Cancer Immunotherapy

Using xCELLigence® Instruments to Monitor Immune Cell Killing in Real-Time

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Is Your In Vitro Potency Assay Physiologically Relevant?

LABEL-FREE: No 51Cr, no luciferase, no dyes…NO PROBLEM

EXQUISITE SENSITIVITY: Monitor target cell killing at low, physiologically relevant effector:target ratios

ASTONISHINGLY SIMPLE WORKFLOW: Plate target cells, add effector cells, and start reading

QUANTITATIVE, REAL-TIME KINETICS: Measure immunotherapy-specific parameters such as %cytolysis and KT50 with high accuracy and reproducibility

Now it can be. For diverse immunotherapy applications reap the benefits of xCELLigence® Real-Time Cell Analysis (RTCA) instruments:

Easily Study Diverse Effector Cells and Molecules:

- Read an entire 96-well plate in 7 seconds- Run up to 6 plates independently, with no scheduling conflicts- Monitor target cell killing continuously from seconds to days

xCELLigence Instruments for Cancer Immunotherapy Assays:Single Plate

1x96 wells

Multi Plate

6x96 wells3x16 wells

Dual Purpose High Throughput

Up to 4x384 wells

The information-rich killing kinetics of xCELLigence assays provide substantial insights within diverse immunotherapy applications, including everything from validating neoantigen targets and optimizing the signaling domains of chimeric antigen receptors to comparing the efficacy of checkpoint inhibitors and developing combination therapies.

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How is xCELLigence RTCA Used for Cell Killing Assays?The xCELLigence Real-Time Cell Analysis (RTCA) instruments utilize patented microtiter plates (E-Plates®) which contain gold biosensors embedded in the bottom of each well. These biosensors continuously and non-invasively monitor changes in cell number, cell size, and cell-substrate attachment quality.

Step 1: Adherent target cells (i.e. tumor cells) are first seeded in E-Plate® wells. The biosensor signal, also known as “Cell Index”, increases as cells attach and proliferate and then plateaus as cells approach 100% confluence (see data plot below).

Step 2: When added subsequently, non-adherent effector cells (i.e. immune cells) in suspension do not cause signal changes in and of themselves (due to lack of adherence to the gold biosensors).

Step 3: If effector cells induce the destruction of the target adherent tumor cells, the corresponding cytolytic activity can be sensitively and precisely detected. The continuous acquisition of data for each well of an E-Plate enables the generation of real-time killing curves for multiple conditions simultaneously.

Step 4: Using an intuitive graphical interface designed specifically for the immuno-oncologist, the xIMT Software monitors cell killing in real-time (left panel), followed by calculation of specific parameters such as %cytolysis and KT50 (right panel), IC50, etc.

Learn more:Download an xCELLigence RTCA Immunotherapy Handbook, search xCELLigence publications, and see example data at: www.aceabio.com/applications/cancer-immunotherapy/

Dead Target Cells

Adherent Target Cells Microelectrodes

+ Non-adherentEffector Cells

Data Plotting& Analysis

Trademarks:xCELLigence®, E-PLATE® and ACEA BIOSCIENCES are registered trademarks of ACEA Biosciences, Inc. in the U.S. and other countries.All other product names and trademarks are the property of their re-spective owners.

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Published by:ACEA Biosciences, Inc.6779 Mesa Ridge Road Ste. 100San Diego, CA 92121U.S.A.