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 Visit us on the Web at discover.bio-rad.com

Call toll free at 1-800-4BIORAD (1-800-424-6723)

Doing ELISA’s?Cost/kit # Samples # of Targets Total # Cost per Sample Dynamic

of Assays sample/target Volume Range*

ELISA $500 80 1 80 $6.25  >1 ml 2 – 3,000pg/ml

$600 80 1 80 $7.50  >1 ml 

Bio-Plex $475 80 1 80 $5.94 12.5 – 50µl 2– 32,000pg/ml

4-Plex $920 80 4 320 $2.88 12.5 – 50µl  2–32,000pg/ml 

8-Plex $1,490 80 8 640 $2.33 12.5 – 50µl  2–32,000pg/ml 

17-Plex $2,485 80 17 1360 $1.82 12.5 – 50µl  2–32,000pg/ml 

27-Plex $3,670 80 27 2160 $1.70  12.5 – 50µl  2–32,000pg/ml

*Approximate range of standard curves. Experimental range dependent on assay target and sample matrix.

 

 Ask me for a quote today. 

Christina Anderson

christina_anderson@bio-rad.com

1-800-876-3425 x8514

Cost Comparison Bio-Plex Phosphoprotein Assays vs. Western BlotsBio-Plex Assay Costs 

Single Target Three Targets Five Targets

  Assay $285.00 $855.00 $1,425.00

Reagent kit $235.00 $235.00 $235.00

Lysis kit $163.00 $163.00 $163.00

  Total cost per assay plate $683.00 $1,253 $1,823.00 Estimated labor costs

Cost per sample (96-well assay) $7.11 $12.05 $18.98 Labor rate/hr $25.00

Cost per data point (96-well assay) $7.11 $4.35 $3.80 Hours hands on time 1.50  Total cost per data point pus labor $44.61 $41.85 $41.30 Total labor costs $37.50

One Target Three Targets Five TargetsWestern Blot Costs (midi-gel) Single Blot Three Blots Five Blots

Pre-cast gel (12 wells to run) $14.70 $44.10 $73.50

Protein standards $2.10 $6.30 $10.50

  Transfer membrane and filter paper $9.50 $28.50 $47.50

Phosphoprotein primary antibody $40.95 $122.85 $204.75

Detection reagents $12.60 $37.80 $63.00

Buffers $3.15 $9.45 $15.75 Estimated labor costs

  Total cost per blot $83.00 $249.00 $415.00 Labor rate/hr $25.00

Cost per data point (1 sample per data point) $7.55 $22.64 $37.73 Hours hands on time 3.00

  Total cost per data point plus labor) $82.55 $246.64 $412.73 Total labor costs $75.00

Save Money, Time, and Sample with the Bio-Plex® Suspension Array System

8/14/2019 Bioplex ELISA Western Assays 2pg

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Rat

IL-1a •

IL-1b •

IL-2 •

IL-4 •

IL-6 •

IL-10 •

GM-CSF •

IFN-g  •

 TNF-a •

Bio-Plex®

Rat Cytokine, Chemo

and Growth Factor Assay

Bio-Plex Phosphoprotein and Total Target Assays Available Assays

CST antibodies exclusively developed

and validated for Bio-Plex phosphoprotein

and total target assays.

 

 Assays Phosphoprotein Total

 

 Akt (Ser473 ) ●  ●

  GSK-3α / β (Ser21 /Ser9 ) ● 

p53 (Ser15 ) ●  ● 

p53 (Ser37 ) ●  ● 

p53 (Ser46 ) ●  ● 

Histone H3 (Ser10 ) ● 

IκB-α (Ser32 /Ser36 ) ●  ●

NF-κB p65 (Ser536 ) ● 

STAT2 (Tyr689 ) ● 

STAT3 (Ser727 ) ● 

STAT3 (Tyr705 ) ● 

STAT6 (Tyr641 ) ●

Tyk2 (Tyr1054 /Tyr1055 ) ● 

IGF-IR (Tyr1131 ) ● 

IR-β (Tyr1146 ) ● 

IRS-1 (Ser636 /Ser639 ) ●

Akt Signaling

Cell Cycle/Checkpoint Control

Chromatin Regulation/Acetylation

Immunology/Inflammation

Glucose/Energy Metabolism

  Assays Phosphoprotein Total

 

 ATF-2 (Thr71 ) ●  ●

c-Jun (Ser63 ) ●  ●

ERK1 (Thr202 /Tyr204 ) ● 

ERK2 (Thr185 /Tyr187 ) ●  ●

ERK1/2 (Thr202 /Tyr204, Thr185 /Tyr187 ) ●  ●

HSP27 (Ser78 ) ● ●

JNK (Thr183 /Tyr185 ) ●  ●

MEK1 (Ser217 /Ser221 ) ●  ●

p38 MAPK (Thr180 /Tyr182 ) ●  ●

p90RSK (Thr359 /Ser363 ) ●  ●

 CREB (Ser133 ) ●  ●

TrkA (Tyr490 ) ● 

p70 S6 kinase (Thr421 /Ser424 ) ● 

S6 ribosomal protein (Ser235 /Ser236 ) ● 

Bcr-Abl (Tyr245 ) ●  ● 

c-Abl (Tyr245 ) ● 

c-Abl (Tyr412 ) ● 

EGFR (Tyr) ● 

PDGF receptor-β (Tyr751 ) ● 

Src (Tyr416 ) ● 

MAP Kinase Signaling

Neuroscience

Translational Control

Tyrosine Kinases

 

 Assays Human Mouse

IL-1α  ● 

IL-1β  ●  ● 

IL-1ra ● 

IL-2 ●  ● 

IL-2Rα  

IL-3 ●

IL-4 ●  ● 

IL-5 ●  ●

IL-6 ●  ● 

IL-7 ● 

IL-8 ● 

IL-9 ●  ● 

IL-10 ●  ● 

IL-12 (p40) ●

IL-12 (p70) ●  ●

IL-13 ●  ● 

IL-15 ● 

IL-16

IL-17 ●  ●

IL-18**

Basic FGF ● CTACK

Eotaxin ●  ● 

G-CSF ●  ●

GM-CSF ●  ● 

GRO-α 

 

 

 Assays Human Mouse

HGF

ICAM-1

IFN-α2

IFN- γ  ●  ● 

IP-10 ● 

KC ●

LIF

MCP-1 (MCAF) ●  ● 

MCP-3

M-CSF

MIF

MIG

MIP-1α  ●  ●

MIP-1β  ●  ● 

MIP-2

β-NGF

PDGF-BB  ● 

RANTES ●  ●

SCF

SCGF-β 

SDF-1α   TNF-α  ●  ● 

 TNF-β 

 TRAIL

 VCAM-1

 VEGF  ● 

 

 

Representative Working Assay Range

  Analyte Serum Plasma

IL-1b 0.2–516 0.2–566

IL-2 2.7–4,599 4.5–4,570

IL-4 0.3–2,611 0.2–2,611

IL-5 3.7–1,354 5.0–4,355

IL-6 1.7–16,058 5.2–18,618

IL-10 0.7–8,591 1.1–11,850

IL-12 (p70) 0.6–3,993 1.7–3,994

IL-13 0.2–1,745 0.3–2,406

IFN-g  0.5–1,814 0.7–1,814

 TNF-a 0.2–1,423 0.2–2,059

Bio-Plex Precision Pro™Human Cytokine Assay Panel

Bio-Plex Pro™ MagneticCytokine, Chemokine, and Growth Factor Assays

 Available Assays Assay Range and Sensitivity

Targets Range Sensit

lgG1 3.00–7,950 ng/ml 2 ng/m

lgG2 20.00–6,000 ng/ml 20 ng/

lgG3 5.25–5,000 ng/ml 0.8 ng/

lgG4 0.06–810 ng/ml 0.08 ng

lgA 0.12–500 ng/ml 0.2 ng/

lgE 0.01–100 ng/ml 0.02 ng

lgM 0.42–1,500 ng/ml 0.2 ng/

Specifications for ImmunoglobuIsotyping Assays

 The Bio-Plex suspension array system includes fuorescently labeled microspheres and instrumentation licensed to Bio-Rad Laboratories, Inc. by the Luminex Corporation.

 A Multitude of Choices

Human Human Human

  Acute Phase Angiogenesis Diabetes

a-2-macroglobulin Angiopoeitin-2 Adiponectin

CRP Follistatin Adipsin

Ferritin G-CSF C-peptide

Fibrinogen HGF Ghrelin

Haptoglobi n IL-8 GIP

Procalciton in Leptin GLP-1

SAA PDGF-BB Glucagon

SAP PECAM-1 IL-6

  Tissue plasminogen VEGF Insulin

activator Leptin

PAI-1

Resistin

TNF-a

Visatin

Bio-Plex Pro DiseaseResearch Assays

 Available Targets