75
BSC1010C Final Lab Practical Review Click on enter to see the answer. Click enter again to move to next slide.

BSC1010C Final Lab Practical Review

  • Upload
    lorin

  • View
    43

  • Download
    0

Embed Size (px)

DESCRIPTION

Click on enter to see the answer. C lick enter again to move to next slide. BSC1010C Final Lab Practical Review. What was the substrate in the enzyme experiment?. Hydrogen Peroxide (H 2 O 2 ). What was the enzyme in the enzyme experiment?. catalase. - PowerPoint PPT Presentation

Citation preview

Page 1: BSC1010C Final Lab Practical Review

BSC1010C Final Lab Practical Review

Click on enter to see the answer.Click enter again to move to next slide.

Page 2: BSC1010C Final Lab Practical Review

What was the substratein the enzyme experiment?

Hydrogen Peroxide (H2O2)

Page 3: BSC1010C Final Lab Practical Review

What was the enzyme in the enzyme experiment?

catalase

Page 4: BSC1010C Final Lab Practical Review

What was the source of thisenzyme in the enzyme lab?

Sheep’s blood

Page 5: BSC1010C Final Lab Practical Review

What was/were the product(s)in the enzyme experiment?

Water and oxygen gas

Page 6: BSC1010C Final Lab Practical Review

What was the optimum pHfor the enzyme?

pH 7

Page 7: BSC1010C Final Lab Practical Review

Why is the enzyme less active at very high or very low pH?

Enzyme is denatured and reaction stops

Page 8: BSC1010C Final Lab Practical Review

What was the optimum temperaturefor the enzyme experiment?

40 C

Page 9: BSC1010C Final Lab Practical Review

Why do you see little gas produced at low temperatures?

Enzyme and substrate molecules are moving very slowly, so less enzyme-substrate collisions,

So less reactions

Page 10: BSC1010C Final Lab Practical Review

Why do you see little or no gas production at high temperatures?

The enzyme, which is a protein, is denaturedat high temperatures and reaction stops.

Page 11: BSC1010C Final Lab Practical Review

What did we measure to determinethe rate of the enzyme reaction?

Oxygen gas pressure – rate of oxygen gas production

Page 12: BSC1010C Final Lab Practical Review

What was the control in the enzyme experiment?

Distilled water in place of enzyme

Page 13: BSC1010C Final Lab Practical Review

What was the inhibitor used in enzyme experiment?

Copper sulfate (CuSO4)

Page 14: BSC1010C Final Lab Practical Review

What should happen when the inhibitor is added in the enzyme experiment?

Reaction should stop as the inhibitor is preventing the substrate and enzyme from coming together

Page 15: BSC1010C Final Lab Practical Review

What happened to the enzyme reaction whenthe temperature was raised to 60 C?

Reaction stopped because enzymewas denatured

Page 16: BSC1010C Final Lab Practical Review

What is this plant?

Coleus

Page 17: BSC1010C Final Lab Practical Review

What is the name of the pigmentin these leaves that is water-soluble?

What color is this pigment?

Anthocyaninred

Page 18: BSC1010C Final Lab Practical Review

What pigment is alcohol-soluble?What color is this pigment?

Chlorophyllgreen

Page 19: BSC1010C Final Lab Practical Review

What was used to indirectly detectphotosynthesis in these leaves?

IKI

Page 20: BSC1010C Final Lab Practical Review

What areas of the leaf carried out photosynthesis according to IKI test?

Green areas (where chlorophyll was located)

Page 21: BSC1010C Final Lab Practical Review

What is the name of the techniquethat uses this setup?

chromatography

Page 22: BSC1010C Final Lab Practical Review

What was this setup used toseparate?

Plant pigments

Page 23: BSC1010C Final Lab Practical Review

What is the genus of plant used to get these pigments from the leaves?

Magnolia

Page 24: BSC1010C Final Lab Practical Review

Name 3 things that determine the movement of the pigments up the paper.

1. Size of pigment molecule (molecular weight)2. Solubility of pigment in the solvent

3. H bonding of pigment to paper

Page 25: BSC1010C Final Lab Practical Review

What causes the solvent tomove up the paper?

Capillary action (adhesion + cohesion)

Page 26: BSC1010C Final Lab Practical Review

What is used to set 100%in absorption spectrum experiment?

acetone

Page 27: BSC1010C Final Lab Practical Review

What is the equation forcell respiration? (balanced, please)

C6H12O6 + 6 O2 6 CO2 + 6 H2O

Page 28: BSC1010C Final Lab Practical Review

What is the balanced equationfor photosynthesis?

6 CO2 + 6 H2O C6H12O6 + 6 O2

Page 29: BSC1010C Final Lab Practical Review

Given cell respiration and photosynthesis,which happens at all times?

Cell respiration

Page 30: BSC1010C Final Lab Practical Review

Given cell respiration and photosynthesis, which happens only in the light?

photosynthesis

Page 31: BSC1010C Final Lab Practical Review

Where is photosynthesis occurringin the following graph?

A and C

(Photosynthesis is taking in CO2 so level falls)

A B C D

Page 32: BSC1010C Final Lab Practical Review

What happens to CO2 levels in the respirometerin the light? WHY?

Decline Because photosynthesis is taking in CO2.

Page 33: BSC1010C Final Lab Practical Review

What happens to CO2 levels in the respirometerin the dark? WHY?

They go up.Photosynthesis is NOT occurring so only cell

respiration which is producing CO2

Page 34: BSC1010C Final Lab Practical Review

What genus of plant was used in the cell respiration – photosynthesis experiment?

Viburnum

Page 35: BSC1010C Final Lab Practical Review

What is the purpose of the beaker of water that was placed between the light source and the leaves?

To act as a heat sink (absorb the heat given off by the light)

Page 36: BSC1010C Final Lab Practical Review

What are the 2 typesof nuclear division?

Mitosis and Meiosis

Page 37: BSC1010C Final Lab Practical Review

In mitosis, how many cells are formed?What is their chromosome #?

2 cells

Diploid number

Page 38: BSC1010C Final Lab Practical Review

In meiosis, how many cells are formed?What is their chromosome #?

4 cells

Haploid #

Page 39: BSC1010C Final Lab Practical Review

What is the name of this region?

Root cap

Page 40: BSC1010C Final Lab Practical Review

What is the name of this region?

Region of maturation or differentiation

Page 41: BSC1010C Final Lab Practical Review

What is the name of this region?

Region ofelongation

Page 42: BSC1010C Final Lab Practical Review

What is the name of this region?

Apical meristemOr

Region of cell division

Page 43: BSC1010C Final Lab Practical Review

What is this stage? Is it a plant or animal cell?

Anaphase

Plant cell

Page 44: BSC1010C Final Lab Practical Review

What stage is this?What is arrow pointing to?

Telophase

Cleavage furrow

Page 45: BSC1010C Final Lab Practical Review

What is this phase?Plant or animal cell?

Prophase

Plant cell

Page 46: BSC1010C Final Lab Practical Review

What is this phase?Plant or animal cell?

Metaphase

Plant cell

Page 47: BSC1010C Final Lab Practical Review

What is this phase?What is arrow pointing to?

Telophase

Cell plate of plant cell

Page 48: BSC1010C Final Lab Practical Review

What is this phase?Plant or animal cell?

Anaphase

Animal cell

Page 49: BSC1010C Final Lab Practical Review

What is this phase of meiosis?

Metaphase I

Page 50: BSC1010C Final Lab Practical Review

What is this phase of meiosis?

Anaphase I

Page 51: BSC1010C Final Lab Practical Review

What is this phase of meiosis?

Telophase I

Page 52: BSC1010C Final Lab Practical Review

What specimen was used to study mitosisin animals?

Whitefish blastula

Page 53: BSC1010C Final Lab Practical Review

What specimen was used to studymitosis in plants?

Onion root tip

Page 54: BSC1010C Final Lab Practical Review

What cell division process occursin somatic cells?

mitosis

Page 55: BSC1010C Final Lab Practical Review

What cell division process producesgametes and spores?

meiosis

Page 56: BSC1010C Final Lab Practical Review

What cell division process is precededby S in the cell cycle?

Both Mitosis and Meiosis I

Page 57: BSC1010C Final Lab Practical Review

How many chromosomes are in the cell below?

2

Page 58: BSC1010C Final Lab Practical Review

What is this phase of meiosis?

Prophase I

Page 59: BSC1010C Final Lab Practical Review

What are the monomers that make up DNA?

nucleotides

Page 60: BSC1010C Final Lab Practical Review

If a piece of DNA has 4 sites recognized by the enzyme, how many fragments will be produced?

5

Page 61: BSC1010C Final Lab Practical Review

Enzymes that cut up DNA are called ___.

Restriction enzymesOr

Restriction endonucleases

Page 62: BSC1010C Final Lab Practical Review

Name an example of these enzymes.

EcoRI

HindIII

Page 63: BSC1010C Final Lab Practical Review

What produces the enzymes that cut up DNA?Why?

Bacteria

To cut up invading phage (bacterial viruses)

Page 64: BSC1010C Final Lab Practical Review

What is the source of the DNA used in this experiment?

Bacteriophage lambda

Page 65: BSC1010C Final Lab Practical Review

What is this piece of equipment?

micropipet

Page 66: BSC1010C Final Lab Practical Review

What is the volume this is set to measure(specify units)?

50 L

Page 67: BSC1010C Final Lab Practical Review

What is the purpose of the 2 “stop points”?

1st Stop – take up the liquid from tube2nd Step – dispense the liquid into new tube

Side blue button = ejects the tip

Page 68: BSC1010C Final Lab Practical Review

What technique is used to separatethe cut up pieces (fragments) of DNA?

electrophoresis

Page 69: BSC1010C Final Lab Practical Review

What is the name of the gel thatis used to support the fragments during

this separation?

agarose

Page 70: BSC1010C Final Lab Practical Review

Convert 0.6 mL to L

600 L

Page 71: BSC1010C Final Lab Practical Review

What is the size (in bp) of this fragment?

6,527 bp

Page 72: BSC1010C Final Lab Practical Review

Given the following fragments:500 bp 5,000 bp

2500 bp 25,000 bp Which fragment in the gel below is

the 25,000 bp fragment?

A

A B C D

Page 73: BSC1010C Final Lab Practical Review

What is the charge on DNA?

What gives it this charge?

Negative

The phosphate group

Page 74: BSC1010C Final Lab Practical Review

What are the places where the enzyme cuts DNA called?

Restriction sites

Page 75: BSC1010C Final Lab Practical Review

A piece of DNA was cut by 3 different enzymes. The standard is in well A. How many “cuts” were made by the enzyme in well B?

Two cuts

A B C D