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Preventative and Therapeutic Treatment of Resveratrol on Alzheimer’s Disease using CL2006 and CL4176 C. elegan models. Bradford Greaves. Need. http://www.searo.who.int/en/Section1174/Section1199/Section1567/Section1823_8066.htm. The APP Processing. - PowerPoint PPT Presentation
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Preventative and Therapeutic Treatment of Resveratrol on
Alzheimer’s Disease using CL2006 and CL4176 C. elegan models
Bradford Greaves
Need
http://www.searo.who.int/en/Section1174/Section1199/Section1567/Section1823_8066.htm
The APP Processing
Senile Plaques
Modified from Laurén et al., Nature (2009)
Figure 1
Resveratrol Promotes Clearance of Alzheimer’s Disease Amyloid- Peptides, Marambaud 2005
The β amyloid peptide can act as a modular aggregation domain, Link 2008
Alzheimer’s Disease cont.
http://www.nia.nih.gov/NR/rdonlyres/A294D332-71A2-4866-BDD7-A0DF216DAAA4/0/Alzheimers_Disease_Unraveling_the_Mystery.pdf
Oxidative Damage
http://www.nia.nih.gov/NR/rdonlyres/A294D332-71A2-4866-BDD7-A0DF216DAAA4/0/Alzheimers_Disease_Unraveling_the_Mystery.pdf
Purpose
To preventatively and therapeutically treat Alzheimer’s Disease with resveratrol using CL2006 and CL4176 C. elegan models
Hypothesis
Alternate Hypothesis: Both preventive and therapeutic treatment of resveratrol on CL2006 will lead to a decrease in amyloid beta production and for CL4176 there will be a delay of the onset of paralysis.
Null Hypothesis: There will be no change in amyloid beta production nor paralysis with the addition of resveratrol.
Resveratrol’s Coloration With Alzheimer’s Disease
http://www.chiro.org/nutrition/ABSTRACTS/Resveratrol_in_Prevention_and_Treatment.pdf
Figure 2
Soy isoflavone glycitein protects against beta amyloid-induced toxicity and oxidative stress in transgenic Caenorhabditis elegans, Gutierrez-Zepeda 2005
Transgenic C. elegans as a Model in Alzheimer’s Research, Wu 2005
Vendor Cat # Product Quantity
Unit $ Total $
Sigma-Aldrich Online Catalog
R5010-500MG
Resveratrol 1 $281.50 $281.50
Sigma-Aldrich Online Catalog
S2188-1MG γ-Secretase inhibitor 1 $267.50 $267.50
Sigma-Aldrich Online Catalog
P6148 Paraformaldehyde 1 $28.00 $28.00
Sigma-Aldrich Online Catalog
P5368 PBS 1 $42.60 $42.60
Sigma-Aldrich Online Catalog
M6250 2-Mercaptoethanol 1 $23.00 $23.00
Sigma-Aldrich Online Catalog
T9284 Triton® X-100 1 $48.70 $48.70
Sigma-Aldrich Online Catalog
T0574 Trizma® Buffer solution
1 $25.90 $25.90
Sigma-Aldrich Online Catalog
TI892-25G Thiflavin S 1 $27.80 $27.80
Carolina 2009 173525 C. elegan culture kit 1 $73.00 $73.00
Carolina 2009 173500 N2 control 3 $10.95 $32.85
University of Minnesota CL4176 C. elegan paralysis 20
University of Minnesota CL2006 C. elegan staining assay 20
Do Ability
• CL2006 and CL4176: Staining and Paralysis• Control food and Resveratrol available• Fluorescent Microscope at school
• Prism not known (GFP)
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