Biology 22

DrosophilaDrosophila

Identification of various Identification of various phenotypes.phenotypes.

Reference for Pictures:

Carolina Carolina Drosophila melanogsterDrosophila melanogster Manual Manual (45-2620)(45-2620)

Published: Carolina Biological Supply Published: Carolina Biological Supply CompanyCompany

The life C

ycle

Life Cycle: Drosophila melanogaster

Eggs: contain filaments Eggs: contain filaments

2 molting phases “instars”2 molting phases “instars”

Life cycle is 10 days Life cycle is 10 days

Fruity Fly Egg and Larva

Fruit Fly Pupa and Adult

Identification of:

Gender

Body Color

Wild Type Versus Mutants

Body ColorBody Color

Wild type: BeigeWild type: Beige Mutant: Yellow bodyMutant: Yellow body

Parental phenotypes Experiment: body color

A= female: wt (beige)A= female: wt (beige) B=female: wt (beige)B=female: wt (beige) C=male: wt (beige)C=male: wt (beige) D=female: mutant (yellow)D=female: mutant (yellow) E=male: mutant (yellow)E=male: mutant (yellow)

Fruit Fly Body Color

Sex combs: Male Drosophila

Abdominal segements

7 segments to female abdominal segments7 segments to female abdominal segments

5 segments to male abdominal segments5 segments to male abdominal segments

Male body size is smaller than femaleMale body size is smaller than female

But the “last” abdominal segment is larger in maleBut the “last” abdominal segment is larger in male

Nap TimeTriethylamine

How to anesthetize a fly

How to use the Napper

View the Fruit Fly

You will record class data

After counting the crosses record the data After counting the crosses record the data on the class spreadsheet on the class spreadsheet

3 point test cross: Experiment 2

Experiment 2Experiment 2 3 point test cross3 point test cross

Eye colorEye color Body colorBody color Wing lengthWing length

3 point test cross for:

1. Body Color1. Body Color

2. Wing length2. Wing length

3. Eye Color3. Eye Color

♦♦ Wt vs. Mutant

Beige (bl+) vs. Black (bl)

Long(vg+) vs. Vestigal(vg)

Brick red (br+) vs. brown (br)

Crosses:

Female Female Males Males

Males and female progeny from this F2 cross Males and female progeny from this F2 cross are your 3 point test cross results!are your 3 point test cross results!

Xbl vg bw

bl vg bw

bl+ vg+ Bw+

bl vg bw

There are all sorts of eyes!

Eyes

You will have only red eyes (wt)

Fruit Fly Wings

1866-1945

Parental phenotypes

A= female: wt beige bodyA= female: wt beige body B=female: wt beige bodyB=female: wt beige body C=male: wt beige bodyC=male: wt beige body D=female: yellow bodyD=female: yellow body E=male: yellow bodyE=male: yellow body

Female and Male Fruit Flies