ANALYTICALSOLUTIONSA U S T R A L I A

Cannabinoid Analytical and Preparative Separations

Supercritical Fluid Chromatography (SFC) is a relatively new technique that offers several advantages including faster run times, better resolution and more concentrated separations.The limitations of SFC are: it has only been proven on small molecular groups and gradient development is more complicated than for HPLC. The cannabinoid group is one application that works well on SFC. The main cannabinoid species that require separation are Cannabidivarin, Cannabidiol, Cannabichromene, Tetrahydrocannabivarin, Tetrahydrocannabinol,Cannabinol, Cannabigerol, Cannabidiolic acid, Tetrahydrocannabinolic acid and Cannabigerolic acid.

Column type:

Eluent:

Flow rate:

Oven °C:

Back P:

Detection:

Phenomenex Lux, 5µm

CO and co-solvent Ethanol

5ml/min

24

100 bar

DAD @ 220nm

2

ANALYTICAL SEPARATION OF CANNABINOIDS BY SFC

Separation conditions: Cannabinoids Chemical Structures

1. Cannabidivarin (CBDV)2. Cannabidiol (CBD)3. Cannabichromene (CBC)4. Tetrahydrocannabivarin (THCV)5. Tetrahydrocannabinol (THC)6. Cannabinol (CBN)7. Cannabigerol (CBG)8. Cannadiolic Acid (CBDA)9. Tetrahydrocannabinolic Acid (THCA)10. Cannabigerolic Acid (CBGA)

6

Time (min)

Nor

mal

ised

resp

onse

(220

nm)

45

3

21

7 89

10

1. Cannabidivarin (CBDV)

3. Cannabichromene (CBC)

5. Tetrahydrocannabinol (THC)

7. Cannabigerol (CBG)

9. Tetrahydrocannabinolic acid (THCA)

2. Cannabidiol (CBD)

4. Tetrahydrocannabivarin (THCV)

6. Cannabinol (CBN)

8. Cannabidiolic acid (CBDA)

10. Cannabigerolic acid (CBGA)