CARY 100 Brochure

UV-VIS-NIR Varian Cary 100/300/400/500Spectrophotometers

Cary100_500Bro_PDF 22/11/00 4:11 PM Page 1

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High performance, flexibility and ease-of-use are justsome of the words that are associated with the Caryrange of UV-Vis-NIR spectrophotometers. If you want to invest your money in an instrument that meets all ofyour needs today, as well as future requirements that youmay not have even thought of yet, feel confident that a Cary is that instrument. The Cary range is extensive,with instruments to suit most budgets and applications.

Cary 50* This innovative instrument represents thebudget end of the Cary line.

Cary 100 A low to mid-priced instrument, the Cary 100is suitable for routine and research laboratory work.

Cary 300 represents a cost effective, research-gradeinstrument for laboratories doing analyses of biologicalor highly absorbing samples.

Cary 400 This research-grade instrument represents the top-of-the-line UV-Vis spectrophotometer. (Optional deep UV version also available.)

Cary 500 extends the capabilities of the Cary 400 up to 3300 nm. If you want an instrument that will measure any sample then the Cary 500 is the best choice.

Cary Eclipse Fluorescence* spectrophotometer compliments the Cary UV-Vis range by offering the first truly new mid-range fluorescence instrument in more than 10 years.* Refer to separate brochure for details

Cary—the instrument for today & tommorrow


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Applied Physics Corporation (known later as Cary instruments) was founded in January, 1946, by Howard Cary, William W. Miller, George W. Downs, and Russell E. Vaniman. Howard Cary made important contributions to analyticalinstrumentation in the mid 1930s and early 1940s. He founded Cary instrumentsjust after leaving his position as vice-president in charge of development withBeckman Instruments, where he had beenfor 11 years.

In April 1947 Applied Physics Corporationdelivered the first commercial recording UV-Vis spectrophotometer, a Cary 11, to Mellon Institute in Pittsburgh, USA.

At that time Applied Physics Corp had only 12 employees.

In 1954 the Cary family grew to include the Cary 14.

By that time the Cary name had become synonymous with high performanceand reliability.

In 1966 Cary merged into Varian and in1972 the Cary operation moved fromMonrovia to Varian’s main facilities in PaloAlto, California. In 1982 the Cary operationwas moved to the Varian Techtron facility in Melbourne, Australia. During the mid tolate 1980s the Melbourne based factory wasvery busy researching and developing awhole new generation of Cary instruments.

Using the best optical and performancecharacteristics of the past Cary instrumentsand the latest technology in electronics and software the Cary 1, 3, 4 and 5 werecreated. On the 50th anniversary of the first Cary a whole new series of Caryinstruments, the Cary 50, Cary 100, Cary300, Cary 400 and Cary 500 were born. In 1999, the Cary range was extended toinclude the Cary Eclipse fluorescencespectrophotometer, offering the excellentspecifications and modern software that has become the Cary standard.

1947 1954 1961 1964 1970 1972

Cary 11 UV-Vis

Cary 14 UV-Vis-NIR

Cary 15 UV-Vis

Cary 16 UV-Vis

Cary 17 UV-Vis-NIR

Cary 118 UV-Vis

For investigators who on occasion must push a spectrophotometer to the very limits of its

performance capability to obtain the information theyneed, and yet have to have an instrument which is adaptable to many different applications“ ”Howard Cary

The Cary Philosophy 1947

Fifty years ofexperience, startingwith the Cary 11, serialnumber 2, circa 1947.

Cary—More than 50 years of excellence


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• If you purchase the Tele-diagnosticsoption then Varian can test your instrumentremotely to try and find the fault. This savesyou money and gives you access to Varianexperts—no matter where they are.

• Varian’s website contains manyapplication notes. You can use these to find out how to measure a particular sample or how to optimize the use of your Cary. A visit to the website will also give youinformation on the latest software andhardware releases.

• If you need parts or supplies for your Cary then our website has an extensive list.If you aren’t connected to the Internet thenyou can receive the parts and suppliescatalog on CD ROM.

1975

Cary 17DUV-Vis-NIR

1977

Cary 219UV-Vis

1987

Cary 2400UV-Vis-NIR

1989

Cary 1 & 3 UV-Vis

1990

Cary 4 UV-Vis Cary 5 UV-Vis-NIR

1997

Cary 50 SeriesCary 100-500 Series

1999

Cary Eclipse Series

You’re not just buying a Cary, you’re buying a relationship But what if you need help with your Cary instrument? Thanks to the wonders of our electronic age there are many options and sources of information foryou to access, including:

• Free access to other Cary users around the world via an Internet mailing list. If you have access to email then you can ask any question you like, for example, ‘Has anyone measured this type of samplebefore?’ Your email will be automaticallysent to all other Cary users who subscribe to the list. Experts at Varian also belong to this list, so you are assured of getting the answer you need. Varian also uses the mailing list to notify users of newproducts, new application notes and other useful information.

• You can call your Varian office and get over-the-phone support or request anon-site service call.

Varian on the Net http://www.varianinc.com Varian maintains a comprehensive website. To go directly to the UV-Vis-NIR section, visit http://www.varianinc.com/osi/uv


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Although the Cary 100and 300 are doublebeam design you canelect to operate themin single, double ordual-single beam modes

Quartz overcoatingprotects the opticsfrom the environmentand allows cleaningwithout damage totheir reflective surface

Variable slits provideoptimum control overspectral resolution

The AccessoryController offerscentralized accessorycontrol. This makes iteasier to service theinstrument as there is only one set ofaccessory electronics.You can alsocommunicate withnon-Varian accessories via the Accessory Controller

Double choppers ensure that the sampleand reference beam strike the detector at the same point, removing any errors due to nonuniformity of the detector

A phase lockedwavelength driveprevents peak shifts and peak suppression at high scan speeds

The patented opticalsystem eliminatesresidual chopperwobble, preventing any image movementon the detector

The large samplecompartment gives you more flexibility in sample size

The sealed opticsprevent exposure tocorrosive environments

The Cary 300 has a premonochromator,extending its rangepast 5.0 Abs

Cary 100 & 300 Cary 400 & 500


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The PbS detector isthermoelectricallycooled to 0 °C to reducephotometric noise

The optics are silicaovercoated, so thatthey can be cleanedwithout damage tothe reflective surface

The Cary 400/500wavelength drive canchange wavelengths at16 000 nm/min in theUV-Vis and at 64 000nm/min in the NIR

The lamp turret hasprovision for a thirdlamp of your choice.The turret can becompletely removedif you wish to installanother light source

A big samplecompartment, with aremovable floor plate,gives you maximumflexibility whenmounting samples

The samplecompartment windowsare tilted to preventback-reflection andthus incorrect results

The slits can be fixed in the NIR as wellas the UV-Vis

The out-of-planedesign of the doubleLittrow monochromatorreduces photometricnoise and stray light,and produces excellent resolution

Schwarzchild couplingoptics ensure a high level of light throughput.This produces moreaccurate measurementsat low transmissionlevels

Electronics are also a very important design consideration.The extended dynamicrange of the Caryinstruments is a resultof this. The Cary 400 and 500 instrumentsfor example, arespecified to measure upto 7 Abs with referencebeam attenuation

A ‘floating’ solidaluminium castingisolates the optics fromexternal disturbances

The monochromator andsample compartmentshave separate nitrogen purging plumbing

Cary 400 & 500


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The Cary Win UV software has a modulardesign which means that you are onlybuying the functionality that you need. It also means that if your use of thespectrophotometer changes in the future it is simple to upgrade to meet your newrequirements. Of course, the Cary Win UVsoftware takes advantage of Windows®

32-bit functionality such as multitasking andfile association, for example you can dragand drop a data file onto a Cary applicationand it will be immediately loaded.

Several Cary packages are available for themost common UV-Vis-NIR applications.These packages bundle together appropriatesoftware modules with an instrument to suit each application.

The three general use packages are Scan,Conc and Bio. Packages are also available for Tablet Dissolution, Color Analysis, Ultra-Violet Protection and the list iscontinuously growing. Of course, you canthen add any of the Cary accessoriesthat you need.

Hint textHaving trouble figuringout what a particularbutton or control does?Just hold the mousepointer over the objectuntil the hint text popsup with an explanation.

Auto load iconsJust click on theappropriate icon and the application and the correct method willload, ready for you to measure samples.

Cary Win UV software—just what you asked for


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Single window operationAll controls can beaccessed from thiswindow, making operationquick and easy.

Graphics tool barThe Cary softwarefeatures a toolbar to giveyou quick access to themost common graphicsoperations such aszooming, adding text andchanging the axes ranges.

The Cary WinUV comes with the inbuiltApplications DevelopmentLanguage (ADL), a computer languagewhich can be used toautomate yourlaboratory'smeasurements. You candownload a range ofcustomized ADL programsfrom Varian's website ordevelop your own.

The Auto Convert function allows you toautomatically convert your data files for use in another program. You can also elect to store your data files informats that can bedirectly imported into a spreadsheet.

Batch filesYou can store all methodand report parameters,calculations and graphicaldisplays, and the actualdata in one file. This savestime as you simply recallone file instead of several.

The Cary WinUVsoftware includes step-by-step videos ofhardware installationand use.


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Scanning—any way you wantThe Cary Scan package is available foreach of the Cary 100, 300, 400 and 500instruments. It consists of the followingsoftware modules:

• Scanning software with Maths module• Simple Reads module• Advanced Reads module• Instrument Validation module• GLP module for file security• Applications Development Language (ADL)

Cary 100/300/400/500 Scan

Making simple things simplerThe Simple Reads application is an example of how the modular conceptof the Cary software makes day-to-daymeasurements easy. Modular softwaregives you the advantage of only having todeal with controls that are appropriatefor what you want to do.

For example, to measure a sample at asingle wavelength, simply do the following:

Step 1 Double click on the Simple Reads icon on the desktop.

Step 2 Click on the Setup button, set the wavelength.

Step 3 Press the Zero button.

Step 4 Press the Read button.


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What makes Cary wavelength scanning so good?Most spectrophotometers can scan, it’s abasic function. However, in addition to thebasic requirements of wavelength accuracyand a wide dynamic range, the Caryinstruments offer a host of special features:

Independent NIR controlThe Cary 500 enables you to independentlycontrol the parameters in the UV-Vis andNIR regions so you don’t have tocompromise when scanning across thewhole range. For example, if you want more detail in the NIR without sacrificingmeasurement time, just set a narrow SBWand small data interval in the NIR and a wider SBW and larger data interval (andthus a faster scan speed) in the UV-Vis.

Multiple ordinate modes Instead of collecting your data inAbsorbance or %Transmission and thenconverting it to the mode that you want,you can save time by collecting the datadirectly in the ordinate mode you need.Choose from: Abs, %T, %R, Absorptivity, Log Abs, Kulbelka Munk F(R), Log KubelkaMunk Log F(R), Log (1/R)

Flexible abscissa modesSelect from several abscissa modesincluding: wavelength, interval scanning(collecting data every 5 nm) and linearwavenumber scanning

The Cary 500instrument allows youto independently controlthe parameters in theUV-Vis and NIR regions,including the spectralbandwidth, data intervaland averaging time. This gives you completecontrol over the quality of the data and thespeed of its acquisition.Signal-to-noise mode

is a unique methodof collecting dataavailable only on theCary instruments. It allows you to control the level ofprecision you wantacross the whole scan.

With the Cary Scan system you can scandirectly in wavenumber (cm -1) (lower scan). The instrument is drivenso that the wavenumberinterval is constant, rather than collectingthe data in wavelengthand then convertingit to wavenumber, withthe resulting unevenspread of data points(upper scan).

This saves time becausethe system will scanquickly in areas of highenergy throughput andslow down in areaswhere the energy isless. Shown here is thesame sample measuredwith (bottom) andwithout (top) signal-to-noise mode.


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Why is a Cary so good for Life Science measurements? The Cary Bio package is available for each of the Cary 100, 300 and 400instruments. It consists of the followingsoftware modules:

• Simple Reads module • Advanced Reads module• Scanning module• Kinetics module• Enzyme Kinetics module• Scanning Kinetics module• RNA/DNA Estimation module• Thermal denaturation and

renaturation module• Instrument Validate module• GLP module program for file security• Applications Development Language (ADL)

These packages are ideal for the multi-user,multi-discipline Life Science laboratories.

Stirring is smooth and easy to control The stirring mechanism of the Cary peltierthermostatted cell holders relies on a rotating magnetic field, generated by analternating electric current. This ensuresreliable operation with no fluctuations instirring speed and allows complete control over the speed of the stirring. Up to 12 cells can be stirred at a constantrate for extended periods of time.

Excellent temperature control The temperature control of the peliter thermostatted cell holders (either the multicell or single cell versions) is extremely stable over time, with a typical variation being ±0.05 °C. The cell to cell variation is also minimal with a maximum difference of 0.2 °C at 37 °C. The temperature inside the cuvette can be monitored with the Temperature Probe accessory.

The Temperature Probeaccessory allows themeasurement of thetemperature inside the cuvette.

The Cary’s focussed beam and high energythroughput means thatvolumes as small as 2.5 µL can be measuredaccurately. A range ofultra-microcells and semi-microcells areavailable from Varian.

Cary Bio—leading instruments for life science


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The Diffuse ReflectanceAccessory is idealfor turbid solutions. The scattered light isgathered by theintegrating sphere which minimizes thelevel of ‘noise’ inyour data.

The RNA/DNAEstimation applicationhas built in methodsthat enable you todetermine the purityand the type of nucleic acid present in your sample. Warbug and Christiancalculations are alsoavailable for nucleicacid and proteinquantification. (Top.)

Enter sampleinformation such as the pH, ionic strength,etc., in the User DataForm. This informationis then stored with thedata file. (Middle.)

The optical design of the Cary instrumentsensures excellentphotometric stability.This means that anyabsorbance change youobserve during akinetics run is due onlyto the sample, not todrift in the instrument.

Data security guaranteedSome spectrophotometers only store dataat the end of a run, so if the power failsduring the analysis you have to start again.The Cary software stores data as it isacquired, so even if someone accidentlypulls the power plug, you’ll still have thedata up to that point.


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What functionality does Cary offer forkinetics measurements? Part of the Cary Bio package is the Kineticsapplication. This application is ideal fordetermining the rates of reactions andenzyme activity.

Can I vary the data collection rate? If you have a reaction which starts off very fast and then slows you may want tocollect data at different rates over thecourse of the reaction—fast at the start (up to 30 data points per second) and thenslower during the later part of the reaction.To do this, simply specify multiple datacollection rates for different time segmentsof the assay.

The Kinetics software also caters for long,slow reactions and is capable of collectingdata for up to 8000 minutes without therestriction of a limited number of datapoints in a file.

Need an extension? If you decide during an assay that you needto change the end time, you can extend thelength of the assay without stopping themeasurement. By using the Pause functionprior to opening the sample compartmentlid to add reagents, and using the Continuefunction once the lid is closed, you can alsoprevent spikes in your data.

You can collect yourkinetics data andperform enzyme kinetics calculations all in the sameapplication. The following plots are available:Lineweaver–Burk,Eadie–Hofstee,Hanes–Woolf,Eadie–Scatchard, V0 vs [S], Dixon 1/V0 vs [I]. (Top.)

The Rapid Mixaccessory allows youto automatically start an analysis inless than 1/10th of a second after thetwo components are mixed. (Middle.)

Why do 12 experimentswhen you can do 1? The Dwell time optionallows you to measuremultiple data points per cell before movingto the next cell. Note how many datapoints were acquired by using the Dwell time feature—the insertis a zoomed sectionshowing data pointscollected over 0.015 min. (Bottom.)

Cary Bio—the master of kinetics analysis Cary Bio—unriva

With a mouse-click you can obtain akinetics curve from aseries of repetitivecurves. The insert showsthe kinetic curve at410nm. (Above.)


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Thermal denaturation/renaturationmeasurements The Cary Bio system includes the Thermalapplication software. Designed for DNAthermal melting experiments, it offers a number of unique features.

Temperature control The Temperature Controller accessoryconnects to the electrothermal multicellholder and allows temperature ramp rates as slow as 0.06 °C/min to be selected for high resolution DNA thermalmelting experients.

You can define up to 20 differenttemperature ramp rates and directions inthe same data file. This ensures the highestpossible accuracy for determining thethermal melting temperature by slowing the ramp rate and thus reducing the datainterval close to the predicted thermal melt temperature. You can even specify the temperature you want your samplesheld at after the run has finished,eliminating the possibility of destroyingthem if they are held at high temperatures.For the ultimate in temperature control, use the Cary temperature probe accesory to obtain temperature data from directlyinside cuvette.

Comprehensive built-in calculationsThe Thermal application software allows you to calculate the thermal melttemperature by either the first derivativemethod or the alpha method. Calculationsfor thermodynamic parameters for ∆S, ∆H,K at Tm , ∆G and K at user definedtemperatures are also available.

You can select up to 20 thermaldenaturation andrenaturation rampswithin one data file.This means you can doannealing and thermalmelt experiments all in one vessel.

Cary Bio—unrivalled temperature control

With the excellentsignal to noiseperformance of theCary instruments, you can detect verysmall absorbancechanges in Tm curveswith ease.


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What about concentrationmeasurements? The Cary Conc package is available for theCary 100, 300 and 400 instruments. It contains the following software:

• Simple Reads module • Advanced Reads module• Concentration module • Scanning module with Maths mode• RNA/DNA Estimation module• Instrument Validate module• GLP module for file security• Applications Development Language (ADL)

The system is ideal for Quality control laboratories and those who performquantitative measurements, with a requirement for the occasionalwavelength scan.

Up to 30 standards and 500 samplesCatering for up to 30 standards and up to5 replicates (multiple readings on the samealiquot) you have maximum flexibility interms of the level of precision you want inyour results. The built-in weight and volumecorrection will give you the final resultwithout your chemists having to performadditional calculations.

Fibre optic measurement concentrationThe Remote Read Fibre optic probe enablesyou to dip a measuring probe into anyvessel and initiate the reading from thebuilt-in switch in probe. By rinsing betweensamples the carryover is non-existentand sample throughput is increasedconsiderably. Also, you don't have to wastetime transferring samples to theinstrument cuvette.

Unattended operation If unattended automation is important then the unique Fibre Optics SPS-5Autosampler* may be the solution. It canprovide faster analysis times than arepossible with typical autosamplers as thereis no time taken for the sample to bepumped into a flowcell, and no waiting timefor the cell contents to equilibrate. It alsoeliminates the solution pumping problemsinherent in flowcell systems.

* Patent pending

The Fibre opticautosampler—fast and accurate

Cary Conc—Quantitive Analysis made easy

Making compliavalidation easy


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What about testing the performance of a Cary instrument?The Cary instruments are equipped with a range of tools to make instrument testing easy. Supplied with each package isthe Instrument Validate module. This software automates the testing of theinstrument hardware*.

GLP Compliance and Validation The Cary software is Good LaboratoryPractise (GLP) compliant*. If you need tovalidate your Cary system, Validationdocumentation is available for Caryinstruments, software and accessories.Varian, Inc. service organisations around the world support validation of ourinstruments in a number of ways, includingtraining programs, support agreements,hotlines, Telediagnostics, service contractsand certification. An overview of theValidation documentation and servicesVarian, Inc. provides is available from yourlocal Varian, Inc. office.

Can I get my Instrument recertified? At installation your instrument will bechecked against specifications. As part ofyour ongoing validation program you maywant to have your instrument recertified, to ensure that it is still meeting thosespecifications. Varian offers a recertificationservice which involves an on-site visit froma Varian Engineer who is equipped withvarious traceable standards and other testequipment. This means that you don’t haveto purchase and maintain expensivestandard materials and if the instrumentneeds adjustment, the engineer will fix it for you.

SafetyIt is Varian’s policy to manufacture safeproducts and to meet all legal requirements governing the design,manufacture and sale of safe products. As with all similar products, some or all of the following hazards may be present:high temperatures, high pressure gases,explosive gases, magnetic and radiofrequency radiation, UV and visible lightand electricity. Each product is designed toprotect operators from potential hazards.Varian supplies instructions which describethe correct procedures for the operationand maintenance of each product.

Cary ultra-violet, visible, near infraredspectrophotometers are designed tomeasure the absorption of, the reflection off and the transmission through materials.

Varian’s well established global network of subsidiaries and representatives offerscomplete Sales, Service, Training andApplications supportfor our range ofscientific instruments.

* For details refer to the separateSpecificationsbrochure.

The Validate modulemakes it easy to comply with regulatoryrequirements. Simply select which set of tests you wish to use, depending upon which regulatoryagencies you aregoverned by, e.g. European or U.S.Pharmacopeia.

Varian offers arecertification servicewhich certifies thatyour instrument isworking correctly. The Varian engineer is equipped with a large kit of certified standard materials

andothertools.

The Cary Series iscertified to complywith the requirementsof the EMC and LV directives of theEuropean Union.

Making compliance and validation easy


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What is the modular accessory concept?Each Cary accessory is built to provide afundamental requirement and can be usedwith any other accessory which requiresthat functionality. For example, severalaccessories are thermostattable, but insteadof building temperature control into eachaccessory, a single temperature controllerplugs into the thermostattable accessories.This is the concept of modular design.

How does this benefit me?By only having to buy a fundamentalrequirement once, you will save money. For example, you may want to measuremultiple cuvettes in an automated analysisas well as do gel scans. The fundamentalrequirement of moving something in thesample compartment is achieved with theSample Transport accessory. So instead ofhaving to buy a multicell holder with abuilt-in translation stage and a gel boatwith a built-in translation stage, you simplyput the appropriate accessory on top of theSample Transport.

I have built my own accessories, can I use them in a Cary?All accessories are centrally controlled bythe Accessory Controller, built into the Caryinstruments. Instead of each accessoryhaving their own electronics, the AccessoryController provides the interface betweenthe Cary software and the accessories. If your custom-built accessories will fit intoa Cary, you can use the Accessory Controllerto control them. Even external accessories,such as titrators, lasers and pH meters canbe controlled.

Fixed Angle relativeSpecular ReflectanceAccessories. Availablein 12.5°, 30°, 45°, 60°

Solid Sample Holders

Brewster Angle Holder Depolarizer and Polarizer

The modular accessory concept

Cary 400/500 LargeSample SpecularReflectance Accessory

Cary 400/500 VariableAngle SpecularReflectance Accessory

Labsphere DRA-CA-30

Cary 400/500 InternalDiffuse ReflectanceAccessory

Labsphere DRA-CA-5500A 0/45° Accessoryfor Cary 400 & 500

Labsphere DRA-CA-30IDiffuse ReflectanceAccessory

Labsphere DRA-CA-5500 DiffuseReflectance Accessory

Cary 400/500 PrayingMantis Diffuse Accessory

Labsphere DRA-CA-50MBiconical Accessory

Cary 400/500 SpecularReflectance Accessory

Cary 100/300 SpecularReflectance Accessory


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6x6 Multicell Holder–peltier thermostattable

Routine SamplerAccessory-peltier

PCB-150 Peltier Water Bath

Temperature probe

Rear Beam Attenuator Cary 100/300 8x6Multicell Holderambient/water/

Temperature Controller

Peltier1x1 thermostattedcell holder

Cell Holders

Sipper

Instrument Testing Kits

Cary 400/500 Fibre Optics Multiplexer

Total FluroescenceAccessory

Rapid Mix Accessory

Cary 100/300 Fibre Optics Coupler

SPS-5 AutosamplerSample Transport fittedwith the Film Holder

Double Aperture KitCary 100/300 ExtendedSample Compartment


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Specifications subject tochange without notice.

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CARY 100 Brochure