monitoring processes, which require hours to days such as angiogenesis.

Essen’s CellPlayer™ GFP-Angio-Kit, an in vitro co-culture assay system, recapitulates all stages of the angiogenic cascade, and allows the study of pro- and anti-angiogenic responses. The kinetic method used here is in favor to currently available meth-ods, in that it provides dynamic information not available from end point experiments.

One of the most difficult aspects of an angiogenesis assay is mea-suring the metrics associated with angiogenic events. Essen Bioscience Angiogenesis Analy-sis Module quantitates multiple metrics, including tube length, tube area and branch point

formation, over the course of each experiment, and has been optimized for providing a kinetic readout. Furthermore, all metrics can be validated by the stored images and movies.

Combining GFP-AngioKits with In-cuCyte FLR enables kinetic and quantitative measurements of both the stimulation and inhi-bition of microvascular tubule formation. This unique combina-tion shows how the GFP-AngioKit, in conjunction with IncuCyte FLR, can be used to screen for drugs affecting all stages of angiogen-esis and to dissect signal trans-duction pathways.

Interested in a system Demo at your lab? Simply give us a call (061 269 1111) or send us an email (info@bucher.ch)!

age of tips, foaming of reagents containing surfactants, or dam-age to cells.

Preddator’s new technology over-comes these issues to ensure highly accurate and reproducible dispensing of complex materials into all types of microtitre plates, including those with peripheral evaporation wells and channels. The system can be programmed to dispense any pattern and is ideal for a broad range of appli-cations, including MALDI spotting.

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June 2011

Integration of optical and atomic force microscopy (AFM) provides a powerful tool to obtain comprehensive infor-mation on a variety of samples. Especially combining fluores-cence microscopy and the AFM technique provides com-plementary information: the fluorescence about the loca-tion of labelled molecules not detectable by transmission light microscopy, the AFM finally on the topology of the sample.

The design of the JPK Nano-Wizard® 3 AFM allows its integra-tion into inverted optical devices providing different optical tech-niques like epifluorescence. The use of the DirectOverlay™ fea-ture available for the JPK SPM software enables real optical integration, not only by detect-ing the position of the cantilever within the optical image but also by correcting optical distortions caused by optical lenses.

In this report the combination of sensitive fluorescence detec-tion and AFM, and the way they

JPK Instruments NanoWizard 3 BioAFM

The Benefits of Combining Fluorescence and Atomic Force Microscopy

are complementing each other are described by different ap-plications.

Imaging Cellular structures and extracellular matrix proteins

MC3T3 osteablasts (fig. 1) were stained for f-actin using FITC-phalloidin. The cells were grown on coverslips for use with the CoverslipHolder™. By applying phalloidin staining the actin stress fibers could be visualized using fluorescence microscopy. Additional topographical in-formation could be derived by AFM imaging revealing the fine structure of the filaments.

Another example where the AFM image reveals substruc-tures invisible by fluorescence imaging is D-periodic collagen. Collagen type-1 forms thick fi-bers (approx. 30 nm) if polym-erized on glass (Cisneros et al. 2006, J. Struct. Biol. 154: 232-245). The D-banding pattern can be nicely resolved using AFM imaging (fig. 2).

Fig. 1:Fluorescence image of MC3T3 fibroblasts stained for filamentous actin with inserted de-flection image. Cells were stained with FITC-phalloidin. The AFM image was taken in contact mode in liquid. The scan region was 15 µm x 15 µm, the height range around 300 nm.

Fig. 2: Right: Fluorescence Image of labelled collagen type 1 on glass and AFM image (box).Left: AFM Image of the collagen fibers displaying the D-banding. The scan region was 2 µm x 2 µm, the height range around 50 nm.

Conclusion

The DirectOverlay™ feature al-lows for real optical integra-tion and provides optical and topographic information with-in one region. Fluorescence la-belled cellular components like the cytoskeleton and traffick-ing molecules can be optically tagged and the morphology of the corresponding region char-acterized by AFM imaging. When investigating structures in the nanometer range that can-not be resolved optically, fluo-rescence can help to roughly detect the structures and thus serve as an orientation tool for the search for interesting re-gions to be finally resolved by AFM imaging.

In order to discuss your specific AFM requirements contact us ei-ther by email (info@bucher.ch) or by using the attached info re-quest card.

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June 2011

FEMS 2011 Palexpo Geneva

June 26 - 30, 2011

HUPO 2011 Human Proteome Organization Palexpo Geneva

September 4 - 7, 2011

Euro AFM Forum 2011 ETH Zürich

September 7 - 9, 2011

MipTec 2011 The Leading Event for Drug Discovery Congress Center Basel

September 20 - 22, 2011

1st. Intl. SystemsX.ch Conference on Systems Biology

Congress Center Basel October 24 - 26, 2011

Upcoming Events

Labcyte Inc. Echo® Acoustic Liquid Handling Systems

New Tools Enable Total Assay Assembly on a Single Instrument

Labcyte the world leader in mov-ing liquids with sound has de-veloped new acoustic droplet ejection parameters that for the first time enable users to eject multiple fluid types on a single instrument.

These fluids include common bi-ological buffers, protein and nu-cleic acid solutions, cell culture media, surfactants and DMSO.

These new calibrations open up new application areas such as:

siRNA screening

assembling sub-microliter Biochemical

Cell-based assays

384- and 1536-well PCR

All Echo systems come pre-calibrated so that you can be productive right away. Switch fluid types instantly with a sin-gle mouse-click; no further ad-justment is required in order to achieve the same superior preci-sion and accuracy that our users can expect from all Echo liquid handlers.

These powerful new capabilities are available in bundled pack-ages to provide exactly the tools you need for your applications.

Echo Screening Package

Echo 520, 550 or 555 liquid handler

Pre-calibrated for 70-100% DMSO

Pre-calibrated for 384LDV and our NEW 1536LDV source plates

Your choice of Echo Plate Re-format or Echo Cherry Pick software

Echo Omics Package

Echo 550 or 555 liquid handler

Pre-calibrated for most com-monly used biological solutions

Pre-calibrated for 384LDV and 384PP source plates

Your choice of Echo Plate Re-format or Echo Cherry Pick software

In order to find out how Labcyte’s liquid handling systems can im-prove your applications, simply give us a call (061 269 1111).