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lnstitute
Water
Catalogue of Services
Consume/s Guide for SamPling of
Water Served by Street Vendors, Shops & Establishments
l.Thisguidelinesistohelpyouarrangefortestingofwaterservedbyhotel,restaurants,street corner eaterres, ;i;;'k, it'it f itt shopis and establishments who serve water
to their client as u purt oi if.,.ii ,rri.r. ln this guide we will refer to all of them as
vendor[s).{';#'leEll rishts vis-a-vis these.service Pl:-Yi*::^:'^:'iflY"'-:i31',T*"[XYP]J:;;H#;i Pi"i;:# ili: ,n il,, ih; I u b I i c i nte restl
]l',1 ll:::'i:':,a nd ed u c ati o n
;rt;;;it;,;n*r ro ot#i[ini poirntritv of warer served by such vendors.
3. Your sampling strategy will depend on :
i.Whetheryoucanplanapurchaseandusetheoccasiontocollectawatersample,orii,HavemadeaSpotdecisiontosendasampleofthewaterservedbythevendorfortesting.
^ il'T;"ff :1; ;ffi ;;; I ;; il;; il J; ;'11 Y:f i :: Tt'i; I 3l?li T I q:"::]i'.fl i:['Ji:L"ty,J,Y, il; HolXffi ffi ;;; ti.e
'u'u *iL vou to the Y:ld?:;]t^':"pi:1":]fi:oorlelrorrr trre riruurdturv
lr.r,"rt'. iiit;i;; i;;'ily' who can witness the samplego with another Person l
collection Process.5 D; ;;i ;dn the bottle until immediately before sample collection'
6. Do not rinse the bottle.
TPurchasefoodorsuchotherservicefromthevenclorthatisaccompaniedbyseryiceof' ;;rki;; ;;ter. lf the u*Jot iotgttt to serve water' ask for it specifically'
g" Ask foithe bill and settle payment to the vendor and obtain receipt'
s. O;.. ihl bottle gently and transfer lhe water served to you in to it'
ro.l[hly.i"te the"bottll. Wipe any spillage on the.outside of the bottle'
ir.wiit.,rn ple collection oaie. ano vendor indentiflcation using a marker pen
12.Filt in the water surpr."ilorG.iion Record (wcRll and rhe Test Reqursition Letter
IRLt). These ior*, uiJpi*ii.O *itf, rhe lt-is lab prepared water collection bottles'
ih.v Jrn ,jro rr* ao*ntoiJeo iro, tn. IHS web sire http://www.ihs.org.in/wqt
13.The WCR1 trr, ,.qrirullit 'ig;'t"u of an witness' You may ask thevendor or their
representative to ,.t * *itnt'i it tf't vendor' refuses to do so' your friend or family
member can serue u, *ii.uss. ii" mention, whether vou did inform the vendor about
the sampling, and the vendor's response to your request t0 stand as witness'
14.1n case of street ,*noor, *ittrort a iermanrni address, try t0 ascertain the place where
the street u.noo|^ ,.riii,,;#;it;; he park the pus'h cart or,suc-h glher vending
olatform, if any and .;;;il td eiact location in the street where you purchased
service from the vendor.
15'DeliverthesampleatthelaboratoryaSsoonaSpossible,preferablywithinsixhours,and'" ;;ii;[tii;;i taier tnan 24 hours fiom the time of collection'
16'Keepawayfromsunlight'Avoidexposuretoexcessiveheat'Keepinrefrigeratorif'a.lr!.d Oufiu.,y it rntiiipittA-At*uyt keep the bottle in upright p0sition'
17 lf you decide on tne spoi to have the water served by a vendor sent for testing.
i. you may try to qrlcrif get a sample collection bottle from the laboratory. These bottles are
available from the rudrionroffce rouncl the clock, Then follow the above guideltnes'
ii.Else,arrangetotransferthewatertothemostcleanbottleeasilyaccessibletoyou.Forexample'you could empty water" irom a pa.t ageo water bottle and use the same to coliect the sample
to be tested. Anotherllternative wouid be to buy a new plastic or glass bottle and use it'
iii. Collect identifying information about the vendor
iv.CarrytheWatertothelHsandtransferthesametoalabpreparedwatercollectionbottle.v, Fill in the Sample Collection Recorcis, TeSt Requisition Letter give the sample for testing.
vi. water sample collection service is available at the IHS Front off ice round the clock Samples received
during laboratory rrurin.r, t,or,, are processed in next available batch, samples received after
businesshoursareStoredinarefrigeratorandprocessedinthefirstbatchofthenextbusinessday.
T
ume/s Guide for Sampling of
iteet Vendors, Shops & Establishments
arrange for testing of water served by hotel, restaurants,rk, fruit juice shops and establishments who serve watereir service. ln this guide we will refer to all of them as
rse service providers is usually enforceable through thert from the public interest, the information and educationne potability of water served by such vendors.
rpend on :
rase and use the occasion to collect a water sample, or
send a sample of the water served by the vendor for testing.
r collection of a water sample, obtain a sample collectioncarry the same with you to the vendor. lt is preferable to
h as a friend or family, who can witness the sample
nmediately before sample collection.
ervice from the vendor that is accompanied by service oforgets t0 serue water, ask for it specifically.
rent to the vendor and obtain receipt.
rnsfer the water served to you in to it.any spillage on the outside of the bottle.
and vendor indentification using a marker pen.
lection Record (WCRI) and the Test Requisition Letter
ided with the IHS lab prepared water collection bottles.from the IHS web site http://www.ihs.org.in/wqt
signature of an witness. You may ask the vendor or their:ss. lf the vendor, refuses to do so, your friend or family;. Do mention, whether you did inform the vendor about
's response to your request to stand as witness.
)ut a permanent address, try to ascertain the place whererere does he park the push cart or such other vendingd the exact location in the street where you purchased
ratory as s00n as possible, preferably within six hours, and
urs from the time of collection.
roid exposure to excessive heat. Keep in refrigerator ifl. Always keep the bottle in upright position.
ave the water served by a vendor sent for testing.
sample collection bottle from the laboratory. These bottles are
)ffice round the clock. Then follow the above guidelines.
rter to the most clean bottle easily accessible to you. For example,
packaged water bottle and use the same to collect the sample
ve would be to buy a new plastic or glass bottle and use it.
about the vendorj transfer the same to a lab prepared water collection bottle.
lecords, Test Requisition Letter give the sample for testing.
is available at the IHS Front office round the clock. Samples received
rrs are processed in next available batch. Samples received after
efrigerator and processed in the first batch of the next business day.
Water Quality Testing Services
SvCd Service
8125 Water Collection Bottle - 125 ml
B01L Water Collection Bottle - 1000 ml
BPT Basic Potability Test
CPT Complete Polability Test
BCT BacteriologicalAnalysis
BIT Bottled Water lntegrity Test
FLD Fluoride Analysis oi Water
GQT Ground Water Quality Tesl
GBT Ground Waler Bacteriological
Contamination Test
GPT Ground Waler Potability Test.
CAT Chlorine Availability Test
AQT Alum Quality Test
LAT Liquid Chlorine Acceplability Test
SCS Sample Collection Service
FCS Fast Sample Collection Service
ECS Emergency Sample Collection Service
SNS Sanitary Survey of Source
Quantity of
sample
required
Bottle or
Packet
Preparations
NA
NA
Lab B01 L
Lab B01 L
Lab 81 25
NA
Client
Client
Lab-B1 25
Lab-801 L
Client
Client
Client
AA
AA
AA
AA
Page
No.
500 ml
1000 ml
100 ml
Pack
100 ml
500 ml
100 ml
1000 ml
1009 /ml
1 009
100 ml
AA
AA
AA
AA
Z
2
3
4
E
6
6
7
8
9
10
10
11
12
12
12
12
Notes:
1. For tests where lab prepared bottles are to be used for collection of sample, the
laboratory will supply the boltles if test service charges are paid in full.
2. Alternatively, you can collect the laboratory prepared bottles on payment of the deposit
amounl. ln such a case the deposil amount will be adjusted towards test service
charges at the time of reciept of sample, and test requisilion.
3. Where client prepared bottles are adequate, you should follow instructions
contained in this catalogue to prepare the botlle or packet for sample collection.You may, however, purchase laboratory prepared bottles for this purpose. Please, however,
note that the test service charges have been fixed assuming that you will prepare your
bottle / packet for collection of sample. Hence only 50% of bottle deposit will be adjusted
in case you use any of the laboratory prepared bottles,
4. For service charges please refer the lalest price list.
Legend:SvCd: Service Code; NA: Not Applicable; AD: Adjustable Deposit; AA: As Appropriate. Lab : Bottle
prepared by the Laboratory or its equivalent to be used; Client: Bottle Prepared by the client in
accordance with bottle preparation instructions in the catalogue. CB: Estimate on case basis.
tE Water Quality Testing Laboratory, The lHS, HACA Bhavan. Hyderabad, AP - 500 004, lndia. 1
Bottles' 125 ml (8125), and 1 Litre (8011)
A presterilised rigid polypropylene bottle ol appropriate capacity to collect water for
testing.
A. Bottle SPecifications:Polypropylene, *iO.
'o'if'' reagent bottle with built in seal ring to prevent leakage'
manfd. bY PolYlab'
Dimension in millimeters:
Dimension 8125 B01L
Base Diameter 40 74
Body Diameter 50 95
Heignt iaase to CaP) 100 210
Mouth Diametel 30 45
B. Bottle PreParation:
l.Bottlesarecleanedwithliquidsoapandwaterandfinal|yrinsedwithdistilledwaier.
2.Aqueoussodiumthiosulfatesolution(100gm/litre)dropsareadded,3dropsfor8125, and 20 droPs for B01L'
3. Bottles are then autoclaved at 15 lbs pressure at 1210c for about 30 minutes'
labelled and placed in low density polyethylene bags'
4'Labellingincludessterilizationbatchno.,date,aSpaceforwritingsamplecollectiondate, source etc'
C. Product Contents:
1. One presterilised polypropylene bottle of appropriate size'
2. One dark polythene bag of appropriate sae'
3. Test Requisitio. i.tt"iinrii and water sample collection Record (wcRl) Form'
4. Residents(RSCfi';ilil;rmer's (CGSCI j Guide for Water Sample Collection'
5. Tissue paper to wipe spillage if any'
D. Can be used to collect water tor various tests as shown below'
8125
Bacteriological AnalYsis (BCT)
Fluoride AnalYsrs of Water (FLD)
Ground Water Bacteriological Contamination Te$ (GBT)
BO1 L
Basic Potability Test (BPT)
Complete Potability Test (CPT)
Ground Water QualitY Test (GQT)
1 These boitres can arso be used to coilect water,f:i:lTlr11]:1t:*fl1l [?']3|ilt1ll;iltr;,:::Hffi ;ffi;;"#;Ji,-'.*t bergre usl
1o,tf :::::l':"':::::If:f;."yff [:i:T J',l:'+il: ffi d;; ;ffi 'fi 1+1 'Jrl I :: :1,:*: 11;ye
r tecn n i cat
il:ilffiil; ,n. .ontttnto laboratory about the bottle preparation process'
labware,A.K Scientilic tndustries 5531/9'Basti Harphool singh sadar
Delhi-110 0062. PolYlab Plastic
Thana Road,
]E **i**, "*+uoo'u-ty
The IHS 'oto'n*un
Hvde'abad AP ' 500 004 lndia 2
les - 125 ml (8125), and 1 Litre (8011)
ylene bottle of appropriate capacity to collect water for
reagent bottle with built in seal rrng to prevent leakage,
BO1 L
liquid soap and water and finally rinsed with distilled water.
fate solution (100 gm / litre) drops are added, 3 drops for
BO1 L.
tved at 15 lbs pressure at 1210C for about 30 minutes,
lw density polyethylene bags.
zation batch no.,date,a space for writing sample collection
lpylene bottle of appropriate size.
of appropriale size.'RL1)
and Water Sample Collection Record (WCR1) Form.
Consumer's (CGSC1) Guide for Water Sample Collection.
illage if any.
iraler for various tests as shown below.
BO1 L
) Basic Potability Test (BPT)
LD) Complete Potability Test (CPT)
amination Test (GBT) Ground Water Quality Test (GQT)
jto collecl water for similar testing by any other laboratory.
",rned laboratory before using these bottles for submission
Vater Quality Laboratory will be happy to answer technicallaboratory about the bottle preparation process.
Scientific industries 5531/9,Basti Harphool singh sadar
ry, The lHS, HACA Bhavan, Hyderabad. AP - 500 004, India. 2
Basic Potability Test (BPT)
This test checks for presence of bacteria, and indicators of contaminalion with putrefying
material. Fluoride level is also checked. This is a cost-effective means to reassure yourselfwhether the water consumed by you, your {amily and colleagues is potable or not. But thetest will not help much to investigate the source of contamination.
A. Sample Required:At least 500 ml of water collected in Water Collection Bofile - 1000 ml (B011), or
equivalent, delivered at laboratory preferably within 6 hrs, but nol later lhan24 hrs from timeof collection. Keep away from sunlight. Avoid exposure to excessive heat. Keep in refrigerator.
if delayed delivery is anticipated.
B, Test Duration:lf sample is found satislactory, then report will be available after 48 hours. ll sample
is found to have bacleria then further test will be made to look for indication of fecal
contamination. This test will take another 48 hours. So report will be available after 96 hours,
C, Report Details:
1, Most probable number ol organisms (MPN) present per 100 ml of water. This is an
indicator of bacterial contamination.
2. Physical indicators of contamination: PH,, Color. Turbidity, and Electrical Conductivity
3. Chemical lndicators of Contamination:(a) Ammonia, (b) Nitrites, and (c) Nitrates
4 Fluoride content. This indicates risk of fluorosis.
5. Total Hardness which includes Calcium and Magnesium.
6 Confirmatory test for E.Coli which indicates lecal contamination This test will be done
only if the MPN value is found unsatisfactory.
7 . lnterpretation is based on test results and sample collection record.
Proper Use of Water For Hygiene And Drinking
8125
40
50
100
30
/4
95
210
45
Mctst itrJectioLts agenls thor t'uusa clinrrhoeu arc lrunsmittetl bt, .t'oecal-or.ulrctute.'fltis itttludes transmission by corttominatt'tl drinking h'utet.ot.contdtltitldte(l .food,urtd.fron1 pet',\otl to per:;otl. C)leun v,utct. is essentiul .for.drinl;ing and tooking. ln uclclitiort. plentiful ol woter helps to an{'ouragahvgienic prtc'tices sut:h os hancl washing, t,leanirt.q o.f utensils, orttl t:lt,oningof'lotrirrcs. Thet^a prtrctict,s t'an itrtcrrLtpt the spretrd o.f infet.tious agentslhat tuu.st rliarrhoca. To.fltcilitutc gctotl ht,giena. it is rnore inqtorturtt thut
: tha v,oler suytply bt obutttlart than cleun, altlruugh both qualities ttre: desiruble.l-louseluLlds tltot hare ready acr.ess to o generot.ts supplt,of w,uter,: ancl to cletrn u,ttter.for clrinkittg ntrtl tookirtg, huye tliurrhoea le ss.ft'equenrly
thuttJonrilit,s v'ho"tt'd(((ss to tt'utcr is dill'it.ult or v,hos( drinking n,utcr i.s
, lteut'ily contuntinutctl.
IE warer QJalrty ,.r, nn *O*r. T-e lHS, rIACA Bhavar. rlyderabao. Ap - 500 00a. l"d:a. 3
)r
\,r
to Cap)
ter
iiiill;i;i::iiiliiiliiiiiill.'i:ilili.lll,.,r,,,'.r Complete Potability Test (CPT) ,,,,, ..'
This test checks for more water quality parameters compared to the basic potability
test and in addition, provides clues for possible sources ol contamination, ii any. Thus lhe
test not only helps you know if the water consumed by your lamily and friends is potable
but will also help investigate the possible source o{ contamination if any.
A. Sample Required:At leasl one litre of water is collected in Water Collection Bottle 1000 ml (B011) or
equivalent, delivered at laboratory preferably within 6 hrs, but not later lhan24 hrs {rom time
of collection. Keep away from sunlight. Avoid exposure 1o extensive heat. Keep in refrigerator,
if delayed delivery is anticipated.
B. Test Duration:lf sample is {ound satisfactory, then report will be available atter 48 hours. lf sample
is found to have bacteria then further test will be made to look for indication o{ fecal
contamination, isolate specific organisms. This test will take another 48 hours. So report will
be available after 96 hours.
C. Report Details:
1. Most probable number o{ organisms (MPN) present per 100 ml of water, This is an
indicator of bacterial contamination.
2 Tests to investigate possible source of bacterial contamination, These tests will be done
only i{ the MPN value is {ound unsatisfactory.
i. Confirmatory test lor E Coli which indicales {ecal contamination.
ii. lsolation of speci{ic bacteria such as Salmonella, Fecal Streptococci.
3. Physical indicators o{ contamination: PH', Color, Turbidity, and Electrical Conductivtty.
4. Chemical Indicators of Contamination:(a) Ammonia,(b) Nitrites,and (c) Nitrates
5. Mineral Content Estimation, These test will assess whether the level of naluraily
dissolved minerals are within permissible limits.
i. Fluoride content. This indicates risk oi fluorosis.
ii. Calcium, Magnesium, Sulfates, and Chlorides
6 lndicators ol lndustrial Pollution: Alkalinity Tesl
7 Quality of Treatmenl Assessment.
8. lnterpretation, based on test results and sample collection record,
1E Water Quality Testing Laboratory, The lHS, HACA Bhavan, Hyderabad, AP - 500 004, Indra 4
lte Potability Test (CPT)
vater quality paramelers compared to the basic potability
es for possible sources of contamination, if any. Thus the
he water consumed by your family and friends is potable
possible source of conlamination if any.
i collected in Water Collection Botlle 1000 ml (8011) or
preferably within 6 hrs, but not later Ihan 24 hrs f rom time
ight. Avoid exposure to extensive heat. Keep in refrigerator,
cry, then report will be available after 48 hours. lf sample
lurther test will be made 1o look for indication of fecal
anisms. This lest will take another 48 hours. So reoort will
'ganisms (MPN) present per 100 ml of water. This is an
nation.
source of bacterial conlamination. These tests will be done
nd unsatisfaclory.
Coli which indicates fecal contamination.
teria such as Salmonella, Fecal Streptococci.
tination, PH,, Color, Turbidity, and Electrical Conductivity.
mination:(a) Ammonia,(b) Nitrites,and (c) Nitrates
These test will assess whelher the level of naturally
permissible limits.
tdicates risk of fluorosis.
iulfates, and Chlorides.
on: Alkalinity Test.
nent.
results and sample collection record.
cry, The lHS, HACA Bhavan, Hyderabad, AP - 500 004, lndia. 4
Bacteriological Analysis (BCT)
Basic potability or Complete polability lests will establish bacteriological and chemical
characteristic of waler from a source. Usually the chemical characteristics of a source does
not change so much. Hence, there is no need to repeat the chemical characleristics at short
intervals. This test is same as the bacteriological component of the basic potability test, This
test is recommended to enable you to monilor bacteriological contamination of water al
frequent inlervals for a source for which basic potability test has been done at least once.
The test helps monitor contamination due to seasonal changes. And also to assess the
quality of lreatment.
A. Sample Required:100 ml of water is collecled in 125 ml (8125) water sample collection bottle or
equivalenl, delivered al laboratory preferably within 6 hrs, bul not laler than24 hrs {rom time
of collection. Keep away {rom sunlight. Avoid exposure to excessive heat, Keep in ref rigerator,
if delayed delivery is anticipated.
B. Test Duration:lf sample is found satislactory, then reporl will be available after 48 hours, lf sample
is found to have bacleria then further tesl will be made to look for indication of fecal
contamination. This test will lake another 48 hours, So reporl will be available after 96 hours"
C. Report Details:
1. Most probable number of organisms (MPN) present per 100 ml of water. This is an
indicator of bacterial conlamination.
2. Confirmatory test for E.Coli which indicates lecal contamination. This test will be done
only if the MPN value is found unsatisfaclory
3. lnterpretation is based on test results and sample collection record.
Potable WaterPrttultle n'utt't'is y)at(,r t't'lti(lt is./it .frtr corrsurtrlttion by huntunsttrtd olhcr ttninuls. It i,s ulso Lalled drittking r,oter, in a rc.f'ercnce
lo il.\ intcildcd use. \Vuter nruy be ttttturally potnblt, us is tltat tlst' v'itlt pristirtc s1'l'iugs, or it nru\, rrcetl to ltc trcutetl in ortlerto bt sale. ln cillrt,t'instdn(c, thc sulcty tf't'atcr l.s a.lsc.l-.scrl l,rllrte sls v'hi(lt look .l'or potentiollt lrurnrlirl contdnlinonts.
1E Water Quality Testing Laboratory, The lHS, HACA Bhavan, Hyderabad, AP - 500 004, India.
Bottled Water Integrity Test (BIT)
This is a basic test to check for integrity of packaged water. Generally yeast and molds
(fungus) would grow, if the bottling (packaging) process is delective, or the packed (bottled)
water has been stored longer than its designed life. Hence this test checks for presence of
Yeast & Moulds in bottled water.
A. Sample Required:
Sealed packaged water sample with name of the brand, batch no, date of expiry. Should
be delivered at the laboratory with sample collection record.
B. Test Duration:
It takes 168 hrs to confirm the presence or absence of yeast and moulds in water
sample because it takes nearly seven days 'for fungus to grow .
C. Heport Details:
1. Whelher Yeast and Molds are present or absent.
2. lnterpretation based on test result details in lhe manufacturer's label and sample
collection record.
Fluoride Analysis of Water (FLD)
Excess fluoride gives rise to a condition called Dental Fluorosis in children and Skeletal
Fluorosis in adults. Ground water from borewells etc may contain excess fluoride. This
Fluoride analysis of water test estimates the quantily of fluoride in waler and determines ii
it is within tolerable limit
A. Sample Required:1. Atleast 100 ml of water collected in a glass or polypropylene bottle.
2. Domeslic glass bottles can be used. Clean the bottle with soap water, rinse in plain
water and dry it before use. Fix an ordinary self adhesive label to note sample
collection date, time and source.
3. Or use IHS water collection bottle 125 ml (8125).
4. The sample should be accompanied by a Water Sample Collection Record (WCRI)
and a Test Requisition Letter (TRL1).
B. Test Duratioh: 24hrs.
C. Report Details:1. Fluoride content in water as mg per litre.
2. lnterpretation, based on test results and sample collection record.
IIS *u,., orr*y T.rtrg ,*ry The tHS. HACA Bravan. Hyderabad. AP - 500 004. lrdia. 6
Ground Water Quality Test (GQT)
This test checks for Physical and Chemical parameters of ground water. This is a
cost-effective means to reassure yourself whether the ground water consumed by you, your
family and colleagues is potable or not.
A. Sample Required:Alleast 500 ml of water collecled in a glass or polypropylene bottle. Domestic glass
bottles can be used. Clean the bottle with soap water, rinse in plain water and dry it before
use. Fix an ordinary self adhesive label to note sample collection date, time and source.
The sample should be accompanied by a Water Sample Collection Record (WCRI)and a
Test Requisition Letter (TRL1).
B. Test Duration: 24 hrs.
C. Report Details:
1. Physical indicators of contamination: PH,, Color, Turbidity, and Electrical Conductivity.
2. Chemical Indicators of Contamination: (a) Ammonia, (b) Nitriles, and (c) Nitrates
3. Mineral Conlent Estimation. These test will assess whether the level of naturally
dissolved minerals are within permissible limits.
i. Fluoride content, this indicates risk of fluorosis.
ii. Calcium and Chlorides, effect domestic use, tasle and palatability.
iii. Magnesium and Sulphates can be dangerous 1o mucus Iining causing gastro
intestinal irritation.
4. lndicators o{ lndustrial Pollution: Alkalinity Tesl
5. lnterpretation is based on tesl results and sample colleclion record.
1E Water Quality Testing Laboratory, The lHS, HACA Bhavan. Hyderabad, AP - 500 004, lnda. 7
.i.i..t.,t,Water Qaality
Wute r tluolit,- is thc phv-sit'ul, r:hcntical ancl biologit'alt:lturaclt,ristics rll v'otcr. ll is ntost.frequently Lrsed lt-t,
rcf ercnce to a set o.f stundards aguirtst which con4tliatk'e cotlbe assesse cl. Tlte ntosl Lonmlon stondut'tls t.tsed to asse ss h,ate rquttlilv relote to tlrinkin,q n'oler. salett tt ltuntatt t:ttrttar:t and
./or tke lrealrh of'(cos\stcms.
ical
lf borewell is near to sewerage, septic tank' place ol open defecation then there is a
possibilityolfecalcontamination.Usuallythechemicalcharacteristicsofasourcedoesnot
changesomuch.Hence,thereisnoneedtorepeatthechemicalcharacteristicsatshort
intervals.ThetesthelpStomonitorbacterialcontaminationduetoseasonalchanges,and
also to assess the quality of treatment'
A, SamPle Required:
Atleastl00mlofWateriscollectedinl25ml(8125)watersamplecollectionbottle
orequivalent,deliveredatlaboratorypre{erablywithin6hrs,butnotlaterllan24hrsfrom
timeofcollection,Keepawayfromsunlight.Avoidexposuretoexcessiveheat.Keepin
refrigerator, if delayed delivery is anticipated
B. Test Duration:
lfsampleisfoundsatisfactory,thenreportwillbeavailableafter43hours.llsamp|e
isfoundtohavebacteriathenfurthertestwi|lbemadetolookforindicationo{fecal
contamination, isolate specific organisms. This test will take another 48 hours. So report will
be available after 96 hours.
C. RePort Details:
1. Most probable number o{ organisms (MPN) present per'100 ml of water This is an
indicator o{ bacterial contamination'
Teststoinvestigatepossiblesourceo{bacterialcontamination.Thesetestswillbedone
only if the MPN value is tound unsatisfactory
i. Confirmatory test for E Coli which indicates fecal contamination
ii. lsolation of specific bacteria such as Salmonella' Fecal Streptococci'
lnterpretatlon is based on test results and sample collection record'
hS Water Ouality Testrng Labo'atory Tne IHS HACA Bhavan Hvde'abad AP - 500 004 lndra B
2.
ical
septic tank, place of open defecation then there is a
the chemical characteristics of a source does not
no need to repeat the chemical characteristics at short
bacterial contamination due to seasonal changes, and
in 125 ml (B125) water sample collection bottle
preferably within 6 hrs, but not later lhan 24 hrs from
sunlight. Avoid exposure lo excessive heat. Keep in
then report will be available after 48 hours. lf sample
test will be made lo look for indication of fecal
This test will take another 48 hours. So report will
(MPN) present per 100 ml of water. This is an
of bacterial contamination. These tests will be done
unsatisfactory.
which indicates {ecal contamination.
such as Salmonella, Fecal Streptococci.
results and sample collection record.
The lHS, HACA Bhavan, Hyderabad. AP - 500 004, India. B
This test checks for more quality parameters compared to the Ground water quality
test and in addition, provides clues for possible sources oi contamination if any. Thus this
test not only helps you know whether the ground water consumed by you, your family and
colleagues is potable but will also help investigate possible source of contamination if any.
A. Sample Required :-
At least one litre of water collected in water collection Bottle 1000m1 (8011) or
equivalent,delivered at laboratory preferably within 6 hrs,but not Ialer than 24 hrs {rom time
of collection. Keep away trom sunlight. Avoid exposure to excessive heat. Keep in refrigerator,
if delayed delivery is anticipated.
B. Test Duration :
lf sample found satis{actory,then report will be available after 48 hrs.lf sample is {ound
to have bacteria then lurther lest will be made 1o look for rndication of fecal contamination,
isolate specific organism. This test will take another 48 hours. So report will be available
alter 96 hours.
C. Report Details:
1. Most probable number of organisms (MPN) present per 100 ml of water. This is an
indicalor of bacterial contamination,
2. Tests to investigale possible source of bacterial contamination. These tests will be done
only if the MPN value is found unsatisfactory.
i. Confirmatory test for E.Coli which indicates fecal contamination,
ii. Isolation of specific bacteria such as Salmonella, Fecal Streptococci,
3. Physical indicators of contamination: PH,, Color, Turbidily, and Electrical Conductivity.
4. Chemical lndicators of Contaminalion:(a)Ammonia (b)Nitrites, and(c) Nitrates
5. Mineral Content Estimation. These test will assess whether the level of naturally
dissolved minerals are within permissible Iimits.
i. Fluoride content, this indicates risk of fluorosis.
ii. Calcium,Magnesium,Sulfate,lron and Chlorides.Magnesium and Sulphates can be
dangerous to mucus lining causing gastro intestinal irritatton.
6. lndicators of Industrial Pollution: Total Dissolved Solids (TDS) and Alkalinity ensure in
decreasing lhe potability, continuous exposure can lead to Gastrointestinal irritation,
7. Quality of trealmenl assessment.
8. Interpretation,based on tesl results and sample collection record.
]EWaterQua|ityTestingLabo.atoryThelHS'HACAB'ravan'n,o.-u,
chlorination by addrtion of a disinfectant rbreaching powder or riquid chrorrne; is the
::Til.ff;lffi'J:,,:;:I:::T::111:Loll!n,:n^,,recliveness or tr,is met oo ilo.penoenton the avairabirity of chrorine in rhe disinfectrrr i,**,r, ffi# ffi't;l:ffiriT::',t#basis of available chlorine.
_ Expected chlorine availability
Bleaching powderLiquid Chlorine
Water disinfectant
grade
Grade-1
Grade-234%Qaa/
4%-6%
125"/" - 15%
This test estimates the chrorine availabirity in a given sample of bleachrng powder orliquid chlorine. The test js usefur u, ,n ,...p[nl, i.o ,, the time of purchasing disinfectantsto determine whether the product r...,uui tio, a supprier satisfies the promised chlorine
il:litiy,J:i,LTJ;;;L:,,retur to caturate tni quantity or avairalre lisinrectant to oe
A. Sample Required:l Bleaching powder:Atreast 100 gms coilected using a dry plastic scoop and placed_ in a seated potythene (LDpE) picket2 Liquid chrorine;Atreast'100 ml collected in a dry au tight prastic bottle3 Both: Deriver the product rn its originar pu.r,rgrl rn this case a sampre can be takenby the laboratory for testrng and re-st ot ir.,. pioor.t can be corected by the consumer,B. Test Duration: 24 hours.
Poor quarity or stored disinfectant may not ,r,irty ,t,, above chlorine avarlabirity.
il:filiJ iiil::::,,",.,i1::llectanr req;ir;;,r.,, p,.p., trearmenr of water is carcurared onthe basis of chro.ne avairabirirv rr,us iilo, ;;r; ffiffi:ftH.ilffi:Jfl::iiffi;
ffi*,i iJ:1[.:?l'#:#;irabiritv to i,.i r.*"*,, carcu rate lrre q;ffi ; ;; breachins
C. Report Details: The perceniage of available chtorine by votume.
Alum Quatiry Test (AeT)
Alum grade and expected AlLtmrna in oo
Grade-l 17%Grade-ll fi%
A. Sample Required
If surface water such as tanks, river, or lakewater is lhe source for water supply system,alum is used to remove turbidity. euality oi alumto be tested depends on jts ajumina content.
Minimum 100 gms of Arum in a seared poryethyrene (LDpE) cover. derivered atthe raboratory,with sample corection record or Derver the prodrri , n, original package, in tnrs case a samprecan be taken by the laboratory for testing and rest or tne flouct can be coljected by the consumer.B. Test duration: 24 hours
C. Report Detaits: Alumjna content in oo oy mass.
ItS .,-* --" , t:,,r,g L?o0rarory. Tl.e lHS. HACA Bhavan, Hyoeraoad. Ap - 500 00a, lnora. 10
n Quality Test (AeT);, river, or lake
upply system,
)uality of alum
na conlenl.
sealed polyethylene (LDpE) cover. delivered at the laboratory,ver the product in its original package, jn this case a sampleing and rest of the product can be coljected by the consumer.
ltent in % by mass.
Liquid Chlorine Acceptability Test (LAT)
Liquid Chlorine is commonly used in disinfection of drinking water. Effectiveness of
chlorination by addition of disinfectant (Liquid Chlorine) is dependant on the availability
of chlorine in disinfectant.Liquid Chlorine grade is assigned on the basis of available
chlorine.
Water disinfectant grade Expected Chlorine availabilily
in liquid chlorine
ine Availabitity Test (CAT)
rf a disinfectant (bleaching powder or liquid chlorine) is themeant for drinking. Effectiveness of this method is dependentthe disinfectant. Bleaching powder grade is assrgned on the
Expected chlorine availabilityBleaching Powderliquid Chlorine
34%
32%
4% 6%
12.5%. 15%Grade-1
Grade-2
4%-6%12.5% - 15%
rinfectant, may not satisfy the above chlorine availability.ctant required for proper treatment of water is calculated onThus, if you have old stock of bleaching powder, it would
lability to help accurately calculate the quantity of bleaching
crine availability in a given sample of bleaching powder orts an acceptance test, at the time of purchasing disjnfectantsct recejved from a supplier satjsfies the promised chlorineful to calculate the quantity of available disinfectant to be
00 gms collected using a dry plastic scoop and placedE) packet.
ml collected in a dry air tight plastic bottle.its original package. ln thjs case a sample can be takenand rest of the product can be collected by the consumer.
).
entage of available chlorine by volume.
The above chlorine availability may nol be satisfied in case o{ poor quality or stored
disinfectant. Based upon the chlorine availability, the quantity of disinfectant required for
proper treatment of water is calculated.
This test is designed to assist municipal, rural/urban water supply authorities in
acceptance testing of liquid chlorine al the time of purchasing. Liquid Chlorine bottles will
be randomly sampled following the specifications in lS 11673:1992
A. Sample Required:At least l00 ml of liquid chlorine collected in a dry air tighl plastic bottle. Deliver the
product in its original package.ln this case a sample can be taken by lab for testing and
rest of product can be collected by consumer.
B. Test Duration: 24 hrs
C, Report Details: The percentage of available chlorine by volume.
Aum graOe and expected Ajumina in 4Grade-l 17 %Grade-ll $%
r' E01c ot' HLrnun Diseuscs ilt ( Wdt?r Borile.
r' Childrert urtd tlu' Agt:tl huve o y,cakcr innturtitt' ,\r,.rrcrr. Henct thctdra nt()re sustt,ptiltlc to Wdter Bornc Discttst,s.
r' 'I'lre Wutt:r Ptrriliet-.s itr orrr honreslofJict,s ttrt,fttccd yrith cortstutr/ thrcotof clrotking of-lilters yyltich rctttlt,r tltcnr irtcff'ccriyc: this is a loontingtlttngcr to our heulth.
ry, The lHS, HACA Bhavan, Hyderabad, Ap - 500 004, India, ,10 1E Water Quality Testing Laboratory, The lHS, HACA Bhavan, Hyderabad, AP - 500 004, Ind a. 11
Why you should test your water?
Collection ServiceThe laboratory wiil depute a trained person to coilect waler sample from the crient
designated premises any where in Hyderabad District. The visit will be within seven days,according to mutually agreed day and time. The sample collector will come with lab preparedsample collection bottle, required stationary. (S)he will collect the sample, fill the water samplecollection bottle record, assist the client in filling up of the Test Requisition Le1er, and carrythe sample to the laboratory.
Collection Service
Same as above, but the response time will be within one business day.
Sample Collection
Same as above, but the response time will be immediate. Sample collector will reachas s00n as possible, or within 2 hours.
Survey of Source
The Iaboratory will depute appropriately trained personnel to visit a water source anddo sanitary survey of following types of sources using appropriate sanitary rnformatjon formdeveloped by the Institute based on wHo recommended formats, Forms wS-r to 6mentioned below are given in the IHS publication titles A manual on Control of Gastroenteritiswith special reference to Andhra pradesh, lndia , 200.1 by prasanta Mahapatra and SamathaReddy. These or their rarest updates. if any wilr be used as guide to the survey.
Water Source Type Sanitary
lnspection Form
Dug Well
Bore Well / Tube Well
Piped Water Supply System (pWS)
Water Tanker Truck Distribution System
lntakes from Reservoirs,Streams and Rivers
Water Treatment Plant
Service charge estimates will depend on the location of the source, number of sourcesin a cluster. the level o{ details required under general comments. and the nature of followup action expected by the laboratory.
water Quality Testing Laboratory, The lHS, HACA Bhavan. Hyderabad, Ap - s00 004, India. i2
WS-1
WS.2
WS-3
WS4
WS-5
WS.6
e Collection Service (SCS)
l a trained person to collect water sample from the clientr in Hyderabad Distrjct. The vjsit will be within seven days,y and time. The sample collector will come with lab preparedlstationary. (S)he will collect the sample, fill the water samplee client in filling up of the Test Requisition Letter, and carry
ple Collection Service (FCS)
sponse time will be within one business day.
ample Collection Service (ECS)
;ponse time will be immediate. Sample collector will reachhours,
'Survey of Source (SNS)
ppropriately trained personnel to visit a water source and)es of sources using appropriate sanitary information formd on WHO recommended formats. Forms WS_l to 6HS publication tjtles ,,A
manual on Control of Gastroenteritisradesh, lndia", 2001 by prasanta Mahapatra and Samathates, if any, wiil be used as guide to the survey.
Sanitary
lnspection Form
Clients Guide lor Preparation of Water Sample Collection Bottles
1 ldentify a suitable glass or polypropylene bottle
Bottle volume should at least be 20% more than the volume of samole.
Prefer a bottle with wider mouth"
The bottle's cap should be closed tightly.
iv. Used bottles are acceptable, but would require soaking and thorough cleaning.
Clean the bottle and its cap with soap water.Liquid soap is preferable lt using bar soap
make sure that no soap piece is lelt behind in the bottle
Rinse both bottle and cap in plain water adequately to remove traces oi soap.lf feasible,
use distilled water for the final rinse,
Dry the bottle and its cap. Avoid exposure to dust while drying.
Place the cap securely, after drying.
Fix an ordinary self adhesive label to note sample collection date. time and source. A
floppy disk label or a school note book label will do.
Use the bollle for collection of sample within four days of preparation.
Note that such a bottle can be used to collect water sample for tesling of physical and
chemical parameters. To check for presence of micro organisms and send a sample
for bacteriological analysis use an appropriate lab prepared (presterilised) bottle.
1.
ii.
iii.
2.
a
4.
r
6.
7.
8.
lr
)ystem (PWS)
tistribution System
rs,Streams and Rivers
t
WSl
WS2
WS3
WS-4
WS5
WS-6
Legal Provisions abottt water quality :
The Atdhra Pracleslt Public Hcalrlt (APPHA) Acr. l9-19,
J-i,res resportsibility- on the local oulhot'ity .fbt' ,t'oter sttltpl ,-. Tltusthe municillul arrthorities and Grunt Purtt'ltayrtts arc legallr-rctluired to provitle .fbr su/jic'ierrt s'u1t1tlt of tlrirrkittg v,ater.fttt'c:r.tnsrrntptiort by inhttltitants ctJ' tlrt: orao witltirt tlteir.jurisdic'tion.Under sectictn 20 the District Collectot' ltcts pox,ersto coLtse encluires db()ur :;dnitot'y contlitiotr of n'ater .ru7t1tlt,.s.),i\-r./x
urtd odecluttt'y of .su1tph,. Under' .yectiott-21 tlte Directr.tt' - PublitHealtlt has pov.,ers 1o clirect tt lctt:ctl ctulhot'itt,tt) intl)t't)t'.,\1'Ltt('t'sultply. Under section 21 tlte Heolth ()fficer L'utt {it','intrt'ttLtit)n,\to dny pet-,son hut'irtg t'ortltttl of'drinliin,q \'dter sourc(, ttt lnkeapltrr,tpriutc actiott to ntuitttain its hv.qicnc.
iepend on the location of the source, number of sources:ired under general comments, and the nature of follow
v.
t The lHS, HACA Bhavan, Hyderabad, Ap _ 500 004, lndia. 12 IE Water Qua,,, *n *Oo*,r,* lHS. HACA Bhava^. Hyoe.aoao. AP 500 004. Inora t3
:7A*/ ?/,6r/",,y
n.rf;:r;;;.important practice that minimisr-:.s rhe risk of gastroenteritis
t
Co,il hand washi
cleaning of ay parts ir*'r'nl*t use o'f soop' arl'etpate water' and' carefu
crearc a prace within the hctme for hand-washing. This shourd haue awater tap, bucket ctr dntm containing water, arLd. soap
An adurt or order sibring shcturd wash the hanrls of 10u,g chirdren.
%rr/'7%r/ %rr* q"hl- Wash hands
before eatmg.
rr- \Yash hands
before serving of foocl(r \Vash hands
befctre cooking,
(.r Wash hands
after defecating and ttbltttirnt.
Wash hands
after cleaning a child. who has clefecated, changrngnappies, ctr after dlgoslng o.f a chikl.,s stooL.
Wash hands
after using a dssue ctr hanclkerchief .
Wash hands
aJ'ter playing with 1tets.
tt= *u". o*,"*** *,.*ar, rn. ,ni noao ,nrran, Hyderabad, Ap - 500 oon ,no,r. t4
Water Quality Testing Service Charges
ServiceCharges/AD (Rs.)
B01L Water collection Bottle-1000 ml
ECH Electrical Conductivity or TDS & pH
BOT Basic Tests
au rii
Basic Potability Tests
:ii:g&ffi400
600BPT
=ffiFCPT
ffiTE-:,iGPT Ground water Potability Tests
SPT Surface water Profile Tests
SPB Surface water Proflle Tests plus Boron 1250-=B-90'=-BasClihotRiia=frrilffi uCtity:=ffiii.,.,.---€i:,.=.====-42SQ.,i
BRP Baseline Rivenrrrater Proflle Tests 1 500
,'.ffi ',
..Poolsidgte,st=€'*g1r.':'''.==..,, ., t'=..5CIt0'
Poolside Test GHMC Limits 700
=ft l., - .,Fniilfi ite r.qq.d i0- Teslsrr:.,,
=r -.-, =..= 5:j.69 ["'.
CSC City Sample collection Service 200
G5,6,'...6lraterHyd 5aoSrmpiemitbmm .- 400 .FCS Fast Sample Collection Service 1.5X
ECS Emergency Sample Collection Service gX
SNS Sanitary Survey of Source Casebasedeslimate
Note: Prices may be revised from time to time. Please ask forlatest price list at the time of ordering for test.
at
Jf, Water Quality Testing Laboratory The lHS, HACA Bhavan, Hyderabad, Ap - 500 004, lndia.
2.
J-
4.
Resident's Guide for Water Sample Collection.'L Select and secure the sample collection bottle and a dark coloured carry bag:
i. Depending on the test required, you may use either "client prepared" bottle or"lab prepared" bottle. Client prepared bottle means that the bottle can beprepared by you at home or in your establishment. Note that the bottle has tobe prepared sufficiently before you use it to collect a sample, so that there is
time for the bottle to dry. Lab prepared bottle means a presterilised bottleprovided by the laboratory.
ii. The Water Quality Testing Service catalogue shows where "client prepared"bottle is adequate for collection of sample. The laboratory may, however, prepareand supply a bottle for an additional service charge. Note that bottles areprepared with specific tests in mind. Hence check with laboratory before youuse a lab prepared bottle.
iii. Note that, for certain tests separate bottle would not be required. For example,to test packaged water, simply deliver the packaged water as it is to thelaboratory.
iv. Here sample collection bottle means thelab prepared bottle or client preparedbottle as appropriate.
Do not open bottle untill, ready to use.Do not rinse the bottle.Plan to collect water into the bottle in such a
way that the sample is representative of thewater to be tested.0pen the bottle gently and fill water withoutany splashes.
6. Tightly close the bottle. Wipe any spillage onthe outside of the bottle,
7. Write sample collection date, and brief identification of the source on the bottlelabel, using a permanent marker pen.
8. ln case of collections from a storage tank, collect the sample from below thesurface by gently dipping mouth of the bottle under the water gradually filling thebottle, so that you do not stir up the sediments, if any.
9. Place the bottle inside the darkcoloured polythene bag and putadequate ice cubes inside the bag. Thequantity of ice cubes should beenough to cover bottom 2/sd of thebottle. Excess ice above bottle's neckmay cause mixing up of the samplewith water from the ice. Make sure thatice cubes remain below the neck.
10.Fill in the Water Sample CollectionRecord [/VCR1l and the TestRequisition Letter ORLIJ. These formsare provided with the IHS lab prepared water collection bottles. They can also bedownloaded from the IHS web site at http://www.ihs.org.in/wqVindex.htm.
ll.Deliver the sample at the laboratory as soon as possible, preferably within sixhours, and definitely not later than 24 hours from the time of collection.
12.Keep away from sunlight. Avoid exposure to excessive heat. Keep in refrigerator ifdelayed delivery is anticipated. Always keep the bottle in upright position.
\\?
,&-=9dTil\\ ll ,.'/Vl tl
e l__"nl+ A-'t.I L-1 L'.- -mtsl-l ./!]rJ/'\'
ll/{<JtL--......-J ,/ /i
Citizen Actian forGood Water Suppb and Safe Sewerdge*,
Watchout for DefectiveWater Supply and Sewerage Systemsl!
Effectiveness of household water supplyand drainage is ultimately linked to thewater supply and sewerage systemmaintained by your local body (Panchayat,
Panchayat Engineering department,Municipality, Water and Sewerage Board,
as the case may be).
As responsible citizens, you can watch
for defects or damage to water supply and
sewerage system in your atea.
You have a direct stake in proper
maintenance of these facilities.
So report any defect to your local
authority.
Water Quality Testing LaboratoryThe lnstitute of Health SystemsHACA Bhavan, Hyderabad,AP- 500004, lndia.
Tel:91 (40) 2321101314;tax:91 (40) 23241567
Email: [email protected] (or) [email protected]
http:/iwwwihs.org.in/wqt
