(c) Process Analysis & Automation Ltd. 2005

© Process Analysis & Automation Ltd. 2005

Introduction

• OVERLORD™• Labcyte Echo 550 Nanolitre Dispenser• Extending functionality using OVERLORD™• Integrating the Echo into an automated system

© Process Analysis & Automation Ltd. 2005

OVERLORD™

• PAA offer two software products to automate Echo 550• OVERLORD™ Workstation• OVERLORD™ Scheduler

© Process Analysis & Automation Ltd. 2005

OVERLORD™ Workstation

• Icon based drag and drop set-up• Flow chart interface • Very flexible• Dynamic Scheduling• Decisions can be made in real-time

© Process Analysis & Automation Ltd. 2005

OVERLORD™ Scheduler

• Icon based drag and drop set-up • Gantt chart interface • Optimise/Interleave assays in single run• Each assay run with exact timings• Very easy to create an assay• Add new assays when system is running

© Process Analysis & Automation Ltd. 2005

Common Features

• Support for over 200 instruments• Uses original instrument software when possible• Automatic data handling• Can integrate with most databases

(e.g. SQL Server, Oracle, Microsoft Access etc)• Optional 21 CFR Part 11 compliance• Full system tracking• Error notification• Error recovery

© Process Analysis & Automation Ltd. 2005

Labcyte Echo 550

• Transfers volumes down to 2.5nl• Uses “touchless” technology • Based on focused acoustic energy• Can measure the water content of

DMSO and the fluid depth in each well

© Process Analysis & Automation Ltd. 2005

Labcyte Echo 550 Functionality

• Plate to Plate Transfers (Quadrant or Interleaved)• Plate Surveys

• Use 384 or 1536 well plates• Protocols defined in the Client software• Data reported in XML

© Process Analysis & Automation Ltd. 2005

Functionality with OVERLORD™

• Plate to Plate Transfer - Interleaved or Quadrant• Plate Survey – Includes reporting sample volume• Cherry Picking software module available• IC50 software module available

• Use 384/1536 plates OR 96 well destination plates• Protocols defined in OVERLORD™• Data reported in any format

(CSV, Excel, XML, Write to SQL Server, Oracle, etc)

© Process Analysis & Automation Ltd. 2005

Integrating into a Workcell

• Labcyte Echo 550 can be integrated with nearly any device

• Robotic arms such as the automate.it KiNEDx arm can be used

• All fully controlled from OVERLORD™

© Process Analysis & Automation Ltd. 2005

Summary

• OVERLORD™ Workstation and Scheduler• Functionally of Labcyte Echo 550• How OVERLORD™ can increase the functionality of

the Labcyte Echo 550• How OVERLORD™ can integrate the Labcyte Echo

550 into an automated system with other equipment

© Process Analysis & Automation Ltd. 2005

Any other questions / points?

All trademarks acknowledged