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Narrow Sized Dual-functional Microcapsules:
Contact Infection Control and Drug Delivery
Carl Wei He (presenter) Dr. Song Liu
University of Manitoba
Contents
Background Concepts of Microcapsules Applications (esp. in textiles)
Antimicrobial polyurea microcapsules Objective: provide contact wound dressings; Design: Feature and reasoning of the method
Details and characterizations Shell thickness Drug release Contact antimicrobial property
Future research
Advances in polyurea microcapsules
medicine
engineering
Traditional applications
Polyurea microcapsules(PUMC)
Electronic ink
Water
treatment
Self-healing
resinTextiles
Agricultures
Artificial blood cells
Targeted/Triggered release
Multi- functional carrier
Application in textiles
Cosmetic textiles
Aromatherapy & Fragrance Textiles
Thermochromic & Photochromic
textiles
Pest repellent textiles
Thermo regulating textiles
Flame retardant textiles
Objective:Antimicrobial microcapsules
Dual functions: On-contact antimicrobial property
To provide an active shell surface Controlled drug release property
To control the thickness of shell
Application Chronic wound care dressing:
infection control and nutrition supply
Requirement for the capusles Morphology:
controlled size and size distribution
Shell: matrix property: Stiff enough to retain spherical shape Thick enough to provide mass regulation
Shell: Surface property: Easily dispersed in water for application Contact-killing effect
Modification by incorporating a multifunctional surfactant
▪ Reduce interface tension▪ Provide antimicrobial functionality▪ Covalently bound to the matrix▪ Increase shell thickness
QAs
Strategy of synthesis functional surfactant
Comparison of two methods model capsules
MC+Q MCQ100a
b
c d
e
fCross-section of fluorescein stained model capsules; left: post-modified model capsule; right: in-situ modified model capsule; (a)(d) freshly prepared model capsule; (b)(e)cracked model capsules after CTAC extraction; (c)(f) close-up of fluorescein stained shell
7.00E-08
1.29E-07
4.99E-07
0.00E+00
1.00E-07
2.00E-07
3.00E-07
4.00E-07
5.00E-07
6.00E-07
Control MCQ100 MC+Q
QAs
den
sity
mol
/cm
2
Model capsules washed against Milli-Q water Before/after CTAC extraction
Before extraction
After extraction
Quantification of QAs density of model capsules. Error bar is generated from 3 batch-to-batch measurements. Control: PUMC model capsule; MCQ100: in-situ modified model capsule; MC+Q: post-modified model capsule;
Up: in-situ modified polyurea microcapsules (MCQ) Down: control polyurea microcapsules (PUMC).
(a) MCQ dispersed in water;
(b) dry MCQ;
(c) PUMC dispersed in water;
(d) dry PUMC
Comparison of two methods microcapsules
In-vitro dissolution test
7-day in-vitro cumulative drug release profile for MCQ-Cmr; insert: first 24hr release curve; (drug load = 3.33 wt%; efficiency = 31%)
Antimicrobial test diffusion
c
ba
MCQ
Control Blank
(a): MCQ, Qas modified capsule;(b): Control, Polyurea capsule without modification;(c): Blank, last batch of washing solution of MCQ sample.
Bacteria load: 4.54 log10 cfu/cm2;Capsule density: 0.88 mg/cm2
Antimicrobial test static contact
0.22 0.44 0.66 0.88
Density of microcapsules mg /cm2
MCQ
Control
Bacteria load: 1.54 log10 cfu /cm2
Antimicrobial test static contact
0.22 0.44 0.66 0.88
Density of microcapsules mg /cm2
MCQ
Control
Bacteria load: 3.54 log10 cfu /cm2
Conclusion
Isocyanate residue on polyurea microcapsules can be utilized for functionalization.
On-contact antimicrobial can be achieved on polyurea microcapsules
Sustained drug release of drug is achieved by thick shell microcapsules.
Polyurea microcapsule of narrow size distribution can be synthesized
Future research platform development
Platform for functionalzation Other functional surfactants could be
developted
Future research size control
0 1 2 3 4 5 6 7 8 9 100%
5%
10%
15%
20%
25%
30% diameter distri-bution by volumn
diameter /um
volu
mn d
istr
ibuti
on
rati
o
Acknowledgements
Manitoba Health Research Council (MHRC) Operating and Establishment grants
Dr. Paul H.T. Thorlakson Foundation FundUniversity of Manitoba Research Grant Program (URGP)
Dr. Xiaochen GuDr. Lingdong LiDr. Rick Holley Dr. Saqer Herzallah
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