GENE-UP® Assay Quick Guide | CRO Unit Dose

BIOMÉRIEUX

1

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Set Up Plate on the GENE-UP® Routine Software

Enrich Sample

Vortex for 5 minutesat 2,200 RPM(or with Scientific Industries Vortex-Genie® Pulse for 5 min at maximum speed.)

20 µL

Secure Lysis Tubes to Lysis Tube Holder.

Remove fromHeavy Rack Holder.

Place Lysis Tube Holder on Troemner vortex-type mixer with Troemner Vortex Mixer Adaptor (or on Scientific Industries Vortex-Genie® Pulse with the GENE-UP® Lysis Rack Adaptor)

Transfer enriched samples to Lysis Tubes.Place on Heavy Rack Holder.

Continue on back

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Lyse Samples

b)a)

c)

a) Home Screen b) Plate Map

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B I O M E R I E U X 4 1 4 5 7 1

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B I O M E R I E U X 4 1 4 5 7 1

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B I O M E R I E U X 4 1 4 5 7 1

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Assay / Media Sample Weight / Media Vol. Pre-warm

(ºC)Time (hrs)

Temp.(ºC)

Cronobacter / BPW

Infant formulas (liquids and powders), up to 25 g or 25 mL:- without probiotics: X g / 9X mL- with probiotics: X g / 9X mL + novobiocin (10 mg/L)

15-25 18-26

+37 ± 1Powdered infant formulas (up to 375 g): X g / 5X mL + novobiocin (10 mg/L) +37 ± 1 20-28

Environmental samples: X g / 9X mL 15-25 16-24

GENE-UP® Assay Quick Guide | CRO Unit Dose

0491

83-0

1 - e

n

2017

/11

376 Chemin de l'Orme69280 Marcy I´Etoile, France

673 620 399 RCS LYON Tel. 33 (0)4 78 87 20 00Fax 33 (0)4 78 87 20 90www.biomerieux.com

bioMérieux SA

Detailed information about these procedures are included in the GENE-UP® assay package inserts.

7

Run Plate

Spin PCR Tube Holder

View Results

42043 Buffered Peptone Water 6 x 225 mL42042 Buffered Peptone Water 6 x 90 mL42729 Minibags Buffered Peptone Water 6 x 225 mLAEB910303/4 Buffered Peptone Water 4 x 3 LAEB910305/2 Buffered Peptone Water 2 x 5 LAEB184150/10 Novobiocin 192 testsAEB520010 ESIATM 20 plates421580 CCI 20 plates421569 CSB 100 tubes20100 API® 20E 25 tests20160 API® ID32E 100 tests414057 GENE-UP® Lysis Kit 192 tests421920 GENE-UP® Cronobacter Kit 192 tests419194 GENE-UP® Tips (Biotix) 10 x 96 tips415180 Smasher XL Bags 2 L 400 bags415181 Smasher XL Bags 4 L 200 bagsAES400FB/50G Blender Bags 1500 bagsAES400P/50G Bags with Side Filter 1500 bagsDCPBF225B Closing Clips for Bags 50 clips

6

10 µL

Transfer Lysed Samples into PCR Tubes

a)

c)

Add lysed samples toPCR reagents.

Place and seal the optical strip caps onto each strip tube using Lysis Tube Remover Tool.

Biotix Tip

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< 1 Hour

10 sec.

4

5

b)

Remove the domed caps from the strip tubes.