Methods in Enzymology

Volume 133

Bioluminescence andChemiluminescence

Part B

William D . McElroyMarlene A . DeLuca

CONTRIBUTORS TO VOLUME 133 ix

PREFACE Xii i

VOLUMES IN SERIES X V

Section I . Bioluminescenc e

1 . Cloning Firefly Luciferase

JEFFREY R . DE WET ,KEITH V . WOOD ,

DONALD R . HELINSKI, AN DMARLENE DELUCA

3

2 . Extraction of Adenosine Triphosphate From Mi- PHILIP E . STANLEY

1 4

crobial and Somatic Cell s

3. Detection of Bacteriurea by Bioluminescence

BRUCE A . HANNA

2 2

4. Estimation of Biomass in Growing Cell Lines by ARNE LUNDIN ,Adenosine Triphosphate Assay

MICHAEL HASENSON ,J6RGEN PERSSON, AN DAKE POUSETTE

2 7

5 . Sensitive Bioluminescent Assay for a-Rungaro- JAMES M . SCHAEFFER

4 3

toxin Binding Site s

6 . Commercially Available Firefly Luciferase Re- FRANKLIN R . LEACH AN Dagents

JOANN J . WEBSTER

5 1

7. Cloning and Expression of the Genes from the CAROL MIYAMOTO ,Bioluminescent System of Marine Bacteria

MICHAEL BOYLAN ,ANGUS GRAHAM, AN DEDWARD MEIGHEN

7 0

8 . Techniques for Cloning and Analyzing Blolumi- JOANNE ENGEBRECHT AN Dnescence Genes from Marine Bacteria

MICHAEL SILVERMAN

8 3

9. Purification of Bacterial Luciferase by Affinity THOMAS O . BALDWIN ,Methods

THOMAS F . HOLZMAN ,RI IA B . HOLZMAN, AN DVICKI A . RIDDLE

9 8

10. Purification of Bacterial Luciferase by High- DENNIS J . O'KANE ,Performance Liquid Chromatography

MUSHTAQ AHMAD ,IAIN B . C . MATHESON, AN DJOHN LEE

109

11 . Bacterial Luciferase 4a-Hydroperoxyflavin Inter- SIIIAO-CHUN Tu

12 8mediates : Stabilization, Isolation, and Prop-ertie s

12 . Bacterial Luciferase Intermediates : The Neutral M . KURFÜRST, S . GHISLA ,Flavin Semiquinone, Its Reaction with Super- AND J . W . HASTINGS

14 0oxide, and the Flavin 4a-Hydroxide

13 . Purification and Properties of Lumazine Proteins Dr_NNIS J . O'KANE AN Dfrom Photobacterium Strains

JOHN LEE

14 9

14 . Fatty Acid Reductase from Photobacterium phos- ANGEL RODRIGUEZ ,phoreum

LEE WALL ,DENIS RIENDEAU, AN DEDWARD MEIGHEN

17 2

15. Bioluminescence-Related Acyltransferases from DAVID BYERS ,Photobacterium phosphoreum and Vibrio ANGEL RODRIGUEZ ,harveyi

Luc CAREY, AN DEDWARD MEIGHEN

18 3

16. Bioluminescent Analysis of Insect Pheromones

DAVID MORSE ,ROSE SZITTNER ,GARY GRANT, AN DEDWARD MEIGHEN

18 9

17 . Bioluminescent Assays Using Coimmobilized En- G . WIENHAUSEN AN Dzymes

M . DELUCA

19 8

18 . Bioluminescent Assays of Estrogens

JEAN-CLAUDE NICOLAS ,

ANNE MARIE BOUSSIOUX, AN DANDRE CRASTES DE PAULET 20 9

19. Immobilized Enzymes for Assaying Bile Acids

J . SCHOELMERICH, A . RODA ,AND M . DELUCA

21 5

20 . Continuous-Flow Bioluminescent Assays Em- DANIEL C . VELLOM AN Dploying Sepharose-Immobilized Enzymes

LARRY J . KRICKA

22 9

21 . Flow Systems Utilizing Nylon-Immobilized En- ALDO RODA ,zymes

STEFANO GIROTTI ,AND GIACOMO CARREA

23 8

22 . Active Center-Based Immunoassay Approach Us- THOMAS O . BALDWIN ,ing Bacterial Luciferase

THOMAS F . HOLZMAN, AN DRITA B . HOLZMAN

24 8

23. Bioluminescence Test for Genotoxic Agents

S . ULITZUR

26 4

24 . Determination of Antibiotic Activities with the S . ULITZUR

27 5Aid of Luminous Bacteri a

25 . Amplified Bioluminescence Assay Using Avidin- G . BARNARD, E . A . BAYER ,Biotin Technology

M . WILCHEK ,Y . AMIR-ZALTSMAN, AN DF . KOHEN

284

26, Isolation and Expression of a cDNA Coding for DOUGLAS C . PRASHER ,Aequorin, the Ca''-'-Activated Photoprotein RICHARD O . MCCANN, AN Dfrom Aeguorea victoria

MILTON J . CORMIER

28 8

27 . Molecular Cloning of Apoaequorin cDNA

MASATo NOGUCHI ,FREDERICK 1 . Tsuji, AN DYOSHIYUKI SAKAK[

29 8

28 . Cell-Free Components in Dinoflagellate Blolumi- J . WOODLAND HASTINGS AN Dnescence . The Particulate Activity : Scintillons ; JAY C . DUNLAP

30 7The Soluble Components : Luciferase, Lucif-erin, and Luciferin-Binding Protein

Section H . Chemiluminescenc e

29 . Enhanced Chemiluminescent Reactions Catalyzed GARY H . G . THORPE AN Dby Horseradish Peroxidase

LARRY J . KRICKA

33 1

30 . Luminescence Immunoassays for Haptens and W . GRAHAM WOOD ,Proteins

JöRG BRAUN ,AND UWE HANTKE

354

31 . Immunoassays Using Acridinium Esters

IAN WEEKS, MARIA STURGESS ,RICHARD C . BROWN, ANDJ . STUART WOODHEAD

366

32 . Surface Chemiluminescent Immunoassays of Ste- F . KOHEN, J . DE BOEVER ,roids

AND J . B . KIM

387

33 . Photographic Detection of Chemiluminescent and LARRY J . KRICKA AN DBioluminescent Reactions

GARY H . G . THORPE

404

34 . On-Line Computer Analysis of the Kinetics of MARIO PAZZAGLI AN DChemiluminescent Reactions : Application to MARIO SERIO

42 0Luminescent Immunoassays

35 . Chemiluminescence Detection System for High- KAZUHIRO IMAI

43 5Performance Liquid Chromatograph y

36 . Phagocytic Leukocyte Oxygenation Activities and ROBERT C . ALLEN

44 9Chemiluminescence : A Kinetic Approach t oAnalysi s

37 . Cellular Chemiluminescence Associated with Dis- KNOX VAN DYKE AN Dease States

CHRIS VAN DYKE

49 3

38 . Luminescent Bioassays Based on Macrophage PATRICK DE BAETSELIER ANDCell Lines

ERIC SCHRAM

507

39 . Stable 1,2-Dioxetanes as Labels for Thermo- JAN C . HUMMELEN ,chemi luminescent Immunoassay

THEO M . LUIDER, ANDHANS WYNBERG

531

40. Synthesis of Chemiluminogenic Protein Conju- G . MESSERI ,

gates with Dimethyl Adipimidate for Sensitive H . R . SCHROEDER ,

Immunoassays

A . L . CALDIN[, AN D

C . ORLANDO

557

41 . Chemiluminescent Probes for Singlet Oxygen in AMBLER THOMPSON ,

Biological Reactions

HOWARD 11 . SELIGER, AN D

GARY H . POSNER

56 9

Section III . Instrumentation

42. Characteristics of Commercial Radiometers

PHILIP E . STANLEY

58 7

AUTHOR INDEX 605

SUBJECT INDEX 623