Brainstorming…

Concerns

Environmental or medicinal

Applicable/relevant

Novel????Elegant????

Recombinant/biosynthetic work

Possibilities

Plastic/cellulose conversion to biodiesel via microbes and/or their products

Enzymatic degradation of biofilm via toothpaste reagents

Quick indication of successfultransformation (such as floatingbacteria)

Others…

Targeting termites via microbiot-icides

Temp-sensitive chromo/fluorescent microbe (thermometer)

Chromosome counter

Fluorophores and quenchers…

Intriguing

Melanoma/SCC/BCC detection…-currently, visual and biopsy

Hypothetical-visual, chromotest, biopsy

But how…?

Initial thoughts:-differential

-live vs. dead (trypan blue stain)

-mitotic index (DNA Hoechst stain)

-keratin differentiation (protein

Ponceau S stain)

How to build in an assay???

HPV Detection

Irregular growth & acetic acid test

Overexpress putatitive HPV receptor (HSPG) on S. epidermidis

Link it intracellular amplification regimen

CRE recombinase is expressedExcises floxed E. chromi colorColor is lost as floxed color is

removed from promoter

Cell Aging

Telomere length = division surrogate

Circadian rhythm events???

Protein profiles???