Slide 2 By: Zachary Bryan Scott Department of Civil and
Environmental Engineering University of California, Irvine Slide 3
Motivation Wastewater Treatment: Aerobic Biological Treatment of
Wastewater Cleans wastewaters Generates Biomass (0.4g VSS/ g COD)
Anaerobic Biological Treatment Cleans wastewaters Many Types
Reduces biomass (0.05 g VSS/ g COD) Can produce net energy Consider
heating, transport, 4 ft 3 CH 4 / lb VS as manure 2 Slide 4
Potential Uses of Process Models: 3 Reactor Assessment/Optimization
Design Process Modification Slide 5 Do Current Process Models Make
Predictions of Sufficient Accuracy? 4 Modify Reactor Conditions:
pH, T, Feed Type, Feed interval, Mixing Intensity, Nutrients
Current Industrial Use of Models is very limited Pilot testing
still dominant = higher costs = more time Slide 6 5 Modified
figure, Gujer and Zhender, 1979 Anaerobic Process: Slide 7 Three
Critical Measurements: Methane Production Rates- Flowcell Acetate
Consumption Rates-GC Aceticlastic Methanogen Ribosomal DNA Copy # -
qPCR Slide 8 6 Batch Reactors Highly Degraded Manure-Media (MSL)
Fed Acetic Acid Acrylic, Rod Mixed Glass, Shaker-Mixed Slide 9
Acrylic, Rod Mixed Slide 10 Glass, Rod-Mixed Slide 11 How are
Organisms Identified and Quantified in mixed culture? Each
taxonomic group has ribosomal DNA (rDNA) that is unique to its
group This information is available in public databases. ex: NCBI
rDNA is extracted from samples quantitative Polymerase Chain
Reaction assays quantify the DNA Technique requires prior knowledge
of organisms genome Probes and primers complement and bind to
target DNA 10 Slide 12 Methods Organism Quantification 11 1.Collect
Sample 2.Extract and Purify DNA 3.Quantify the DNA of important
anaerobes Slide 13 Releasing DNA The key to good analysis is the
total release of DNA from the cell. Use glass beads to beat cells
in order to break the cell wall and membrane. DNA Needs to be
released by beating with glass beads 12 Olson, UCI, CEE 263, 2008
Slide 14 Flowcell Basic Principles First Cell Measures Total Gas,
Second Cell Measures Methane Glass scrubber with 2 M NaOH to remove
CO 2., Bromocresol Green Indicator, color transition, clear to
green at pH 12 Slide 15 R1R2 - 5 V + Two Resistors in Series R1:
12k , R2:Cd_S(1-50)k 15 mC LED & 200 R Gas inGas Out FLOW- CELL
- + Volts Slide 16 Flowcell Wiring Slide 17 2 Cell Circuit Diagram,
Plan View Slide 18 Slide 19 Biogas Chromatogram, GC Slide 20
Scrubbed Biogas, no CO 2 Slide 21 Plumping and Circuit Diagram, 6
Reactors Physical connections to Match Software Organization
R1R3R4R2R5R6 1234567812345678 USB Device PC
