University of the Philippines VisayasCollege of Arts and Sciences

Division of Biological SciencesPublic Health Program

PH 177Medical Entomology

Laboratory Exercise No. ____

Names: Date Conducted:________________

Mosquito Species in Brgy ______________________

This is a field investigation to mainly survey presence and identify mosquito species in the assigned community, specifically to:

1. determine mosquito abundance and Rate of Capture of the adult specimen,2. establish the following mosquito larva and pupa indices:

a. House Indexb. Breteaux Indexc. Container Index,

3. collect sample specimen and classify these to the genus level based on physical markers only, and

4. prepare a slide of an egg, pupa larva and adult specimen for confirmation

You need magnifiers to examine and count the specimen during the survey, containers with 10% formalin for your larva collection, slides and cover slips for your slide preparation, Canada Balsam for permanent slides, water for temporary slides(when identifying the genus of the larva) and droppers to transfer larvae or pupae from container to slides. For adult specimen, white clothing could facilitate detection and counting of fallen adult specimen. Reminder: Chemical sprays could best help collect the adults stages. Catching with your bare hands should be avoided for your safety, that would keep you also from damaging the specimen.

You could use the simplified form of the indices that follows, yet a more comprehensive formula could be obtained elsewhere as an option (just include your sources in the Reference section of the report).For rural areas with scattered houses at least 15 houses distributed evenly over the whole area of your baranggay could be used for the indices.

A. Larval/pupal investigation:

a. House index- percentage of houses with mosquito larvae/pupae found inside the house

No.of Houses InfestedHI = ----------------------------------------- x 100

No. of House

b. Breteaux Index- percentage of containers positive for mosquito larvae or pupae per 100 houses inspected ( in percentage)

BI = Number of container positive for larva or pupae 100 houses inspected

c. Container index – percentage of water holding containers positive for larvae of pupae

No. of Positive containersCI= ---------------------------------------- x 100

No. of containers inspected

B. Adult investigation:a. Mosquito abundance based on landing rate

i. Indoorii. Outdoor

b. Rate of Capture per houri. Indoorii. Outdoor

C. Species Identification Possible species of the specimen:________________________Proof: (if Larvae) ______________________ Proof: ( ifPupa) ______________________Proof: (if Adult)_________________________

* for more than 1 genera just recopy the C section per species identified and provide the pertinent evidence (slides)

D. Submit slides of mosquitoes specimen in all forms.(egg, larva, pupa, adult)

References:

Enjoy Community Work!

Prepared and revised by:Mary Ann F. Naragdao, MSc in Commnity Health and Epidemiology//Mafn 2013