New Biotechnology · Volume 31S · July 2014 NANOBIOTECHNOLOGY

PQ-03

A nanovesicle-based olfactory biosensor and its applica-tion to disease diagnosis and grain quality assessment

Tai Hyun Park ∗, Jong Hyun Lim, Jung Ho Ahn

Seoul National University, South Korea

We integrated the olfactory system to carbon nanotube plat-forms for biosensing applications. Human olfactory receptor(OR)-containing nanovesicles were produced from human embry-onic kidney (HEK)-293 cells. The nanovesicles, which generateolfactory signals through a cAMP pathway, were integrated intosingle-walled carbon nanotubes field-effect transistors (SWNT-FETs). The nanovesicles and SWNT-FETs play roles in perceivingspecific odorants, and in amplifying cellular signals, respectively.In particular, this system can be used for the diagnosis of diseasesuch as lung cancer and also for the real-time monitoring of fungalcontamination in grain. Specific olfactory receptors recognizingthe chemical biomarkers were first selected through screening alibrary of human olfactory receptors. The nanovesicle-integrateddevice was able to detect a lung cancer biomarker (heptanal) and aspecific compound generated from contaminated grain (1-octen-3-ol) with excellent sensitivity and selectivity, similar to the originalolfactory system.

http://dx.doi.org/10.1016/j.nbt.2014.05.888

PQ-04

Biological synthesis of silver nanoparticles using plantleaf extracts and their specific antimicrobial activity

Beom Soo Kim ∗, Bipinchandra Salunke, Shailesh Sawant, BassamAlkotaini

Chungbuk National University, South Korea

Several plant leaf extracts (Kalopanax, Magnolia, Persimmon,Pine, Ginkgo, Platanus, etc.) were used for extracellular syn-thesis of silver nanoparticles. Stable silver nanoparticles wereformed by treating aqueous solution of AgNO3 with the plant leafextracts as reducing agent. The synthesized silver nanoparticleswere characterized by UV-vis spectroscopy, FT-IR, inductively cou-pled plasma spectrometry, energy dispersive X-ray spectroscopy,X-ray photoelectron spectroscopy, high-resolution transmissionelectron microscopy, etc. Antimicrobial susceptibility tests of sil-ver nanoparticle treatments revealed variability in sensitivity ofBacillus cereus and Saccharophagus degradans. Minimum inhibitoryconcentration (MIC) values of the silver nanoparticles for B. cereusand S. degradans were found to be 30 �g/mL and 10 �g/mL, respec-tively. The mixed culture of B. cereus and S. degradans treated withsilver nanoparticles at 10 �g/mL after 24 h showed presence of onlyB. cereus colonies. This study suggests that plant leaf extract syn-thesized silver nanoparticles can selectively inhibit growth of theGram negative S. degradans and retain the Gram positive B. cereusat MIC values of S. degradans.

http://dx.doi.org/10.1016/j.nbt.2014.05.889

PQ-05

Biosynthesis of single nanoparticles using various metalbinding proteins

Yoojin Choi ∗, Sang Yup Lee, Doh Chang Lee

Korea Advanced Institute of Science and Technology, South Korea

Recently nanotechnology has attracted attention worldwidebecause of the interesting physicochemical properties of these par-ticles. However, most nanoparticles are chemically synthesizedand involve the use of expensive catalysts for reactions at hightemperature and pressure. The environmental issues related tothe synthesis of nanoparticles have motivated research towardgreener methods that utilize microorganisms such as bacteria,yeast, and fungi for their ability to reduce metal ions. We syn-thesized various single nanoparticles using metal binding proteinson recombinant Escherichia coli (E. coli). The morphology andsize of the synthesized nanoparticles was observed by low tohigh resolution transmission electron microscopy (TEM) at 200 kVand energy-dispersive X-ray (EDX) spectra. Finally, we suggest apossible mechanism of the biosynthesis process that might pro-vide a guide for conditions required for the synthesis of variousnanoparticles.

http://dx.doi.org/10.1016/j.nbt.2014.05.890

PQ-06

Electro-triggered, spatioselective, quantitative genedelivery into a single cell nucleus by Au nanowirenanoinjector

Seung Min Yoo ∗, Sang Yup Lee

KAIST, South Korea

Delivery of bioactive materials into a cell is highly impor-tant in the study of cell biology and medical treatments.Ideal nanoinjectors should be able to deliver biomaterials withhigh spatial resolution while causing minimum cell damage.We developed a Au nanowire (NW) nanoinjector that has thethinnest diameter among the DNA delivering devices as wellas optimum mechanical properties, minimizing cell damage.Well-defined single-crystalline Au surface and high electric con-ductivity of a Au NW nanoinjector allow precisely timed andefficient electrochemical release of DNA molecules attachedon a Au NW surface. Both linear DNA and plasmid DNAwere delivered separately, and showed successful expression.The Au NW nanoinjector would find important biomedicalapplications in the fields such as gene therapy, DNA vaccina-tion, targeted drug delivery, and probe/control of cell signalingevents [1].

Acknowledgements: This work was supported by theTechnology Development Program to Solve Climate Changeson Systems Metabolic Engineering for Biorefineries (NRF-2012-C1AAA001-2012M1A2A2026556) of the Ministry of Education,Science and Technology (MEST) through the National ResearchFoundation of Korea.

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