Buch DASware migrate en - User Manual Search …...DASware migrate integrates most common 3rd party bioreactor control units and systems (e.g. Sartorius Stedim Biotech & Applikon)

e-Bedienungsanleitung073DE)e-Bedienungsanleitung

DASware® migrate

78000153/032013

Copyright© 2012-2013 DASGIP Information and Process Technology GmbH. No part of this publication may be reproduced without the prior permission of the copyright owner.

DASGIP Information and Process Technology GmbHRudolf-Schulten-Straße 552428 JülichGermanyFon: +49 (0)2461 / 980-0Fax: +49 (0)2461 / 980-100www.dasgip.comEmail: [email protected]

Trademarks

Eppendorf® and Eppendorf Logo are registered trademarks of Eppendorf AG.

DASGIP® and DASware® are registered trademarks of DASGIP Information and Process Technology GmbH, Germany.

CelliGen®BLU, BioFlo® and BioCommand® are registered trademarks of New Brunswick Sientific Co., Inc., USA.

800xATM is a trademark of ABB.AberTM is a trademark of Aber Instruments Ltd.Applikon® ADI 1010 and BioXpert® are registered trademarks of Applikon B.V.BaychroMAT® is a registered trademark of Bayer Technology and Services.BIOSTAT® is a registered trademark of Sartorius AG.DeltaVTMis a trademark of Emerson Process Managment.MatrikonOPCTM is a trademark of Matikon Inc.OSIsoft® and PI System® are registered trademarks of OSIsoft, LLC.YSITM is a tradmark of Xylem Inc.

Registered trademarks are not marked in all cases with ® in this manual.

Table of Content

1 User Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31.1 Using this Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31.2 Material Damages Due to Incorrect Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

1.2.1 Danger Symbols and Danger Levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31.3 Common Format Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41.4 Abbreviations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41.5 Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

2 DASware® Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52.1 DASware® access . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.2 DASware® analyze . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82.3 DASware® connect . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92.4 DASware® design . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102.5 DASware® discover . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112.6 DASware® migrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3 Product Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133.1 Main Illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143.2 Delivery Package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153.3 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153.4 Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

4 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 174.1 Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

4.1.1 Warning for Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 174.2 Information on Product Liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195.1 Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

5.1.1 New Brunswick . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195.1.2 Sartorius DCU . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 205.1.3 Applikon ADI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 205.1.4 IT Network Integration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 236.1 First Steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

6.1.1 Login . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 236.2 Overview of Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

6.2.1 Settings for Bioprocess Controllers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256.2.2 Functional Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 266.2.3 Overview of Operating Sequences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

7 Quick Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 437.1 Short Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 437.2 Calibration and Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 437.3 Start a New Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 437.4 Recipe Editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 477.5 Process Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 487.6 Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

7.6.1 Data Export . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 507.6.2 DASGIP Documentation Wizard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

8 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 618.1 Error Search . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

8.1.1 Bioprocess controller is not accessible . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

3User InstructionsDASware® migrate

English (EN)

1 User Instructions

This User Manual includes special details regarding to DASware migrate and the handling of 3rd partybioprocess controllers with DASGIP Control. Refer to the DASGIP Control User Manual for complementaryinformation.

1.1 Using this Manual

1.1.1 Material Damages Due to Incorrect Use

• Only use the product for its intended purpose as described in the User Manual.• Ensure adequate material resistance when using chemical substances.• In case of doubt, contact the product manufacturer.

• Before using the DASware migrate for the first time, please read the User Manual.• Please view this manual as part of the product and keep it somewhere easily accessible.• When passing on the software DASware migrate always enclose the User Manual.• If the manual is lost, please request another one.

1.2 Danger Symbols and Danger Levels

DangerRisk of electric shock with potential for severe injury or death as a consequence.

NoteRefers to particularly useful information and tips.

User InstructionsDASware® migrateEnglish (EN)

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1.3 Common Format Conventions

Tab. 1: Format Conventions

1.4 Abbreviations

Tab. 2: Abbreviations

1.5 Glossary

Tab. 3: Definitions

Representation Meaning

You are requested to perform an action.

1.2.

Perform these actions in the sequence described.

• List

Text Text

Text Terms and key names from the software.

Abbreviation Description

DO Dissolved Oxygen

DoE Design of Experiment

HPLC High-Performance Liquid Chromatography

NB New Brunswick

.PV Process Value

.SP Set-Point

Term Meaning

Comment Additional descriptions that can be inserted

Event Monitor The lower part of the DASGIP Control window, which shows all events chronologically and color coded.

Inoculation Addition of precultured cells or bacteria to the medium for cultivation

Login, Logout Sign in, out for the program

Set-point The target value that the controller or the control system will aim to reach.

Tools Induces more options

Workflow Sequence of process steps

5DASware® Solutions

DASware® migrateEnglish (EN)

1 DASware® Solutions

Increasing process complexity coupled with rising cost pressures and rapidly evolving regulatoryrequirements make today’s process development efforts a special challenge. The pressure of achievingfaster time to market for new and innovative biopharmaceuticals has led to the need to optimize everyelement of the total development workflow.

DASGIP‘s DASware was designed as a suite of smart and flexible software solutions to acceleratebioprocess development. It enables interconnectivity of bioreactors with external lab devices,comprehensive data- and information management, factorial design of experiment and remote control ofbioprocesses. DASware can be used with the DASGIP Parallel Bioreactor System for benchtop applicationsin microbiology and cell culture as well as with legacy benchtop bioreactor controllers.

In the past, bioreactor processing, recipe management, process and product analysis, data generation, datamining and storage were poorly interconnected thus requiring time-intensive manual work. DASwaresupports interconnectivity combined with sophisticated bioprocess information management. Theutilization of DASware enables streamlined process development at benchtop scale in accordance with QbDstandards.

DASware opens up additional dimensions for bioprocesses and their information management. Usersbenefit from accelerated up workflows, which e.g. are also DoE capable, optimal interconnectivity betweenbioprocess-controller and analyser as well as almost unlimited access to bioprocesses.

DASware is designed for…

• ensuring an extensive information management

• meeting FDA’s QbD and PAT guidelines

• assigning DoE concepts for bioprocesses

• integrating third-party analyzer, bioprocess controller and software tools

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DASware constists of…

DASware accessfor remote monitoring and controlling of bioprocesses

DASware analyze

for integration of lab devices with DASGIP Parallel Bioreactor Systems

DASware connect

facilitates a seamless interaction with legacy Corporate Historians and Process Controlllers

DASware designto apply the Design of Experiments (DoE) concept

DASware discovera comprehensive and user-friendly data mining and information management solution forbioprocessing

DASware migrateto use all DASware solutions with legacy benchtop bioreactor control units



1.1 DASware® access

DASware access provides an unprecedented level of freedom and flexibility in the management ofbioprocesses. Each DASGIP Control System on-site is accessible remotely by one or more remote clientssimultaneously. Depending on the user-defined configuration and associated authentication either monitoror monitor and control access can be enabled for any network or mobile client.

Wi-Fi, Intranet, VPN and 4G connections can be used to provide web based access with almost everybrowser to one or more bioreactor systems via PC, Notebook or Netbook. The unique DASGIP iAppsupports access from iPhone, iPod touch and iPad, optionally with webcam support.

DASware® access

DASGIP® iApp


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1.2 DASware® analyze

DASware analyze was designed for seamless integration of sampling and analytical laboratory devices tothe bioreactor system. A broad and growing range of analyzers can be integrated, among them nutrientanalyzers, cell counter (YSI Life Science, Nova Biomedical), automation platform and autosampler (BayerTechnology Services, BaychroMAT®) biomass monitors (Aber Instruments), HPLC (Waters, Shimadzu) andmass spectrometers (Thermo Scientific).

The OPC network protocol allows for interconnectivity between the bioreactor system and the analyzer,including the possibility of direct feedback from the bioreactor system according to online measuredanalytical data. This facilitates feedback control loops for e.g. nutrients, biomass or product concentrations.Online calculations as well as event and data-driven decisions are supported. The unique bidirectional OPCcommunication, available for supporting devices enables sampling on demand and process dependentanalyzer panel selection.



1.3 DASware® connect

DASware connect was designed to integrate DASGIP’s Parallel Bioreactor Systems and 3rd partybioprocess controllers into process control systems and legacy corporate historians. This includes but isnot limited to

• Emerson DeltaVTM

• Siemens SIMATIC PCS 7

• ABB 800xATM

• OSIsoft® PI System®

• Matrikon OPCTM Historian

DASware connect facilitates company-wide access to all relevant bioprocess data like set-points, processvalues, feed-profiles, calibration and controller parameters as well as events and alarms.


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1.4 DASware® design

DASGIP’s Parallel Bioreactor Systems serve as an ideal platform to carry out the Design of Experiment(DoE) principle on bioreactors in parallel. DASware design automatically compiles DoE information from3rd party DoE tools into recipes and feeds-back response information into DoE and multivariate analysisand reporting tools.

DASware design comes with a built-in Full Factorial DoE builder. Alternatively, a large variety of DoEdesigns for screening, process development and optimization can be automatically imported from mostpowerful 3rd party DoE tools like JMP and others. Parallel recipes incorporating the DoE factor variations(i.e. pH, DO, T set-points or feed rates) are automatically populated. Following DASGIP’s Point-Click-Growconcept, they can be carried out on a set of bioreactors with a single mouse-click. DoE responseinformation is collected and prepared for an automated export.



1.5 DASware® discover

DASware discover helps you to structure efficiently the assortment of process information like

• Process values,

• Process events,

• Recipes,

• Biological,

• Chemical and

• Analytical information

Use this intuitive database query tool to initiate real-time retrievals and run-to-run comparisons. All criticalprocess information is clearly listed as table and can be configured. In addition, you can easily generatecharts in Microsoft Excel® using DASGIP Documentation Wizard.


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1.6 DASware® migrate

A significant installed base of ageing bioreactor controllers offers rudimentary cultivation controlcapabilities. However, recent advances such as bioprocess data management, analytical device integration,DoE support and remote control, available from DASware solutions, are often missing. Now users of 3rdparty bioreactor controllers can also benefit from DASGIP’s intelligent software solutions.

DASware migrate integrates most common 3rd party bioreactor control units and systems (e.g. SartoriusStedim Biotech & Applikon) as well as DASGIP modules into legacy bioreactor controllers (e.g. gas analyzerGA4, gassing station MX4/4, mulitipump MP8).

DASware migrate enables

• access to the complete DASware solution suite

• sharing recipes between different bioprocess controllers

• powerful Microsoft Excel® reporting

• comprehensive information management and process data historian

• analytical lab device integration

• DoE and remote control

13Product Description


2 Product Description

DASware migrate combines the advantages of communication and information management achieved byDASGIP Control with widely used bioprocess controllers. Use the intuitive user interface to optimize yourcell cultivation or fermentation processes by parallelism. Increase the functionality of your existing systemby the integrated reporting and process documentation of DASGIP Control. It provides an easy recipemanagement and improves the effectiveness of workflows by automation.

Product DescriptionDASware® migrateEnglish (EN)

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2.1 Main Illustration

Fig. 2-1: Overview of DASware migrate

15Product Description


2.2 Delivery Package

• DASware migrate License

• DASGIP Process Computer, if not yet existing.

2.3 Features

The open connectivity of DASGIP Control featuring DASware migrate unifies communication, capture andcontrol. This provides the potential to increase the performance of a lab in combination with 3rd partybioprocess controllers effectively.

DASware migrate provides:

• Support to many third party bioprocess controllers (z.B. New Brunswick BioFlo, CelliGen 115, 310 and BLU, Sartorius DCU, Applikon ADI 1010, 1030, ez-Control), others on demand

• Seamless integration with DASGIP Control (version 4.5 and higher)

• Display, logging and charting of controller variables in DASGIP Control

• Control options for set-points using DASGIP Control

• On and off switching of the bioprocess controller using DASGIP Control

• Seamless integration of DASGIP Advanced Trigger Automation and DASGIP Advanced Scripting

• Addition of advanced feeding by highly precise speed controlled miniature pumps, mass-flow controlled gas-mixing as well as OD and off-gas DASGIP modules.

• Support to integrated one-click batch reporting and exports data to Microsoft Office® especiallyMicrosoft Excel®.

2.3.1 Requirements

• DASGIP process computer with Windows XP Professional operating system

• Network or serial connection between process computer and 3rd party controller

• DASGIP Control software installed on the process computer

• DASware migrate software installed on the process computer

• Available for New Brunswick BioFLO and CelliGEN 115, 310 and BLU, Sartorius B-DCU or Applikon 1010, 1030 and ez-Control.

• One DASware migrate license needed for each external Controller / Bioreactor

Product DescriptionDASware® migrateEnglish (EN)

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17Safety


3 Safety

3.1 Intended Use

3.2 Warning for Intended Use

Read the User Manual first and observe the following general safety instructions before using DASwaremigrate.

3.2.1 Information on Product Liability

In the following cases, the protection provided by the device may be impaired. the liability for the functionof the device passes to the operator if:

• The device is not used in accordance with the User Manual.

• The device is used outside of the range of application described in the preceding chapters.

• The owner has made unauthorized modifications to the device.

Lethal voltages inside the device.

Ensure that the housing is always closed and undamaged so that no parts inside the device can be contacted by accident.

Do not remove the housing of the device.

Do not allow any liquids to penetrate the inside of the housing.

Electric shock due to damages to device or mains cable.

Only switch on the device if the device and mains cable are undamaged.

Only use devices that have been properly installed or repaired.

In case of danger, disconnect the device from the mains supply.

SafetyDASware® migrateEnglish (EN)

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19Installation


4 Installation

Each DASGIP process computer comes pre-configured with all necessary software installed. The license ofDASware migrate requires no further installation steps. All relevant services (gateway) will startautomatically within the program start of DASGIP Control.

4.1 Interfaces

4.1.1 New Brunswick

Connect the serial port of a New Brunswick bioprocess controller to the serial port of the device server (i.e.Moxa) according to Fig. 4-1. The device server is connected to the DASGIP process computer via Ehernet.

Fig. 4-1: Connection map for a New Brunswick bioprocess controller

You can not use DASware migrate and New Brunswick BioCommand at the same time.

InstallationDASware® migrateEnglish (EN)

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4.1.2 Sartorius DCU

Sartorius bioprocess controllers can be directly connected to the DASGIP process computer via Ethernetaccording to Fig. 4-2.

Fig. 4-2: Connection map for a Sartorius bioprocess controller

4.1.3 Applikon ADI

Connect the serial port of an Applikon bioprocess controller to the serial port of the device server (i.e.Moxa). The device server is connected to the DASGIP process computer via Ethernet.

It may be necessary to involve your IT administrator to ensure proper configuration, IPaddresses and port settings.

You can not use DASware migrate and Sartorius MFCS/win at the same time.

21Installation


Fig. 4-3: Connection map for an Applikon Controller

4.2 IT Network Integration

In a local set-up the DASGIP Process Computer is directly connected to one or more Controllers usingnetwork cable or serial device servers. If the Process Computer is integrated in the local IT infrastructurethe set-up can be different to the installations described before.

You can not use DASware migrate and Applikon BioXPert at the same time.

It may be necessary to involve your IT administrator to ensure proper configuration, IPaddresses and port settings.

InstallationDASware® migrateEnglish (EN)

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23Operation


5 Operation

DASware migrate displays all relevant process and controller values as well as set-points of the integratedbioprocess controller in a Summary and Process View of DASGIP Control (s. 5.3.2).

5.1 First Steps

Switch on the process computer.

5.2 Login

The program symbol of DASGIP Control is displayed on the desktop of the process computer.

Double click on the program symbol to start DASGIP Control.

Enter your user Name and the corresponding Password.

Click Login to continue.

You can operate the bioprocess controller using the operation/touch panel of the controller orDASGIP Control. For more details regarding the process start refer to 6.1.4.

Please enable the remote function of the bioprocess controller (according to the manufacturer‘smanual) to ensure the full access to all available operations for DASGIP Control.

OperationDASware® migrateEnglish (EN)

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5.3 Overview of Operations

DASGIP Control is subdivided into three areas Fig. 5-1.

Fig. 5-1: Overview of DASGIP Control

Refer to chapter Fig. 5.3.2 and Fig. 5.3.2.1 for more details.

1 Navigation areaThis area includes three different tabs:

– Overview with Navigation Tree– Instructions – Navigation for a quick switching between

the different views

3 Event MonitorDisplays all events, interventions and error messages

2 Working AreaThis area displays the Summary and Process View

25Operation


5.3.1 Settings for Bioprocess Controllers

Since the DASware migrate license is activated, the integrated bioprocess controller is displayed in DASGIPControl. Use the following procedure to check the settings:

Choose the Overview tab on the left to display the navigation tree. Click on Plant > DASGIP Plant … > Modules to show the list of all integrated bioprocess controllers and other connected modules.

Right click on the controller name to open the context menu.

Choose Info to display the corresponding information on the right.

5.3.1.1 New Brunswick

5.3.1.2 Sartorius


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5.3.1.3 Applikon

5.3.2 Functional Overview

DASGIP Control displays for the Navigation area three different views - Overview, Instructions, andNavigation - at the left side (refer to the DASGIP Control User Manual for complementary information).

Please make sure that the IP address, which is shown as Port, matches yourcontroller and network settings.The IP address 127.0.0.1 indicates direct connection as described in chapter4.1.

27Operation


When you have started a process from the bioprocess controller, DASGIP Control displays the processname on the Overview tab under the branch Running in the Navigation Tree.

5.3.2.1 Summary View

Use the Navigation tab for a simple and intuitive operation within DASGIP Control. You can easily switchbetween Summary and Process View of different setups (vessels) of a process at any time.

Click on the Xxx.Control button to display a Summary View, which gives a summary of all process values like pH, DO value(s), gas mixture, gassing rate and feeding volumes for a set of vessels selected to be operated in parallel (Fig. 5-2, Fig. 5-3, Fig. 5-4). Large numerical display elements facilitate an easy reading of all relevant process values.

The displayed views vary depending on the integrated bioprocess controller. The following shows thedifferent Summary Views of DASGIP Control with an integrated BioFlo/CelliGen 115 from New Brunswick, aDCU from Sartorius, and an ADI 1010 from Applikon illustratively.


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New Brunswick

Fig. 5-2: Summary View for a New Brunswick bioprocess controller

Sartorius

Fig. 5-3: Summary View for a Sartorius bioprocess controller

29Operation


Applikon

Fig. 5-4: Summary View for an Applikon bioprocess controller

The operating status of different sensors and actors (feeding pumps, gasmixer, impellers, etc.) is displayedby color coded symbols and monitored. The following table (Tab 5-1) shows the color definitions for thesymbols.

Additionally, you can click on each symbol to display the current process values of all affected reactors onthe right side in the Summary View.

Tab. 5-1: Color definitions of Symbols

Symbol Definition

Green symbols indicate active elements.

Orange symbols indicate elements in manual mode or operated by the bioprocess controller.

Gray symbols indicate elements in standby mode or inactive elements.

Blue symbols indicate elements in idle mode.

Blue symbols indicate elements in error mode.

To get the full access to each vessel in the subordinated Process View later on, you have toactivate all sensor and control options in the Summary View superordinately, which means thatall symbols are displayed in green.


30

The operating status of different sensors and actors (feeding pumps, gasmixing, impellers, etc.) isdisplayed by color coded symbols and monitored. The following table (Tab 5-2) shows the color definitions.

5.3.2.1 Process View

The Process View (Fig. 5-5, Fig. 5-6, Fig. 5-7) displays all relevant process values to single setups / vessels.

Fig. 5-6:Switch to the Prozess View of a single setup by clicking on the corresponding Setup button on the Navigation tab.

When you have started a process within the bioprocess controller, Process View displays all color codedtransferred controller states.

The following shows the different Process Views of DASGIP Control with an integrated BioFlo/CelliGen 115-from New Brunswick, a DCU from Sartorius, and an ADI 1010 from Applikon illustratively.

Tab. 5-2: Color definitions of edit boxes

Gray fields indicates read-only values.

Values of white fields can be edited.

Click on a white field to edit the valueGray fields with small triangles in the right upper corner indicate values, whichshould be analyzed before changing.

Click on the triangle for editing.– Please note that this interrupts the automatic mode (gray) and switches into a

manual one (white).

31Operation


New Brunswick

Fig. 5-5: Process View for an integrated BioFlo/CelliGen® 115 controller

Sartorius

Fig. 5-6: Process View for an integrated DCU controller


32

Applikon

Fig. 5-7: Process View of integrated ADI controller

Please check and activate / deactivate each function according to your needs. DASGIP Control does notforce the controllers into a default state to avoid conflicts with local operations. For this reason the on/offsymbols (x/o) may not reflect the controllers state/s through the color does.

Synchronized display (color matches x/o symbol). Click on the cross or the white point of asymbol to activate or deactivate the corresponding action.

Unsynchronized display (color does not match x/o symbol).

Different symbols are available for vessels, sensors and controllers as well as actors likepump, gassing and agitation.

Controllers like pH and DO (i.e. DO Control) can operate actors like pumps, gas mixing or agitation.

In this case orange symbols indicate actors which are controlled interdependently.

The color indicates that direct access it is not possible. You have to inactivate the controller itselffirst by clicking on the white point of the symbol e.g. of DO Control to get access to theinterdependent gas mixing. After a short delay the gas mixing symbol will turn from orange to grayand can be operated manually.

33Operation


Click on the vessel symbol to display the vessels parameters at the right side of the working area.

Click on a controllers symbol to display the corresponding controller parameters.

The parameter dialogs are subdivided in three tabs:

• Details shows all relevant parameters at a glance. Edit boxes are displayed in white.

• Profile s. chapter 5.3.2.1.

• Alarm (optional see also Alarm Option)

Please refer to the DASGIP Control User Manual for complementary information.

The following table (Tab 5-3) lists parameter definitions.

Tab. 5-3: Parameter Definitions

Abbreviation Parameter Definition

State State The operation state of a controller is displayed as Busy, Manual,Inactive or Error.

P Proportional Value The proportional value (P-Tune) directly determines the intensity ofthe control. The higher the chosen value, the more invasive is thecontrol.


34

5.3.2.1 Define or Adapt Set-point Profiles

DASGIP Control provides a very easy to use and flexible way to enter and edit controller profiles.

Click on a controller symbol in Process View to open the corresponding values in Details at the right side.

Ti Reset time The reset time (Ti) influences the integrating part and therefore thesensitivity of the control. The larger the value, the smaller the effect onthe control. The control becomes smoother, but slower at the sametime.

Td Derivation time Differentiator

Min Lower limit Minimum value of the control variable (Anti-Reset-Windup).

Max Upper limit Maximum value of the control variable (Anti-Reset-Windup).

Deadband Deadband Control deadband for a two-sided control.

Slope Slope Sensor slope calibration parameter.

Offset Offset Axis shift as calibration parameter.

Calibration Calibration Flow rate at 100 % output.

Please note that not each 3rd party bioprocess controller supports all parameters. Therefore theDetails tab in DASGIP Control displays differing parameters depending on the integratedcontroller.

Abbreviation Parameter Definition

35Operation


Click on the Profile tab to enter and edit the corresponding profile options.

The drop down menu of Profile Source gives the opportunity to choose between different time or valuebased profiles (a detailed list of all profile sources is given in chapter 3.3.2 of the User Manual DASGIPControl).

Each profile is sectioned into the three distinct presentation modes Type, Data and Graph:

Type comprises three different profile types. Choose Constant Value, Datatable or Function as profile type.


36

Use the Data tab to enter profile values and Graph to show the corresponding profiles graphically (pleaserefer to chapter 3.3.2 and 3.7 of the User Manual DASGIP Control for more details).

5.3.2.2 Alarm Option

If the alarm option is activated, DASGIP Control displays alarm options on an Alarm tab within the specifiedsensor values separately.

Click on the title bar or a process value (e.g. DO.PV) to display the corresponding sensor values. There is an additional Alarm tab, if the alarm option is activated.

Click on the Alarm tab to adjust the alarm settings.

Please note that all alarms are inactive until the inoculation clock(Inoculation) is started.

37Operation


5.3.2.3 Offline Values

You can supplement the process documentation with offline values in DASGIP Control. These valuesdetermined by external analyzers can be entered together with the time stamp of determination directly orlater on. They will be stored as an event in the database and can be displayed together with all otherprocess values e.g. in a diagram.

Click on the Offl. symbol in the Process View to display the dialog at the right side. You can enter up to four Offline values and user events by default. Additionally, you can specify the name as well as the engineering unit. Please refer to DASGIP Control User Manual chapter 5.1 for details.

5.3.2.4 Chart Control Panel

During a process run all values are visualized in online charts in the Summary or Process View (Fig. 5-8) atthe bottom of the window.

Fig. 5-8: Process View with Chart Control Panel


38

Click on the corresponding curve button to hide or display a curve on the diagram.

Click on the chart, hold the mouse button and drag a box to zoom in an area.

Click on the x- or y-scale and drag down the appearing double line to display a legend with corresponding values.

Click on the Tools button on the upper left edge of the Chart Control Panel to display further options. You can display all available data (Show all curves) or just data of determined time intervals (Zoom to last hour‘s data).

You can configure and save chart settings individually (Save User chart settings). Please refer to the UserManual DASGIP Control chapter 6.1.1. for more details.

5.3.2.1 Event Monitor

The Event Monitor at the bottom of the DASGIP Control window displays all events, interventions andpossible error messages of the connected bioprocess controllers during the process runtime.

39Operation


The following table (Tab 5-4) shows the color code of the Event Monitor.

Tab. 5-4: Color code for the Event Monitor

5.3.2.1 Plant Overview

The Plant Overview gives a complete tabular overview of the current process status and eases the access tomain procedures’ process values. Additionally, the already logged process values of the running processcan be viewed graphically and displayed according to individual points of view. Therefore you can combineany variables even historical ones for trend analysis.

Click on the Navigation tab or on the plant name (XXX Plant) in the Navigation Tree to start the Plant Overview.

Click on single values or select all values of a column by clicking on the column header. Your selection is marked by color and you can add values by pressing the <Ctrl> key and clicking simultaneously.

Right click on the selection to display the context menu.

Color Description

green message

yellow warning

red alarm


40

Click History to display a diagram based on the selected values.

Click in the diagram to display the integrated ruler, which shows the numeric values of the curve(s).

Click and drag a frame in the diagram to zoom in or out.

Create and print a diagram for documentation at any time.

Click on the Tools button for the corresponding options. You can save and reload up to seven individually created diagram settings.

41Operation


5.4 Overview of Operating Sequences

The default operation in DASGIP Control includes the following steps:


42

43Quick Start


6 Quick Start

6.1 Short Instructions

Start DASGIP Control.

6.1.1 Calibration and Cleaning

Perform Calibrations and Cleaning with the bioprocess controller.

6.1.2 Start a New Workflow

Define a New Workflow for a process.

Click on File in the menu bar and choose New Workflow or click on the corresponding symbol.

There are two ways to start a workflow:

1. Select an already pre-defined template from the Default Template tab, which suites the workflow of the bioprocess controller best.

– The template names, which can vary from system to system, are listed right aside Selection. (Fordetailed information regarding the creation of templates refer to DASGIP Control User Manualchapter 3).

– Find a short description of the template aside Description.

Select the number of Reactors from the drop-down menu.

Please perform calibrations and cleanup using the bioprocess controller panel as usual. Due tolimited network protocols DASGIP Control cannot provide support for these procedures yet.

Quick StartDASware® migrateEnglish (EN)

44

Click the Open button to continue.

2. The User Template tab gives a list off all user defined workflows. Alternatively, you can select one of these workflows.

Select a workflow from the list.

You can adjust the workflow name aside Identifier.

Select the number of Reactors from the drop-down menu.– If the list consists of a lot of workflows, you can enter Search text and start searching with a click on

the Start Search button.– Additionally, you can select workflows from the Last modified drop down list. Click on New Search to

start the search with the new criteria.

Click the Open button to continue.

45Quick Start


DASGIP Control displays the Workflow Builder, which provides further options to adapt the workflow.

You can edit the names of each setup in the middle individually.

Click beneath Setup Identifier to enter the name.

You can change the mapping and the status of each setup by the drop down list of each edit box. The StateFree indicates available units and occupied indicates a process in progress. You can not start a new processfor an occupied unit.

At the right you can adjust more details.

DASGIP Control accepts only well-defined workflow Identifiers with limited special characters. Especially, ifyou want to use a workflow as template, we recommend using self-evident identifier.

DASGIP Control maintains the State automatically. Usually there is no need to change the statemanually.


46

Choose the appropriate vessel type from the drop down list next to Reactor or choose the universal vessel type UNI 1000.

Optionally, you can edit the displayed Comment.

6.1.2.1 Save as Recipe Template

You can use each created workflow as template for further processes.

Click on the checkbox next to Save as Recipe Template to use the current workflow as template.

Click the Save button to continue.

Click the Yes button in the following dialog to confirm the changes.

DASGIP Control displays each recipe template beneath Workflow > Template in the Navigation Tree and onthe User Template tab when you start a new workflow (Fig. 6.1.2) or run a workflow (Fig. 6.1.4).

47Quick Start


Choose a template and click the Run button.

6.1.3 Recipe Editor

DASGIP Control opens the Recipe Editor Fig. 6-1. You can complete and adjust all recipe settings e.g.pre-defined set-points for control loops and actors at this point.

Fig. 6-1: Recipe Editor

The Recipe Editor comprises several columns and one row for each setup (vessel). Corresponding to thepre-selected workflow, the profile cells are already allocated and values entered.


48

Click on single cells to display the corresponding parameters on the right side. Please refer to the User Manual DASGIP Control chapter 3.2.1 for more details.

Click the Save button to continue.

Click the Yes button to start the first procedure immediately.

6.1.4 Process Start

Choose Run Workflow from the File menu or click on the corresponding symbol to start each already set up workflow.

Already created and saved workflows are listed on the Workflows waiting to be started tab.

You can also adjust settings during process later on, but please note, that these changes will notbe stored for documentation. White recipe settings are part of the batch report in MicrosoftExcel®.

49Quick Start


The User Templates tab lists all created and saved workflow templates.

Select a workflow and click the Run button to continue.

Subsequently, you can change the Workflow name, the number of Units, and Setup names.

Click the Run button to continue.

The process starts and DASGIP Control displays all relevant functions in a Summary View.(5.3.2.1).

6.2 Documentation

DAGIP Control logs all data and events in one central database (SQL). Further features such as export intocsv files via one mouse click and strong charting capabilities ease plotting and data comparison in anunrivalled manner.


50

6.2.1 Data Export

You can export all process data from completed processes.

Click on the Overview tab and open the Finished branch of the Navigation Tree.

Open the context menu by right mouse click.

Click on Load to load data.

A right mouse click on single procedures opens the next context menu. Use Export (CSV) to export data as csv file.

DASGIP Documentation Wizard eases the editing of csv files in Microsoft Excel®.

6.2.2 DASGIP Documentation Wizard

DASGIP Documentation Wizard is a Microsoft Excel® Add-In and provides an easy and convenientevaluation of exported files including a graphical presentation. Automatically generated online cultivationreports of current and historical runs can be compared to each other. This allows the chart creator to createtrends populated with the selected tracks i.e. pH and dissolved oxygen (DO) from a subset of runsautomatically. Consider selecting duration or inoculation time to share a common x-axis.

DASGIP Documentation Wizard generates charts that can be formatted in the usual way usingthe Microsoft Excel® chart properties.

51Quick Start


DASGIP Documentation Wizard helps you to select the necessary columns in the experiment data tablesheet and accomplishes also the following features:

• The time axis has the suitable format and scale for the chosen range.

• The axis of the process values is labeled.

• The chart and the corresponding sheet in the working folder are labeled according to the chosen options.

• The markers for data points (graphs), which are usually shown in Microsoft Excel® x-y-charts, areremoved. Due to the amount of data generated by DASGIP Control they are optically distracting andslow down processing speed. If needed, they can be added.

6.2.2.1 Installation

Each DASGIP Control PC includes an already installed DASGIP Documentation Wizard. The installation onan additional computer requires Microsoft Excel® in the version XP or higher.

Please refer to the User Manual DASGIP Documentation Wizard for more details.

6.2.2.2 Create a Chart

The DASGIP Documentation Wizard window is subdivided in three parts:

• Time axis displays a list of available units for the x-axis at the left.

• Value axis displays a list of available process values and setups (Channels) for the 1. y-Axis in the middle.

• Additionally, chart Options can be selected at the right.

Define the time axis for the chart. Timestamp is the default selection. Duration displays the process time and Inoculation Time the time since inoculation.


52

Select the process values from the list of 1.Axis for the chart. Click on the value names for selection and press the <Ctrl> key while highlighting with the mouse to select additional entries (selection).

If you require an additional y-axis, click on the Options tab the check box next to 2. Y-Axis. You will get an additional column of process values for the 2. axis.

Select the setups from Channels for the chart. Press the <Shift> key while highlighting with the mouse to select the range of entries between the existing highlighted entry and the next selected entry (selecting a range).

Use the corresponding check boxes of the Options and 1. Axis tabs to select certain features or trend linesfor the chart.

Check the option 2. Y-Axis to display an additional 2. Axis tab on the right.

Click the New Chart button to create a new data sheet with the chart.

Highlight at least one entry in each of the shortlists to create a chart.

53Quick Start


6.2.2.3 Process Documentation

In addition to the charts, the Microsoft Excel® working folder contains tables with information regardingWorkflow, Procedures and the individual Setups, the recorded Data and Events. This information is shown ondifferent sheets.

The Procedure sheet contains all configuration data, which has been defined as starting values for this procedure during the workflow configuration:

• Controller settings

• Cascade settings

• Descriptions

• Alarm settings (optional)

• Data mining properties (optional)

The upper part of the table shows general information regarding the procedure. The lower one showsfeeding and control parameters from the profile headers of the Recipe Editor.


54

The sheets Setup 1, 2, ... contain the configuration data of the individual setups. This contains:

• Profile definitions

• Calibration values

• Trigger settings (optional)

The sheets Data1, Data2, … include all process values, which where recorded during the runtime of aprocedure

To reduce the data amount, values are just stored after significant changes or after a time span ofapproximately 5 – 60 minutes.

55Quick Start


6.2.2.4 Fill Empty Data Cells

The data log interval and the data reduction feature is the reason for empty data cells on the data sheet.Empty cells result in errors when the data columns are used for calculations in Microsoft Excel®. To avoidthis DASGIP Documentation Wizard can fill cells automatically.

Click on the Add-Ins tab and select Dasgip > Data Column Manager.

Click the radio button next to fill with last value to fill the empty cells with the previous value or the one next to interpolate liear to fill the cells with an interpolated value.

Click on the Fill Button to start.


56

The new values of the chosen Setup (1) are displayed in blue color on the data sheet Data (1).

You can undo the cell filling.

Open the Daten Column Manager again and click the Undo button pm the Fill tab.

6.2.2.5 Add Data Column, Low Pass and/or Derivation

You can enter additional data columns, low pass filter and/or derivations by the Data Column Manager.

Start the Data Column Manager as described before.

Click on the Add Column tab and add the Name, the Unit and the number of Digits for the column.

57Quick Start


Select the radio button next to Procedure to add a column regarding to a procedure, which will be displayed on the Data sheet.

Select the radio button next to Setup to add a column to each setup, which will be displayed on the Data1-x sheets at the end of the existing columns.

Click on the Low Pass tab and select a value from Value axis that will be displayed as Name.


58

Define the unit of the low pass (hh:mm:ss) in the edit box of T:

Switch between the tabs by clicking on the arrows in the upper right corner.

Define a derivation with respect to time on the d/dt tab.

Click on the d/dt tab and select a value from Value axis that will be displayed as Name.

Select a Unit from the drop down list per day, pre hour or per minute.

59Quick Start


Define the number of Digits.

6.2.2.6 Event Documentation

The Events data sheet shows a list of all events that were recorded during the runtime of a process.

Events are sorted according to the timestamp. The different colors of the rows reflect the type andimportance of the even s. 5.3.2.1.

After the data export in Microsoft Excel® you can print charts and/or the complete process documentation.


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61Troubleshooting


7 Troubleshooting

7.1 Error Search

7.1.1 Bioprocess controller is not accessible

Check list:

Check, if the process computer is connected to the network (Ethernet).

Check, if the controller is connected to the device server (i.e. Moxa) for New Brunswick and Applikon or directly to the process computer for Sartorius.

Check, if the device server (i.e. Moxa) is switched on.

TroubleshootingDASware® migrateEnglish (EN)

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IndexDASware® migrate

English (EN)63

Index

A

Abbreviations .........................................................4

AlarmSettings ...........................................................36

Alarm OptionDASGIP Control ..............................................36

B

Bioprocess ControllerNot Accessible ................................................61Remote Function .............................................23Settings ...........................................................25

C

Calibration .................................................... 43

Cleaning ...............................................................43

ColorsEvent Monitor .................................................38Symbols ..........................................................29

D

DangerLevels ................................................................3Symbols ............................................................3

DASGIP ControlAlarm Option ...................................................36Chart Control Panel .........................................37Event Monitor .................................................38First Steps .......................................................23Login ...............................................................23Operation ........................................................23Overview .........................................................24Process View ...................................................30Summary View ................................................27

DASGIP Documentation Wizard ...........................502. Y-Axis ..........................................................52Add Data Column ............................................56Add Derivation ................................................56Add Low Pass ..................................................56Create Chart ....................................................51Fill Empty Data Cells .......................................55

Installation ...................................................... 51

DASware access .................................................... 7

DASware analyze ................................................... 8

DASware connect .................................................. 9

DASware design .................................................. 10

DASware discover ............................................... 11

DASware migrate ................................................. 12Main Illustration ............................................. 14Product Description ....................................... 13Technical Data ................................................ 15

DASware SolutionsOverview ........................................................... 5

DefinitionParameter ....................................................... 33Set-point Profiles ............................................ 34

Delivery Package ................................................. 15

Documentation .................................................... 49Create a Chart ................................................ 51Data Export .................................................... 50Events ............................................................. 59

E

Edit boxes ............................................................ 30

Event Monitor ...................................................... 38Colors ............................................................. 39

ExportData ................................................................ 50

F

Features ............................................................... 15

Format Conventions .............................................. 4

G

Glossary ................................................................. 4

I

InstallationDASGIP Documentation Wizard ..................... 51DASware migrate ........................................... 19

IndexDASware® migrateEnglish (EN)

64

Intended Use ........................................................17

Interfaces ..............................................................19Applikon ..........................................................20New Brunswick ...............................................19Sartorius ..........................................................20

IP Address ............................................................26

L

LoginDASGIP Control ................................................3

M

Main Illustration ...................................................14

Material Damages ..................................................3

N

Navigation Area ....................................................24

O

Offline Values .......................................................37

Operating Sequences ...........................................41

OverviewDASGIP Control ..............................................24DASware Solutions ...........................................6

P

ParameterDefinitions .......................................................33Sensor .............................................................33Vessel ..............................................................33

Plant Overview .....................................................39History ............................................................40

ProcessStart ................................................................48

Process Documentation .......................................53

Process View ........................................................30

Product DescriptionDASware migrate ............................................13

Profile

Constant Value ............................................... 35Data Table ................................................ 35Function ......................................................... 35

Profile Source ...................................................... 35

Program Start ...................................................... 23DASGIP Control ................................................ 3

Q

Quick Start ........................................................... 43

R

Recipe Editor ....................................................... 47

S

Safety ................................................................... 17

Save as Recipe Template ..................................... 46

Save User Chart Settings................................. 38

Set-point ProfileDefinition ........................................................ 34

SettingsAlarm .............................................................. 36Bioprocess Controller ..................................... 25

Setup Identifier .................................................... 45

StartNew Workflow ................................................ 43Process ........................................................... 48Workflow ........................................................ 44

Summary View .................................................... 27

SymbolColors ............................................................. 29Controller ....................................................... 34Vessel ............................................................. 33

T

TabData ................................................................ 35Default Template ............................................ 43Graph .............................................................. 35Navigation ...................................................... 39Type ................................................................ 35User Template ................................................ 44

IndexDASware® migrate

English (EN)65

Troubleshooting ...................................................61

U

User Instructions ............................................. 3

V

VesselParameters ............................................... 33

W

WorkflowNew......................................................... 43

Workflow Builder .................................................45

Documents

Buch DASware migrate en - User Manual Search …...DASware migrate integrates most common 3rd party bioreactor control units and systems (e.g. Sartorius Stedim Biotech & Applikon)