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This was a small presentation I made to the class on Capillary electrophoresis. The system shown here is the one I used at Therapeutic Drug Monitoring Center - Sion Mumbai. It is a auto timed presentation where slides and points keep changing in the background at preset timings in Sync with my talk. I know that without the talk this slide show may not be that useful but still I thought of sharing it with you guys. Hope you can make something out of it.
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CAPILLARY ELECTROPHORESIS
BASIC CONCEPTS & HARDWARE
by Sunil Budke
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CE by SUNIL BUDKE 04/08/23 11:16
Source vial
Destination Vial
Capillary tube
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Electropherogram from CE
CAPILLARY Made of fused silica Polyamide coating for protection Pyrex or Teflon capillaries are available Effective length 10 - 100 cm Internal diameter (ID) 20 -100 micro meter C8 and C18 coated capillaries are
available
SAMPLE INTRODUCTION
Electrokinetic Injection5kv for 5 – 10 sec
Gravimetric (Syphonic)Sample vial held at higher level
Differential pressurePressure applied on sample vial or vacuum applied on
Destination vial
DETECTORS Conductivity Detectors
UV Detectors
Fluorescence Detector
Electrochemical Detector
Mass Spectrometer
CE by SUNIL BUDKE 04/08/23 11:16
DISCUSSION
References Capillary Electrophoresis
-by Dale R. Baker
Capillary Electrophoresis for Food Analysis – Method Development
-by Richard A. Frazier; Jennifer M. Ames and Harry E. Nursten
Principles and Practice of Bioanalysis -by Richard F. Venn
McGraw Hill - Year book of Science and Technology – 2002-Capillary electrophoresis
Thank You
