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DUSHYANT KUMAR
BSA-08-613
Plant antibiotic production
through plant tissue culture
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Antibiotics
These are the chemical compounds which arehaving the property
act against the biologicaldisease producing agents likely bacteria,
virus andfungus.Example of antibiotics
Antibiotic plant spp.
Quinine cinchona
Barberin Papaver somniferum
Artemisin Artimesia spp.
Ajmalicin Cartharanthus roseus
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teps o pro uct on
Production strategy consist of two distinct phase
Growth phase for cell biomass accumulation
Production phase for biosynthesis and accumulation
of biochemical (antibiotic)
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Nutrient medium
Linsmaier and skoog medium (LS medium).
It contain Basal salt mixture containing micro andmacro elements
with vitamins .
All high value biochemicals from cultured plantcells are
secondary metabolities.
Biochemicals production by plant cells is markedly
influenced by the constituent of culture medium
&temp.,light,inoculum size.
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Improving biochemicals production
Suitable culture medium & conditions.
Development of high producing cultures.
Use of elicitors.
Use of organ cultures.
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Biochemical content of cell cultures depends onthe plant species
the genotype or strain of thespecies .
Cell cultures are highly heterogeneous forbiochemical production
in that different cellsshows different levels of production .
This variation is used to advantage by screening alarge nu. Of
clones for isolation of high producingclones
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Often the high yielding clones so isolated exhibit adecline in
production levels on being maintained byserial subculture but in
several cases stable high
producing clones have been successfully isolated.For production
of biochemicals from plant cell
cultures since it greatly reduces the cost ofproduction.
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PREPARATION OF PLANT TISSUE
CULTURE MEDIUM
Measure approximately 90% of the requiredvolume of the
deionized-distilled water in aflask/container of double the size of
the required
volume.Add the dehydrated medium into the water and
stir to dissolve the medium completely. Gentleheating of the
solution may be required to bring
powder into solution
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Add desired heat stable supplements to the mediumsolution.
Add additional deionized-distilled water to the
medium solution to obtain the final required volume.Set the
desired pH with NaOH or HCl.
Dispense the medium into culture vessels
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Sterilize the medium by autoclaving at 15 psi (121C)for
appropriate time period.Higher temperaturemay result in poor cell
growth.
Add heat labile supplements after autoclaving.
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