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8/07/15 1 1 LABORATORY SAFETY TRAINING FOR RESEARCHERS March,2015 Prepared by Dr. Alina Jaworska-Sobiesiak, Chemical Control Centre 2 In this training… Introduction General lab safety rules and prudent laboratory practices in the research laboratory Overview of the chemical, biological and radiation hazards: handling, storage, disposal Special topics Emergency preparedness-how to prepare for them, what to do when they happen Summary - additional resources Final Quiz 3 CCC Laboratory & Research Safety Programs Laboratory & Research Safety Chemicals & lab safety Biological Safety Radiation Safety X-ray Safety Laser Safety Nanomaterials Safety Waste Management Laboratory Safety Checklist for Students 4 1. Introduction

CCC Laboratory & Research Safety Programs 1. Introduction

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8/07/15

1

1

LABORATORY SAFETY TRAININGFOR RESEARCHERS

March,2015

Prepared by Dr. Alina Jaworska-Sobiesiak, Chemical Control Centre

22

In this training… Introduction General lab safety rules and prudent laboratory

practices in the research laboratory Overview of the chemical, biological and

radiation hazards: handling, storage, disposal Special topics Emergency preparedness-how to prepare for

them, what to do when they happen Summary - additional resources Final Quiz

33

CCC Laboratory & Research Safety Programs

Laboratory & Research

Safety

Chemicals & lab safety

Biological Safety

Radiation Safety

X-ray Safety

Laser Safety

Nanomaterials Safety

Waste Management

Laboratory Safety

Checklist for Students

4

1. Introduction

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55

Why is lab training important?

66

UCLA Lab Worker DeathWhy You Need Lab Safety Training

YouTube video:Link1 Link2

77

Key Learning Points from UCLA Accident -Researcher

• The lack of a lab coat – ( the single most significant factor in the severity of the burns that led to Sangji's death)

• She used plastic syringe, Sigma-Aldrich recommends using glass syringe for handling t-BuLi

• She was not prepared for a potential mishap, did no use the safety shower

• She was working alone• She had very little experience with the procedure and

she was making three times as much of the material as she had the previous time

88

Key Learning Points from UCLA Accident - Supervisor

• Training on handling hazardous chemicals and minimizing risk of exposure

• Record-keeping programsWhile Sangji was trained in general lab safety as well as the specific procedure she was performing, the training was not documented.

• Failure to ensure the use of personal protective equipment (PPE)

• Lack of SOP or procedures for the handling of pyrophorics such as the t-butyl lithium

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99

Pyrophoric Liquid Safety

UCLA video 11 min.

https://www.youtube.com/watch?v=21iC4YEgOAs

1010

Objectives

By the end of the session each participant will be able to: Understand of legal framework and that you are

the person responsible for your safety Recognize laboratory hazards Identify strategies of safe laboratory research

work Know about available resources

1111

Responsibilities and Legal Framework

You

University of Windsor Health & Safety Policy

Laboratory: Supervisor, Department, Faculty

GovernmentFederal, Provincial, Municipal

1212

Stakeholders in Your Research Activities

Good Research / Happy Collaborators

University of WindsorFaculty

Deans, Chairs Teaching Staff

Research Colleagues

Principal Investigator

Federal / Provincial Government

Administration

Office of Research Safety Research Committees

Health and Safety

Campus Operations

Custodial Staff Maintenance

Chemical Control Centre

City of Windsor

Waste Operations Emergency Response Services

Grant Agencies

CIHR, NSERC

Researchers

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1313

Governmental Research Safety Regulations

Occupational Health and Safety Act -1979 WHMIS -1988

Waste Disposal & Treatment – reg. 347 Transportation/shipping of hazardous materials -

Transportation of Dangerous Goods (TDG) Biosafety, radiation regulations, others

14

2.Laboratory Safety Rules and Prudent Laboratory Practices

1515

Laboratory Safety Manualhttp://www1.uwindsor.ca/chemicalcontrol/

Know safety rules and follow them Laboratory attires and

personal protective equipment (PPE)

Work practices/handling Emergency

1616

Working Alone / After Hours

• Never work alone in high risk area• The PI is responsible for determining whether the work

requires special precautions, such as having two people in the same room for a particular operations.

• Use buddy system (to check in on you periodically)• Phone the Campus Police (ext.1234) to let them know that

you are working alone;

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Section Summary Your personal laboratory safety depends mostly on YOU There are many laboratory safety rules you must adhere to, the

information and instruction provided cannot be consider all-inclusive

Good judgement and common sense are needed for safety The goal is to minimize the risk of injury or illness by ensuring

that you have: training, Information and guidelines support and equipment needed to work safely

18

3.Small Group Activity

Objective: Learning laboratory safety rules

1919

Outline

Students will be put into groups of 4-5; total of 3 groups

Each member of the group will get a picture Students will be required to identify and write

lab safety rules broken in the situation shown in the picture (time: 3 min.)

Reporting results – discussion (time:~10 min).

20

Activity#1Laboratory

• The photo shows several unsafe lab practices;• Identify and list lab safety rules which apply to the unsafe practices in this picture; (list as

many rules as you can find).

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21

Activity #2 Dispensing Liquid Nitrogen

• What is wrong with this picture?

22

Activity # 3 Handling Acids

• Explain why you should never do this with any acid; describe lab safety rules for this situation.

2323

What is a hazard? What is a risk?

24

What is a hazard? What is a risk?

Hazards are dangers intrinsic to a substance or operation;(anything that can cause harm, e.g. a chemical, electricity, radiation, ladder,)

Risk - the probability of injury associated with working with a substance or carrying out a particular laboratory operation; (i.e. the chance that someone will be harmed by the hazard)For a given chemical, risk can be reduced; hazard cannot.

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Hazard Control

2626

Key Learning Points from UCLA Accident

• The fundamental lab safety controls all fell short in this instance: – administrative (rules and policies),– engineering equipment (providing the right tools), – work practices – personal protective equipment

• The case emphasizes “the importance of developing not just a good training program, but a culture where people actually pay attention to safety – not just, ‘Yeah, I got my annual training and I’m done,’ and that’s it,”

2727

Hierarchy of Hazard Control

1.Eliminate the hazard

2. Substitute with a lesser hazard

3. Install engineering controls

4. Put administrative controls in place

5. Use personal protective equipment (PPE)

Hierarchy of controls (in order of preference)

28

PPE in the LabStandard PPE Not Acceptable

Keep long hair pulled back in a ponytail

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29

Before work…Understand the properties of materials and agents and their prescribed safety procedures: Review MSDS, consult

published resources and/or contact the CCC.

Review applicable Standard Operating Procedures (SOPs)

Ask questions and/or discuss with the PI/Supervisor (potential hazards, equipment, the procedure )

Be familiar: with operating procedures for

ALL equipment used emergency spill procedures,

use of adsorbents and disinfectants

designated escape routes (know alternate)

location of fire extinguishers, eye wash, shower, first aid, and spill kit

3030

Laboratory Experiment Risk Assessment

List materials and methods

Identify hazards

Decide safety measures

Prepare for mishap

Reassess the risk

3131

Key Points for Laboratory Safety Perform science/research safely Watch out for yourself and everyone else in the lab Anticipate problems and prevent them Be careful and aware of your surroundings

The bottom line is:safety is a state of mind, and is about recognizing HAZARD and eliminating or minimizing RISKS as much as possible.

32

4. Overview of the Chemical, Biological and Radiation Hazards

Chemical hazards Biological hazards Radiation hazard- ionizing radiation

- X-ray

Lasers safety

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33

Hazardous Chemicals

Definition: A hazardous chemical is defined as any chemical that is a health hazard or a physical hazard.

3434

3535

Handling Hazardous Chemicals

Know the hazards, know the risks of the particular chemicals in use

Minimize the exposure with engineering controls and administrative procedures; (SOP)

Use eye, face, hand and body protection Special oversight needed for carcinogens Special procedures to follow for handling

cyanides and hydrofluoric acid

3636

Acceptable Transportation

• Use freight elevators for moving chemicals and biological materials between floors. Passenger elevators must not be used for this purpose.

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3737

Storage

3838

Chemical Storage Separate incompatible chemicals. Store based on hazard

class/compatibility do not store alphabetically; check the MSDS or review literature

Separate storage areas for: Flammable and combustible organic liquids

and solvents Acids Dry poisons, salts, and oxidizers Bases

Chemical inventories are a regulatory requirement

3939

What is wrong with this picture?

40

Indicate the appropriate storage for the following materials from the given choices:

1. Sulfuric acid 2. Carbon dioxide 3. Propane 4. Acetone, 4 liters 5. Potassium chloride 6. 30% Hydrogen peroxide

a. Shelf of the lab benchb. Chained to wall,(flammable

gas area)c. Refrigeratord. Corrosive storage cabinet e. Flammable storage cabinetf. Chained to wall, (adjacent to

oxygen)

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41

Indicate the appropriate storage for the following materials from the given choices:

1) Sulfuric acid, 2) Carbon dioxide 3) Propane 4) Acetone, 4 liters 5) Potassium chloride 6) 30% Hydrogen peroxide

a. Shelf of the lab benchb. Chained to wall, (flammable

gas area)c. Refrigeratord. Corrosive storage cabinet e. Flammable storage cabinetf. Chained to wall (adjacent to

oxygen)

Correct answer: 1d……2f…..3b…..4e…..5a…..6c

42

Chemical Waste

Fill out the waste label (full chemical name) attached to the container with the percentage of each component

43

Chemical Waste To have your waste removed you must request a

waste pick-up; email to [email protected]. Original labels are OK for old and outdated materials

(PI’s name, lab location) Each waste stream must be collected in a separate

container Refer to the CCC web site and the Hazardous

Materials Disposal Guide, and the Laboratory Safety Bulletin for more information.

44

Chemical Waste

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4545

Common Waste Streams

4646

Special Waste Sreams

4747

Incompatible Waste

Ethanol Nitric acid Waste container

4848

Fume Hood - How It Works to Protect You

• https://www.youtube.com/watch?v=nlAaEpWQdwA

• https://www.youtube.com/watch?v=nlAaEpWQdwA

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4949

Fume Hood Inspection

• If hood airflow monitor is not a part of your fume hood, tape a Kimwipe to the bottom of the sash and observe if the paper is pulled inward

• Required airflow:80-120 FPM

• Maintenance is conducted by the Facility Services and an external contractor.

5050

Fume Hood Inspection Stickers

Fume hood training: www.uwindsor.ca/ccc

5151

Glove Box

Pressure Check: MUST be checked every day

52

Biohazardous MaterialBiological hazards (biohazards) are biological agents

that can cause disease in humans, plants, animals

Harmful microorganisms (pathogens) are biohazardsBacteria, Fungi, Viruses, Parasites, (Toxins)

Blood, body fluids, cell lines, recombinant DNA, animals

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53

RG1• User risk: low• Community risk:

low

RG2• User risk:

moderate• Community risk:

low

RG3• User risk: high• Community risk:

low/moderate

RG4• User risk: high• Community risk:

high

4 ways to get exposed to biohazards? Know the risk! Biohazards are classified in to 4 risk groups:

Biosafety

The UWindsor currently has RG 1 and 2 biological materials.

54

Control the risk! 4 classifications:

Biosafety

University of Windsor, most labs in Canada

55

Biosafety Containment Level 2 = Risk Group 2

5656

Handling – Working in a CL2 Lab An approved biosafety certificate for all biological

research; (BSL 1 and BSL 2)

You must be aware of potential hazards as per Biosafety Manual and lab specific SOP; training is mandatory

Administrative controls: biohazard signs on door, refrigerator and biohazardous waste etc.

Standard and special practices Use safety containers for transporting containers of

hazardous or infectious materials outside of labs. Note: blood work is performed at CL2 at UWindsor

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5757

Biological Safety Cabinets (BSC)

Class II - primary containment device for work with biohazardous materials; protects: Personnel Product Environment Not designed for chemical use

5858

BSC vs Fumehood Can you use a BSC as a fumehood (ie to ventilate volatile chemicals?)

Can you use a fumehood as a BC? (ie to handle biological agents?)

5959

Biological Waste Laboratories must segregate biological waste

from other wastes

Containers provided by the CCC (yellow plastic bag and paper box)

Certain biological materials must be treated (usually by autoclaving) before entering the waste stream; You are responsible for autoclaving. (Autoclave training is required!)

Place the green label Treated Biological Materials before disposal in dumpsters

6060

Biological Waste Animal carcasses must be disposed of by incineration or other

methods. Contact the CCC for pick-up and disposal.

Sharps: puncture proof containers must be used (available from the CCC; pink), do not overfill.

Before disposal, sharps should not be: re-sheathed, broken, bent or otherwise manipulated

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Biosafety - the Bottom Line Work carefully and diligently with and around biological

agents, to avoid: Infecting yourself, others or the environment Contaminating your research

62

Radiation

What is Radiation?Energy travelling as waves or particles

Types of radiation: Ionizing Non-ionizing

63

Types of Radiation

6464

Why is radiation harmful?

Damage to cells and DNA causes mutations and cancer

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6565

Is radiation harmful?• Where does most ionizing

radiation come from?

• Can you become radioactive from being exposed?

• What is the annual radiation dose limit for the Public? Researchers?

6666

Why is radiation harmful?

6767

Why is radiation harmful?Estimated Risk of Dying (per 100 individuals):• 1 mSv…….……………………………….…0.005• 10 mSv….……………...........................….0.05• 50 mSv………………………………………0.25• 100 mSv…………………………………….0.5-------------------------------------------------------------------------• Motor Vehicle Accident……………………1.9• Cancer………………………………………21.2• Heart Disease………………………….......29• Maple Leafs winning the Stanley Cup……?

Ger

ber e

t al.

Circ

ulat

ion

2009

6868

Radiation Safety Program UWindsor Radiation Safety Program is documented in

the Radiation Safety Manual. Can be found online. Must be familiar with program if working with radioactive

materials – Government regulators refer to it when inspecting facilities.

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6969

Working in a Lab with Radioactive Materials

Training is mandatory if you will be handling RAM (online)

Only the RSO is authorized to order radioactive material

All radioactive materials must be secured to prevent theft or loss

Lost or theft must be reported to RSO All ionizing radiation areas MUST BE posted with

warning signs Use caution when entering and working in a

radiation area Follow standard lab rules

7070

Radiation Protection

Time: minimize the time that you are in contact with radioactive material

Distance: keep your distance. If you double the distance the exposure rate drops by factor of 4

Shielding: Lead, water, or concrete for gamma & X-ray, thick plastic for betas

7171

Radiation Monitoring Common type:

Thermoluminescence Detectors (TLD) used at UWindsor.

Used for x-ray and gamma rays, alpha and beta particles.

7272

Radioactive Waste Collect in appropriate radioactive waste containers and place in a

designated area; (behind Plexiglas shield) Appropriately labelled the waste Contact the HazMat Tech when ready to dispose of material

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7373

X-ray Safety What is an X-Ray? Vs. other radiation sources? X-rays: safe when they are used properly (medical tests); can

be detrimental to human health when used improperly. The University X-Ray Safety Program (www.uwindsor.ca/ccc)

ensures compliance with regulations and facilitates research activity

X-ray warning signs MUST be posted. All X-ray rooms are restricted areas Personal dosimeter badges must be worn

7474

X-ray Crystallography Instrumentat UWindsor

7575

Laser Safety What is a laser?

Emits electromagnetic radiation: UV,VIS, IR

How do accidents occur? Refractive beams The human eyes are the primary organs of

concern. Skin to the lesser degree

7676

Class 3b and 4 lasers fall under the Laser Safety Program

Class 3b - From 5 mW to 500 mW -can easily cause eye injury from intrabeam or specular reflection viewing.

Class 4 - Above 500 mW - can cause eye injury from viewing diffuse reflections, skin injuries and ignite combustible materials in the beam path.

Laser Safety training along with site-specific hands-on training is mandatory

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7777

Laser Safety Wear safety goggles!

NEVER, EVER LOOK INTO ANY LASER BEAM!

For more information on laser safety refer to the standard ANSI Z136

Laser warning light

78

5. Special Topics Equipment Safety Eye wash and Safety Showers Handling Cryogens Handling Gas Cylinders NMR Safety Nanomaterials Safety

7979

Equipment

Do not use equipment unless you are trained and approved as a user.

Be aware about procedures before operating any high voltage equipment.

Equipment failure – broken equipment should be reported to your supervisor. Do not try to fix the problem if you have not been trained to do so.

No jewelry or metal objects around electricity Use 3-prong grounded extension cords; do

not use extension cords for permanent wiring

8080

What not to do

Do not bundle cords while plugged in

Do not strain cords

Properly use plugs

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8181

Eyewash and Safety Showers Know the location Know how to use them Regular testing is

required

Check the eyewash weekly, and the shower monthly

8282

Cryogenic Liquids Precautions Wear insulated gloves and safety

glasses; minimize exposed skin with a proper lab clothing

Fill containers to indicated max. level Perform operations (pouring) slowly in

well ventilated area Transport the cryogens from one floor

to another using a freight elevator in the Essex Hall and without any people inside the cab.

8383

Handling Compressed Gases

MYTHBUSTER.wmv

Proper transport of a gas cylinder

Training online: www.uwindsor.ca/ccc

When no longer in use or during transport : shut valves, relieve gas in regulator, remove the regulator and cap.

Never transport with the regulator in place!

8484

What happens when the valve breaks?

MYTHBUSTER.wmv

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8585

NMR Safety Main hazards: high magnetic fields, handling of cryogens high electrical voltages/RF sources

Spend no longer than necessary within the 5-Gauss zone

Keep metallic items at least 2-3 metres away from the magnet.

Person with medical implants (cardiac pacemakers, biostimulators etc.) MUST remain outside the NMR labs.

8686

NMR Safety

Be aware about the lab ventilation ( the He and N2 gas continuously exhausted by the instrument - install oxygen sensor alarm in the NMR lab)

Handle NMR tubes carefully and reserve them for NMR use only Report any accident to the NMR technician immediately

8787

Nanomaterials:Contain nanoparticles; often have unique optical, electronic, or mechanical properties. Hazard identification is still

difficult (no physical andchemical characteristics)

The potential adverse healtheffects are from inhalation,ingestion or dermal absorption

Precautionary approach shouldbe taken to the risk management

SiC nanopowder is nano-sized SiC super fine powder. Nano-size means nano-meter, which equals 10-9 meters (parts per billion meters = parts per million millimeters)

8888

Nanomaterials – Exposure Controls

• Follow: Nanotoolkit Working Safely with Engineered Nanomaterials in Academic Research Settings

http://www.ehs.ucr.edu/laboratory/nanotoolkit.pdf

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89

Before work…Understand the properties of materials and agents and their prescribed safety procedures: Review MSDS, consult

published resources and/or contact the CCC.

Review applicable Standard Operating Procedures (SOPs)

Ask questions and/or discuss with the PI/Supervisor (potential hazards, equipment, the procedure )

Be familiar: with operating procedures for

ALL equipment used emergency spill procedures,

use of adsorbents and disinfectants

designated escape routes (know alternate)

location of fire extinguishers, eye wash, shower, first aid, and spill kit

9090

Laboratory Experiment Risk Assessment

List materials and methods

Identify hazards

Decide safety measures

Prepare for mishap

Reassess the risk

91

6. Small Group Activity

Objective: Recognition of chemical hazards andunderstanding how to prevent the potentialexposure based on MSDS.

9292

Outline Students will be divided into 4 groups;4-5 students per

group. Each group will obtain MSDS for a hazardous chemical. Each group is required to use the MSDS and answer 5

questions: “Questions to answer - before any work “. Questions will appear on slide.

Total time ? min.

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9393

Questions to answer1. What is the material?2. What are the hazards?3. What are the worst things that could happen?

(probability and severity)4. What are the prudent practices, protective

facilities/equipment, and PPE needed to minimize the risk?

5. Any special conditions/precautions to handle the chemical?

9494

Laboratory Experiment Risk Assessment

List materials and methods

Identify hazards

Decide safety measures

Prepare for mishap

Reassess the risk

95

7. Emergency

UWindsor Emergency Procedures Spills Accident Reporting

9696

Emergencies can happenUniversity - wide emergency response information can be found at: http://www1.uwindsor.ca/campuspolice/system/files/EmergencyGuide.pdf If emergencies occur contact Emergency Medical Services at

extension 911 Know where emergency phone numbers are posted Know where to go and what to do in an emergency

UWindsor Alert App

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9797

Emergencies can happen Each lab and department should have specific written

emergency response procedures, eg:– Spills of hazardous materials – Injury or accident requiring medical attention– Equipment failure resulting in a hazardous situation

• Chemical Control Centre: Spill Response

9898

Small SpillsClean spills only if they are minor and you have training and protective equipment. Warn others and secure the area. Put on/wear PPE before attempting to clean or contain a spill. Use absorbent materials from the Spill Kit to contain or soak

up liquids; if biological use an effective disinfectant. Use appropriate container to collect and dispose of absorbed

hazardous materials.

9999

Large Spills Do not attempt to clean up spill if the spill is large (>4-5

Litres) or too hazardous to be cleaned up without specialequipment - contact Campus Police and/or CCC;

If you are unsure how to clean up a spill or it is too large foryou to handle on your own contact the Chemical ControlCentre at 519-253-3000 ext.3519 immediately for assistance.

For after hour spills please call Campus Policeat 519-253-3000 ext. 911.

Spills must be reported to the CCC using aSpill Incident Report form within 24 hours

For Chemical Spills online presentation & Spill Response Manual see: www.uwindsor.ca/ccc

100100

Accident Reporting After incident or accident, the Supervisor

along with the employee shall complete the Accident/Incident Form, which is found on the Health & Safety Website:www.windsor.ca/safety

Human Resources, Health and Safety will investigate the accident.

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101

8. Summary

Available Resources PI/Supervisor Health and Safety Safety Committees Chemical Control Centre/CCC

Key Points Handouts overview Quiz

102102

Available Resources-PI/Supervisor

Manages all matters of health and safety within his/her labs

Makes sure that the Laboratory Safety Orientation Checklist has been completed

Provides lab safety orientation in the area of: General safety Special or unusual hazards in the lab Use of lab specific equipment and emergency

response

103103

Available Resources –Health and Safety

Manages the University's overall health and safety program. Develops and implement policies and procedures to meet all

of the requirements, set by the Occupational Health & SafetyAct and its applicable regulations.

If a lab is not in compliance with the safe operatingprocedures as outlined in the Manual, Health &Safety has theauthority to close the lab until violations are corrected.Approval of the Dean is not required.

Health and SafetyWebsite: www.uwindsor.ca/safePhone: 519.253.3000.ext.4547

104104

Resources-Special ConsiderationsUniversity policy for pregnant women in laboratory settings:Women who are pregnant are encouraged to discuss workassignments with their supervisor and to seek alternate workassignments if the potential for exposure to teratogens and embryotoxins exist. For assistance contact:Student Disability ServicesP: 519.253.3000.ext.3288E: [email protected]/disability Resources:http://www.cdc.gov/niosh/99-104.htmlhttp://www.cdc.gov/niosh/malrepro.htmlhttp://sciencecareers.sciencemag.org/career_development/previous_issues/ look for April 7, 2006

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105105

EmbryotoxinsEmbryotoxins are substances that cause adverse effects on the developing fetus in pregnant women. Benzene Heavy Metals Carbon Tetrachloride Chloroform Azo dyes Propylene glycol Xylene Formaldehyde Nitrous oxide Toluene

106106

Available Resources - Safety Committees UWindsor

VP-RESEARCH

Research Ethics Board

Human Ethics

Animal Care Committee

Animal Safety

Research Safety

Committee

Laboratory Safety

Biological Safety

Radiation Safety

Laser Safety

Safety Committees ensure that health and safety concerns at UWindsor are identified and addressed.

107

Available Resources –Chemical Control Centre (CCC)

CCC areas of focus: Hazardous materials

Acquisition and waste management

Laboratory safety programs – provide trainings, resources and information on safety

CCC web site: www.uwindsor.ca/ccc

108108

Chemicals AcquisitionPlace the order: In person: Essex Hall B-37 P: (519) 253-3000 ext.3523 F: (519) 973-7013 Email: [email protected]

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109109

Chemicals Acquisition• You may also use the electronic Purchase Order

Requisition (.pdf) document, website: www1.uwindsor.ca/ccc

• Using Hazardous Materials Information System (HMIS) Remote Order Entry System (ROES) but only for previously ordered items

110110

Regulated Research MaterialsRegulated Research Materials

Animals used for research Biohazardous materials

Chemical weapons & precursor chemicals Controlled Goods

Controlled Substances Cyanides

Insects not native to Canada or imported Lasers (Class 3B, 4)

Plants not native to Canada or imported Radioactive materials

Toxic substances Designated substances

Veterinary controlled products X-ray equipment

111111

Key Points to Remember

Use all available resource-you are not alone Get safety training for all hazards to your lab:

chemical, biological, radiological etc. Use Laboratory Experiment Risk Assessment

Process DEVELOP AN ATTITUDE OF SAFETY

AWARENESS and follow the five steps

112112

Laboratory Experiment Risk Assessment

List materials and methods

Identify hazards

Decide safety measures

Prepare for mishap

Reassess the risk

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113113

Questions

114114

Handouts overview

Manuals Laboratory Safety Bulletins (LSB) Useful websites

115115

List of UWindsor Manuals

www.uwindsor.ca/ccc1. Laboratory Safety Manual2. Radiation Safety Manual3. Biological Safety Manual4. Spill Response Manual5. Hydrogen Fluoride Utilization Guidelines6. Hazardous Materials Disposal Guide

116116

Laboratory Safety Bulletins (LSB)

http://www1.uwindsor.ca/chemicalcontrol/

1.LSB: Obtaining the Material Safety Data Sheets2.LSB: Peroxide in the Laboratory3.LSB: Fighting a Class D Fire4.LSB: Use of Workplace Labels at the University of Windsor5.LSB: Hydrogen Fluoride Training Program6.LSB: General Glassware Safety Tips7.LSB: Information on Glove Boxes8.LSB: Compressed Gas Cylinder Safety9.LSB: Handling and Transportation of Cryogenic Liquids10.LSB: Safe Disposal of Empty Chemical Containers11.LSB: Disposal of Sharps

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117117

Laboratory Safety Bulletins

1. LSB: Radioisotope Awareness Training2. LSB: Contamination Monitoring3. LSB: Guidelines for Laser Classification4. University of Windsor’s Radiation Safety Policy

Statement on Laser Pointers

118

119 120120

Useful Websites

Gloves selection website:                           A glove selection and assessment chart done by the gloves manufacturer Ansell can be found at: 

http://www.ansellpro.com/specware/guide.asp

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121121

Useful Websites

Chemical Storage: http://ehs.research.uiowa.edu/chemical-

storage-nine-compatible-storage-group-system

https://extranet.fhcrc.org/EN/sections/ehs/hamm/chap3/section9/index.html

122122

Useful Websites

Working in BSC:

http://ehs.yale.edu/training/proper-use-biological-safety-cabinet-video

123123

Quiz